Applied Biochemistry and Microbiology最新文献

Identification of Mitogen-activated Protein Kinase from Schizochytrium sp. and Application in Resisting Stress Environments Schizochytrium sp.丝裂原活化蛋白激酶的鉴定及其在抗逆性环境中的应用

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S000368382304004X

C. Du, J. Jiang, X. Hu, L. Ren

{"title":"Identification of Mitogen-activated Protein Kinase from Schizochytrium sp. and Application in Resisting Stress Environments","authors":"C. Du, J. Jiang, X. Hu, L. Ren","doi":"10.1134/S000368382304004X","DOIUrl":"10.1134/S000368382304004X","url":null,"abstract":"Schizochytrium sp. is a marine fungus, which usually lives in the salinity of 40–100% seawater and has unique salt tolerance characteristics. In this study, we explored the candidate mitogen-activated protein kinase (MAPK) related to the salt-tolerant regulatory network from Schizochytrium sp. Firstly, 6 potential MAPK genes from Schizochytrium sp. were identified based on the genome information and bioinformatics analysis. Then, the MAPK gene expression of Schizochytrium sp. under different cultivation conditions locked two genes encoding Mpk6315 and Mpk2022. Furthermore, overexpressing of Mpk6315 in S. cerevisiae enhanced cell growth by 62% while and Mpk2022 overexpression improved the membrane integrity under different stress conditions, indicating that Mpk6315 might play an important role for cell growth and Mpk2022 could help cells to encounter different environmental stressors. This study was the first time to identify the MAPK genes from Schizochytrium sp., which could enlarge the MAPK modules of salt stress tolerance and provide new elements for improving the stress resistance of other microorganisms.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"438 - 449"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4787561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melanin Production by the Medicinal Mushroom Inonotus obliquus F-1375 in Submerged Liquid Cultivation and its Antiretroviral Properties 药用菌Inonotus obliquus F-1375液体培养的黑色素生成及其抗逆转录病毒特性

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040142

T. V. Teplyakova, N. A. Markovich, N. M. Gashnikova, M. P. Gashnikova

{"title":"Melanin Production by the Medicinal Mushroom Inonotus obliquus F-1375 in Submerged Liquid Cultivation and its Antiretroviral Properties","authors":"T. V. Teplyakova, N. A. Markovich, N. M. Gashnikova, M. P. Gashnikova","doi":"10.1134/S0003683823040142","DOIUrl":"10.1134/S0003683823040142","url":null,"abstract":"<div>We have explored the effect of a new strain of the mushroom Inonotus obliquus (Ach.:Pers.) Pilat (Basidiomycota) F-1375 on melanin production in a glucose–tryptone medium, while adding a preparation of betulin and chemically pure tyrosine, under electric lighting and under dark conditions. The betulin preparation in the examined concentrations had practically no effect on the production of melanin, while high concentrations of tyrosine stimulated the production of melanin, both under illumination and in the dark. All studied samples of mushroom melanin are nontoxic: both isolated from natural (TC50 = 96 ± 8 μg/mL) and cultured in a liquid medium with the addition of betulin and tyrosine (TC50 from 164 to 400 μg/ml) are active against HIV-1 in an MT-4 cell culture (IC50 1 to 4.3 μg/mL, SI 17 to 192). The selectivity indices of the preparations of melanin of cultivated Inonotus obliquus F-1375 against the human immunodeficiency virus subtype A were at the level of 33-192, which makes it possible to recommend them for the development of antiviral agents. Melanin from the mushroom Inonotus obliquus F-1375 cultured in electric light with the addition of 10 and 20 mM tyrosine had the highest selectivity index for the HIV-1 retrovirus (SI 192).</div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"482 - 488"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4486200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Growth-promoting Bacterial Consortia on Overall Growth of Tomato Plants 促生菌群对番茄植株整体生长的影响

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040105

V. Pattani, J. Kaneriya, K. Joshi, D. Gandhi, G. Sanghvi

{"title":"Effect of Growth-promoting Bacterial Consortia on Overall Growth of Tomato Plants","authors":"V. Pattani, J. Kaneriya, K. Joshi, D. Gandhi, G. Sanghvi","doi":"10.1134/S0003683823040105","DOIUrl":"10.1134/S0003683823040105","url":null,"abstract":"The present work was focused on plant growth-promoting bacteria (PGPB) for the enhanced production of tomatoes. The effect of 14 indigenous PGPB isolated from the soil samples collected from Gir National forest areas (Dist. Junagadh, Gujarat, India) and the coastal region of Saurashtra (Dist. Gir-Somnath, Gujarat, India) on tomato seedlings was studied. Only 6 isolates showed positive results in in vitro biochemical and plant-growth-promoting assays such as phosphate and zinc solubilization, siderophores and HCN production and antibacterial and antifungal properties. The rest isolates were negative to one or more of the selected tests. Based on in vitro results, all PGPB were tested on tomato seedlings. Four stage screenings were used to select the best combinations. After the primary screening, 9 isolates were selected for further experiments based on the germination, height of plants and seedling vigor. Plant height, leaf count, seedling vigor and total chlorophyll were measured. Leaf anatomy was studied at the end of the quaternary trial to understand the changes at a cellular level and it revealed the anatomical changes such as increased chlorophyll at cellular level and enhanced starch production. Microbial consortia showed better results compated to single-inoculant treatment. Out of 503 different combinations tested at secondary trial, 129 were selected for tertiary trials and 24 from them were further qualified for quaternary trials. At the end of quaternary trials, total 04 combinations were selected for future experiments on field. The quaternary trial strategy helped to reduce the total 503 possible combinations to 04 combinations improving germination, height, seedling vigor, number of leaves and chlorophyll content compared to non-treated tomato plants. Further trials were carried out in field for long period of time to measure the profound effect of selected consortium in real life.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"511 - 521"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4484993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Efficiency of Various DNA Polymerases for Amplification of Long Sequences from Genomic DNA and cDNA of Cultivated Potatoes 不同DNA聚合酶对栽培马铃薯基因组DNA和cDNA长序列扩增的效率

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040026

A. D. Antipov, N. E. Zlobin

{"title":"The Efficiency of Various DNA Polymerases for Amplification of Long Sequences from Genomic DNA and cDNA of Cultivated Potatoes","authors":"A. D. Antipov, N. E. Zlobin","doi":"10.1134/S0003683823040026","DOIUrl":"10.1134/S0003683823040026","url":null,"abstract":"Amplification of long fragments from complex templates, such as eukaryotic genomic DNA, is considered a difficult task for most DNA polymerases. In this research, six DNA polymerases were used to amplify full-length sequences from the genomic DNA of Solanum tuberosum genes encoding translation initiation factors of the eIF4E family, as well as for the synthesis of fragments of the potato Y virus genome from cDNA of potato plants infected by this virus. It was found that the efficiency of amplification by various DNA polymerases generally decreased with the increasing length of the amplicons. LongAmp and Platinum SuperFi II polymerases demonstrated the highest efficiency in the synthesis of long fragments, which made it possible to synthesize PCR products with a length of more than 10 000 base pairs with high efficiency. The lowest efficiency was demonstrated by Encyclo polymerase. None of the DNA polymerases provided efficient amplification of all the studied DNA fragments. At the same time, any of the studied DNA fragments could be effectively amplified using at least one DNA polymerase variant. Thus, the choice of DNA polymerase was of key importance for the efficiency of the synthesis of a desired PCR product.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"522 - 529"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683823040026.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4487980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in Emericellopsos alkalina under Different Cultivation Conditions 不同培养条件下碱绿藻中抗菌肽绿藻素异构体的形成

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823320017

A. E. Kuvarina, M. A. Sukonnikov, E. A. Rogozhin, M. V. Serebryakova, A. V. Timofeeva, M. L. Georgieva, V. S. Sadykova

引用次数: 0

Optimization of a Purification Method for the Recombinant Platelet-Derived Growth Factor rhPDGF-BB Expressed in the Methylotrophic Yeast Pichia Pastoris 甲基营养酵母毕赤酵母中表达重组血小板衍生生长因子rhPDGF-BB的纯化方法优化

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040087

A.-A. V. Misterova, V. A. Chicherin, A. S. Gerasimov

引用次数: 0

The Perspective Properties and Directions of Bacillus thuringiensis Use for Plant Protection 苏云金芽孢杆菌在植物保护中的应用前景、性质及发展方向

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040075

R. M. Khairullin, A. V. Sorokan, V. F. Gabdrakhmanova, I. V. Maksimov

引用次数: 0

The Corrosive Activity of Microorganisms Isolated from Fouling of Structural Materials in the Coastal Zone of the Barents Sea 巴伦支海海岸带结构材料污垢中分离微生物的腐蚀活性

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040166

D. Yu. Vlasov, A. L. Bryukhanov, G. G. Nyanikova, M. S. Zelenskaya, I. M. Tsarovtseva, A. R. Izatulina

{"title":"The Corrosive Activity of Microorganisms Isolated from Fouling of Structural Materials in the Coastal Zone of the Barents Sea","authors":"D. Yu. Vlasov, A. L. Bryukhanov, G. G. Nyanikova, M. S. Zelenskaya, I. M. Tsarovtseva, A. R. Izatulina","doi":"10.1134/S0003683823040166","DOIUrl":"10.1134/S0003683823040166","url":null,"abstract":"<div>Potentially corrosion-active microorganisms isolated from structural materials with the signs of biofouling on the coast of Kislaya Bay (Barents Sea, Russia) were studied: sulfate-reducing, iron-oxidizing and sulfur-oxidizing bacteria. The cultures of sulfate-reducing bacteria (Desulfovibrio sp., Halodesulfovibrio sp.), sulfur-oxidizing bacteria (Dietzia sp.), and iron-oxidizing bacteria (Pseudomonas fluorescens, Bacillus sp.) were identified on the basis of determining the nucleotide sequences of the 16S rRNA gene. The methods of scanning electron microscopy, energy dispersive microanalysis of the chemical composition, and powder X-ray diffraction analysis revealed significant changes in the structure and chemical composition of the surface layer of steel reinforcement samples exposed for 28 days in the presence of isolated microorganisms that demonstrated their active participation in corrosion processes. It has been shown that the formation of mineral analogues in corrosion products depends on the strains of studied bacteria and the peculiarities of their metabolism. Sulfate-reducing bacteria isolated from the littoral zone of the Barents Sea showed the highest activity in the development of corrosion processes.</div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"425 - 437"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4486197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp. 两种不动杆菌吲哚降解代谢物的代谢分析。

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040154

T. A. Tesso, G. Liu

{"title":"Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.","authors":"T. A. Tesso, G. Liu","doi":"10.1134/S0003683823040154","DOIUrl":"10.1134/S0003683823040154","url":null,"abstract":"Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human, animals and ecological environment. Bacteria play vital role in the degradation and biotransformation of such persistent organic and harmful pollutants. In this experiment, Acinetobacter towneri NTA1-2A and Acinetobacter guillouiae TAT1-6A were used to degrade indole. Indole-degradated metabolites were analyzed by metabolomics analysis of fermentation broth of these strains using LC-MS/MS. The metabolomics analysis showed indole-5-carbonitrile 3-benzoyl (C16H10N2O), dimethyl sulfoxide (C2H6OS), deoxyguanosine (C10H13N5O4), Leu (C6H13NO2), and N-nitroso-pyrrolidine were indole-degradated metabolites formed by A. towneri NTA1-2A while neurine (C5H13NO) and norvaline (C5H11NO2) are common metabolites of both strains. The two strains' degradation metabolites differed from those reported in other studies, demonstrating the diversity of the distinct microbial strains approach to the metabolism of indolic compounds. Extracellular enzymes released to culture medium by these bacteria were also identified. In conclusion, the two bacterial strains have a potential of converting notorious indole compound to non-toxic intermediates mediated by enzymes. Hence, future study should focus on biochemical pathway and genetic basis for indole-degradated metabolites in the two strains.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"462 - 467"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4486208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of 36-kDa Dimeric Methotrexate-degrading Enzyme from Variovorax paradoxus, its Performance and Stability of its Cross-linked Aggregates 36-kDa二聚甲氨蝶呤降解酶的表征、性能及其交联聚集体的稳定性

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S0003683823040129

C. K. Sahu, V. K. Bayineni, J. S. Hampapura, C. M. Hussain, R.-K. Kadeppagari

{"title":"Characterization of 36-kDa Dimeric Methotrexate-degrading Enzyme from Variovorax paradoxus, its Performance and Stability of its Cross-linked Aggregates","authors":"C. K. Sahu, V. K. Bayineni, J. S. Hampapura, C. M. Hussain, R.-K. Kadeppagari","doi":"10.1134/S0003683823040129","DOIUrl":"10.1134/S0003683823040129","url":null,"abstract":"In the present study, methotrexate (MTX)-degrading 36-kDa dimer was characterized from the 0–80% ammonium sulphate precipitate of Variovorax paradoxus cell lysate. This enzyme showed optimum activity at 35°C and pH 6.5 with KM value of 199.6 µM for MTX which are different from those of 46-kDa dimer. Micro- or nano-sized cross-linked aggregates of new enzyme showed better stability in the presence of serum than the native soluble form. Addition of an endogenic carrier protein, human serum albumin (HSA) to the enzyme aggregates further improved their serum stability. Nanoaggregates showed better serum stability over microaggregates. Nanoaggregates of the enzyme degraded the MTX faster than soluble form and microaggregates due to their lower KM values on MTX, whereas microaggregates were slower than soluble enzyme itself due to their higher KM values. Hence, cross-linked aggregates of MTX- degrading enzyme isolated from V. paradoxus shown better performance in their nanoform compared to the microform. Nanoaggregates of enzyme revealed the highest functionality and serum stability that makes them more suitable for therapeutic applications.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"468 - 475"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4487973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0