Applied Biochemistry and Microbiology最新文献_第3页

Acetylation of Proteins in Bacteria as a Method of Regulation of Cell Metabolism 细菌中蛋白质乙酰化作为调节细胞代谢的一种方法

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700030

N. S. Plekhanova, I. B. Altman, V. A. Livshits, M. S. Yurkova, A. N. Fedorov

引用次数: 0

Metabolic Engineering of the Yeast Schizosaccharomyces pombe Tolerant to Low pH for L-Lactic Acid Production 酵母裂糖菌耐低pH产l -乳酸的代谢工程研究

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700133

E. O. Anisimova, M. G. Tarutina, S. P. Sineoky

{"title":"Metabolic Engineering of the Yeast Schizosaccharomyces pombe Tolerant to Low pH for L-Lactic Acid Production","authors":"E. O. Anisimova, M. G. Tarutina, S. P. Sineoky","doi":"10.1134/S0003683824700133","DOIUrl":"10.1134/S0003683824700133","url":null,"abstract":" The recombinant strain based on the acidophilic yeast Schizosaccharomyces pombe with a cloned gene of heterologous lactate dehydrogenase (LDH) was used to optimize the biosynthesis of L-lactic acid. For this purpose, the effect of inactivation of pyruvate decarboxylase genes (PDC) on the synthesis of acetate, pyruvate, and ethanol (the main by-product in the synthesis of lactic acid) was studied. Using the wild S. pombe strain, we showed that the Δpdc3 and Δpdc4 deletions did not affect these indicators, while in the Δpdc1 mutant, ethanol biosynthesis was reduced and acetate biosynthesis was increased, and the Δpdc2 mutant accumulated pyruvate. The effect of deletions of the PDC1 and PDC2 genes on lactic acid biosynthesis was tested on a model S. pombe strain containing a heterologous LDH gene from Lactobacillus pentosus. It was shown that in strains with the inactivated PDC2 gene the accumulated pyruvate was poorly consumed for the biosynthesis of lactic acid even in the presence of two different recombinant lactate dehydrogenases; the introduction of the third gene of heterologous lactate dehydrogenase led to loss of viability of the strain. At the same time, in strains with the deleted PDC1 gene, the biosynthesis of lactic acid was enhanced with the introduction of additional LDH genes. The results obtained can be used in the design of industrial lactic acid producing strains.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1534 - 1542"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Factors of Fungal Virulence in Relation to Heavy Metal Contamination of Soils 土壤重金属污染真菌毒力的分子因子研究

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700108

E. V. Fedoseeva, O. S. Luchkina, V. A. Terekhova

{"title":"Molecular Factors of Fungal Virulence in Relation to Heavy Metal Contamination of Soils","authors":"E. V. Fedoseeva, O. S. Luchkina, V. A. Terekhova","doi":"10.1134/S0003683824700108","DOIUrl":"10.1134/S0003683824700108","url":null,"abstract":"Abstract—Heavy metal (HM) pollution has become one of the important threats to biological processes occurring in soil and to soil microbiota. This threat also extends to the active component of the soil microbial biota—fungi. In soils polluted with HMs, fungal communities undergo significant structural changes, leading, in particular, to an increase in the proportion and diversity of HM-resistant fungi, among which opportunistic human pathogens and facultative plant pathogens are found. The strains of fungi opportunistic for humans isolated from polluted soils have pathogenic properties to a greater extent than the strains isolated from clean soil. The implementation of pathogenic properties is determined by a number of molecular factors (virulence factors), among which are the activity of certain groups of enzymes, the action of antioxidant defense mechanisms and effector proteins, the synthesis of melanin and toxins, and modification of the lipid and carbohydrate composition of the cell. Heavy metals and soil pollution with them can influence the implementation of the pathogenic properties of fungi and fungus-like organisms that are toxic for humans, animals, and plants. This review provides data from the literature on molecular virulence factors and their modifications under the influence of HMs. Considering that at the moment there is no clear understanding of the direction of the action of HMs on the implementation of pathogenic properties by fungi, the literature data analyzed can still contribute to understanding the consequences of soil pollution with HMs for the development of opportunistic human pathogens and facultative phytopathogens.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 7","pages":"1504 - 1516"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Methyl Green in Flow Cytometry to Control the Cell Viability of C. glutamicum 流式细胞术中甲基绿对谷氨酸酵母细胞活力的控制

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700169

A. S. Derunets, D. D. Derbikov, A. S. Yanenko

引用次数: 0

New Complex Enzyme Preparation of Exo-Inulinase and Pectin Lyase for Use in the Jerusalem Artichoke Processing Technology 外链菊粉酶和果胶裂解酶复合酶的制备及其在菊芋加工中的应用

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700054

A. M. Rozhkova, Y. A. Denisenko, E. S. Milova, I. N. Zorov, O. A. Sinitsyna, E. V. Yaroshenko, A. P. Sinitsyn

{"title":"New Complex Enzyme Preparation of Exo-Inulinase and Pectin Lyase for Use in the Jerusalem Artichoke Processing Technology","authors":"A. M. Rozhkova, Y. A. Denisenko, E. S. Milova, I. N. Zorov, O. A. Sinitsyna, E. V. Yaroshenko, A. P. Sinitsyn","doi":"10.1134/S0003683824700054","DOIUrl":"10.1134/S0003683824700054","url":null,"abstract":"Abstract—Fructose–glucose syrups (FGSs) are widely used in the food industry as sweeteners. The use of inulo-containing raw materials, which include the Jerusalem artichoke, for their production leads to an increase in the proportion of fructose in the final product to 80–85%, which allows one to classify FGSs as a natural sweetener. Modern production of FGSs in the Russian Federation is based on the use of the enzyme exo-inulinase (EC 3.2.1.80), which hydrolyzes β-2,1-glycosidic bonds of the inulin molecule to produce fructose and glucose. In practice, an enzyme preparation based on this enzyme is added into fermenters of the extracted Jerusalem artichoke diffusion juice (DJ) before the microfiltration stage. However, in addition to the main polysaccharide, inulin (75–80%), DJ contains pectin (up to 5%), which creates an additional load on the microfiltration unit due to the viscosity of Jerusalem artichoke fermentolysates. Based on the fungal strain Penicillium verruculosum InuPel-10, a complex enzyme preparation (EP) of exo-inulinase/pectin lyase was obtained, which reduces the viscosity of Jerusalem artichoke extracts by 17–20%, while the yield of reducing sugars during hydrolysis using exo-inulinase/pectin lyase EP increased by 10%. The use of the complex EP InuPel-10 in the production of FGSs will reduce the cost of the EP by 10–16% due to the reduction in operating costs associated with a more fully loaded technological line for the production of this EP, in contrast to the production of individual enzymes and their further mixing in the required proportion.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 7","pages":"1465 - 1474"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and Metabolomic Analysis of Cholera Vibrions 霍乱弧菌的蛋白质组学和代谢组学分析

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700182

N. R. Telesmanich, Z. I. Mikashinovich, O. B. Smirnova, E. A. Reshetnikova

{"title":"Proteomic and Metabolomic Analysis of Cholera Vibrions","authors":"N. R. Telesmanich, Z. I. Mikashinovich, O. B. Smirnova, E. A. Reshetnikova","doi":"10.1134/S0003683824700182","DOIUrl":"10.1134/S0003683824700182","url":null,"abstract":" Laboratory diagnostics of cholera and study of the properties of the pathogen Vibrio cholerae have not lost their relevance because of migration of populations and the possibility of importing this infection into our country. The emergence of post-genomic technologies opened a new era in the analysis of proteomes and enzyme systems of pathogens and their hosts during the infectious process. The rational use of these methods allows one to improve modern strategies for cholera express-diagnostics and evaluate the effectiveness of the disease treatment. Here, along with a retrospective analysis of the biochemical and genetic principles of the systematization of V. cholerae strains, new data on their metabolic features, which were obtained using MALDI-TOF, nanofluid, and chromatography-mass spectrometry in combination with 2D-electrophoresis and genetic methods, are presented. The structural features of O-antigens V. cholerae Ogava and Inaba and the mechanism of electron transfer along the respiratory chain are considered. The facts clarifying the role of protein kinases and the phosphorylation cascade in metabolic processes under the action of cholera toxin are presented. The inducing effect of the cholera toxin and hemolysin on IL-10-mediated inflammatory responses is described. New data on the role of the phosphoenolpyruvate system in glucose utilization and anaerobic glycolysis in the induction of cholera toxin are discussed. The introduction of the MALDI-TOF technology for biotyping and identification of microorganisms has led to the creation of a database of virtual collections for mass-spectrometric profiles of V. cholerae strains, and its use allows for identifying taxon-specific proteins and virulence markers of the bacterium. For this review, international and Russian databases were used.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1575 - 1583"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Increase in the Adhesive Ability of Recombinant Spidroins with the Recombinant Adhesive Mussel Foot Protein Mfp3 重组粘接贻贝足蛋白Mfp3对重组蜘蛛蛋白粘接能力的提高

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S000368382470025X

V. G. Bogush, L. I. Davydova, S. E. Cheperegin, K. V. Sidoruk, S. V. Krasheninnikov, M. A. Klinskaya, E. V. Sytina, V. G. Debabov

{"title":"An Increase in the Adhesive Ability of Recombinant Spidroins with the Recombinant Adhesive Mussel Foot Protein Mfp3","authors":"V. G. Bogush, L. I. Davydova, S. E. Cheperegin, K. V. Sidoruk, S. V. Krasheninnikov, M. A. Klinskaya, E. V. Sytina, V. G. Debabov","doi":"10.1134/S000368382470025X","DOIUrl":"10.1134/S000368382470025X","url":null,"abstract":" Previously, it was demonstrated that recombinant spidroins rS1/9 and rS2/12 are able to glue various inorganic and organic materials. It is known that mussel foot proteins have unique adhesive properties associated with a high content of L-3,4-dihydroxyphenylalanine (DOPA), which is formed due to modification of tyrosine residues by the enzyme tyrosinase. We constructed a hybrid protein, which contains recombinant mussel foot protein 3 (Mfp3), as well as the recombinant tyrosinase used for modifying the tyrosine residues in DOPA in all recombinant proteins (rS1/9, rS2/12, and Mfp3 in the composition of the hybrid protein). Such modification led to an enhancement of the adhesive properties of these proteins, while the combination of modified spidroins with Mfp3-DOPA significantly increased their adhesive ability, which was demonstrated in the experiments on gluing various materials. The results obtained indicate the high potential of the hybrid protein containing recombinant modified Mfp3 for use alone or as an additive to recombinant spidroins for medical use.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 9","pages":"1665 - 1673"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome and In Vitro Gut Models 微生物组和体外肠道模型

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700261

D. M. Hushpulian, S. V. Nikulin, A. A. Zakharyants, S. S. Savin, V. I. Tishkov

引用次数: 0

Prospects of Development of Biotechnologies for Citric Acid Production 柠檬酸生产生物技术的发展前景

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S000368382470011X

D. A. Dementev, Yu. A. Rybakov, S. P. Sineoky

{"title":"Prospects of Development of Biotechnologies for Citric Acid Production","authors":"D. A. Dementev, Yu. A. Rybakov, S. P. Sineoky","doi":"10.1134/S000368382470011X","DOIUrl":"10.1134/S000368382470011X","url":null,"abstract":" The biotechnological production of citric acid (CA) is one of the largest and fastest-growing sectors in the global biotechnology market. It is produced in crystalline form and as citrate salts and is widely employed in the food industry, household chemicals, cosmetics, metallurgy, and the petroleum extraction sector. Global CA production exceeds two million tons per year and is increasing by approximately 4% annually. Traditional biotechnological CA production relies on the utilization of mycelial fungi, specifically Aspergillus niger as the producer. Various alternative CA producers, including yeast and bacteria-based systems, have been described. However, from an economic standpoint, they lag behind industrial Aspergillus niger producers. Among the most promising, environmentally friendly, and high-tech CA producers are strains of the yeast Yarrowia lipolytica. The development of competitive biotechnologies for CA production using Y. lipolytica yeast strains with glucose as a carbon source has become feasible due to advancements in understanding the genetic control of CA transport and metabolism within the cell.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1517 - 1525"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids as Auxiliary Means in the Therapy and Prevention of COVID-19: Part II. Potential Targets 黄酮类化合物在COVID-19治疗和预防中的辅助作用：第二部分。潜在的目标

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700285

Ya. F. Zverev, A. Ya. Rykunova

引用次数: 0