Applied Biochemistry and Microbiology最新文献

Development of Immunoassay for Detection of Engineered Endolysin LysAm24-SMAP 开发检测工程溶解素 LysAm24-SMAP 的免疫测定方法

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604384

A. A. Klimova, I. V. Grigoriev, D. V. Vasina, M. N. Anurova, V. A. Gushchin, N. P. Antonova

{"title":"Development of Immunoassay for Detection of Engineered Endolysin LysAm24-SMAP","authors":"A. A. Klimova, I. V. Grigoriev, D. V. Vasina, M. N. Anurova, V. A. Gushchin, N. P. Antonova","doi":"10.1134/s0003683824604384","DOIUrl":"https://doi.org/10.1134/s0003683824604384","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>In recent years modified bacteriophage lysins have widely been investigated for the purposes of development of antibacterial therapy. Thus, effective and precise methods for the quantitative analysis of these enzymes are in high demand. The enzyme-linked immunosorbent assay (ELISA) method has been developed for the detection of recombinant modified endolysin LysAm24-SMAP in biological samples. The optimal parameters for protein detection were determined, in particular, the influence of salt and the composition of the buffer system for preparation of the samples was studied. The applicability of the immunodetection system of the genetically engineered endolysin LysAm24-SMAP in various biological samples with enzyme concentrations from 0.4 ng/mL was demonstrated. In addition, the influence of matrix effects in samples of animal organs and tissue homogenates and producer strain lysates and their individual components during the analysis was assessed and it was shown that 0.65 M NaCl addition in the ELISA buffer is crucial for achieving correct results and reduces nonspecific interactions in the case of LysAm24-SMAP. The effectiveness of the developed system in the immunochemical control of the bacteriolytic enzyme was confirmed.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of the Pseudomonas sp. OBA 2.4.1 Strain for Presowing Treatment of Pea Seeds (Pisum Sativum L.) in the Presence of Heavy Metals and Glyphosate 利用假单胞菌 OBA 2.4.1 菌株在重金属和草甘膦存在的情况下对豌豆种子（Pisum Sativum L.）进行播前处理

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604414

L. R. Khakimova, O. V. Chubukova, Z. R. Vershinina

{"title":"Use of the Pseudomonas sp. OBA 2.4.1 Strain for Presowing Treatment of Pea Seeds (Pisum Sativum L.) in the Presence of Heavy Metals and Glyphosate","authors":"L. R. Khakimova, O. V. Chubukova, Z. R. Vershinina","doi":"10.1134/s0003683824604414","DOIUrl":"https://doi.org/10.1134/s0003683824604414","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000Abstract—</h3>The results of study of the effects of the Pseudomonas sp. OBA 2.4.1 PGPB strain resistant to NiCl2 (up to 3 mM), Pb(CH3COO)2 (up to 5 mM), and glyphosate (up to 8 mg/mL) on Pisum sativum L. plants at different concentrations of the heavy metals and the herbicide are reported. It was found that this strain had a positive effect on the root length of pea seedlings in the presence of heavy metals indicating an increase in the plant resistance to nickel and lead stress. However, no such effect was observed in the experiment with glyphosate, which confirmed the high toxicity of this compound. The results obtained demonstrated that the Pseudomonas sp. OBA 2.4.1 strain promoted the growth of Pisum sativum L. under nickel and lead stress; these findings can further be used for the development of complex biopreparations intended both for the protection of agricultural plants from the effects of heavy metals and for remediation of contaminated soils.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Yersinia pestis Antigens in the Reception of Plague Diagnostic Bacteriophage L-413C 鼠疫耶尔森菌抗原在接收鼠疫诊断噬菌体 L-413C 中的作用

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604438

A. A. Byvalov, L. G. Dudina, T. B. Kravchenko, S. A. Ivanov, I. V. Konyshev, N. A. Morozova, A. V. Chernyadiev, S. V. Dentovskaya

{"title":"The Role of Yersinia pestis Antigens in the Reception of Plague Diagnostic Bacteriophage L-413C","authors":"A. A. Byvalov, L. G. Dudina, T. B. Kravchenko, S. A. Ivanov, I. V. Konyshev, N. A. Morozova, A. V. Chernyadiev, S. V. Dentovskaya","doi":"10.1134/s0003683824604438","DOIUrl":"https://doi.org/10.1134/s0003683824604438","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The role of surface antigens of Yersinia pestis in reception of the phage L-413C was evaluated experimentally. Based on the methods of phage inactivation after its co-incubation with soluble or bead-bounded antigens, the importance of the plague microbe LPS in the phage reception was confirmed, and the inability to bind the capsular antigen F1, Ail protein, and two autotransporters YapF and YapM was shown. The native and recombinant PsaA, being solved, significantly inhibited the lytic activity of the phage in contrast to the bead-bound antigens. The knockout EV cells (ΔpsaA) are able to bind the phage particles as well as the wild strain. The use of three methods to evaluate the role of the PsaA antigen in phage L-413C reception gave contradictory results. On the one hand, the reactive domains of PsaA are able to interact with phage particles in solution. At the same time, these domains appear to determine nonspecific binding of the PsaA protein to the underlying bacterial cell structures or polystyrene microsphere, preventing phage adhesion.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the Biosynthesis of Butyric Acid from Glucose through the Inverted Fatty Acid β-Oxidation Pathway by Recombinant Escherichia coli Strains 重组大肠杆菌菌株通过倒置脂肪酸 β-氧化途径从葡萄糖优化丁酸的生物合成

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604360

A. Yu. Gulevich, A. Yu. Skorokhodova, V. G. Debabov

{"title":"Optimization of the Biosynthesis of Butyric Acid from Glucose through the Inverted Fatty Acid β-Oxidation Pathway by Recombinant Escherichia coli Strains","authors":"A. Yu. Gulevich, A. Yu. Skorokhodova, V. G. Debabov","doi":"10.1134/s0003683824604360","DOIUrl":"https://doi.org/10.1134/s0003683824604360","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The biosynthesis of butyric acid from glucose through inverted fatty acid β-oxidation by recombinant Escherichia coli strains was optimized. An increased yield of the target compound was achieved resulting from the plasmid expression of the atoB, fadB, and fadE/fabI genes in the core strain MG∆4 PL-tesB ΔyciA (MG1655 ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDϕ10-tesB, ∆yciA). The positive effect of enforced ATP hydrolysis on microaerobic conversion of the carbohydrate substrate to the final product by the recombinants was demonstrated. Activation of the futile cycle of pyruvate–phosphoenolpyruvate–pyruvate, due to the increased expression of the ppsA gene, ensured a marked increase in glucose consumption by the recombinants and led to an increase in the molar yield of butyric acid up to 39.5%. When the components of the H+-ATP synthase complex were uncoupled resulting from the deletion of atpFH genes, the molar yield of butyric acid from glucose demonstrated by the strain forming butyryl-CoA by the action of enoyl-ACP reductase FabI reached 46%.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platinum Nanoparticles in Aqueous Solutions of a Chitosan–Vinylpyrrolidone Copolymer: Synthesis and Biological Activity 壳聚糖-乙烯基吡咯烷酮共聚物水溶液中的铂纳米粒子：合成与生物活性

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604451

D. N. Zuev, E. I. Cherkasova, K. V. Apryatina, S. D. Zaitsev, L. A. Smirnova

引用次数: 0

Bacterial Expression of Crm197: Investigation and Optimization of Gene Expression Factors for Effective Production in E. coli Crm197 的细菌表达：调查和优化基因表达因子以在大肠杆菌中有效生产

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s000368382460444x

S. O. Rogozhkin, A. S. Gerasimov

{"title":"Bacterial Expression of Crm197: Investigation and Optimization of Gene Expression Factors for Effective Production in E. coli","authors":"S. O. Rogozhkin, A. S. Gerasimov","doi":"10.1134/s000368382460444x","DOIUrl":"https://doi.org/10.1134/s000368382460444x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>CRM197 (Cross Reacting Material 197) is an inactive form of the C. diphtheriae exotoxin used as a carrier protein for the development and production of conjugated polysaccharide vaccines and immunotherapeutic drugs. However, the development of these research areas is not possible without an efficient and cost-effective technology to produce CRM197 of the proper quality. In this study, we developed a highly efficient method to produce recombinant CRM197 as a fusion with SUMO protein, yielding more than three grams per liter in the form of inclusion bodies. We examined the significant effect of the type of expression vector, the heterologous gene expression conditions, and cultivation on its solubility. Using a combination of reduced cultivation temperature and the promoter of the gene encoding the heat shock protein CspA, we achieved an increase in the solubility level of SUMO-CRM197 of more than 30%, with an overall biosynthesis level of more than two grams per liter. Coexpression of the target gene with the DsbC disulfide isomerase gene allowed us to obtain the target protein completely in the soluble state with a yield of more than 1.4 grams per liter. The results obtained may become the basis for the development of a promising domestic technology for the production of CRM197.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeny and Characterization of Endophytic Bacteria Isolated from the Rhizosphere of Pea Seedlings (Pisum Sativum L.) 从豌豆苗（Pisum Sativum L.）根瘤中分离出的内生细菌的系统发育和特征描述

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604426

L. E. Makarova, Yu. A. Markova, Yu. V. Zaytseva, A. A. Bychkova, I. V. Gorbenko, Yu. M. Konstantinov, I. A. Vasiliev, A. S. Morits, P. A. Bizikov

{"title":"Phylogeny and Characterization of Endophytic Bacteria Isolated from the Rhizosphere of Pea Seedlings (Pisum Sativum L.)","authors":"L. E. Makarova, Yu. A. Markova, Yu. V. Zaytseva, A. A. Bychkova, I. V. Gorbenko, Yu. M. Konstantinov, I. A. Vasiliev, A. S. Morits, P. A. Bizikov","doi":"10.1134/s0003683824604426","DOIUrl":"https://doi.org/10.1134/s0003683824604426","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Previously it was shown that endophytic bacteria had the ability to move out of the roots of pea plant seedlings (Pisum sativum L.) into the rhizosphere. In this study, six distinct bacterial strains were isolated from the root growth medium during the cultivation of seedlings in an aqueous medium. By analyzing the nucleotide sequence of 16S rRNA genes, the taxonomic position of these strains was established. Their morphological and cultural parameters were assessed, and the activity of hydrolytic enzymes (pectinase, cellulase, protease) and the IAA-producing capability were examined. It has been observed that the quantity of endophytic bacteria that appears on the root surface during the growth of pea seedlings significantly surpasses the quantity present in the root tissues. It is assumed that hydrolytic enzymes such as pectinase and cellulase are involved in the release of bacteria into the external environment, causing the destruction of carbohydrate structures in plant cell walls. The metabolic parameters established in the studied strains, and the significance of these endophytic bacteria for the host plant after their exit from the roots into the rhizosphere are under discussion.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Organic Solvents on the Results of Immunoenzyme Determination of Herbicide Butachlor: Selection of Sample Preparation Modes 有机溶剂对除草剂丁草胺免疫酶测定结果的影响：样品制备模式的选择

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604359

A. N. Berlina, N. I. Smirnova, N. S. Komova, K. V. Serebrennikova, A. V. Zherdev, B. B. Dzantiev

{"title":"Influence of Organic Solvents on the Results of Immunoenzyme Determination of Herbicide Butachlor: Selection of Sample Preparation Modes","authors":"A. N. Berlina, N. I. Smirnova, N. S. Komova, K. V. Serebrennikova, A. V. Zherdev, B. B. Dzantiev","doi":"10.1134/s0003683824604359","DOIUrl":"https://doi.org/10.1134/s0003683824604359","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The influence of organic solvents such as methanol and acetonitrile on the results of butachlor immunoassay in samples of rice and rice products was studied. The schemes of enzyme immunoassay using: (a) native antiserum containing specific antibodies to butachlor and antispecies antibodies labeled with horseradish peroxidase, and (b) biotinylated specific antibodies and streptavidin labeled with peroxidase are considered. The close values of IC10 (0.77 and 0.68 ng/mL, respectively) and working range (2.6–165 and 2.4–192 ng/mL, respectively) were established for the two schemes, when analyzing in a mixture of phosphate buffer and methanol in a percentage ratio of 85 : 15, respectively. For the second scheme, the detection of butachlor in butachlor in rice-containing products is shown at 80-132% of the added analyte. A comparison of sample preparation methods allows us to recommend: (a) extraction of butachlor with hexane and re-dissolution of the dry residue in a buffer with 15% methanol or (b) preparation of a methanol extract followed by 6-fold dilution with a buffer solution.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Various Domestically Produced Proteolytic Enzyme Preparations on the Organoleptic Properties of Pea Protein Isolates 各种国产蛋白水解酶制剂对豌豆蛋白分离物感官特性的影响

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604335

I. V. Kravchenko, V. A. Furalyov, E. S. Pshennikova, E. V. Kostyleva, A. S. Sereda, E. I. Kurbatova, N. V. Tsurikova, A. N. Fedorov, V. O. Popov

引用次数: 0

The Dynamics of Cytoplasmic HSP70 and Chloroplast HSP70B Chaperone Levels under Heat Stress Differs in Three Pumpkin Species with Different Stress Resistance 热胁迫下细胞质 HSP70 和叶绿体 HSP70B伴侣蛋白水平的动态变化在三种抗胁迫能力不同的南瓜品种中存在差异

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-07-27 DOI: 10.1134/s0003683824604372

N. D. Murtazina, L. S. Sharapova, N. P. Yurina

{"title":"The Dynamics of Cytoplasmic HSP70 and Chloroplast HSP70B Chaperone Levels under Heat Stress Differs in Three Pumpkin Species with Different Stress Resistance","authors":"N. D. Murtazina, L. S. Sharapova, N. P. Yurina","doi":"10.1134/s0003683824604372","DOIUrl":"https://doi.org/10.1134/s0003683824604372","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The chaperone system of the cell is the first line of defense in plants under stress. In the present work, the effect of heat stress on the levels of cytoplasmic HSP70 and chloroplast HSP70B chaperones in three Cucurbita species (C. maxima Duchesne, C. pepo L., and C. moschata, Duchesne) differing in their stress resistance was studied. A correlation between the levels of cytoplasmic HSP70 and chloroplast HSP70B chaperones and pumpkin species under heat stress was demonstrated. Under stress, a significant increase in the chaperone levels was observed in the cells of the C. maxima pumpkin, namely, the level of the cytoplasmic HSP70 increased by 3.6 times, and the level of chloroplast HSP70B increased by two times. Heat stress caused a 1.7-fold increase in the level of the cytoplasmic chaperone HSP70 in the cells of the C. pepo pumpkin, while no significant change in the level of the HSP70B protein was observed. However, heat stress led to a decrease in the levels of both HSP70 and HSP70B compared to untreated plants in the C. moschata pumpkin. The dynamics of changes in the levels of cytoplasmic and chloroplast chaperones under heat stress is similar. It should be noted that the constitutive levels of HSP70 and HSP70B under normal conditions are higher in C. moschata and C. pepo compared to C. maxima. The analysis of the obtained data revealed an interesting pattern: high constitutive levels of HSPs result in an insignificant induction of HSPs, and, vice versa, low constitutive levels of these proteins correlate with the high induction rate of these proteins after exposure to heat stress. These findings are important in understanding the mechanisms of plant stress resistance and may be useful for selection and development of highly resistant productive varieties of agriculturally important plants.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0