Applied Biochemistry and Microbiology最新文献_第4页

Obtaining Isotopically Labeled Preparations of Recombinant NAD+-Dependent Formate Dehydrogenase from Pathogen Staphylococcus aureus 从金黄色葡萄球菌中获得重组NAD+依赖性甲酸脱氢酶的同位素标记制剂

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700066

T. S. Iurchenko, A. A. Pometun, D. L. Atroshenko, T. A. Chubar, V. I. Polshakov, S. S. Savin, V. I. Tishkov

{"title":"Obtaining Isotopically Labeled Preparations of Recombinant NAD+-Dependent Formate Dehydrogenase from Pathogen Staphylococcus aureus","authors":"T. S. Iurchenko, A. A. Pometun, D. L. Atroshenko, T. A. Chubar, V. I. Polshakov, S. S. Savin, V. I. Tishkov","doi":"10.1134/S0003683824700066","DOIUrl":"10.1134/S0003683824700066","url":null,"abstract":"NAD+-dependent formate dehydrogenase from Staphylococcus aureus (SauFDH) plays a key role in the biofilm growth of this pathogen. Under these conditions, the reaction catalyzed by SauFDH is the main source of energy in the cell. Therefore, highly effective SauFDH inhibitors can be used as a basis for the development of fundamentally new drugs against S.aureus biofilms. The Nuclear Magnetic Resonance method is one of the most powerful and effective for studying the interaction of promising inhibitors with the enzyme globule. In this work, we optimized the cultivation conditions for the effective introduction of 2H, 13C and 15N isotopes into the enzyme, both individually and together. MALDI/TOF/TOF mass spectra of peptides of isotopically labeled enzyme preparations after tryptic hydrolysis of samples SauFDH 2H, SauFDH 2H,15N,12C and SauFDH 2H,15N,13C showed a uniform distribution of these isotopes in the protein molecule with a degree of enrichment of at least 95% in 2H and not less than 98% for 13C and 15N. This level of isotope enrichment is the maximum achievable in a research laboratory and is completely sufficient for NMR experiments.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 7","pages":"1475 - 1480"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Characterization of Bacillus subtilis PY79 and NCIB 3610 as Potential Producers of Surfactin 枯草芽孢杆菌PY79和NCIB 3610作为表面素潜在生产者的基因组特征

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700145

V. S. Trefilov, V. A. Labanov, M. G. Khrenova, T. V. Panova, V. A. Rodin, V. Y. Savitskaya, E. A. Kubareva, M. I. Zvereva

{"title":"Genomic Characterization of Bacillus subtilis PY79 and NCIB 3610 as Potential Producers of Surfactin","authors":"V. S. Trefilov, V. A. Labanov, M. G. Khrenova, T. V. Panova, V. A. Rodin, V. Y. Savitskaya, E. A. Kubareva, M. I. Zvereva","doi":"10.1134/S0003683824700145","DOIUrl":"10.1134/S0003683824700145","url":null,"abstract":" Surfactin is a powerful surfactant of biological origin with a wide range of potential applications in industry and medicine. The main factor limiting its active utilization is the high cost of production. Due to the complexity of the structure, the chemical synthesis of surfactin is unprofitable, so the main approach to its production is microbiological synthesis. This work demonstrated for the first time the ability of B. subtilis NCIB 3610 to synthesize surfactin. This strain possesses properties lost by most B. subtilis model laboratory strains. The nucleotide sequences of genomes of B. subtilis NCIB 3610 and B. subtilis PY79 strains have been obtained by nanopore sequencing. А comparative analysis of the obtained genomes with genome of B. subtilis 168, genetically modified derivatives of which are patented surfactin producers, has been performed. No mutations affecting surfactin biosynthesis were found in the NCIB 3610 strain, while in the PY79 strain mutations were identified in genes involved in cellular processes competing with biosynthesis of the surfactin. Thus, the results obtained in this work make it possible to consider B. subtilis NCIB 3610 and PY79 as alternative base strains for the genetic engineering design of surfactin superproducers.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1543 - 1550"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Serotyping of Bacillus turingiensis 图灵芽孢杆菌的分子血清分型

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700157

E. V. Patrusheva, L. N. Borshchevskaya, A. A. Kanikovskaya, S. P. Sineoky

引用次数: 0

Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model 以硅酸钙镁陶瓷透华苷为载体的重组BMP-2和重组rS1/9蜘蛛蛋白为支架的杂交植入物：小鼠开颅模型的修复性成骨

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S000368382470008X

A. S. Karyagina, V. G. Bogush, P. A. Orlova, L. I. Davydova, A. V. Zhulina, T. M. Grunina, N. V. Strukova, M. S. Generalova, M. S. Krivozubov, S. E. Cheperegin, A. A. Ramonova, A. V. Gromov

{"title":"Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model","authors":"A. S. Karyagina, V. G. Bogush, P. A. Orlova, L. I. Davydova, A. V. Zhulina, T. M. Grunina, N. V. Strukova, M. S. Generalova, M. S. Krivozubov, S. E. Cheperegin, A. A. Ramonova, A. V. Gromov","doi":"10.1134/S000368382470008X","DOIUrl":"10.1134/S000368382470008X","url":null,"abstract":"Abstract—Calcium–magnesium silicate ceramic diopside is an effective carrier of BMP-2 and, in combination with a bovine demineralized bone matrix as a scaffold, can induce effective reparative osteogenesis on the model of craniotomy in mice. It was of interest to investigate the efficacy of combined usage of BMP-2-loaded diopside with recombinant spidroins for the induction of osteogenesis. For this purpose, porous implants were prepared based on recombinant spidroin rS1/9, into which BMP-2 was introduced either as BMP-2 loaded diopside particles suspended in hyaluronic acid or by adsorption. Neo-osteogenesis with bone marrow formation occurred in both groups with BMP-2, most pronounced in the group with BMP-2 and diopside on the model of critical size defects in the cranium in mice. Thus, the use of 3D scaffolds based on recombinant spidroin with BMP2-loaded diopside particles in hyaluronic acid can lead to effective induction of osteogenesis. The spidroin scaffolds themselves represent a sufficiently effective carrier for BMP-2.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 7","pages":"1493 - 1503"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids as Complementary Medicines for Therapy and Prevention of COVID-19: Part I. Experimental and Clinical Applications 黄酮类化合物作为治疗和预防COVID-19的补充药物：第一部分：实验和临床应用

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700194

Ya. F. Zverev, A. Ya. Rykunova

引用次数: 0

Optimization of the Structure of the Staphylococcus aureus Phage K Endolysin CHAP Domain to Increase Lytic Activity 优化金黄色葡萄球菌噬菌体K型内溶素CHAP结构域以提高裂解活性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI: 10.1134/S0003683824700170

A. M. Amiryan (, ), E. P. Sannikova, A. V. Serkina, I. I. Gubaidullin, N. V. Bulushova, D. G. Kozlov

{"title":"Optimization of the Structure of the Staphylococcus aureus Phage K Endolysin CHAP Domain to Increase Lytic Activity","authors":"A. M. Amiryan \u0000 (, ), E. P. Sannikova, A. V. Serkina, I. I. Gubaidullin, N. V. Bulushova, D. G. Kozlov","doi":"10.1134/S0003683824700170","DOIUrl":"10.1134/S0003683824700170","url":null,"abstract":" In order to increase the lytic activity of the enzyme, structures of the recombinant modified CHAP domain of the phage K endolysin to Staphylococcus aureus have been developed, including (starting from the N-terminus) a sequence of various cationic peptides HB(X) fused with the sequence of the CHAP domain through the GSG4S linker region. Genetic engineering constructs encoding the new HB(X)-CHAP were obtained, which were cloned and expressed in the recipient strain E. coli BL21(DE3). All variants of HB(X)-CHAP, as well as the control variant of the CHAP domain, were isolated and purified using a single technique involving a combination of cation and anion exchange chromatography. The lytic activity of the obtained enzymes was studied by the turbidimetric method using an autoclavable culture of S. aureus. For the two most promising HB(X)-CHAP variants from the point of view of further use, the main physicochemical characteristics are determined. It was shown that the presence of the GSG4S linker site in the structure of the molecule led to at least a twofold increase in the activity in the lysis of S. aureus cells, while the cationic peptides did not have a positive effect on the lytic activity of endolysin against Staphylococcus aureus. The obtained data may allow a rational approach to the issue of choosing an approach to optimizing the structure in the future and will expand the possibilities for designing endolysins in order to create an effective drug.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1565 - 1574"},"PeriodicalIF":1.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Cobalt Tolerance of Saccharomyces cerevisiae by Engineering the Cytoprotection System 通过改造细胞保护系统增强酿酒酵母对钴的耐受性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-01-20 DOI: 10.1134/S0003683824604049

Y. Yang, G. Sun, X. Ge, C. Xia, C. Zhang, Y. Zhu, Z. Wang

引用次数: 0

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-01-20 DOI: 10.1134/S0003683824604840

Natchanok Talapphet, Moon-Moo Kim

{"title":"Tyrosinase-related Protein 1 Gene Knockout by CRISPR/Cas9 System Induces Apoptosis in Human Fibrosarcoma Cells","authors":"Natchanok Talapphet,  Moon-Moo Kim","doi":"10.1134/S0003683824604840","DOIUrl":"10.1134/S0003683824604840","url":null,"abstract":"Tyrosinase-related protein 1 (TRP-1) has been known to play an important role in melanogenesis. While we had studied TRP-1 gene knockout using the CRISPR/Cas9 system, it was found that TRP-1 gene knockout could induce apoptosis of human fibrosarcoma cells (HT1080). The purpose of this study was to investigate the effect of TRP-1 knockout on the induction of apoptosis in HT1080 cells. The TRP-1 KO cells were successfully established using the CRISPR/Cas9 system, and the frameshift mutation was verified by the Sanger DNA sequencing analysis. The modified TRP-1 structure by frameshift mutation caused a decrease in gene and protein expression in TRP-1 KO cells. The TRP-1 KO cells were phenotypically changed and induced apoptosis. The flow cytometry analysis showed the early apoptosis phase. Furthermore, the TRP-1 KO cells decreased the scavenging activity of hydrogen peroxide, compared to normal cells. DNA fragmentation and TUNEL assays confirmed the apoptotic cell death in these cells. The expression levels of proteins such as Caspase 3, Bax, and Bcl-2 were positively modulated in the apoptosis induction of TRP-1 KO cells in a mitochondrial-dependent pathway. The expression levels of Catalase, SOD1, and SOD2 were remarkably decreased in the these cells, compared to normal cells. The expression levels of MAPKs kinase such as p38, ERK1/2, JNK, and NF-κB related to cell proliferation were also reduced in the TRP-1 KO cells. Therefore, our findings suggest that TRP-1 could play an important role in apoptosis in addition to melanin production.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"36 - 48"},"PeriodicalIF":1.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pluramycinone Metabolites from Streptomyces sp. SA0215, Isolated from the Saudi Red Sea Sediments with Antimicrobial and Cytotoxic Activity 从沙特红海沉积物中分离出的链霉菌 SA0215 的 Pluramycinone 代谢物具有抗菌和细胞毒性活性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-01-20 DOI: 10.1134/S0003683824604219

M. Ghandourah

{"title":"Pluramycinone Metabolites from Streptomyces sp. SA0215, Isolated from the Saudi Red Sea Sediments with Antimicrobial and Cytotoxic Activity","authors":"M. Ghandourah","doi":"10.1134/S0003683824604219","DOIUrl":"10.1134/S0003683824604219","url":null,"abstract":"A derived actinomycete, Streptomyces sp. SA0215 was isolated from Saudi Red Sea sediments; the strain was identified by morphology and by phylogenetic analysis of the 16S rRNA gene sequence. The chemical analysis of its culture broth yielded 9 secondary metabolites. The compounds were isolated by a series of chromatographic steps, and their structures established by detailed spectroscopic analysis of the NMR and MS data. Pluramycinone metabolites 1-6 with the structurally related indomycinone analogous as saptomycin-A (1), saptomycin-F (2), α-indomycinone (3), β-indomycinone (4), γ-indomycinone (5), kidamycin (6) and furthermore, resistomycin (7), 1-acetyl-β-carboline (8) and ergosterol peroxide (9) were identified from the culture broth extract. Bioactivity of the indomycinones 1–6 was evaluated in antimicrobial and cytotoxicity assays. β-Indomycinone (4) was found to exhibit potent broad spectrum antibacterial activities against the tested pathogens Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus with inhibition zones diameters of 18, 20 and 24 mm, respectively, compared to the inhibition zones of the antibiotic reference oxytetracycline. The MIC values against the same pathogens were determined 7.2, 11.5, and 13.7 µg/mL, respectively. While γ-indomycinone (5) displayed high activity against the Gram-positive S. aureus with MIC value 10.3 µg/mL, compared to the potent activity of the crude extract (MIC = 9.6 µg/mL). Moreover, saptomycin-A (1) displayed the highest cytotoxic activity against human HepG2 cells with an IC50 value of 19.3 μM, followed by β-indomycinone with an IC50 value of 23.5 μM, while the extract showed the highest activity against human MCF7 cells with an IC50 value of 30.5 µg/mL.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"110 - 116"},"PeriodicalIF":1.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Antioxidative Enzymes: Biotechnological Production and Environmental and Biomedical Applications 微生物抗氧化酶：生物技术生产以及环境和生物医学应用

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2025-01-20 DOI: 10.1134/S0003683824604207

V. M. Songire, R. H. Patil

{"title":"Microbial Antioxidative Enzymes: Biotechnological Production and Environmental and Biomedical Applications","authors":"V. M. Songire, R. H. Patil","doi":"10.1134/S0003683824604207","DOIUrl":"10.1134/S0003683824604207","url":null,"abstract":"Microbial antioxidative enzymes play a crucial role environmental and biomedical application. The key environmental applications of microbial antioxidative enzymes include addressing challenges related to pollution, waste management, and environmental degradation. The main antioxidant enzymes involved in converting hazardous wastes into nontoxic or biodegradable materials include catalases, superoxide dismutases, xanthine oxidases, and glutathione peroxidases. In living cells, antioxidative enzymes cooperate to shield cells from excess reactive oxygen species derived from endogenous metabolism or the external microenvironment and eliminate oxidative stress. These enzymes are crucial to the pharmaceutical, cosmetic, and food industries as well as in the treatment of a number of diseases. Biotechnological production of these enzymes involves both solid-state and submerged fermentation processes. Solid-state fermentation is gaining popularity over submerged processes because it is economical and environmentally benign. The bioprocessing for the production of antioxidative enzymes involves diverse microorganisms. The enzymes are purified by chromatography methods such as gel filtration, gas chromatography, ion exchange and HPLC. The present study focused on microbial antioxidative enzyme production and its applications. This review comprehensively covers the diversity of antioxidative enzyme-producing microorganisms, the biotechnological production of antioxidative enzymes, the bioprocessing parameters and the applications of antioxidant enzymes in various fields.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"61 1","pages":"1 - 26"},"PeriodicalIF":1.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0