Applied Biochemistry and Microbiology最新文献_第4页

The Effect of Fermentation by Lactobacilli on the Functional–Technological Properties of Pea Protein Isolates

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605493

I. V. Kravchenko, V. A. Furalyov, E. S. Pshennikova, A. N. Fedorov, V. O. Popov

{"title":"The Effect of Fermentation by Lactobacilli on the Functional–Technological Properties of Pea Protein Isolates","authors":"I. V. Kravchenko, V. A. Furalyov, E. S. Pshennikova, A. N. Fedorov, V. O. Popov","doi":"10.1134/S0003683824605493","DOIUrl":"10.1134/S0003683824605493","url":null,"abstract":"The effect of fermentation with three bacterial preparations, BK-Uglich-K, BK-Uglich-AV, and BK-Uglich-P (Russia), on solubility, emulsifying activity, emulsion stability, foaming, and foam stability of isolates from two pea varieties was studied. The fermentation with bacterial cultures was shown to increase the solubility of isolates by 4 to 17.5 times at pH 3, over 3 times at pH 4, by 23 to 80% at pH 5, by 27 to 43% at pH 6, and by 18 to 27% at pH 7. The fermentation increased the index of emulsifying activity of the isolates at pH 5 by 37% (in one of the varieties). The stability index of the emulsion grew by 19 to 28% at pH 3, by 17 at pH 4, by 18% (in one of the varieties) at pH 5, and by 16 to 35% at pH 6. The fermentation increased the foaming of isolates at pH 3 by 2.2 times, at pH 4 by 1.4 to 2.4 times, at pH 5 and 6 by 1.8 to 4 times, and at pH 7 by 2.1 to 2.4 times. At the same time, the stability of the foam of isolates grew at by 11 to 22% pH 4, by 11 to 13% at pH 5, by 15% (in one of the varieties) at pH 6, and by 28% (in one of the varieties) at pH 7. The results made it possible to select bacterial preparations to improve the parameters of the pea protein isolates intended for the manufacture of various food products, particularly, pea cola (BK-Uglich-P), analogues of fermented milk products and analogues of milk (BK-Uglich-AV).","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1388 - 1397"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Bacterial Sensitivity to a Bacteriophage by Using a Compact Acoustic Analyzer

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605663

O. I. Guliy, B. D. Zaitsev, O. A. Karavaeva, I. A. Borodina

{"title":"Determination of Bacterial Sensitivity to a Bacteriophage by Using a Compact Acoustic Analyzer","authors":"O. I. Guliy, B. D. Zaitsev, O. A. Karavaeva, I. A. Borodina","doi":"10.1134/S0003683824605663","DOIUrl":"10.1134/S0003683824605663","url":null,"abstract":"The discovery of bacteriophages allowed the development of new ways to control the bacterial number and to assess bacterial viability. The most obvious application of bacteriophages is the treatment of human bacterial infections (phage therapy). The advantage of phage therapy is the extreme specificity of phages, since they interact with and infect only certain bacteria without affecting other bacteria or cell lines of other organisms. The advances in phage biology led to the widespread use of specific phage–host interactions in medicine and the agricultural and food industries. Thus, bacteriophages are an alternative to antibiotics for fighting infections and destroying pathogenic bacteria. This work has demonstrated for the first time the potential of a compact acoustic sensor system for assessing the impact of bacteriophages on microbial cells and the bacteriophage sensitivity of the latter. It has been shown that the developed system can be used to evaluate the activity of bacteriophages against microbial cells within 5 min without taking into account the time of cultivation of microbial cells for analysis. The results obtained are promising for further development of the acoustic sensory system in phage therapy.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1398 - 1406"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Chitosan–Caffeic Acid Conjugate and Bacillus subtilis Bacteria on the Protective Reactions in PVY-Infected Plants under Soil Water Deficit

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-12-18 DOI: 10.1134/S0003683824605560

J. N. Kalatskaja, L. G. Yarullina, N. A. Yalouskaya, G. F. Burkhanova, E. I. Rybinskaya, E. A. Zaikina, I. A. Ovchinnikov, V. O. Tsvetkov, K. M. Herasimovich, E. A. Cherepanova, O. A. Ivanov, K. S. Hileuskaya, V. V. Nikalaichuk

{"title":"Effect of Chitosan–Caffeic Acid Conjugate and Bacillus subtilis Bacteria on the Protective Reactions in PVY-Infected Plants under Soil Water Deficit","authors":"J. N. Kalatskaja, L. G. Yarullina, N. A. Yalouskaya, G. F. Burkhanova, E. I. Rybinskaya, E. A. Zaikina, I. A. Ovchinnikov, V. O. Tsvetkov, K. M. Herasimovich, E. A. Cherepanova, O. A. Ivanov, K. S. Hileuskaya, V. V. Nikalaichuk","doi":"10.1134/S0003683824605560","DOIUrl":"10.1134/S0003683824605560","url":null,"abstract":"The effect of chitosan–caffeic acid (Ch–CA) conjugate separately and in combination with a mixture of Bacillus subtilis 47 on the plant defense against PVY under optimal hydration and water deficit in soil was evaluated. The treatments of Ch–CA and Ch–CA+B. subtilis 47 on healthy potato plants under optimal soil moisture conditions demonstrated the accumulation of proline and phenolic compounds, as well as the activation of PPO, which collectively led to an increase in the nonspecific plant defenses. The application of Ch–CA resulted in a reduction of PVY infection in potato plants grown under both optimal and soil moisture-deficient conditions and led to an increase in the potato mini-tuber’s mass. The combination of B. subtilis 47 and Ch–CA proved effective in reducing the infection level exclusively under conditions of soil water deficit. It has been demonstrated that the primary factor influencing the development of resistance in potato plants to PVY under moisture-limiting conditions is associated with an elevated peroxidase activity and alterations in antioxidant activity within plant tissues.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1328 - 1337"},"PeriodicalIF":1.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression, Purification and Partial Characterization of Recombinant S-Adenosyl-L-Homocysteine Hydrolase from Banana

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S000368382460489X

T. Đurašinović, V. Bazović, A. Nešić, A. Ramdan, A. Mahfoud, J. Trbojević-Ivić, M. Gavrović-Jankulović

{"title":"Expression, Purification and Partial Characterization of Recombinant S-Adenosyl-L-Homocysteine Hydrolase from Banana","authors":"T. Đurašinović, V. Bazović, A. Nešić, A. Ramdan, A. Mahfoud, J. Trbojević-Ivić, M. Gavrović-Jankulović","doi":"10.1134/S000368382460489X","DOIUrl":"10.1134/S000368382460489X","url":null,"abstract":"We have recently identified S-adenosyl-L-homocysteine hydrolase (SAHH) as a novel banana allergen with a potentially decisive role in the development of cross-reactivity between plant-derived food and respiratory allergens. The recalcitrant nature of banana pulp and intrinsically low abundance of SAHH limit its production from natural sources. Our objective was to optimize production and biochemically characterize recombinant banana SAHH (rSAHH) with implications for the food safety industry, food allergy diagnosis and treatment, and basic research in molecular allergology. rSAHH with C-terminal 6His tag was successfully expressed in Escherichia coli BL21(DE3) cells, and purified to homogeneity by immobilized metal affinity chromatography (IMAC), yielding 10 mg of rSAHH/L of cell culture under optimized conditions. It is functional as a monomer with an approximate molecular weight of 55 kDa and pI of 5.83. Structural integrity, IgE reactivity, and biological activity of rSAHH were confirmed by Western blot and standard colorimetric SAHH assay with Ellman’s reagent, respectively. As a mesophilic enzyme with wide pH stability and high 8-month storage stability, rSAHH obtained in this study is the promising candidate for further diagnostic and therapeutic applications. To the author’s best knowledge, rSAHH is the only recombinantly produced plant-derived SAHH thus far.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1153 - 1161"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Action, Biological Characteristics, and Future Prospects: A Review of Antimicrobial Peptides (A Review)

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683823603104

X. Ma, Z. Chen, M. Long

{"title":"Mechanisms of Action, Biological Characteristics, and Future Prospects: A Review of Antimicrobial Peptides (A Review)","authors":"X. Ma, Z. Chen, M. Long","doi":"10.1134/S0003683823603104","DOIUrl":"10.1134/S0003683823603104","url":null,"abstract":"Drug-resistant microbes are evolving as a result of the overuse of antibiotics in recent years leading to an increase in the prevalence of drug-resistant infections. Thus, the antibiotics that are currently in development are far from sufficient to treat illnesses and their problems brought on by multi-drug resistance. Antimicrobial peptides (AMPs), which are widely found in the biological world, may help solve this problem. AMPs are highly effective at inhibiting the growth of drug-resistant bacteria via a variety of complex action mechanisms and also have high target specificity. Conjugation to molecules such as antibiotics, proteins, polymers, and nanoparticles are strategies under investigation to boost the therapeutic efficacy of AMPs. As such, they are widely expected to be the basis of a new way of fighting infection in place of antibiotics. This review primarily focuses on these action mechanisms and highlights the biological characteristics and promising applications of AMPs. It also evaluates their prospects and difficulties moving forward. In addition, the antimicrobial efficacies of the design and application of AMPs’ conjugates are summarized to provide a useful reference for future research on AMPs and their application.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1044 - 1060"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecules: Application in RNA-fish Probes

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S000368382360286X

R. Eustáquio, A. T. Caldeira, S. Arantes, A. Candeias, A. Pereira

{"title":"New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecules: Application in RNA-fish Probes","authors":"R. Eustáquio, A. T. Caldeira, S. Arantes, A. Candeias, A. Pereira","doi":"10.1134/S000368382360286X","DOIUrl":"10.1134/S000368382360286X","url":null,"abstract":"Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. These fluorescent labels create chemically stable and minimally disruptive bioconjugates. Amino-reactive fluorescent labels, due to their ease of incorporation into biomolecules, are commonly used in applications like fluorescence in situ hybridization, histochemistry, cell tracing, receptor binding, and immunochemistry. However, the existing popular fluorescent labels are expensive, making coumarin derivatives a potential cost-effective solution for developing bright fluorophores. In this study, the fluorescent 4-styrylcoumarin derivative labels were synthesized and evaluated as potentially effective fluorescent labels for biomolecules. Twelve new fluorescent oligonucleotide probes have been obtained, 6 directed to the rRNA region of eukaryotic cells (EUK516) and 6 directed to the rRNA region of prokaryotic cells (EUB338). The developed fluorescent probes were tested on microorganisms belonging to the culture collection of the Laboratory of Biodegradation and Biotechnology of the HERCULES Laboratory (University of Évora, Portugal), showing effective performance as RNA-FISH probes. These findings evidenced the applicability of the new 4-styrylcoumarin derivatives in labeling of biomolecules and bioimaging.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1118 - 1130"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth of Chlorella vulgaris during Active Immobilisation in Calcium Alginate 小球藻在海藻酸钙活性固定过程中的生长

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683823603062

B. S. de C. Barcellos, M. Gutterres

{"title":"Growth of Chlorella vulgaris during Active Immobilisation in Calcium Alginate","authors":"B. S. de C. Barcellos, M. Gutterres","doi":"10.1134/S0003683823603062","DOIUrl":"10.1134/S0003683823603062","url":null,"abstract":"Microalgae immobilisation is an attractive alternative for wastewater treatment. These microorganisms have high nutrient absorption capability, and when immobilised, are easy to apply and collect into the treatment, although the viability of cells and effectiveness of this process under these conditions still needs to be explored. This study aims to evaluate the viability of Chlorella vulgaris cells taken at 3 initial concentrations, C1, 5.93 × 105 ± 8.84 × 104, C2, 1.75 × 106 ± 5.09 × 105 and C3, 2.95 × 107± 9.81 × 105 cells/mL, immobilised in the calcium alginate beads for the nutrients removal in Jaworski’s modified medium. The cell growth of immobilised and suspended cells was analysed by cell counting and chlorophyll content by fluorescence spectroscopy in an excitation-emission matrix. The results revealed that the immobilised microalgae grew and removed nutrients regardless of initial concentration. The most concentrated immobilised C. vulgaris (C3) had the highest concentration of cells, 1.67 × 108 cells/mL, and removed more nutrients (100% of nitrate and 96.33% of phosphate) with chlorophyll fluorescence of C3, 22.99 RFU at the end of experiment.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1187 - 1195"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of recA Dual Luciferase Reporter System and Its Application on Screening of Dietary Inhibitors for Shiga Toxin-Converting Bacteriophage Induction

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824604190

J. Hu, Y. Wu, L. Kang, Y. Liu, Y. Wu, D. Han, J. Wang

{"title":"Construction of recA Dual Luciferase Reporter System and Its Application on Screening of Dietary Inhibitors for Shiga Toxin-Converting Bacteriophage Induction","authors":"J. Hu, Y. Wu, L. Kang, Y. Liu, Y. Wu, D. Han, J. Wang","doi":"10.1134/S0003683824604190","DOIUrl":"10.1134/S0003683824604190","url":null,"abstract":"Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen that seriously threats the public and animal health. The major virulence of STEC is Shiga toxin (Stx) which is encoded by Stx prophage. Stx prophage induction leads to the production of Stx. Our recent research has shown that dietary functional sugars can differently regulate Shiga toxin type 2 (Stx2) prophage induction. However, high-throughput assays are required to find more dietary inhibitors for Stx prophage induction. This study aims to establish the recA dual-luciferase reporter system for rapidly screening the functional sugars that can inhibit Stx prophage induction. Results showed that the recA dual-luciferase reporter plasmid was successfully constructed as confirmed by DNA sequencing. Established recA dual-luciferase reporter system showed similar screening results when compared with traditional temperate phage determination method (i.e., qPCR) and suggested that L-arabinose is a dietary functional Stx prophage induction inhibitor. Supplementation with L-arabinose in culture medium reduces Stx production in STEC isolated from fecal samples of diarrhea piglets. Collectively, the recA dual-luciferase reporter system is a promising tool for convenient and rapid screening of dietary Stx prophage induction inhibitor, especially suitable for large-scale screening experiments.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1131 - 1136"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Enhancement of Guaiacol Compounds in Sesame-Flavored Baijiu via Inoculation with Pseudomonas azotoformans YF-58 通过接种假单胞菌 YF-58 提高芝麻香型白酒中愈创木酚化合物含量的探索

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824605365

Z. Deng, M. Zhu, Y. Du, Z. Wu, X. Lin, J. Wen, K. Gomi, W. Zhang

{"title":"Exploring the Enhancement of Guaiacol Compounds in Sesame-Flavored Baijiu via Inoculation with Pseudomonas azotoformans YF-58","authors":"Z. Deng, M. Zhu, Y. Du, Z. Wu, X. Lin, J. Wen, K. Gomi, W. Zhang","doi":"10.1134/S0003683824605365","DOIUrl":"10.1134/S0003683824605365","url":null,"abstract":"Guaiacol, 4-vinylguaiacol, 4-methylguaiacol and 4-ethylguaiacol, being guaiacol compounds characterized by spicy and smoky flavor, are important functional factors and key aroma components in sesame flavore Baijiu. In this study, to enhance the content of guaiacol compounds in this product, a strain of Pseudomonas azotoformans YF-58 which produced guaiacol compounds was added to the Zaopei (fermented grains) in the form of bacterial Fuqu. Subsequently, the effect of YF-58 strain on the fermentation system was explored from multiple perspectives, including microbial community composition, volatile flavor substance content and the expression levels of functional enzymes. The results showed that the addition of YF-58 strain altered the relative abundance of microorganisms such as Lactobacillus, Aspergillus, Pseudomonas, Staphylococcus, Weiniella, Bacillus and Corynebacterium in Zaopei. Moreover, the contents of acids, esters, alcohols and guaiacol compounds in Zaopei increased. Additionally, key enzymes associated with guaiacol compounds formation, such as 4-hydroxy-3-polyprenylbenzoate decarboxylase (EC 4.1.1.98), cellulase (EC 3.2.1.4) and catechol O-methyltransferase (EC2.1.1.6), were up-regulated. As a result, the contents of guaiacol, 4-methyl guaiacol, 4-ethylguaiacol and 4-vinylguaiacol in sesame flavor Baijiu were significantly increased by 193.1, 11.6, 95.4 and 105.2%, respectively. The research findings provided some theoretical guidance for enhancing the content of volatile flavor substances in Baijiu.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1363 - 1374"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Lemon Juice Induced Changes in Fatty Acid Composition, Membrane Fluidity and Virulence in Pathogenic Escherichia coli 评估柠檬汁诱导致病性大肠杆菌脂肪酸组成、膜流动性和毒性的变化

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824604050

S. Singha, R. Thomas, J. N. Vishwakarma, D. Bharadwaj, D. Sharma, V. K. Gupta

{"title":"Evaluation of the Lemon Juice Induced Changes in Fatty Acid Composition, Membrane Fluidity and Virulence in Pathogenic Escherichia coli","authors":"S. Singha, R. Thomas, J. N. Vishwakarma, D. Bharadwaj, D. Sharma, V. K. Gupta","doi":"10.1134/S0003683824604050","DOIUrl":"10.1134/S0003683824604050","url":null,"abstract":"Acidification is a crucial preservation technique that affects the survival and infection of foodborne pathogens. In this study, influence of the acidic growth medium on the resistance pattern of the pathogenic Escherichia coli in terms of changes in fatty acid composition as well as expression of virulent genes (eae, stx1, stx2 and hlyA) was investigated. It was revealed that lemon juice mediated acidic pH induced the transcription of stx2, with 3.1- and 2.5-fold increase of survived cells at pH 3.5 and 4.5, respectively, while the transcription level of stx1 in cells grown at pH 3.5 and 4.5 were 0.57 and 0.71-fold lower compared to control. Highest transcription level was observed in the cells grown at pH 3.5 and containing hlyA (4.6-fold), which was much higher than that at pH 5.5 and 4.5. However, pH had no effect on the transcription of eae gene. The results showed that isolated pathogenic E. coli cells from meat were susceptible to lemon juice mediated stress possibly due to the increased level of unsaturated fatty acid (USFA) and decreased level cyclic fatty acid (CFA). The fluidity of the membranes increased as the pH of the media dropped. The highest USFA/SFA ratio was observed in strains incubated at pH 3.5 with ratio of 0.31-0.38 and the value decreased as the pH increased, reaching a minimum value of 0.03 at pH 7.0. This study emphasizes on the importance of establishing effective strategies to combat acid-resistant pathogenic E. coli in food matrices.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1137 - 1146"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0