Applied Biochemistry and Microbiology最新文献_第10页

Fungal Hydrophobins: Biosynthesis, Properties, and Possibilities of Application in Biotechnology (Review) 真菌疏水性蛋白：生物合成、特性及在生物技术中的应用前景（综述）

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603603

E. V. Lopatukhin, Yu. A. Ihalainen, N. N. Markelova, A. E. Kuvarina, V. S. Sadykova

引用次数: 0

Obtaining Analogues of Fermented Milk Products from Seed Meal Using New Strains of Lactic Acid Bacteria 利用乳酸菌新菌株从种子粉中获得类似的发酵乳制品

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603664

A. V. Sinelnikov, T. V. Kolganova, R. V. Ulanova

{"title":"Obtaining Analogues of Fermented Milk Products from Seed Meal Using New Strains of Lactic Acid Bacteria","authors":"A. V. Sinelnikov, T. V. Kolganova, R. V. Ulanova","doi":"10.1134/S0003683824603664","DOIUrl":"10.1134/S0003683824603664","url":null,"abstract":"A method has been developed for producing analogs of fermented milk drinks from pumpkin seed meal, which is a massive waste product from oilseed production, using new strains of lactic acid bacteria (LAB) isolated from different samples of kumiss. Based on the results of screening 50 LAB isolates capable of fermenting milk and aqueous meal extracts in a wide pH range, three strains with the best growth characteristics were selected. These strains were identified as representatives of the genus Lacticaseibacillus, most closely related to L. rhamnosus and L. casei (with 99.93 and 99.65% similarity in 16S rRNA gene sequences). An optimal scheme for producing drinks has been selected, including grinding meal, optimized extraction with alkaline solutions, heat treatment of the extract to remove foreign microflora, introduction of the inoculum (3–5% vol/vol) of new LAB strains, and fermentation at 37oС for ten hours. Compared with the fermented milk product obtained by fermenting milk with the same microorganisms, the drink made from meal extracts was distinguished by the absence of lactose and cholesterol, and increased content of unsaturated fatty acids (2.3 times) and protein (1.7 times), and the presence of essential amino acids in proteins. Thus, pumpkin seed meal, which is still used ineffectively, is a good basis for obtaining analogs of fermented milk products with beneficial properties. The developed method for producing lacto-fermented drinks can be adapted for processing other types of meals and cakes.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"476 - 482"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Diatom Nanofrustulum shiloi As a Promising Species in Modern Biotechnology 硅藻 Nanofrustulum shiloi--现代生物技术中的潜力物种

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603615

A. A. Blaginina, S. N. Zheleznova, E. S. Miroshnichenko, R. G. Gevorgiz, L. I. Ryabushko

{"title":"The Diatom Nanofrustulum shiloi As a Promising Species in Modern Biotechnology","authors":"A. A. Blaginina, S. N. Zheleznova, E. S. Miroshnichenko, R. G. Gevorgiz, L. I. Ryabushko","doi":"10.1134/S0003683824603615","DOIUrl":"10.1134/S0003683824603615","url":null,"abstract":"The results of studies of an intensive culture of a new species of bentoplanktonic diatom N. shiloi (Lee, Reimer et McEnery) Round, Hallsteinsen et Paasche 1999 isolated from the Black Sea are presented. Detailed descriptions are provided of the methods used to isolate the species into an algologically pure culture and its morphological and taxonomic features under light and electron scanning microscopy. The biochemical and production characteristics were also studied including the ability of the strain to accumulate fucoxanthin (Fx) and polyunsaturated fatty acids (PUFA) in laboratory conditions. During the exponential growth phase, the specific culture growth rate was µ = 0.8 1/day, and the maximum productivity P = 0.46 g dry weight/(L day). The accumulation of PUFAs in the biomass of N. shiloi reached 67.39 mg/g dry weight of algae. The Fx concentration in the biomass at the beginning of the stationary growth phase was 10 mg/g dry weight. The fairly high rate of Fx biosynthesis in microalgae cells, as well as the composition of fatty acids of the Black Sea strain, makes it possible to classify N. shiloi as a promising object in biotechnology.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"483 - 495"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140968557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decolorization of Crystal Violet by a Mixed Culture under the Influence of Bioelectrochemical Stimulation 生物电化学刺激下混合培养物对水晶紫的脱色作用

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603585

A. A. Samkov, E. V. Pankratova, M. N. Kruglova, A. V. Bespalov, S. M. Samkova, N. N. Volchenko, A. A. Khudokormov

{"title":"Decolorization of Crystal Violet by a Mixed Culture under the Influence of Bioelectrochemical Stimulation","authors":"A. A. Samkov, E. V. Pankratova, M. N. Kruglova, A. V. Bespalov, S. M. Samkova, N. N. Volchenko, A. A. Khudokormov","doi":"10.1134/S0003683824603585","DOIUrl":"10.1134/S0003683824603585","url":null,"abstract":"A significant variation in the relative representation of copies of bacterial genes of dye-decolorizing DyP peroxidases typical for the genus Shewanella and a number of other microorganisms was found in the bottom sediments of freshwater reservoirs. It was found that the specific rate of decolorization of crystal violet in a laboratory bioelectrochemical system by a mixed culture of bottom sediments, which showed the highest representation of DyP genes, depended on the method of electrical stimulation of the external circuit and the concentration of the dye. After an increase in the concentration of more than 20 microns, the maximum speed was achieved in the presence of an ionistor polarly connected to the external electrical circuit of the bioelectrochemical system and amounted to 3.23 ± 0.11 μM/h, while with the opposite polarity connection, a minimum value of 2.07 ± 0.08 μM/h was observed. In the case of an open circuit and a resistor, similar indicators occurred with 2.88 ± 0.09 and 2.67 ± 0.12 μM/h, respectively. When analyzing the decolorization products, a consistent decrease in the maxima of the absorption bands of the dye was noted, indicating its more complete degradation by the mixed culture. The results may be of interest for the development of methods to improve the efficiency of bioelectrochemical methods of environmental biotechnology by electrostimulation of the external circuit.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"467 - 475"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Chymotrypsin-like Peptidase from Tenebrio molitor with a Non-Canonical Substrate-Binding Site 具有非典型底物结合位点的重组糜蛋白酶样肽酶

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603652

V. F. Tereshchenkova, N. I. Zhiganov, A. S. Gubaeva, F. I. Akentyev, Ya. E. Dunaevsky, D. G. Kozlov, M. A. Belozersky, E. N. Elpidina

{"title":"Recombinant Chymotrypsin-like Peptidase from Tenebrio molitor with a Non-Canonical Substrate-Binding Site","authors":"V. F. Tereshchenkova, N. I. Zhiganov, A. S. Gubaeva, F. I. Akentyev, Ya. E. Dunaevsky, D. G. Kozlov, M. A. Belozersky, E. N. Elpidina","doi":"10.1134/S0003683824603652","DOIUrl":"10.1134/S0003683824603652","url":null,"abstract":"We characterized an alkaline chymotrypsin-like serine peptidase from the yellow mealworm Te-nebrio molitor with a non-canonical substrate-binding subsite for its possible application as a component (an additive) in various biological products. The enzyme was obtained as a recombinant preparation. Purification was carried out using affinity chromatography on Ni2+-NTA agarose. The specificity constants (kcat/KM) for the chymotrypsin substrates, Glp-AAF-pNA, Suc-AAPF-pNA, and Ac-Y-pNA, were 7, 4.2, and 0.9 (µM min)–1, respectively. The optimum of the proteolytic activity was observed at pH 9.0. The enzyme was stable in the alkaline pH range, and in the presence of BSA, also in the acidic region. Peptidase was inhi-bited by synthetic inhibitors such as PMSF, TPCK, and chymostatin, while EDTA, E-64, and pepstatin had no effect on the enzyme activity. The purified enzyme showed high stability over time in the presence of BSA. The short life cycle of the insect and the production of a large number of peptidases in the midgut with high catalytic activity and stability can make T. molitor an excellent alternative source of industrially important enzymes for application as components (additives) in various biological products (e.g., stain removers, detergents, etc.).","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"420 - 430"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of an Antibacterial Composite Aerogel for Biomedical Purposes Based on an Alginate–Chitosan Complex and Calcium Carbonate 基于海藻酸盐-壳聚糖复合物和碳酸钙制备用于生物医学目的的抗菌复合气凝胶

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI: 10.1134/S0003683824020042

N. A. Gorshkova, O. S. Brovko, I. A. Palamarchuk, A. D. Ivahnov, N. I. Bogdanovich, T. Ya. Vorob’eva

引用次数: 0

Isolation of Chitosan-Melanin Complex from Black Soldier Fly Adults and Obtaining Nanofibrous Materials Based on It 从黑皮蝇成虫中分离壳聚糖-黑色素复合物并获得基于它的纳米纤维材料

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI: 10.1134/S0003683824020078

A. Sh. Khayrova, N. A. Sazhnev, D. V. Korobovskaya, S. A. Lopatin, N. R. Kildeeva, V. P. Varlamov

引用次数: 0

The α-Amylase and α-Glucosidase Inhibitiory Effects and Antioxidant Properties of Native Lactobacilli Strains Isolated from Traditional Fermented Dairy Products 从传统发酵乳制品中分离的本地乳酸菌株的α-淀粉酶和α-葡萄糖苷酶抑制作用及抗氧化特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI: 10.1134/S0003683824020170

F. Shirkhan, S. Mirdamadi, M. Mirzaei, B. Akbari-adergani, N. Nasoohi

{"title":"The α-Amylase and α-Glucosidase Inhibitiory Effects and Antioxidant Properties of Native Lactobacilli Strains Isolated from Traditional Fermented Dairy Products","authors":"F. Shirkhan, S. Mirdamadi, M. Mirzaei, B. Akbari-adergani, N. Nasoohi","doi":"10.1134/S0003683824020170","DOIUrl":"10.1134/S0003683824020170","url":null,"abstract":"Diabetes is the third leading cause of premature death worldwide due to hyperglycemia and hyperglycemic-induced oxidative stress. The use of lactic acid bacteria (LAB) is one of the new therapeutic strategies to improve diabetes and its complication. Thus, this paper aimed to investigate the anti-hyperglycemic and antioxidant properties of the cell-free extract (CFE) and cell-free supernatant (CFS) obtained from 10 native LAB strains isolated from traditional fermented dairy products. The anti-hyperglycemic activity of LAB strains was evaluated by the α-amylase and α-glucosidase inhibition assay. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl, superoxide anion, ferric-reducing, iron chelating, and total antioxidant capacity. Finally, phenolic and flavonoid content of CFS of samples were assessed. The findings showed that Lactobacillus delbrueckii had the higher antidiabetic properties compared to other strains with the highest α-glucosidase inhibitory activity in CFE (25%) and CFS (50%). Other LAB strains showed high antioxidant ability in CFE and CFS. Among them, Lactobacillus paracasei had the highest antioxidant activity. The results showed that the CFS contained phenolic and flavonoid compounds. Finally, the data demonstrated that L. delbrueckii, L. paracasei, Lactobacillus helveticus, and Lactobacillus fermentum could be applied in the production of health-related products, food additives and animal feed.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 2","pages":"331 - 346"},"PeriodicalIF":1.0,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140793266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Probiotic Attributes of Potential Probiotic Bacillus Strain Isolated from Indigenous Fermented Soybean Foods of Northeast, India 从印度东北部本土发酵大豆食品中分离出的潜在益生菌株的体外益生特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI: 10.1134/S0003683824020054

S. Huidrom, M. A. Beg, S. Nautiyal, S. Kalidas Singh, D. S. Huidrom

{"title":"In Vitro Probiotic Attributes of Potential Probiotic Bacillus Strain Isolated from Indigenous Fermented Soybean Foods of Northeast, India","authors":"S. Huidrom, M. A. Beg, S. Nautiyal, S. Kalidas Singh, D. S. Huidrom","doi":"10.1134/S0003683824020054","DOIUrl":"10.1134/S0003683824020054","url":null,"abstract":"This study aimed to isolate Bacillus species from indigenous non-salted fermented soybean of Northeast India to select a potential novel probiotic candidate. A total of 150 isolates were obtained and identified as Bacillus sp. from hawaijar (109) and kinema (41) based on phenotypic characterization. Antimicrobial activity was evaluated against the five pathogens such as Salmonella enterica typhimurium MTCC 98, Klebsiella pneumoniae MTCC 109, Shigella flexneri MTCC 1457, Pseudomonas aeruginosa MTCC 741, and Escherichia coli (clinical isolates). Forty five Bacillus isolates showed antagonism to at least 3 pathogens. In the antioxidant assay, the Bacillus isolates displayed varying degrees of scavenging activity which were in the range of 37.5–89.6%. In the hydrophobicity and autoaggregation tests, marked differences in cell hydrophobicity to xylene and auto-aggregation, ranging from 3.2 to 79.2% and from 34 to 97%, respectively, were noted among the tested strains. The most of the isolates revealed susceptibility to antibiotics except for nalidixic acid. In the hemolysis test, hemolytic strains were discarded and non-haemolytic strains were used for the in vitro test. Twenty five strains produced exopolysaccharides. In adhesion to Caco-2 cells, all the tested strains showed adhesion but with variation, Bacillus subtilis strain Kak2a showed the highest adhesion (2.34%). Based on the in vitro test, the best three isolates such as Pis3, Kak2a, and, Kw3 were selected as potential probiotics candidates. 16S rRNA sequence analyses were performed to confirm the identity of the selected three Bacillus isolates.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 2","pages":"301 - 314"},"PeriodicalIF":1.0,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140780643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Derivatives of Chitosan, Soluble in a Neutral Medium for Drugs and Genetic Material Carriers: Preparation and Properties 可溶于中性介质的药物和遗传物质载体壳聚糖功能衍生物：制备与性能

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI: 10.1134/S0003683824020030

M. Yu. Gorshkova, E. S. Gigoryan, I. F. Volkova

引用次数: 0