Applied Biochemistry and Microbiology最新文献_第9页

Isolation and Characterization of Biosurfactant-Producing Bacteria from Garlic Farmland Soil and Evaluation of Antimicrobial Activity 大蒜农田土壤中产生生物表面活性剂的细菌的分离、特征及抗菌活性评价

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-06-10 DOI: 10.1134/S0003683823603074

S. Ren, Y. Wu, Y. Wang, C. Yuan, Z. Liu, F. Zhao

{"title":"Isolation and Characterization of Biosurfactant-Producing Bacteria from Garlic Farmland Soil and Evaluation of Antimicrobial Activity","authors":"S. Ren, Y. Wu, Y. Wang, C. Yuan, Z. Liu, F. Zhao","doi":"10.1134/S0003683823603074","DOIUrl":"10.1134/S0003683823603074","url":null,"abstract":"Garlic suffers from frequent pathogenic diseases, seriously affecting its yield and quality. Biosurfactants have been reported with high antimicrobial activity and wide antimicrobial spectrum. Biosurfactant-producing bacteria existing in garlic farmland soil are expected to be used in control of garlic diseases. In this study, strain FA1 having high yield of biosurfactants was isolated from garlic farmland soil. Combined with morphological analysis and its 16S rDNA sequences analysis, the strain FA1 was identified as Pseudomonas aeruginosa. HPLC-MS analysis showed that biosurfactants produced from P. aeruginosa FA1 were presented rhamnolipids, mainly Rha-C8-C10, Rha-Rha-C8-C10, Rha-C10-C10 and Rha-Rha-C10-C10. The proportion of mono-rhamnolipids and di-rhamnolipids was about 1 : 1. P. aeruginosa FA1 can produce 7.96 g/L of rhamnolipids. The FA1 rhamnolipids can decrease the air-water surface tension to 27.5 mN/m with a critical micelle concentration of 50 mg/L. Through the agar diffusion method, P. aeruginosa FA1 exhibited high antimicrobial activity with diameters of inhibition zones larger than 20 mm to both the garlic potential pathogenic bacteria and fungi. Rhamnolipids produced from P. aeruginosa FA1 induced the potential pathogens to generate higher level of intracellular Reactive Oxygen Species (ROS), thus, exerting antimicrobial effects. P. aeruginosa FA1 can highly produce rhamnolipids biosurfactants and exhibit high antimicrobial effect to the garlic potential pathogens. This study verified that indigenous biosurfactant-producing bacteria from garlic farmland soil were feasible and effective to control garlic potential pathogens.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 4","pages":"640 - 648"},"PeriodicalIF":1.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141362539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Comprehensive Review of L-Asparaginase: Production, Applications and Therapeutic Potential in Cancer Treatment 全面回顾 L-天冬酰胺酶：癌症治疗中的生产、应用和治疗潜力

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-06-10 DOI: 10.1134/S0003683823602937

K. Hosseini, T. Zivari-Ghader, P. Akbarzadehlaleh, V. Ebrahimi, B. E. Sharafabad, A. Dilmaghani

引用次数: 0

Construction and Functional Screening of Metagenomic Libraries for D-Stereospecific Amidases and Their Catalytic Promiscuity 构建元基因组文库并对其进行功能筛选，以寻找 D-立体特异性酰胺酶及其催化杂合性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-06-10 DOI: 10.1134/S0003683823601889

S. Sinha

{"title":"Construction and Functional Screening of Metagenomic Libraries for D-Stereospecific Amidases and Their Catalytic Promiscuity","authors":"S. Sinha","doi":"10.1134/S0003683823601889","DOIUrl":"10.1134/S0003683823601889","url":null,"abstract":"Metagenomic fosmid libraries were prepared from soil and water samples collected from diverse places. Clones in the range of (1.1–2.8) × 103 were isolated from these samples and used for screening and isolation of D-amidase producer using dyes bromthymol green and phenol red. Maximum number of metagenomic libraries were obtained from soil rich in fishery waste while minimum number of libraries were obtained from tap water genome. Five positive clones after two-stage functional screening for D-alanine amidase were isolated on the basis of change in colour of growth medium due to enzyme production. Selected isolate on the basis of maximum enzyme production (2 U/mL) was cloned and overexpressed. Selected isolate from fishery waste metagenomes was found to be constitutive producer of D-amidase enzyme which after optimization led to 32% increase in enzyme activity. Isolated novel enzyme showed catalytic promiscuity with amides of D-Leu, D-Pro, D-Met and D-Val. Optimization of reaction conditions leading to production of 72% D-Ala and 65% D-Leu from their D-amide salts, respectively, in 8 h were confirmed by HPLC. Isolated homologous and putative genes after genomic screening were confirmed to be matching with Rhodococcus and Pseudomonas aeruginosa amidases. Enzyme also had acyl transferase activity with various amide substrates.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 4","pages":"614 - 626"},"PeriodicalIF":1.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141360908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of the Visual Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD) Method for Rapid Detection of Vibrio harveyi in Aquatic Animals 开发用于快速检测水生动物中 Harveyi 弧菌的可视环路介导等温扩增结合侧流浸量尺 (LAMP-LFD) 方法

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-06-10 DOI: 10.1134/S0003683824604062

S. H. Cai, Z. Chen, L. J. Sun, J. Zhong, Y. C. Huang, S. P. Yang, Y. S. Lu

{"title":"Development of the Visual Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD) Method for Rapid Detection of Vibrio harveyi in Aquatic Animals","authors":"S. H. Cai, Z. Chen, L. J. Sun, J. Zhong, Y. C. Huang, S. P. Yang, Y. S. Lu","doi":"10.1134/S0003683824604062","DOIUrl":"10.1134/S0003683824604062","url":null,"abstract":"Vibrio harveyi is a normal flora present in seawater, but in recent years, with large density aquaculture, V. harveyi has become one of the causative organisms in fish and shrimp. In this study, V. harveyi VieA gene was used as the target to design primers and establish the loop-mediated isothermal amplification (LAMP) combined with visual lateral flow test strip (LFD). The optimal reaction conditions were optimized as 60°C, 45 min, 1.2 mM Mg2+, 0.64 mM dNTPs, 0.25 mM betaine, and 16:1 ratio of internal/external primers. The results showed that the established LAMP assay was able to specifically detect V. harveyi with a sensitivity of 3.4 × 10–5 ng/µL. Clinical applicability analysis revealed that only V. harveyi was detected in the samples for pearl gentian grouper and whiteleg shrimp artificially infected by Vibrio. Therefore, we established a visual, reliable, rapid and sensitive LAMP-LFD method for detecting V. harveyi in aquatic animals.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 4","pages":"749 - 756"},"PeriodicalIF":1.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141364542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of Pseudomonas aeruginosa and Rhamnolipid for Bioremediation of Soil Contaminated with Waste Lubricant Oil 结合铜绿假单胞菌和鼠李糖脂对受废润滑油污染的土壤进行生物修复

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-06-10 DOI: 10.1134/S0003683823603232

P. R. M. Lopes, R. N. Montagnolli, G. Dilarri, C. R. Mendes, J. M. Cruz, M. P. Bergamini-Lopes, B. R. A. Moreira, J. Contiero, E. D. Bidoia

{"title":"Combination of Pseudomonas aeruginosa and Rhamnolipid for Bioremediation of Soil Contaminated with Waste Lubricant Oil","authors":"P. R. M. Lopes, R. N. Montagnolli, G. Dilarri, C. R. Mendes, J. M. Cruz, M. P. Bergamini-Lopes, B. R. A. Moreira, J. Contiero, E. D. Bidoia","doi":"10.1134/S0003683823603232","DOIUrl":"10.1134/S0003683823603232","url":null,"abstract":"Lubricant oils are largely responsible for environmental contamination and surfactants can hopefully improve the bioremediation process in soils contaminated with this kind of waste. However, to date only a few studies have elucidated how different surfactants affect the bioremediation process in oil-contaminated soils using the bioaugmentation technique. Thus, this study aimed to compare the effects of surfactants on the bioremediation process of waste lubricant oil in soil in association or not with bioaugmentation. Three biostimulating agents were tested: a chemical detergent, a synthetic surfactant, and a biosurfactant (rhamnolipid). The bioaugmentation process with Pseudomonas aeruginosa showed a faster biodegradation activity with total biodegradation on 181 day after application. The biosurfactant increased the number of microbial and fungal colonies leading to high biodegradation levels and a quicker treatment with or without the bioaugmentation. Bioaugmentation associated with biosurfactant reached 30.43 mg of CO2 of production on 181 day (90% of total biodegradation of waste lubricant oil) and reduced soil toxicity to 30%. Therefore, results showed that the association of biosurfactant and bioaugmentation with P. aeruginosa represents an ecologically viable strategy for bioremediation of soils contaminated with waste lubricant oil.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 4","pages":"627 - 639"},"PeriodicalIF":1.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of the Effectiveness of Biocatalytic Synthesis and Antibacterial Activity of Known Antibiotics and “Chimeric” Cephalosporin Compounds 生物催化合成的有效性以及已知抗生素和 "嵌合 "头孢菌素化合物抗菌活性的比较评估

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S000368382460355X

A. V. Sklyarenko, I. A. Groshkova, N. A. Gorbunov, A. V. Vasiliev, A. V. Kamaev, S. V. Yarotsky

{"title":"Comparative Evaluation of the Effectiveness of Biocatalytic Synthesis and Antibacterial Activity of Known Antibiotics and “Chimeric” Cephalosporin Compounds","authors":"A. V. Sklyarenko, I. A. Groshkova, N. A. Gorbunov, A. V. Vasiliev, A. V. Kamaev, S. V. Yarotsky","doi":"10.1134/S000368382460355X","DOIUrl":"10.1134/S000368382460355X","url":null,"abstract":"The processes of biocatalytic synthesis of cefamandole and cefazoline, as well as four “chimeric” cephalosporins carrying functional groups of these antibiotics in the C3 or C7 position of β-lactam, were carried out using immobilized cephalosporin-acid synthetase under mild standard conditions. A higher efficiency of biocatalytic acylation of β-lactams with a 1(H)-tetrazolylacetic acid residue was demonstrated compared to acylation with mandelic acid residue. The chemical structure of the obtained compounds was confirmed using the HPLC-MS method. The possibility of using directly reaction mixtures for evaluating the antibacterial activity of the compounds synthesized without isolating the target products is demonstrated. The activity of the obtained cephalosporins against twelve microorganisms belonging to the genera Enterococcus, Acinetobacter, Serratia, Pseudomonas, Staphylococcus, and Escherichia was evaluated by the method of diffusion into agar. The activity of synthesized “chimeric” cephalosporins against four microorganisms was found: Escherichia coli VKPM B-6695, Staphylococcus aureus VKPM B-6646, Staphylococcus aureus VKPM B‑8171, and Staphylococcus epidermidis VKPM B-12635.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"431 - 438"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140966717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Microplate Immunoenzyme Determination of Nonylphenol with Magnetic Sample Concentration 利用磁性样品浓度开发壬基酚微孔板免疫酶测定法

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603536

A. N. Berlina, L. V. Barshevskaya, K. V. Serebrennikova, N. S. Komova, A. V. Zherdev, B. B. Dzantiev

{"title":"Development of Microplate Immunoenzyme Determination of Nonylphenol with Magnetic Sample Concentration","authors":"A. N. Berlina, L. V. Barshevskaya, K. V. Serebrennikova, N. S. Komova, A. V. Zherdev, B. B. Dzantiev","doi":"10.1134/S0003683824603536","DOIUrl":"10.1134/S0003683824603536","url":null,"abstract":"Nonylphenol is an aromatic organic compound that has an estrogen-like effect and has a negative effect on the human endocrine system. A method has been developed for the competitive determination of nonylphenol using magnetic particles, rabbit antiserum, nonylphenol conjugate with soybean trypsin inhibitor (STI) and biotin. The principle of the analysis is the formation of immune complexes on the surface of magnetite particles due to covalent immobilization of protein G through the oriented immobilization of polyclonal antibodies from rabbit serum during a competitive reaction between the free analyte (nonylphenol) and the bound one (as part of the nonylphenol-STI-biotin conjugate) for the binding sites of specific antibodies. The detection of formed immune complexes is proposed to be carried out using a streptavidin-polyperoxidase conjugate, which makes it possible to achieve a nine-fold gain in the level of the analytical signal. The developed ELISA using magnetite particles allows us to achieve a detection limit of nonylphenol at the level of 3.8 ng/mL, which is 14.5 times lower in comparison with the classic competitive ELISA (55 ng/mL). Based on the results of the experimental work, the optimized volume of the test sample was 500 μL, which makes it possible to concentrate low-contaminated samples by 17 times.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"496 - 502"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140966520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Intensification of the Tricarboxylic Acid Cycle on Biosynthesis of Adipic Acid Through the Inverted Fatty Acid β-Oxidation by Escherichia coli Strains 强化三羧酸循环对大肠埃希菌菌株通过反式脂肪酸 β-氧化作用生物合成己二酸的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603573

A. Yu. Gulevich, A. Yu. Skorokhodova, V. G. Debabov

{"title":"Effect of Intensification of the Tricarboxylic Acid Cycle on Biosynthesis of Adipic Acid Through the Inverted Fatty Acid β-Oxidation by Escherichia coli Strains","authors":"A. Yu. Gulevich, A. Yu. Skorokhodova, V. G. Debabov","doi":"10.1134/S0003683824603573","DOIUrl":"10.1134/S0003683824603573","url":null,"abstract":"Using the previously engineered adipate-secreting Escherichia MG1655 lacIQ, ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDφ10-atoB, Ptrc-ideal-4-SDφ10-fadB, ∆fadE, PL-SDφ10-tesB, ∆yciA, Ptrc-ideal-4-SDφ10-fabI, PL-SDφ10-paaJ, ∆aceBAK, ∆glcB as the core strain, the derivatives capable of enhanced synthesis of the target compound from glucose via the reversed fatty acid β-oxidation pathway were obtained. The respective effect was achieved due to the intensification of the tricarboxylic acid cycle in the cells. Prevention of multiple cycle turnovers, resulting from the inactivation of succinate dehydrogenase, had no pronounced effect on the formation of adipic acid by the recombinant. Upon intensification of the cycle due to enhancing anaplerotic oxaloacetic acetic acid formation from phosphoenolpyruvate, resulting from the increased expression of the native ppc gene, the synthesis of adipic acid increased by 1.2-fold up to ~390 μM. Enabling the formation of oxaloacetate from pyruvic acid, by introducing heterologous Bacillus subtilis pyruvate carboxylase in the cells , resulted in a 1.5-fold intensification of the cycle, concomitantly with the proportional increase in adipic acid secretion to ~496 μM. Subsequent inactivation of sdhAB genes in the strain increased the secretion of the target compound only slightly, and the adipic acid titer reached ~520 μM. The obtained data indicated a direct dependence of the efficiency of adipic acid synthesis by the engineered strains on the degree of intensification of the tricarboxylic acid cycle.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"402 - 408"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-Acetylhomoserine Sulfhydrylase As a Key Enzyme of Direct Sulfhydrylation in Microbial Methionine Biosynthesis (A Review) O-乙酰高丝氨酸巯基酶是微生物蛋氨酸生物合成中直接巯基化的关键酶（综述）

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603561

V. V. Kulikova, E. A. Morozova, A. D. Lyfenko, V. S. Koval, N. V. Anufrieva, P. N. Solyev, S. V. Revtovich

{"title":"O-Acetylhomoserine Sulfhydrylase As a Key Enzyme of Direct Sulfhydrylation in Microbial Methionine Biosynthesis (A Review)","authors":"V. V. Kulikova, E. A. Morozova, A. D. Lyfenko, V. S. Koval, N. V. Anufrieva, P. N. Solyev, S. V. Revtovich","doi":"10.1134/S0003683824603561","DOIUrl":"10.1134/S0003683824603561","url":null,"abstract":"Methionine biosynthesis in most microorganisms proceeds in two alternative ways. Each pathway is catalyzed by independent enzymes and is tightly regulated by methionine. The transulfurylation pathway involves the formation of a cystathionine, and cysteine acts as a source of sulfur. The enzymes of this metabolic pathway are characterized in detail. The direct sulfhydrylation pathway involves the synthesis of homocysteine with the participation of an inorganic sulfur source directly from O-acetylhomoserine and is predominant in most classes of bacteria. The subject of this review is the properties and functioning of one of the least studied enzymes of the direct sulfhydrylation pathway—O-acetylhomoserine sulfhydrylase. A deep understanding of the mechanisms controlling the substrate and reaction specificity of O-acetylhomoserine sulfhydrylase is a necessary step in the rational redesign of the enzyme in order to create a promising catalyst for the synthesis of methionine and its derivatives, as well as, in combination with crystallographic data, for the development of new antimicrobial compounds based on effective enzyme inhibitors.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"359 - 371"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140971219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Conversion of Wood Materials from the Pulp and Paper Industry 纸浆和造纸工业木质材料的酶转化

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI: 10.1134/S0003683824603639

A. S. Aksenov, I. G. Sinelnikov, A. R. Shevchenko, K. A. Mayorova, D. G. Chukhchin, D. O. Osipov, M. V. Semenova, O. A. Sinitsyna, A. M. Rozhkova, E. V. Novozhilov, A. P. Sinitsyn

{"title":"Enzymatic Conversion of Wood Materials from the Pulp and Paper Industry","authors":"A. S. Aksenov, I. G. Sinelnikov, A. R. Shevchenko, K. A. Mayorova, D. G. Chukhchin, D. O. Osipov, M. V. Semenova, O. A. Sinitsyna, A. M. Rozhkova, E. V. Novozhilov, A. P. Sinitsyn","doi":"10.1134/S0003683824603639","DOIUrl":"10.1134/S0003683824603639","url":null,"abstract":"The reactivity during enzymatic hydrolysis of eight industrially produced samples of pulps and semichemical pulps by enzyme preparations of glycosyl hydrolases B151 and F10 produced by a strain of the ascomycete fungus Penicillium verruculosum has been determined. It is shown for the first time that among fibrous pulps available on the market of pulp and paper industry in Russia, the highest level of yield of glucose from the initial wood during biocatalysis using cellulases and hemicellulases is characteristic of semichemical pulps obtained after green liquor cooking of hardwood. A high degree of enzymatic conversion of softwood bleached kraft pulp has been established, which in combination with the possibility of obtaining modified polysaccharide materials from non-hydrolysable residue makes this cellulosic substrate the most promising for the development of biological processes at pulp and paper industries. It is shown that drying of pulp negatively affects the efficiency of cellulose hydrolysis, while mechanical refining improves the performance of the enzymatic saccharification process.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 3","pages":"448 - 456"},"PeriodicalIF":1.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140966842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0