Applied Biochemistry and Microbiology最新文献_第2页

Halotolerant Streptomyces albidoflavus INA 01478 as a Producer of Daidzein: Genome Annotation, purification and Antifungal Activity 耐盐白黄链霉菌INA 01478作为大豆苷元的生产者：基因组注释、纯化和抗真菌活性

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683825601465

O. N. Sineva, N. N. Markelova, K. V. Malysheva, O. A. Kolpakova, G. Kh. Kudryakova, I. A. Prokhorenko, O. V. Kisil, A. Yu. Simonov, V. I. Polshakov, I. B. Levshin, V. S. Sadykova

引用次数: 0

In Vitro Efficacy of Nisin Peptide on Biofilm Formation and Cell Invasion of Listeria monocytogenes Nisin肽对单核增生李斯特菌生物膜形成和细胞侵袭的体外影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683825601374

S. Babakhani, F. Hosseini, P. Pakzad, M. B. Torbati

{"title":"In Vitro Efficacy of Nisin Peptide on Biofilm Formation and Cell Invasion of Listeria monocytogenes","authors":"S. Babakhani, F. Hosseini, P. Pakzad, M. B. Torbati","doi":"10.1134/S0003683825601374","DOIUrl":"10.1134/S0003683825601374","url":null,"abstract":"This study investigated the effects of nisin concentrations on the biofilm formation and cellular invasion of Listeria monocytogenes. The study also examined the toxicity of nisin concentrations on human cell lines. Nisin is a lantibiotic with antimicrobial properties. L. monocytogenes is a deadly foodborne pathogen leading to listeriosis. Biofilm formation, cell invasion, toxin-antitoxin systems, and extracellular proteins are the primary virulence mechanisms of L. monocytogenes. The PCR method was used to confirm the presence of virulence genes in L. monocytogenes. A microplate assay was used for biofilm quantification. The effect of nisin on cell invasion and cytotoxicity in the HT-29 human cell line was investigated using invasion assay and thiazolyl blue tetrazolium bromide (MTT) methods, respectively. Nisin solutions of 104, 103, and 102 IU/mL inhibited biofilm formation by 56, 90, and 99%, respectively (p < 0.0001). Nisin at concentration of 102 IU/mL reduced the expression of biofilm formation genes: 2.5-fold for mazF, 3.9-fold for flaA, and 3.4-fold for cheA. However, the increased iap gene expression (p < 0.0001) observed at nisin of 102 IU/mL. Nisin solutions of 104, 103, and 102 IU/mL reduced the invasion into HT-29 cells by 42, 73, and 99%, respectively. Survival of the HT-29 cells treated with nisin solutions of 104, 103, and 102 IU/mL decreased by 2, 7, and 18%, respectively. The action of nisin can be enhanced through various innovative techniques, increasing its effectiveness against L. monocytogenes. Nisin significantly inhibited the expression of genes involved in biofilm formation, cell invasion, and cellular signal transduction in L. monocytogenes.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"889 - 897"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secondary Metabolite Analysis of Fusarium solani F1C1, an Endophytic Fungus from Dichrocephala integrifolia 植物内生真菌番茄镰刀菌F1C1次生代谢物分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683824606516

K. S. Nongthombam

{"title":"Secondary Metabolite Analysis of Fusarium solani F1C1, an Endophytic Fungus from Dichrocephala integrifolia","authors":"K. S. Nongthombam","doi":"10.1134/S0003683824606516","DOIUrl":"10.1134/S0003683824606516","url":null,"abstract":"Medicinal plants not only store bioactive compounds but also diverse species of fungal endophytes. This group of fungi produces secondary metabolites in large amount which possess significant biological activities. The present study involved the isolation of the endophytic fungus, Fusarium solani F1C1, from the inflorescence of Dichrocephala integrifolia. The identity was confirmed using ITS-rDNA gene sequencing. Secondary metabolites were collected from F. solani F1C1 and extracted using ethyl acetate. Quantitatively, total flavonoid content of 10.21 (µg of quercetin equivalent/mg of endophyte extract) and total phenolic content of 30.47 (µg of gallic acid equivalent/mg of endophyte extract) were also recorded. The crude extract was analysed using LC-MS to detect the presence of non-volatile compounds, and the most abundant compounds were tentatively identified as aspalathin and kaempferol. Antibacterial activity of ethyl acetate fraction revealed inhibition of the growth of two Gram-positive and two Gram-negative bacteria the with a maximum MIC against Bacillus cereus of 1.56 µg/mL. Further, analysis such as isolation of compounds and evaluation of their bioactive potential, are required to assess their potential for applications in pharmaceutical industries, medicine, and chemical-free agriculture.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"946 - 953"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Characterization of a Thermo-tolerant Endoglucanase from Fibrobacter succinogenes 琥珀酸原纤维杆菌耐热内切葡聚糖酶的表达与特性研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S000368382560109X

X. Wang, X. Yang, S. Kan, X. Liu, J. Sun, D. Wang, H. Li

{"title":"Expression and Characterization of a Thermo-tolerant Endoglucanase from Fibrobacter succinogenes","authors":"X. Wang, X. Yang, S. Kan, X. Liu, J. Sun, D. Wang, H. Li","doi":"10.1134/S000368382560109X","DOIUrl":"10.1134/S000368382560109X","url":null,"abstract":"Fibrobacter succinogenes, a Gram-negative bacterium in the phylum Fibrobacteres, has the ability to efficiently degrade cellulose by employing a new mechanism. Genomic analysis suggested F. succinogenes lacks exoglucanases and most of its cellulases lack recognizable carbohydrate-binding modules (CBMs). To explore the specificity of cellulase, Fisuc_1219, a typical GH8 endoglucanase, was expressed and characterized. Bioinformatics results showed that this enzyme possessed the (a/a)6 barrel that consists of 6 pairs of antiparallel a-helices. Fisuc_1219 was expressed in Escherichia coli and exhibited optimal activity at 40°C and pH 6.0 using carboxymethyl cellulose (CMC) as the substrate. This enzyme demonstrated stability under alkaline conditions, retaining approximately 80% activity at pH 8.0 and 9.0, and 50% activity at pH 10.0. It also displayed significant thermostability, maintaining 54% activity after 32 h at 70°C and 50% activity after 4 h at 80°C. The degradation products of CMC and regenerated amorphous cellulose included cellohexaose, cellopentaose, cellotetraose, cellotriose, cellobiose, and glucose, with cellotriose being identified as the predominant hydrolysis product. Among various metal ions, Mg2+ exhibited the strongest inhibitory effect on Fisuc_1219 activity, while chemical additives also showed varying degrees of inhibition. The characterization of Fisuc_1219 will provide theoretical guidance for clarifying the mechanism of cellulose degradation of F. succinogenes.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"856 - 864"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Activity Analysis of Eukaryotic Cell Lines in the Evaluation of Plant Extracts 植物提取物评价中真核细胞系动态活性分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683825601441

T. Zhilkina, I. Gerasimova, I. Yakovleva, S. Gaidukova, A. Zubritskiy, A. Kamionskaya

{"title":"Dynamic Activity Analysis of Eukaryotic Cell Lines in the Evaluation of Plant Extracts","authors":"T. Zhilkina, I. Gerasimova, I. Yakovleva, S. Gaidukova, A. Zubritskiy, A. Kamionskaya","doi":"10.1134/S0003683825601441","DOIUrl":"10.1134/S0003683825601441","url":null,"abstract":"Plants are an inexhaustible source of new biologically active compounds that can be used to fight against various human diseases, including cancer. This study evaluates the effects of Pontederia crassipes leaf/petiole extracts on HDF and HEK293 cell lines using an integrated approach combining live-cell imaging (Celena X cell imager) and resazurin-based viability assays. Methanolic extracts were prepared via two methods: (A) prolonged extraction and (B) ultrasonication. Dynamic changes in cell count and surface area were quantified through time-lapse imaging over 24 h, with trendline slope analysis (regression coefficients) providing growth kinetics. Ethanolic extracts (Method A) were later included for comparative analysis. Additionally, methanolic extract was fractionated by HPLC, and GC-MS revealed a fairly wide range of phytochemical components in methanol extract fractions. Methanolic extracts (Method A) showed dose-dependent effects, with HEK293 cells more sensitive (proliferation inhibition at 12.5 μg/mL) than HDF (200 μg/mL). Ethanolic extracts inhibited HDF at 25–200 μg/mL and HEK293 at 12.5 μg/mL. The developed method using live cell imaging showed that the decrease in resazurin signals correlated with cytostasis (stable cell count) rather than cytotoxicity. The results highlight the importance of using this method in conjunction with endpoint assays to obtain more detailed information when analyzing bioactive compounds.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"973 - 986"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Inulinases and Ethanol by Hanseniaspora uvarum from the Juice of Agave salmiana Leaves 用龙舌兰叶汁生产菊粉酶和乙醇的研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S000368382560112X

J. Silva-Mendoza, J. A. Gómez-Treviño, L. Chávez-Guerrero, M. N. Sánchez-González, M. E. Cantú-Cárdenas

{"title":"Production of Inulinases and Ethanol by Hanseniaspora uvarum from the Juice of Agave salmiana Leaves","authors":"J. Silva-Mendoza, J. A. Gómez-Treviño, L. Chávez-Guerrero, M. N. Sánchez-González, M. E. Cantú-Cárdenas","doi":"10.1134/S000368382560112X","DOIUrl":"10.1134/S000368382560112X","url":null,"abstract":"The increasing demand for renewable energy and sustainable enzymatic production has driven research into alternative microbial sources for bioethanol and enzyme production. This study evaluates the potential of Hanseniaspora uvarum, a non-Saccharomyces yeast, for the simultaneous production of inulinases and bioethanol using Agave salmiana leaf juice as a substrate. H. uvarum exhibited high inulinase activity (180.5 U/mL), efficiently hydrolyzing inulin into fermentable sugars. Additionally, it demonstrated high ethanol production, reaching 30.4 g/L with 74.5% yield, surpassing commercial Saccharomyces cerevisiae strains, which failed to grow in this medium. The key discovery was that H. uvarum can grow in agave juice, potentially due to its tolerance to inhibitory compounds, possibly including saponins, which inhibited the growth of commercial yeasts. These results highlight the biotechnological potential of H. uvarum for bioethanol production from the Agave biomass and emphasize the relevance of non-Saccharomyces yeasts in industrial fermentation processes. Future research should focus on optimizing fermentation conditions and scaling up the process for industrial applications.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"882 - 888"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategic Optimisation of Growth and Production of Bioactive Compounds in Nigrospora oryzae, an Endophytic Fungus Associated with Dioscorea bulbifera L. 黄薯蓣内生真菌米黑孢菌生长及活性物质生产的优化研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683824607005

S. Sharma, V. Sharma, M. K. Dhar, S. Kaul

{"title":"Strategic Optimisation of Growth and Production of Bioactive Compounds in Nigrospora oryzae, an Endophytic Fungus Associated with Dioscorea bulbifera L.","authors":"S. Sharma, V. Sharma, M. K. Dhar, S. Kaul","doi":"10.1134/S0003683824607005","DOIUrl":"10.1134/S0003683824607005","url":null,"abstract":"An endophytic fungus Nigrospora oryzae was isolated from Dioscorea bulbifera L., an important ethnomedicinal plant. A huge repertoire of natural products produced by the endophytic fungus exhibits various biological activities. The current study aimed at the optimisation of process parameters for the growth of N. oryzae and production of secondary metabolites. The optimized process parameters were used for the fermentation and production of secondary metabolites. It was followed by the isolation, purification, and characterization of structurally diverse and biologically significant compounds from the fermented extract of endophytic fungus, N. oryzae and assessment of their bioactive potential. Herein, we described the yield of two anthraquinone derivatives, nigrosporin B (1) and acetoxybostrycin (2) based on the spectral data from the pure fractions F1 and F2. Anthraquinones and their derivatives have drawn widespread attention as potential drug candidates. The compounds (1) and (2) exhibited strong inhibition potential against Staphylococcus aureus, Bacillus cereus and Escherichia coli. The compounds (1) and (2) demonstrated significant results for α-glucosidase inhibitory activity. Further, GC-MS analysis of the two fractions, F3 and F4, revealed the presence of specific fatty acids, including nervonic, oleic, linoleic, erucic, palmitic, caprylic, and stearic acids that exhibited α-glucosidase inhibitory activity. The experimental data showed that these endophytic secondary metabolites open up new perspectives for their usage as substantial targets for therapeutic drug discovery and management of diseases.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"954 - 966"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, Characterization, and Byproducts Analysis of Six Oil-Degrading Strains for Rural Food Waste Treatment 6株农村食物垃圾油降解菌的分离、鉴定及副产物分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683825600149

Y. Zhang, Y. Shen, J. Ding, H. Zhou, Y. Qin, H. Zhao, P. Xu, J. Wang, Q. Cheng, D. Zhang, S. Ma, P. Zhang, M. Yu

{"title":"Isolation, Characterization, and Byproducts Analysis of Six Oil-Degrading Strains for Rural Food Waste Treatment","authors":"Y. Zhang, Y. Shen, J. Ding, H. Zhou, Y. Qin, H. Zhao, P. Xu, J. Wang, Q. Cheng, D. Zhang, S. Ma, P. Zhang, M. Yu","doi":"10.1134/S0003683825600149","DOIUrl":"10.1134/S0003683825600149","url":null,"abstract":"The composting of food waste (FW) in rural areas can be enhanced by utilizing effective oil-removing strains. This study investigates the screening process, oil removal performance, and byproducts of efficient soybean oil-degrading microbial strains. The initial strain sources were collected during the high-temperature phase of aerobic FW composting. After six stages of gradient domestication with oil concentrations ranging from 1.6 to 16 g/L, the oil removal efficiency (RE) of the mixed strains exceeded 90% within 4 days. Through gradient dilution and separation, three fungi and three bacteria with effective oil-removing capabilities were identified. At an oil concentration of 10 g/L, the oil RE of four strains (Simplicillium cylindrosporum, Fusarium proliferatum, Agrobacterium tumefaciens, and Pseudomonas geniculata) exceeded 93% within 6 days. Notably, Pseudomonas geniculata achieved the high 95% oil RE within 4 days, demonstrating the highest efficiency among the six strains tested. Additionally, F. proliferatum and Luteibacter rhizovicinus exhibited the strongest surfactant production capabilities among the fungi and bacteria, respectively. The byproducts of the degradation process were analyzed in detail using gas chromatography for the first time, revealing six types of volatile fatty acids. Both propionic acid and n-valeric acid were completely degraded by all six strains, while acetic acid requires careful management to prevent environmental pollution. Except for acetic acid, A. tumefaciens was able to completely degrade the other five acids, making it an environmentally friendly option. The strains identified in this study hold significant potential for improving processes such as rural FW composting, oil-containing wastewater treatment, and other oil-containing waste management.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"923 - 933"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Insights of Mucin Foraging Enterococcus durans G322S from Healthy Human Feces 健康人类粪便中黏素觅食杜兰肠球菌G322S的功能研究

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S0003683825601039

G. Deswal, M. Selwal, K. K. Selwal

{"title":"Functional Insights of Mucin Foraging Enterococcus durans G322S from Healthy Human Feces","authors":"G. Deswal, M. Selwal, K. K. Selwal","doi":"10.1134/S0003683825601039","DOIUrl":"10.1134/S0003683825601039","url":null,"abstract":"The human gastrointestinal tract is a home to several novel, diverse and complex microbial species that maintain a commensal relationship with the host. In this study, a Gram-positive, cocci-shaped mucin-degrading bacterium was isolated from human feces and identified as Enterococcus durans G322S, which can utilize mucin as the sole carbon source. The qualitative data in the form of a halo around the colony confirmed the mucolytic activity and quantitative degradation evident 70 ± 2.1% decrease in oligosaccharide content and 35 ± 2.9% protein content. The extracellular glycosidase activities data further supported the notion regarding the involvement of enzymes in degradation of mucin glycans. Notably, this study moves beyond conventional mucin degradation isolation by functionally characterizing a safe, mucus-adapted strain with promising probiotic attributes. The G322S strain exhibited no virulence traits, i.e., hemolysis, gelatinase, biofilm, and exopolysaccharide production (EPS). It survived well under simulated gastrointestinal conditions and exhibited survivability at low pH (40–55%) and high bile salt concentrations (≥ 60% cell viability). Moreover, the strain showed considerable in vitro binding to mucin (~15%) and low antibiotic resistance rate (16.3%). It showed antagonistic activity ranging from 12–19 mm against Staphylococcus lentus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. These integrated functional traits such as mucin foraging, enzymatic profile, antimicrobial activity, and safety, collectively suggest E. durans G322S as a promising next-generation probiotic candidate for mucus niche. Further work is needed to unearth the molecular mechanisms underpinning the degradation of mucin oligosaccharides in the human gut and their consequences on the microbial flora.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"898 - 907"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression, Purification, Crystallization, and PAN-Cancer Analysis of the Human RNA 3'-terminal Phosphate Cyclase RTCA 人RNA 3′端磷酸环化酶RTCA的表达、纯化、结晶及泛癌分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-08-17 DOI: 10.1134/S000368382560006X

S. Duan, Y. Yuan, Z. Wei, X. Cai, Y. Wu

{"title":"Expression, Purification, Crystallization, and PAN-Cancer Analysis of the Human RNA 3'-terminal Phosphate Cyclase RTCA","authors":"S. Duan, Y. Yuan, Z. Wei, X. Cai, Y. Wu","doi":"10.1134/S000368382560006X","DOIUrl":"10.1134/S000368382560006X","url":null,"abstract":"RNA 3'-terminal phosphate cyclase (RTCA) is an enzyme that converts RNA 3'-terminal phosphate into an RNA 2',3'-cyclic phosphate terminus. RTCA regulates axon regeneration through the RNA repair and splicing pathway, as well as the production of ribosomal RNA and ribosome biogenesis. Although numerous biochemical studies have illuminated the function of human RTCA, the structure and how RTCA functions in various cancers remain to be elucidated. Here we expressed, purified, crystallized, and performed pan-cancer analysis of human RTCA. Combininig Ni-affinity chromatography with gel filter chromatography, high-purity RTCA protein was obtained. RTCA was crystallized utilizing the hanging drop vapor diffusion method. By preliminary X-ray diffraction analysis, it was shown that the resolution of the crystal was about 5 Å. In situ proteolytic analysis indicated that RTCA formed a compact structure in solution. Furthermore, our pan-cancer analysis revealed that RTCA is involved in the occurrence, poor prognosis, and development of various tumors, especially in lung cancer. Our pan-cancer analysis revealed that RTCA might act as a prognostic marker in lung cancer, and protein crystallization conditions provide a basis for RTCA protein structure elucidation.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 5","pages":"834 - 843"},"PeriodicalIF":1.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0