Applied Biochemistry and Microbiology最新文献_第10页

Peculiarities of the Yeast Microflora of Authentic Ossetia Cheeses 正宗奥塞梯奶酪酵母菌群的特点

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700206

A. Yu. Tuaeva, G. S. Kachmazov, E. S. Naumova

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Spheroids Based on Human Embryonic Lung Cell Line (HELC-3) and Their Use for the Accumulation of Herpes Simplex Virus Type 1 基于人胚胎肺细胞系（HELC-3）的球体及其在1型单纯疱疹病毒积累中的应用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700279

I. A. Ivashchenko, O. S. Fedotova, I. A. Korotkova, Y. A. Mikhailenko, V. V. Wasielewski, A. V. Ostapchuk, A. E. Panova

{"title":"Spheroids Based on Human Embryonic Lung Cell Line (HELC-3) and Their Use for the Accumulation of Herpes Simplex Virus Type 1","authors":"I. A. Ivashchenko, O. S. Fedotova, I. A. Korotkova, Y. A. Mikhailenko, V. V. Wasielewski, A. V. Ostapchuk, A. E. Panova","doi":"10.1134/S0003683825700279","DOIUrl":"10.1134/S0003683825700279","url":null,"abstract":"Traditional two-dimensional (2D) cell cultures have limited capabilities for modeling complex interactions between viruses and host cells, which reduces the efficiency of the studies when trying to transfer data obtained to in vivo models. Three-dimensional (3D) cell cultures are the most physiologically relevant models, without the limitations inherent to 2D cultures. The use of 3D models opens up new possibilities when studying viral infections and developing new antiviral drugs and preventive measures. We constructed a biological model based on 3D spheroids from the human embryonic lung cell line (HELC-3) and tested it on the example of the analysis of replicative activity of herpes simplex virus type 1, strain L2 (HSV-1/L2). The spheroids were obtained when cultivating the cells in hydrogel microwells. The replicative activity of HSV-1 in 3D culture of HELC-3 cells was estimated by a cytopathic effect of the culture liquid of virus-infected spheroids on a monolayer culture of the same cells (2D format). It was demonstrated that HSV-1 replicates efficiently in spheroids. Thus, the model based on 3D spheroids from HELC-3 cells can be considered as a promising tool for studying the HSV-1 replication cycle in vitro, molecular mechanisms of its pathogenesis and persistence, as well as the analysis of potential antiviral drugs and vaccines.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1508 - 1515"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145705963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Study of Degradation of Materials Based on Natural Silk Fabrics for Regenerative Medicine 基于天然丝织物的再生医学材料的体外降解研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S000368382570036X

E. I. Podbolotova, A. R. Pashutin, A. E. Efimov, O. I. Agapova, I. I. Agapov

{"title":"In Vitro Study of Degradation of Materials Based on Natural Silk Fabrics for Regenerative Medicine","authors":"E. I. Podbolotova, A. R. Pashutin, A. E. Efimov, O. I. Agapova, I. I. Agapov","doi":"10.1134/S000368382570036X","DOIUrl":"10.1134/S000368382570036X","url":null,"abstract":"The aim of this study was to create biodegradable scaffolds based on natural silk fabrics for regenerative medicine. Fabrics with different densities gas-chiffon (15 g/m2) and double-sided satin (155 g/m2) were used as matrices. The samples were preliminarily treated with a water–alcohol solution of calcium chloride for different periods of time to obtain the samples with different degrees of degradation. Morphological studies using light microscopy confirmed structural changes in the tissues after this treatment. Degradation of the obtained scaffolds was studied in vitro using Fenton’s reagent with different duration of incubation (15, 30, and 45 days). The rate of scaffold destruction depended on the type of fabric and time of preliminarily treatment with calcium chloride. The highest degradation rate was observed for gas-chiffon, while satin demonstrated slower destruction. The results obtained can be used when optimizing the properties of silk fabrics for their use in regenerative medicine and development of efficient methods to control their biodegradation.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1588 - 1591"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145705985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lux-Biosensors for Analyzing the Intracellular Pool of Macroergic Compounds and Reducing Equivalents 用于分析细胞内巨能化合物和还原等价物的lux生物传感器

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700255

R. N. Al Ebrahim, S. V. Bazhenov, M. Yu. Vyssokikh, Y. E. Liu, A. D. Vlasova, S. M. Bukhalovich, I. V. Manukhov

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Analysis of the Profile of Secreted Proteolytic Enzymes of the Micromycete Aspergillus ochraceus VKM F-4104D 微霉菌ochraceaspergillus VKM F-4104D分泌蛋白水解酶谱分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S000368382570005X

E. A. Popova, A. A. Shestakova, D. E. Surkova, A. A. Osmolovskiy

{"title":"Analysis of the Profile of Secreted Proteolytic Enzymes of the Micromycete Aspergillus ochraceus VKM F-4104D","authors":"E. A. Popova, A. A. Shestakova, D. E. Surkova, A. A. Osmolovskiy","doi":"10.1134/S000368382570005X","DOIUrl":"10.1134/S000368382570005X","url":null,"abstract":"The enzymes that are least represented but most in demand in biotechnology are highly specific proteases that can hydrolyze difficult-to-degrade substrates. Micromycetes from the genus Aspergillus have been identified as producers of these proteases, which have diverse biochemical properties. For Aspergillus ochraceus VKM F-4104D, which has been previously studied as a producer of a protease that activates protein C and human factor X, its ability to secrete proteolytic enzymes that act on globular and fibrillar proteins has been investigated when cultured in nine different nutrient media with varying nitrogen sources. Maximum collagenolytic and fibrinolytic activity was observed when using a medium containing bovine collagen as an inducer. During preparative isoelectric focusing and subsequent zymography, two proteases with isoelectric points of 5.6 and 6.2 and molecular weights of 74 and 33 kilodaltons, respectively, were identified. As a result of genomic analysis of A. ochraceus VKM F-4104D, 121 potential secreted proteases have been predicted. Transcriptomic analysis revealed that when grown on a medium containing bovine collagen with high proteolytic activity, the microorganism expresses 117 extracellular proteases. Of these, only two were identified using traditional enzymological methods. Thus, the use of more advanced techniques allows for the detection of previously unidentified proteases with different activities among organisms for which information on physiology has been gathered or individual enzymes have been described previously, whereas traditional methods for identifying new enzymes often yield sparse or unrepresentative results.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1287 - 1295"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of α-Amylase Bacillus amyloliquefaciens in the Expression System of Bacillus mojavensis and Properties of the Recombinant Enzyme 莫氏芽孢杆菌表达体系中α-淀粉酶解淀粉芽孢杆菌的产生及重组酶的性质

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700103

V. Yu. Chirkova, D. N. Shcherbakov, P. V. Kolosov, M. V. Shirmanov, E. A. Kolosova, A. V. Malkova, E. A. Sharlaeva, A. N. Irkitova, I. Yu. Evdokimov

{"title":"Production of α-Amylase Bacillus amyloliquefaciens in the Expression System of Bacillus mojavensis and Properties of the Recombinant Enzyme","authors":"V. Yu. Chirkova, D. N. Shcherbakov, P. V. Kolosov, M. V. Shirmanov, E. A. Kolosova, A. V. Malkova, E. A. Sharlaeva, A. N. Irkitova, I. Yu. Evdokimov","doi":"10.1134/S0003683825700103","DOIUrl":"10.1134/S0003683825700103","url":null,"abstract":"The conditions of recombinant ɑ-amylase production in an induction-free cultivation system based on Bacillus mojavensis are described. Fermentation was carried out in pilot fermenters under deep cultivation, which ensured the production of experimental batches in the quantities necessary for comprehensive studies of the samples obtained. Gradual accumulation of the recombinant enzyme in the bioreactor reaches a maximum after 31 hours. Some biochemical parameters of the recombinant enzyme were studied. Its thermostability was established, and the pH optimum of activity manifestation was determined—the maximum efficiency of substrate hydrolysis was observed when the sample was preheated for 30 min at a temperature of 55°C and pH equal to 7.2. An inversely proportional relationship between the change in the level of amylolytic activity and the amount of calcium chloride added during cultivation was revealed. The possibility of creating strains producing recombinant ɑ-amylase on the basis of B. mojavensis strain BDV-1 was demonstrated.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1335 - 1342"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryogenically Structured Wide-Porous Gelatin–Hyaluronate Carrier of Peptide Bioregulator 低温结构宽孔明胶透明质酸多肽生物调节剂载体

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700127

E. V. Sidorskii, M. S. Krasnov, V. I. Lozinsky

{"title":"Cryogenically Structured Wide-Porous Gelatin–Hyaluronate Carrier of Peptide Bioregulator","authors":"E. V. Sidorskii, M. S. Krasnov, V. I. Lozinsky","doi":"10.1134/S0003683825700127","DOIUrl":"10.1134/S0003683825700127","url":null,"abstract":"Using cryostructuring techniques, a new biocompatible, wide-porous gelatin-hyaluronate material was prepared, which was successfully tested as a carrier of a protein-peptide bioregulator. The following physicochemical parameters of the synthesized carrier were determined: the total water-holding capacity (51.2 ± 5.8 g H2O/g polymer), the degree of swelling (7.03 ± 0.65 g H2O/g polymer) of the polymer inside the macropore walls of the spongy material and the compressive modulus of elasticity (12.3 ± 1.5 kPa). The presence of a system of interconnected macropores with a cross section from 20 to 210 μm in the material is shown. Next, the resulting carrier was loaded with a protein-peptide bioregulator isolated from bovine sclera and used in the experiments on organ cultivation of a biological model: the posterior section of the newt eye. As a result, a significant protective effect of the scleral bioregulator included in the sponge carrier on the viability of tissues and cells of a biological model was revealed and the prospects of a new biocompatible cryogenically structured material for dosed release of the bioregulator into the culture medium was shown.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1353 - 1360"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Action of Microvesicules on Rat Spermatozoa In Vitro 微囊泡对体外大鼠精子的保护作用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700139

A. M. Aimaletdinov, A. G. Malanyeva, M. A. Tambovsky, E. Y. Zakirova

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Production of a Recombinant Cutinase from Fusarium solani and Estimate of Its Ability to Enhance the Glyphosate Effect 从茄枯萎菌中提取重组角质酶的制备及其增强草甘膦效应的能力评价

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700024

D. O. Osipov, E. A. Rubtsova, I. A. Shashkov, A. D. Satrutdinov, A. M. Rozhkova, L. A. Shcherbakova, M. V. Plaksina, A. P. Sinitsyn

{"title":"Production of a Recombinant Cutinase from Fusarium solani and Estimate of Its Ability to Enhance the Glyphosate Effect","authors":"D. O. Osipov, E. A. Rubtsova, I. A. Shashkov, A. D. Satrutdinov, A. M. Rozhkova, L. A. Shcherbakova, M. V. Plaksina, A. P. Sinitsyn","doi":"10.1134/S0003683825700024","DOIUrl":"10.1134/S0003683825700024","url":null,"abstract":"A P. verruculosum strain producing Fusarium solani extracellular cutinase CUT with a high level of expression (more than 40%) was obtained by cloning the cutB gene in the recipient strain. The homogeneous CUT protein with high pNPB enzymatic activity (1070 U/mg) was purified by chromatographic methods. The homogeneous cutinase had a temperature optimum at 55°C and a pH optimum at 8. CUT retained more than 80% activity for 30 min at 50°C. About 50% activity was retained for 3 min at 60°C, and the enzyme completely lost activity in 1 min at 70°C. CUT EP had no significant phytotoxic effect, but its enzymatic activity facilitated the penetration of herbicide into plant tissues, which was expressed in a greater proportion of treated leaves with signs of glyphosate injuries. Joint application of CUT EP and herbicide resulted in a synergistic effect and allowed reducing the herbicide dosage five times, while the effectiveness of the latter decreased only two times. The selection of more effective combinations of CUT EP with glyphosate to reduce its doses without losing the exterminating effect will reduce the medical and environmental risks of using this herbicide.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1263 - 1271"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of the Content of Macro- and Microelements in the Culture Medium on the Productivity of Withania coagulans Hairy Root Cultures 培养基中宏、微量元素含量对凝固Withania毛状根培养产量的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-12-09 DOI: 10.1134/S0003683825700012

E. V. Mikhaylova, M. A. Panfilova, V. V. Fedyaev, B. R. Kuluev

{"title":"The Impact of the Content of Macro- and Microelements in the Culture Medium on the Productivity of Withania coagulans Hairy Root Cultures","authors":"E. V. Mikhaylova, M. A. Panfilova, V. V. Fedyaev, B. R. Kuluev","doi":"10.1134/S0003683825700012","DOIUrl":"10.1134/S0003683825700012","url":null,"abstract":"Withania coagulans (Stocks) Dunal is a valuable medicinal plant with prospects for use not only in medicine, but also in the food industry. The hairy roots of withania are a more promising source of biologically active substances than the roots of plants grown in natural conditions. There is evidence of a positive effect of the compounds KNO3, KH2PO4, MgSO4, and CaCl2 on the growth of the roots of Withania somnifera (L.) Dunal, cobalt salts on the growth of the roots of Angelica archangelica L., and increased agar concentration on the growth of the roots of A. thaliana. In this work, the effect of these compounds on the growth of hairy roots of W. coagulans was studied for the first time. It has been shown that changing the composition of the MS medium does not contribute to an increase in the dry mass of the roots. An increase in the concentration of divalent cobalt inhibits root growth, while trivalent cobalt in a concentration of up to 100 μM prolongs the viability of roots. The addition of [Co(NH3)]6Cl3 can be used for long-term storage of withania root cultures without passages.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1255 - 1262"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0