Applied Biochemistry and Microbiology最新文献_第3页

Anti-Aggregation Potency of Theophylline Against Thermally Aggregated Human Hemoglobin 茶碱对热聚集的人血红蛋白的抗聚集效力

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825601696

N. K. Ansari, S. Khan, Z. Faridi, A. Naeem

{"title":"Anti-Aggregation Potency of Theophylline Against Thermally Aggregated Human Hemoglobin","authors":"N. K. Ansari, S. Khan, Z. Faridi, A. Naeem","doi":"10.1134/S0003683825601696","DOIUrl":"10.1134/S0003683825601696","url":null,"abstract":"The study of protein misfolding and aggregation has been a breakthrough in the realm of protein biochemistry. Protein aggregation is believed to be an intrinsic property of the polypeptide chain linked to its common peptide backbone. Aggregation of certain proteins into inclusion bodies or plaques is linked to a number of neurodegenerative diseases, including Parkinson’s, Alzheimer’s, and Huntington’s disease. Theophylline is a methylxanthine derivative, commonly found in tea, cocoa beans, and chocolates. In the present study, we have reported that theophylline acts as an inhibitor of haemoglobin (Hb) aggregation. The conformational changes in Hb were studied using turbidity assay, intrinsic fluorescence, Congo red assay, thioflavin-T (ThT) assay, Soret spectroscopy, Rayleigh scattering, and circular dichroism. The rise in ThT fluorescence and Congo red absorbance, concomitant with the 11 nm red shift, validates the formation of Hb aggregated at elevated temperature. These results were further confirmed by the loss of negative minima at 208 and 222 nm, which is a hallmark of α-helix. Hb was further incubated with theophylline taken in increasing concentrations at 70°C for 4 h. The decrease in ThT, Rayleigh, and intrinsic fluorescence, turbidity, Soret, and Congo red absorbance suggested the preventive effect of theophylline in a dose-dependent manner. Inhibitory activity of theophylline against the Hb aggregation may guide us in designing novel therapeutic drugs linked to protein aggregation diseases.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"444 - 452"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Growth Promoting Endophytes Agrobacterium pusense and Bacillus cereus from Lycopersicon esculentum Promotes Growth in Wheat Variety HD 2967 under Irrigated and Non-irrigated Conditions 灌溉和非灌溉条件下，番茄内生菌pusense农杆菌和蜡样芽孢杆菌对小麦hd2967的生长有促进作用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683824608321

S. Jaiswal, P. K. Doharey, S. K. Mishra

{"title":"Plant Growth Promoting Endophytes Agrobacterium pusense and Bacillus cereus from Lycopersicon esculentum Promotes Growth in Wheat Variety HD 2967 under Irrigated and Non-irrigated Conditions","authors":"S. Jaiswal, P. K. Doharey, S. K. Mishra","doi":"10.1134/S0003683824608321","DOIUrl":"10.1134/S0003683824608321","url":null,"abstract":"Adverse environmental stresses lower plant growth and productivity. However, plant growth-promoting endophytes are reported to combat the fatalistic effect of stress by the manufacture of phytohormones, enzyme ACC deaminase, siderophore, hydrogen cyanide, solubilization of minerals, and nitrogen fixation. Six bacterial isolates, two from the roots, three from the stems, and one from the leaves, were taken from Lycopersicon esculentum for investigation. Traits promoting plant growth were examined in all microbial strains. The maximum number of traits were observed in bacterial endophytes identified as Agrobacterium pusense strain LER1F and Bacillus cereus strain LES1B. Both the strains produced indole acetic acid, siderophore, and ammonia. The strains LES1B and LER1F tolerated salt concentrations (NaCl) up to 15 and 10%, respectively. Both strains were further studied for their potential role in promoting a variety properties in wheat HD 2967. These strains have increased the root and shoot length, as well as the fresh and dry weight. However, under non-irrigated conditions, A. pusense strain LER1F promoted root length growth, shoot length, and dry weight more effectively compared with those caused by B. cereus strain LES1B. Our research will help develop new biostimulants, bioinoculants, and biotechnological tools that will improve crop plants’ ability to adapt to drought.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"576 - 585"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzybiotics as Biotechnological Medicines in Wound Healing: Current Strategies and Perspectives 酶制剂作为生物技术药物在伤口愈合：当前的策略和前景

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825603622

A. M. Lendel, N. P. Antonova, D. V. Vasina

{"title":"Enzybiotics as Biotechnological Medicines in Wound Healing: Current Strategies and Perspectives","authors":"A. M. Lendel, N. P. Antonova, D. V. Vasina","doi":"10.1134/S0003683825603622","DOIUrl":"10.1134/S0003683825603622","url":null,"abstract":"The first quarter of the 21st century has been marked by the recognition of antimicrobial resistance as a critical global health challenge, prompting the scientific community to develop innovative strategies against high-risk multidrug-resistant pathogens. Furthermore, since the 20th century, knowledge has been progressively accumulating, enabling an understanding of the nature of chronic infections. These interconnected issues have driven the exploration of novel antibacterial agents as alternatives to conventional antibiotics. Among these, enzybiotics, based on peptidoglycan-degrading enzymes, are particularly promising. However, the active substance of enzybiotics is a double-edged sword because its structural complexity may be beneficial in combating difficult-to-treat infections, but demands precise stabilization and formulation optimization to ensure the efficacy of a final dosage form. Over the past 10 years, significant progress in research has been observed in the development of topical preparations for the treatment of skin and soft tissue infections. In this review, we have analyzed contemporary research focused on the development of formulations for such enzybiotics, identified key R&D directions and challenges, and discussed the importance of these aspects for establishing a pipeline for new generations of biotechnological antibacterial agents.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"401 - 412"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Production and Assessment of Bioavailability of Wood Oligosaccharides for Probiotic Microorganisms 酶法生产木材低聚糖及益生菌微生物生物利用度评价

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825604937

K. A. Mayorova, A. R. Kayumov, I. N. Zorov, A. R. Shevchenko, I. A. Latipova, D. R. Yarullina, A. S. Aksenov

{"title":"Enzymatic Production and Assessment of Bioavailability of Wood Oligosaccharides for Probiotic Microorganisms","authors":"K. A. Mayorova, A. R. Kayumov, I. N. Zorov, A. R. Shevchenko, I. A. Latipova, D. R. Yarullina, A. S. Aksenov","doi":"10.1134/S0003683825604937","DOIUrl":"10.1134/S0003683825604937","url":null,"abstract":"In this work, oligosaccharides from industrial samples of hardwood and softwood kraft pulps were produced enzymatically and characterized in comparison with oat and sugarcane oat xylan oligosaccharides. The highest yield of oligosaccharides by weight of the substrate (up to 7.5%) was achieved by hydrolysis of pulp from a mixture of birch and aspen, catalyzed by domestic Penicillium/Myceliophtora xylanase, cellulase, and mannanase preparations for 1.5 hours at 50°C and pH 5.0. Reducing cellobiohydrolase and β-glucosidase dosage yielded oligosaccharide preparations (more than 70%) with minimal monosaccharide impurities. It was found that the low yield of xylooligosaccharides during the depolymerization of redeposited xylan with kraft pulps by monoenzyme xylanase can be increased by introducing endoglucanases and other cellulases into the reaction mixture. Wood oligosaccharides can be used as components of a nutrient medium for the cultivation of lactobacilli Lactiplantibacillus plantarum (strains FCa3L, S10 and 8PA3) and Pediococcus acidilactici LR-1 and are promising for the development of new synbiotic drugs and can become one of the key vectors for the development of forest bioeconomy.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"496 - 502"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold-Based Nanohybrid Materials in Sensor Systems for Cancer Biomarker Detection and for Tumor Treatment 用于癌症生物标志物检测和肿瘤治疗的传感器系统中的金基纳米杂化材料

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825603464

O. I. Guliy, E. G. Zhnichkova, L. A. Dykman

引用次数: 0

Isolation of Nanochitin from Fungal Mycelium 从真菌菌丝体中分离纳米几丁质

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825602069

L. J. Medina-Palacios, L. M. Silva-Lizarraga, I. Rodriguez-Miranda, L. Chávez-Guerrero, J. Silva-Mendoza

{"title":"Isolation of Nanochitin from Fungal Mycelium","authors":"L. J. Medina-Palacios, L. M. Silva-Lizarraga, I. Rodriguez-Miranda, L. Chávez-Guerrero, J. Silva-Mendoza","doi":"10.1134/S0003683825602069","DOIUrl":"10.1134/S0003683825602069","url":null,"abstract":"The environmental impact of synthetic polymers has driven the search for sustainable alternatives, with biopolymers such as chitin gaining attention due to their biodegradability, biocompatibility, and non-toxicity. Although crustacean shells are commonly used for chitin extraction, they require harsh chemical treatments due to their high mineral and protein content. In this study, filamentous fungi isolated from artisanal bread were cultivated under submerged fermentation to produce mycelial biomass rich in chitin. The extraction process was optimized using hydrogen peroxide at 10 and 20% concentrations under mild thermal conditions. Morphological and structural analyses (SEM, LSCM, FTIR) confirmed that the 20% H2O2 treatment effectively removed non-chitinous materials, yielding nanofibrillar chitin structures with thicknesses around 180 nm. FTIR spectra further indicated a higher structural purity in the peroxide-treated samples. These findings demonstrate that fungal mycelium is a viable and sustainable source of nanochitin, and that hydrogen peroxide is a greener alternative for its extraction compared to conventional acid-base methods.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"526 - 532"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Bacteria of Spruce Picea Jezoensis and their Antagonism Against Plant Pathogens 云杉云杉内生细菌及其对植物病原体的拮抗作用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825603695

A. A. Dneprovskaya, A. A. Ananev, N. N. Nityagovsky, N. S. Primak, K. V. Kiselev, O. A. Aleynova

{"title":"Endophytic Bacteria of Spruce Picea Jezoensis and their Antagonism Against Plant Pathogens","authors":"A. A. Dneprovskaya, A. A. Ananev, N. N. Nityagovsky, N. S. Primak, K. V. Kiselev, O. A. Aleynova","doi":"10.1134/S0003683825603695","DOIUrl":"10.1134/S0003683825603695","url":null,"abstract":"The structure and composition of cultivated endophytic spruce bacteria Picea jezoensis (Siebold & Zucc.) Carrière was established and the antagonistic activity of the main endophytic spruce bacteria P. jezoensis against common plant pathogens was analyzed. The microbiome of P. jezoensis spruce is represented by 4 class-level taxa: Alphaproteobacteria, Actinobacteria, Gammaproteobacteria, and Bacilli. Bacterial representatives of the genus Bacillus, Pseudomonas, and Erwinia have been found to have antagonistic activity against two widespread and dangerous phytopathogenic fungi Pyricularia oryzae and Fusarium sp. The strains that showed antagonistic activity had proteolytic activity, and the R7PjV2-12 strain, which showed a high antagonism index, was able to produce chitinase. The results of this study can become the basis for the development of new, environmentally friendly ways to control phytopathogens and increase plant resistance to diseases, which can be used in forestry and agriculture.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"569 - 575"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological Potential of using Biomass of the Bombyx mori Pupae for Food Production 利用家蚕蛹生物量用于食品生产的生物技术潜力

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683826600739

A. O. Popova, E. V. Kuntina, E. G. Evlagina, R. V. Ulanova

引用次数: 0

Genomic Analysis of Bacteria of the Genus Methylococcus for Defence Systems against Bacteriophages 用于防御噬菌体的甲基球菌属细菌的基因组分析

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S0003683825604688

I. Nizovtseva, A. Chigireva, A. Korenskaia, A. Rezaykin, I. Starodumov

{"title":"Genomic Analysis of Bacteria of the Genus Methylococcus for Defence Systems against Bacteriophages","authors":"I. Nizovtseva, A. Chigireva, A. Korenskaia, A. Rezaykin, I. Starodumov","doi":"10.1134/S0003683825604688","DOIUrl":"10.1134/S0003683825604688","url":null,"abstract":"Methylococcus species utilize methane as the sole carbon and energy source, converting it into biomass and other metabolic end products. Owing to this metabolic capacity, they hold particular promise in industrial C1 biotechnology, especially for the production of protein-rich feed. However, the industrial cultivation of Methylococcus-based consortia on methane is inherently non-sterile, exposing the process to potential biological risks that may compromise the stability, duration and productivity of cultivation. One of the most critical threats is bacteriophage infection, whose triggers for rapid phage-mediated lysis and resulting economic losses remain incompletely understood. Here, we performed a comparative genomic analysis of 9 Methylococcus strains to systematically identify and characterize their antiphage defence systems. We detected between 8 and 15 systems per strain, with restriction–modification (RM), SoFic, CRISPR-Cas, and BREX being the most prevalent. Together, these mechanisms form a conserved multilayered defence core acting at different infection stages. Additional strain-specific systems, including direct phage-sensing and DNA-cleaving, replication-inhibiting, and abortive infection modules, indicate horizontal gene transfer and ecological adaptation. Notably, strains KN2, Mc7, and McNor encode the most diverse and potentially synergistic defence combinations, highlighting their strong protective potential and relevance for stable methane bioconversion under industrial conditions.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"461 - 466"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of Plastics by Insects and the Role of Their Intestinal Microbiota 昆虫对塑料的生物降解及其肠道菌群的作用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-07-08 DOI: 10.1134/S000368382660034X

A. I. Bastrakov, V. A. Zhuikov, N. A. Ushakova

{"title":"Biodegradation of Plastics by Insects and the Role of Their Intestinal Microbiota","authors":"A. I. Bastrakov, V. A. Zhuikov, N. A. Ushakova","doi":"10.1134/S000368382660034X","DOIUrl":"10.1134/S000368382660034X","url":null,"abstract":"To solve the problems associated with the disposal of plastics, various approaches are being developed aimed at their accelerated destruction. One of the actively developing areas is environmentally friendly bio-utilization using insects. Using physicochemical methods of analysis, the conversion of plastics in the intestinal tract of the insect class has been proven. These include darkling beetles (Coleoptera, Tenebrionidae), pyraloid moths (Lepidoptera, Pyraloidea), and soldier flies (Diptera, Stratiomyidae). The mechanism responsible for the chemical degradation of plastics is considered to be the depolymerization and hydrolysis of plastics due to the enzymatic activity of the microbiota of the insect digestive system. Plastic bioconversion in insects is a complex process that depends on both the symbionts and the host’s intestinal environment. Feeding plastic-degrading insects diets containing different types of plastics results in changes in their microbiota and enrichment in genetically encoded enzymes involved in the breakdown of such polymers. Possible mechanisms of plastic decomposition by insects are considered, including step-by-step physical destruction using the organs of the mouth apparatus, the entry of microparticles into the intestinal tract, depolymerization by oxidation, and hydrolysis by symbiotic bacteria with the participation of the host organism. Isolation of bacteria that decompose plastic, characterization of enzymes involved in its decomposition processes, and modeling the natural living conditions of symbionts are promising approaches for using insect microbiota to dispose of such hard-to-decompose waste.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 3","pages":"429 - 443"},"PeriodicalIF":1.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148394862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0