Applied Biochemistry and Microbiology最新文献_第2页

Peroxidase of Trametes hirsuta LE-BIN 072: Purification, Characteristics, and Application for Dye Decolorization

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605730

O. S. Savinova, T. V. Fedorova

引用次数: 0

Screening of Inhibitory Activity of Narrow Fractions of Humic Substances towards Different Types of Class A β-Lactamases

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605791

V. G. Grigorenko, M. Yu. Rubtsova, I. P. Andreeva, G. V. Presnova, T. A. Mikhnevich, S. Yiming, D. S. Kravtsova, A. I. Konstantinov, D. A. Arutyunyan, I. V. Perminova

{"title":"Screening of Inhibitory Activity of Narrow Fractions of Humic Substances towards Different Types of Class A β-Lactamases","authors":"V. G. Grigorenko, M. Yu. Rubtsova, I. P. Andreeva, G. V. Presnova, T. A. Mikhnevich, S. Yiming, D. S. Kravtsova, A. I. Konstantinov, D. A. Arutyunyan, I. V. Perminova","doi":"10.1134/S0003683824605791","DOIUrl":"10.1134/S0003683824605791","url":null,"abstract":"Inhibition of β-lactamases involved in the development of bacterial resistance to β-lactam antibiotics is promising for restoring their effectiveness as antibacterial drugs. In this work, we screened the inhibitory activity against some serine β-lactamases of class A among humic substances of various origins, which represent complex organic matrices, as well as their narrow fractions obtained by separating preparations by acidity and polarity. The structural and group composition of broad fractions was characterized by 13C NMR spectroscopy; the molecular composition of narrow fractions was characterized by Fourier transform-ion cyclotron resonance mass spectrometry. The widest specificity of inhibition against various ESBL (TEM-12, TEM-17, TEM-18) and the inhibitor-resistant β-lactamase TEM-34 was possessed by the fraction of humic acids of brown coal CHA-I, isolated at pH 9: the values of residual activity of TEM -12, TEM-17, TEM-18, and TEM-34 were (%) 52 ± 5, 52 ± 5, 40 ± 4, and 58 ± 6, respectively. The data obtained for the most active narrow fraction turned out to be comparable to the effectiveness of sulfohydantoins, a promising new class of inhibitors. This indicates the feasibility of further work on the fine fractionation of natural humic substances using 2D chromatography, which will open a new, extensive source of β-lactamase inhibitors of non-β-lactam nature.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1196 - 1208"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of DNA-binding Proteins on Terminal Deoxynucleotidyl Transferase Activity in Systems with Homopolymer Substrates

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605237

A. B. Sachanka, V. V. Shchur, S. A. Usanov, A. V. Yantsevich

{"title":"Effect of DNA-binding Proteins on Terminal Deoxynucleotidyl Transferase Activity in Systems with Homopolymer Substrates","authors":"A. B. Sachanka, V. V. Shchur, S. A. Usanov, A. V. Yantsevich","doi":"10.1134/S0003683824605237","DOIUrl":"10.1134/S0003683824605237","url":null,"abstract":"In the current work, single strand binding protein from E. coli (EcSSB) and DNA-binding protein from S. solfataricus (Sso7d) were tested to evaluate the effects on TdT activity for homopolymer substrates (Tn) that are unable to form double helix structures. We showed a significant increase in TdT activity after the addition of EcSSB even from the example of homopolymer substrates. The effects demonstrated open application of DNA binding proteins in TdT engineering and DNA-printing. The addition of EcSSB to the reaction mixture led to a significant increase in TdT activity and a shift in the reaction products towards longer oligonucleotides. The maximum effect was observed in a close-to-equimolar stoichiometric ratio (EcSSB)4:TdT in the presence of Mn2+ cations. In addition, the presence of Sso7d in the reaction mixture led to a slight (up to 15%) decrease in TdT activity for substrates T5 and T15 and a more pronounced decrease for T35 (up to 30%). At the same time, Co2+ cations reduced the inhibitory effect of Sso7d.The patterns and relationships established through our research have potential applications in various fields. Specifically, they can be utilized in protein engineering for the development of fusion proteins that are based on TdT. Furthermore, these findings can contribute to the advancement of novel enzymatic principles for de novo DNA synthesis.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1104 - 1117"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Ethanol on Growth of the Red Microalga Galdieria sulphuraria 乙醇对红色微藻 Galdieria sulphuraria 生长的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605833

Yu. V. Bolychevtseva, I. N. Stadnichuk

{"title":"Effect of Ethanol on Growth of the Red Microalga Galdieria sulphuraria","authors":"Yu. V. Bolychevtseva, I. N. Stadnichuk","doi":"10.1134/S0003683824605833","DOIUrl":"10.1134/S0003683824605833","url":null,"abstract":"Polyextremophilic red microalgae of the genus Galdieria, which inhabit hot sulfur springs under conditions unusual for eukaryotes, are capable of heterotrophy. Among the dozens of exogenous organic substrates identified for Galdieria, ethanol is not mentioned as a possible energy source. It turned out that ethanol did not alter the growth of the model species Galdieria sulphuraria when grown in the dark. By contrast, the growth of microalgae is activated in the light, despite the known cell stressor effect of ethanol. The effect of ethanol as an oxidative stress factor has been indicated by the increase in cellular respiration observed in the dark and also in the light even before the activation of photosynthesis. The marked acceleration of growth of G. sulphuraria culture in the light is most likely due to the stimulation of respiration by ethanol with generation of CO2 and its use by chloroplasts as an additional carbon substrate during the photosynthetic process. Compared to the classical organic substrate glucose, the light-induced growth of G. sulphuraria culture in the presence of ethanol is less intense. It can be speculated that ethanol stress in light induces a system of two consecutive key enzymes in the primary alcohol metabolism chain (alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase), which then leads to the eventual complete oxidation of ethanol, resulting in accelerated growth of G. sulphuraria.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1177 - 1186"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: Plant Growth-Promoting Microbes for Sustainable Crop Production (A Review) 勘误：促进植物生长的微生物促进可持续作物生产（综述）

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824030037

Jyotirmayee Das, Madhusmita Panigrahy, Shraddha Mohanty, Bandita Jena, R. K. Nayak, A. K. Shukla

引用次数: 0

Indolyl-3-Acetic Acid for Medical Use (Review)

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824604670

L. I. Arabova, L. V. Chumikina, R. I. Arabov, A. F. Topunov

{"title":"Indolyl-3-Acetic Acid for Medical Use (Review)","authors":"L. I. Arabova, L. V. Chumikina, R. I. Arabov, A. F. Topunov","doi":"10.1134/S0003683824604670","DOIUrl":"10.1134/S0003683824604670","url":null,"abstract":"Therapeutic effect of many plants is associated with the presence of various bioactive substances in them. The plant hormones—phytohormones—can be distinguished among these substances. When ingested into the human body, they affect physiological processes such as inflammation, glucose uptake, cell division, and resistance to diseases. It is hypothesized that phytohormones may be used as drugs with no side effects. One of them could be the phytohormone indolyl-3-acetic acid (IAA), which is a heteroauxin and can be synthesized by some microorganisms and animals, including mammals. This phytohormone is an important derivative of indole, which is catabolized from dietary tryptophan by the intestinal microbiota. IAA can scavenge free radicals, inhibit oxidative stress, and reduce the production of proinflammatory cytokines. As a ligand of the aryl hydrocarbon receptor, IAA can regulate intestinal homeostasis and suppress inflammatory responses. This review summarizes the current literature data on the vital role of IAA in the treatment of cancer, type 2 diabetes, rheumatoid arthritis, liver disease, Alzheimer’s disease, dental diseases, and microbial skin diseases. The purpose of this review is to draw attention and interest to IAA as a promising therapeutic drug.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"993 - 1009"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Participation of Reactive Oxygen Species and Nitric Oxide in Defense of Wheat Carrying Sr25 Gene Against Stem Rust 活性氧和一氧化氮参与携带 Sr25 基因的小麦对茎锈病的防御

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605298

V. V. Knaub, L. Ya. Plotnikova

{"title":"Participation of Reactive Oxygen Species and Nitric Oxide in Defense of Wheat Carrying Sr25 Gene Against Stem Rust","authors":"V. V. Knaub, L. Ya. Plotnikova","doi":"10.1134/S0003683824605298","DOIUrl":"10.1134/S0003683824605298","url":null,"abstract":"The role of reactive oxygen species (ROS) and nitric oxide (NO) in the defense of Triticum aestivum L. (common wheat) against the rust fungus Puccinia graminis f. sp. tritici Erikss. and Henn. (Pgt) was studied using the example of the interaction between the resistant line of the cultivar Thatcher carrying the Sr25 gene from the tall wheatgrass Thinopyrum ponticum (TcSr25) and the susceptible cultivar Saratovskaya 29 (C29). The seedlings were treated with salicylic acid (SA) as an ROS inducer, verapamil as a Ca2+ channel inhibitor, sodium nitroprusside (NP) as a NO donor, and 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (c-PTIO) as NO scavenger. Isolates with reaction 0 (immunity) and 1 (resistance with hypersensitive reaction, HR) were used to infect the seedlings. NO stimulated the growing tube orientation and the formation of the Pgt appressoria on the surface of resistant plants, as well as increased colony growth in susceptible plants if plants were treated one day before or simultaneously with infection. The generation of superoxide anion was the main cause of Pgt appressoria death on the stomata of resistant plants, while NO did not affect tissue penetration. ROS induced HR and accelerated the destruction of the cell cytoplasm, and NO contributed to the expansion of the necrosis zone in resistant plants.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1301 - 1312"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Endophytic Bacteria Bacillus subtilis 26D and Bacillus velezensis M66 on Resistance of Potato Plants to the Causative Agent of Early Blight Alternaria solani 内生细菌枯草芽孢杆菌 26D 和枯草芽孢杆菌 M66 对马铃薯植株抵抗早疫病病原菌 Alternaria solani 的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605080

A. V. Sorokan, V. F. Gabdrakhmanova, I. S. Mardanshin, I. V. Maksimov

{"title":"Effect of Endophytic Bacteria Bacillus subtilis 26D and Bacillus velezensis M66 on Resistance of Potato Plants to the Causative Agent of Early Blight Alternaria solani","authors":"A. V. Sorokan, V. F. Gabdrakhmanova, I. S. Mardanshin, I. V. Maksimov","doi":"10.1134/S0003683824605080","DOIUrl":"10.1134/S0003683824605080","url":null,"abstract":"The effect of Bacillus velezensis M66 and Bacillus subtilis 26D bacteria on the resistance of potato plants to the necrotrophic fungus Alternaria solani (the causative agent of early blight) was studied. The accumulation of viable bacterial cells of these strains in the internal tissues of the potato stem, roots, and tubers over a long period of time was demonstrated for the first time. A significant reduction in the area affected by the early blight on leaves inoculated with plant endophytes, as well as inhibition of the pathogen growth under the action of bacterial strains, was detected, which can be explained by the synthesis of lipopeptide antibiotics (the genes responsible for the synthesis of which were found by the PCR method) and proteolytic enzymes (the activity of which was demonstrated in vitro). The formation of plant resistance under the influence of inoculation with B. subtilis 26D and B. velezensis M66 was accompanied by an accumulation of hydrogen peroxide in the first hours after plant infection with A. solani spores and by a decrease in this index at the late stages of pathogenesis due to an increase in the activity of catalase and peroxidases. Limitation of the fungus spread was accompanied by an increase in the activity of proteinase inhibitors in plants, which probably decreased the negative effect of proteolytic enzymes of the necrotrophic pathogen A. solani. It can be assumed that the inoculation of plants with bacterial cells of the B. velezensis M66 strain contributed to the formation of resistance of potato plants to the early blight by efficient priming of the phytoimmune potential, which is comparable to the B. subtilis 26D strain (successfully used in the field conditions), an active component of the Fitosporin-M biopreparation.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1313 - 1320"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683824605080.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of a Wood Substrate on the Profile of Volatile Organic Compounds Produced by White Rot Fungus Trametes hirsuta LE-BIN 072 木材基质对白腐菌 Trametes hirsuta LE-BIN 072 产生的挥发性有机化合物的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605821

A. V. Shabaev, T. V. Fedorova

引用次数: 0

Enhancing Disease Resistance in Plants and Stored Potato Tubers through Inoculation of Seed Tubers with the Endophyte Bacillus subtilis 10-4 and Genomic Analysis of its Antimicrobial Properties

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605304

L. I. Pusenkova, O. V. Lastochkina

{"title":"Enhancing Disease Resistance in Plants and Stored Potato Tubers through Inoculation of Seed Tubers with the Endophyte Bacillus subtilis 10-4 and Genomic Analysis of its Antimicrobial Properties","authors":"L. I. Pusenkova, O. V. Lastochkina","doi":"10.1134/S0003683824605304","DOIUrl":"10.1134/S0003683824605304","url":null,"abstract":"The effect of inoculation of healthy hydroponic seed mini-tubers and super-elite reproduction seed tubers with the endophytic bacterium Bacillus subtilis 10-4 on the resistance of plants and stored tubers to diseases was studied. A search for clusters of genes associated with the synthesis of antimicrobial secondary metabolites in the genome of strain 10-4 was carried out. A prolonged protective effect of 10-4 against diseases of leaves (alternariosis) and stored tubers (scab, fusarium) of potatoes was revealed. Metagenomic analysis of the ITS region showed a decrease in the species composition and the proportion of phytopathogenic fungi in plant leaves and stored tubers under the influence of 10-4 with maximum effect during inoculation of healthy mini-tubers. Using the anti-SMASH program, the gene clusters responsible for the synthesis of a wide range of secondary metabolites with antimicrobial activity (type III polyketides, nonribosomal peptide synthetases, betalactones, sactypeptides, rantipeptides, nonribosomal peptide metallophores, transAT-polyketide synthetases, and terpenes) were identified in the genome of strain 10-4. It was revealed that the main antimicrobial compounds of 10-4, which play an important role in the biocontrol of diseases and plant protection, are surfactin, fengycin, bacillibactin, bacillaene, subtilosin A, and bacilysin. In addition, using the CARD database, a wide range of antimicrobial resistance genes with different mechanisms of action were predicted in strain 10-4. In general, the method of pre-sowing treatment of healthy hydroponically grown seed mini-tubers with 10-4 increases the efficiency of bacterial inoculation and ensures long-term protection of tubers during storage and reduces the risk of disease transmission to new generations of plants.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1338 - 1350"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0