Applied Biochemistry and Microbiology最新文献

{"title":"Actinobacteria: Antiviral and Phytostimulating Activity","authors":"Y. V. Bataeva,&nbsp;L. N. Grigoryan","doi":"10.1134/S0003683825700346","DOIUrl":"10.1134/S0003683825700346","url":null,"abstract":"<p>The phytotoxicity and antiviral activity of three strains of actinobacteria, <i>Streptomyces carpaticus</i> RCAM04697 (SCPM-O-B-9993), <i>Nocardiopsis umidischolae</i> RCAM04882, and <i>Nocardiopsis umidischolae</i> RCAM04883, were studied. For seven days, the bacterial cells were cultivated on starch–casein, potato–glucose, or Gause’s medium. Using immune-chromatography assay, we showed all three Actinobacteria strains exhibiting antiviral activity against Cucumber mosaic virus (CMV) on tomato plants. The maximum efficiency (100%) against CMV was found for a three-day suspension of the <i>S. carpaticus</i> strain RCAM04697 (SCPM-O-B-9993) on all nutrient media. Suspensions of all three strains obtained by cultivating for 1, 2, 6, and 7 days did not inhibit replication of the phytovirus.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1573 - 1580"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145705983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Chitosan Lactate Suitable for Stopping Intracavitary Bleeding","authors":"M. V. Volkova,&nbsp;A. M. Nosov,&nbsp;K. P. Golovko,&nbsp;V. A. Matsura,&nbsp;K. N. Demchenko,&nbsp;A. Ya. Kovalevsky,&nbsp;Ya. B. Kovalevsky","doi":"10.1134/S0003683825700358","DOIUrl":"10.1134/S0003683825700358","url":null,"abstract":"<p>A comparative assessment of the water absorption and hemostatic activity of chitosan lactate gel in vitro was carried out. IR spectrophotometry and thermogravimetric analysis confirmed the production of chitosan salt. The hemostatic activity of chitosan lactate gel was confirmed by in vitro experiments. The results obtained in vitro were correlated with previously published preliminary results on stopping intracavitary bleeding in vivo.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1581 - 1587"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145705984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Component Composition of the Indigenous Ethnos Gene Pool of Dagestan","authors":"M. O. Radzhabov,&nbsp;V. N. Kharkov,&nbsp;N. A. Kolesnikov,&nbsp;A. A. Zarubin,&nbsp;I. Yu. Khitrinskaya,&nbsp;M. A. Dzhaubermezov,&nbsp;O. M. Radzhabov,&nbsp;E. K. Khusnutdinova,&nbsp;V. A. Stepanov","doi":"10.1134/S0003683825700395","DOIUrl":"10.1134/S0003683825700395","url":null,"abstract":"<p>A component analysis of the gene pool composition of the indigenous ethnic groups of Dagestan, as well as other ethnic groups of the North Caucasus and Central Asia, was carried out based on wide-genome genotyping of SNP markers on the high-density DNA microarrays Infinium Multi-Ethnic Global-8. The analysis of the genotypic data array of ethnic populations of the above-mentioned regions was implemented in the Admixture algorithm (K = 12). Despite the fact that in ethno-linguistic terms, the Mingrelians belong to the people of the Kartvelian language family, the Abkhazians, Adyghe, and Circassians belong to the group of ethnic groups of the Abkhaz–Adyghe languages. The Ossetians are an Iranian-speaking ethnic group, and the Karachais and Balkars represent the Turkic group of peoples of the North Caucasus. All of the ethnic groups listed have a spectrum of the same ancestral components, moreover, in comparable proportions. The Ingush and Chechens, being representatives of the Nakh group of the Nakh–Dagestani language family, demonstrate one common ancestral component with a high degree of representation, emphasizing the isolated genetic history of the formation of their gene pool. The Karanogais of Dagestan and the Nogais of Karachai-Cherkessia, in terms of the spectrum and frequency of representation of ancestral components, indicate a commonality with Central Asian ethnic groups, such as the Kazakhs and Uzbeks. The ethnic groups of Dagestan demonstrate a unique picture of diversity in both the composition and frequency of occurrence of ancestral components in the structure of their autosomal gene pool. This emphasizes not only the history of their formation, isolated from other peoples of the North Caucasus, but also the long-isolated genetic history of each indigenous ethnic group. This study is conducted within the framework of fundamental areas of human population and evolutionary genetics, identifying ethnospecific features of the structure of genomes and population gene pools of modern human populations and reconstructing ancient migrations and population expansion, as well as the evolution of gene pools and mechanisms of genetic adaptation.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1607 - 1615"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145705988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HSPA1A, HSBPA1, AFAF, AQP3, and AQP11 Transcripts as Potential Markers of Sperm Quality in Holstein Bulls","authors":"O. Yu. Barkova,&nbsp;D. A. Starikova,&nbsp;I. V. Chistyakova,&nbsp;A. P. Dysin,&nbsp;N. V. Pleshanov","doi":"10.1134/S0003683825700048","DOIUrl":"10.1134/S0003683825700048","url":null,"abstract":"<p>Cryopreservation results in a significant reduction in the fertilizing properties of sperm, but genetic variations among different animal species affect the survival of germ cells after cryopreservation. Searching for specific genetic markers can be an effective way to select bulls before semen collection for cryopreservation. A correlation analysis of the expression level of candidate genes, <i>HSPA1A</i>, <i>HSBPA1</i>, <i>AFAF</i>, <i>AQP3,</i> and <i>AQP11</i>, with sperm quality indicators in native and decryopreserved sperm of Holstein bulls was carried out. Highly reliable positive correlations of the studied gene transcripts with a number of indicators of sperm viability and negative ones with indicators characterizing the loss of their functionality were revealed. The results of this study can be used to develop transcriptional biomarkers of bull sperm quality, namely based on the parameters of cryoresistance and the fertilizing potential of sperm.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1280 - 1286"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elastic Properties of Fibrous Nitrocellulose Matrices","authors":"R. M. Farzetdinova,&nbsp;L. M. Zaitov,&nbsp;T. D. Patsaev,&nbsp;V. V. Anokhin,&nbsp;E. Z. Meilikhov","doi":"10.1134/S0003683825700176","DOIUrl":"10.1134/S0003683825700176","url":null,"abstract":"<p>This work examines the mechanical properties of a transformable fibrous nitrocellulose matrix that can be used as a cell carrier in bioartificial cell systems. To measure the mechanical properties of fibrous matrices, a device was developed and constructed that can be used to conduct studies of a variety of matrices, which are the basis for populating cell cultures during the formation of artificial organs and tissues. The effect of reducing the mechanical stress and Young’s modulus of a wet nitrocellulose matrix compared to a dry one at the same relative deformation was discovered experimentally, which is due to the porosity of the matrix and fluid friction between the fibers. In the theoretical description of the mechanical properties, 2-, 3-, 5-, and 9-parametric Mooney–Rivlin hyperelastic models were used. Their parameters were calculated for both a dry and a wet nitrocellulose matrix based on the experimental data obtained, and a comparative analysis of the experimental data and theoretical dependences of mechanical stress and Young’s modulus were carried out. The results obtained can be used in the future for a detailed study of the cell-matrix interaction, which may make it possible to develop modern new generation biomaterials with physical properties close to living tissue for use in regenerative medicine.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1409 - 1422"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Beta-Galactosidase Activity of Yeast and Lactic Acid Bacteria after Separate and Co-Culture","authors":"M. A. Shpack,&nbsp;S. A. Ryabtseva,&nbsp;G. S. Anisimov,&nbsp;S. N. Sazanova,&nbsp;A. A. Semchenko,&nbsp;A. B. Chedenova","doi":"10.1134/S0003683825700061","DOIUrl":"10.1134/S0003683825700061","url":null,"abstract":"<p>The influence of mechanical and physical methods of cell disruption on the activity of beta-galactosidases produced by lactose-fermenting yeast and lactic acid bacteria after separate and co-cultivation in whey permeate was studied. It was found that ultrasound and heat treatment are the most effective methods that allow obtaining the enzyme with significantly higher activity than after quartz sand and glass bead mealing. The beta-galactosidases received as result of co-cultivation of some yeast strains and viscous strains of lactic acid bacteria had activity higher than beta-galactosidases from individual producers. The highest lactase activity (0.8–0.9 IU/ cm³) was obtained using heat treatment and the viscous strain of <i>Lactobacillus acidophilus</i> in combination with the yeasts <i>Kluyveromyces marxianus</i> Y-1338 and <i>Kluyveromyces lactis</i> Y-1333.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1296 - 1306"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fullerene C60 and Amino Acid Derivatives of Fullerene C60 as Biopreparations to Stimulate Winter Wheat Growth and Increase Plant Viability and Yields","authors":"O. V. Yamskova,&nbsp;V. S. Romanova,&nbsp;D. V. Kurilov,&nbsp;O. A. Shchuklina","doi":"10.1134/S0003683825700218","DOIUrl":"10.1134/S0003683825700218","url":null,"abstract":"<p>The proposed biotechnological approach is based on the use of aqueous dispersions or colloidal solutions of fullerene-containing compounds for seed material treatment, providing growth stimulation and helping to increase plant viability and productivity. The experiments showed the growth-stimulating effect on plants of winter wheat (<i>Triticum aestivum</i> L.) varieties “Zarya”, “Rubezhnaya”, and the trititrigia line (×<i>Trititrigia</i>) M116 of aqueous dispersions of C₆₀ fullerene and salts of amino acid derivatives of C₆₀ fullerene, used for presowing treatment (soaking) of seeds in a solution volume equivalent to their moisture capacity. Pre-sowing treatment (soaking) of winter wheat seeds of the “Rubezhnaya” variety in the presence of fullerene-containing aqueous dispersions in a field experiment demonstrated an increase in the yield and vitality of plants, and an increase in the weight and number of grains in an ear. The proposed biotechnological approach has prospects for effective use in agriculture for processing seed material, increasing plant viability and yield of important food crops.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1399 - 1408"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lyophylization of Produced Strains of the Rabies Virus","authors":"S. V. Generalov,&nbsp;A. V. Komissarov,&nbsp;E. G. Abramova,&nbsp;S. A. Badarin,&nbsp;N. M. Loginova,&nbsp;D. N. Bibikov,&nbsp;N. V. Sinitsina,&nbsp;E. A. Glazkova,&nbsp;G. N. Ginenko,&nbsp;A. K. Nikiforov","doi":"10.1134/S0003683825700401","DOIUrl":"10.1134/S0003683825700401","url":null,"abstract":"<p>The parameters of lyophilization of substances of organ-tissue and cultural origin containing a fixed rabies virus were determined. The objects of this research were the “Moscow 3253,” СVS, and “Saratov” strains of the rabies virus used in the production of commercial and experimental batches of rabies immunoglobulin from horse blood serum. For substances containing “Moscow 3253,” CVS, and “Saratov” strains, the effects of both freezing and eutectic temperatures were identical. The use of the developed lyophilization protocol allowed us to obtain samples as well-formed porous tablets. The infectivity of the freeze-dried Moscow 3253, CVS, and Saratov strains was 6.2, 7.1, and 6.8 logID₅₀/mL, respectively. Thus, the proposed freeze-drying regime is acceptable for obtaining samples of rabies virus strains used for the production of rabies immunoglobulin.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1616 - 1623"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145706042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Varietal Identification of Lupine (Lupinus L.) Based on Microsatellite DNA Markers","authors":"I. A. Klimenko,&nbsp;V. A. Dushkin,&nbsp;M. I. Lukashevitch,&nbsp;M. E. Selivanova,&nbsp;N. V. Novik,&nbsp;P. A. Ageeva","doi":"10.1134/S0003683825700231","DOIUrl":"10.1134/S0003683825700231","url":null,"abstract":"<p>Genetic polymorphism of a collection of accessions of the genus <i>Lupinus</i> was studied using SSR marking technology. The average level of intraspecies DNA polymorphism for <i>L. albus, L. angustifolius,</i> and <i>L. luteus</i> was found to be 25.8, 21.6, and 34.7%, respectively. The informative markers for genotyping and identification of Russian lupine varieties and breeding samples were determined. As a result of assessment of the genetic distance, genetically divergent accessions were identified that can serve as promising material for use in breeding programs for white (<i>L. albus</i>) and yellow (<i>L. luteus</i>) lupine, namely, SN 8-12, SN 96-15 DT1, KCI-22 d-15, KCI 22 d-17, 19-PIO-18-3, and SN 12-11-02-2-4-1. Based on the data obtained, molecular formulas of the studied samples were developed and reference genetic passports were compiled for the lupine varieties Belorozovyi 144, Nadyezhnyi, Antei, and Kurshavel. The practical results of this research and new knowledge on the structure of the genomes of Russian lupine varieties can be used in variety testing, seed production, and as a system of information support for the processes of selection and preservation of the national gene pool of lupine, a valuable agricultural crop.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 8","pages":"1477 - 1490"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145706105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of Components of the Aqueous Phase of the Process of Hydrothermal Liquefaction of Activated Sludge Biomass","authors":"S. V. Klementev,&nbsp;V. V. Lisun,&nbsp;Yu. V. Kulikova,&nbsp;A. S. Sirotkin","doi":"10.1134/S000368382570019X","DOIUrl":"10.1134/S000368382570019X","url":null,"abstract":"<p>Qualitative and quantitative characteristics of the aqueous phase obtained as a result of hydrothermal liquefaction of biomass of excessive activated sludge from municipal and domestic sewage treatment plants in Kaliningrad have been investigated experimentally. The content of organic compounds averaged 11 592 ± 571 mg O/dm³, and the biochemical index was 0.57. Aromatic compounds, carboxylic acids, and heterocyclic nitrogen-containing compounds were found in the composition of the aqueous phase. From activated sludge of treatment facilities of PJSC Nizhnekamskneftekhim, Republic of Tatarstan, 11 isolates–destructors of components of the aqueous phase were isolated. Their cultural and morphological characteristics were evaluated, and the necessary dilution of the aqueous phase for their cultivation was determined. It was noted that, in the process of cultivation of isolates, S2, S7, and S11 are able to neutralize components of the aqueous phase effectively in comparison with other isolated cultures: the efficiency of degradation by COD was 69, 73, and 75%, respectively. Based on 16S rRNA gene sequencing, isolate S2 had the highest similarity to <i>Pseudomonas glycinae</i> MS586 (99.52%), S7 with <i>Paenarthhobacter nicotinovorans</i> DSM 420 (99.8%), and isolate S11 with <i>Comamonas testosteroni</i> KS 0043 (99.6%). The most active isolates can be used for the treatment of highly concentrated wastewater before preliminary discharge into the sewerage network and natural water bodies.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 7","pages":"1443 - 1451"},"PeriodicalIF":1.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}