Applied Biochemistry and Microbiology最新文献

The Role of Adenylate Cyclase and cAMP in Controlling the Virulence of Bacterial Pathogens in Animals, Phytopathogens, and Plant Mutualists 腺苷酸环化酶和cAMP在控制动物、植物病原体和植物共生菌细菌病原体毒力中的作用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825600307

L. A. Lomovatskaya, A. M. Goncharova

引用次数: 0

Low Temperatures Stimulate Alternative Splicing of the CPK26 Gene in Vitis amurensis Grapes 低温刺激葡萄CPK26基因的选择性剪接

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825600873

K. V. Kiselev, A. S. Dubrovina, Z. V. Ogneva, O. A. Aleynova

{"title":"Low Temperatures Stimulate Alternative Splicing of the CPK26 Gene in Vitis amurensis Grapes","authors":"K. V. Kiselev, A. S. Dubrovina, Z. V. Ogneva, O. A. Aleynova","doi":"10.1134/S0003683825600873","DOIUrl":"10.1134/S0003683825600873","url":null,"abstract":"Alternative splicing (AS) is a non-canonical gene splicing process that allows a single gene to synthesise multiple protein isoforms and enhance a variety of protein functions. In this study, the involvement of AS in the generation of plant resistance to abiotic stresses was investigated using the VaCPK26 calcium-dependent protein kinase (CPK) gene, which is responsible for the resistance of Vitis amurensis Rupr. grapes to soil salinity and drought. The level of VaCPK26 transcription in grape leaves was studied under the influence of different environmental factors. Under low temperature exposure, in addition to the full-length VaCPK26 transcript, a short-spliced VaCPK26s1 transcript was obtained that lacked the 2nd exon out of the 7 exons that make up the full-length VaCPK26. Recombinant VaCPK26 increased the resistance of grape cells to salt stress and drought, and overexpression of the spliced VaCPK26s1 transcript in V. amurensis grape cell cultures had no effect on resistance to the stresses tested. These results show that AS can lead to the loss of properties of spliced transcripts characteristic of the original full-length form, which is important for complete understanding of the biological functions of CPK and alternative splicing.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"987 - 995"},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Potential of the Micromycete Emericellopsis sp. E102 and the Influence of Cultivation Conditions on the Biosynthesis of Antibiotics emerellopsis sp. E102的抑菌潜力及培养条件对抗生素生物合成的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825601271

V. V. Sokolov, I. V. Mironov, A. Yu. Simonov, I. B. Levshin, M. L. Georgieva, V. S. Sadykova

{"title":"Antimicrobial Potential of the Micromycete Emericellopsis sp. E102 and the Influence of Cultivation Conditions on the Biosynthesis of Antibiotics","authors":"V. V. Sokolov, I. V. Mironov, A. Yu. Simonov, I. B. Levshin, M. L. Georgieva, V. S. Sadykova","doi":"10.1134/S0003683825601271","DOIUrl":"10.1134/S0003683825601271","url":null,"abstract":"In recent decades, the interest in Emericellopsis genus as producers of bioactive molecules has increased significantly due to the isolation of new compounds with potential pharmaceutical applications. Evaluation of the spectrum of antibiotic activity has allowed us to choose a promising producer of a new antibacterial compound, the strain Emericellopsis sp. E102 derived from saline soils. Strain E102, based on molecular and phylogenetic constructions, is allocated to a separate clade within the marine clade of Emericellopsis and is presumably a new species. The ethyl acetate extract of the E102 strain demonstrated significant efficacy at a concentration of 1000 μg/mL, resulting in the inhibition zones of 20.3–30.0 mm in size against Escherichia coli ATCC 25922; Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212. The high-performance liquid chromatography analysis identified a compound with monoisotopic mass of the substance 724.5 g/mol. Using the Chemcalc.org service, the most probable gross formulas of the required component were determined. Based on the presented calculations, there is a high probability that the substance has a sterane framework.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"914 - 922"},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Neurotransmitters on the Photosynthetic Pigment Content of the Green Microalga Haematococcus lacustris (Strains IPPAS H-239 and BM-1) 神经递质对绿微藻湖红球菌（IPPAS H-239和BM-1）光合色素含量的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825601155

Cao Boyang, T. A. Fedorenko, O. B. Chivkunova, A. E. Solovchenko, E. S. Lobakova, A. V. Oleskin

{"title":"Impact of Neurotransmitters on the Photosynthetic Pigment Content of the Green Microalga Haematococcus lacustris (Strains IPPAS H-239 and BM-1)","authors":"Cao Boyang, T. A. Fedorenko, O. B. Chivkunova, A. E. Solovchenko, E. S. Lobakova, A. V. Oleskin","doi":"10.1134/S0003683825601155","DOIUrl":"10.1134/S0003683825601155","url":null,"abstract":"The effects of the neurotransmitters serotonin (5-НТ), histamine, dopamine (DA), norepinephrine (NE), and acetylcholine (ACh) at concentrations of 0.1–10 μM on the contents of chlorophylls (а and b) and carotenoids in strains IPPAS B-239 and BM-1 of the microalga Haematococcus lacustris are considered. In the strain H. lacustris BM-1, all tested neurotransmitters except serotonin were found to stimulate carotenoid formation with an increase in the carotenoid content in the cells. The stimulatory effect was quite significant with acetylcholine and especially histamine and was manifested less with dopamine and norepinephrine. Carotenoid formation by strain IPPAS H-239 was only stimulated by acetylcholine and, to a lesser extent, by norepinephrine. The other neurotransmitters inhibited carotenoid formation. The total chlorophyll a and b content increased in the presence of all tested neurotransmitters except serotonin in strain BM-1. As for strain IPPAS H-239, its chlorophyll content was increased by acetylcholine and norepinephrine, whereas histamine and serotonin lowered the chlorophyll content. It is suggested that the tested neurotransmitters influence the vegetative cell—palmelloid cell—encysted cell transition, fixing it at the intermediate brown palmelloid stage characterized by significant chlorophyll and carotenoid contents.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"865 - 871"},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave Sensor Systems for Detection of Biologically Significant Antigens (A Review) 微波传感器系统用于检测生物重要抗原（综述）

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S000368382560071X

O. I. Guliy, B. D. Zaitsev, O. A. Karavaeva, I. A. Borodina

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Study of Localization of Endogenous N-Phenyl-2-Naphthylamine and Phthalates in Pea Seedlings (Pisum sativum L.) 豌豆幼苗内源n -苯基-2-萘胺和邻苯二甲酸盐定位的研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683824608370

L. E. Makarova, A. G. Enikeev, P. A. Bizikov, A. A. Ishchenko, I. G. Petrova, N. A. Sokolova, I. S. Kapustina

引用次数: 0

Glycation of Leghemoglobin by Methylglyoxal in Comparison with Other Hemoglobins and the Influence on Peroxidase Activity 甲基乙二醛对豆红蛋白糖化作用与其他血红蛋白的比较及对过氧化物酶活性的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825601416

E. I. Nasybullina, O. V. Kosmachevskaya, A. F. Topunov

{"title":"Glycation of Leghemoglobin by Methylglyoxal in Comparison with Other Hemoglobins and the Influence on Peroxidase Activity","authors":"E. I. Nasybullina, O. V. Kosmachevskaya, A. F. Topunov","doi":"10.1134/S0003683825601416","DOIUrl":"10.1134/S0003683825601416","url":null,"abstract":"Nonenzymatic glycation is an irreversible posttranslational protein modification, which leads to a violation of physicochemical properties and functions. Glycation most often affects lysine and arginine residues. Since hemoglobins contain many lysine residues (average 9%), they are often targets for the glycating agents glyoxal and methylglyoxal (MG). A comparative study of the susceptibility for glycation of leghemoglobin (Lb) from bean nodules (Vicia faba L.), myoglobins (Mb) from sperm whale muscles and horse hearts, and hemoglobins (Hb) from bovine and human erythrocytes was carried out. The level of glycation was defined by the autofluorescence of protein-bound advanced glycation end products (AGEs). The glycation level of Lb was 2.5 times higher than that of sperm whale Mb and human Hb and five times higher than that of horse Mb and bovine Hb. The Lb glycation level depended on the presence of oxygen in the medium. Under microaerobic conditions, the amount of AGEs formed was three times lower than in an oxygen-containing environment, and the degradation of the heme group was also slower. Glycation also affected the peroxidase activity of hemoproteins. The initial rate of Lb peroxidase reaction was six times higher than that of myoglobins and 10–13 times higher than that of hemoglobins. Glycation decreased the rate of the Lb and hemoglobin peroxidase reaction, while for myoglobins it did not change or increased depending on thte incubation time with MG.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"824 - 833"},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Transformation Conditions by Electroporation for Mycobacterium abscessus 脓肿分枝杆菌电穿孔转化条件的优化

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825601507

E. V. Zakhareva, B. A. Martini, E. G. Salina

{"title":"Optimization of Transformation Conditions by Electroporation for Mycobacterium abscessus","authors":"E. V. Zakhareva, B. A. Martini, E. G. Salina","doi":"10.1134/S0003683825601507","DOIUrl":"10.1134/S0003683825601507","url":null,"abstract":"Efficient transformation of mycobacteria, in particular, M. abscessus, is significantly complicated by the specific structure of their cell wall. The most widely used and effective method of introducing plasmid and phage DNA into mycobacterial cells is electroporation. The efficiency of electroporation is significantly affected by many factors, such as the nature of the DNA, the selective marker, growth supplements, the parameters of the electrical impulse, the species and the strain of the recipient mycobacterium. Although conditions for efficient electroporation for the slow-growing pathogen M. tuberculosis and the fast-growing saprophyte M. smegmatis have been described in details, recommendations for M. abscessus are scattered and even contradictory. Here it was established that efficient transformation of M. abscessus ATCC 19977 with the replicative vector pMV261 by electroporation is possible when using a logarithmic growth phase culture in a fairly wide range of optical density values OD600 = 0.8–4.2, while cooling has little effect on the transformation frequency. A critical parameter is the mass of the introduced DNA. It has been established that the number of transformants obtained per 1 µg of DNA increases proportionally to the square of its mass. In case of introducing less than 0.5 μg of plasmid DNA the efficiency of electroporation is insufficient.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"908 - 913"},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competitive Fluorescence Polarization Aptamer Analysis of Aflatoxin B1: Influence of the Salt Composition of the Reaction Medium on the Interaction of Reagents and the Detection Limit 黄曲霉毒素B1竞争荧光极化适体分析：反应介质盐成分对试剂相互作用及检出限的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-09-24 DOI: 10.1134/S0003683825601714

A. V. Samokhvalov, A. V. Zherdev, B. B. Dzantiev

{"title":"Competitive Fluorescence Polarization Aptamer Analysis of Aflatoxin B1: Influence of the Salt Composition of the Reaction Medium on the Interaction of Reagents and the Detection Limit","authors":"A. V. Samokhvalov, A. V. Zherdev, B. B. Dzantiev","doi":"10.1134/S0003683825601714","DOIUrl":"10.1134/S0003683825601714","url":null,"abstract":"In this study, the interaction of an aptamer having a loop structure (synthetic receptor based on single-stranded DNA) with a specific ligand aflatoxin B1 (AFB1), which is a common toxic contaminant of food products, was studied. The effect of mono- and bivalent cations (Cs+, Li+, Na+, K+, Sr2+, Ba2+, Mg2+, Ca2+) at different concentrations on the reactivity of the aptamer was studied. The fluorescence anisotropy (FA) of the fluorescein-labeled AFB1, reflecting both binding to the aptamer and the mobility of the aptamer-labeled AFB1 complex in this reaction medium, was recorded. It was shown that the FA changes differed significantly depending on the cation in the reaction medium and its concentration. The effect of monovalent cations on FA was observed in the range of 400 mM–2.5 M. For divalent cations, the influence was more pronounced in amplitude and corresponded to concentrations from 6 to 200 mM. According to the extent of FA changes, cations form the following series: Cs+ ( ll ) K+ ≤ Li+ < Na+ and Ba2+ < Sr2+ < Ca2+ < Mg2+. Comparison of the characteristics of AFB1 competitive determination for the traditionally used reaction medium and the one with a 50-fold increase in the concentration of magnesium acetate (from 20 mM to 1.0 M) showed a 12-fold decrease in the detection limit (down to 2.5 ± 0.4 nM). The obtained results allow considering the cation selection as an effective tool in the development of highly sensitive aptamer-based analytical systems.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"996 - 1002"},"PeriodicalIF":1.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, Expression and Characterisation of Newly Mined Ketoreductase in Komagataella phaffii 新发现的法菲Komagataella酮还原酶的克隆、表达及特性分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S000368382560037X

Mohd Imran Shah, K. S. C. Bose, S. Meenakshisundaram

{"title":"Cloning, Expression and Characterisation of Newly Mined Ketoreductase in Komagataella phaffii","authors":"Mohd Imran Shah, K. S. C. Bose, S. Meenakshisundaram","doi":"10.1134/S000368382560037X","DOIUrl":"10.1134/S000368382560037X","url":null,"abstract":"Optically active alcohol synthesis has been widely investigated, as they represent an important category of precursors for fine chemicals and pharmaceuticals. This study focused on the expression, purification, and characterization of the gene encoding 2,4-dienoyl-CoA reductase mined from the genome of Komagataella phaffii, here used as a ketoreductase. The impact of the promoter on the expression level of the secreted ketoreductase (kpKRED) was investigated. The kpKRED gene was inserted into an expression vector that had either an inducible alcohol oxidase 1 promoter or a constitutive glyceraldehyde-3-phosphate dehydrogenase promoter. The expressed kpKRED was purified by anion exchange chromatography and the specific activity was found to be 2902 U/mg. A catalytic efficiency Kcat/KM of 3.80 was observed for ethyl acetoacetate, with KM value of 4.68 for p-methoxy acetophenone. The purified kpKRED reduced the ketones with optimal activity at temperature 35°C and pH 6.5. Moreover, pure kpKRED exhibited broad substrate specificity towards ketones and ketoesters, with the maximum activity observed for ethyl acetoacetate. It was found that kpKRED, is capable of utilizing both NADH and NADPH as cofactors. In addition, purified kpKRED exhibits efficient solvent tolerance towards 5 organic solvents, such as ethyl alcohol and iso-propylalcohol. This enzyme retained catalytic activity in 10% organic solvents, indicating its strong resilience to organic solvents highlighting its robustness and versatility. This extensive investigation emphasizes the capability of kpKRED as a reliable and adaptable biocatalyst for industrial applications.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"844 - 855"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0