Biochimie最新文献_第2页

Lunatin-1: a membrane-disruptive peptide isolated from Hadruroides lunatus scorpion venom with cytotoxicity against MDA-MB-231 breast cancer cell line 月蝎蛋白-1：一种从月蝎毒液中分离的膜破坏肽，对乳腺癌细胞系MDA-MB-231具有细胞毒性。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-21 DOI: 10.1016/j.biochi.2025.06.006

Kamila de Sousa Gomes , Brenda Raíssa-Oliveira , Stefan Schildknecht , Maria Elena de Lima , Marcella Nunes Melo-Braga , Dawidson Assis Gomes , Adriano Monteiro de Castro Pimenta , Thiago Verano-Braga , Marcel Leist , Elaine Maria de Souza-Fagundes

{"title":"Lunatin-1: a membrane-disruptive peptide isolated from Hadruroides lunatus scorpion venom with cytotoxicity against MDA-MB-231 breast cancer cell line","authors":"Kamila de Sousa Gomes , Brenda Raíssa-Oliveira , Stefan Schildknecht , Maria Elena de Lima , Marcella Nunes Melo-Braga , Dawidson Assis Gomes , Adriano Monteiro de Castro Pimenta , Thiago Verano-Braga , Marcel Leist , Elaine Maria de Souza-Fagundes","doi":"10.1016/j.biochi.2025.06.006","DOIUrl":"10.1016/j.biochi.2025.06.006","url":null,"abstract":"<div><div>Membrane-disrupting peptides - including antimicrobial and antitumor peptides - disrupt cell membrane through pore formation. Strategies for using these peptides as adjuvants in cancer treatment regimens have been investigated. In the context of a high recurrence rate and ineffective postoperative adjuvant chemotherapy treatment in triple-negative breast cancer (TNBC), the potential antitumor cytotoxic effect of Lunatin-1 was evaluated for the first time in the TNBC cell line MDA-MB-231. Synthetic Lunatin-1 was purified and its purity confirmed by mass spectrometry. Cytotoxicity of this peptide against MDA-MB-231 cells was associated with a rapid increase in propidium iodide-positive cells and LDH release through the loss of membrane integrity in a concentration and time-dependent manner. Staining with CellMask Deep Red to outline the cell membrane showed its disruption 5 min after Lunatin-1 treatment, which was not associated with necroptosis. Ultrastructural analysis by scanning electron microscopy showed membrane damage due to microvilli reduction and increased density of pores per cell compared to untreated cells. We concluded that Lunatin-1 is a membrane-disruptive peptide in this cellular model, highlighting it as an interesting tool for conjugating with cancer markers or anticancer drugs.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"236 ","pages":"Pages 74-86"},"PeriodicalIF":3.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144478233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testis-specific RNA methyltransferase NSUN7 contains a re-arranged catalytic site 睾丸特异性RNA甲基转移酶NSUN7包含一个重排的催化位点。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-20 DOI: 10.1016/j.biochi.2025.06.011

Ekaterina A. Guseva , Olga A. Averina , Vitaly S. Buev , Elizaveta E. Bragina , Oleg A. Permyakov , Anastasia V. Priymak , Mariia A. Emelianova , Evgeny A. Romanov , Olga O. Grigoryeva , Vasily N. Manskikh , Philipp I. Pletnev , Petr V. Sergiev

{"title":"Testis-specific RNA methyltransferase NSUN7 contains a re-arranged catalytic site","authors":"Ekaterina A. Guseva , Olga A. Averina , Vitaly S. Buev , Elizaveta E. Bragina , Oleg A. Permyakov , Anastasia V. Priymak , Mariia A. Emelianova , Evgeny A. Romanov , Olga O. Grigoryeva , Vasily N. Manskikh , Philipp I. Pletnev , Petr V. Sergiev","doi":"10.1016/j.biochi.2025.06.011","DOIUrl":"10.1016/j.biochi.2025.06.011","url":null,"abstract":"<div><div>The RNA methyltransferase NSUN7 has been reported to be involved in the regulation of longitudinal columns positioning in sperm flagella, but its catalytic mechanism remains unclear. In this study, we investigated the functional role of NSUN7's methylation in the longitudinal column positioning by generating a mouse strain with a substitution of the putative catalytic cysteine in motif IV to alanine (<em>Nsun7</em><sup><em>C382A</em></sup>). Contrary to predictions based on the typical reaction mechanism of NOP2/Sun family methyltransferases, <em>Nsun7</em><sup><em>C382A</em></sup> mice did not exhibit any phenotypical characteristics of knockouts and had normal fertility, sperm motility, and longitudinal column positioning, similar to wild-type mice. Structural modelling suggests that NSUN7 possesses an unusual catalytic site composition, including three highly conserved cysteines from motifs IV, VI and VIII. The distance between the canonical catalytic cysteines in motifs IV and VI is significantly greater than in related methyltransferases, while a cysteine from motif VIII is positioned closer to motif VI. These findings allow us to assume that NSUN7 may have an essential function in the spermatogenesis of mice independent on its methyltransferase activity.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"236 ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening inhibitory peptides against sphingomyelinase D from the livestock tick Rhipicephalus microplus using phage display technology 利用噬菌体展示技术筛选家畜小头蜱鞘磷脂酶D抑制肽。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-16 DOI: 10.1016/j.biochi.2025.06.009

Fernando A.A. Silva , Gabriel C.A. Costa , Ricardo J.S. Torquato , Maria A. Juliano , Aparecida S. Tanaka

{"title":"Screening inhibitory peptides against sphingomyelinase D from the livestock tick Rhipicephalus microplus using phage display technology","authors":"Fernando A.A. Silva , Gabriel C.A. Costa , Ricardo J.S. Torquato , Maria A. Juliano , Aparecida S. Tanaka","doi":"10.1016/j.biochi.2025.06.009","DOIUrl":"10.1016/j.biochi.2025.06.009","url":null,"abstract":"<div><div>The cattle tick <em>Rhipicephalus microplus</em>, which is distributed worldwide, causes significant economic loss to the livestock industry. A sphingomyelinase (SMase) D-like protein was previously identified in <em>R. microplus</em> saliva (<em>Rm</em>SMase) and characterized in terms of its cofactors, optimum pH, and products generated from sphingomyelin hydrolysis. SMases are present in the venom of spiders from <em>Loxosceles</em> spp. and in the digestive system of non-venomous spiders from <em>Uloborus</em> spp. and are secreted by pathogenic bacteria. In this study A peptide library was screened using Phage Display technology and two out of the three peptides synthesized showed inhibitory activity against RmSMase with sphingomyelin as the substrate. Two of the three synthesized peptides showed inhibitory activity against <em>Rm</em>SMase. Docking analysis suggested that the peptides block access of the substrate to the catalytic site. The RWLWWLW peptide showed competitive behavior (Ki = 5.35 ± 0.46 μM), whereas WLSWLW showed competitive inhibition behavior (Ki = 29.54 ± 6.11 μM). Both RWLWWLW and WLSWLW also inhibited the activity of SMase C from the bacterial pathogen <em>Bacillus cereus</em>, presenting IC<sub>50</sub> values of 4.99 ± 0.07 and 16.94 ± 8.71 μM, respectively. Because SMases have been suggested to be involved in the development of Alzheimer's disease, major depression, and cerebral ischemia, the discovery and characterization of new SMase inhibitors may contribute to the development of alternative treatments. This is the first study to identify peptide inhibitors against tick SMase using phage display technology.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"236 ","pages":"Pages 45-53"},"PeriodicalIF":3.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Park7 (DJ-1), a Parkinson-associated glyoxalase/deglycase: characterization of enzymatic activity in biological samples using fluorescence-based liquid chromatography Park7 (DJ-1)，一种帕金森相关的glyoxalase/deglycase：利用荧光液相色谱法表征生物样品中的酶活性。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-14 DOI: 10.1016/j.biochi.2025.06.005

Nicolas Mathas , Emmanuelle Braud , Erwan Galardon , Lucie Larigot , Mélanie Etheve-Quelquejeu , Marie-Agnès Sari , Beatrice Le Grand , Etienne B. Blanc , Xavier Coumoul , Julien Dairou

{"title":"Park7 (DJ-1), a Parkinson-associated glyoxalase/deglycase: characterization of enzymatic activity in biological samples using fluorescence-based liquid chromatography","authors":"Nicolas Mathas , Emmanuelle Braud , Erwan Galardon , Lucie Larigot , Mélanie Etheve-Quelquejeu , Marie-Agnès Sari , Beatrice Le Grand , Etienne B. Blanc , Xavier Coumoul , Julien Dairou","doi":"10.1016/j.biochi.2025.06.005","DOIUrl":"10.1016/j.biochi.2025.06.005","url":null,"abstract":"<div><div>Glycation is an inevitable nonenzymatic covalent reaction between nucleophilic groups in proteins and nucleotides, and endogenous reducing sugars or dicarbonyls (e.g., methylglyoxal) leading to protein and/or nucleotide covalent modifications. This process initiates a series of dehydrations, oxidations and rearrangements that lead to a myriad of products called advanced glycation end products (AGEs). Previously, we showed that Park7 (or DJ-1) is a protein deglycase that repairs methylglyoxal-glycated biomolecules by acting on early glycation intermediates and releasing the repaired biomolecule and lactate. Moreover, the <em>PARK7</em> gene is associated with recessive and sporadic forms of Parkinson's disease (PD). Thus, over the last few years, the function of Park7 has become an important research topic for understanding the etiology of PD. In this context, the development of sensitive enzymatic assays is crucial to quantify the activity of Park7 and develop specific modulators. Here, we describe a new method for measuring Park7 activity based on the separation and quantification of glycated and unglycated fluorescent nucleotides by Liquid Chromatography (LC). Kinetic and mechanistic analyses using human recombinant Park7 confirmed the reliability of this approach. In addition, this assay was further validated using cellular lysates. Our results indicate that this novel Park7 assay is simple, sensitive, and specific.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 113-121"},"PeriodicalIF":3.3,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidomic analysis of MDCK-parental cells and MDCK-MDR1 cells exposed to GF120918 and clarithromycin highlights the role of P-glycoprotein in sphingolipid transport 对暴露于GF120918和克拉霉素的mdck亲本细胞和MDCK-MDR1细胞的脂质组学分析强调了p -糖蛋白在鞘脂转运中的作用。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-14 DOI: 10.1016/j.biochi.2025.06.007

Théodore Decaix , Romain Magny , David Hozain , Elodie Olivier , Sylvie Gillet , Xavier Declèves , Olivier Laprévote

{"title":"Lipidomic analysis of MDCK-parental cells and MDCK-MDR1 cells exposed to GF120918 and clarithromycin highlights the role of P-glycoprotein in sphingolipid transport","authors":"Théodore Decaix , Romain Magny , David Hozain , Elodie Olivier , Sylvie Gillet , Xavier Declèves , Olivier Laprévote","doi":"10.1016/j.biochi.2025.06.007","DOIUrl":"10.1016/j.biochi.2025.06.007","url":null,"abstract":"<div><div>P-glycoprotein (P-gp) is one of the most studied drug transporters, as it enables the cellular efflux of 50 % of marketed drugs and plays a key role in many drug-drug interactions. While a high number of studies has investigated the use of drug probes to phenotype P-gp activity, identifying endogenous markers remain a not fulfilled challenge. A previous untargeted lipidomic study in healthy volunteers highlighted some sphingolipid species as putative endogenous substrates of P-gp. Based on an untargeted lipidomic analysis of Madin-darby canine kidney parental cells (MDCK-parental cells) and MDCK cells transfected with the MDR1 gene (MDCK-MDR1), the present study aimed to investigate the role of P-gp in intracellular sphingolipid transport and, more generally, to demonstrate the interest of metabolomics strategy in the study of <em>in vitro</em> drug transporters. Using an untargeted lipidomic approach based on liquid chromatography high resolution mass spectrometry, allowed us to detect 131 sphingolipid species within MDCK-parental cells and MDCK-MDR1. Among them, modifications of this sphingolipidome were observed because of MDR1 transfection, mainly involving glucosylceramides. Exposure to two P-gp inhibitors, GF120918 and clarithromycin, increased glucosylceramides, particularly in MDCK-parental cells, probably due to expression of endogenous canine P-gp in this cell line. These results support the hypotheses of a role for P-gp as well as glucosylceramide flippase within the Golgi apparatus of cells. It also provides a proof of concept for understanding pharmaco-metabolomic results in the study of drug transporters in humans.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"236 ","pages":"Pages 21-29"},"PeriodicalIF":3.3,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the FhlA transcriptional activator in metabolic changes in Escherichia coli during fermentation of mixed carbon sources at acidic pH FhlA转录激活因子在酸性混合碳源发酵过程中大肠杆菌代谢变化中的作用。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-12 DOI: 10.1016/j.biochi.2025.06.008

Heghine Gevorgyan , Marine Parsadanyan , Anait Vassilian , Anna Poladyan , Karen Trchounian

引用次数: 0

Utilization of glucose and fructose in bovine embryos assessed by metabolic flux analysis 利用代谢通量分析法评价牛胚胎对葡萄糖和果糖的利用。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-12 DOI: 10.1016/j.biochi.2025.06.004

Daniel J. Lugar , Jaewook Chung , Robert I. Clifford , Halli S. Weiner , Carol L. Keefer , Ganesh Sriram

{"title":"Utilization of glucose and fructose in bovine embryos assessed by metabolic flux analysis","authors":"Daniel J. Lugar , Jaewook Chung , Robert I. Clifford , Halli S. Weiner , Carol L. Keefer , Ganesh Sriram","doi":"10.1016/j.biochi.2025.06.004","DOIUrl":"10.1016/j.biochi.2025.06.004","url":null,"abstract":"<div><div><sup>13</sup>C Metabolic flux analysis (<sup>13</sup>C MFA) is a non-invasive methodology for the measurement of fluxes (rates of carbon flow) throughout a metabolic network and can be used to determine nutrient contributions to central metabolic pathways. <sup>13</sup>C MFA was used in a systemic metabolic investigation of the nutritional physiology of preimplantation bovine blastocysts. Bovine blastocysts were cultured either singly or in groups of five in 40 μl of medium containing <sup>13</sup>C labeled glucose and/or fructose, and the metabolites expelled into the spent media were identified through <sup>13</sup>C MFA of heavy isotope labeling. Similarly, the metabolites in spent media following culture of a bovine trophectoderm cell line (CT-1) were monitored. Flux maps generated using <sup>13</sup>C MFA suggest that less than 10 % of metabolites from the glycolytic pathway were diverted to the pentose phosphate pathway or the tricarboxylic acid cycle in either bovine blastocysts or CT-1 cells with the majority of the hexose (70–80 %) resulting in pyruvate and lactate production.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"236 ","pages":"Pages 10-20"},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lambda exonuclease assisted helicase-dependent amplification CRISPR/Cas12a detection of Listeria monocytogenes Lambda外切酶辅助解旋酶依赖扩增CRISPR/Cas12a检测单核增生李斯特菌。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-09 DOI: 10.1016/j.biochi.2025.06.002

Chen Wang , Qingshuang Wang , Yushi Jin , Chenxi Li , Meiyan Xin , Xue Jiang , Jiayu Wan

{"title":"Lambda exonuclease assisted helicase-dependent amplification CRISPR/Cas12a detection of Listeria monocytogenes","authors":"Chen Wang , Qingshuang Wang , Yushi Jin , Chenxi Li , Meiyan Xin , Xue Jiang , Jiayu Wan","doi":"10.1016/j.biochi.2025.06.002","DOIUrl":"10.1016/j.biochi.2025.06.002","url":null,"abstract":"<div><div>We describe the construction of a protospacer adjacent motif-free CRISPR/Cas12a fluorescent biosensor based on lambda exonuclease (λ-exo) and helicase-dependent amplification (HDA) to detect <em>Listeria monocytogenes</em>(<em>L. monocytogenes</em>). The <em>hlyA</em> gene of <em>L. monocytogenes</em> was amplified by HDA. After λ-exo catalyzed cleavage of 5′ phosphorylated single-stranded DNA of amplification product double-stranded DNA, the double-stranded DNA formed single-stranded DNA (ssDNA). The ssDNA as a substrate activated the <em>trans</em>-cleavage capability of CRISPR/Cas12a to cleave the reporter gene to produce fluorescence signals. Under optimized experimental conditions, the lower limit of <em>L. monocytogenes</em> detection by the fluorescent biosensor was 11.5 CFU/mL, with a linear range of detection from 10<sup>1</sup> to 10<sup>7</sup> CFU/mL. The fluorescent biosensor permits simple and sensitive detection of <em>L. monocytogenes</em> and provides a promising analysis platform for clinical diagnosis and biomedical research without protospacer adjacent motif sequence ssDNA.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 106-112"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144277038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of venom variation and phylogenetic relationships of Micrurus dumerilii from three different regions of Colombia 哥伦比亚3个不同地区小圆尾鼠毒液变异及系统发育关系的评估。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-09 DOI: 10.1016/j.biochi.2025.06.003

Paola Rey-Suárez , Jeisson Gómez-Robles , Julián Fernández , Bruno Lomonte , Mahmood Sasa , Mónica Saldarriaga-Cordoba , Jaime Andrés Pereañez , Omayra Aguilera , Vitelbina Núñez-Rangel

{"title":"Assessment of venom variation and phylogenetic relationships of Micrurus dumerilii from three different regions of Colombia","authors":"Paola Rey-Suárez , Jeisson Gómez-Robles , Julián Fernández , Bruno Lomonte , Mahmood Sasa , Mónica Saldarriaga-Cordoba , Jaime Andrés Pereañez , Omayra Aguilera , Vitelbina Núñez-Rangel","doi":"10.1016/j.biochi.2025.06.003","DOIUrl":"10.1016/j.biochi.2025.06.003","url":null,"abstract":"<div><div>In the Americas, the genus <em>Micrurus</em> (coral snakes) includes the highest number of snake species, and Colombia is the second country with the greatest species diversity. <em>Micrurus dumerilii</em> has wide distribution and clinical importance in the country. The variability of its venom has not been extensively studied, and this could have implications for the neutralization by antivenoms. In this study, we explored the phylogenetic relationships between specimens from three regions of Colombia (Antioquia, Chocó, and Santander) and the variation in their venoms using proteomics, <em>in vitro</em> and <em>in vivo</em> assays, and assessment of antigenic recognition by the anticoral-INS antivenom. Phylogenetic analyses using <em>nd4</em>, <em>Cyt b</em>, and 16S rRNA gene fragments showed a close relationship between <em>M. dumerilii</em> from Ecuador and Chocó (Colombia), and within the <em>M. dumerilii</em> clade, a particularly close relationship between specimens from Antioquia and Santander. The venoms of <em>M. dumerilii</em> showed high overall similarity in their chromatographic profiles, with peaks corresponding to the three-finger toxin (3FTx) and phospholipase A<sub>2</sub> (PLA<sub>2</sub>) protein families being predominant. Some differences were observed in the number of protein families identified in each venom, but the main fraction responsible for lethality in the venoms from Antioquia, Chocó, and Santander was preserved. The commercial antivenom available in Colombia recognizes venom from all three regions. These general antigenic similarities between samples suggest that it may not be necessary to include <em>M. dumerilii</em> venoms from different geographic areas as immunogens for the production of antivenom against this species.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 93-105"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144277037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside front cover-EDB 内部前盖- edb

IF 3.3 3区生物学

Biochimie Pub Date : 2025-06-07 DOI: 10.1016/S0300-9084(25)00104-X

引用次数: 0