二氯甲烷甲基杆菌的植物刺激活性。二氯甲烷DM4及其groEL2基因敲除突变体

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
N. V. Agafonova, G. A. Ekimova, Y. E. Firsova, M. L. Torgonskaya
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引用次数: 0

摘要

首次研究了二氯甲烷破坏者甲基细菌二氯甲烷亚种的基因组。分析了二氯甲烷DM4作为植物生长刺激剂的遗传决定因素,并确定了该菌株及其groEL2基因突变体促进植物生长的能力。菌株DM4的基因组包含参与植物激素(吲哚-3-乙酸和细胞分裂素)、铁载体、类胡萝卜素、聚β-羟基丁酸、水解酶以及参与d -半胱氨酸降解、紫外线损伤保护和磷酸盐溶解的酶的生物合成基因。在生菜生长试验中,接种菌株DM4对生菜幼苗的生长发育有积极的影响,提高了生菜幼苗对短期温度胁迫的适应性防御和抗性。对菌株DM4和菌株DM4 ΔgroEL2生长素、铁载体、水解酶、d -半胱氨酸脱氢酶活性和溶不溶性磷酸盐能力的比较分析表明,groEL2基因的破坏导致突变体吲哚衍生物的合成和溶磷酸盐能力下降。对这些菌株接种生菜植株的影响评估也表明,与原始菌株相比,DM4 ΔgroEL2的植物刺激潜能有所降低。结果表明,二氯甲烷芽孢杆菌中伴侣蛋白GroEL2的表达水平较低。二氯甲烷DM4间接影响其植物刺激活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Phytostimulating Activity of Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 and Its groEL2 Gene Knockout Mutant

Phytostimulating Activity of Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 and Its groEL2 Gene Knockout Mutant

For the first time, the genome of the dichloromethane destructor Methylobacterium dichloromethanicum subsp. dichloromethanicum DM4 was analyzed for the presence of genetic determinants indicating its potential as a plant growth stimulator, and the ability of this strain and its groEL2 gene mutant to improve plant growth was determined. The genome of strain DM4 contains genes involved in the biosynthesis of phytohormones (indolyl-3-acetic acid and cytokinins), siderophores, carotenoids, poly-β-hydroxybutyrate, hydrolytic enzymes, as well as enzymes involved in the degradation of D-cysteine, protection from UV-damage, and phosphate solubilization. Inoculation of lettuce sprouts by strain DM4 had a positive effect on plant growth and development and increased adaptive defense and resistance to short-term temperature stress in plant growth experiments. Comparative analysis of the production of auxins, siderophores, hydrolytic enzymes, D-cysteine desulfohydrase activity, and the ability to solubilize insoluble phosphates in strains DM4 and DM4 ΔgroEL2 showed that disruption of the groEL2 gene led to a decrease in the synthesis of indole derivatives and phosphate solubilizing ability in the mutant. Assessment of the impact of inoculation of lettuce plants by these strains also demonstrated a decrease in the phytostimulating potential of DM4 ΔgroEL2 compared to the original strain. The data obtained indicate that the chaperonin GroEL2 in M. dichloromethanicum subsp. dichloromethanicum DM4 indirectly affects its phytostimulating activity.

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来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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