N. P. Antonova, I. V. Grigoriev, A. M. Lendel, O. V. Usacheva, A. A. Klimova, E. V. Usachev, V. A. Gushchin, D. V. Vasina
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引用次数: 0
Abstract
The potential of new genetically modified recombinant endolysins as antimicrobial agents against Gram-negative bacteria was investigated. A series of enzymes based on LysSi3 lysozyme-like muramidase was obtained by modifying its sequence with antimicrobial peptides of different families, and recombinant expression in E. coli was demonstrated. Modification of LysSi3 resulted in increased bacteriolytic activity against the model isolate of A. baumannii and a higher kinetics rate compared to the native enzyme. The cytotoxic properties of the newly engineered lysins were investigated with the HEK293 and HaCaT cell lines, and it was shown that modification of LysSi3 with antimicrobial peptides does not significantly increase the toxic properties in vitro.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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