Applied Biochemistry and Microbiology最新文献

Antioxidant, UV-Protective, and Adsorption Properties of Lignins Extracted from Different Lignocellulosic Biomass Using a Deep Eutectic Solvent 用深度共熔溶剂从不同木质纤维素生物质中提取木质素的抗氧化、防紫外线和吸附性能

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826600818

M. E. Khlupova, I. S. Vasil’eva, O. V. Morozova, A. I. Yaropolov

引用次数: 0

Selective Antioxidant Response of the Pea Roots to the Effect of Compounds Mimetics of the Signals from Rhizosphere 模拟根际信号化合物对豌豆根系选择性抗氧化反应的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826600235

I. A. Tarchevsky, A. M. Egorova

引用次数: 0

Preparation, Characterization, and Study of the Properties of a Complex of Proteolytic Enzymes of Entomopathogenic Micromycete Akanthomyces cf. aculeatus 一种昆虫病原微霉菌蛋白水解酶复合物的制备、表征及性质研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683825604299

D. L. Basalaeva, A. A. Bogomolova, A. V. Koval, V. G. Kreyer, A. A. Osmolovskiy

引用次数: 0

Enzymatic Systems for the Reduction of Hemoglobins in Animals and Humans 减少动物和人类血红蛋白的酶系统

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826601149

O. E. Bagrova, O. V. Kosmachevskaya, A. F. Topunov

{"title":"Enzymatic Systems for the Reduction of Hemoglobins in Animals and Humans","authors":"O. E. Bagrova, O. V. Kosmachevskaya, A. F. Topunov","doi":"10.1134/S0003683826601149","DOIUrl":"10.1134/S0003683826601149","url":null,"abstract":"The review discusses the enzymatic reduction systems that maintain animal heme-containing globins in a physiologically active state. There are two types of reductases. Specialized systems (two-component: cytochrome-b5 reductase/cytochrome b5) provide rapid reduction of hemoglobin (Hb) and myoglobin (Mb), which is important for oxygen transport and storage. The main enzyme, CYB5R3, reduces Hb, Mb, and presumably cytoglobin (Cygb) and neuroglobin (Ngb). CYB5R4 most probably preferentially works with Cygb and Ngb in tissues with low activity of CYB5R3. Reductases with wide specificity, thio-reductase 1 (TXNRD1) and NADPH:cytochrome-P450 reductase (CPR), function as reserve systems. In addition to their role in antioxidant protection and detoxification, they effectively reduce globins (especially Cygb and Ngb), showing the relationship between cell’s redox status and globin functions. All these systems not only support oxygen transport function of globins, but also regulate nitric oxide metabolism, providing vascular tone and protection against hypoxia and oxidative stress. Their dysfunction leads to pathologies, the most severe of which is hereditary methemoglobinemia.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"619 - 636"},"PeriodicalIF":1.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Mechanism of Phthalate Biotransformation by the White-Rot Fungus Trametes hirsuta LE-BIN 072 白腐菌LE-BIN 072转化邻苯二甲酸酯的分子机制

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826600338

A. V. Shabaev, O. S. Savinova, T. V. Fedorova

{"title":"Molecular Mechanism of Phthalate Biotransformation by the White-Rot Fungus Trametes hirsuta LE-BIN 072","authors":"A. V. Shabaev, O. S. Savinova, T. V. Fedorova","doi":"10.1134/S0003683826600338","DOIUrl":"10.1134/S0003683826600338","url":null,"abstract":"Phthalic acid esters (PAEs) are ubiquitous organic xenobiotics widely distributed in the environment and pose a significant threat to human health, including concerns related to food safety. Among PAEs, di-(2-ethylhexyl) phthalate (DEHP), diisobutyl phthalate (DiBP), and butyl benzyl phthalate (BBP) are most frequently detected in the environment and agricultural products at relatively high concentrations. The presence of these PAEs in agro-industrial ecosystems can substantially affect the composition of soil and plant-associated microbial communities, leading to reduced crop yields and deterioration of agricultural product quality. To address these challenges, bioremediation approaches are commonly employed, including microbial degradation and mycoremediation. In the present study, the intracellular degradation mechanisms of DEHP, DiBP, and BBP by the white-rot fungus Trametes hirsuta LE-BIN 072 were investigated using a proteomic approach. Among the enzymes involved in the degradation of these phthalates, various esterases, alcohol dehydrogenases (ADHs), aldehyde dehydrogenases (ALDHs), aldo-keto reductases of families 7 and 13 (AKR7A and AKR13A), short-chain dehydrogenases/reductases (SDRs), and several other oxidoreductases were identified.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"721 - 732"},"PeriodicalIF":1.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683826600338.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extremophilic and Extremotolerant Nitrile-Hydrolyzing Microorganisms: Distribution and Biotechnological Potential (Review) 嗜极和耐极腈水解微生物：分布和生物技术潜力（综述）

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683825603828

G. A. Syrovatskaya, A. Yu. Maksimov, Yu. G. Maksimova

{"title":"Extremophilic and Extremotolerant Nitrile-Hydrolyzing Microorganisms: Distribution and Biotechnological Potential (Review)","authors":"G. A. Syrovatskaya, A. Yu. Maksimov, Yu. G. Maksimova","doi":"10.1134/S0003683825603828","DOIUrl":"10.1134/S0003683825603828","url":null,"abstract":"The ubiquity of nitriles today and, according to one hypothesis, their presence as one of the basic substrates during the emergence of life in the early stages of Earth’s history, has led to the emergence of microorganisms producing nitrile-hydrolyzing enzymes among a wide variety of phylogenetic and ecological groups, including extremophilic prokaryotes and a small number of unicellular eukaryotic organisms. This review summarizes information on nitrile-degrading microorganisms isolated from extreme biotopes that differ in origin and type of aggressive environmental conditions. Nitrile-hydrolyzing enzymes, their substrate preferences, whole-cell culture conditions, and optimal environmental parameters for nitrile-hydrolyzing activity are characterized. The main areas of study and application of nitrile-utilizing microorganisms are biotechnological (use as biocatalysts for various chemical processes and the biodegradation of toxic cyanides and nitriles), ecological (clarification of the role of nitriles in the nitrogen cycle), and evolutionary (molecular clock dating). Particular attention is given to the role of nitriles in the natural nitrogen cycle.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"637 - 652"},"PeriodicalIF":1.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Resazurin for Studying the Intracellular Activity of Low Molecular Weight Anti-Tuberculosis Compounds 利用瑞沙脲研究低分子量抗结核化合物的胞内活性

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826601216

K. A. Dementieva, B. A. Martini, K. B. Majorov, E. G. Salina

引用次数: 0

Evaluation of the Effect of Ampicillin on Bacterial Biofilms Using a Compact Analyzer Based on a Lateral Electric Field Resonator 用基于侧电场谐振器的紧凑型分析仪评价氨苄西林对细菌生物膜的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826600570

O. I. Guliy, B. D. Zaitsev, O. A. Karavaeva, S. S. Evstigneeva, A. A. Shirokov, A. A. Teplykh, I. A. Borodina

引用次数: 0

Potential of Silver Nanoparticles Produced Using Green Synthesis Technology as Antibacterial Drugs 绿色合成技术制备纳米银抗菌药物的潜力

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-08-21 DOI: 10.1134/S0003683826600582

O. I. Guliy, O. A. Karavaeva, L. A. Dykman

{"title":"Potential of Silver Nanoparticles Produced Using Green Synthesis Technology as Antibacterial Drugs","authors":"O. I. Guliy, O. A. Karavaeva, L. A. Dykman","doi":"10.1134/S0003683826600582","DOIUrl":"10.1134/S0003683826600582","url":null,"abstract":"The emergence of drug resistance in microbial cells is a serious public health problem. Therefore, the development of new alternative antibacterial agents to combat multidrug-resistant bacteria is becoming increasingly important. The rapid development of nanotechnology and nanoparticle synthesis has facilitated the emergence of new strategies for combating microbial cells. Silver nanoparticles, which possess antibacterial activity, are particularly valuable. Nanoparticle synthesis involves various physical and chemical methods using toxic reagents that pollute the environment. Therefore, cost-effective alternative processes for synthesizing nanomaterials using biological systems, biological or “green” nanoparticle synthesis, are being actively developed. Natural biological sources, including fungi, bacteria, plants, animal cells, viruses, and their metabolites, can be used for green nanoparticle synthesis. The use of biological objects to create inorganic nanoparticles is a relatively new area of biotechnology. Green synthesis of noble metal nanoparticles is a vast and growing area of research. This paper presents the basic principles of green synthesis technology for producing silver nanoparticles, as well as the advantages and disadvantages of its application. The potential for using nanoparticles obtained using green synthesis technology as antimicrobial agents is discussed.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"653 - 669"},"PeriodicalIF":1.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Characterization of a Thermostable Broad-pH Antimicrobial Protein from Bacillus subtilis FB123 枯草芽孢杆菌FB123热稳定型宽ph抗菌蛋白的生化特性

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-09 DOI: 10.1134/S0003683825604822

X. Luo, Y. Lin, B. Shi

{"title":"Biochemical Characterization of a Thermostable Broad-pH Antimicrobial Protein from Bacillus subtilis FB123","authors":"X. Luo, Y. Lin, B. Shi","doi":"10.1134/S0003683825604822","DOIUrl":"10.1134/S0003683825604822","url":null,"abstract":"To mitigate the increase in Staphylococcus aureus resistance resulting from antibiotic misuse, this study aimed to identify natural and safe antibacterial substances as alternatives to conventional antibiotics for treating S. aureus infections in animal husbandry. An antibacterial protein (APBS) with broad pH and thermal stability was successfully purified from the culture supernatant of Bacillus subtilis FB123 using ammonium sulfate precipitation, Sephacryl S-200 High Resolution gel filtration chromatography, and DEAE Sepharose Fast Flow ion exchange chromatography. APBS exhibited a significant inhibitory effect against S. aureus, producing an inhibition zone diameter up to 16.5 mm. Investigation of its physicochemical properties revealed that APBS displayed optimal antibacterial activity at 30°C and pH 6.0, and retained high activity even after treatment at 121°C and across a broad pH range from 2.0 to 9.0. The molecular weight of APBS was estimated to be approximately 14.8 kDa by SDS-PAGE, and its isoelectric point was determined to be 3.64. Furthermore, this protein demonstrated broad spectrum of antibacterial activity against other Gram-positive bacteria and fungi. Finally, the effects of various proteases, metal ions, and organic solvents on its activity were investigated to assess the potential practical application of APBS. This study indicates that the APBS from B. subtilis FB123 holds promising potential for development as a novel anti-S. aureus agent.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"698 - 706"},"PeriodicalIF":1.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148781971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0