Applied Biochemistry and Microbiology最新文献_第5页

Two Immunochromatographic Assays Based on Hematite (α-Fe2O3) Nanoparticles for Detection of Aflatoxin M1 and Escherichia coli O157:H7 in the Milk

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824603111

Y. B. Zhu, W. Zhang, Y. L. Fan, Y. Y. Chen, J. L. Zhu, H. X. Lu

引用次数: 0

Nutritional Profile and Antibacterial Activity of the Black Soldier Fly (Hermetia illucens) Larvae Meal as Potential Protein Source for Aquafeeds

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824604773

C. M. Geronda, C. H. Choresca, F. L. Pedroso, C. M. Caipang, J. W. Repamonte, F. F. Fagutao

{"title":"Nutritional Profile and Antibacterial Activity of the Black Soldier Fly (Hermetia illucens) Larvae Meal as Potential Protein Source for Aquafeeds","authors":"C. M. Geronda, C. H. Choresca, F. L. Pedroso, C. M. Caipang, J. W. Repamonte, F. F. Fagutao","doi":"10.1134/S0003683824604773","DOIUrl":"10.1134/S0003683824604773","url":null,"abstract":"The black soldier fly (BSF, Hermetia illucens) is a well-known insect with promising potential in transforming organic wastes into protein-rich biomass. The BSF larvae (BSFL) can replace expensive sources of protein used in aquaculture and have antimicrobial characteristics that are recognized to inhibit pathogenic bacteria in humans, plants, poultry, and livestock. However, its potential against pathogenic bacteria in aquaculture remains largely unexplored. To address this gap, BSFL in different instar stages were analyzed for their proximate, mineral, and fatty acid contents to determine their nutritional composition. Additionally, disk diffusion and minimum inhibitory concentration assays were used to evaluate the antibacterial activity of the crude methanolic extracts of the BSFL against Aeromonas veronii, Edwardsiella tarda, Streptococcus agalactiae, and Vibrio harveyi. Results showed high nutritional composition across instar stages but the highest level was revealed in 2- and 3-instar stages (crude protein: 36.17, 36.96%, and crude fat: 33.84, 40.90%, respectively). Crude methanolic extract of BSFL inhibited the growth and proliferation of all tested bacteria, with the highest inhibition observed in Streptococcus agalactiae. Moreover, the extracts obtained at 5-instar exhibited the highest antibacterial activity compared to other extracts. This study showed that the BSFL have the potential to serve as a sustainable protein source in aquafeeds, and the crude methanolic extracts from later-stage BSFL can be used as antibacterial agents in aquaculture.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1375 - 1387"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Growth-Promoting Microbes for Sustainable Crop Production (A Review) 促进植物生长的微生物促进可持续作物生产（综述）

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683823603116

Jyotirmayee Das, Madhusmita Panigrahy, Shraddha Mohanty, Bandita Jena, R. K. Nayak, A. K. Shukla

{"title":"Plant Growth-Promoting Microbes for Sustainable Crop Production (A Review)","authors":"Jyotirmayee Das, Madhusmita Panigrahy, Shraddha Mohanty, Bandita Jena, R. K. Nayak, A. K. Shukla","doi":"10.1134/S0003683823603116","DOIUrl":"10.1134/S0003683823603116","url":null,"abstract":"Plant growth-promoting microbes (PGPM) are a group of beneficial microorganisms that stimulate plant growth directly by improving nitrogen fixation, phosphate solubilization, mineral uptake/transport, plant growth hormone/regulator metabolism, and indirectly by systemic induced/acquired resistance by salicylic/jasmonic acid production. These microorganisms can act against plant pathogens by exoenzymes, secondary metabolite production, osmolyte production in abiotic stress. Pseudomonas/Bacillus spp. are the most versatile plant growth-promoting bacteria (PGPB). The PGPMs can be used as rhizome-remediators, phytostimulators, biofertilizers, and stress controllers. The PGPMs’ treatment promotes the growth of several plants such as lentils, peas, cucumbers, and soybeans. Their availability depends largely on interactions between microorganisms in soil and the ecto- and endophytes of root, stem, and leaf. PGPMs affect positively to plant growth, nutrient uptake, biotic resistance, and disease-causing pathogens, and contributes to an increase in yield in several crop plants. This review extensively covers all these aspects of various ways adapted by the PGPM to stimulate positively plant growth effects. PGPB influence phosphorus (P) homeostasis to induce beneficial effects in plants. The review introduces the topic from the perspectives of P and their relation with PGPB. It interlinks the entomopathogens and endophytes with the PGPBs’ regulation of plant growth. Different mechanisms and techniques of P solubilization by the PGPB are discussed. Followingly, the genetics and metagenomics of P solubilization are explained. This review opens various perspective points for further research for enhancing plant growth, strengthening sustainable agriculture of food crops.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1282 - 1291"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Organic Solvent Tolerant NAD+-dependent Formate Dehydrogenase from Halophilic Yeast Candida diddensiae 来自嗜卤酵母菌 Candida diddensiae 的新型耐有机溶剂 NAD+依赖性甲酸脱氢酶

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S000368382460012X

G. Kurt-Gür, E. Arslan, Ö. Başsaraç, E. Ordu

{"title":"A Novel Organic Solvent Tolerant NAD+-dependent Formate Dehydrogenase from Halophilic Yeast Candida diddensiae","authors":"G. Kurt-Gür, E. Arslan, Ö. Başsaraç, E. Ordu","doi":"10.1134/S000368382460012X","DOIUrl":"10.1134/S000368382460012X","url":null,"abstract":"Characterization of new halophilic enzymes capable of thriving in non-aqueous biocatalysis of significant interest in the non-aqueous chemoenzymatic processes. Despite the number of studies dealing with increasing the stability of NAD+-dependent formate dehydrogenases (FDHs), there are few studies characterizing their behavior in organic solvents. FDHs have a great advantage in cofactor regeneration systems and the reduction of CO2 to formate. It is important to find novel FDHs for the wide application field of organic chemicals. In this study, a novel NAD+-dependent formate dehydrogenase from Candida diddensiae YLP9 (CdFDH), isolated from olive brine and having tolerance under low pH and high salt concentration, was heterologously expressed and characterized. Recombinant CdFDH has optimum activity at pH 7.0–8.0 when the substrate is formate. The midpoint of thermal inactivation (T0.5) for CdFDH was calculated as 54°C. The enzyme shows high stability at рН 6.0 and 53°С ((k_{{{text{in}}}}^{{{text{ef}}}}) 0.28 × 10–4 /s). Comparing the enzyme activity results obtained from incubation of enzyme in media without salt and with different concentrations of NaCl, KCl, and MgCl2 revealed that concentrations of NaCl and KCl between 0.4–4.0 M contribute to proper folding and activity of CdFDH. Enzyme activity was preserved and significantly increased after incubating in the 20–80% of DMSO, 40% 3-methyl butanol, 70% n-butanol and, all tested concentrations of n-hexane. The results show that NAD+-dependent CdFDH is a promising candidate biocatalyst to use in non- or low-aqueous biotechnological applications.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1162 - 1170"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Antimicrobial Activity in Fungi-destructors of Tempera Painting Isolated in the State Tretyakov Gallery 测定国立特列季亚科夫美术馆分离出的钢笔画真菌破坏者的抗菌活性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-10-01 DOI: 10.1134/S0003683824605742

A. A. Ermolyuk, D. A. Avdanina, F. S. Koblov, S. G. Kalinin, T. V. Kolganova, B. F. Vasilieva, M. V. Demiankova, O. V. Efremenkova, A. A. Zghun

{"title":"Determination of Antimicrobial Activity in Fungi-destructors of Tempera Painting Isolated in the State Tretyakov Gallery","authors":"A. A. Ermolyuk, D. A. Avdanina, F. S. Koblov, S. G. Kalinin, T. V. Kolganova, B. F. Vasilieva, M. V. Demiankova, O. V. Efremenkova, A. A. Zghun","doi":"10.1134/S0003683824605742","DOIUrl":"10.1134/S0003683824605742","url":null,"abstract":"In current work, we for the first time studied the antimicrobial activity of representatives of mold fungi, which occupy a rather specific ecological niche, since they are destructors of tempera painting materials. We screened 24 molds that had previously been isolated from exhibits and in the halls of the State Tretyakov Gallery and for which the ability to destroy materials used in tempera painting and scientific restoration was shown. Screening for antimicrobial activity was carried out against a panel of 16 test cultures, characteristic for identifying activities of various types. The strains exhibited activity, including against Staphylococcus aureus INA 00761 (MRSA), S. aureus FDA 209P (MSSA), Pseudomonas aeruginosa ATCC 27853, Mycobacterium smegmatis VKPM Ac-1339, M. smegmatis mc2 155 and Leuconostoc mesenteroides VKPM B-4177. Among the studied paint-degrading fungi, strains Simplicillium lamellicola STG-96, Cerarobasidium sp. STG-324, Penicillium sp. STG-327 and Penicillium sp. STG-344 turned out to be the most promising for further research related to the determination what specific highly active compounds they produce.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1266 - 1274"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of Recombinant Endolysin LysSi3 to Increase its Antibacterial Properties 对重组内溶解素 LysSi3 进行工程改造以增强其抗菌特性

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S000368382460492X

N. P. Antonova, I. V. Grigoriev, A. M. Lendel, O. V. Usacheva, A. A. Klimova, E. V. Usachev, V. A. Gushchin, D. V. Vasina

引用次数: 0

Development of a Bio-Selecting Agent Based on Immobilized Bacterial Cells with Amidase Activity for Bio-Detection of Acrylamide 开发基于具有酰胺酶活性的固定化细菌细胞的生物选择剂，用于生物检测丙烯酰胺

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824605031

E. M. Protasova, Yu. G. Maksimova

{"title":"Development of a Bio-Selecting Agent Based on Immobilized Bacterial Cells with Amidase Activity for Bio-Detection of Acrylamide","authors":"E. M. Protasova, Yu. G. Maksimova","doi":"10.1134/S0003683824605031","DOIUrl":"10.1134/S0003683824605031","url":null,"abstract":"Actinobacteria cells Rhodococcus erythropolis 4-1 and Rhodococcus erythropolis 11-2 and Proteobacteria Alcaligenes faecalis 2, which have amidase activity, were immobilized by entrapping barium alginate and agarose into the gel structure, as well as by obtaining biofilms on thermally expanded graphite (TEG). The operational stability of such immobilized biocatalysts after storage in frozen and dehydrated form was determined, and a prototype of a conductometric acrylamide biosensor based on such a bioselective agent was developed. The most preferred method for storing immobilized cells was freezing at temperatures from –20 to –80°C; long-term storage is also possible wet at 4–25°C. It was shown that these cells were most preferable for the biodetection of acrylamide A. faecalis 2, immobilized in an agarose gel structure. An agarose gel with bacterial cells immobilized in its structure had greater mechanical strength and stability during successive cycles of conversion of acrylamide into acrylic acid compared to barium alginate gel. The mechanical strength of barium alginate gel can be enhanced by the addition of carbon nanomaterials during cell immobilization. Growing biofilms on carbon materials used for manufacturing electrodes is also promising. Biofilms of R. erythropolis 11-2 on TEG are capable of converting acrylamide into acrylic acid in more than 20 reaction cycles while maintaining at least 50% amidase activity.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"942 - 949"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683824605031.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABA-Degrading Strains of Bacteria of the Genus Pseudomonas and Their Influence on Wheat Growth 假单胞菌属细菌的 ABA 降解菌株及其对小麦生长的影响

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604566

A. S. Ryabova, L. Yu. Kuzmina, N. F. Galimsyanova, E. A. Gilvanova, L. B. Vysotskaya, E. V. Martynenko, G. R. Kudoyarova

{"title":"ABA-Degrading Strains of Bacteria of the Genus Pseudomonas and Their Influence on Wheat Growth","authors":"A. S. Ryabova, L. Yu. Kuzmina, N. F. Galimsyanova, E. A. Gilvanova, L. B. Vysotskaya, E. V. Martynenko, G. R. Kudoyarova","doi":"10.1134/S0003683824604566","DOIUrl":"10.1134/S0003683824604566","url":null,"abstract":"Three new representatives of the genus Pseudomonas have been identified that are capable of utilizing abscisic acid and positively influencing the growth and development of plants. Their physiological and biochemical properties have been studied. Based on analysis of the 16S rRNA gene, they were identified as P. veronii IB K11-1 (99.86% similarity) and P. frederiksbergensis IB Ta10m (100%), and strain TaE2 was assigned to Pseudomonas sp. It was found that bacteria, when grown on a mineral-salt medium with ABA, reduced the hormone content by 50–60% with an increase in population density by two orders of magnitude. In a laboratory experiment, it was shown that the introduction of the bacterial biomass (108 CFU/g of substrate) into the rhizosphere of wheat plants ten days after treatment led to a decrease in the abscisic acid content in the roots by 18–30% and an increase in plant weight by up to 30%. Thus, new strains of growth-stimulating ABA-degrading bacteria have been identified and characterized for the first time, which may be promising for the creation of biological products that increase plant resistance to biotic and abiotic stresses.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"925 - 930"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains (Review) 支原体：细胞培养物和病毒株的特性、检测和净化方法（综述）

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604943

O. A. Leonovich

{"title":"Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains (Review)","authors":"O. A. Leonovich","doi":"10.1134/S0003683824604943","DOIUrl":"10.1134/S0003683824604943","url":null,"abstract":"Mycoplasma contamination of continuous cell cultures and collection viral strains remains a serious problem in the biotechnology industry and experimental research. The frequency of mycoplasma contamination of cultured cell lines and viruses is 15–35%, and in some cases up to 80%. Mycoplasmas cause various changes in cultures contaminated by them, including cell death. They have immunomodulatory properties and affect the yield of certain viruses propagated in cell cultures. Mycoplasmas do not have a cell wall, are able to pass through a bacterial filter, have the smallest genome (≈580 kb) among bacteria, and are capable of independent reproduction and existence. These microorganisms are resistant to most antibiotics commonly used in cell culture. Derivative groups of tetracyclines and fluoroquinolones (BM-Cyclin®, Ciprobay®, Baytril®, Plasmocin®, MRA) have shown certain effectiveness in decontaminating viral strains and cell cultures from mycoplasmas. Timely, highly sensitive detection and prevention of mycoplasma infection is of great importance. For routine scanning of mycoplasma infection of continuous cell cultures and viral strains, the methods of indicator cell culture (cytochemical) and polymerase chain reaction (PCR) are recommended, or for more accuracy, microbiological analysis of mycoplasma colonies on a special medium.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"785 - 793"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for Preprocessing Reeds to Obtain Enzymatic Hydrolysates with a High Sugar Content 预处理芦苇以获得含糖量高的酶水解物的方法

IF 1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI: 10.1134/S0003683824604736

M. V. Semenova, A. M. Rozhkova, D. O. Osipov, V. D. Telitsin, E. A. Rubtsova, E. G. Kondrat’eva, I. S. Vasil’eva, O. V. Morozova, A. I. Yaropolov, A. P. Sinitsyn

{"title":"Methods for Preprocessing Reeds to Obtain Enzymatic Hydrolysates with a High Sugar Content","authors":"M. V. Semenova, A. M. Rozhkova, D. O. Osipov, V. D. Telitsin, E. A. Rubtsova, E. G. Kondrat’eva, I. S. Vasil’eva, O. V. Morozova, A. I. Yaropolov, A. P. Sinitsyn","doi":"10.1134/S0003683824604736","DOIUrl":"10.1134/S0003683824604736","url":null,"abstract":"Methods of the pretreatment of reeds (grinding, hydrothermal treatment, treatment with acid or alkali solutions, organosolv, deep eutectic solvents) and their effect on the subsequent enzymatic hydrolysis by cellulase and hemicellulase complexes have been studied. Substrates with the highest reactivity were obtained by exposing the reeds to a weakly alkaline deep eutectic solvent (DES) and an alkali solution. The depth of enzymatic hydrolysis of these pretreated substrates was 63 and 59%, and the degree of conversion of polysaccharides (cellulose and hemicellulose) into monosaccharides taking into account the yield of the substrate at the preprocessing stage was 60 and 34%, respectively. After pretreatment of the reeds with acid DES, water steam, water, or a water/organic solution of sulfuric acid, the depth of enzymatic hydrolysis was 45, 25, 20, and 11% and the degree of polysaccharide conversion was 26, 18, 13, and 10%, respectively. The industrial enzyme preparation Agrocell Plus with a predominant content of cellobiohydrolases and endoglucanases was most effective in hydrolyzing the dust fraction of the reed, as was the reed pretreated with a solution of sulfuric acid or DES (acidic or alkaline). The industrial enzyme preparation Agroxil Plus, containing endoxylanase and cellobiohydrolases, was more effective in hydrolyzing the reed after hydrothermal pretreatment or alkali solution. The results of the hydrolysis of the reed pretreated with acidic or weakly alkaline DES under the action of individual (homogeneous) cellulases indicate that in both cases the key enzyme was cellobiohydrolase 1.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"931 - 941"},"PeriodicalIF":1.0,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0