Applied Biochemistry and Microbiology最新文献

{"title":"Molecular Aspects of Pyrophosphate Regulation of the Activity of Isozymes of Maize Fructose-1,6-Bisphosphate Aldolase","authors":"D. N. Fedorin,&nbsp;A. E. Khomutova,&nbsp;D. V. Nikitenko","doi":"10.1134/S0003683826600569","DOIUrl":"10.1134/S0003683826600569","url":null,"abstract":"<p>Two isoenzymes of cytoplasmic aldolase from maize leaves were purified using ion-exchange chromatography. Using a linear NaCl gradient during elution from DEAE-Sephacel column allowed the separation of isoenzymes based on their surface charge. At the concentrations of 101.5 and 122.5 mM sodium chloride, the first and second forms of aldolase were desorbed from the column, respectively. Analysis of kinetic parameters, <i>K</i>_M and <i>V</i>_max, revealed differences in the affinity of aldolase isoenzymes for the substrate. In addition, these enzyme forms were regulated differently at different concentrations of pyrophosphate (PPi). Molecular docking allowed to analyze the type of bonds formed during the interaction of aldolase isoenzymes with pyrophosphate. The ALDO2 protein was characterized by the interaction of Glu144 with pyrophosphate via an additional hydrogen bond. Consequently, the effect on its catalytic function became more pronounced since it can participate in proton transfer during substrate cleavage. The NMR results showed a shift of 1.09 ppm, which may indicate a more significant change in the chemical environment due to the formation of PPi bonds with the amide or carboxyl groups of the amino acids of the active site of ALDO1. NMR and molecular docking results confirm that the aldolase isoenzymes ALDO1 and ALDO2 react differently with pyrophosphate, which is reflected in the regulation of their activity.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"768 - 776"},"PeriodicalIF":1.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148781970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Potential of Halotolerant Bacteria of the Genus Bacillus from Lake Solenoye (Lushnikovsky Reserve) and their Ability to Biosynthesize Surfactin","authors":"A. V. Chumak,&nbsp;O. V. Kisil,&nbsp;O. N. Sineva,&nbsp;M. B. Viryasov,&nbsp;M. V. Serebryakova,&nbsp;E. A. Kubareva,&nbsp;N. N. Markelova,&nbsp;V. S. Sadykova","doi":"10.1134/S0003683826600995","DOIUrl":"10.1134/S0003683826600995","url":null,"abstract":"<p>The species diversity and spectrum of antibiotic activity of 66 halotolerant bacilli isolated from the bottom sediments of the relict Lake Solenoe of the Lushnikovsky Nature Reserve of the Stavropol Territory were evaluated in relation to a number of phytopathogenic and conditionally pathogenic bacteria and fungi. The strains were represented by 8 species of the genus: <i>Bacillus megaterium, B. pumilus, B. mojavensis, B. atrophaeus, B. cereus, B. thuringiensis, B. marisflavi, B.licheniformis</i> and 4 strains belonged to the closely related taxa <i>Paenibacillus glucanolyticus, P. elgi</i>, <i>Cytobacillus oceanisediminis</i> and <i>C. firmus</i>. As a result of antimicrobial activity screening, 18 strains were selected that exhibit high or moderate (growth inhibition above 50%) antimicrobial activity against all phytopathogenic bacteria of the genus <i>Pectobacterium, Staphylococcus aureus</i> ATCC 6538 and <i>Escherichia coli</i> ATCC 25922. The ability to synthesize surfactins was evaluated, and strain differences were identified in different types of bacilli. As a result of the research, a potentially promising producer was selected: strains <i>B. mojavensis</i> 1sl and <i>B. pumilus</i> 37sl, with 126 and 134 mg/L of surfactin yields, respectively.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"690 - 697"},"PeriodicalIF":1.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148782040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crop Adaptation to Combined Abiotic Stresses: Mechanisms and Microbial Partners (Review)","authors":"O. Lastochkina,&nbsp;A. Avalbaev,&nbsp;A. Lubyanova,&nbsp;D. Maslennikova,&nbsp;M. Lobova","doi":"10.1134/S0003683825604810","DOIUrl":"10.1134/S0003683825604810","url":null,"abstract":"<p>Combined abiotic stresses (CAS) (drought, salinity, heat, heavy metal toxicity, etc.) pose a significant threat to global food security under climate change, as they induce complex and non-additive plant responses. The present comprehensive review, incorporates a meta-analysis of recent studies, reveals that these combined effects generate unique, often synergistic physiological outcomes that cannot be extrapolated from studies of individual stresses. This review systematizes current knowledge on the types of CAS, their interactions, and their impacts on plant physiological, molecular, and microbiological processes. Particular attention is given to how CAS alter the structure and function of rhizosphere and endosymbiotic microbiomes, and how plants actively recruit beneficial microorganisms through the “cry for help” mechanism. The potential of microbial bioinoculants as a sustainable strategy for enhancing plant tolerance to CAS was analyzed. Although direct evidence of microbial efficacy under CAS conditions remains limited, this review extrapolates from established mechanisms, such as improved photosynthesis, osmolyte production, enhanced antioxidant activity, and hormonal modulation, to propose how microbes may mitigate combined stresses. Our analysis highlights that the role of microbial communities and their application as bioinoculants is critically underexplored in CAS research. Finally, we address the translational gap between laboratory study and field application, advocating for the use of omics technologies to guide the rational design of synthetic microbial consortia tailored to real-world multi-stress conditions.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"743 - 767"},"PeriodicalIF":1.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148782061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriocins as Next Generation Antimicrobials: Insights into their Mechanisms and Applications","authors":"A. Kumar,&nbsp;A. Kishore,&nbsp;G. Kaur,&nbsp;P. S. Kaur,&nbsp;B. Prasad","doi":"10.1134/S000368382560472X","DOIUrl":"10.1134/S000368382560472X","url":null,"abstract":"<p>Bacteriocins are a unique group of antimicrobial peptides synthesized by ribosomes, heat sensitive and have the potential to inhibit or kill related bacterial groups but can also have broad spectrum against other microorganisms. Bacteriocins exert inhibitory effects through diverse mechanisms viz. creating a pore in bacterial membranes, receptor-meditated cytotoxicity, and enzymatic degradation of cellular components. Due to its antimicrobial potential, it has a significant role in preservation processes, inhibiting the development of food spoilage microbes, infective bacteria, and serves as an antibiotic agent against drug-resistant strains in traditional medicine. Bacteriocins are also used in farming and agriculture to control phytopathogens and promote plant growth. These compounds can be utilized in conjunction with antibiotics or as a substitute to reduce the problem of antimicrobial resistance. Bacteriocins can be exploited as next generation natural antibiotics and antimicrobial drugs to treat and prevent gastric ulcers by killing their causal microorganism. Currently, bacteriocin is also used in oral and vaginal infections.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 4","pages":"670 - 689"},"PeriodicalIF":1.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148782062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Stable Reagent Kit for Single-Phase Bioluminescent Immunoassay of Tick-Borne Encephalitis Virus","authors":"A. N. Kudryavtsev,&nbsp;L. A. Frank","doi":"10.1134/S0003683826600387","DOIUrl":"10.1134/S0003683826600387","url":null,"abstract":"<p>A single-phase, competitive bioluminescence immunoassay has been proposed for the rapid detection of ticks infected with the tick-borne encephalitis virus (TBEV). This method uses split NanoLuc luciferase complementation, which is driven by the formation of biospecific complexes of a genetically fused anti-TBEV miniantibody and an immunogenic fragment of the viral capsid protein E. The furimazin-dependent bioluminescence of this complex is 2.5% that of full-length NanoLuc and exhibits similar signal kinetics. The presence of TBEV-associated targets, which compete for binding to the miniantibody, causes the immunocomplex to break down, resulting in a decrease in the bioluminescence signal. This study yielded a lyophilized reagent sample containing fusion proteins, the furimazine substrate, and bovine serum albumin as a stabilizer. The suitability of the reagent for detecting TBEV in tick samples stored for three months at 5°C has been demonstrated. Testing only requires adding tick extract to the lyophilized reagent, eliminating the need for washing, prolonged incubation, or additional reagents. The analytical system includes lyophilized positive and negative control samples.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"599 - 603"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symbiotic Agriculture: Harnessing Plant–Biotic Interactions for Enhanced Crop Productivity (Review)","authors":"J. Kaneriya,&nbsp;V. Pattani,&nbsp;K. Joshi,&nbsp;G. Sanghvi","doi":"10.1134/S0003683825603336","DOIUrl":"10.1134/S0003683825603336","url":null,"abstract":"<p>Symbiotic agriculture represents a novel and sustainable approach to enhancing agricultural productivity by focusing on mutualistic relationships between plants and beneficial microorganisms such as bacteria, fungi, and mycorrhizae. These interactions—including parasitism, competition, commensalism, and mutualism – exert substantial effects on plant health under both nutrient-enriched and nutrient-deficient conditions. Beneficial microbes such as rhizobia, mycorrhizal fungi, and plant growth-promoting rhizobacteria (PGPR) contribute to nitrogen fixation, increase nutrient availability, and strengthen plant defence against pathogenic attacks. Incorporating biotic interactions not only promotes ecosystem sustainability and vitality but also improves crop growth. Furthermore, recent advances in genomics, multi-omics, CRISPR, and precision agriculture have enhanced the efficiency of symbiotic agriculture by enabling the customized synthesis of microbial consortia for targeted applications. The implementation of these innovations has paved the way for agricultural systems that are both climate-resilient and regenerative. Such systems improve soil fertility, foster biodiversity, and optimize resource utilization. This review underscores the importance of harnessing plant–biotic interactions to advance farming practices and ensure global food security. It highlights the natural capacities of plant-associated microorganisms and advocates for an interdisciplinary approach. Ultimately, the transition from input-intensive farming methods to biologically integrated agricultural systems aligned with ecological principles is essential for sustainable agriculture.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"553 - 568"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinase Polymerase Amplification Coupled with Membrane Immunochromatography or Enzyme-Linked Immunoassay for Detection of Pathogenic Bacteria Listeria monocytogenes","authors":"T. S. Serchenya,&nbsp;K. U. Akhremchuk,&nbsp;L. N. Valentovich,&nbsp;V. S. Lapina,&nbsp;T. N. Bakayeva,&nbsp;O. V. Sviridov","doi":"10.1134/S0003683825603841","DOIUrl":"10.1134/S0003683825603841","url":null,"abstract":"<p>Highly sensitive bioanalytical systems have been developed and assessed for the detection of the pathogenic bacteria <i>Listeria monocytogenes</i>, which contaminates food products and causes dangerous foodborne illnesses. These systems integrate recombinase polymerase amplification (<b>RPA</b>) with lateral flow assay (<b>LFA</b>) on test strips or enzyme-linked immunosorbent assay (<b>ELISA</b>) in microplates. The detection limit was 1 fg/µL of <i>L. monocytogenes</i> genomic DNA. The efficiency of DNA extraction was shown to influence the detection limit of the bacteria in the test systems. The <i>bsh</i> gene fragment that was chosen for DNA amplification had not been used for RPA before, and it was found to be specific for all pathogenic <i>L. monocytogenes</i>. Additionally, no cross-reactions with other foodborne pathogens were observed. The minimum assay time was achieved with the use of the test systems. The effectiveness of the test systems for identifying <i>L. monocytogenes</i> in food was validated by using milk samples. The RPA-LFA and RPA-ELISA successfully detected <i>L. monocytogenes</i> cells at concentrations as low as 8 CFU/mL. The detection limit can be reduced to 2 bacterial cells per 25 g of sample after enrichment for 5 h. The proposed test systems are a promising tool for monitoring the potential presence of <i>L. monocytogenes</i> in food products, as they are both sensitive and rapid.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"604 - 617"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of Remazol Brilliant Blue R Dye with a New Strain of Stutzerimonas stutzeri VITKSS2","authors":"K. Sakthishabarish,&nbsp;P. Mahesh,&nbsp;K. Kannabiran","doi":"10.1134/S0003683825603117","DOIUrl":"10.1134/S0003683825603117","url":null,"abstract":"<p>Textile industries are the second-largest polluting sector, discharging dyeing effluents directly into water bodies without proper treatment. This causes soil and environmental pollution, resulting in various health hazards to all living beings. This study aims to decolorize, degrade, and detoxify the Remazol Brilliant Blue R (RBBR) dye using bacteria isolated from dye-contaminated sites. An isolated potent strain was identified as <i>Stutzerimonas stutzeri</i> by 16S rRNA gene sequencing and named as <i>S. stutzeri</i> VITKSS2 strain. Maximum decolorization up to 99.0 and 95.7% of RBBR dye by VITKSS2 was identified in 100 and 500 mg/L dye concentrations, respectively. The FTIR spectra analysis shows the significant reduction of major functional groups, such as hydroxyl (O–H), carboxylic (C=C), amino (N-H), and sulfoxide (S=O) identified after microbial treatment. A reduction of 67% of bis(2-ethylhexyl) phthalate after VITKSS2 treatment was identified in GC-MS analysis. Similarly, other toxic compounds of RBBR dye were significantly reduced after microbial treatment. The phytotoxicity study conducted on <i>Zea mays, Pisum sativum L, Arachis hypogaea, Triticum,</i> and <i>Eleusine coracana</i> showed 82.4% reduction of total dye toxicity after VITKSS2 treatment. To enhance the dye degradation efficiency, the presence of azo reductase enzyme activity in RBBR dye decolorization was tested. Additionally, VITKSS2 also has the plant growth promoting rhizobacteria activity along with antagonistic activity against various pathogens. Further assessing the environmental toxicity, we can reuse the microbial-treated wastewater for various applications.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"514 - 525"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Acetyl-CoA Synthetase Overexpression on Cell Growth and Recombinant Protein Biosynthesis in Escherichia coli","authors":"D. A. Grechko,&nbsp;M. D. Bochkareva,&nbsp;A. V. Dvoretskaya,&nbsp;Z. R. Khasanshina,&nbsp;R. V. Drai","doi":"10.1134/S0003683825603968","DOIUrl":"10.1134/S0003683825603968","url":null,"abstract":"<p>In this study, genetically modified <i>Escherichia coli</i> strains FS60 and FS61 were constructed, engineered for overexpression of acetyl-CoA synthetase (ACS) under promoters of different strengths. Since ACS catalyzes the activation of acetate to acetyl-CoA, its overexpression was hypothesized to alter acetate accumulation and influence recombinant protein formation. Comparative cultivations of these strains in bioreactors were performed alongside the industrial reference strain <i>E. coli</i> BL21. The study aimed to evaluate the effect of ACS overexpression on the accumulation of the toxic secondary metabolite, acetate, and the productivity of the target recombinant protein. Significant reductions in acetate accumulation were observed in <i>E. coli</i> FS61: 66 and 73% at 3 and 4 h after induction, respectively, relative to that in <i>E. coli</i> BL21 during high-density fermentation. In contrast, <i>E. coli</i> FS60, expressing ACS under a stronger promoter, exhibited only 20 and 4% decreases in acetate accumulation at the same time points. These results may indicate that excessive ACS expression under a strong promoter imposes a metabolic burden, possibly disturbing energy flux and, in turn, promoting acetate synthesis rather than its utilization. By comparison, moderate expression driven by the Tac promoter appeared to reduce extracellular acetate accumulation more effectively. Reduced acetate accumulation correlated with improved recombinant protein productivity. ACS overexpression enhanced specific productivity of the target protein in <i>E. coli</i> FS60 by 28 and 40% at 3 and 4 h after induction, respectively, and by 59 and 51% in FS61. However, both genetically engineered strains demonstrated reduced biomass accumulation compared to <i>E. coli</i> BL21, limiting volumetric productivity. This effect may be attributed to the additional metabolic burden imposed by ACS overexpression, which decreases the growth rate of the engineered strains. In conclusion, ACS overexpression enhances cell-specific productivity, but requires further optimization to achieve volumetric productivity comparable to the industrial <i>E. coli</i> BL21 strain. This approach holds promise for industrial biotechnology applications although cultivation conditions require refinement to mitigate metabolic burden and maximize production efficiency.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"453 - 460"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disposal of Extruded Polystyrene Waste using Beetles Ulomoides dermestoides and Analysis of Microbial Communities Associated with Their Gut","authors":"L. I. Shirinkina,&nbsp;Yu. V. Taktarova,&nbsp;A. I. Bastrakov,&nbsp;N. A. Ushakova,&nbsp;I. B. Kotova","doi":"10.1134/S0003683826600284","DOIUrl":"10.1134/S0003683826600284","url":null,"abstract":"<p>The ability of <i>Ulomoides dermestoides</i> beetles to utilize polystyrene foam (PS) waste was studied. At the initial beetle biomass to PS mass ratio of 10 : 1, the weekly loss in PS waste mass reached 11.11 ± 3.75% in the case of using only PS waste as feed and 19.73 ± 4.16% when bran was added to the feed along with PS. In the intestinal microbiota of beetles in vivo, the presence of PS waste led to an increase in the proportion of bacteria of the genera <i>Hafnia-Obesumbacterium</i>, <i>Klebsiella</i>, <i>Pseudomonas</i>. Cultivation of microbial communities from the beetle intestine in vitro under anaerobic conditions on a mineral medium in the presence of PS waste leads to an increase in microbial diversity. The microbial community isolated from the guts of beetles fed exclusively PS waste and subsequently cultured in PS-supplemented liquid medium showed the highest protein content (up to 40 mg/mL), the highest production of volatile fatty acids (formate, acetate, propionate, valeriate) and the highest production of carbon dioxide (up to 8.3 mM). After culturing the intestinal microbial community under anaerobic conditions in the presence of PS waste, bacteria of the genera <i>Anaerostignum</i>, <i>Sebaldella</i>, <i>Pseudomonas</i>, <i>Enterobacter</i> were found among the dominant groups.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"467 - 480"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683826600284.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}