K. K. Pirniyazov, D. I. Asrakulova, S. Sh. Rashidova
{"title":"Synthesis and Antimicrobial Properties of Chitosan Nanoascorbate of Bombyx Mori with Aluminum Ions","authors":"K. K. Pirniyazov, D. I. Asrakulova, S. Sh. Rashidova","doi":"10.1134/S0003683825602483","DOIUrl":"10.1134/S0003683825602483","url":null,"abstract":"<p>This work describes the synthesis of the chitosan nanoscorbate complex from <i>Bombyx mori</i> with aluminum ions based on chitosan and ascorbic acid using the ionotropic gelation method. Their physicochemical and antimicrobial properties against <i>Fusarium oxysporum</i>, which causes diseases in agricultural crops, are studied. The obtained chitosan nanoscorbate with aluminum ions has a larger inhibition zone compared to all variants against <i>Fusarium oxysporum</i>, which allows its use for presowing seed treatment with the component ratio of CS : AA : Al of 4 : 1 : 0.5.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"247 - 257"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. V. Popova, N. S. Domnina, I. I. Novikova, N. M. Kovalenko, I. L. Krasnobaeva
{"title":"The Effect of Chitosan and Chitosan Nanoparticles on Resistance of Wheat Plants to Dark Brown Spot","authors":"E. V. Popova, N. S. Domnina, I. I. Novikova, N. M. Kovalenko, I. L. Krasnobaeva","doi":"10.1134/S0003683825602422","DOIUrl":"10.1134/S0003683825602422","url":null,"abstract":"<p>A comparative study of fungistatic and elicitor activity of chitosan solutions and dispersions of chitosan nanoparticles obtained by fractional precipitation at pH 5.0 (NC-5) and pH 7.0 (NC-7) from chitosan with a molecular weight of 60 kDa was carried out. Both dispersions demonstrated higher fungistatic activity against <i>Bipolaris sorokiniana</i> compared to chitosan solution. A tendency towards a greater effect of inhibition of <i>B</i>. <i>sorokiniana</i> mycelium growth was revealed for NC-5 dispersion compared to NC-7 dispersion. It was found that pretreatment of wheat plants with NC-5 and NC-7 dispersions reduced leaf infestation with dark brown spot by three and two times, respectively, compared to the control. Chitosan solution under the same conditions suppressed disease development by only 10%. The studied dispersions can be considered as the basis for effective natural antimicrobial drugs with inducing activity.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"303 - 309"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Delic, K. L. Kolosov, I. A. Butorova, I. G. Andropova, E. A. Podorozhko, N. E. Benyaev, A. A. Artyukhov, A. N. Kuskov
{"title":"Macroporous Cryo-Structured Material Based on Vanillin-Cross-Linked Chitosan and Its Mechanical, Physicochemical, Sanitary-Chemical, and Antibacterial Properties","authors":"M. Delic, K. L. Kolosov, I. A. Butorova, I. G. Andropova, E. A. Podorozhko, N. E. Benyaev, A. A. Artyukhov, A. N. Kuskov","doi":"10.1134/S0003683825602562","DOIUrl":"10.1134/S0003683825602562","url":null,"abstract":"<p>This paper proposes a method for producing a macroporous cryostructured hydrogel material based on chitosan cross-linked with a low-toxicity agent, vanillin. The method involves a two-stage process: cryostructuring followed by freeze-drying and thermal cross-linking, resulting in the formation of Schiff bases. The resulting material is characterized by a developed system of interconnected pores with an average size of approximately 60 μm, a high swelling rate, and sufficient mechanical properties determined by its porous structure. The selective antibacterial activity of this material against <i>Staphylococcus aureus</i> (growth inhibition rate of 24.3%) was demonstrated, and its sanitary and chemical evaluation was also conducted. It was found that the material retains its key physical, mechanical, and sanitary properties after sterilization with ionizing radiation at doses of up to 25 kGy. The results demonstrate the potential of the developed material for use as a basis for biomedical products, such as wound dressings, hemostatic agents, and implants.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"210 - 219"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. R. Kildeeva, N. A. Sazhnev, M. G. Drozdova, E. A. Markvicheva, A. D. Ivanova, E. A. Svidchenko, N. M. Surin, V. I. Lozinsky
{"title":"Cryostructurates Based on Fibroin and Its Compositions with Chitosan: Preparation and Use as Macroporous Carriers for Cell Culturing","authors":"N. R. Kildeeva, N. A. Sazhnev, M. G. Drozdova, E. A. Markvicheva, A. D. Ivanova, E. A. Svidchenko, N. M. Surin, V. I. Lozinsky","doi":"10.1134/S000368382560232X","DOIUrl":"10.1134/S000368382560232X","url":null,"abstract":"<p>Regenerated fibroin isolated from natural silk and mixed solutions of it with chitosan were used to obtain cryostructurates—macroporous hydrogel matrices intended for application in tissue engineering as carriers for cell culturing. The conditions for stabilizing the structure of such biopolymeric carriers by cross-linking fibroin (Fb) and chitosan (Chit) with genipin (Gp) in alcohol solutions and the features of cell growth on the obtained materials were studied. Investigation of the interaction of genipin with fibroin and chitosan acetate showed that the reaction rate leading to the cross-linking of macromolecular chains of amino-containing biopolymers, as well as the concentration of the genipin conversion product, can be significantly increased compared to using the individual protein. With the use of confocal laser scanning microscopy, the structure of macroporous carriers was studied and their ability to support 3D growth of mouse fibroblasts (L929) in an in vitro model was demonstrated. The obtained biomaterials are promising for use in tissue engineering.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"198 - 209"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. G. Yarullina, E. A. Cherepanova, G. F. Burkhanova, E. A. Zaikina, V. O. Tsvetkov, A. V. Sorokan, I. S. Mardanshin, I. Y. Fatkullin, A. D. Pustovaya, J. N. Kalatskaja, K. S. Hileuskaya
{"title":"The Effect of Chitosan–Ag Nanocomposites on the Development of Protective Mechanisms in Potato Plants and Resistance to Phytophthora infestans","authors":"L. G. Yarullina, E. A. Cherepanova, G. F. Burkhanova, E. A. Zaikina, V. O. Tsvetkov, A. V. Sorokan, I. S. Mardanshin, I. Y. Fatkullin, A. D. Pustovaya, J. N. Kalatskaja, K. S. Hileuskaya","doi":"10.1134/S0003683825602690","DOIUrl":"10.1134/S0003683825602690","url":null,"abstract":"<p>A comparative assessment of the effect of two structurally different chitosan–silver nanocomposites (Ch–Ag 1 and Ch–Ag 2) on plant resistance to <i>Phytophthora infestans</i>, the state of the pro-/antioxidant system, the transcriptional activity of PR protein genes, and potato productivity was carried out. The dependence of the biological activity of Ch–Ag nanocomposites on their structure has been revealed. The mechanisms of activation of potato defense systems under the influence of Ch–Ag nanocomposites were mediated by the accumulation of hydrogen peroxide, a decrease in catalase activity, and an increase in the transcription of genes encoding PR proteins: chitinase (PR-3), thaumatin-like protein (PR-5), RNase (PR-10), and methyltransferase (MT). It was shown that treatment with Ch–Ag 2 reduced the degree of damage to the leaves of <i>P. infestans</i> and increased the mass of potato mini-tubers. The data obtained indicate that chitosan–silver nanocomposites are promising biological products for increasing the stability and productivity of potatoes.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"267 - 275"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Ay, H. İ. Guler, A. O. Belduz, M. Sahinkaya, O. Yıldız, S. Kolayli, O. Demircan
{"title":"Erratum to: Identification of Luteolin and Some Other Phenolics of Propolis as HIV-1-reverse Transcriptase Inhibitors","authors":"F. Ay, H. İ. Guler, A. O. Belduz, M. Sahinkaya, O. Yıldız, S. Kolayli, O. Demircan","doi":"10.1134/S0003683826010011","DOIUrl":"10.1134/S0003683826010011","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"400 - 400"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Antibacterial Activity of Biopolymer Films Based on Chitosan of Various Molecular Weights","authors":"V. V. Shchelkova, N. R. Kildeeva, I. A. Vasilenko","doi":"10.1134/S0003683825602847","DOIUrl":"10.1134/S0003683825602847","url":null,"abstract":"<p>In the context of growing antibiotic resistance, the possibility of creating non-toxic and biodegradable film coatings based on chitosan with modulated antibacterial properties is highly relevant. We have analyzed 270 samples of biopolymer films based on chitosan cross-linked with genipin with molecular weights of 60, 190, and 320 kDa. Suspensions of 30 clinical strains of <i>Staphilococcus aureus</i> (<i>n</i> = 10), <i>Escherichia coli</i> (<i>n</i> = 10), and <i>Candida albicans</i> (<i>n</i> = 10) microorganisms at a concentration of 10<sup>3</sup> CFU/mL have been used to evaluate the antibacterial activity of the biopolymer chitosan films (CF). Depending on the molecular weight of chitosan and the contact time of the biopolymer films with <i>S. aureus, E. coli, and C. albicans</i>, negative dynamics in the number of viable colonies have been revealed. These results suggest that chitosan-based biopolymers hold promise for the development of medical tools with programmable antibacterial and antifungal properties for practical medical use.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"220 - 226"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Obtaining and Study of Polyphenol and Chitosan Complexes","authors":"L. V. Shults, A. A. Krasnoshtanova","doi":"10.1134/S0003683825601787","DOIUrl":"10.1134/S0003683825601787","url":null,"abstract":"<p>The insoluble complexes formed by polyphenols from plant extracts and chitosan are of interest as potential delivery systems for polyphenols in the intestine. The complexes formed by polyphenols from an aqueous sage extract and chitosan have been investigated. These complexes have been shown to be stable in neutral media and to release polyphenols in highly acidic and highly alkaline environments. The complexes demonstrated significant polyphenol release in simulated intestinal fluid, with minimal release in simulated gastric and salivary fluids. It has been demonstrated that the complexes formed with chitosan are primarily composed of flavonoid glycosides and phenolic acids, with flavonoid aglycones present in trace amounts.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"227 - 232"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chitosan-Based Enzymatic Cell Wall Lysis Modulator for Bacterial DNA Isolation","authors":"K. Jasem, R. Z. Khayrullin, S. N. Kulikov","doi":"10.1134/S0003683825604573","DOIUrl":"10.1134/S0003683825604573","url":null,"abstract":"<p>The work has demonstrated that high-molecular chitosan enhances the lytic effect of lysostaphin on <i>Staphylococcus aureus</i> cells. More efficient lysis of bacterial cell walls by lysostaphin in the presence of chitosan made it possible to extract larger amounts of high molecular weight DNA suitable for sequencing from bacterial cells.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"318 - 322"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Influence of Chitosan Preparations on Spring Bread Wheat Development and Resistance to Diseases Caused by Biotrophic and Hemibiotrophic Fungi under Epidemic Conditions","authors":"L. Ya. Plotnikova, V. V. Knaub, V. V. Fomenko","doi":"10.1134/S0003683825604457","DOIUrl":"10.1134/S0003683825604457","url":null,"abstract":"<p>A comparative study of the effect of linear chitosan (Сh) and the globular form of chitosan, novochizol (Nc), on the growth and development of spring bread wheat, as well as protection against a complex of fungal diseases under epidemic conditions, was carried out. The indicators “Area Under the Disease Progress Curve” (AUDPC) and “Resistance Index” (RI) were calculated based on the observations. The research results showed that the Ch had greater growth-stimulating activity (on seed germination, stem and ear formation, and seed setting) and a protective effect against biotrophic and hemibiotrophic pathogens than Nc. The Nc–SA preparation (complex with 0.01% salisylic acid) showed the greatest protective effect, which increased in relation to the following diseases: leaf rust, stem rust, Septoria tritici blotch, and Septoria nodorum blotch. The increase in grain yield in the Ch-treated variants was mainly due to the growth-stimulating effects, while the Nc–SA treatment provided better protection against a range of diseases caused by the fungi with different feeding types.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"276 - 286"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}