Applied Biochemistry and Microbiology最新文献_第2页

New Tools for Stable Highly Efficient Gene Expression in Rhodococcus spp. 稳定高效表达红球菌基因的新工具

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700248

A. O. Shemyakina, E. G. Grechishnikova, M. S. Potapova, O. V. Kozhaeva, L. E. Ryabchenko, A. S. Derunets, D. D. Derbikov, A. D. Novikov, K. V. Lavrov, A. S. Yanenko

{"title":"New Tools for Stable Highly Efficient Gene Expression in Rhodococcus spp.","authors":"A. O. Shemyakina, E. G. Grechishnikova, M. S. Potapova, O. V. Kozhaeva, L. E. Ryabchenko, A. S. Derunets, D. D. Derbikov, A. D. Novikov, K. V. Lavrov, A. S. Yanenko","doi":"10.1134/S0003683824700248","DOIUrl":"10.1134/S0003683824700248","url":null,"abstract":" A new Escherichia coli–Rhodococcus bireplicon plasmid pRY3-Rho-MCS, which is low-copy in Rhodococcus and multicopy in E. coli and stably inherited in Rhodococcus, was constructed on the basis of the previously used plasmid pRY16. The new plasmid is capable of conjugative transfer from E. coli to R. rhodochrous and R. qingshengii. The resulting R. rhodochrous and R. qingshengii strains with the new plasmid stably overproduced the green fluorescent protein turboGFP under the control of the corynebacterial Ptuf promoter. The efficiency of the target gene expression in the engineered strains was evaluated by flow cytofluorimetry and the possibility of using this technology in the search for highly active promoters for target gene expression in Rhodococcus was demonstrated. The instability of inheritance of the original plasmid pRY16 in R. rhodochrous cells is potentially suitable for the development of CRISPR/Cas-based methods for genome editing of Rhodococcus spp. It is concluded that the obtained tools, the plasmid pRY3-Rho-MCS and the promoter Ptuf, have a significant potential for application in biotechnology, including the development of biocatalysts, as well as producers of bacterial proteins that are difficult to synthesize in E. coli and other model microorganisms.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1638 - 1647"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of OPI1 Gene Inactivation on the Production of Heterologous Phytase and β-Amylase in Komagataella phaffii OPI1基因失活对法菲Komagataella异源植酸酶和β-淀粉酶产生的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824090035

A. A. Tkachenko, L. N. Borshchevskaya, S. P. Sineoky, T. L. Gordeeva

{"title":"Effect of OPI1 Gene Inactivation on the Production of Heterologous Phytase and β-Amylase in Komagataella phaffii","authors":"A. A. Tkachenko, L. N. Borshchevskaya, S. P. Sineoky, T. L. Gordeeva","doi":"10.1134/S0003683824090035","DOIUrl":"10.1134/S0003683824090035","url":null,"abstract":"A new recipient strain, Komagataella phaffii VKPM Y-5229 (Δhis4Δopi1), has been developed. Its use allows constructing strains with increased production of recombinant proteins. The OPI1 gene encoding the Opi1 transcription regulator, a functional analogue of the negative regulator of lipid biosynthesis Opi1p from Saccharomyces cerevisiae, was identified. The OPI1 gene was knocked out in the K. phaffii VKPM Y-5013 (Δhis4) strain using the highly efficient CRISPR/Cas9 genome editing system. The effect of OPI1 gene inactivation on the production of recombinant heterologous enzymes, Citrobacter gillenii phytase and Priestia flexa β-amylase, was studied. The average productivity of transformants based on the VKPM Y-5229 (Δhis4Δopi1) strain secreting phytase or β-amylase increased by 18 and 25%, respectively, compared to the productivity of transformants derived from the parent strain. Furthermore, it was demonstrated that inactivation of the OPI1 gene in a multicopy phytase-producing strain led to an increase in productivity by more than 26%. The results obtained can be employed for developing industrial strains producing heterologous proteins based on K. phaffii, as well as for increasing the production of targeted proteins in existing producing strains.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1658 - 1664"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospects for Obtaining L-Methionine Using Biotechnological Methods Based on Escherichia coli and Corynebacterium glutamicum: Part 1. Application, Methods of L-Methionine Production, and Regulation of its Biosynthesis in Bacteria 基于大肠杆菌和谷氨酸棒状杆菌的生物技术制备l -蛋氨酸的前景（一）。l -蛋氨酸的应用、生产方法及其在细菌中的生物合成调控

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700042

V. A. Livshits, D. M. Bubnov, T. E. Shustikova, A. A. Khozov, T. E. Leonova, L. E. Ryabchenko, T. V. Vybornaya, A. A. Stepanova, A. S. Yanenko

引用次数: 0

Taxonomic Affiliation and Fungicidal and Protective Activities of the Bacillus amyloliquefaciens 16-1111 Strain 解淀粉芽孢杆菌16-1111菌株的分类归属及其杀真菌和保护活性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700121

N. I. Kuznetsova, Yu. E. Kozlovsky

{"title":"Taxonomic Affiliation and Fungicidal and Protective Activities of the Bacillus amyloliquefaciens 16-1111 Strain","authors":"N. I. Kuznetsova, Yu. E. Kozlovsky","doi":"10.1134/S0003683824700121","DOIUrl":"10.1134/S0003683824700121","url":null,"abstract":" The experimental data on the study of the taxonomic position and biological properties, including fungicidal and protective activities, of a strain of spore-forming bacteria typified as Bacillus amyloliquefaciens based on the analysis of sequences of variable regions of the 16S rRNA gene are presented. The strain was established to have a wide spectrum of fungicidal activity and to suppress the development of representatives of six genera of phytopathogenic fungi that play a major role in the pathogenesis of agricultural crops, namely, Fusarium (F. solani, F. oxysporum, F. graminearum, F. nivale), Rhizoctonia (R. solani), Alternaria (A. tenuis), Phoma (P. solanicola), Sclerotinia (S. sclerotiorum), Botrytis (B. cinerea), and Magnaporthe (M. grisea). In vitro and in vivo experiments for the culture fluid of the B. amyloliquefaciens 16-1111 strain demonstrated, on the one hand, the absence of phytotoxicity and, on the other hand, protective and stimulating effects on germinating seeds of vegetable crops of the family Cucurbitaceae and plants cultivated in protected ground.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1526 - 1533"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active Inclusion Bodies Protect the Target Protein from Degradation in Escherichia coli Cells 活性包涵体保护大肠杆菌细胞中的靶蛋白不被降解

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700236

A. S. Komolov, E. S. Bobrov, E. P. Sannikova, I. I. Gubaidullin, N. O. Chernomorova, D. G. Kozlov

{"title":"Active Inclusion Bodies Protect the Target Protein from Degradation in Escherichia coli Cells","authors":"A. S. Komolov, E. S. Bobrov, E. P. Sannikova, I. I. Gubaidullin, N. O. Chernomorova, D. G. Kozlov","doi":"10.1134/S0003683824700236","DOIUrl":"10.1134/S0003683824700236","url":null,"abstract":" The production of target proteins in Escherichia coli cells can be greatly simplified if they are synthesized in a biologically active state as part of active inclusion bodies (AIBs), which can easily be isolated from cells by centrifugation. This is a new technology, so the question about the protective properties of AIBs specific for standard inclusion bodies still remains open. This work describes the synthesis of the recombinant protein L6KD-SUMO-[R34-GLP-1(7–37)], which forms AIB, in E. coli BL21(DE3) cells. This protein engineered from a novel, recently developed L6KD-SUMO platform incorporates a modified human glucagon-like peptide-1, R34-GLP-1(7–37), the active substance of Liraglutide-based drugs. It was shown that, the soluble protein His10-SUMO-[R34-GLP-1(7–37)] expressed by E. coli, retained the peptide intact only for 24 h, but the peptide integrity in the AIB composition was maintained over 70 h of cell cultivation. Thus, it is logical to assume that AIB formation has a protective effect on target compounds synthesized by the cell.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1631 - 1637"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Recombinant Vaccinia Virus Expressing a Sodium/Iodide Symporter for Theranostics of Malignant Tumors Using Synchrotron Radiation 表达钠/碘同体的重组痘苗病毒在同步辐射治疗恶性肿瘤中的应用

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700273

A. V. Semenova, A. A. Grazhdantseva, G. F. Sivolobova, G. A. Kudrov, A. S. Kabanov, A. A. Legkodymov, K. E. Kuper, A. P. Agafonov, G. V. Kochneva

{"title":"A Recombinant Vaccinia Virus Expressing a Sodium/Iodide Symporter for Theranostics of Malignant Tumors Using Synchrotron Radiation","authors":"A. V. Semenova, A. A. Grazhdantseva, G. F. Sivolobova, G. A. Kudrov, A. S. Kabanov, A. A. Legkodymov, K. E. Kuper, A. P. Agafonov, G. V. Kochneva","doi":"10.1134/S0003683824700273","DOIUrl":"10.1134/S0003683824700273","url":null,"abstract":" A recombinant strain of the vaccinia virus, VV-mNIS-dGF, has been constructed. The genome of this strain contains an insertion of the mouse sodium/iodide symporter transgene (mNIS). This insertion is located in the deleted region of the viral thymidine kinase gene (tk). In addition, the viral growth factor gene (vgf) was deleted, ensuring that the virus had an attenuated effect on normal cells without affecting its replication in cancer ones. The antitumor activity of the VV-mNIS-dGF strain in two mouse cancer models, melanoma B16-F10 and colorectal carcinoma CT-26, was studied. In both models, the strain exhibited high oncolytic activity, efficiently expressed the mNIS and accumulated iodide in tumor cells in both in vitro experiments and in vivo on mouse allografts. Quantification of the iodide content in tumors was performed by elemental analysis using X-ray Fluorescence Analysis with Synchrotron Radiation (XRF-SR). The results indicate the possibility of using the VV-mNIS-dGF strain for both therapy and diagnosis (theranostics) of malignant tumors.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1708 - 1717"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systems Metabolic Engineering of Corynebacterium glutamicum to Create a Producer of L-Valine 谷氨酸棒状杆菌产生l -缬氨酸的系统代谢工程

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824090023

M. E. Sheremetieva, T. M. Khlebodarova, D. D. Derbikov, V. V. Rozantseva, N. A. Kolchanov, A. S. Yanenko

{"title":"Systems Metabolic Engineering of Corynebacterium glutamicum to Create a Producer of L-Valine","authors":"M. E. Sheremetieva, T. M. Khlebodarova, D. D. Derbikov, V. V. Rozantseva, N. A. Kolchanov, A. S. Yanenko","doi":"10.1134/S0003683824090023","DOIUrl":"10.1134/S0003683824090023","url":null,"abstract":" This review is devoted to the main approaches of systems metabolic engineering used in the development of Corynebacterium glutamicum–based producers of L-valine, an essential proteinogenic amino acid that is widely used as a feed additive. This direction of engineering is based on the concepts of systems biology, considering the living organism as a holistic system with complex interaction between elements. This review considers the following approaches: laboratory evolution as a way to obtain new mutations that increase L-valine production; the use of biosensors to select clones with such mutations; “omic” data obtained for C. glutamicum strains capable of producing L-valine and related amino acids; modification of genes encoding global regulatory factors; and modeling of metabolic pathways relevant to amino acid production in C. glutamicum using full genomic data. Each of the approaches is evaluated in terms of its effectiveness in increasing the productivity of strains and improving their technological characteristics. In addition, information is presented on modern methods of genome editing of C. glutamicum, without which it is impossible to carry out a large number of targeted modifications necessary for the realization of systems metabolic engineering approaches.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1603 - 1622"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Yarrowia lipolytica Ribosomal Promoter pRPL25 as a Promising Object for Metabolic Engineering of Production Strains 多脂耶氏菌核糖体启动子pRPL25作为生产菌株代谢工程的有前景的对象

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700224

A. A. Cherenkova, B. V. Sviridov, T. K. Dvoryanchikova, O. E. Melkina

{"title":"The Yarrowia lipolytica Ribosomal Promoter pRPL25 as a Promising Object for Metabolic Engineering of Production Strains","authors":"A. A. Cherenkova, B. V. Sviridov, T. K. Dvoryanchikova, O. E. Melkina","doi":"10.1134/S0003683824700224","DOIUrl":"10.1134/S0003683824700224","url":null,"abstract":" Promoters are crucial components of yeast production strains in metabolic engineering. Due to its high activity, the promoter of the RPL25 gene, which encodes the L25 protein of the large ribosomal subunit, is a promising object for investigation and optimization. Here, to investigate the Yarrowia lipolytica pRPL25 promoter structure, a randomized library of its sequences was generated in 100 nucleotide increments. The strength of the obtained promoters was evaluated using the green fluorescent protein as a reporter. The results showed that the minimum functional length of the studied pRPL25 derivatives was 199 bp, and the optimal functional length was 497 bp. When the sequence was shortened to 400 bp, the expression level of the reporter decreased significantly. Based on the above, we propose that the 400–500 bp region of pRPL25 comprises an upstream activating sequence (UAS). The pRPL25 promoter and its derivatives potentially can be used in bioengineering to create metabolic pathways with a precise level of gene expression. Strains constructed on their basis may be more efficient producers of target compounds compared to classical expression systems such as Saccharomyces cerevisiae.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1623 - 1630"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and Function of Cytochrome with Cardiolipin: Enzymatic Activity of the Complex and Free-Radicals Processes 细胞色素与心磷脂的结构和功能：复合物和自由基过程的酶活性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700297

I. N. Levchenko, G. K. Vladimirov, I. V. Volodyaev, Y. A. Vladimirov

{"title":"Structure and Function of Cytochrome with Cardiolipin: Enzymatic Activity of the Complex and Free-Radicals Processes","authors":"I. N. Levchenko, G. K. Vladimirov, I. V. Volodyaev, Y. A. Vladimirov","doi":"10.1134/S0003683824700297","DOIUrl":"10.1134/S0003683824700297","url":null,"abstract":"The points of enzymatic activity, quantum yields, structure, and functions of luminol chemiluminescence activated by physical activators coumarinomics ({{C}_{{314}}}) and ({{C}_{{525}}}) under the action of (CytC-CL~)complex in aqueous medium and in a nonpolar environment have been modelled. It is shown that the enzymatic activity points and quantum yields are significantly higher in the presence of the physical activator coumarin ({{C}_{{525}}}) than in the case of its own non-activated luminescence and in the case of the physical activator ({{C}_{{314}}}). The enzymatic activity depends not only on the concentration of cytochrome C but also on the percentage ratio between its native and partially denatured forms. The release of the (CytC-CL~) complex into the cytoplasm leads to activation of membrane lipid peroxidation processes and a cascade of enzymatic reactions leading to apoptosis. Parameters investigated by kinetics of activated chemiluminescence, based on the data of cancer cells, confirm the properties to resist apoptosis and mutations, i.e. the reason for the appearance of different kinds of metastasis. The results of modelling are applicable to the analysis of natural apoptogens, which are the basis for the creation of drugs and lead to increased resistance of the organism to the effects of various factors.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1718 - 1722"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Industrially Significant Brevibacillus laterosporus Strain for the Production of Biogenic Silver-Containing Nanoparticles: Analysis of Physicochemical, Biological, and Antimicrobial Characteristics of Nanomaterial 具有工业意义的生产生物含银纳米颗粒的短芽孢杆菌菌株：纳米材料的理化、生物学和抗菌特性分析

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700078

T. A. Voeikova, O. A. Zhuravliova, N. I. Kuznetsova, T. D. Patsaev, A. L. Vasiliev, N. V. Bulushova, E. I. Kozhukhova, P. A. Zhdanov, E. S. Bobrov, V. G. Debabov

{"title":"The Industrially Significant Brevibacillus laterosporus Strain for the Production of Biogenic Silver-Containing Nanoparticles: Analysis of Physicochemical, Biological, and Antimicrobial Characteristics of Nanomaterial","authors":"T. A. Voeikova, O. A. Zhuravliova, N. I. Kuznetsova, T. D. Patsaev, A. L. Vasiliev, N. V. Bulushova, E. I. Kozhukhova, P. A. Zhdanov, E. S. Bobrov, V. G. Debabov","doi":"10.1134/S0003683824700078","DOIUrl":"10.1134/S0003683824700078","url":null,"abstract":"Abstract—The Brevibacillus laterosporus strain with a high level of bactericidal and fungicidal activity was used for the first time for the biosynthesis of silver-containing nanoparticles of various compositions. Nanomaterial samples were obtained by reduction of Ag+ from AgNO3 solution or by a chemical reaction between Na2S2O3 and AgNO3 in the presence of B. laterosporus cells. When assessing the chemical composition and crystal structure of the nanomaterial, it was found that the shape of the nanocrystals was close to spherical, and the particle sizes varied from 2 to 15 nm, with an average value of 5–7 nm. The values of the ζ-potentials of the samples were close and were –23…–25 mV. The dimensions of the hydrodynamic diameter indicated the presence of a bio-layer on the surface of nanocrystals of various types. The presence of proteins on the surface of nanoparticles was established by electrophoresis, and the selectivity of sorption of protein molecules of various molecular weights from the culture liquid of B. laterosporus on their surface was shown. Several proteins have been identified by mass spectroscopy. A difference in the level of biocidal activity of silver and silver sulfide nanoparticles formed in the presence of B. laterosporus cells was found in relation to gram(+) and gram(–) bacteria and yeast.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 7","pages":"1481 - 1492"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0