Applied Biochemistry and Microbiology最新文献_第10页

A Novel Organic Solvent Tolerant NAD+-dependent Formate Dehydrogenase from Halophilic Yeast Candida diddensiae 来自嗜卤酵母菌 Candida diddensiae 的新型耐有机溶剂 NAD+依赖性甲酸脱氢酶

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S000368382460012X

G. Kurt-Gür, E. Arslan, Ö. Başsaraç, E. Ordu

{"title":"A Novel Organic Solvent Tolerant NAD+-dependent Formate Dehydrogenase from Halophilic Yeast Candida diddensiae","authors":"G. Kurt-Gür, E. Arslan, Ö. Başsaraç, E. Ordu","doi":"10.1134/S000368382460012X","DOIUrl":"10.1134/S000368382460012X","url":null,"abstract":"Characterization of new halophilic enzymes capable of thriving in non-aqueous biocatalysis of significant interest in the non-aqueous chemoenzymatic processes. Despite the number of studies dealing with increasing the stability of NAD+-dependent formate dehydrogenases (FDHs), there are few studies characterizing their behavior in organic solvents. FDHs have a great advantage in cofactor regeneration systems and the reduction of CO2 to formate. It is important to find novel FDHs for the wide application field of organic chemicals. In this study, a novel NAD+-dependent formate dehydrogenase from Candida diddensiae YLP9 (CdFDH), isolated from olive brine and having tolerance under low pH and high salt concentration, was heterologously expressed and characterized. Recombinant CdFDH has optimum activity at pH 7.0–8.0 when the substrate is formate. The midpoint of thermal inactivation (T0.5) for CdFDH was calculated as 54°C. The enzyme shows high stability at рН 6.0 and 53°С ((k_{{{text{in}}}}^{{{text{ef}}}}) 0.28 × 10–4 /s). Comparing the enzyme activity results obtained from incubation of enzyme in media without salt and with different concentrations of NaCl, KCl, and MgCl2 revealed that concentrations of NaCl and KCl between 0.4–4.0 M contribute to proper folding and activity of CdFDH. Enzyme activity was preserved and significantly increased after incubating in the 20–80% of DMSO, 40% 3-methyl butanol, 70% n-butanol and, all tested concentrations of n-hexane. The results show that NAD+-dependent CdFDH is a promising candidate biocatalyst to use in non- or low-aqueous biotechnological applications.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1162 - 1170"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Antimicrobial Activity in Fungi-destructors of Tempera Painting Isolated in the State Tretyakov Gallery 测定国立特列季亚科夫美术馆分离出的钢笔画真菌破坏者的抗菌活性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824605742

A. A. Ermolyuk, D. A. Avdanina, F. S. Koblov, S. G. Kalinin, T. V. Kolganova, B. F. Vasilieva, M. V. Demiankova, O. V. Efremenkova, A. A. Zghun

{"title":"Determination of Antimicrobial Activity in Fungi-destructors of Tempera Painting Isolated in the State Tretyakov Gallery","authors":"A. A. Ermolyuk, D. A. Avdanina, F. S. Koblov, S. G. Kalinin, T. V. Kolganova, B. F. Vasilieva, M. V. Demiankova, O. V. Efremenkova, A. A. Zghun","doi":"10.1134/S0003683824605742","DOIUrl":"10.1134/S0003683824605742","url":null,"abstract":"In current work, we for the first time studied the antimicrobial activity of representatives of mold fungi, which occupy a rather specific ecological niche, since they are destructors of tempera painting materials. We screened 24 molds that had previously been isolated from exhibits and in the halls of the State Tretyakov Gallery and for which the ability to destroy materials used in tempera painting and scientific restoration was shown. Screening for antimicrobial activity was carried out against a panel of 16 test cultures, characteristic for identifying activities of various types. The strains exhibited activity, including against Staphylococcus aureus INA 00761 (MRSA), S. aureus FDA 209P (MSSA), Pseudomonas aeruginosa ATCC 27853, Mycobacterium smegmatis VKPM Ac-1339, M. smegmatis mc2 155 and Leuconostoc mesenteroides VKPM B-4177. Among the studied paint-degrading fungi, strains Simplicillium lamellicola STG-96, Cerarobasidium sp. STG-324, Penicillium sp. STG-327 and Penicillium sp. STG-344 turned out to be the most promising for further research related to the determination what specific highly active compounds they produce.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1266 - 1274"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of Recombinant Endolysin LysSi3 to Increase its Antibacterial Properties 对重组内溶解素 LysSi3 进行工程改造以增强其抗菌特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S000368382460492X

N. P. Antonova, I. V. Grigoriev, A. M. Lendel, O. V. Usacheva, A. A. Klimova, E. V. Usachev, V. A. Gushchin, D. V. Vasina

引用次数: 0

Development of a Bio-Selecting Agent Based on Immobilized Bacterial Cells with Amidase Activity for Bio-Detection of Acrylamide 开发基于具有酰胺酶活性的固定化细菌细胞的生物选择剂，用于生物检测丙烯酰胺

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824605031

E. M. Protasova, Yu. G. Maksimova

{"title":"Development of a Bio-Selecting Agent Based on Immobilized Bacterial Cells with Amidase Activity for Bio-Detection of Acrylamide","authors":"E. M. Protasova, Yu. G. Maksimova","doi":"10.1134/S0003683824605031","DOIUrl":"10.1134/S0003683824605031","url":null,"abstract":"Actinobacteria cells Rhodococcus erythropolis 4-1 and Rhodococcus erythropolis 11-2 and Proteobacteria Alcaligenes faecalis 2, which have amidase activity, were immobilized by entrapping barium alginate and agarose into the gel structure, as well as by obtaining biofilms on thermally expanded graphite (TEG). The operational stability of such immobilized biocatalysts after storage in frozen and dehydrated form was determined, and a prototype of a conductometric acrylamide biosensor based on such a bioselective agent was developed. The most preferred method for storing immobilized cells was freezing at temperatures from –20 to –80°C; long-term storage is also possible wet at 4–25°C. It was shown that these cells were most preferable for the biodetection of acrylamide A. faecalis 2, immobilized in an agarose gel structure. An agarose gel with bacterial cells immobilized in its structure had greater mechanical strength and stability during successive cycles of conversion of acrylamide into acrylic acid compared to barium alginate gel. The mechanical strength of barium alginate gel can be enhanced by the addition of carbon nanomaterials during cell immobilization. Growing biofilms on carbon materials used for manufacturing electrodes is also promising. Biofilms of R. erythropolis 11-2 on TEG are capable of converting acrylamide into acrylic acid in more than 20 reaction cycles while maintaining at least 50% amidase activity.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"942 - 949"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683824605031.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABA-Degrading Strains of Bacteria of the Genus Pseudomonas and Their Influence on Wheat Growth 假单胞菌属细菌的 ABA 降解菌株及其对小麦生长的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604566

A. S. Ryabova, L. Yu. Kuzmina, N. F. Galimsyanova, E. A. Gilvanova, L. B. Vysotskaya, E. V. Martynenko, G. R. Kudoyarova

{"title":"ABA-Degrading Strains of Bacteria of the Genus Pseudomonas and Their Influence on Wheat Growth","authors":"A. S. Ryabova, L. Yu. Kuzmina, N. F. Galimsyanova, E. A. Gilvanova, L. B. Vysotskaya, E. V. Martynenko, G. R. Kudoyarova","doi":"10.1134/S0003683824604566","DOIUrl":"10.1134/S0003683824604566","url":null,"abstract":"Three new representatives of the genus Pseudomonas have been identified that are capable of utilizing abscisic acid and positively influencing the growth and development of plants. Their physiological and biochemical properties have been studied. Based on analysis of the 16S rRNA gene, they were identified as P. veronii IB K11-1 (99.86% similarity) and P. frederiksbergensis IB Ta10m (100%), and strain TaE2 was assigned to Pseudomonas sp. It was found that bacteria, when grown on a mineral-salt medium with ABA, reduced the hormone content by 50–60% with an increase in population density by two orders of magnitude. In a laboratory experiment, it was shown that the introduction of the bacterial biomass (108 CFU/g of substrate) into the rhizosphere of wheat plants ten days after treatment led to a decrease in the abscisic acid content in the roots by 18–30% and an increase in plant weight by up to 30%. Thus, new strains of growth-stimulating ABA-degrading bacteria have been identified and characterized for the first time, which may be promising for the creation of biological products that increase plant resistance to biotic and abiotic stresses.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"925 - 930"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains (Review) 支原体：细胞培养物和病毒株的特性、检测和净化方法（综述）

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604943

O. A. Leonovich

{"title":"Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains (Review)","authors":"O. A. Leonovich","doi":"10.1134/S0003683824604943","DOIUrl":"10.1134/S0003683824604943","url":null,"abstract":"Mycoplasma contamination of continuous cell cultures and collection viral strains remains a serious problem in the biotechnology industry and experimental research. The frequency of mycoplasma contamination of cultured cell lines and viruses is 15–35%, and in some cases up to 80%. Mycoplasmas cause various changes in cultures contaminated by them, including cell death. They have immunomodulatory properties and affect the yield of certain viruses propagated in cell cultures. Mycoplasmas do not have a cell wall, are able to pass through a bacterial filter, have the smallest genome (≈580 kb) among bacteria, and are capable of independent reproduction and existence. These microorganisms are resistant to most antibiotics commonly used in cell culture. Derivative groups of tetracyclines and fluoroquinolones (BM-Cyclin®, Ciprobay®, Baytril®, Plasmocin®, MRA) have shown certain effectiveness in decontaminating viral strains and cell cultures from mycoplasmas. Timely, highly sensitive detection and prevention of mycoplasma infection is of great importance. For routine scanning of mycoplasma infection of continuous cell cultures and viral strains, the methods of indicator cell culture (cytochemical) and polymerase chain reaction (PCR) are recommended, or for more accuracy, microbiological analysis of mycoplasma colonies on a special medium.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"785 - 793"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for Preprocessing Reeds to Obtain Enzymatic Hydrolysates with a High Sugar Content 预处理芦苇以获得含糖量高的酶水解物的方法

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604736

M. V. Semenova, A. M. Rozhkova, D. O. Osipov, V. D. Telitsin, E. A. Rubtsova, E. G. Kondrat’eva, I. S. Vasil’eva, O. V. Morozova, A. I. Yaropolov, A. P. Sinitsyn

{"title":"Methods for Preprocessing Reeds to Obtain Enzymatic Hydrolysates with a High Sugar Content","authors":"M. V. Semenova, A. M. Rozhkova, D. O. Osipov, V. D. Telitsin, E. A. Rubtsova, E. G. Kondrat’eva, I. S. Vasil’eva, O. V. Morozova, A. I. Yaropolov, A. P. Sinitsyn","doi":"10.1134/S0003683824604736","DOIUrl":"10.1134/S0003683824604736","url":null,"abstract":"Methods of the pretreatment of reeds (grinding, hydrothermal treatment, treatment with acid or alkali solutions, organosolv, deep eutectic solvents) and their effect on the subsequent enzymatic hydrolysis by cellulase and hemicellulase complexes have been studied. Substrates with the highest reactivity were obtained by exposing the reeds to a weakly alkaline deep eutectic solvent (DES) and an alkali solution. The depth of enzymatic hydrolysis of these pretreated substrates was 63 and 59%, and the degree of conversion of polysaccharides (cellulose and hemicellulose) into monosaccharides taking into account the yield of the substrate at the preprocessing stage was 60 and 34%, respectively. After pretreatment of the reeds with acid DES, water steam, water, or a water/organic solution of sulfuric acid, the depth of enzymatic hydrolysis was 45, 25, 20, and 11% and the degree of polysaccharide conversion was 26, 18, 13, and 10%, respectively. The industrial enzyme preparation Agrocell Plus with a predominant content of cellobiohydrolases and endoglucanases was most effective in hydrolyzing the dust fraction of the reed, as was the reed pretreated with a solution of sulfuric acid or DES (acidic or alkaline). The industrial enzyme preparation Agroxil Plus, containing endoxylanase and cellobiohydrolases, was more effective in hydrolyzing the reed after hydrothermal pretreatment or alkali solution. The results of the hydrolysis of the reed pretreated with acidic or weakly alkaline DES under the action of individual (homogeneous) cellulases indicate that in both cases the key enzyme was cellobiohydrolase 1.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"931 - 941"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospects of Acoustic Sensor Systems for Virus Immunodetection 声学传感器系统在病毒免疫检测中的应用前景

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604748

O. I. Guliy, B. D. Zaitsev, O. A. Karavaeva, I. A. Borodina

引用次数: 0

Effect of Different Sucrose Concentrations on the Biosynthesis of Poly(3-Hydroxybutyrate) and Alginate by the Bacterial Strain Azotobacter Vinelandii 12 under Different Aeration Conditions 不同通气条件下不同蔗糖浓度对葡萄氮杆菌 12 菌株生物合成聚（3-羟基丁酸）和藻酸盐的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604876

A. A. Dudun, T. K. Makhina, A. P. Bonartsev, G. A. Bonartseva

{"title":"Effect of Different Sucrose Concentrations on the Biosynthesis of Poly(3-Hydroxybutyrate) and Alginate by the Bacterial Strain Azotobacter Vinelandii 12 under Different Aeration Conditions","authors":"A. A. Dudun, T. K. Makhina, A. P. Bonartsev, G. A. Bonartseva","doi":"10.1134/S0003683824604876","DOIUrl":"10.1134/S0003683824604876","url":null,"abstract":"Bacteria of the genus Azotobacter sp. produce two classes of biologically important biocompatible and biodegradable polymers, polyoxyalkanoates, which are the bacterial reserve, and alginates (ALG), which perform the function of protecting nitrogenase from oxygen. Both polymers are becoming increasingly important for use in bioengineering, pharmaceuticals, and medicine, so studies of their biosynthesis and properties are currently highly relevant. The present work shows the possibility of regulating alginate and poly(3-hydroxybutyrate) (PHB) synthesis by a culture of A. vinelandii 12 depending on the increase in the sucrose concentration in the medium under different aeration conditions. At high aeration and high sucrose concentrations in the medium (50 g/L), the maximum yield of free (1.08 g/L) and capsular ALG (2.26 g/L) in the medium was obtained. Under low aeration conditions, the synthesis of free ALG was completely inhibited. The maximum value of РНB synthesis was observed at medium aeration and a high concentration of sucrose (50 g/L) in the medium. The maximum molecular weight (MW) of ALG was 477 kDa, while the maximum MW of PHB was much higher, reaching 1479 kDa. At low sucrose concentrations in the medium (5 to 20 g/L), capsular ALG is predominantly synthesized (up to 100% of the sum of all polymers) at all aeration levels. With increasing sucrose concentration, PHB is predominantly synthesized (68%) under low aeration conditions, an equal ratio of PHB and capsular ALG synthesis is observed under medium aeration conditions, and free ALG is actively synthesized under high aeration conditions. This work demonstrates the possibility of obtaining a selective synthesis of ALG or PHB by A. vinelandii 12 by modifying its cultivation conditions. The results obtained can be used for the development of directed biosynthesis of target products (PHB and ALG) in biotechnology.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"821 - 832"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An In Vivo Method for Biotinylation of Recombinant Variola Virus Proteins 生物素化重组变异病毒蛋白质的体内方法

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824605201

V. N. Nikitin, Yu. A. Merkuleva, D. N. Shcherbakov

引用次数: 0