K. V. Kiselev, A. S. Dubrovina, Z. V. Ogneva, O. A. Aleynova
{"title":"低温刺激葡萄CPK26基因的选择性剪接","authors":"K. V. Kiselev, A. S. Dubrovina, Z. V. Ogneva, O. A. Aleynova","doi":"10.1134/S0003683825600873","DOIUrl":null,"url":null,"abstract":"<p>Alternative splicing (AS) is a non-canonical gene splicing process that allows a single gene to synthesise multiple protein isoforms and enhance a variety of protein functions. In this study, the involvement of AS in the generation of plant resistance to abiotic stresses was investigated using the <i>VaCPK26</i> calcium-dependent protein kinase (CPK) gene, which is responsible for the resistance of <i>Vitis amurensis</i> Rupr. grapes to soil salinity and drought. The level of <i>VaCPK26</i> transcription in grape leaves was studied under the influence of different environmental factors. Under low temperature exposure, in addition to the full-length <i>VaCPK26</i> transcript, a short-spliced <i>VaCPK26s1</i> transcript was obtained that lacked the 2nd exon out of the 7 exons that make up the full-length <i>VaCPK26</i>. Recombinant <i>VaCPK26</i> increased the resistance of grape cells to salt stress and drought, and overexpression of the spliced <i>VaCPK26s1</i> transcript in <i>V. amurensis</i> grape cell cultures had no effect on resistance to the stresses tested. These results show that AS can lead to the loss of properties of spliced transcripts characteristic of the original full-length form, which is important for complete understanding of the biological functions of CPK and alternative splicing.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"987 - 995"},"PeriodicalIF":1.1000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Low Temperatures Stimulate Alternative Splicing of the CPK26 Gene in Vitis amurensis Grapes\",\"authors\":\"K. V. Kiselev, A. S. Dubrovina, Z. V. Ogneva, O. A. Aleynova\",\"doi\":\"10.1134/S0003683825600873\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Alternative splicing (AS) is a non-canonical gene splicing process that allows a single gene to synthesise multiple protein isoforms and enhance a variety of protein functions. In this study, the involvement of AS in the generation of plant resistance to abiotic stresses was investigated using the <i>VaCPK26</i> calcium-dependent protein kinase (CPK) gene, which is responsible for the resistance of <i>Vitis amurensis</i> Rupr. grapes to soil salinity and drought. The level of <i>VaCPK26</i> transcription in grape leaves was studied under the influence of different environmental factors. Under low temperature exposure, in addition to the full-length <i>VaCPK26</i> transcript, a short-spliced <i>VaCPK26s1</i> transcript was obtained that lacked the 2nd exon out of the 7 exons that make up the full-length <i>VaCPK26</i>. Recombinant <i>VaCPK26</i> increased the resistance of grape cells to salt stress and drought, and overexpression of the spliced <i>VaCPK26s1</i> transcript in <i>V. amurensis</i> grape cell cultures had no effect on resistance to the stresses tested. These results show that AS can lead to the loss of properties of spliced transcripts characteristic of the original full-length form, which is important for complete understanding of the biological functions of CPK and alternative splicing.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"61 5\",\"pages\":\"987 - 995\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683825600873\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683825600873","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Low Temperatures Stimulate Alternative Splicing of the CPK26 Gene in Vitis amurensis Grapes
Alternative splicing (AS) is a non-canonical gene splicing process that allows a single gene to synthesise multiple protein isoforms and enhance a variety of protein functions. In this study, the involvement of AS in the generation of plant resistance to abiotic stresses was investigated using the VaCPK26 calcium-dependent protein kinase (CPK) gene, which is responsible for the resistance of Vitis amurensis Rupr. grapes to soil salinity and drought. The level of VaCPK26 transcription in grape leaves was studied under the influence of different environmental factors. Under low temperature exposure, in addition to the full-length VaCPK26 transcript, a short-spliced VaCPK26s1 transcript was obtained that lacked the 2nd exon out of the 7 exons that make up the full-length VaCPK26. Recombinant VaCPK26 increased the resistance of grape cells to salt stress and drought, and overexpression of the spliced VaCPK26s1 transcript in V. amurensis grape cell cultures had no effect on resistance to the stresses tested. These results show that AS can lead to the loss of properties of spliced transcripts characteristic of the original full-length form, which is important for complete understanding of the biological functions of CPK and alternative splicing.
期刊介绍:
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.