Applied Biochemistry and Microbiology最新文献

{"title":"Antioxidant Effect of Carnosine and Carnosine Dinitrosyl Iron Complexes under the Conditions Modeling Peroxidation of Biomolecules","authors":"E. I. Nasybullina,&nbsp;O. V. Kosmachevskaya,&nbsp;K. B. Shumaev,&nbsp;A. F. Topunov","doi":"10.1134/S0003683824604529","DOIUrl":"10.1134/S0003683824604529","url":null,"abstract":"<p>The antioxidant activity of carnosine and of carnosine dinitrosyl iron complexes (DNICs) was studied. A system with metmyoglobin (metMb) or hemin in combination with <i>tert</i>-butyl hydroperoxide (<i>t</i>-BOOH) was used as an experimental model. Using the luminol-dependent chemiluminescence method, it was shown that carnosine and carnosine DNICs effectively diminished the level of prooxidants formed by the interaction of heme groups with <i>t</i>-BOOH. In addition, carnosine and carnosine DNICs inhibited the formation of diene conjugates arising during the oxidation of arachidonic acid in the metMb–<i>t</i>-BOOH system. In the reaction systems used, the antioxidant effect of carnosine DNICs was higher than that of carnosine. The antioxidant effect of carnosine also depended on the presence of bivalent iron ions added at a concentration equivalent to their content in DNICs. These results show that the insertion of carnosine as a ligand to nitrosyl iron complexes enhances its antioxidant properties.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Neurotransmitters on the Fatty Acid Composition and the Pigments of the Green Microalga Scenedesmus quadricauda","authors":"B. Cao,&nbsp;O. B. Chivkunova,&nbsp;A. E. Solovchenko,&nbsp;E. S. Lobakova,&nbsp;A. V. Oleskin","doi":"10.1134/S0003683824604554","DOIUrl":"10.1134/S0003683824604554","url":null,"abstract":"<p>Apart from their functions in the nervous system of animals, neurotransmitters operate as regulatory agents and signals in diverse kingdoms of life. Some neurotransmitters have recently been revealed to exert specific effects on microalgae, predominantly functioning as algal growth stimulators. This article presents new data on the effects of such neurotransmitters as serotonin, norepinephrine, dopamine, histamine, and acetylcholine on the fatty acid and pigment composition of the green microalga <i>Scenedesmus quadricauda</i> (Turp.) Breb. K-1149. It was established that acetylcholine and, to a lesser extent, histamine increased the total fatty acid content of <i>S. quadricauda</i> cells, whereas serotonin and dopamine decreased the fatty acid content. Acetylcholine, histamine, and norepinephrine elevated the percentage of polyunsaturated fatty acids; in contrast, serotonin and dopamine increased the share of saturated fatty acids. Acetylcholine and, to a lesser extent, norepinephrine increased the total chlorophyll content per gram of dry weight in <i>S. quadricauda,</i> while histamine decreased the chlorophyll content. Histamine also increased the chlorophyll <i>a</i>/chlorophyll <i>b</i> and carotenoid/chlorophyll ratios, which were decreased by dopamine. The data obtained are of biotechnological and ecological interest. The stimulation of fatty acid accumulation and the increase in the percentage of polyunsaturated species was caused by the neurotransmitters acetylcholine and histamine at low (1–10 μM) concentrations, which potentially enables facilitating the biotechnological production of health-promoting preparations for therapeutic and cosmetic purposes. However, other neurotransmitters (dopamine and serotonin) tested increased the relative content of saturated fatty acids; therefore, they apparently can be used to stimulate biofuel production, since saturated fatty acid-rich lipids are advantageous raw materials for biodiesel production. The impact of neurotransmitters on microalgal fatty acid composition and photosystem components may be considered in terms of ongoing chemical interaction between microalgae and other aquatic ecosystem components that are known to produce neurotransmitters.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683824604554.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Immunoassay for Detection of Engineered Endolysin LysAm24-SMAP","authors":"A. A. Klimova,&nbsp;I. V. Grigoriev,&nbsp;D. V. Vasina,&nbsp;M. N. Anurova,&nbsp;V. A. Gushchin,&nbsp;N. P. Antonova","doi":"10.1134/S0003683824604384","DOIUrl":"10.1134/S0003683824604384","url":null,"abstract":"<p>In recent years modified bacteriophage lysins have widely been investigated for the purposes of development of antibacterial therapy. Thus, effective and precise methods for the quantitative analysis of these enzymes are in high demand. The enzyme-linked immunosorbent assay (ELISA) method has been developed for the detection of recombinant modified endolysin LysAm24-SMAP in biological samples. The optimal parameters for protein detection were determined, in particular, the influence of salt and the composition of the buffer system for preparation of the samples was studied. The applicability of the immunodetection system of the genetically engineered endolysin LysAm24-SMAP in various biological samples with enzyme concentrations from 0.4 ng/mL was demonstrated. In addition, the influence of matrix effects in samples of animal organs and tissue homogenates and producer strain lysates and their individual components during the analysis was assessed and it was shown that 0.65 M NaCl addition in the ELISA buffer is crucial for achieving correct results and reduces nonspecific interactions in the case of LysAm24-SMAP. The effectiveness of the developed system in the immunochemical control of the bacteriolytic enzyme was confirmed.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of the Pseudomonas sp. OBA 2.4.1 Strain for Presowing Treatment of Pea Seeds (Pisum Sativum L.) in the Presence of Heavy Metals and Glyphosate","authors":"L. R. Khakimova,&nbsp;O. V. Chubukova,&nbsp;Z. R. Vershinina","doi":"10.1134/S0003683824604414","DOIUrl":"10.1134/S0003683824604414","url":null,"abstract":"<p>The results of study of the effects of the <i>Pseudomonas</i> sp. OBA 2.4.1 PGPB strain resistant to NiCl₂ (up to 3 mM), Pb(CH₃COO)₂ (up to 5 mM), and glyphosate (up to 8 mg/mL) on <i>Pisum sativum</i> L. plants at different concentrations of the heavy metals and the herbicide are reported. It was found that this strain had a positive effect on the root length of pea seedlings in the presence of heavy metals indicating an increase in the plant resistance to nickel and lead stress. However, no such effect was observed in the experiment with glyphosate, which confirmed the high toxicity of this compound. The results obtained demonstrated that the <i>Pseudomonas</i> sp. OBA 2.4.1 strain promoted the growth of <i>Pisum sativum</i> L. under nickel and lead stress; these findings can further be used for the development of complex biopreparations intended both for the protection of agricultural plants from the effects of heavy metals and for remediation of contaminated soils.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Yersinia pestis Antigens in the Reception of Plague Diagnostic Bacteriophage L-413C","authors":"A. A. Byvalov,&nbsp;L. G. Dudina,&nbsp;T. B. Kravchenko,&nbsp;S. A. Ivanov,&nbsp;I. V. Konyshev,&nbsp;N. A. Morozova,&nbsp;A. V. Chernyadiev,&nbsp;S. V. Dentovskaya","doi":"10.1134/S0003683824604438","DOIUrl":"10.1134/S0003683824604438","url":null,"abstract":"<p>The role of surface antigens of <i>Yersinia pestis</i> in reception of the phage L-413C was evaluated experimentally. Based on the methods of phage inactivation after its co-incubation with soluble or bead-bounded antigens, the importance of the plague microbe LPS in the phage reception was confirmed, and the inability to bind the capsular antigen F1, Ail protein, and two autotransporters YapF and YapM was shown. The native and recombinant PsaA, being solved, significantly inhibited the lytic activity of the phage in contrast to the bead-bound antigens. The knockout EV cells (Δ<i>psaA</i>) are able to bind the phage particles as well as the wild strain. The use of three methods to evaluate the role of the PsaA antigen in phage L-413C reception gave contradictory results. On the one hand, the reactive domains of PsaA are able to interact with phage particles in solution. At the same time, these domains appear to determine nonspecific binding of the PsaA protein to the underlying bacterial cell structures or polystyrene microsphere, preventing phage adhesion.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of the Biosynthesis of Butyric Acid from Glucose through the Inverted Fatty Acid β-Oxidation Pathway by Recombinant Escherichia coli Strains","authors":"A. Yu. Gulevich,&nbsp;A. Yu. Skorokhodova,&nbsp;V. G. Debabov","doi":"10.1134/S0003683824604360","DOIUrl":"10.1134/S0003683824604360","url":null,"abstract":"<p>The biosynthesis of butyric acid from glucose through inverted fatty acid β-oxidation by recombinant <i>Escherichia coli</i> strains was optimized. An increased yield of the target compound was achieved resulting from the plasmid expression of the <i>atoB</i>, <i>fadB,</i> and <i>fadE</i>/<i>fabI</i> genes in the core strain MG∆4 P_L-<i>tesB</i> Δ<i>yciA</i> (MG1655 ∆<i>ackA-pta</i>, ∆<i>poxB</i>, ∆<i>ldhA</i>, ∆<i>adhE</i>, P_L-SD_ϕ10-<i>tesB</i>, ∆<i>yciA</i>). The positive effect of enforced ATP hydrolysis on microaerobic conversion of the carbohydrate substrate to the final product by the recombinants was demonstrated. Activation of the futile cycle of pyruvate–phosphoenolpyruvate–pyruvate, due to the increased expression of the <i>ppsA</i> gene, ensured a marked increase in glucose consumption by the recombinants and led to an increase in the molar yield of butyric acid up to 39.5%. When the components of the H⁺-ATP synthase complex were uncoupled resulting from the deletion of <i>atpFH</i> genes, the molar yield of butyric acid from glucose demonstrated by the strain forming butyryl-CoA by the action of enoyl-ACP reductase FabI reached 46%.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platinum Nanoparticles in Aqueous Solutions of a Chitosan–Vinylpyrrolidone Copolymer: Synthesis and Biological Activity","authors":"D. N. Zuev,&nbsp;E. I. Cherkasova,&nbsp;K. V. Apryatina,&nbsp;S. D. Zaitsev,&nbsp;L. A. Smirnova","doi":"10.1134/S0003683824604451","DOIUrl":"10.1134/S0003683824604451","url":null,"abstract":"<p>Grafted copolymers of chitosan–vinylpyrrolidone, water-soluble at a pH of 6.8–7.5, were obtained. A technique has been developed for obtaining an aggregatively stable system of platinum nanoparticles in copolymer solutions with an average size of ~4 nm. The thermophysical and structural characteristics of the powdered composition of a platinum nanoparticle-copolymer are investigated. An in vitro comparison of the antitumor activity of solutions of the developed composition and cisplatin at the same platinum concentration was performed. It was found that with respect to the culture of HeLa Kyoto and A431 cancer cells, the composition is five and two times less effective than cisplatin, respectively. Along with this, the biocompatibility of the composition is 17 times higher than that of cisplatin, which allows its use at elevated concentrations and the development of an antitumor agent with platinum nanoparticles commensurate in effectiveness with cisplatin.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Expression of Crm197: Investigation and Optimization of Gene Expression Factors for Effective Production in E. coli","authors":"S. O. Rogozhkin,&nbsp;A. S. Gerasimov","doi":"10.1134/S000368382460444X","DOIUrl":"10.1134/S000368382460444X","url":null,"abstract":"<p>CRM197 (<b>C</b>ross <b>R</b>eacting <b>M</b>aterial 197) is an inactive form of the <i>C. diphtheriae</i> exotoxin used as a carrier protein for the development and production of conjugated polysaccharide vaccines and immunotherapeutic drugs. However, the development of these research areas is not possible without an efficient and cost-effective technology to produce CRM197 of the proper quality. In this study, we developed a highly efficient method to produce recombinant CRM197 as a fusion with SUMO protein, yielding more than three grams per liter in the form of inclusion bodies. We examined the significant effect of the type of expression vector, the heterologous gene expression conditions, and cultivation on its solubility. Using a combination of reduced cultivation temperature and the promoter of the gene encoding the heat shock protein CspA, we achieved an increase in the solubility level of SUMO-CRM197 of more than 30%, with an overall biosynthesis level of more than two grams per liter. Coexpression of the target gene with the DsbC disulfide isomerase gene allowed us to obtain the target protein completely in the soluble state with a yield of more than 1.4 grams per liter. The results obtained may become the basis for the development of a promising domestic technology for the production of CRM197.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and Characterization of Endophytic Bacteria Isolated from the Rhizosphere of Pea Seedlings (Pisum Sativum L.)","authors":"L. E. Makarova,&nbsp;Yu. A. Markova,&nbsp;Yu. V. Zaytseva,&nbsp;A. A. Bychkova,&nbsp;I. V. Gorbenko,&nbsp;Yu. M. Konstantinov,&nbsp;I. A. Vasiliev,&nbsp;A. S. Morits,&nbsp;P. A. Bizikov","doi":"10.1134/S0003683824604426","DOIUrl":"10.1134/S0003683824604426","url":null,"abstract":"<p>Previously it was shown that endophytic bacteria had the ability to move out of the roots of pea plant seedlings (<i>Pisum sativum L</i>.) into the rhizosphere. In this study, six distinct bacterial strains were isolated from the root growth medium during the cultivation of seedlings in an aqueous medium. By analyzing the nucleotide sequence of 16S rRNA genes, the taxonomic position of these strains was established. Their morphological and cultural parameters were assessed, and the activity of hydrolytic enzymes (pectinase, cellulase, protease) and the IAA-producing capability were examined. It has been observed that the quantity of endophytic bacteria that appears on the root surface during the growth of pea seedlings significantly surpasses the quantity present in the root tissues. It is assumed that hydrolytic enzymes such as pectinase and cellulase are involved in the release of bacteria into the external environment, causing the destruction of carbohydrate structures in plant cell walls. The metabolic parameters established in the studied strains, and the significance of these endophytic bacteria for the host plant after their exit from the roots into the rhizosphere are under discussion.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Organic Solvents on the Results of Immunoenzyme Determination of Herbicide Butachlor: Selection of Sample Preparation Modes","authors":"A. N. Berlina,&nbsp;N. I. Smirnova,&nbsp;N. S. Komova,&nbsp;K. V. Serebrennikova,&nbsp;A. V. Zherdev,&nbsp;B. B. Dzantiev","doi":"10.1134/S0003683824604359","DOIUrl":"10.1134/S0003683824604359","url":null,"abstract":"<p>The influence of organic solvents such as methanol and acetonitrile on the results of butachlor immunoassay in samples of rice and rice products was studied. The schemes of enzyme immunoassay using: (a) native antiserum containing specific antibodies to butachlor and antispecies antibodies labeled with horseradish peroxidase, and (b) biotinylated specific antibodies and streptavidin labeled with peroxidase are considered. The close values of IC10 (0.77 and 0.68 ng/mL, respectively) and working range (2.6–165 and 2.4–192 ng/mL, respectively) were established for the two schemes, when analyzing in a mixture of phosphate buffer and methanol in a percentage ratio of 85 : 15, respectively. For the second scheme, the detection of butachlor in butachlor in rice-containing products is shown at 80-132% of the added analyte. A comparison of sample preparation methods allows us to recommend: (a) extraction of butachlor with hexane and re-dissolution of the dry residue in a buffer with 15% methanol or (b) preparation of a methanol extract followed by 6-fold dilution with a buffer solution.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}