Biochimie最新文献

{"title":"Antibacterial activity and mechanistic insights of Lucilia illustris antimicrobial peptide Cecropin A2 against Pseudomonas aeruginosa","authors":"Liang Chen,&nbsp;Lei Xu,&nbsp;Mengyue Wang,&nbsp;Qilong Zhao,&nbsp;Chunlan Wei,&nbsp;Qiang Wang,&nbsp;Shangshang Ma,&nbsp;Weiping Cao","doi":"10.1016/j.biochi.2026.09.005","DOIUrl":"10.1016/j.biochi.2026.09.005","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) poses a serious public health threat due to multidrug resistance and biofilm formation. This study investigated the antibacterial mechanisms of the antimicrobial peptide, Cecropin A2 (CA2), against <em>P. aeruginosa.</em> The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CA2 against <em>P. aeruginosa</em> ATCC 9027 (PA ATCC 9027) were determined by broth microdilution. Antibacterial activity was evaluated using growth curves and time-kill assays. The mechanism was explored by assessing membrane integrity (outer/inner membrane permeability, SEM, and fluorescence microscopy), and intracellular responses (ATP, SDH activity, and ROS). Biofilm effects were assessed by crystal violet staining (biomass) and viable cell counting (biofilm-embedded bacteria). The MIC and MBC of CA2 against PA ATCC 9027 were 37.34 μM and 74.68 μM, respectively. CA2 exhibited moderate antibacterial activity against PA ATCC 9027. Scanning electron microscopy (SEM) revealed marked morphological damage after treatment. CA2 affected intracellular metabolism, potentially interacted with genomic DNA, and reduced biofilm biomass. Cecropin A2 exhibits concentration-dependent in vitro antibacterial activity against <em>P. aeruginosa</em> ATCC 9027, providing mechanistic insights and a theoretical basis for the development of alternative antimicrobial strategies.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"250 ","pages":"Pages 195-204"},"PeriodicalIF":3.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148979050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the role of fungal peptide cytotoxin candidalysin in candidiasis and non-candidiasis diseases","authors":"Hanyu Zhu,&nbsp;Xue Wang,&nbsp;Jing Shao","doi":"10.1016/j.biochi.2026.09.004","DOIUrl":"10.1016/j.biochi.2026.09.004","url":null,"abstract":"<div><div><em>Candida albicans</em> is a commonly encountered opportunistic fungus that can cause both superficial discomforts and life-threatening invasive fungal infections. Ever-increasing reports have shown that <em>C. albicans</em> is also readily implicated in a set of metabolic and autoimmune diseases. Yeast-to-hypha transition is a hallmark of increased virulence and invasion of <em>C. albicans</em>, and candidalysin is the first peptide toxin secreted by <em>C. albicans</em> hyphae. Candidalysin contributes to fungal penetration across epithelial barrier via cytolytic activity, as well as the maintenance of commensalism and pathogenicity of <em>Candida albicans</em>. To specifically depict the role of candidalysin in multiple candidiasis and <em>C. albicans</em> associated infections, in this review, we focus on the recent advancements of candidalysin in oropharyngeal candidiasis (OPC), vulvovaginal candidiasis (VVC), disseminated candidaemia and sepsis. Meanwhile, we also pay close attention to the potential pathogenic mechanism of candidalysin in several <em>C. albicans</em> associated diseases including inflammatory bowel disease (IBD), asthma, Alzheimer's disease (AD) and alcohol-associated liver diseases (ALD). Based on these achievements, we propose a list of unresolved questions of interest for future investigation on biological functions and therapeutic potential of candidalysin in fungal infections caused by <em>C. albicans</em>. Unlocking the mode of action of candidalysin is beneficial for deep understanding of how <em>C. albicans</em> breaks through mucosal or blood brain barrier to colonize the niche via candidalysin and for rational design of fungal vaccine against candidalysin for clinical applications. Q1: Revision based on the comment 1 by Reviewer 1.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"250 ","pages":"Pages 185-194"},"PeriodicalIF":3.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148984464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inside front cover-EDB","authors":"","doi":"10.1016/S0300-9084(26)00259-2","DOIUrl":"10.1016/S0300-9084(26)00259-2","url":null,"abstract":"","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"250 ","pages":"Page IFC"},"PeriodicalIF":3.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149350331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of bacterial cellulose: Biosynthesis strategies, functionalization and biomedical marketing","authors":"Ahmed K. Saleh ,&nbsp;Tarek H. Taha ,&nbsp;Hussain Alenezi ,&nbsp;Esmail M. El-Fakharany ,&nbsp;Abdulrahman Mohammed Alhudhaibi ,&nbsp;Shaikha A. Albatli ,&nbsp;Marwa Yousry A. Mohamed ,&nbsp;Hamada El-Gendia","doi":"10.1016/j.biochi.2026.02.003","DOIUrl":"10.1016/j.biochi.2026.02.003","url":null,"abstract":"<div><div>Bacterial cellulose (BC) is reported as an extracellular polysaccharide distinguished by its exceptional purity, mechanical features, and biocompatibility, making it an attractive material for applications in food, plant tissue culture, and Biomedicine. Traditionally, BC production relies on chemically defined synthetic media under either static or agitated fermentation conditions. However, the high production cost remains a critical barrier, limiting its large-scale utilization and broader adoption. To address this challenge, recent advances in sustainable strategies, such as employing agro-industrial byproducts and low-cost carbon sources, have significantly reduced costs while improving yield and functional properties. In particular, diverse environmental waste streams, including agricultural residues, industrial wastes, and food processing byproducts, have been explored as renewable substrates for BC synthesis. BC can be obtained through static fermentation, yielding gelatinous films (pellicles), or agitated fermentation, generating suspended fibers or pellets, each with unique structural features. Moreover, innovative functionalization approaches, including <em>in situ</em> and <em>ex situ</em> modifications, incorporation of bioactive agents, and the development of BC-based nanocomposites, have further expanded its biomedical potential. This review emphasizes sustainable strategies to overcome the cost limitations of BC production, while also highlighting recent advances in functionalization techniques and their pivotal role in advancing medical applications, including cartilage engineering, bone regeneration, wound healing, and dentistry.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 7-28"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146121249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights of immune evasion by Orf Virus: the interaction and functional impact of viral OH1 phosphatase on STAT1","authors":"Dario Porley ,&nbsp;Natalia Olivero-Deibe ,&nbsp;Vanina Astrada ,&nbsp;Valentin Bransolle ,&nbsp;Danilo Segovia ,&nbsp;Mariana Margenat ,&nbsp;María Magdalena Portela ,&nbsp;Andrea Villarino ,&nbsp;Gwenaëlle André ,&nbsp;Mabel Berois","doi":"10.1016/j.biochi.2026.02.006","DOIUrl":"10.1016/j.biochi.2026.02.006","url":null,"abstract":"<div><div>Orf virus, a member of the <em>Parapoxvirus</em> genus within the <em>Poxvirus</em> family, is the causative agent of Contagious Ecthyma, a zoonotic infection primarily affecting goats and sheep. The immune response against Orf virus is short-lived, enabling the virus to repeatedly infect animals, regardless of their vaccination status. Several virulence factors, including the OH1 tyrosine phosphatase, are responsible for the modulation of the host immune response. Here, we report the direct interaction between the viral OH1 and the human transcription factor STAT1 identified as a physiological OH1 substrate. Indeed, our results demonstrate that OH1 dephosphorylates STAT1 at pTyr701, resulting in a subsequent impairing of its nuclear import. By employing protein-protein docking and molecular dynamics simulations, we modelled the STAT1-OH1 complex, phosphorylated or not, and dissected the structural basis of its mutual recognition. Also, we identified additional potential substrates of OH1 that show to be involved in cell trafficking, thereby expanding our understanding of the host-triggered immune responses elicited by Poxviruses. Overall, this study provides insights into the molecular mechanisms underlying immune evasion in Orf virus, extending our knowledge towards new therapeutic strategies against this pathogen.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 92-101"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146127716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulation characterization of novel transcription regulator JTY_2262 in Mycobacterium bovis BCG","authors":"Jinmiao Song ,&nbsp;Hui Wang ,&nbsp;Mingrui Sun ,&nbsp;Mei Li ,&nbsp;Yang Zhang ,&nbsp;Jiayin Xing ,&nbsp;Shuxian Wang ,&nbsp;Ren Fang ,&nbsp;Xiaotian Li ,&nbsp;Siguo Liu ,&nbsp;Zhaoli Li ,&nbsp;Ningning Song","doi":"10.1016/j.biochi.2026.01.013","DOIUrl":"10.1016/j.biochi.2026.01.013","url":null,"abstract":"<div><div><em>Mycobacterium tuberculosis</em> (Mtb) can survive for a long time <em>in vivo</em> and evade host immune attacks, primarily through transcriptional regulation mediated by transcription regulators. JTY_2262 (homologous with Rv2250c) belongs to the Tetracycline Repressor (TetR) family of regulators, and its function is currently unclear. In this study, we combined JTY_2262 overexpressed transcriptomics and electrophoretic mobility shift assays (EMSA) to identify novel targets regulated by JTY_2262. The cofactors containing Cys, V_C, V_B1, V_B3, V_B6, Pb²⁺, Cu²⁺ and Li ⁺ inhibit the binding between JTY_2262 and the <em>JTY_2045</em> (homologous with <em>rv2031c</em>) promoter. Overall, this study demonstrates that JTY_2262 is a versatile regulator. Under different environmental conditions, JTY_2262 senses concentration variations of specific cofactors. By modulating its DNA-binding affinity accordingly, JTY_2262 regulates targeted gene expression, enabling bacteria to adapt their metabolic machinery and physiological state to better adapt the changing environment.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 29-39"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the sweetness of natural plant-derived sweeteners mogroside IV and V and their interactions with human sweet taste receptor","authors":"Binbin Yao ,&nbsp;Cunli Dou ,&nbsp;Yuyu Zhang ,&nbsp;Bo Liu","doi":"10.1016/j.biochi.2026.02.008","DOIUrl":"10.1016/j.biochi.2026.02.008","url":null,"abstract":"<div><div>Mogrosides are an important type of natural plant-derived sweeteners, which are used in foods and medicines. However, their sweetness and structure-activity relationship, especially those for the representative members mogrosides IV and V, have been rarely reported. In the present study, we characterized the sweet taste properties (threshold values, etc) of mogrosides IV and V with a cell-based calcium mobilization assay, showing that mogrosides represent a category of primitive sweet compounds which are edible by primates as well as other mammals. Furthermore, it was demonstrated that the sweetness of mogrosides can be inhibited by a classical sweet taste modulator, lactisole. Moreover, the binding site of mogroside IV in human Tas1R2 Venus flytrap domain was identified with the method of molecular docking, which was further validated by functional mutagenesis analysis. These results provide helpful guidelines for further exploring the structure-activity relationship and molecular design of mogrosides.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 64-69"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146138261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-mediated regulation of hypoxic kidney adaptation in naked mole-rats (Heterocephalus glaber)","authors":"Yasser Attaie ,&nbsp;Sarah A. Breedon ,&nbsp;Saif Rehman ,&nbsp;Mohammad Ojaghi ,&nbsp;Karen L. Kadamani ,&nbsp;Matthew E. Pamenter ,&nbsp;Kenneth B. Storey","doi":"10.1016/j.biochi.2026.02.007","DOIUrl":"10.1016/j.biochi.2026.02.007","url":null,"abstract":"<div><div>MicroRNAs play crucial roles in post-transcriptional regulation during environmental stress, yet their contribution to hypoxia adaptation in naturally hypoxia-tolerant species remains poorly understood. Here, we characterized the miRNA expression profile in kidneys of the naked mole-rat (<em>Heterocephalus glaber</em>), a subterranean rodent renowned for its exceptional hypoxia tolerance.</div><div>Small RNA from naked-mole rat kidneys was sequenced under normoxic and hypoxic conditions to predict miRNA-mRNA interactions during low-oxygen stress. Bioinformatic analysis identified differentially expressed miRNAs and used pathway enrichment to predict regulatory mechanisms controlling kidney adaptation to hypoxia. Upregulated miRNAs, including let-7c-5p and miR-29a-3p target genes involved in cell cycle progression, extracellular matrix remodeling, and metabolic pathways, corresponding with negative enrichment of these processes. Conversely, downregulated miRNAs relieve inhibition of transcripts involved in chromatin remodeling, RNA processing, and immune signaling, aligning with positive enrichment of these adaptive pathways. Gene Ontology cellular component analysis suggested systematic subcellular reorganization, with suppression of extracellular and secretory compartments and enhancement of nuclear, RNA processing, and cytoskeletal structures. Notably, hypoxia induced upregulation of ribonucleoprotein complexes, spliceosomal machinery, and histone methyltransferase complexes, while downregulating extracellular matrix components and secretory pathway structures. This coordinated miRNA response appears to optimize energy utilization by suppressing non-essential pathways while selectively enhancing survival mechanisms through targeted post-transcriptional control; however further studies are required to confirm these findings. Our findings provide novel insights into the molecular mechanisms underlying the remarkable hypoxia tolerance of naked mole-rats and highlight miRNA-mediated regulation as a key adaptive strategy in mammalian hypoxic survival.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 54-63"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global lysine 2-hydroxyisobutyrylation profiling reveals the growth mechanism of Lactobacillus paracasei PC-01 in high-density cultures","authors":"Shijia Shen ,&nbsp;Runzhi Zhou ,&nbsp;Hua Wang ,&nbsp;Hongyang Li ,&nbsp;Shuaisen Gao ,&nbsp;Jie Yu","doi":"10.1016/j.biochi.2026.02.002","DOIUrl":"10.1016/j.biochi.2026.02.002","url":null,"abstract":"<div><div>The importance of lysine 2-hydroxyisobutyrylation (Khib) in regulating biological processes has become increasingly apparent, but its functional significance in prokaryotes, especially in the <em>Lactobacillus paracasei</em> PC-01, is poorly understood. In this study, the regulatory mechanism of Khib in high-density fermentation of <em>L. paracasei</em> PC-01 was investigated using mass spectrometry proteomics. In total, 6783 Khib sites on 1361 proteins were identified, with 73.48% proteins containing multiple sites. GO and KEGG enrichment analysis showed Khib affected cellular functions and metabolic pathways, especially those involved in ribosomal activity and protein biosynthesis. Subsequently, a Khib-mediated regulatory network of <em>L. paracasei</em> PC-01 revealed most of the significantly upregulated enzymes in the glycolysis, TCA cycle, purine metabolism, amino acid metabolism, and fatty acid biosynthesis pathways were modified by Khib. This study elucidated the biological function of Khib modification in <em>L. paracasei</em> during high-density cultures and provides theoretical basis for the preparation of highly active probiotic preparations.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 70-79"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146121261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled CRISPR/Cas12a activation via DNAzyme-mediated splitting of chimeric substrate for lead detection","authors":"Peiying Zhang ,&nbsp;Meng Shen ,&nbsp;Lihua Ding,&nbsp;Leiliang He,&nbsp;Yongjun Wu,&nbsp;Songcheng Yu","doi":"10.1016/j.biochi.2026.02.001","DOIUrl":"10.1016/j.biochi.2026.02.001","url":null,"abstract":"<div><div>Chronic lead exposure poses severe threats to human health, which demands a rapid detection strategy beyond conventional instrumentation-dependent approaches. While CRISPR/Cas12a systems offer promising alternatives through <em>trans</em>-cleavage activity, conventional Pb²⁺ biosensors relying on DNAzyme-generated intact activators suffer from high background signals due to interference from uncleaved substrates. To address this limitation, we developed a steric-hindrance-controlled activation strategy by employing a chimeric DNAzyme substrate (Sub) that prevents Cas12a binding until Pb²⁺-dependent cleavage occurs. This DNAzyme-mediated splitting releases two fragments (A1/A2) that rearrange into split activators, triggering the CRISPR/Cas12a <em>trans</em>-cleavage of a quenched reporter (6-FAM/BHQ1). Under the optimal condition, the sensor achieved a linear detection range of 2.5–25 μM (R² = 0.998) with 2.18 μM LOD and high selectivity against interferents. Validation in tap water matrices demonstrated 98.6%–102.6% recovery (RSD 3.0%–7.5%), which showed robustness in real samples. This split-activator design paradigm eliminates background from uncleaved substrates without additional pretreatment steps to provide a versatile template for converting metal ions into CRISPR-detectable signals.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"244 ","pages":"Pages 1-6"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146121310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}