Applied Biochemistry and Microbiology最新文献

New Peculiarities in the Virulence Factors of Pectobacterium atrosepticum 无核果胶杆菌毒性因子的新特征

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824605213

L. A. Lomovatskaya, A. M. Goncharova

{"title":"New Peculiarities in the Virulence Factors of Pectobacterium atrosepticum","authors":"L. A. Lomovatskaya, A. M. Goncharova","doi":"10.1134/S0003683824605213","DOIUrl":"10.1134/S0003683824605213","url":null,"abstract":"Nine strains of Pectobacterium atrosepticum (Pca) were analyzed in this work, and new information about the pathogen properties and regulation of its main virulence factors was obtained. In exopolysaccharides (EPS) of Pca, anionic groups (({text{PO}}_{4}^{{3 - }})) were detected, the amount of which varied in different strains and probably contributed to the formation of denser biofilms. Most Pca strains inhibited to varying degrees the growth of potato plants in vitro; however, one of them (426) activated significantly the growth of the plants of the resistant potato variety Lugovskoi. Based on the totality of the disease symptoms (chlorosis, necrosis, wilt, inhibition of growth rate), all P. atrosepticum strains were divided by the degree of virulence. In addition, it was demonstrated that the incubation of bacteria with homogenate from potato plants in vitro modulated the activity of pectinase in Pca homogenate from the plants of the resistant potato variety Lugovskoi inhibited the activity of exopectinase, while that from the susceptible variety Lukyanovskii activated it. At the same time, the activity of exopectinase from the avirulent strain 426 was inhibited to the greatest extent. It is assumed that this was a reason for the stimulation of the growth of the resistant potato variety plants in vitro.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"194 - 199"},"PeriodicalIF":1.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytic Properties of Immobilized Phytase of Silvania hatchlandensis FG 3.9.1 Silvania hatchlandensis FG 3.9.1 固定化植酸酶的催化特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824606632

K. G. Semenova, Yu. G. Maksimova

{"title":"Catalytic Properties of Immobilized Phytase of Silvania hatchlandensis FG 3.9.1","authors":"K. G. Semenova, Yu. G. Maksimova","doi":"10.1134/S0003683824606632","DOIUrl":"10.1134/S0003683824606632","url":null,"abstract":"A phytase preparation was obtained from the cells of haloalkalitolerant bacteria identified as Silvania hatchlandensis isolated from the soil of a soda sludge storage facility. When the enzyme was immobilized in barium alginate gel and cross-linked with activated chitosan, 97 and 95% of the native protein activity, respectively, was retained. It was shown that 70% of the phytase activity was retained when using the enzyme immobilized in alginate and bound to chitosan over six consecutive reaction cycles. Immobilization resulted in an insignificant decrease in the maximum reaction rate and a decrease in the Michaelis constant. Immobilized phytase was more thermally stable compared to the free form of the enzyme: the thermal inactivation constant of the immobilized enzyme at 70°C decreased by 1.1–1.2 times. The immobilized enzyme retained activity at pH 3–12; the pH optimum of the enzyme after immobilization did not change and was equal to 5.0. The specific activity of the enzyme covalently attached to activated chitosan is higher than that of the native enzyme in low and high pH media. Immobilized phytase of haloalkalitolerant Silvania hatchlandensis can be used in feed production and other areas of agriculture.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"84 - 91"},"PeriodicalIF":1.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Method for Analyzing the Antimicrobial Activity of Peptides via the Escherichia coli Expression System

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824606292

E. N. Grafskaia, D. D. Kharlampieva, P. A. Bobrovsky, M. Y. Serebrennikova, V. N. Lazarev, V. A. Manuvera

{"title":"Method for Analyzing the Antimicrobial Activity of Peptides via the Escherichia coli Expression System","authors":"E. N. Grafskaia, D. D. Kharlampieva, P. A. Bobrovsky, M. Y. Serebrennikova, V. N. Lazarev, V. A. Manuvera","doi":"10.1134/S0003683824606292","DOIUrl":"10.1134/S0003683824606292","url":null,"abstract":"The test system for an assay of new potential antimicrobial peptides (AMP) based on the expression of recombinant AMP-encoding genes in Escherichia coli cells has been proposed. This approach has a number of advantages over the use of chemically synthesized peptides, and both proposed methods effectively complement each other. Our approach does not impose limitations on the AMP size, facilitates high-throughput screening of mutant plasmid libraries, and has lower cost and complexity compared to the use of synthetic peptides. The core of our methodology involves transformation of the model Gram-negative bacterium E. coli with plasmids carrying a recombinant AMP-encoding gene regulated by an inducible promoter. Following transcription induction, bacteria synthesize the AMP, which ultimately leads to cell death. The assessment of bacterial growth is carried out either by measuring the optical density of a bacterial culture grown in liquid media in a microplate or by spot plating of bacterial culture serial dilutions on an solid agar medium.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"76 - 83"},"PeriodicalIF":1.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the Causal Agent of Grapevine Downy Mildew Plasmopara viticola Based on Quantitative PCR

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824604189

N. N. Nityagovsky, A. A. Dneprovskaya, A. A. Ananev, K. V. Kiselev, O. A. Aleynova

引用次数: 0

Rhodococcus erythropolis А-27 as a Biocatalyst for Enantioselective Reduction of Ketones in the Presence of High Concentrations of Isopropanol 红球菌 А-27 作为生物催化剂在高浓度异丙醇存在下对映体选择性还原酮体

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824606085

N. I. Petukhova, A. V. Mityagina, V. V. Zorin

引用次数: 0

Biologically Active Quinolinone Alkaloid from the Marine Fungus Penicillium polonicum KMM 4719

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824605912

S. S. Starnovskaya, N. N. Kirichuk, V. E. Chausova, U. V. Khudyakova, E. A. Chingizova, A. R. Chingizov, A. N. Yurchenko, E. A. Yurchenko

引用次数: 0

Phytostimulating Activity of Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 and Its groEL2 Gene Knockout Mutant

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824605924

N. V. Agafonova, G. A. Ekimova, Y. E. Firsova, M. L. Torgonskaya

{"title":"Phytostimulating Activity of Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 and Its groEL2 Gene Knockout Mutant","authors":"N. V. Agafonova, G. A. Ekimova, Y. E. Firsova, M. L. Torgonskaya","doi":"10.1134/S0003683824605924","DOIUrl":"10.1134/S0003683824605924","url":null,"abstract":"For the first time, the genome of the dichloromethane destructor Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 was analyzed for the presence of genetic determinants indicating its potential as a plant growth stimulator, and the ability of this strain and its groEL2 gene mutant to improve plant growth was determined. The genome of strain DM4 contains genes involved in the biosynthesis of phytohormones (indolyl-3-acetic acid and cytokinins), siderophores, carotenoids, poly-β-hydroxybutyrate, hydrolytic enzymes, as well as enzymes involved in the degradation of D-cysteine, protection from UV-damage, and phosphate solubilization. Inoculation of lettuce sprouts by strain DM4 had a positive effect on plant growth and development and increased adaptive defense and resistance to short-term temperature stress in plant growth experiments. Comparative analysis of the production of auxins, siderophores, hydrolytic enzymes, D-cysteine desulfohydrase activity, and the ability to solubilize insoluble phosphates in strains DM4 and DM4 ΔgroEL2 showed that disruption of the groEL2 gene led to a decrease in the synthesis of indole derivatives and phosphate solubilizing ability in the mutant. Assessment of the impact of inoculation of lettuce plants by these strains also demonstrated a decrease in the phytostimulating potential of DM4 ΔgroEL2 compared to the original strain. The data obtained indicate that the chaperonin GroEL2 in M. dichloromethanicum subsp. dichloromethanicum DM4 indirectly affects its phytostimulating activity.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"173 - 183"},"PeriodicalIF":1.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bioavailable Nanocomposition of Chitosan–Copper Nanoparticles as an Alternative to Antibiotics in Broiler Feed

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824604591

K. V. Apryatina, I. A. Egorov, S. D. Zaitsev, G. Yu. Laptev, E. V. Salomatina, L. A. Smirnova, A. G. Samodelkin, V. G. Frolov

{"title":"A Bioavailable Nanocomposition of Chitosan–Copper Nanoparticles as an Alternative to Antibiotics in Broiler Feed","authors":"K. V. Apryatina, I. A. Egorov, S. D. Zaitsev, G. Yu. Laptev, E. V. Salomatina, L. A. Smirnova, A. G. Samodelkin, V. G. Frolov","doi":"10.1134/S0003683824604591","DOIUrl":"10.1134/S0003683824604591","url":null,"abstract":"Pathogens pose a serious threat to agriculture as they cause diseases, reduce the growth rate and efficiency of farm birds and animals and causing economic losses. Therefore, antibiotics continue to be used to combat pathogens and for prevention, despite all their negative effects and the bacterial resistance to them. Effective alternatives are needed that exclude the use of vaccines and drugs. An aggregatively stable nanocomposition of chitosan and nanoparticles of copper with an average size of the latter of 25–30 nm was developed. The bactericidal effect of the nanocomposition was shown in vitro on the pathogenic bacteria Enterococcus faecalis and investigated in vivo in the composition of the drink and feed of the broiler chickens in comparison with the antibiotic “Maxus,” which is used in their diet, on a wide range of pathogenic microorganisms. It was shown that the number of bacteria in broilers was 1.88% when the nanocomposition was administered, which is more than two times less compared to the group in which the antibiotic was used.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"125 - 136"},"PeriodicalIF":1.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Agronomically Valuable Strains of the Genus Streptomyces and Their Biochemical Characteristics 链霉菌属有农用价值的新菌株及其生化特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-04-07 DOI: 10.1134/S0003683824605110

I. G. Shirokikh, Ya. I. Nazarova, N. A. Bokov, A. A. Alalykin, A. A. Shirokikh

{"title":"New Agronomically Valuable Strains of the Genus Streptomyces and Their Biochemical Characteristics","authors":"I. G. Shirokikh, Ya. I. Nazarova, N. A. Bokov, A. A. Alalykin, A. A. Shirokikh","doi":"10.1134/S0003683824605110","DOIUrl":"10.1134/S0003683824605110","url":null,"abstract":"Organic farming is a global trend that increases the demand for biological preparations for use in agricultural production. This paper characterizes new actinomycete isolates from soil samples taken in various agrocenoses of the Vyatka–Kama Urals. As a result of preliminary testing (about 350 strains), strains 8Al3, N27-25, and P15-2 were isolated on the basis of antifungal activity. The cultures were identified by 16S rRNA as bacteria of the genus Streptomyces. An active substance with antifungal effect—scopafungin—was identified using HPLC-MS/MS. Along with the inhibitory effect on phytopathogenic fungi, these strains produce auxins in the presence of 100 μg/mL of L-tryptophan (17.4–20.8 μg/mL), have cellulolytic activity, and have a stimulating effect on germination and accumulation of the dry biomass by wheat, clover, and mustard seedlings. In possessing a complex of properties beneficial for plants, Streptomyces strains 8Al3, N27-25, and P15-2 can be used as candidates for the creation of biological preparations with fungicidal and phytostimulating effects.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"184 - 193"},"PeriodicalIF":1.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Rate of Sorbitol Metabolism Negatively Affects Methanol-Induced Synthesis of Recombinant Enzymes in the Yeast Komagataella kurtzmanii and Komagataella phaffii

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824090011

P. I. Akentyev, D. D. Sokolova, I. I. Gubaidullin, S. V. Rykov, O. V. Berezina, N. O. Chernomorova, E. N. Elpidina, D. G. Kozlov

{"title":"Increased Rate of Sorbitol Metabolism Negatively Affects Methanol-Induced Synthesis of Recombinant Enzymes in the Yeast Komagataella kurtzmanii and Komagataella phaffii","authors":"P. I. Akentyev, D. D. Sokolova, I. I. Gubaidullin, S. V. Rykov, O. V. Berezina, N. O. Chernomorova, E. N. Elpidina, D. G. Kozlov","doi":"10.1134/S0003683824090011","DOIUrl":"10.1134/S0003683824090011","url":null,"abstract":"It is known that sorbitol metabolism in the yeast K. kurtzmanii and K. phaffii is limited by the level of sorbitol dehydrogenase expression. In the present work, we obtained transformants of K. kurtzmanii and K. phaffii that synthesized the thermostable endoglucanase Egh12 of Thielavia terrestris, xyloglucanase Xgh12b of Aspergillus cervinus, and cathepsin-L CathL of Tribolium castaneum under the control of the methanol-inducible promoter PAOX1 and had different rates of sorbitol metabolism due to additional expression of the sorbitol dehydrogenase gene. Synthesis and secretion of the target enzymes were investigated on media containing methanol and sorbitol in different ratios. The results obtained showed that an increase in the rate of sorbitol metabolism in the producers had a negative effect on the synthesis of target proteins up to complete inhibition of their production. At the same time, low-efficiency sorbitol assimilation by these strains, limited by the expression of sorbitol dehydrogenase or by the concentration of sorbitol in the medium, promoted an increase in the secretion of Egh12 and increased enzyme production up to 30% by stimulating the growth of the cell biomass.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1648 - 1657"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0