Cell Chemical Biology最新文献

Insights into lysophosphatidylserine recognition and Gα12/13-coupling specificity of P2Y10 透视 P2Y10 的溶血磷脂酰丝氨酸识别和 Gα12/13 偶联特异性

IF 8.6 1区生物学

Cell Chemical Biology Pub Date : 2024-09-11 DOI: 10.1016/j.chembiol.2024.08.005

Han Yin, Nozomi Kamakura, Yu Qian, Manae Tatsumi, Tatsuya Ikuta, Jiale Liang, Zhenmei Xu, Ruixue Xia, Anqi Zhang, Changyou Guo, Asuka Inoue, Yuanzheng He

{"title":"Insights into lysophosphatidylserine recognition and Gα12/13-coupling specificity of P2Y10","authors":"Han Yin, Nozomi Kamakura, Yu Qian, Manae Tatsumi, Tatsuya Ikuta, Jiale Liang, Zhenmei Xu, Ruixue Xia, Anqi Zhang, Changyou Guo, Asuka Inoue, Yuanzheng He","doi":"10.1016/j.chembiol.2024.08.005","DOIUrl":"https://doi.org/10.1016/j.chembiol.2024.08.005","url":null,"abstract":"The lysophosphatidylserine (LysoPS) receptor P2Y10, also known as LPS2, plays crucial roles in the regulation of immune responses and holds promise for the treatment of autoimmune diseases. Here, we report the cryoelectron microscopy (cryo-EM) structure of LysoPS-bound P2Y10 in complex with an engineered G13 heterotrimeric protein. The structure and a mutagenesis study highlight the predominant role of a comprehensive polar network in facilitating the binding and activation of the receptor by LysoPS. This interaction pattern is preserved in GPR174, but not in GPR34. Moreover, our structural study unveils the essential interactions that underlie the Gα13 engagement of P2Y10 and identifies key determinants for Gα12-vs.-Gα13-coupling selectivity, whose mutations selectively disrupt Gα12 engagement while preserving the intact coupling of Gα13. The combined structural and functional studies provide insights into the molecular mechanisms of LysoPS recognition and Gα12/13 coupling specificity.","PeriodicalId":265,"journal":{"name":"Cell Chemical Biology","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142166053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical induction of the interaction between AIMP2-DX2 and Siah1 to enhance ubiquitination 化学诱导 AIMP2-DX2 和 Siah1 之间的相互作用以增强泛素化

IF 8.6 1区生物学

Cell Chemical Biology Pub Date : 2024-09-10 DOI: 10.1016/j.chembiol.2024.08.004

Dae Gyu Kim, Minkyoung Kim, Ja-il Goo, Jiwon Kong, Dipesh S. Harmalkar, Qili Lu, Aneesh Sivaraman, Hossam Nada, Sreenivasulu Godesi, Hwayoung Lee, Mo Eun Song, Eunjoo Song, Kang-Hyun Han, Woojin Kim, Pilhan Kim, Won Jun Choi, Chang Hoon Lee, Sunkyung Lee, Yongseok Choi, Sunghoon Kim, Kyeong Lee

引用次数: 0

The best of both worlds: Chemigenetic fluorescent sensors for biological imaging 两全其美：用于生物成像的化学基因荧光传感器

IF 8.6 1区生物学

Cell Chemical Biology Pub Date : 2024-09-04 DOI: 10.1016/j.chembiol.2024.08.002

Kelvin K. Tsao, Shosei Imai, Michael Chang, Saaya Hario, Takuya Terai, Robert E. Campbell

引用次数: 0

A chemical screen identifies PRMT5 as a therapeutic vulnerability for paclitaxel-resistant triple-negative breast cancer 化学筛选发现 PRMT5 是紫杉醇耐药三阴性乳腺癌的治疗漏洞

IF 8.6 1区生物学

Cell Chemical Biology Pub Date : 2024-09-03 DOI: 10.1016/j.chembiol.2024.08.003

KeJing Zhang, Juan Wei, SheYu Zhang, Liyan Fei, Lu Guo, Xueying Liu, YiShuai Ji, WenJun Chen, Felipe E. Ciamponi, WeiChang Chen, MengXi Li, Jie Zhai, Ting Fu, Katlin B. Massirer, Yang Yu, Mathieu Lupien, Yong Wei, Cheryl. H. Arrowsmith, Qin Wu, WeiHong Tan

{"title":"A chemical screen identifies PRMT5 as a therapeutic vulnerability for paclitaxel-resistant triple-negative breast cancer","authors":"KeJing Zhang, Juan Wei, SheYu Zhang, Liyan Fei, Lu Guo, Xueying Liu, YiShuai Ji, WenJun Chen, Felipe E. Ciamponi, WeiChang Chen, MengXi Li, Jie Zhai, Ting Fu, Katlin B. Massirer, Yang Yu, Mathieu Lupien, Yong Wei, Cheryl. H. Arrowsmith, Qin Wu, WeiHong Tan","doi":"10.1016/j.chembiol.2024.08.003","DOIUrl":"https://doi.org/10.1016/j.chembiol.2024.08.003","url":null,"abstract":"Paclitaxel-resistant triple negative breast cancer (TNBC) remains one of the most challenging breast cancers to treat. Here, using an epigenetic chemical probe screen, we uncover an acquired vulnerability of paclitaxel-resistant TNBC cells to protein arginine methyltransferases (PRMTs) inhibition. Analysis of cell lines and in-house clinical samples demonstrates that resistant cells evade paclitaxel killing through stabilizing mitotic chromatin assembly. Genetic or pharmacologic inhibition of PRMT5 alters RNA splicing, particularly intron retention of aurora kinases B (AURKB), leading to a decrease in protein expression, and finally results in selective mitosis catastrophe in paclitaxel-resistant cells. In addition, type I PRMT inhibition synergies with PRMT5 inhibition in suppressing tumor growth of drug-resistant cells through augmenting perturbation of AURKB-mediated mitotic signaling pathway. These findings are fully recapitulated in a patient-derived xenograft (PDX) model generated from a paclitaxel-resistant TNBC patient, providing the rationale for targeting PRMTs in paclitaxel-resistant TNBC.","PeriodicalId":265,"journal":{"name":"Cell Chemical Biology","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant synthetic genomics: Big lessons from the little yeast 植物合成基因组学：从小酵母中汲取大教训

IF 8.6 1区生物学

Cell Chemical Biology Pub Date : 2024-08-29 DOI: 10.1016/j.chembiol.2024.08.001

Hao Ye, Guangyu Luo, Zhenwu Zheng, Xiaofang Li, Jie Cao, Jia Liu, Junbiao Dai

引用次数: 0

Engineering acyclovir-induced RNA nanodevices for reversible and tunable control of aptamer function. 设计阿昔洛韦诱导的 RNA 纳米器件，实现对适配体功能的可逆和可调控制。

IF 6.6 1区生物学

Cell Chemical Biology Pub Date : 2024-08-26 DOI: 10.1016/j.chembiol.2024.07.017

Timo Hagen, Jacob L Litke, Nahian Nasir, Qian Hou, Samie R Jaffrey

{"title":"Engineering acyclovir-induced RNA nanodevices for reversible and tunable control of aptamer function.","authors":"Timo Hagen, Jacob L Litke, Nahian Nasir, Qian Hou, Samie R Jaffrey","doi":"10.1016/j.chembiol.2024.07.017","DOIUrl":"10.1016/j.chembiol.2024.07.017","url":null,"abstract":"Small molecule-regulated RNA devices have the potential to modulate diverse aspects of cellular function, but the small molecules used to date have potential toxicities limiting their use in cells. Here we describe a method for creating drug-regulated RNA nanodevices (RNs) using acyclovir, a biologically compatible small molecule with minimal toxicity. Our modular approach involves a scaffold comprising a central F30 three-way junction, an integrated acyclovir aptamer on the input arm, and a variable effector-binding aptamer on the output arm. This design allows for the rapid engineering of acyclovir-regulated RNs, facilitating temporal, tunable, and reversible control of intracellular aptamers. We demonstrate the control of the Broccoli aptamer and the iron-responsive element (IRE) by acyclovir. Regulating the IRE with acyclovir enables precise control over iron-regulatory protein (IRP) sequestration, consequently promoting the inhibition of ferroptosis. Overall, the method described here provides a platform for transforming aptamers into acyclovir-controllable antagonists against physiologic target proteins.","PeriodicalId":265,"journal":{"name":"Cell Chemical Biology","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemoproteomics reveals immunogenic and tumor-associated cell surface substrates of ectokinase CK2α 化学蛋白质组学揭示了外激酶 CK2α 的免疫原性和肿瘤相关细胞表面底物

IF 8.6 1区生物学

Cell Chemical Biology Pub Date : 2024-08-22 DOI: 10.1016/j.chembiol.2024.07.018

Corleone S. Delaveris, Sophie Kong, Jeff Glasgow, Rita P. Loudermilk, Lisa L. Kirkemo, Fangzhu Zhao, Fernando Salangsang, Paul Phojanakong, Juan Antonio Camara Serrano, Veronica Steri, James A. Wells

{"title":"Chemoproteomics reveals immunogenic and tumor-associated cell surface substrates of ectokinase CK2α","authors":"Corleone S. Delaveris, Sophie Kong, Jeff Glasgow, Rita P. Loudermilk, Lisa L. Kirkemo, Fangzhu Zhao, Fernando Salangsang, Paul Phojanakong, Juan Antonio Camara Serrano, Veronica Steri, James A. Wells","doi":"10.1016/j.chembiol.2024.07.018","DOIUrl":"https://doi.org/10.1016/j.chembiol.2024.07.018","url":null,"abstract":"Foreign epitopes for immune recognition provide the basis of anticancer immunity. Due to the high concentration of extracellular adenosine triphosphate in the tumor microenvironment, we hypothesized that extracellular kinases (ectokinases) could have dysregulated activity and introduce aberrant phosphorylation sites on cell surface proteins. We engineered a cell-tethered version of the extracellular kinase CK2α, demonstrated it was active on cells under tumor-relevant conditions, and profiled its substrate scope using a chemoproteomic workflow. We then demonstrated that mice developed polyreactive antisera in response to syngeneic tumor cells that had been subjected to surface hyperphosphorylation with CK2α. Interestingly, these mice developed B cell and CD4+ T cell responses in response to these antigens but failed to develop a CD8+ T cell response. This work provides a workflow for probing the extracellular phosphoproteome and demonstrates that extracellular phosphoproteins are immunogenic even in a syngeneic system.","PeriodicalId":265,"journal":{"name":"Cell Chemical Biology","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142023133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging biochemical, microbial and immunological evidence in the search for why HLA-B^∗27 confers risk for spondyloarthritis. 寻找 HLA-B∗27 为何会导致脊柱关节炎风险的生化、微生物和免疫学证据。

IF 6.6 1区生物学

Cell Chemical Biology Pub Date : 2024-08-20 DOI: 10.1016/j.chembiol.2024.07.012

Eric M Brown, Phuong N U Nguyen, Ramnik J Xavier

{"title":"Emerging biochemical, microbial and immunological evidence in the search for why HLA-B∗27 confers risk for spondyloarthritis.","authors":"Eric M Brown, Phuong N U Nguyen, Ramnik J Xavier","doi":"10.1016/j.chembiol.2024.07.012","DOIUrl":"10.1016/j.chembiol.2024.07.012","url":null,"abstract":"The strong association of the human leukocyte antigen B∗27 alleles (HLA-B∗27) with spondyloarthritis and related rheumatic conditions has long fascinated researchers, yet the precise mechanisms underlying its pathogenicity remain elusive. Here, we review how interplay between the microbiome, the immune system, and the enigmatic HLA-B∗27 could trigger spondyloarthritis, with a focus on whether HLA-B∗27 presents an arthritogenic peptide. We propose mechanisms by which the unique biochemical characteristics of the HLA-B∗27 protein structure, particularly its peptide binding groove, could dictate its propensity to induce pathological T cell responses. We further provide new insights into how TRBV9+ CD8+ T cells are implicated in the disease process, as well as how the immunometabolism of T cells modulates tissue-specific inflammatory responses in spondyloarthritis. Finally, we present testable models and suggest approaches to this problem in future studies given recent advances in computational biology, chemical biology, structural biology, and small-molecule therapeutics.","PeriodicalId":265,"journal":{"name":"Cell Chemical Biology","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142015826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors 粘附 G 蛋白偶联受体中系留激动剂信号的异质性

IF 6.6 1区生物学

Cell Chemical Biology Pub Date : 2024-08-15 DOI: 10.1016/j.chembiol.2024.03.004

引用次数: 0

Feel the breeze: Opening the therapeutic window with RIPTACs and induced proximity 感受微风利用 RIPTAC 和诱导接近打开治疗之窗

IF 6.6 1区生物学

Cell Chemical Biology Pub Date : 2024-08-15 DOI: 10.1016/j.chembiol.2024.07.013

引用次数: 0