Acta Histochemica Et Cytochemica最新文献_第7页

Immunolocalization of CD80 and CD86 in Non-Small Cell Lung Carcinoma: CD80 as a Potent Prognostic Factor CD80和CD86在非小细胞肺癌中的免疫定位:CD80是一个有效的预后因素

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-02-11 DOI: 10.1267/ahc.21-00075

Takashi Sato, K. Takagi, M. Higuchi, H. Abe, Michie Kojimahara, Miho Sagawa, Megumi Tanaki, Y. Miki, Takashi Suzuki, H. Hojo

{"title":"Immunolocalization of CD80 and CD86 in Non-Small Cell Lung Carcinoma: CD80 as a Potent Prognostic Factor","authors":"Takashi Sato, K. Takagi, M. Higuchi, H. Abe, Michie Kojimahara, Miho Sagawa, Megumi Tanaki, Y. Miki, Takashi Suzuki, H. Hojo","doi":"10.1267/ahc.21-00075","DOIUrl":"https://doi.org/10.1267/ahc.21-00075","url":null,"abstract":"It has been demonstrated that tumor cells express programed cell death protein 1 (PD-L1) to escape T lymphocytes that express programed cell protein 1 (PD-1), and PD-1/PD-L1 immune checkpoint inhibitors have been regarded in lung cancer patients. CD80 and CD86 are members of B7 superfamily which regulates T lymphocyte activation and tolerance. However, immunolocalization of CD80 and CD86 has not been examined in the lung carcinoma tissues and their clinical significance remains unknown. Therefore, to clarify clinical significance of CD80 and CD86, we immunolocalized these in 75 non-small cell lung carcinomas (NSCLC) in this study. Immunoreactivities of CD80 and CD86 were mainly detected in tumor-infiltrating macrophages. Immunohistochemical CD80 status was high in 56% of NSCLC, and it was positively associated with stage, pathological T factor, distant metastasis, histological type and PD-L1 status. Moreover, multivariate analysis turned out that the CD80 status was an independent worse prognostic factor. CD86 status was high in 53% of the cases, but it was not significantly associated with any clinicopathological parameters. These findings suggest that CD80 is a potent worse prognostic factor possibly in association with escape from immune attack in NSCLC.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 1","pages":"25 - 35"},"PeriodicalIF":2.4,"publicationDate":"2022-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42794811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Histochemistry, Cytochemistry and Epigenetics 组织化学、细胞化学和表观遗传学

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-02-11 DOI: 10.1267/ahc.21-00095

S. Kitazawa, Teruyuki Ohno, Ryuma Haraguchi, R. Kitazawa

{"title":"Histochemistry, Cytochemistry and Epigenetics","authors":"S. Kitazawa, Teruyuki Ohno, Ryuma Haraguchi, R. Kitazawa","doi":"10.1267/ahc.21-00095","DOIUrl":"https://doi.org/10.1267/ahc.21-00095","url":null,"abstract":"Over the past few decades, many researchers have individually identified tumor-related genes, and have accumulated information on their basic research in a database. With the development of technology that can comprehensively test the expression status within a short time, oncogene panel testing has become attainable. On the other hand, changes in gene expression that do not depend on changes in base sequences, that is, epigenetics, or more comprehensively, epigenomes, are also highly involved in the development and progression of disease. Oncogene panel tests tend to focus on DNA base mutations such as point mutations, deletions, duplications, and chimera formation. Elucidation leads to correct interpretation of diseases and treatment choices, and we are in an era where integrated understanding of the genome and epigenome is indispensable. In this review, we make every effort to cover a wide range of knowledge, including data on histone protein modification, non-coding (nc)RNA and DNA methylation, and recent application trials for demonstrating epigenetic alterations in histologic and cytologic specimens. We hope this review will help marshal the knowledge accumulated by researchers involved in genomic and epigenomic studies.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"55 1","pages":"1 - 7"},"PeriodicalIF":2.4,"publicationDate":"2022-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44795507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Differences in Regression Patterns of Complete and Incomplete Intestinal Metaplasia at Ten Years after Helicobacter pylori Eradication. 根除幽门螺旋杆菌十年后完全性和不完全性肠化生的回归模式差异。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-12-24 Epub Date: 2021-11-18 DOI: 10.1267/ahc.21-00069

Yasuhiro Wada, Masaaki Kodama, Kazuhiro Mizukami, Tadayoshi Okimoto, Takafumi Fuchino, Koshiro Tsutsumi, Masahide Fukuda, Yuka Hirashita, Kensuke Fukuda, Kazuhisa Okamoto, Ryo Ogawa, Ryoji Kushima, Kazunari Murakami

{"title":"Differences in Regression Patterns of Complete and Incomplete Intestinal Metaplasia at Ten Years after Helicobacter pylori Eradication.","authors":"Yasuhiro Wada, Masaaki Kodama, Kazuhiro Mizukami, Tadayoshi Okimoto, Takafumi Fuchino, Koshiro Tsutsumi, Masahide Fukuda, Yuka Hirashita, Kensuke Fukuda, Kazuhisa Okamoto, Ryo Ogawa, Ryoji Kushima, Kazunari Murakami","doi":"10.1267/ahc.21-00069","DOIUrl":"10.1267/ahc.21-00069","url":null,"abstract":"This study was conducted to reveal the reversibility of subtype of intestinal metaplasia (IM) and Paneth cells after H. pylori eradication (HPE). Among 75 patients, we retrospectively examined the proportions of patients with complete type of IM (CIM), incomplete type of IM (IIM) and Paneth cells in their biopsy specimens obtained from the greater curvature of the antrum (A2) and the greater curvature of the middle corpus (B2) before and during a follow-up period of 10 years after HPE. Immunohistochemistry was used to determine IM type. Compared to before HPE, the proportion of patients with CIM did not decrease significantly during the 10-year follow-up after HPE both in A2 (32% vs. 21.3%, P = 0.13) and in B2 (6.7% vs. 2.7%, P = 0.60). IIM rates in A2 was significantly lower during this time (26.7% vs. 10.7%, P = 0.04), whereas no patients showed IIM in B2 before HPE. The proportion of patients with Paneth cells decreased significantly in A2 after 3, 8, and 9 years of HPE and in B2 after 4, 6 and 9 years of HPE (P < 0.05 for all). Thus, IIM and Paneth cells regressed during a period of 10 years after HPE.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 6","pages":"185-194"},"PeriodicalIF":2.4,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/92/21-00069.PMC8727845.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39693210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear Expression of Pygo2 Correlates with Poorly Differentiated State Involving c-Myc, PCNA and Bcl9 in Myanmar Hepatocellular Carcinoma. 缅甸肝细胞癌中Pygo2的核表达与c-Myc、PCNA和Bcl9低分化状态相关

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-12-24 Epub Date: 2021-12-21 DOI: 10.1267/ahc.21-00090

Myo Win Htun, Yasuaki Shibata, Kyaw Soe, Takehiko Koji

{"title":"Nuclear Expression of Pygo2 Correlates with Poorly Differentiated State Involving c-Myc, PCNA and Bcl9 in Myanmar Hepatocellular Carcinoma.","authors":"Myo Win Htun, Yasuaki Shibata, Kyaw Soe, Takehiko Koji","doi":"10.1267/ahc.21-00090","DOIUrl":"https://doi.org/10.1267/ahc.21-00090","url":null,"abstract":"In Myanmar, hepatocellular carcinoma (HCC) is commonly seen in young adult and associated with poor prognosis, while the molecular mechanisms that characterize HCC in Myanmar are unknown. As co-activation of Wnt/β-catenin signaling and c-Myc (Myc) are reported to associate with malignancy of HCC, we immunohistochemically investigated the expression of Pygo2 and Bcl9, the co-activators of the Wnt/β-catenin signaling, Myc and PCNA in 60 cases of Myanmar HCC. Pygo2 expression was confirmed by in situ hybridization. The signal intensity was measured by image analyzer and then statistically analyzed. As a result, the expression of Pygo2 was significantly higher in HCC compared to normal liver tissue and the nuclear signal was the most intense in poorly differentiated HCC. Cytoplasmic Bcl9 was expressed in the normal liver tissue but decreased in HCC with the progression of histopathological grade. Myc was significantly higher in poorly differentiated HCC, whereas PCNA labeling index increased with the progression of histopathological grade. Nuclear Pygo2 showed strong correlation with nuclear Myc (P < 0.01) and PCNA (P < 0.001), and inversely correlated with cytoplasmic Bcl9 (P < 0.01). Our results suggested Wnt/β-catenin and Myc signaling is commonly activated in Myanmar HCC and that the correlative upregulation of nuclear Pygo2 and Myc characterizes the malignant features of HCC in Myanmar.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 6","pages":"195-206"},"PeriodicalIF":2.4,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/30/37/21-00090.PMC8727843.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39693211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination. 乳腺癌中蛋白-蛋白相互作用的可视化:病理检查中的部署研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-12-24 Epub Date: 2021-11-18 DOI: 10.1267/ahc.21-00084

Erina Iwabuchi, Yasuhiro Miki, Hironobu Sasano

{"title":"The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination.","authors":"Erina Iwabuchi, Yasuhiro Miki, Hironobu Sasano","doi":"10.1267/ahc.21-00084","DOIUrl":"https://doi.org/10.1267/ahc.21-00084","url":null,"abstract":"The therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore, it is important to focus on PPIs. This review summarizes the PPIs of ER and HER2 in breast cancer, especially those using a proximity ligation assay that can visualize PPIs in FFPE tissues. In particular, assessing the interaction of CEACAM6 with HER2 may serve as a surrogate marker for the efficacy of trastuzumab in patients with breast cancer. Therefore, in this review, the technique used to detect the interaction of CEACAM6 and HER2 in routinely processed pathological specimens will be applied to the clinical practice of drug selection. We showed the possibility as a novel pathological examination method using PPIs.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 6","pages":"177-183"},"PeriodicalIF":2.4,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/8c/21-00084.PMC8727844.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39693208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ZMYM3 May Promote Cell Proliferation in Small Cell Lung Carcinoma. ZMYM3可能促进小细胞肺癌细胞增殖。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-10-29 Epub Date: 2021-10-02 DOI: 10.1267/ahc.21-00012

Noritaka Kudo, Shinji Kudoh, Akira Matsuo, Yamato Motooka, Takaaki Ito

引用次数: 0

Tissue-specific Physical and Biological Microenvironments Modulate the Behavior of Cervical Squamous Cell Carcinoma. 组织特异性物理和生物微环境调节宫颈鳞状细胞癌的行为。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-10-29 Epub Date: 2021-10-06 DOI: 10.1267/ahc.21-00038

Katsuyuki Hanashima, Takashi Akutagawa, Mihoko Yamamoto-Rikitake, Takehisa Sakumoto, Maki Futamata, Yoshifumi Nakao, Masatoshi Yokoyama, Shuji Toda, Shigehisa Aoki

{"title":"Tissue-specific Physical and Biological Microenvironments Modulate the Behavior of Cervical Squamous Cell Carcinoma.","authors":"Katsuyuki Hanashima, Takashi Akutagawa, Mihoko Yamamoto-Rikitake, Takehisa Sakumoto, Maki Futamata, Yoshifumi Nakao, Masatoshi Yokoyama, Shuji Toda, Shigehisa Aoki","doi":"10.1267/ahc.21-00038","DOIUrl":"https://doi.org/10.1267/ahc.21-00038","url":null,"abstract":"The mechanisms controlling the aggressiveness and survival of cervical SCC cells remain unclear. We investigated how the physical and biological microenvironments regulate the growth, apoptosis and invasiveness of cervical cancer cells. Dynamic flow and air exposure were evaluated as physical microenvironmental factors, and stromal fibroblasts were evaluated as a biological microenvironmental factor. To investigate any regulatory effects of these microenvironmental factors, we established a new culture model which concurrently replicates fluid streaming, air exposure and cancer-stromal interactions. Three cervical cancer cell lines were cultured with or without NIH 3T3 fibroblasts. Air exposure was realized using a double-dish culture system. Dynamic flow was created using a rotary shaker. Dynamic flow and air exposure promoted the proliferative activity and decreased the apoptosis of cervical cancer cells. Fibroblasts regulated the invasive ability, growth and apoptosis of cervical cancer cells. Extracellular signal-regulated kinase and p38 signaling were regulated either synergistically or independently by dynamic flow, air exposure and cellular interactions, depending on the cervical cancer cell type. This study demonstrates that the physical and biological microenvironments interact to regulate the aggressiveness and survival of cervical cancer cells. Our simple culture system is a promising model for developing further treatment strategies for various types of cancer.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 5","pages":"155-165"},"PeriodicalIF":2.4,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d7/ad/21-00038.PMC8569132.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39612330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cold-induced Conversion of Connective Tissue Skeleton in Brown Adipose Tissues. 棕色脂肪组织结缔组织骨架的冷诱导转化。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-10-29 Epub Date: 2021-10-06 DOI: 10.1267/ahc.21-00030

Masako Yudasaka, Yuko Okamatsu-Ogura, Takeshi Tanaka, Kumiko Saeki, Hiromichi Kataura

{"title":"Cold-induced Conversion of Connective Tissue Skeleton in Brown Adipose Tissues.","authors":"Masako Yudasaka, Yuko Okamatsu-Ogura, Takeshi Tanaka, Kumiko Saeki, Hiromichi Kataura","doi":"10.1267/ahc.21-00030","DOIUrl":"https://doi.org/10.1267/ahc.21-00030","url":null,"abstract":"Thermogenesis via fatty acid-induced uncoupled mitochondrial respiration is the primary function of brown adipose tissue (BAT). In response to changes in ambient temperatures, the weight and specific gravity of BAT change, depending on the quantity of lipid droplets stored in brown adipocytes (BA). Such conditions should result in the reconstruction of connective tissue skeletons, especially of collagen fiber networks, although the mechanisms have not been clarified. This study showed that, within 4 hr of exposing mice to a cold environment, collagen fibers in the extracellular matrix (ECM) of BAT became discontinuous, twisted, emancipated, and curtailed. Surprisingly, the structure of collagen fibers returned to normal after the mice were kept at room temperature for 19 hr, indicating that the alterations in collagen fiber structures are physiological processes association with adaptation to cold environments. These dynamic changes in connective tissue skeletons were not observed in white adipose tissues, suggesting that they are unique to BAT. Interestingly, the vascular permeability of BAT was also augmented by exposure to cold. Collectively, these findings indicate that dynamic changes in ECM collagen fibers provide high flexibility to BAT, enabling the adjustment of tissue structures and the regulation of vascular permeability, resulting in adaptation to changes in ambient temperatures.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 5","pages":"131-141"},"PeriodicalIF":2.4,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/a3/21-00030.PMC8569133.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39612328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of Endurance Exercise on Basement Membrane in the Soleus Muscle of Aged Rats. 耐力运动对老年大鼠比目鱼肌基底膜的影响。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-10-29 Epub Date: 2021-10-27 DOI: 10.1267/ahc.21-00057

Yuji Kanazawa, Mamoru Nagano, Satoshi Koinuma, Shinichi Sugiyo, Yasufumi Shigeyoshi

引用次数: 4

Dexmedetomidine and Phosphocreatine Post-treatment Provides Protection against Focal Cerebral Ischemia-reperfusion Injury in Rats. 右美托咪定和磷酸肌酸对大鼠局灶性脑缺血再灌注损伤的保护作用。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2021-08-25 DOI: 10.1267/ahc.21-00040

Xiaofen Sun, Fang Kang, Yuxian Shen, Yujun Shen, Juan Li

{"title":"Dexmedetomidine and Phosphocreatine Post-treatment Provides Protection against Focal Cerebral Ischemia-reperfusion Injury in Rats.","authors":"Xiaofen Sun, Fang Kang, Yuxian Shen, Yujun Shen, Juan Li","doi":"10.1267/ahc.21-00040","DOIUrl":"https://doi.org/10.1267/ahc.21-00040","url":null,"abstract":"In this study we investigated the neuroprotective efficacy of dexmedetomidine (Dex) and phosphocreatine (PCr) alone or in combination in a rat model of focal cerebral ischemia-reperfusion injury (I/R). I/R was induced by intraluminal middle cerebral artery occlusion (MCAO) and reperfusion. Male Sprague-Dawley rats were randomly allocated to the Sham group and I/R group, and the I/R group was further divided into three subgroups: Dex (9 μg.kg-1 Dex), PCr (180 mg.kg-1 PCr) and Dex + PCr (9 μg.kg-1 Dex + 180 mg.kg-1 PCr). All treatments were given intravenously at the onset of reperfusion. After 24 hr of reperfusion, the neurological deficit score (NDS) was determined and a magnetic resonance imaging (MRI) scan was performed. Serum concentrations of malonaldehyde (MDA) and 4-hydroxynonenal (4-HNE) were measured and cerebral infarct volume was estimated by triphenyl tetrazolium chloride (TTC) staining. Blood brain barrier, neuronal and mitochondrial damage was assessed by optical and electron microscopy. Neuronal injury was further assessed using double cleaved caspase-3 and NeuN immunofluorescent staining. Compared with group I/R, Dex and PCr significantly reduced the neurological deficit score (P < 0.01), infarct volume (P < 0.01), and brain blood barrier, neuronal and mitochondrial damage. The level of oxidative stress (P < 0.001) and neuronal injury (P < 0.001) also decreased and surviving neurons increased (P < 0.001). Compared with Dex or PCr alone, the combination treatment had overall greater effects (P < 0.05). These results indicate that posttreatment with Dex or PCr decreases focal cerebral I/R injury and that these agents in combination have greater protective effects than each alone.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"54 4","pages":"105-113"},"PeriodicalIF":2.4,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9720044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3