Applied Biochemistry and Microbiology最新文献_第10页

Erratum to: Biosynthesis of Suberiс Acid from Glucose through Inverted Fatty Acid β-Oxidation by Recombinant Escherichia coli Strains 重组大肠杆菌菌株通过倒置脂肪酸β-氧化从葡萄糖合成亚硒酸的研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825090066

A. Yu. Gulevich, A. Yu. Skorokhodova, V. G. Debabov

引用次数: 0

Evaluation of the Effectiveness of Fluorescent Labeling of Cells and Microvesicles with the Katushka2S Protein to Assess Their Biodistribution In Vivo and Ex Vivo Katushka2S蛋白荧光标记细胞和微泡在体内和离体生物分布的有效性评价

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825700565

V. A. Kiryanova, A. M. Aimaletdinov, S. K. Kletukhina, M. O. Gomzikova

{"title":"Evaluation of the Effectiveness of Fluorescent Labeling of Cells and Microvesicles with the Katushka2S Protein to Assess Their Biodistribution In Vivo and Ex Vivo","authors":"V. A. Kiryanova, A. M. Aimaletdinov, S. K. Kletukhina, M. O. Gomzikova","doi":"10.1134/S0003683825700565","DOIUrl":"10.1134/S0003683825700565","url":null,"abstract":"A comparative in vivo analysis was conducted to evaluate the biodistribution efficiency of cells and microvesicles labeled with fluorescent proteins. Mesenchymal stem cells (MSCs) were genetically modified using a recombinant lentivirus carrying the gene for the fluorescent protein Katushka2S, with the goal of labeling both the cells and their derived microvesicles (MVs). The selection of genetically modified MSCs was performed through cell sorting using a FACS Aria III flow cytometer–sorter. The biodistribution of the cells was assessed by detecting the fluorescent signal using the IVIS Spectrum system. Intravenous administration of 5 × 105 MSC-Katushka2S in mice was insufficient for in vivo detection of cell biodistribution. Ex vivo analysis revealed that immediately after intravenous administration, the MSCs accumulated in the lungs and liver. The intensity of Katushka2S protein fluorescence and the sensitivity of the IVIS Spectrum system were sufficient for ex vivo visualization of MSC-Katushka2S cell clusters. However, the quantity of MVs-Katushka2S isolated from 1.5 × 106 MSC-Katushka2S was insufficient for the detection of MV biodistribution both in vivo and ex vivo. Therefore, the application of this method for evaluating the biodistribution of individual cells and microvesicles in vivo is ineffective.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 9","pages":"1743 - 1752"},"PeriodicalIF":1.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and Substantiation of Methods for Estimating the Consumer Properties of Cosmetic Products by Deodorant and Antiperspirant Effect. Part 2. Development of an Express Method for Estimating the Intrinsic Antimicrobial Activity of the Products 用除臭剂和止汗剂评价化妆品消费者性能的方法的选择和证实。第2部分。产品固有抗菌活性快速评价方法的建立

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825700656

Yu. A. Penkina, V. R. Normatova, T. T. N. Ha, I. A. Butorova

{"title":"Selection and Substantiation of Methods for Estimating the Consumer Properties of Cosmetic Products by Deodorant and Antiperspirant Effect. Part 2. Development of an Express Method for Estimating the Intrinsic Antimicrobial Activity of the Products","authors":"Yu. A. Penkina, V. R. Normatova, T. T. N. Ha, I. A. Butorova","doi":"10.1134/S0003683825700656","DOIUrl":"10.1134/S0003683825700656","url":null,"abstract":"The intrinsic antimicrobial activity of personal care products against the sweat odor is a necessary element of their functional properties. To estimate this characteristic, two bacterial strains typical for the armpit zone were selected as test organisms: Corynebacterium stationis VKPM-V-10645 and Micrococcus luteus VKPM-V-7845 that demonstrated a sensitivity to all tested commercial deodorant/antiperspirant samples. An express method (a method of “rapid” microbiology) using a 0.03% water solution of 2,3,5-triphenyltetrazolium chloride (TTC) was suggested. The testing conditions were selected: the amount of test strain inoculum introduced into the reaction medium, as well as the amount and method of introducing a cosmetic product for significant accounting of the results after 3 h of incubation of the samples. This express method can be used for rapid estimation of antimicrobial activity and recommended for the analysis of large volume of objects, particularly, when searching or synthesizing new active biocidal ingredients and in large-scale testing of cosmetics against the sweat odor.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 9","pages":"1823 - 1831"},"PeriodicalIF":1.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: Impact of Neurotransmitters on the Photosynthetic Pigment Content of the Green Microalga Haematococcus lacustris (Strains IPPAS H-239 and BM-1) 神经递质对绿色微藻湖红球藻（菌株IPPAS H-239和BM-1）光合色素含量的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S000368382509011X

Cao Boyang, T. A. Fedorenko, O. B. Chivkunova, A. E. Solovchenko, E. S. Lobakova, A. V. Oleskin

引用次数: 0

Erratum to: Degradation of Dibutyl Phthalate by the Halotolerant Strain Pseudarthrobacter sp. NKDBFgelt 耐盐假关节杆菌sp. NKDBFgelt降解邻苯二甲酸二丁酯的勘误

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S000368382509008X

O. V. Yastrebova, A. A. Pyankova, A. V. Nazarov, Yu. I. Nechaeva, E. S. Korsakova, E. G. Plotnikova

引用次数: 0

Biodegradable Composite Materials Based on Natural Polymers 基于天然聚合物的可生物降解复合材料

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825700541

V. A. Demina, A. A. Stupnikov, P. M. Gotovtsev, S. V. Krashenninikov, S. N. Chvalun

引用次数: 0

Monte Carlo Method for Calculating the Probability of Monoclonality of Cell Lines 计算细胞系单克隆概率的蒙特卡罗方法

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825700620

S. S. Timonova, V. I. Pavelko, I. A. Kiric, V. N. Bade, A. A. Piskunov, R. A. Khamitov

引用次数: 0

Erratum to: Probiotic Properties of Lactobacillus helveticus Isolated from Kefir Grains and Feces of Homo sapiens 从开菲尔颗粒和智人粪便中分离的helveticus乳杆菌益生菌特性的勘误

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825090054

O. S. Savinova, A. V. Shabaev, T. V. Fedorova

引用次数: 0

Erratum to: Synthesis and Antibacterial Activity of Silver Nanoparticles Stabilized by Lipopeptides and Glycolipids Produced by Bacillus amyloliquefaciens and Pseudomonas fluorescens 解淀粉芽孢杆菌和荧光假单胞菌产生的脂肽和糖脂稳定银纳米颗粒的合成和抗菌活性

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825090078

A. G. Khina, A. S. Gordeev, L. R. Biktasheva, D. M. Gorbunov, P. A. Kuryntseva, G. V. Lisichkin, Yu. A. Krutyakov

引用次数: 0

Downregulation of ELOVL5 Gene Expression Alters the Mechanism of Ferroptosis in MDA-MB-231 Breast Cancer Cells ELOVL5基因表达下调改变MDA-MB-231乳腺癌细胞铁下垂机制

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825700589

A. V. Razumovskaya, T. A. Kulagin, A. A. Fatkulin, M. O. Silkina, D. M. Olkhovik, S. V. Nikulin

{"title":"Downregulation of ELOVL5 Gene Expression Alters the Mechanism of Ferroptosis in MDA-MB-231 Breast Cancer Cells","authors":"A. V. Razumovskaya, T. A. Kulagin, A. A. Fatkulin, M. O. Silkina, D. M. Olkhovik, S. V. Nikulin","doi":"10.1134/S0003683825700589","DOIUrl":"10.1134/S0003683825700589","url":null,"abstract":"To date, breast cancer is the most common oncological pathology among the female population both in Russia and worldwide. Previously, our laboratory team identified a significant prognostic marker for breast cancer, namely the ELOVL5 gene, the expression level of which coupled with the IGFBP6 gene allowed one to predict with high sensitivity (82%) and specificity (62.5%) the risk of breast cancer recurrence during the first five years of observation. At the same time, low expression of these genes in tumor tissue corresponded to an unfavorable prognosis. ELOVL5 is a human elongase of long-chain polyunsaturated fatty acids (LC-PUFA), the use of which is often associated with antitumor effects due to the induction of ferroptotic cell death associated with accelerated peroxidation of intracellular lipids. Thus, activation of ferroptosis, an alternative pathway of programmed cell death, in tumor cells is considered a promising approach to overcome drug resistance. Here, changes in the dynamics of ferroptotic cell death, as well as the transcriptome profile of breast cancer cells with decreased ELOVL5 gene expression were investigated.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 9","pages":"1760 - 1769"},"PeriodicalIF":1.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0