Acta Histochemica Et Cytochemica最新文献_第8页

Development of Pathological Diagnosis Support System Using Micro-computed Tomography. 微计算机断层扫描病理诊断支持系统的研制。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-04-22 DOI: 10.1267/ahc.20-00033

Tomonari Hayakawa, Atsushi Teramoto, Yuka Kiriyama, Tetsuya Tsukamoto, Ayumi Yamada, Kuniaki Saito, Hiroshi Fujita

{"title":"Development of Pathological Diagnosis Support System Using Micro-computed Tomography.","authors":"Tomonari Hayakawa, Atsushi Teramoto, Yuka Kiriyama, Tetsuya Tsukamoto, Ayumi Yamada, Kuniaki Saito, Hiroshi Fujita","doi":"10.1267/ahc.20-00033","DOIUrl":"https://doi.org/10.1267/ahc.20-00033","url":null,"abstract":"In pathological diagnosis, the cutting position of pathological materials is subjectively determined by pathologists. This leads to a low cutting accuracy, which in turn may lead to incorrect diagnoses. In this study, we developed a system that supports the determination of the cutting position by visualizing and analyzing the internal structure of pathological material using micro-computed tomography (CT) before cutting. This system consists of a dedicated micro-CT and cutting support software. The micro-CT system has a fixture for fixing the target, enabling the scanning of easily deformable pathological materials. In the cutting support software, a function that interactively selects the extraction plane while displaying the volume rendering image and outputs a pseudo-histological image was implemented. Our results confirmed that the pseudo-histological image showed the fine structure inside the organ and that the latter image was highly consistent with the pathological image.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 2","pages":"49-56"},"PeriodicalIF":2.4,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/70/4c/ahc-054-49.PMC8116619.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38999689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia. 宫颈上皮内瘤变临床过程中HLAⅰ类免疫组织化学表达模式的改变。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-04-17 DOI: 10.1267/ahc.21-00010

Okuto Koguchi, Haruna Nishimaki, Yoko Nakanishi, Hiroko Kobayashi, Sumie Ohni, Xiaoyan Tang, Yoshiaki Kusumi, Shinobu Masuda

{"title":"Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia.","authors":"Okuto Koguchi, Haruna Nishimaki, Yoko Nakanishi, Hiroko Kobayashi, Sumie Ohni, Xiaoyan Tang, Yoshiaki Kusumi, Shinobu Masuda","doi":"10.1267/ahc.21-00010","DOIUrl":"https://doi.org/10.1267/ahc.21-00010","url":null,"abstract":"It is unclear how immunohistochemical expression patterns of HLA class I in the pre-malignant phase of cervical intraepithelial neoplasia (CIN) alter during the clinical follow-up period. The present study aimed to demonstrate the correlation between the immunohistochemical expression pattern of HLA class I and the CIN grade through repeated examinations during the clinical course. Expression patterns of HLA class I, p16INK4a, and PD-L1 were immunohistochemically examined using formalin-fixed paraffin-embedded (FFPE) sections of biopsy or conization samples that were obtained from 20 patients diagnosed with CIN. The mRNA expression levels of HLA class I were analyzed by real-time reverse transcription polymerase chain reaction using FFPE sections of 14 patients, who were examined metachronously during the follow-up period. HLA class I expression was limited to the lower part of the epithelial thickness (M1 pattern) in more than half of CIN1 cases, and was present throughout the epithelial thickness (M2 pattern) in one fourth of CIN1 and CIN2 cases approximately. Heterogeneous expression (H pattern) was detected in half of CIN2 and CIN3 cases and in the all of squamous cell carcinoma cases. Metachronous examinations revealed that these immunohistochemical patterns altered more frequently than the CIN grade. The rate of change of HLA class I mRNA expression level was higher in cases with a progressed immunohistochemical pattern compared to those with regressed immunohistochemical pattern. In conclusion, the immunohistochemical pattern of HLA class I expression is associated with the CIN grade, and it is alterable during the clinical course, especially in CIN2.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 2","pages":"57-64"},"PeriodicalIF":2.4,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/40/ahc-054-57.PMC8116618.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38999691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation with Fermented Barley Extract Prevents Mammary Epithelial Cell Invasion in an Early Breast Cancer Model. 在早期乳腺癌模型中补充发酵大麦提取物可防止乳腺上皮细胞侵袭。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-04-28 Epub Date: 2021-02-27 DOI: 10.1267/ahc.20-00029

Junji Itou, Akihiro Nakamura, Hideki Hokazono, Masakazu Toi

引用次数: 0

The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice. 牙周损伤诱导C57BL/6J小鼠三叉神经中脑核神经元细胞死亡和三叉神经运动核神经退行性变。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-02-25 Epub Date: 2021-02-16 DOI: 10.1267/ahc.20-00036

Ashis Dhar, Eriko Kuramoto, Makoto Fukushima, Haruki Iwai, Atsushi Yamanaka, Tetsuya Goto

{"title":"The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice.","authors":"Ashis Dhar, Eriko Kuramoto, Makoto Fukushima, Haruki Iwai, Atsushi Yamanaka, Tetsuya Goto","doi":"10.1267/ahc.20-00036","DOIUrl":"https://doi.org/10.1267/ahc.20-00036","url":null,"abstract":"Proprioception from masticatory apparatus and periodontal ligaments comes through the trigeminal mesencephalic nucleus (Vmes). We evaluated the effects of tooth loss on neurodegeneration of the Vmes and trigeminal motor nucleus (Vmo). Bilateral maxillary molars of 2-month-old C57BL/6J mice were extracted under anesthesia. Neural projections of the Vmes to the periodontium were confirmed by injecting Fluoro-Gold (FG) retrogradely into the extraction sockets, and for the anterograde labeling adeno-associated virus encoding green fluorescent protein (AAV-GFP) was applied. For immunohistochemistry, Piezo2, ATF3, Caspase 3, ChAT and TDP-43 antibodies were used. At 1 month after tooth extraction, the number of Piezo2-immunoreactive (IR) Vmes neurons were decreased significantly. ATF3-IR neurons were detected on day 5 after tooth extraction. Dead cleaved caspase-3-IR neurons were found among Vmes neurons on days 7 and 12. In the Vmo, neuronal cytoplasmic inclusions (NCIs) formation type of TDP-43 increased at 1 and 2 months after extraction. These indicate the existence of neural projections from the Vmes to the periodontium in mice and that tooth loss induces the death of Vmes neurons followed by TDP-43 pathology in the Vmo. Therefore, tooth loss induces Vmes neuronal cell death, causing Vmo neurodegeneration and presumably affecting masticatory function.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 1","pages":"11-19"},"PeriodicalIF":2.4,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/0e/ahc-054-11.PMC7947638.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25488573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro. 紫外线b激活基质金属蛋白酶-2体外降解人纤毛带的研究

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-02-25 Epub Date: 2021-02-09 DOI: 10.1267/ahc.20-00021

Yuki Shiroto, Ryo Saga, Hironori Yoshino, Yoichiro Hosokawa, Keitaro Isokawa, Eichi Tsuruga

{"title":"Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro.","authors":"Yuki Shiroto, Ryo Saga, Hironori Yoshino, Yoichiro Hosokawa, Keitaro Isokawa, Eichi Tsuruga","doi":"10.1267/ahc.20-00021","DOIUrl":"https://doi.org/10.1267/ahc.20-00021","url":null,"abstract":"The ciliary zonules, also known as the zonules of Zinn, help to control the thickness of the lens during focusing. The ciliary zonules are composed of oxytalan fibers, which are synthesized by human nonpigmented ciliary epithelial cells (HNPCEC). The ciliary zonules are exposed to ultraviolet (UV), especially UV-A and UV-B, throughout life. We previously demonstrated that UV-B, but not UV-A, degrades fibrillin-1- and fibrillin-2-positive oxytalan fibers. However, the mechanism by which UV-B degrades oxytalan fibers remains unknown. In this study, we investigate the involvement of matrix metalloproteinase-2 (MMP-2) in the UV-B-induced degradation of fibrillin-1- and fibrillin-2-positive oxytalan fibers in cultured HNPCECs. Enzyme-linked immunosorbent assay revealed that UV-B irradiation at levels of 100 and 150 mJ/cm2 significantly increased the level of active MMP-2. Notably, MMP-2 inhibitors completely suppressed the degradation of fibrillin-1- and fibrillin-2-positive oxytalan fibers. In addition, we show that UV-B activates MMP-2 via stress-responsive kinase p38. Taken together, the results suggest that UV-B activates a production of active type of MMP-2 via the p38 pathway, and subsequently, an active-type MMP-2 degrades the fibrillin-1- and fibrillin-2-positive oxytalan fibers in cultured HNPCECs.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 1","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/68/ahc-054-1.PMC7947639.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25488572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells. 胎盘霍夫鲍尔细胞光镜、ImageJ和QuPath免疫组化染色强度的比较分析。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-02-25 Epub Date: 2021-02-20 DOI: 10.1267/ahc.20-00032

Katerina Cizkova, Tereza Foltynkova, Mariam Gachechiladze, Zdenek Tauber

{"title":"Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells.","authors":"Katerina Cizkova, Tereza Foltynkova, Mariam Gachechiladze, Zdenek Tauber","doi":"10.1267/ahc.20-00032","DOIUrl":"https://doi.org/10.1267/ahc.20-00032","url":null,"abstract":"Software based analyses of immunohistochemical staining are designed for obtaining quantitative, reproducible, and objective data. However, often times only a certain type of positive cells or structures need to be quantified thus whole image analysis cannot be performed. Such an example is Hofbauer placental cells, which show positivity of some antigens together with trophoblast, but only Hofbauer cells represent the regions of interest (ROIs). Two independent observers evaluated the immunohistochemical staining intensity of Hofbauer cells in placenta samples stained for cytoplasmic antigens by ImageJ, QuPath and light microscopy. Thus, the precise manual determination of ROIs, i.e. Hofbauer cells, was necessary. We detected low inter-observer variability in staining intensity. Almost perfect agreement between observers was reached for ImageJ and QuPath whilst substantial agreement was reached for light microscopy evaluation. As for the comparison of ImageJ, QuPath and light microscopy, the agreement of all three methods (identical immunohistochemical intensity) was achieved for 38.1% samples. The almost perfect agreement of staining intensities was reached between ImageJ and QuPath, and moderate agreement for comparison of the light microscopy to both software. Software analyses are much more time-consuming, thus their utilization is at least questionable to evaluate ROIs with selection.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 1","pages":"21-29"},"PeriodicalIF":2.4,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/df/ahc-054-21.PMC7947637.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25488574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Minocycline Alleviates Cluster Formation of Activated Microglia and Age-dependent Dopaminergic Cell Death in the Substantia Nigra of Zitter Mutant Rat. 米诺环素减轻Zitter突变大鼠黑质活化小胶质细胞簇形成和年龄依赖性多巴胺能细胞死亡。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-12-25 Epub Date: 2020-11-21 DOI: 10.1267/ahc.20-00022

Daisuke Taguchi, Ayuka Ehara, Taro Kadowaki, Shin-Ichi Sakakibara, Kazuhiko Nakadate, Koichi Hirata, Shuichi Ueda

{"title":"Minocycline Alleviates Cluster Formation of Activated Microglia and Age-dependent Dopaminergic Cell Death in the Substantia Nigra of Zitter Mutant Rat.","authors":"Daisuke Taguchi, Ayuka Ehara, Taro Kadowaki, Shin-Ichi Sakakibara, Kazuhiko Nakadate, Koichi Hirata, Shuichi Ueda","doi":"10.1267/ahc.20-00022","DOIUrl":"https://doi.org/10.1267/ahc.20-00022","url":null,"abstract":"Microglial activation is a component of neurodegenerative pathology. Here, we examine whether activated microglia participate in age-related dopaminergic (DA) cell death in the substantia nigra pars compacta (SNc) of the zitter (zi/zi) rat, a mutant characterized by deletion of the attractin gene. Confocal microscopy with double-immunohistochemical staining revealed activated microglia-formed cell-clusters surrounding DA neurons in the SNc from 2 weeks after birth. An immunoelectron microscopic study showed that the cytoplasm of activated microglia usually contains phagosome-like vacuoles and lamellar inclusions. Expression levels of the pro-inflammatory cytokines interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in the midbrain of 2-month-old zi/zi rats. Chronic treatment with the anti-inflammatory agent minocycline altered the morphology of the microglia, reduced cluster formation by the microglia, and attenuated DA cell death in the SNc, and reduced the expression of IL-1β in the midbrain. These results indicate that activated microglia, at least in part and especially at the initial phase, contribute to DA cell death in the SNc of the zi/zi rat.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 6","pages":"139-146"},"PeriodicalIF":2.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38812593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mercury and Selenium Localization in the Cerebrum, Cerebellum, Liver, and Kidney of a Minamata Disease Case. 汞和硒在水俣病患者脑、小脑、肝和肾中的定位。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-12-25 Epub Date: 2020-12-19 DOI: 10.1267/ahc.20-00009

Masumi Marumoto, Mineshi Sakamoto, Kohji Marumoto, Shozo Tsuruta, Yoshihiro Komohara

{"title":"Mercury and Selenium Localization in the Cerebrum, Cerebellum, Liver, and Kidney of a Minamata Disease Case.","authors":"Masumi Marumoto, Mineshi Sakamoto, Kohji Marumoto, Shozo Tsuruta, Yoshihiro Komohara","doi":"10.1267/ahc.20-00009","DOIUrl":"https://doi.org/10.1267/ahc.20-00009","url":null,"abstract":"Minamata disease is a methylmercury poisoning caused by consumption of marine food contaminated by man-made methylmercury environmental pollution, and its most prominent feature is marked pathological changes in the central nervous system. Morphological alterations are less pronounced in the liver and the kidney, although their mercury levels are higher than those of the brain. In marine mammals, methylmercury is known to be easily converted to inorganic mercury and it combines with selenium forming mercury selenide, which may counteract the toxicity of mercury. However, little is known about the formation of mercury and selenium complex in human organs. In the present study, we examined the cerebrum, cerebellum, liver, and kidney of a Minamata disease case to study the mercury and selenium localization using electron probe microanalysis. Our results indicated the mercury and selenium localization in the specified tissue of the brain, liver, and kidney such as glial cells, Kupffer cells, and renal tubules.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 6","pages":"147-155"},"PeriodicalIF":2.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38812594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

High-quality Fluorescence Imaging of the Human Acrosyringium Using a Transparency: Enhancing Technique and an Improved, Fluorescent Solvatochromic Pyrene Probe. 利用透明增强技术和改进的荧光溶剂致变色芘探针对人肢端注射器进行高质量荧光成像。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-12-25 Epub Date: 2020-11-21 DOI: 10.1267/ahc.20-00020

Masamoto Murakami, Ryosuke Kawakami, Yosuke Niko, Teruko Tsuda, Hideki Mori, Kazuki Yatsuzuka, Takeshi Imamura, Koji Sayama

{"title":"High-quality Fluorescence Imaging of the Human Acrosyringium Using a Transparency: Enhancing Technique and an Improved, Fluorescent Solvatochromic Pyrene Probe.","authors":"Masamoto Murakami, Ryosuke Kawakami, Yosuke Niko, Teruko Tsuda, Hideki Mori, Kazuki Yatsuzuka, Takeshi Imamura, Koji Sayama","doi":"10.1267/ahc.20-00020","DOIUrl":"https://doi.org/10.1267/ahc.20-00020","url":null,"abstract":"Two-photon, excitation fluorescent microscopy featuring autofluorescence or immunofluorescence, combined with optical clearance using a transparency-enhancing technique, allows deep imaging of three-dimensional (3D) skin structures. However, it remains difficult to obtain high-quality images of individual cells or 3D structures. We combined a new dye with a transparency-enhancing technology and performed high-quality structural analysis of human epidermal structures, especially the acrosyringium. Human fingertip skin samples were collected, formalin-fixed, embedded in both frozen and paraffin blocks, sliced, stained with propidium iodide, optically cleared using a transparency-enhancing technique, and stained with a new fluorescent, solvatochromic pyrene probe. Microscopy revealed fine skin features and detailed epidermal structures including the stratum corneum (horny layer), keratinocytes, eccrine sweat glands, and peripheral nerves. Three-dimensional reconstruction of an entire acrosyringium was possible in one sample. This new fluorescence microscopy technique yields high-quality epidermal images and will aid in histopathological analyses of skin disorders.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 6","pages":"131-138"},"PeriodicalIF":2.4,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38812592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Loss of Annexin A10 Expression Is Associated with Poor Prognosis in Early Gastric Cancer. 早期胃癌患者Annexin A10表达缺失与预后不良有关

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2020-10-29 Epub Date: 2020-09-24 DOI: 10.1267/ahc.20-00014

Akira Ishikawa, Kazuya Kuraoka, Junichi Zaitsu, Akihisa Saito, Toshio Kuwai, Takahisa Suzuki, Hirotaka Tashiro, Kiyomi Taniyama, Wataru Yasui

{"title":"Loss of Annexin A10 Expression Is Associated with Poor Prognosis in Early Gastric Cancer.","authors":"Akira Ishikawa, Kazuya Kuraoka, Junichi Zaitsu, Akihisa Saito, Toshio Kuwai, Takahisa Suzuki, Hirotaka Tashiro, Kiyomi Taniyama, Wataru Yasui","doi":"10.1267/ahc.20-00014","DOIUrl":"10.1267/ahc.20-00014","url":null,"abstract":"Gastric cancer (GC) is the third most common cause of cancer-related mortality. The diagnosis and treatment of early GC is a crucial strategy for prognostic improvement of GC. Annexin A10 (ANXA10), a calcium-/phospholipid-binding protein, is a member of the annexin family. The significance of ANXA10 expression in early GC remains unclear. This is the first report to investigate ANXA10 expression in early GC. We performed immunohistochemistry to evaluate ANXA10 expression in early GC, and the correlation between ANXA10 and clinicopathological factors. The loss of ANXA10 expression was detected in 63 (61.2%) of 103 early GC cases and significantly correlated with poor overall survival in patients. Sex, pT stage, pN stage, histology, and ANXA10 expression were associated with poor survival. Sex, histology, and ANXA10 expression were determined as independent predictors of survival in early GC patients. ANXA10 immunostaining could be a new decision-making biomarker in GC.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"53 5","pages":"113-119"},"PeriodicalIF":1.6,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38591135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0