Acta Histochemica Et Cytochemica最新文献_第7页

Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats. 水飞蓟素对败血症模型大鼠肝脏的保护作用

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-02-28 Epub Date: 2023-02-25 DOI: 10.1267/ahc.22-00059

Nevra Aydemir Celep, Semin Gedikli

{"title":"Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats.","authors":"Nevra Aydemir Celep, Semin Gedikli","doi":"10.1267/ahc.22-00059","DOIUrl":"10.1267/ahc.22-00059","url":null,"abstract":"This study, it was investigated whether silymarin has a protective effect by performing histological, immunohistochemical, and biochemical evaluations on the liver damage induced by cecal ligation perforation (CLP). CLP model was established and silymarin was treated at a dose of 50 mg/kg, 100 mg/kg, and 200 mg/kg, by oral one hour before the CLP. As an effect of the histological evaluations of the liver tissues, venous congestion, inflammation, and necrosis in the hepatocytes were observed in the CLP group. A situation close to the control group was observed in the Silymarin (SM)100 and SM200 groups. As a result of the immunohistochemical evaluations, inducible nitric oxide synthase (iNOS), cytokeratine (CK)18, Tumor necrosis factor-alpha (TNF-α), and interleukine (IL)-6 immunoreactivities were intense in the CLP group. In the biochemical analysis, Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) levels were significantly increased in the CLP group, while a significant decrease was observed in the treatment groups. TNFα, IL-1β, and IL-6 concentrations were in parallel with histopathological evaluations. In the biochemical analysis, Malondialdehyte (MDA) level increased significantly in the CLP group, but there was a significant decrease in the SM100 and SM200 groups. Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxidase (GSH-Px) activities were relatively low in the CLP group. According to these data, it was concluded that using silymarin reduces the existing liver damage in sepsis.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"56 1","pages":"9-19"},"PeriodicalIF":1.6,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/15/46/ahc-56-9.PMC9986308.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9137234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cathepsin D Inhibits Angiogenesis in Pituitary Neuroendocrine Tumors. 组织蛋白酶D抑制垂体神经内分泌肿瘤血管生成。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-12-28 DOI: 10.1267/ahc.22-00098

Ren Fujiwara, Hirotomo Ten, Hui Chen, Chuan-Lu Jiang, Ken-Ichi Oyama, Keisuke Onoda, Akira Matsuno

{"title":"Cathepsin D Inhibits Angiogenesis in Pituitary Neuroendocrine Tumors.","authors":"Ren Fujiwara, Hirotomo Ten, Hui Chen, Chuan-Lu Jiang, Ken-Ichi Oyama, Keisuke Onoda, Akira Matsuno","doi":"10.1267/ahc.22-00098","DOIUrl":"https://doi.org/10.1267/ahc.22-00098","url":null,"abstract":"Prolactin and growth hormone can acquire anti-angiogenic properties after undergoing proteolytic cleavage by Cathepsin D and bone morphogenetic protein 1 (BMP-1) into fragments known as vasoinhibins. Little is known about the effect of vasoinhibins on angiogenesis through the involvement of key cleavage enzymes Cathepsin D and BMP-1 in pituitary neuroendocrine tumors (PitNETs, formerly pituitary adenomas). The purpose of this study was to investigate the mechanism of action of Cathepsin D and BMP-1 on angiogenesis in PitNETs compared with that of pro-angiogenic factors, including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor-2 (FGF2). A total of 43 patients were enrolled in a retrospective analysis and 22 samples were suitable for RNA extraction, including 16 nonfunctional PitNETs and six somatotroph tumors. The mRNA and protein levels of Cathepsin D, BMP-1, VEGF, and FGF2 were compared with those of von Willebrand factor, which was assessed to determine the vascularization of PitNETs. Cathepsin D and FGF2 were significantly correlated with vascularization in PitNETs. Both Cathepsin D and FGF2 are highly involved in angiogenesis in PitNETs, although the effect of Cathepsin D as an anti-angiogenic factor is dominant over that of FGF2 as a pro-angiogenic factor.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 6","pages":"203-211"},"PeriodicalIF":2.4,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a7/a9/ahc-055-203.PMC9840469.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10607111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe [Acta Histochem. Cytochem. 55, 119-128 (2022)]. 勘误:使用基于荧光共振能量转移的分子信标探针进行原位杂交的先进检测系统[组织化学学报]。中国生物医学工程学报，2016,33(2):444 - 444。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-12-28 DOI: 10.1267/ahc.22-00110E

引用次数: 0

Low Doses of Bisphenol A Disrupt Neuronal Differentiation of Human Neuronal Stem/Progenitor Cells. 低剂量双酚A破坏人神经干/祖细胞的神经分化

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-12-28 DOI: 10.1267/ahc.22-00090

Kaori Kiso-Farnè, Takeshi Yaoi, Takahiro Fujimoto, Kyoko Itoh

{"title":"Low Doses of Bisphenol A Disrupt Neuronal Differentiation of Human Neuronal Stem/Progenitor Cells.","authors":"Kaori Kiso-Farnè, Takeshi Yaoi, Takahiro Fujimoto, Kyoko Itoh","doi":"10.1267/ahc.22-00090","DOIUrl":"https://doi.org/10.1267/ahc.22-00090","url":null,"abstract":"Bisphenol A (BPA) is an endocrine disrupting chemical. Human epidemiological studies have suggested that adverse neurobehavioral outcomes are induced by fetal exposure to BPA. The remarkable differences in the corticogenesis between human and agyrencephalic mammals are an increase in the intermediate progenitor cells (IPCs) and a following increase in the subplate thickness. It is uncertain whether low doses of BPA (low-BPA) affect human early corticogenesis when basal progenitor cells (BPs) produce IPCs resulting in amplified neurogenesis. In this study, human-derived neuronal stem/progenitor cells were exposed to low-BPA or the vehicle only, and the resultant cell type-specific molecular changes and morphology were analyzed. We focused on stem cells immunoreactive for SOX2, BPs for NHLH1, and immature neurons for DCX. SOX2-positive cells significantly decreased at day in vitro (DIV) 4 and 7, whereas NHLH1-positive cells tended to be higher, while DCX-positive cells significantly increased at DIV7 when exposed to 100 nM of BPA compared with the vehicle. Morphologically DCX-positive cells showed a decrease in unipolar cells and an increase in multipolar cells when exposed to 100 nM of BPA compared with the vehicle. These results provide insights into the in vivo effect of low-BPA on neuronal differentiation in the human fetal corticogenesis.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 6","pages":"193-202"},"PeriodicalIF":2.4,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d3/42/ahc-055-193.PMC9840471.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9176486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Localization of Alogliptin, a DPP-4 Inhibitor, in Tissues of Normal and Type 2 Diabetes Model Rat. DPP-4抑制剂阿格列汀在正常和2型糖尿病模型大鼠组织中的免疫组织化学定位

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-12-28 DOI: 10.1267/ahc.22-00032

Yutaro Yamamoto, Kanae Ura, Takuma Matsukawa, Tetsuya Saita, Masashi Shin

{"title":"Immunohistochemical Localization of Alogliptin, a DPP-4 Inhibitor, in Tissues of Normal and Type 2 Diabetes Model Rat.","authors":"Yutaro Yamamoto, Kanae Ura, Takuma Matsukawa, Tetsuya Saita, Masashi Shin","doi":"10.1267/ahc.22-00032","DOIUrl":"https://doi.org/10.1267/ahc.22-00032","url":null,"abstract":"We investigated the pharmacokinetics of alogliptin (AG) at the cell and tissue level in healthy Wistar rats and a type 2 diabetic Goto-Kakizaki (GK) rat model. Immunohistochemistry of the renal tissue in these rats, post 1 hr of AG administration, showed that the signal was observed in the glomeruli, proximal tubule S3 segments, distal tubules, collecting ducts, and only in the brush border of the epithelial cells of the proximal tubule S1, S2 segments. After 6 hr of AG administration, the staining intensity of the regions other than the S3 segments was considerably reduced in Wistar rats, with no change observed in GK rats. At 24 hr, the staining intensity was considerably reduced, even in GK rats; however, the staining of the S3 segment remained unaltered in both. Hepatocytes in zone III of the hepatic lobule were more intensely stained than those in zone I in Wistar rats at 1 hr. However, almost no staining was observed in the hepatocytes of GK rats at 1 hr. Complete loss of signal was observed in the hepatocytes of the Wistar rats after 6 hr. This study revealed that the pharmacokinetics of AG in GK rats are different from those in Wistar rats.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 6","pages":"185-192"},"PeriodicalIF":2.4,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/80/ahc-055-185.PMC9840470.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10661773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Cancer Stem-like Cells in the Process of Invasion and Mesenchymal Transformation by a Reconstituted Triple-negative Breast Cancer Cell Population Resistant to p53-induced Apoptosis. 肿瘤干细胞样细胞在抗p53诱导凋亡重组三阴性乳腺癌细胞群侵袭和间充质转化过程中的作用

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00076

Sana Inoue, Miki Imanishi, Ai Kanzaki, Atsumi Fujimoto, Marina Maeyama, Ayaka Okamoto, Hiroka Matsuda, Kiyotsugu Yoshikawa, Rei Takahashi

{"title":"Role of Cancer Stem-like Cells in the Process of Invasion and Mesenchymal Transformation by a Reconstituted Triple-negative Breast Cancer Cell Population Resistant to p53-induced Apoptosis.","authors":"Sana Inoue, Miki Imanishi, Ai Kanzaki, Atsumi Fujimoto, Marina Maeyama, Ayaka Okamoto, Hiroka Matsuda, Kiyotsugu Yoshikawa, Rei Takahashi","doi":"10.1267/ahc.22-00076","DOIUrl":"https://doi.org/10.1267/ahc.22-00076","url":null,"abstract":"We investigated the role of cancer stem cells (CSCs) in a population of triple-negative breast cancer (TNBC) cells that are resistant to apoptosis. A human breast cancer cell population capable of inducing p53 expression with doxycycline (Dox) was created and used as an untreated control (UT). After the addition of Dox to UT for 5 days, the cell population reconstituted with cells showing resistance to apoptosis was named RE. Fluorescence-activated cell sorting (FACS) and immunostaining revealed that after the addition of Dox, the ratio of cells in the S and G2/M phases decreased in UT as apoptosis proceeded, but did not markedly change in apoptosis-resistant RE. CSC-like cells in RE exhibited a cell morphology with a larger ratio of the major/minor axis than UT. FACS showed that RE had a higher proportion of CSC-like cells and contained more CD44+CD24- mesenchymal CSCs than ALDH1A3+ epithelial-like CSCs. In a Matrigel invasion assay, UT was more likely to form a three-dimensional cell population, whereas RE exhibited a planar population, higher migration ability, and the up-regulated expression of epithelial-mesenchymal transition-related genes. These results provide insights into the mechanisms by which TNBC cells acquire treatment resistance at the time of recurrence.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":" ","pages":"169-184"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/c2/ahc-055-169.PMC9631983.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40697213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody. PPZ0506单克隆抗体优化小鼠ESR2蛋白的小鼠对小鼠免疫组化检测

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00043

Mina Ozawa, Yujiro Hattori, Shimpei Higo, Mai Otsuka, Keisuke Matsumoto, Hitoshi Ozawa, Hirotaka Ishii

{"title":"Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody.","authors":"Mina Ozawa, Yujiro Hattori, Shimpei Higo, Mai Otsuka, Keisuke Matsumoto, Hitoshi Ozawa, Hirotaka Ishii","doi":"10.1267/ahc.22-00043","DOIUrl":"https://doi.org/10.1267/ahc.22-00043","url":null,"abstract":"Despite the physiological significance of ESR2, a lack of well-validated detection systems for ESR2 proteins has hindered progress in ESR2 research. Thus, recent identification of a specific anti-human ESR2 monoclonal antibody (PPZ0506) and its specific cross-reactivity against mouse and rat ESR2 proteins heightened momenta toward development of appropriate immunohistochemical detection systems for rodent ESR2 proteins. Building upon our previous optimization of ESR2 immunohistochemical detection in rats using PPZ0506, in this study, we further aimed to optimize mouse-on-mouse immunohistochemical detection using PPZ0506. Our assessment of several staining conditions using paraffin-embedded ovary sections revealed that intense heat-induced antigen retrieval, appropriate blocking, and appropriate antibody dilutions were necessary for optimization of mouse-on-mouse immunohistochemistry. Subsequently, we applied the optimized immunostaining method to determine expression profiles of mouse ESR2 proteins in peripheral tissues and brain subregions. Our analyses revealed more localized distribution of mouse ESR2 proteins than previously assumed. Moreover, comparison of these results with those obtained in humans and rats using PPZ0506 revealed interspecies differences in ESR2 expression. We expect that our optimized methodology for immunohistochemical staining of mouse ESR2 proteins will help researchers to solve multiple lines of controversial evidence concerning ESR2 expression.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":" ","pages":"159-168"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1e/6b/ahc-055-159.PMC9631985.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40698206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe. 基于荧光共振能量转移分子信标探针的原位杂交先进检测系统。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 DOI: 10.1267/ahc.22-00075

Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa

{"title":"An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe.","authors":"Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa","doi":"10.1267/ahc.22-00075","DOIUrl":"https://doi.org/10.1267/ahc.22-00075","url":null,"abstract":"In situ hybridization (ISH) is a powerful method for detecting specific RNAs at the cellular level. Although conventional ISH using hapten-labeled probes are useful for detecting multiple RNAs, the detection procedures are still complex and required longer time. Therefore, we introduced a new application of fluorescence resonance energy transfer (FRET)-based molecular beacon (MB) probes for ISH. MCF-7 cells and C57BL/6J mouse uterus were used for ISH. MB probes for ERα mRNA and 28S rRNA were labeled with Cy3/BHQ-2 and 6-FAM/DABCYL, and conventional probes were labeled with digoxigenin. Fluorescence measurements revealed that of more-rapid hybridization kinetics compared to conventional probes. In MCF-7 cells, 28S rRNA was detected in nucleolus and cytoplasm of all cells, whereas ERα mRNA was detected in some nucleolus. In the uterus, 28S rRNA was clearly detected using complementary MB probe, but there were no signals in control slides. Moreover, 28S rRNA was detected in all cells, whereas ERα mRNA was detected mainly in the epithelium. Fluorescence intensity of 28S rRNA was decreased significantly in 1 or 2 base-mismatched sequences, that indicates highly specific detection of target RNAs. In conclusion, the FRET-based MB probes are very useful for ISH, providing rapid hybridization, high sensitivity and specificity.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 5","pages":"119-128"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ae/9a/ahc-055-119.PMC9631986.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9108416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding. 酶标抗原法:影响福尔马林固定和石蜡包埋过程中特异性抗体抗原结合活性下降的因素。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00023

Yasuyoshi Mizutani, Kazuya Shiogama, Ken-Ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi

{"title":"Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding.","authors":"Yasuyoshi Mizutani, Kazuya Shiogama, Ken-Ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi","doi":"10.1267/ahc.22-00023","DOIUrl":"https://doi.org/10.1267/ahc.22-00023","url":null,"abstract":"The enzyme-labeled antigen method is an immunohistochemical technique detecting plasma cells producing specific antibodies in tissue sections. The probe is an antigen labeled with an enzyme or biotin. This immunohistochemical technique is appliable to frozen sections of paraformaldehyde (PFA)-fixed tissues, but it has been difficult to apply it to formalin-fixed, paraffin-embedded (FFPE) sections. In the current study, factors inactivating the antibody reactivity during the process of preparing FFPE sections were investigated. Lymph nodes of rats immunized with horseradish peroxidase (HRP) or a mixture of keyhole limpet hemocyanin/ovalbumin/bovine serum albumin were employed as experimental models. Plasma cells producing specific antibodies, visualized with HRP (as an antigen with enzymatic activity) or biotinylated proteins in 4% PFA-fixed frozen sections, significantly decreased in unbuffered 10% formalin-fixed frozen sections. The positive cells were further decreased by paraffin embedding following formalin fixation. In paraffin-embedded sections fixed in precipitating fixatives such as ethanol and acetone and those prepared with the AMeX method, the antigen-binding reactivity of antibodies was preserved. Fixation in periodate-lysine-paraformaldehyde and Zamboni solution also kept the antigen-binding reactivity in paraffin to some extent. In conclusion, formalin fixation and paraffin embedding were major causes inactivating antibodies. Precipitating fixatives could retain the antigen-binding reactivity of antibodies in paraffin-embedded sections.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":" ","pages":"129-148"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/3b/ahc-055-129.PMC9631987.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40698207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats. 艾布selen对大鼠上腹部皮瓣缺血再灌注损伤的保护作用。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00062

Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu

{"title":"Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats.","authors":"Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu","doi":"10.1267/ahc.22-00062","DOIUrl":"https://doi.org/10.1267/ahc.22-00062","url":null,"abstract":"The purpose of this study was to determine the role of oxidized diacylglycerol (DAG) and the molecular mechanism underlying ischemia-reperfusion (I/R) injury in rat skin flaps. The protective effect of ebselen on the viability of rat skin flaps with I/R injury was investigated. Flaps were designed and raised in the left inguinal region. Then, a microvascular clamp was applied to the vascular pedicle and reperfused after 6 hr. After 7 days of I/R (I/R group), the skin flap survival area ratio was significantly reduced compared to the normal skin. The administration of ebselen significantly improved the ratio compared to the I/R group. The flap survival area ratio of the I/R + ebselen group was significantly improved compared to the I/R + vehicle group. In the I/R + ebselen group, the oxidized DAG content and intensity of phosphorylated PKCα and PKCδ were significantly lower compared to the I/R + vehicle group. Furthermore, the inflammatory response was suppressed in the I/R + ebselen group compared to the I/R + vehicle group. These results indicate that ebselen is useful as a preventive and therapeutic agent for skin flap necrosis caused by I/R, because of reduction and elimination of oxidized DAG.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":" ","pages":"149-157"},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/c2/ahc-055-149.PMC9631984.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40697214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0