Acta Histochemica Et Cytochemica最新文献_第8页

Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding. 酶标抗原法:影响福尔马林固定和石蜡包埋过程中特异性抗体抗原结合活性下降的因素。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00023

Yasuyoshi Mizutani, Kazuya Shiogama, Ken-Ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi

{"title":"Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding.","authors":"Yasuyoshi Mizutani, Kazuya Shiogama, Ken-Ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi","doi":"10.1267/ahc.22-00023","DOIUrl":"https://doi.org/10.1267/ahc.22-00023","url":null,"abstract":"The enzyme-labeled antigen method is an immunohistochemical technique detecting plasma cells producing specific antibodies in tissue sections. The probe is an antigen labeled with an enzyme or biotin. This immunohistochemical technique is appliable to frozen sections of paraformaldehyde (PFA)-fixed tissues, but it has been difficult to apply it to formalin-fixed, paraffin-embedded (FFPE) sections. In the current study, factors inactivating the antibody reactivity during the process of preparing FFPE sections were investigated. Lymph nodes of rats immunized with horseradish peroxidase (HRP) or a mixture of keyhole limpet hemocyanin/ovalbumin/bovine serum albumin were employed as experimental models. Plasma cells producing specific antibodies, visualized with HRP (as an antigen with enzymatic activity) or biotinylated proteins in 4% PFA-fixed frozen sections, significantly decreased in unbuffered 10% formalin-fixed frozen sections. The positive cells were further decreased by paraffin embedding following formalin fixation. In paraffin-embedded sections fixed in precipitating fixatives such as ethanol and acetone and those prepared with the AMeX method, the antigen-binding reactivity of antibodies was preserved. Fixation in periodate-lysine-paraformaldehyde and Zamboni solution also kept the antigen-binding reactivity in paraffin to some extent. In conclusion, formalin fixation and paraffin embedding were major causes inactivating antibodies. Precipitating fixatives could retain the antigen-binding reactivity of antibodies in paraffin-embedded sections.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":" ","pages":"129-148"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/3b/ahc-055-129.PMC9631987.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40698207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats. 艾布selen对大鼠上腹部皮瓣缺血再灌注损伤的保护作用。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00062

Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu

{"title":"Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats.","authors":"Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu","doi":"10.1267/ahc.22-00062","DOIUrl":"https://doi.org/10.1267/ahc.22-00062","url":null,"abstract":"The purpose of this study was to determine the role of oxidized diacylglycerol (DAG) and the molecular mechanism underlying ischemia-reperfusion (I/R) injury in rat skin flaps. The protective effect of ebselen on the viability of rat skin flaps with I/R injury was investigated. Flaps were designed and raised in the left inguinal region. Then, a microvascular clamp was applied to the vascular pedicle and reperfused after 6 hr. After 7 days of I/R (I/R group), the skin flap survival area ratio was significantly reduced compared to the normal skin. The administration of ebselen significantly improved the ratio compared to the I/R group. The flap survival area ratio of the I/R + ebselen group was significantly improved compared to the I/R + vehicle group. In the I/R + ebselen group, the oxidized DAG content and intensity of phosphorylated PKCα and PKCδ were significantly lower compared to the I/R + vehicle group. Furthermore, the inflammatory response was suppressed in the I/R + ebselen group compared to the I/R + vehicle group. These results indicate that ebselen is useful as a preventive and therapeutic agent for skin flap necrosis caused by I/R, because of reduction and elimination of oxidized DAG.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":" ","pages":"149-157"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/c2/ahc-055-149.PMC9631984.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40697214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphologic Analysis of M2 Macrophage in Glioblastoma: Involvement of Macrophage Extracellular Traps (METs). 胶质母细胞瘤中 M2 巨噬细胞的形态学分析：巨噬细胞胞外陷阱 (MET) 的参与。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-08-27 Epub Date: 2022-08-10 DOI: 10.1267/ahc.22-00018

Ayano Michiba, Kazuya Shiogama, Tetsuya Tsukamoto, Masaya Hirayama, Seiji Yamada, Masato Abe

{"title":"Morphologic Analysis of M2 Macrophage in Glioblastoma: Involvement of Macrophage Extracellular Traps (METs).","authors":"Ayano Michiba, Kazuya Shiogama, Tetsuya Tsukamoto, Masaya Hirayama, Seiji Yamada, Masato Abe","doi":"10.1267/ahc.22-00018","DOIUrl":"10.1267/ahc.22-00018","url":null,"abstract":"Macrophages are classified into two phenotypes, M1 and M2, based on their roles. M2 macrophages suppress inflammation and increase in proportion to the malignancy of brain tumors. Recently, macrophage extracellular traps (METs), which change into a network, have been reported as a unique form of macrophage cell death. In this study, immunohistochemical analysis of macrophages in METs in human glioblastoma was performed. To distinguish between M1 and M2 macrophages, multiple immunostainings with Iba1 combined with CD163 or CD204 were performed. M2 macrophages were present in small amounts in normal and borderline areas but showed an increasing trend as they shifted to tumor areas, and most of them were the activated- or phagocytic-type. We also successfully detected METs coexisting with fibrin and lactoferrin near the border between the tumor and necrotic area. M2 macrophages not only suppressed inflammation but also were involved in the formation of METs. This study found that M2 macrophages play various roles in unstable situations.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 4","pages":"111-118"},"PeriodicalIF":1.6,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/5c/ahc-055-111.PMC9427541.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40349780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EPLINβ Is Involved in the Assembly of Cadherin-catenin Complexes in Osteoblasts and Affects Bone Formation. EPLINβ参与成骨细胞钙粘蛋白-连环蛋白复合物的组装并影响骨形成

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-06-25 DOI: 10.1267/ahc.22-00027

Shihoko Miyazaki, Taro Funamoto, Tomohisa Sekimoto, Syuji Kurogi, Tomomi Ohta, Takuya Nagai, Takuya Tajima, Mai Imasaka, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Narantsog Choijookhuu, Yoshitaka Hishikawa, Etsuo Chosa

{"title":"EPLINβ Is Involved in the Assembly of Cadherin-catenin Complexes in Osteoblasts and Affects Bone Formation.","authors":"Shihoko Miyazaki, Taro Funamoto, Tomohisa Sekimoto, Syuji Kurogi, Tomomi Ohta, Takuya Nagai, Takuya Tajima, Mai Imasaka, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Narantsog Choijookhuu, Yoshitaka Hishikawa, Etsuo Chosa","doi":"10.1267/ahc.22-00027","DOIUrl":"https://doi.org/10.1267/ahc.22-00027","url":null,"abstract":"Epithelial protein lost in neoplasm (EPLIN) is an actin-associated cytoskeletal protein that plays an important role in epithelial cell adhesion. EPLIN has two isoforms: EPLINα and EPLINβ. In this study, we investigated the role of EPLINβ in osteoblasts using EPLINβ-deficient (EPLINβGT/GT ) mice. The skeletal phenotype of EPLINβGT/GT mice is indistinguishable from the wildtype (WT), but bone properties and strength were significantly decreased compared with WT littermates. Histomorphological analysis revealed altered organization of bone spicules and osteoblast cell arrangement, and decreased alkaline phosphatase activity in EPLINβGT/GT mouse bones. Transmission electron microscopy revealed wider intercellular spaces between osteoblasts in EPLINβGT/GT mice, suggesting aberrant cell adhesion. In EPLINβGT/GT osteoblasts, α- and β-catenins and F-actin were observed at the cell membrane, but OB-cadherin was localized at the perinuclear region, indicating that cadherin-catenin complexes were not formed. EPLINβ knockdown in MC3T3-e1 osteoblast cells showed similar results as in calvaria cell cultures. Bone formation markers, such as RUNX2, Osterix, ALP, and Col1a1 mRNA were reduced in EPLINβ knockdown cells, suggesting an important role for EPLINβ in osteoblast formation. In conclusion, we propose that EPLINβ is involved in the assembly of cadherin-catenin complexes in osteoblasts and affects bone formation.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 3","pages":"99-110"},"PeriodicalIF":2.4,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/ca/ahc_055-99.PMC9253499.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40587490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Current Status of Whole Slide Image (WSI) Standardization in Japan. 日本全幻灯片图像(WSI)标准化现状。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-06-25 DOI: 10.1267/ahc.22-00009

Ichiro Mori

引用次数: 1

A Histochemical Analysis of Neurofibrillary Tangles in Olfactory Epithelium, a Study Based on an Autopsy Case of Juvenile Alzheimer's Disease. 嗅觉上皮中神经原纤维缠结的组织化学分析，基于一例青少年阿尔茨海默病的尸检研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-06-25 DOI: 10.1267/ahc.22-00048

Shino Shimizu, Ichiro Tojima, Keigo Nakamura, Hideaki Kouzaki, Takeshi Kanesaka, Norihiro Ogawa, Yoshio Hashizume, Hiroyasu Akatsu, Akira Hori, Ikuo Tooyama, Takeshi Shimizu

{"title":"A Histochemical Analysis of Neurofibrillary Tangles in Olfactory Epithelium, a Study Based on an Autopsy Case of Juvenile Alzheimer's Disease.","authors":"Shino Shimizu, Ichiro Tojima, Keigo Nakamura, Hideaki Kouzaki, Takeshi Kanesaka, Norihiro Ogawa, Yoshio Hashizume, Hiroyasu Akatsu, Akira Hori, Ikuo Tooyama, Takeshi Shimizu","doi":"10.1267/ahc.22-00048","DOIUrl":"https://doi.org/10.1267/ahc.22-00048","url":null,"abstract":"The pathological changes of Alzheimer's disease (AD) begin 10-20 years before clinical onset, and it is therefore desirable to identify effective methods for early diagnosis. The nasal mucosa is a target tissue for measuring AD-related biomarkers because the olfactory nerve is the only cranial nerve that is exposed to the external environment. We describe an autopsy case of rapidly advanced juvenile AD (JAD), focusing on the olfactory system. The formation of senile plaques, neurofibrillary tangles (NFTs), and neuropil threads was examined in the temporal cortex, hippocampus, olfactory bulb, and olfactory and respiratory epithelia in the bilateral olfactory clefts. Neurodegenerative changes in the olfactory and respiratory epithelia and the pathological deposition of amyloid β42 (Aβ42) and phosphorylated tau were also examined. As a result, senile plaques, NFTs, and neuropil threads were found in the temporal cortex, hippocampus, and olfactory bulb. NFTs were also found in the olfactory epithelium. Degenerated olfactory cells and their axons stained positive for phosphorylated tau. Supporting cells in the degenerated olfactory epithelium stained positive for Aβ42. In conclusion, pathological biomarkers of AD were expressed in the degenerated olfactory epithelium of this JAD patient. This observation suggests that nasal samples may be useful for the diagnosis of AD.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 3","pages":"93-98"},"PeriodicalIF":2.4,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/d7/ahc_055-93.PMC9253500.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40587491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pulmonary Neuroendocrine Cells and Small Cell Lung Carcinoma: Immunohistochemical Study Focusing on Mechanisms of Neuroendocrine Differentiation. 肺神经内分泌细胞与小细胞肺癌:神经内分泌分化机制的免疫组化研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-05-24 DOI: 10.1267/ahc.22-00031

Takaaki Ito, Shinji Kudoh, Kosuke Fujino, Mune Sanada, Yuki Tenjin, Haruki Saito, Yuko Nakaishi-Fukuchi, Hiroki Kameyama, Takaya Ichimura, Naoko Udaka, Noritaka Kudo, Akira Matsuo, Younosuke Sato

{"title":"Pulmonary Neuroendocrine Cells and Small Cell Lung Carcinoma: Immunohistochemical Study Focusing on Mechanisms of Neuroendocrine Differentiation.","authors":"Takaaki Ito, Shinji Kudoh, Kosuke Fujino, Mune Sanada, Yuki Tenjin, Haruki Saito, Yuko Nakaishi-Fukuchi, Hiroki Kameyama, Takaya Ichimura, Naoko Udaka, Noritaka Kudo, Akira Matsuo, Younosuke Sato","doi":"10.1267/ahc.22-00031","DOIUrl":"https://doi.org/10.1267/ahc.22-00031","url":null,"abstract":"Neuroendocrine (NE) differentiation has been histochemically detected in normal and cancer tissues and cells. Immunohistochemical analyses have provided a more detailed understanding of NE biology and pathology. Pulmonary NE cells are a rare lung epithelial type, and small cell carcinoma of the lung (SCLC) is a high-grade NE tumor. Pulmonary NE and SCLC cells share common mechanisms for NE differentiation. Neural or NE cell lineage-specific transcription factors, such as achaete-scute homologue 1 (Ascl1) and insulinoma-associated protein 1 (INSM1), are crucial for the development of pulmonary NE cells, and NE differentiation is influenced by the balance between Ascl1 and the suppressive neural transcription factor, hairy-enhancer of split 1, a representative target molecule of the Notch signaling pathway. In this review, we discuss the importance of Ascl1 and INSM1 in identifying pulmonary NE and SCLC cells and introduce Ascl1-related molecules detected by comparative RNA-sequence analyses. The molecular classification of SCLC based on the expression of lineage-specific transcription or co-transcription factors, including ASCL1, NEUROD1, POU2F3, and YAP1, was recently proposed. We attempted to characterize these 4 SCLC subtypes using integrated immunohistochemical studies, which will provide insights into the molecular characteristics of these subtypes and clarify the inter- and intratumor heterogeneities of SCLC.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 3","pages":"75-83"},"PeriodicalIF":2.4,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ee/bd/ahc_055-75.PMC9253501.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40587494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Colonic Lymphoid Follicle Hyperplasia after Gastrectomy in Rats 大鼠胃切除术后结肠淋巴滤泡增生

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-04-23 DOI: 10.1267/ahc.22-00015

Tomoko Kagiya, K. Shiogama, K. Inada, H. Utsunomiya, Masayuki Kitano

{"title":"Colonic Lymphoid Follicle Hyperplasia after Gastrectomy in Rats","authors":"Tomoko Kagiya, K. Shiogama, K. Inada, H. Utsunomiya, Masayuki Kitano","doi":"10.1267/ahc.22-00015","DOIUrl":"https://doi.org/10.1267/ahc.22-00015","url":null,"abstract":"Nodular lymphoid hyperplasia (NLH) of the human colon has been associated with multiple diseases and symptoms. Causes include food allergies, infections, inflammatory bowel disease, and immunodeficiency, and gastrectomy is not usually considered to be the etiology. Nine rats two weeks after total gastrectomy and 12 control rats were sacrificed and submitted for histological examination. In the gastrectomy group, we found lymphoid hyperplasia throughout the entire colon mucosa. The cross-sectional area of lymphoid follicles was increased to be five-fold larger than that in the rats in the control group (sham surgery). Lymphoid follicles were classified into primary and secondary follicles according to the presence/absence of germinal centers; the gastrectomy group had a significantly larger number of secondary follicles. When T cell and B cell classification of lymphocytes was performed, there was no difference between gastrectomy and control groups at T:B = 40:60. When the lymphoid follicles were classified, the proportion of T lymphocytes increased in the secondary follicle (T:B = 40:60) compared with in the primary follicle (T:B = 20:80). Gastrectomy significantly activated lymphocytic intestinal immunity by altering the intestinal environment, causing colonic NLH. Gastrectomy in rats is a good animal model for the study of NLH in colorectal diseases.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 1","pages":"67 - 73"},"PeriodicalIF":2.4,"publicationDate":"2022-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48050915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histomorphological Changes in a Rat Model of Polycystic Ovary Syndrome and the Contribution of Stevia Leaf Extract in Modulating the Ovarian Fibrosis, VEGF, and TGF-β Immunoexpressions: Comparison with Metformin. 多囊卵巢综合征大鼠模型的组织形态学改变及甜菊叶提取物对卵巢纤维化、VEGF和TGF-β免疫表达的调节作用:与二甲双胍的比较

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-02-26 Epub Date: 2022-01-27 DOI: 10.1267/ahc.21-00081

Ahmed A Morsi, Ezat A Mersal, Abdel Razik H Farrag, Ahmed M Abdelmoneim, Alshaymaa M Abdelmenem, Mohamed S Salim

{"title":"Histomorphological Changes in a Rat Model of Polycystic Ovary Syndrome and the Contribution of Stevia Leaf Extract in Modulating the Ovarian Fibrosis, VEGF, and TGF-β Immunoexpressions: Comparison with Metformin.","authors":"Ahmed A Morsi, Ezat A Mersal, Abdel Razik H Farrag, Ahmed M Abdelmoneim, Alshaymaa M Abdelmenem, Mohamed S Salim","doi":"10.1267/ahc.21-00081","DOIUrl":"10.1267/ahc.21-00081","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a common endocrine disorder of fertile females. It has been reported that stevia leaf extract (SLE) has antidiabetic and antihyperlipidemic properties. Therefore, the current study hypothesized and investigated the role and mechanistic aspects of a natural sweetener; SLE in treating a rat model of letrozole-induced PCOS and to compare it with metformin. Thirty-five female Wistar albino rats were divided into 5 groups: control, PCOS-induced group (letrozole, 1 mg/kg/d, for 21 days), SLE, metformin, and combination-treated groups (300 mg/kg/d, for the next 28 days in SLE and metformin-treated groups). Vaginal smears were done. The levels of glucose, lipid, and hormonal profiles were measured in the serum meanwhile, malonyl dialdehyde (MDA), superoxide dismutase (SOD), and tumour necrosis factor (TNF-α) were measured in the ovary. Ovarian sections were subjected to hematoxylin and eosin, Masson, and immunohistochemical identification of VEGF and TGF-β followed by morphometric analysis. PCOS rats showed altered hormonal and lipid profiles, in addition to hyperglycemia. Also, the ovarian tissue levels of MDA and TNF-α were elevated, and SOD was decreased. Numerous cystic follicles, decrease/absence of corpora lutea, interstitial fibrosis with positive VEGF and TGF-β immunoreactivity were evident. SLE improved all altered parameters. SLE showed potential therapeutic merits in letrozole-induced PCOS via anti-inflammatory, antioxidant, anti-fibrotic, and angiogenesis regulating mechanisms. Its effects were almost comparable to metformin, and the combination of both has no further synergistic effect.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 1","pages":"9-23"},"PeriodicalIF":2.4,"publicationDate":"2022-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42370014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Brain Regions Activated by Sevoflurane and Propofol and Regional Changes in Gene Expression 七氟醚和异丙酚激活脑区的鉴定及基因表达的区域变化

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-02-22 DOI: 10.1267/ahc.21-00091

N. Kamei, Shimpei Higo, Tomoki Mizuno, Keisuke Mori, A. Sakamoto, H. Ozawa

{"title":"Identification of Brain Regions Activated by Sevoflurane and Propofol and Regional Changes in Gene Expression","authors":"N. Kamei, Shimpei Higo, Tomoki Mizuno, Keisuke Mori, A. Sakamoto, H. Ozawa","doi":"10.1267/ahc.21-00091","DOIUrl":"https://doi.org/10.1267/ahc.21-00091","url":null,"abstract":"General anesthetics have different efficacies and side effect incidences based on their mechanism of action. However, detailed comparative studies of anesthetics are incomplete. In this study, target brain regions and gene expression changes in these brain regions were determined for sevoflurane and propofol to understand the mechanisms that cause differences among anesthetics. Rats were anesthetized with sevoflurane or propofol for 1 hr, and brain regions with anesthesia-induced changes in neuronal activity were examined by immunohistochemistry and in situ hybridization for c-Fos. Among the identified target brain regions, gene expression analysis was performed in the habenula, the solitary nucleus and the medial vestibular nucleus from laser microdissected samples. Genes altered by sevoflurane and propofol were different and included genes involved in the incidence of postoperative nausea and vomiting and emergence agitation, such as Egr1 and Gad2. GO enrichment analysis showed that the altered genes tended to be evenly distributed in all functional category. The detailed profiles of target brain regions and induced gene expression changes of sevoflurane and propofol in this study will provide a basis for analyzing the effects of each anesthetic agent and the risk of adverse events.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 1","pages":"37 - 46"},"PeriodicalIF":2.4,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49543893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2