Acta Histochemica Et Cytochemica最新文献_第8页

Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT. 代谢重编程通过TERT的表观遗传调控驱动垂体肿瘤生长。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-06-29 Epub Date: 2021-06-23 DOI: 10.1267/ahc.21-00007

Hiromi Onizuka, Kenta Masui, Kosaku Amano, Takakazu Kawamata, Tomoko Yamamoto, Yoji Nagashima, Noriyuki Shibata

{"title":"Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT.","authors":"Hiromi Onizuka, Kenta Masui, Kosaku Amano, Takakazu Kawamata, Tomoko Yamamoto, Yoji Nagashima, Noriyuki Shibata","doi":"10.1267/ahc.21-00007","DOIUrl":"https://doi.org/10.1267/ahc.21-00007","url":null,"abstract":"Pituitary adenomas are common, benign brain tumors. Some tumors show aggressive phenotypes including early recurrence, local invasion and distant metastasis, but the underlying mechanism to drive the progression of pituitary tumors has remained to be clarified. Aerobic glycolysis known as the Warburg effect is one of the emerging hallmarks of cancer, which has an impact on the tumor biology partly through epigenetic regulation of the tumor-promoting genes. Here, we demonstrate metabolic reprogramming in pituitary tumors contributes to tumor cell growth with epigenetic changes such as histone acetylation. Notably, a shift in histone acetylation increases the expression of telomerase reverse transcriptase (TERT) oncogene, which drives metabolism-dependent cell proliferation in pituitary tumors. These indicate that epigenetic changes could be the specific biomarker for predicting the behavior of pituitary tumors and exploitable as a novel target for the aggressive types of the pituitary tumors.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 3","pages":"87-96"},"PeriodicalIF":2.4,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/f7/ahc-054-87.PMC8275863.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39195137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Immunohistochemical Study of Mitochondrial Ferritin in the Midbrain of Patients with Progressive Supranuclear Palsy. 进行性核上性麻痹患者中脑线粒体铁蛋白的免疫组化研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-06-29 Epub Date: 2021-06-23 DOI: 10.1267/ahc.21-00019

Zulzikry Hafiz Abu Bakar, Tomoko Kato, Daijiro Yanagisawa, Jean-Pierre Bellier, Ken-Ichi Mukaisho, Ikuo Tooyama

{"title":"Immunohistochemical Study of Mitochondrial Ferritin in the Midbrain of Patients with Progressive Supranuclear Palsy.","authors":"Zulzikry Hafiz Abu Bakar, Tomoko Kato, Daijiro Yanagisawa, Jean-Pierre Bellier, Ken-Ichi Mukaisho, Ikuo Tooyama","doi":"10.1267/ahc.21-00019","DOIUrl":"https://doi.org/10.1267/ahc.21-00019","url":null,"abstract":"Mitochondrial ferritin (FtMt) is a novel ferritin that is localized in the mitochondria. FtMt expression is low in the liver and spleen, and high in the heart, testis, and brain. We previously detected FtMt in dopaminergic neurons in the substantia nigra pars compacta (SNc) in human and monkey midbrains. We investigated the localization and expression of FtMt in the midbrain of patients with progressive supranuclear palsy (PSP) and controls using a monoclonal antibody (C65-2) against human FtMt. FtMt immunoreactivity was weakly detected in neuromelanin-containing neurons in the SNc and ventral tegmental area (VTA) of control cases compared with PSP, which exhibited a remarkable increase in FtMt immunoreactivity. Preincubation of C65-2 with the immunizing FtMt peptide significantly reduced the staining, indicating the specificity of C65-2. Several puncta were observed outside the neurons of PSP, in contrast with the control cases. Double immunofluorescence histochemistry for FtMt and tyrosine hydroxylase (TH), glial fibrillary acidic protein, and Iba1 showed localization of FtMt in dopaminergic neurons, microglia, and astrocytes in PSP. Furthermore, FtMt immunoreactivity was detected in a few TH-negative neurons. In the SNc and VTA, FtMt immunoreactivity colocalized with phosphorylated tau immunoreactivity. Our results indicate that FtMt is involved in the pathology of PSP. Clarifying the involvement of FtMt in PSP is of great interest.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 3","pages":"97-104"},"PeriodicalIF":2.4,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/11/ed/ahc-054-97.PMC8275861.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39195138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Review of miR-326 and Female Related Diseases. miR-326与女性相关疾病研究进展

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-06-29 Epub Date: 2021-04-22 DOI: 10.1267/ahc.20-00027

Li-Na Lin, Qing-Mei Zhang, Ying-Ying Ge, Bin Luo, Xiao-Xun Xie

{"title":"A Review of miR-326 and Female Related Diseases.","authors":"Li-Na Lin, Qing-Mei Zhang, Ying-Ying Ge, Bin Luo, Xiao-Xun Xie","doi":"10.1267/ahc.20-00027","DOIUrl":"https://doi.org/10.1267/ahc.20-00027","url":null,"abstract":"MicroRNA (miRNA), a non-coding single-stranded RNA molecule with 20-23 nucleotides encoded by endogenous genes, plays an essential role in maintaining normal cell function and regulating cell proliferation, differentiation, apoptosis, autophagy, and cell metabolism. The imbalance between miRNA and genes can cause a series of diseases, including malignancies. miRNA-326 (miR-326) is extensively known for its core regulation of various biological processes. This review presents an overview of the highlights of miR-326 in female-related diseases. To understand the impact of miR-326 on female disorders, we search all published studies about miR-326 having a high incidence in female conditions, including cervical cancer, endometrial cancer, breast cancer, intrauterine adhesion, and multiple autoimmune diseases. We aim to learn about the mutual regulation mechanism between miR-326 and related genes and signaling pathways, as well as to elaborate on the value of miR-326 as a potential biomarker and therapeutic target of female diseases. Our results provide reliable evidence and new strategies for treating female tumors and autoimmune diseases.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 3","pages":"79-86"},"PeriodicalIF":2.4,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/bd/ahc-054-79.PMC8275862.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39196704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

What Cyto- and Histochemistry Can Do to Crack the Sugar Code. 细胞和组织化学如何破解糖密码?

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-04-17 DOI: 10.1267/ahc.21-00017

Felix A Habermann, Herbert Kaltner, Alonso M Higuero, Gabriel García Caballero, Anna-Kristin Ludwig, Joachim C Manning, José Abad-Rodríguez, Hans-Joachim Gabius

{"title":"What Cyto- and Histochemistry Can Do to Crack the Sugar Code.","authors":"Felix A Habermann, Herbert Kaltner, Alonso M Higuero, Gabriel García Caballero, Anna-Kristin Ludwig, Joachim C Manning, José Abad-Rodríguez, Hans-Joachim Gabius","doi":"10.1267/ahc.21-00017","DOIUrl":"10.1267/ahc.21-00017","url":null,"abstract":"As letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo- and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto- and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins), hereby revealing its dynamic spatiotemporal regulation: these data support the concept of a sugar code. When proceeding from work with plant (haem)agglutinins as such tools to the discovery of endogenous (tissue) lectins, it became clear that a broad panel of biological meanings can indeed be derived from the sugar-based vocabulary (the natural glycome incl. post-synthetic modifications) by glycan-lectin recognition in situ. As consequence, the immunocyto- and histochemical analysis of lectin expression is building a solid basis for the steps toward tracking down functional correlations, for example in processes leading to cell adhesion, apoptosis, autophagy or growth regulation as well as targeted delivery of glycoproteins. Introduction of labeled tissue lectins to glycan profiling assists this endeavor by detecting counterreceptor(s) in situ. Combining these tools and their applications strategically will help to take the trip toward the following long-range aim: to compile a dictionary for the glycan vocabulary that translates each message (oligosaccharide) into its bioresponse(s), that is to crack the sugar code.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 2","pages":"31-48"},"PeriodicalIF":2.4,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/23/ahc-054-31.PMC8116616.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38999690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts. 兰根多夫灌注缺血大鼠心脏心肌活力的拉曼光谱评价。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-04-17 DOI: 10.1267/ahc.21-00016

Koki Ikemoto, Kosuke Hashimoto, Yoshinori Harada, Yasuaki Kumamoto, Michiyo Hayakawa, Kentaro Mochizuki, Kazuhiko Matsuo, Kenta Yashiro, Hitoshi Yaku, Tetsuro Takamatsu, Hideo Tanaka

{"title":"Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts.","authors":"Koki Ikemoto, Kosuke Hashimoto, Yoshinori Harada, Yasuaki Kumamoto, Michiyo Hayakawa, Kentaro Mochizuki, Kazuhiko Matsuo, Kenta Yashiro, Hitoshi Yaku, Tetsuro Takamatsu, Hideo Tanaka","doi":"10.1267/ahc.21-00016","DOIUrl":"https://doi.org/10.1267/ahc.21-00016","url":null,"abstract":"Spontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of myocardial viability in ischemic hearts. To address this issue, we investigated sequential changes in Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart before and during ligation of the left coronary artery and its subsequent release and re-ligation. Under 532-nm wavelength excitation, the Raman peak intensity of reduced cytochrome c at 747 cm-1 increased quickly after the coronary ligation, and reached a quasi-steady state within 30 min. Subsequent reperfusion of the heart after a short-term (30-min) ligation that simulates reversible conditions resulted in quick recovery of the peak intensity to the baseline. Further re-ligation resulted in resurgence of the peak intensity to nearly the identical value to the first ischemia value. In contrast, reperfusion after prolonged (120-min) ligation that assumes irreversible states resulted in incomplete recovery of the peak intensity, and re-ligation resulted in inadequate resurgence. Electron microscopic observations confirmed the spectral findings. Together, the Raman spectroscopic measurement for cytochrome c could be applicable to evaluation of viability of the ischemic myocardium without labeling.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 2","pages":"65-72"},"PeriodicalIF":2.4,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/f7/ahc-054-65.PMC8116620.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38999692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of Pathological Diagnosis Support System Using Micro-computed Tomography. 微计算机断层扫描病理诊断支持系统的研制。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-04-22 DOI: 10.1267/ahc.20-00033

Tomonari Hayakawa, Atsushi Teramoto, Yuka Kiriyama, Tetsuya Tsukamoto, Ayumi Yamada, Kuniaki Saito, Hiroshi Fujita

{"title":"Development of Pathological Diagnosis Support System Using Micro-computed Tomography.","authors":"Tomonari Hayakawa, Atsushi Teramoto, Yuka Kiriyama, Tetsuya Tsukamoto, Ayumi Yamada, Kuniaki Saito, Hiroshi Fujita","doi":"10.1267/ahc.20-00033","DOIUrl":"https://doi.org/10.1267/ahc.20-00033","url":null,"abstract":"In pathological diagnosis, the cutting position of pathological materials is subjectively determined by pathologists. This leads to a low cutting accuracy, which in turn may lead to incorrect diagnoses. In this study, we developed a system that supports the determination of the cutting position by visualizing and analyzing the internal structure of pathological material using micro-computed tomography (CT) before cutting. This system consists of a dedicated micro-CT and cutting support software. The micro-CT system has a fixture for fixing the target, enabling the scanning of easily deformable pathological materials. In the cutting support software, a function that interactively selects the extraction plane while displaying the volume rendering image and outputs a pseudo-histological image was implemented. Our results confirmed that the pseudo-histological image showed the fine structure inside the organ and that the latter image was highly consistent with the pathological image.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 2","pages":"49-56"},"PeriodicalIF":2.4,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/70/4c/ahc-054-49.PMC8116619.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38999689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia. 宫颈上皮内瘤变临床过程中HLAⅰ类免疫组织化学表达模式的改变。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-04-17 DOI: 10.1267/ahc.21-00010

Okuto Koguchi, Haruna Nishimaki, Yoko Nakanishi, Hiroko Kobayashi, Sumie Ohni, Xiaoyan Tang, Yoshiaki Kusumi, Shinobu Masuda

{"title":"Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia.","authors":"Okuto Koguchi, Haruna Nishimaki, Yoko Nakanishi, Hiroko Kobayashi, Sumie Ohni, Xiaoyan Tang, Yoshiaki Kusumi, Shinobu Masuda","doi":"10.1267/ahc.21-00010","DOIUrl":"https://doi.org/10.1267/ahc.21-00010","url":null,"abstract":"It is unclear how immunohistochemical expression patterns of HLA class I in the pre-malignant phase of cervical intraepithelial neoplasia (CIN) alter during the clinical follow-up period. The present study aimed to demonstrate the correlation between the immunohistochemical expression pattern of HLA class I and the CIN grade through repeated examinations during the clinical course. Expression patterns of HLA class I, p16INK4a, and PD-L1 were immunohistochemically examined using formalin-fixed paraffin-embedded (FFPE) sections of biopsy or conization samples that were obtained from 20 patients diagnosed with CIN. The mRNA expression levels of HLA class I were analyzed by real-time reverse transcription polymerase chain reaction using FFPE sections of 14 patients, who were examined metachronously during the follow-up period. HLA class I expression was limited to the lower part of the epithelial thickness (M1 pattern) in more than half of CIN1 cases, and was present throughout the epithelial thickness (M2 pattern) in one fourth of CIN1 and CIN2 cases approximately. Heterogeneous expression (H pattern) was detected in half of CIN2 and CIN3 cases and in the all of squamous cell carcinoma cases. Metachronous examinations revealed that these immunohistochemical patterns altered more frequently than the CIN grade. The rate of change of HLA class I mRNA expression level was higher in cases with a progressed immunohistochemical pattern compared to those with regressed immunohistochemical pattern. In conclusion, the immunohistochemical pattern of HLA class I expression is associated with the CIN grade, and it is alterable during the clinical course, especially in CIN2.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 2","pages":"57-64"},"PeriodicalIF":2.4,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/40/ahc-054-57.PMC8116618.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38999691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation with Fermented Barley Extract Prevents Mammary Epithelial Cell Invasion in an Early Breast Cancer Model. 在早期乳腺癌模型中补充发酵大麦提取物可防止乳腺上皮细胞侵袭。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-02-27 DOI: 10.1267/ahc.20-00029

Junji Itou, Akihiro Nakamura, Hideki Hokazono, Masakazu Toi

引用次数: 0

The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice. 牙周损伤诱导C57BL/6J小鼠三叉神经中脑核神经元细胞死亡和三叉神经运动核神经退行性变。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-02-25 Epub Date: 2021-02-16 DOI: 10.1267/ahc.20-00036

Ashis Dhar, Eriko Kuramoto, Makoto Fukushima, Haruki Iwai, Atsushi Yamanaka, Tetsuya Goto

{"title":"The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice.","authors":"Ashis Dhar, Eriko Kuramoto, Makoto Fukushima, Haruki Iwai, Atsushi Yamanaka, Tetsuya Goto","doi":"10.1267/ahc.20-00036","DOIUrl":"https://doi.org/10.1267/ahc.20-00036","url":null,"abstract":"Proprioception from masticatory apparatus and periodontal ligaments comes through the trigeminal mesencephalic nucleus (Vmes). We evaluated the effects of tooth loss on neurodegeneration of the Vmes and trigeminal motor nucleus (Vmo). Bilateral maxillary molars of 2-month-old C57BL/6J mice were extracted under anesthesia. Neural projections of the Vmes to the periodontium were confirmed by injecting Fluoro-Gold (FG) retrogradely into the extraction sockets, and for the anterograde labeling adeno-associated virus encoding green fluorescent protein (AAV-GFP) was applied. For immunohistochemistry, Piezo2, ATF3, Caspase 3, ChAT and TDP-43 antibodies were used. At 1 month after tooth extraction, the number of Piezo2-immunoreactive (IR) Vmes neurons were decreased significantly. ATF3-IR neurons were detected on day 5 after tooth extraction. Dead cleaved caspase-3-IR neurons were found among Vmes neurons on days 7 and 12. In the Vmo, neuronal cytoplasmic inclusions (NCIs) formation type of TDP-43 increased at 1 and 2 months after extraction. These indicate the existence of neural projections from the Vmes to the periodontium in mice and that tooth loss induces the death of Vmes neurons followed by TDP-43 pathology in the Vmo. Therefore, tooth loss induces Vmes neuronal cell death, causing Vmo neurodegeneration and presumably affecting masticatory function.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 1","pages":"11-19"},"PeriodicalIF":2.4,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/0e/ahc-054-11.PMC7947638.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25488573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro. 紫外线b激活基质金属蛋白酶-2体外降解人纤毛带的研究

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-02-25 Epub Date: 2021-02-09 DOI: 10.1267/ahc.20-00021

Yuki Shiroto, Ryo Saga, Hironori Yoshino, Yoichiro Hosokawa, Keitaro Isokawa, Eichi Tsuruga

{"title":"Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro.","authors":"Yuki Shiroto, Ryo Saga, Hironori Yoshino, Yoichiro Hosokawa, Keitaro Isokawa, Eichi Tsuruga","doi":"10.1267/ahc.20-00021","DOIUrl":"https://doi.org/10.1267/ahc.20-00021","url":null,"abstract":"The ciliary zonules, also known as the zonules of Zinn, help to control the thickness of the lens during focusing. The ciliary zonules are composed of oxytalan fibers, which are synthesized by human nonpigmented ciliary epithelial cells (HNPCEC). The ciliary zonules are exposed to ultraviolet (UV), especially UV-A and UV-B, throughout life. We previously demonstrated that UV-B, but not UV-A, degrades fibrillin-1- and fibrillin-2-positive oxytalan fibers. However, the mechanism by which UV-B degrades oxytalan fibers remains unknown. In this study, we investigate the involvement of matrix metalloproteinase-2 (MMP-2) in the UV-B-induced degradation of fibrillin-1- and fibrillin-2-positive oxytalan fibers in cultured HNPCECs. Enzyme-linked immunosorbent assay revealed that UV-B irradiation at levels of 100 and 150 mJ/cm2 significantly increased the level of active MMP-2. Notably, MMP-2 inhibitors completely suppressed the degradation of fibrillin-1- and fibrillin-2-positive oxytalan fibers. In addition, we show that UV-B activates MMP-2 via stress-responsive kinase p38. Taken together, the results suggest that UV-B activates a production of active type of MMP-2 via the p38 pathway, and subsequently, an active-type MMP-2 degrades the fibrillin-1- and fibrillin-2-positive oxytalan fibers in cultured HNPCECs.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 1","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/68/ahc-054-1.PMC7947639.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25488572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2