L. E. Makarova, A. G. Enikeev, P. A. Bizikov, A. A. Ishchenko, I. G. Petrova, N. A. Sokolova, I. S. Kapustina
{"title":"豌豆幼苗内源n -苯基-2-萘胺和邻苯二甲酸盐定位的研究","authors":"L. E. Makarova, A. G. Enikeev, P. A. Bizikov, A. A. Ishchenko, I. G. Petrova, N. A. Sokolova, I. S. Kapustina","doi":"10.1134/S0003683824608370","DOIUrl":null,"url":null,"abstract":"<p>The content of negative allelopathic substances, N-phenyl-2-naphthylamine (N-PNA) and phthalates, was studied in morphologically and physiologically different root sites, in the epicotile of etiolated pea seedlings (<i>Pisum sativum</i> L.) and in the callus obtained from their epicotiles. The detection of N-PNA, <i>o</i>-phthalic acid, and phthalates in callus cells is evidence of the independent biosynthesis of these compounds in pea plant cells. Based on the changes in the content of N-PNA and phthalates caused by 10<sup>–4</sup> M naphthalene, it was shown that the main loci of biosynthesis of these compounds in pea seedlings include the growing apical part of the root and the epicotyle. It has been suggested that legumes, characterized by the ability to metabolize these substances with the participation of endophytic bacteria capable of degrading PAHs, can be used for remediation of soils contaminated with PAH pollutants.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"967 - 972"},"PeriodicalIF":1.1000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study of Localization of Endogenous N-Phenyl-2-Naphthylamine and Phthalates in Pea Seedlings (Pisum sativum L.)\",\"authors\":\"L. E. Makarova, A. G. Enikeev, P. A. Bizikov, A. A. Ishchenko, I. G. Petrova, N. A. Sokolova, I. S. Kapustina\",\"doi\":\"10.1134/S0003683824608370\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The content of negative allelopathic substances, N-phenyl-2-naphthylamine (N-PNA) and phthalates, was studied in morphologically and physiologically different root sites, in the epicotile of etiolated pea seedlings (<i>Pisum sativum</i> L.) and in the callus obtained from their epicotiles. The detection of N-PNA, <i>o</i>-phthalic acid, and phthalates in callus cells is evidence of the independent biosynthesis of these compounds in pea plant cells. Based on the changes in the content of N-PNA and phthalates caused by 10<sup>–4</sup> M naphthalene, it was shown that the main loci of biosynthesis of these compounds in pea seedlings include the growing apical part of the root and the epicotyle. It has been suggested that legumes, characterized by the ability to metabolize these substances with the participation of endophytic bacteria capable of degrading PAHs, can be used for remediation of soils contaminated with PAH pollutants.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"61 5\",\"pages\":\"967 - 972\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683824608370\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824608370","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Study of Localization of Endogenous N-Phenyl-2-Naphthylamine and Phthalates in Pea Seedlings (Pisum sativum L.)
The content of negative allelopathic substances, N-phenyl-2-naphthylamine (N-PNA) and phthalates, was studied in morphologically and physiologically different root sites, in the epicotile of etiolated pea seedlings (Pisum sativum L.) and in the callus obtained from their epicotiles. The detection of N-PNA, o-phthalic acid, and phthalates in callus cells is evidence of the independent biosynthesis of these compounds in pea plant cells. Based on the changes in the content of N-PNA and phthalates caused by 10–4 M naphthalene, it was shown that the main loci of biosynthesis of these compounds in pea seedlings include the growing apical part of the root and the epicotyle. It has been suggested that legumes, characterized by the ability to metabolize these substances with the participation of endophytic bacteria capable of degrading PAHs, can be used for remediation of soils contaminated with PAH pollutants.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
