Applied Biochemistry and Microbiology最新文献_第2页

Phage Antibodies for Kanamycin Detection 卡那霉素噬菌体抗体的检测

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050071

O. I. Guliy, S. S. Evstigneeva, S. A. Staroverov, A. S. Fomin, O. A. Karavaeva

引用次数: 0

Enhancement of Biomethane Generation from Coal by Anaerobically Co-Degrading with Biological and Chemical Products of Straw Treatment 秸秆处理生化产物厌氧共降解提高煤中生物甲烷的生成

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050101

Y. Liu, Y. Cheng, Y. Li, H. Guo, Z. Huang, M. Urynowicz

{"title":"Enhancement of Biomethane Generation from Coal by Anaerobically Co-Degrading with Biological and Chemical Products of Straw Treatment","authors":"Y. Liu, Y. Cheng, Y. Li, H. Guo, Z. Huang, M. Urynowicz","doi":"10.1134/S0003683823050101","DOIUrl":"10.1134/S0003683823050101","url":null,"abstract":"Co-degradation of coal and straw is an effective way to enhance the generation of biogenic coalbed methane (CBM). However, solid straw is not easily injected into coal seam and the complex and rigid structure of straw also makes the direct degradation inefficient. Therefore, straw was biologically and chemically treated, here, to obtain a liquid product which was further co-degraded with coal to produce methane. The results showed that the methane productions in co-degradations of coal and liquid products were all higher than that from coal. The highest methane production was observed in co-degradation of coal and biological liquid products with fresh microflora (MRC1) which was about 600 μmol and even higher than that in co-degradation of coal and straw. Organic acids were dominant in all liquid products whose contents ranged from 25.4 to 73.1%, and were positively related with methane productions in co-degradation. After co-degradation, the microbial community in MRC1 was closer to the original microflora, and the coal sample was degraded deeper than after cultivation with only coal as revealed by FTIR results. These results suggested that the liquefaction of straw by pretreatment can perfectly solve the injection and degradation limitation in co-degradation, and organic acids would be the essential factors in the interaction between coal and straw. Based on that, a new method was proposed to enhance biogenic CBM generation in coal seam or goaf by injecting microorganisms and liquid products or organic acids associated with rice straw.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"694 - 705"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the Biochemical Properties of Recombinant Alpaca (Vicugna pacos) Chymosins Produced in Pro- and Eukaryotic Expression Systems 重组羊驼Chymosis在原核和真核表达系统中的生化特性比较

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050034

S. V. Belenkaya, V. V. Elchaninov, V. Y. Chirkova, D. N. Shcherbakov

{"title":"Comparison of the Biochemical Properties of Recombinant Alpaca (Vicugna pacos) Chymosins Produced in Pro- and Eukaryotic Expression Systems","authors":"S. V. Belenkaya, V. V. Elchaninov, V. Y. Chirkova, D. N. Shcherbakov","doi":"10.1134/S0003683823050034","DOIUrl":"10.1134/S0003683823050034","url":null,"abstract":"<div>Based on the yeast Kluyveromyces lactis, a strain-producer of recombinant alpaca (Vicugna pacos) prochymosin has been developed. A comparative analysis of the biochemical properties of recombinant alpaca chymosin obtained in the expression systems of K. lactis and Escherichia coli has been carried out. It was found that the recombinant alpaca chymosin synthesized in K. lactis exceeds the analogue obtained in E. coli by 12.9 times in the turnover number of the enzyme, and by 2.9 times in catalytic efficiency. Compared to chymosin expressed in E. coli, the enzyme obtained in a eukaryotic producer has a thermal stability threshold increase of 5°C. Replacing a prokaryotic producer with a eukaryotic one enhances the negative sensitivity of the milk-clotting activity of recombinant alpaca chymosin to an increase in substrate pH in the range of 6.1–6.9, which is accompanied by an increase in the duration of coagulation by 8–35%. With an increase in the concentration of CaCl2 in the substrate, the coagulation activity of the target enzyme synthesized in E. coli was 12–14% higher than that of its analogue obtained in K. lactis.</div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"630 - 635"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic Insights of Colistin Resistance and Its Public Health Implications 粘菌素耐药性的机制研究及其公共卫生意义

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050022

A. Ahsan, S. Gull, H. Imran, Z. Khan

{"title":"Mechanistic Insights of Colistin Resistance and Its Public Health Implications","authors":"A. Ahsan, S. Gull, H. Imran, Z. Khan","doi":"10.1134/S0003683823050022","DOIUrl":"10.1134/S0003683823050022","url":null,"abstract":"Multi-drug resistance poses a serious threat to the public health worldwide. The situation becomes worse when multidrug-resistant bacteria develop resistance against last resort antibiotics such as colistin attributed to plasmid-borne mcr genes. Humans and non-human animals including pigs and chickens are the main reservoirs of these mcr genes harboring bacteria which have now disseminated globally, thus, causing difficulties in treating bacterial infections. Several mcr-gene-containing bacterial (MGCB) species have been found in diverse ecosystems, including aquatic, soil, botanical, wildlife, animal, and public environments. Environmental samples have shown to contain the mcr-1, mcr-2, mcr-3, mcr-5, mcr-7, and mcr-8 genes variants. The genes are found in Escherichia coli, Enterobacter, Klebsiella, Proteus, Salmonella, Citrobacter, Pseudomonas, Acinetobacter, Kluyvera, Aeromonas, Providencia, and Raulotella isolates. The routes of transmission of MGCB are contact with reservoirs that contain mcr, consumption of contaminated animal-/plant-based foods or water, trade and travel that involve animal-/plant-based foods. An updated and precise estimation of prevelance as well as mechanism of colistin resistance mediated by mcr genes is inevitable to regulate the spread of colistin resistance by implementing effective strategies. Hence, this review presents a comprehensive overview of epidemiology and the various mechanisms of colistin resistance that are currently not clearly understood.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"597 - 607"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms Underlying the Virulence and Intestinal Colonization of the Vibrio alginolyticus HY9901 DctP Protein with Proteomic Analysis 溶藻弧菌HY9901 DctP蛋白的毒力和肠道定殖机制的蛋白质组学分析

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050198

Y. L. Zhang, F. Wu, Y. C. Huang, J. C. Jian, S. H. Cai

{"title":"Mechanisms Underlying the Virulence and Intestinal Colonization of the Vibrio alginolyticus HY9901 DctP Protein with Proteomic Analysis","authors":"Y. L. Zhang, F. Wu, Y. C. Huang, J. C. Jian, S. H. Cai","doi":"10.1134/S0003683823050198","DOIUrl":"10.1134/S0003683823050198","url":null,"abstract":"Vibrio alginolyticus as a common pathogen infecting humans and marine animals has become a severe threat to the global mariculture industry. Using liquid chromatography and tandem-mass spectrometry (LC–MS/MS) analysis, comparative proteomics between dctP-deletion (ΔdctP) strain and wild-type V. alginolyticus HY9901 was profiled, and total of 547 differentially expressed proteins (DEPs) were identified. In these DEPs, 310 proteins were upregulated and 237 proteins were downregulated. Comparative proteomics of ΔdctP and wild-type strains identified some important regulated factors of colonization and virulent proteins. The regulated factors of bacterial colonization mainly included LuxR and ToxR (biofilm formation), flagellins (FlaB and FlrB), mannose-sensitive hemagglutinin (MshA) pilus, various substrates of the type II secretion system (EpsD, SecD and SecF) and type IV secretion system (ClpV), quorum sensing systems (LuxR homologous ValR), the virulent heat shock proteins (IbpA and YbeY), lipopolysaccharide LptE, outer membrane proteins (OmpU and TolC), glutamate synthases (GltB and GltD), oligopeptide permease (OppA) and siderophores (IrgA). In our previous study, biological characteristics (swarming motility, biofilm formation, cell adhesion and colonized ability) were significantly decreased and LD50 was significantly increased of ΔdctP compared to wide-type. Taken together, these results suggest that DctP protein plays an important role in controlling biological characteristics through DEPs in this study to finally affecting intestinal colonization and virulence.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"646 - 658"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combining Metabolomics and Metagenomics to Analyze the Biosynthetic Potential of Culturable Endophytic Fungi Isolated from Salvia miltiorrhiza 代谢组学与宏基因组学相结合分析可培养丹参内生真菌的生物合成潜力

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050095

L. Li, C.-B. Li, Y.-H. Wu, B. Li, Y.-R. Wu, Z.-Y. Yan

{"title":"Combining Metabolomics and Metagenomics to Analyze the Biosynthetic Potential of Culturable Endophytic Fungi Isolated from Salvia miltiorrhiza","authors":"L. Li, C.-B. Li, Y.-H. Wu, B. Li, Y.-R. Wu, Z.-Y. Yan","doi":"10.1134/S0003683823050095","DOIUrl":"10.1134/S0003683823050095","url":null,"abstract":"Plant endophytic fungi are reservoirs of novel bioactive natural products, which may be used directly or indirectly to treat numerous diseases. However, it is difficult to rapidly obtain the natural products of interest from enormous endogenic fungi by conventional chemical extraction and separation methods when the overall biosynthetic potential is unknown. There is a lack of systematic studies on the biosynthetic and metabolic potential of endophytic fungi of Salvia miltiorrhiza. Therefore, untargeted metabolomics and metagenomic binning technology were used in this study to analyze the biosynthetic potential of endophytic fungi isolated from S. miltiorrhiza, including 47 species and 166 strains of 24 genera. Metabolomics analysis showed that 3016 metabolites were annotated, involving multiple primary and secondary metabolic pathways. A total of 12 genera and 443 KEGG pathways were annotated in the metagenomic, involving the biosynthesis of amino acids, saccharides, lipids, terpenoids, alkaloids, and polyketides. At the genus level, the biosynthesis and differences of amino acids, saccharides, alkaloids, terpenoids, phenolic acids and polyketides by endophytic fungi of S. miltiorrhiza were revealed. It provides a basis for quickly searching specific active substances from the endophytic fungi of S. miltiorrhiza and enhancing host plant productivity.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"659 - 672"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Clinical Assessment of the RT-LAMP-Based Colorimetric Diagnostic Method for COVID-19 with Improved Primers Sets 改进引物集的基于RT-LAMP-比色法诊断新冠肺炎的临床评价

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050150

S. Sarkar, D. Balasubramanian, N. Velugonda, N. Ede, M. Srinivas, M. R. Katika, S. Raran-Kurussi, M. Jaiswal

{"title":"A Clinical Assessment of the RT-LAMP-Based Colorimetric Diagnostic Method for COVID-19 with Improved Primers Sets","authors":"S. Sarkar, D. Balasubramanian, N. Velugonda, N. Ede, M. Srinivas, M. R. Katika, S. Raran-Kurussi, M. Jaiswal","doi":"10.1134/S0003683823050150","DOIUrl":"10.1134/S0003683823050150","url":null,"abstract":"A cost-effective and robust diagnosis of an infectious agent is essential during a pandemic outbreak. While RT-qPCR-based methods are widely used, alternative methods that can be used outside the laboratory setup would enable better disease control and widen the surveillance efforts. The loop-mediated isothermal amplification (LAMP) method is an alternate method that has not yet been commercially deployed widely. The colorimetric LAMP can be adapted for various pathogens and would be helpful for high-throughput screening in a limited resource setting. Moreover, it is quicker and less labor-intensive. In our study, improved RT-LAMP assay primer sets were designed to detect SARS-CoV-2: N gene and ORF1a gene fragments. RT-LAMP assay primers were also designed to detect human RNase P to ensure RNA integrity. RT-LAMP reaction tubes/plates were incubated at 65°C for 30 min. Cell phone cameras were used to record results. RT-LAMP results were assessed by comparing the results obtained with RT-qPCR. RT-LAMP detected ~100 RNA copies, and its sensitivity was on par with RT-qPCR. Out of 60 patient samples with extracted RNA, a positive agreement of 98.2% was observed between RT-LAMP and RT-qPCR. On a cautionary note, severe discrepancies were observed if the same oligos were procured from different vendors, drastically affecting the test results. Therefore, mandatory quality control of primers is indispensable.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"733 - 742"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Strategies for Bio-Conjugation of Anti-CD4 Monoclonal Antibodies to Silica-Coated Magnetic Nanoparticles and Their Application for the Positive Selection of CD4+ Lymphocytes 抗CD4单克隆抗体与二氧化硅包覆的磁性纳米粒子生物偶联的不同策略及其在CD4+淋巴细胞阳性选择中的应用

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050083

M. Khorrami, M. Mahmoudi, S. S. Shobeiri, M. Moghadam, M. Sankian

{"title":"Different Strategies for Bio-Conjugation of Anti-CD4 Monoclonal Antibodies to Silica-Coated Magnetic Nanoparticles and Their Application for the Positive Selection of CD4+ Lymphocytes","authors":"M. Khorrami, M. Mahmoudi, S. S. Shobeiri, M. Moghadam, M. Sankian","doi":"10.1134/S0003683823050083","DOIUrl":"10.1134/S0003683823050083","url":null,"abstract":"Efficient cell separation techniques are required for the isolation of various cellular populations in fundamental research. In the present study, five types of magnetic nanoparticles (MNPs) have been designed to isolate CD4+ T cells from peripheral blood mononuclear cells. MNPs were synthesized by a modified solvothermal method and subsequently coated by a silica shell through the sol-gel process. Then, the anti-CD4 antibody was conjugated on the surface of different groups of MNPs, which were functionalized with various cross-linkers (including the carboxylic and aldehyde groups) and proteins (such as protein A and streptavidin). The performance of prepared MNPs for the separation of CD4+ lymphocytes was investigated by optical microscopy and flow-cytometry. This study revealed that the cell isolation rates by different functionalized groups, including aldehyde, carboxyl, CHO-protein A, COOH-protein A, and streptavidin conjugated with nanoparticles, were 32 ± 3, 32 ± 1, 80 ± 4, 70 ± 1, and 71 ± 2%, respectively. Based on such data, the CHO-protein A-functionalized MNPs have been considered a practical choice to separate CD4+ T cells with over 92 ± 3% purity. MNPs obtained in the study provide an adequate tool for the cell isolation method and further present the dramatic potential to be applied to several fields of biomedical purpose.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"706 - 715"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in Fatty Acid Profiles and the Formation of Volatile Organic Compounds During Fermentation of Cow’s Milk with Probiotic Lacticaseibacillus paracasei Strains 副干酪乳杆菌益生菌发酵牛奶过程中脂肪酸组成的变化及挥发性有机物的形成

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI: 10.1134/S0003683823050137

K. V. Moiseenko, A. V. Shabaev, O. A. Glazunova, O. S. Savinova, T. V. Fedorova

{"title":"Changes in Fatty Acid Profiles and the Formation of Volatile Organic Compounds During Fermentation of Cow’s Milk with Probiotic Lacticaseibacillus paracasei Strains","authors":"K. V. Moiseenko, A. V. Shabaev, O. A. Glazunova, O. S. Savinova, T. V. Fedorova","doi":"10.1134/S0003683823050137","DOIUrl":"10.1134/S0003683823050137","url":null,"abstract":"In this work, a comparative analysis of the profile of fatty acids (FA) and volatile organic compounds (VOCs) for skimmed cow’s milk fermented by four different probiotic strains of Lacticasibacillu paracasei (ABK, KF1, MA2, and MA3) was carried out. Analysis of FA and VOC profiles was performed using gas chromatography with mass spectrometric detection (GC-MS). For additional characterization of odor changes and its intensity in the fermented milk samples, the E-nose “electronic nose” odor analyzer was used. In total, the presence of 42 different FAs was detected in all samples, of which 17 were saturated, 8 monounsaturated, and 5 polyunsaturated. The strain-specific differences between the studied samples were of a complex nature and could not be explained only by the variation in the contribution of several individual FAs. The FA-nutritional indices characterizing the nutritional and biological value of the samples were different for milk fermented by different strains. Analysis of VOCs showed that the main odor-forming compounds in the studied samples were FAs and their reaction products, 2-heptanone, 2-nonanone, and 2-nonanol, which formed as a result of the following chain of transformations: β-oxidation → decarboxylation → reduction. The aroma of L. paracasei fermentation products, predicted on the basis of the odors of individual VOCs, generally coincided with their organoleptic assessment, a flavor typical of fermented milk (yogurt and curdled milk) with floral and fruity notes.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"636 - 645"},"PeriodicalIF":0.8,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41085704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of Mitogen-activated Protein Kinase from Schizochytrium sp. and Application in Resisting Stress Environments Schizochytrium sp.丝裂原活化蛋白激酶的鉴定及其在抗逆性环境中的应用

IF 0.8 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI: 10.1134/S000368382304004X

C. Du, J. Jiang, X. Hu, L. Ren

{"title":"Identification of Mitogen-activated Protein Kinase from Schizochytrium sp. and Application in Resisting Stress Environments","authors":"C. Du, J. Jiang, X. Hu, L. Ren","doi":"10.1134/S000368382304004X","DOIUrl":"10.1134/S000368382304004X","url":null,"abstract":"Schizochytrium sp. is a marine fungus, which usually lives in the salinity of 40–100% seawater and has unique salt tolerance characteristics. In this study, we explored the candidate mitogen-activated protein kinase (MAPK) related to the salt-tolerant regulatory network from Schizochytrium sp. Firstly, 6 potential MAPK genes from Schizochytrium sp. were identified based on the genome information and bioinformatics analysis. Then, the MAPK gene expression of Schizochytrium sp. under different cultivation conditions locked two genes encoding Mpk6315 and Mpk2022. Furthermore, overexpressing of Mpk6315 in S. cerevisiae enhanced cell growth by 62% while and Mpk2022 overexpression improved the membrane integrity under different stress conditions, indicating that Mpk6315 might play an important role for cell growth and Mpk2022 could help cells to encounter different environmental stressors. This study was the first time to identify the MAPK genes from Schizochytrium sp., which could enlarge the MAPK modules of salt stress tolerance and provide new elements for improving the stress resistance of other microorganisms.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"438 - 449"},"PeriodicalIF":0.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4787561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0