Applied Biochemistry and Microbiology最新文献

Lipoxygenase in Adaptation of the Neurospora crassa Cells to Temperature Stress 角叉菜神经孢子菌细胞适应温度胁迫过程中的脂氧合酶

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824604864

S. Yu. Filippovich, G. P. Bachurina

{"title":"Lipoxygenase in Adaptation of the Neurospora crassa Cells to Temperature Stress","authors":"S. Yu. Filippovich, G. P. Bachurina","doi":"10.1134/S0003683824604864","DOIUrl":"10.1134/S0003683824604864","url":null,"abstract":"The adaptation of the N. crassa lipoxygenase (LOX) in response to heat (45°C) and cold (4°C) shock was studied. The difference was revealed in the dynamics of the LOX activity depending on the growing conditions of the mycelium. After incubation of the surface culture at 45°C, a gradual increase in the specific activity of the enzyme was observed with maximum at 2–3 h, followed by a subsequent decrease to the initial level. Under the same conditions, in a submerged culture, a decrease in the LOX activity was observed after 5 min; however, after 1 h of incubation, the enzyme activity also reached the initial level. The sensitivity of the N. crassa LOX to elevated temperatures is very high, since it is noted only in a narrow temperature range: the effect was detected at 45oC; however, the enzymatic activity did not change in the culture incubated at 42°C, but, on the other hand, LOX was completely inactivated in the mycelium at 48°C. When the fungal cells were exposed to cold, the specific LOX activity increased after 1 h, then decreased to the initial level (2–3 h) and increased again, reaching a maximum after 18 h. When two stress factors, cold and starvation, acted simultaneously on the N. crassa cells, the treatment with cold had a decisive effect on the LOX activity, which was especially noticeable after 8 h of incubation.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1171 - 1176"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Growth and Metabolism of Lacticaseibacillus casei in Model Milk Media and Semi-Hard Cheeses

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824604980

G. M. Sviridenko, D. S. Vakhrusheva, O. M. Shukhalova, D. S. Mamykin, V. A. Mordvinova

引用次数: 0

Phylogenetic Composition of Microbial Communities from Fouling of Titanium Plates in the Coastal Zone of the Black and White Seas 黑海和白海沿岸地区钛板污垢微生物群落的系统发育组成

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605195

A. L. Bryukhanov, A. S. Shutova, K. A. Komarova, T. A. Semenova, A. A. Semenov, V. A. Karpov

{"title":"Phylogenetic Composition of Microbial Communities from Fouling of Titanium Plates in the Coastal Zone of the Black and White Seas","authors":"A. L. Bryukhanov, A. S. Shutova, K. A. Komarova, T. A. Semenova, A. A. Semenov, V. A. Karpov","doi":"10.1134/S0003683824605195","DOIUrl":"10.1134/S0003683824605195","url":null,"abstract":"With high-throughput sequencing of the variable region V3–V4 of the 16S rRNA gene, the study of the full phylogenetic composition of microbial communities developed on the surface of titanium plates exposed in the water column of the coastal zone of the Black and White Seas was carried out. The presence of potentially corrosive microorganisms from various physiological groups, such as sulfate-reducing bacteria, acidophilic iron-oxidizing bacteria and archaea, sulfur-oxidizing and nitrifying bacteria, was shown in the fouling. In the fouling of titanium plates exposed in the Black Sea, the most common microorganisms were uncultivated sulfate-reducing bacteria of the order Desulfotomaculales, which accounted for 8.13% of all 16S rRNA gene sequence reads, as well as acidophilic iron-oxidizing bacteria of the genera Acidiferrobacter (5.47%), Acidithiobacillus (4.52%), and Acidiphilium (2.55%). Acidophilic archaea accounted for up to 7.97% of all reads. In the fouling of titanium plates exposed in the White Sea, the most common were also acidophilic bacteria from the orders Acidiferrobacterales and Acidithiobacillales (7.68%), as well as acidophilic archaea from the order Thermoplasmatales (7.43%). Uncultivated sulfate-reducing bacteria of the order Desulfotomaculales were also represented in relatively high numbers (6.61% of all reads).","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1259 - 1265"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome Analysis of the Nitric Oxide Donor Effect on Pisum Sativum L. Roots

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824604694

A. M. Egorova, I. A. Tarchevsky

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Biosynthesis of Citric Acid from Glucose–Fructose Syrups by the Yeast Yarrowia lipolitica 脂肪酵母菌从葡萄糖果糖浆中生物合成柠檬酸的过程

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824604074

S. V. Kamzolova, V. A. Samoylenko, R. K. Allayarov, A. A. Mironov, Y. N. Lunina, N. E. Vorobyeva, A. A. Shaikin, M. B. Vainshtein

引用次数: 0

Entomopathogenic Fungi of the Genus Akanthomyces and Closely Related Species as Antagonists of Phytopathogenic Microorganisms 作为植物病原微生物拮抗剂的赤霉属昆虫病原真菌和近缘种

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824604852

A. A. Choglokova, G. V. Mitina, M. A. Cherepanova

引用次数: 0

Modern Methods for the Isolation and Identification of Peptaibols from Filamentos Fungi

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605547

V. V. Sokolov, A. E. Kuvarina, V. S. Sadykova

引用次数: 0

Erratum to: The Effect of Space Flight Factors on the Interaction of Escherichia coli with Bacteriophage T7

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824030025

N. N. Sykilinda, A. A. Lukyanova, V. V. Lavrikova, I. V. Kutnik, N. V. Panin, N. A. Staritsyn, K. A. Miroshnikov

引用次数: 0

Comparison of Genomes, Resistomes, and Antimicrobial Properties of the Three Lactococcus lactis Strains from Fermented Milk and Cereal Products of South Africa and Russia 比较南非和俄罗斯发酵乳和谷物产品中三种乳球菌菌株的基因组、抗性组和抗菌特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824606012

K. V. Moiseenko, O. A. Glazunova, O. S. Savinova, T. V. Fedorova

{"title":"Comparison of Genomes, Resistomes, and Antimicrobial Properties of the Three Lactococcus lactis Strains from Fermented Milk and Cereal Products of South Africa and Russia","authors":"K. V. Moiseenko, O. A. Glazunova, O. S. Savinova, T. V. Fedorova","doi":"10.1134/S0003683824606012","DOIUrl":"10.1134/S0003683824606012","url":null,"abstract":"Whole-genome sequencing was carried out, and the main characteristics of the genomes of three new strains of L. lactis AM1, MA1, and dlA, isolated from fermented milk and cereal grain products of South Africa and Russia, were established. The dual approach of in vitro and in silico analysis used in this work allowed us to assess more fully the bacteriocinogenic potential and antibiotic resistance of L. lactis strains. It was shown that L. lactis AM1 and dlA had an antagonistic effect against the test cultures of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538. The growth retardation of E. coli and S. aureus after 48 hours of cocultivation was 65–75 and 30–50%, respectively. The L. lactis MA1 strain did not exhibit a significant antagonistic effect; the growth inhibition of E. coli and S. aureus was 13 and 26%, respectively. According to the results of in silico analysis using the BAGELv4.0 service, gene clusters of class IId lactococcin-like bacteriocins were found in the genomes of all three strains. In the genomes of the dlA and AM1 strains, the sactipeptide cluster, a class I bacteriocin, is additionally annotated. In silico analysis of the L. lactis genomes showed the absence of transmissible antibiotic resistance genes, and all three strains showed a phenotype susceptible to the main classes of clinically relevant antibiotics (ampicillin, streptomycin, kanamycin, gentamicin, erythromycin, clindamycin, vancomycin, tetracycline, and chloramphenicol). Taken together, the results provide valuable information about the intraspecific diversity of L. lactis and indicate the promise of using new strains as starter cultures and biopreservatives.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1223 - 1239"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683824606012.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: Development of Immunoassay for Detection of Engineered Endolysin LysAm24-SMAP

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824030013

A. A. Klimova, I. V. Grigoriev, D. V. Vasina, M. N. Anurova, V. A. Gushchin, N. P. Antonova

引用次数: 0