Applied Biochemistry and Microbiology最新文献

{"title":"Erratum to: Identification of Luteolin and Some Other Phenolics of Propolis as HIV-1-reverse Transcriptase Inhibitors","authors":"F. Ay, H. İ. Guler, A. O. Belduz, M. Sahinkaya, O. Yıldız, S. Kolayli, O. Demircan","doi":"10.1134/S0003683826010011","DOIUrl":"10.1134/S0003683826010011","url":null,"abstract":"","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"400 - 400"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Antibacterial Activity of Biopolymer Films Based on Chitosan of Various Molecular Weights","authors":"V. V. Shchelkova,&nbsp;N. R. Kildeeva,&nbsp;I. A. Vasilenko","doi":"10.1134/S0003683825602847","DOIUrl":"10.1134/S0003683825602847","url":null,"abstract":"<p>In the context of growing antibiotic resistance, the possibility of creating non-toxic and biodegradable film coatings based on chitosan with modulated antibacterial properties is highly relevant. We have analyzed 270 samples of biopolymer films based on chitosan cross-linked with genipin with molecular weights of 60, 190, and 320 kDa. Suspensions of 30 clinical strains of <i>Staphilococcus aureus</i> (<i>n</i> = 10), <i>Escherichia coli</i> (<i>n</i> = 10), and <i>Candida albicans</i> (<i>n</i> = 10) microorganisms at a concentration of 10³ CFU/mL have been used to evaluate the antibacterial activity of the biopolymer chitosan films (CF). Depending on the molecular weight of chitosan and the contact time of the biopolymer films with <i>S. aureus, E. coli, and C. albicans</i>, negative dynamics in the number of viable colonies have been revealed. These results suggest that chitosan-based biopolymers hold promise for the development of medical tools with programmable antibacterial and antifungal properties for practical medical use.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"220 - 226"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obtaining and Study of Polyphenol and Chitosan Complexes","authors":"L. V. Shults,&nbsp;A. A. Krasnoshtanova","doi":"10.1134/S0003683825601787","DOIUrl":"10.1134/S0003683825601787","url":null,"abstract":"<p>The insoluble complexes formed by polyphenols from plant extracts and chitosan are of interest as potential delivery systems for polyphenols in the intestine. The complexes formed by polyphenols from an aqueous sage extract and chitosan have been investigated. These complexes have been shown to be stable in neutral media and to release polyphenols in highly acidic and highly alkaline environments. The complexes demonstrated significant polyphenol release in simulated intestinal fluid, with minimal release in simulated gastric and salivary fluids. It has been demonstrated that the complexes formed with chitosan are primarily composed of flavonoid glycosides and phenolic acids, with flavonoid aglycones present in trace amounts.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"227 - 232"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-Based Enzymatic Cell Wall Lysis Modulator for Bacterial DNA Isolation","authors":"K. Jasem,&nbsp;R. Z. Khayrullin,&nbsp;S. N. Kulikov","doi":"10.1134/S0003683825604573","DOIUrl":"10.1134/S0003683825604573","url":null,"abstract":"<p>The work has demonstrated that high-molecular chitosan enhances the lytic effect of lysostaphin on <i>Staphylococcus aureus</i> cells. More efficient lysis of bacterial cell walls by lysostaphin in the presence of chitosan made it possible to extract larger amounts of high molecular weight DNA suitable for sequencing from bacterial cells.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"318 - 322"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Chitosan Preparations on Spring Bread Wheat Development and Resistance to Diseases Caused by Biotrophic and Hemibiotrophic Fungi under Epidemic Conditions","authors":"L. Ya. Plotnikova,&nbsp;V. V. Knaub,&nbsp;V. V. Fomenko","doi":"10.1134/S0003683825604457","DOIUrl":"10.1134/S0003683825604457","url":null,"abstract":"<p>A comparative study of the effect of linear chitosan (Сh) and the globular form of chitosan, novochizol (Nc), on the growth and development of spring bread wheat, as well as protection against a complex of fungal diseases under epidemic conditions, was carried out. The indicators “Area Under the Disease Progress Curve” (AUDPC) and “Resistance Index” (RI) were calculated based on the observations. The research results showed that the Ch had greater growth-stimulating activity (on seed germination, stem and ear formation, and seed setting) and a protective effect against biotrophic and hemibiotrophic pathogens than Nc. The Nc–SA preparation (complex with 0.01% salisylic acid) showed the greatest protective effect, which increased in relation to the following diseases: leaf rust, stem rust, Septoria tritici blotch, and Septoria nodorum blotch. The increase in grain yield in the Ch-treated variants was mainly due to the growth-stimulating effects, while the Nc–SA treatment provided better protection against a range of diseases caused by the fungi with different feeding types.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"276 - 286"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Kojic Acid, Chitosan, and Their Combinations with Bacillus subtilis Strains on the Inducing Activity of Bacillus subtilis Strains","authors":"I. L. Krasnobaeva,&nbsp;I. I. Novikova,&nbsp;E. V. Popova,&nbsp;N. M. Kovalenko","doi":"10.1134/S0003683825602434","DOIUrl":"10.1134/S0003683825602434","url":null,"abstract":"<p>This study examines the effects of kojic acid, chitosan, and their combinations with <i>Bacillus subtilis</i> strains on wheat resistance to dark brown spot. A concentration-dependent immunomodulatory effect of the acid on wheat resistance to <i>Bipolaris sorokiniana</i> was identified: <i>B. subtilis</i> VKM B-2604D and <i>B. subtilis</i> VKM B-2605D strains demonstrated high efficacy in protecting wheat from dark brown spot, reducing plant infestation by 50% compared to the control (80% infestation). Inclusion of kojic acid in the culture fluid of the strains at a concentration of 1.0% increased twice their immunomodulatory activity. It was established that the following compositions possessed the highest inducing activity<i>: B. subtilis</i> VKM B-2604D + <i>B. subtilis</i> VKM B-2605D CF + chitosan 0.1% + 0.5% kojic acid and <i>B. subtilis</i> VKM B 2604D + <i>B. subtilis</i> VKM B 2605D CF + chitosan 0.1% + 1.0% kojic acid. The biological activity of these compositions was 3 and 6 times higher than the effect of the original strains.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"287 - 291"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Chitosan and Glyoxal Application for Pre-Sowing Treatment of Soft Wheat Seeds in the North-Western Region of the Russian Federation","authors":"M. V. Uspenskaya,&nbsp;M. I. Fokina,&nbsp;L. E. Kolesnikov,&nbsp;A. D. Fateev,&nbsp;Yu. R. Kolesnikova,&nbsp;D. Yu. Radishevskiy","doi":"10.1134/S0003683825601994","DOIUrl":"10.1134/S0003683825601994","url":null,"abstract":"<p>The effect of pre-sowing treatment of soft wheat seeds of the Darya (K-64432) and Leningradskaya 6 (K-64902) varieties using biologically active chitosan with or without the crosslinking agent glyoxal has been evaluated. Two methods of glyoxal administration have been considered: after the treatment with chitosan and simultaneously with chitosan. The phytometric characteristics of the crops, as well as the phytopathological and other indicators of disease development, have been analyzed. Regardless of the processing method, wheat productivity has been shown to increase significantly (by 188%), with the optimal chitosan solution concentrations being 5% for the Darya variety and 6–7% for the Leningradskaya 6 variety. Sequential treatment with chitosan and glyoxal has been found to be the most effective method, particularly reducing the incidence of powdery mildew in the Leningradskaya 6 variety (BE = 95.7%).</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"310 - 317"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological Properties of Glycerohydrogels Based on a Mixture of Chitosan Hydrochloride with Glucomannan, Formed In Situ on the Wound Surface","authors":"O. N. Malinkina,&nbsp;D. V. Pridankina,&nbsp;A. S. Astankova,&nbsp;A. B. Shipovskaya","doi":"10.1134/S0003683825602057","DOIUrl":"10.1134/S0003683825602057","url":null,"abstract":"<p>Hybrid hydrogels formed in situ on the wound surface are considered promising materials for reconstruction and therapy. This paper examines the dermo- ex vivo and mucoadhesive properties in vitro, as well as the antibacterial, hemostatic, and wound-healing activity in vitro and in vivo of glycerohydrogels based on chitosan hydrochloride, glucomannan, silicon tetraglycerolate (a sol-gel precursor), and functional additives. When applied to an intact or wounded dermal surface, the gel-forming composition results in the formation of a protective glycerohydrogel coating (gel film) with explicit adhesive and sorption properties forms in situ within 3–5 minutes. This coating exhibits bacteriostatic and haemostatic action, as well as wound-healing activity. In vivo evaluation of the wound-healing effect showed a statistically significant reduction in skin restoration time compared to the control, allowing us to recommend glycerohydrogel films as effective bioresorbable wound dressings.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"240 - 246"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Bacillus subtilis and Trichoderma asperellum T-36 Strains and Chitosan Salicylate in Soft Wheat Cultivation","authors":"I. I. Novikova,&nbsp;E. V. Popova,&nbsp;L. E. Kolesnikov,&nbsp;I. L. Krasnobaeva,&nbsp;Yu. R. Kolesnikova,&nbsp;L. A. Higherovich,&nbsp;D. Yu. Radishevskiy,&nbsp;M. V. Shevtsov","doi":"10.1134/S0003683825602410","DOIUrl":"10.1134/S0003683825602410","url":null,"abstract":"<p>New data were obtained on the effectiveness of innovative plant protection and growth regulation products based on selected <i>Bacillus subtilis</i> and <i>Trichoderma asperellum</i> T-36 strains and a plant resistance inducer, 0.1% chitosan salicylate, in the cultivation of common wheat (variety Leningradskaya 6, K-64 900). The design of the experiment included treating wheat seeds with the products before sowing and spraying the plants during the growing season. The highest growth-stimulating and protective effect was demonstrated by the polyfunctional complex Kartofin, CL + 0.1% chitosan salicylate (<i>B. subtilis</i> I-5 + 0.1% chitosan salicylate) and Kartofin, CL (<i>B. subtilis</i> I-5, composition), the use of which promoted increased plant productivity by 92.1% (from 0.4 to 0.8 g/plant) and 51.3% (from 0.4 to 0.6 g/plant), respectively. A significant reduction in the infestation of common wheat by powdery mildew and septoria pathogens was noted with their use. The biological efficiency (BE) of the complex Kartofin CL + 0.1% chitosan salicylate (<i>B. subtilis</i> I-5 + 0.1% chitosan salicylate) in relation to the specified diseases was 69.8 and 74.3%, while that of Kartofin CL (<i>B. subtilis</i> I-5, composition) was 95.4 and 74.2%, respectively. A significant decrease in the development of yellow rust was revealed in the experimental variants: Vitaplan SP (BE = 55.5%); 0.1% chitosan salicylate (BE = 56.6%); Kartofin CL (<i>B. subtilis</i> I-5) (BE = 64.1%); and <i>T. asperllum</i> T-36 CL 1 : 10 (BE = 54.8%). A significant reduction in root rot development (by 15.5%) was observed in the experimental variants Kartofin CL (<i>B. subtilis</i> I-5) and <i>T. asperllum</i> T-36 CL 1 : 100. The grayed-out values may also be removed as less significant. The biopreparations studied can be used to develop environmentally friendly wheat cultivation technologies, improve grain quality, and optimize the phytosanitary condition of crops after the development of biopreparation formulations.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"292 - 302"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan and Its Derivatives: Applied Aspects","authors":"T. A. Zhilkina,&nbsp;A. M. Kamionskaya,&nbsp;V. P. Varlamov,&nbsp;I. V. Yakovleva","doi":"10.1134/S0003683825604718","DOIUrl":"10.1134/S0003683825604718","url":null,"abstract":"<p>This review presents a summary of the diverse range of chitosan applications that have been developed recently: from biomedicine, agrobiotechnology, biostimulation, and bioprotection to climate change adaptation, among others. The focus is on the functionalization and controlled optimization of key biopolymer parameters: degree of polymerization, degree of acetylation, distribution of acetylated blocks within the biopolymer chain, density, and cross-linking reagents. The development of chitosan-based systems for encapsulating drugs and vaccines described in this review improves precision drug delivery. The biologization of agriculture is reflected in this review through studies devoted to the use of chitosan as a protective agent against abiotic stresses, such as drought, high and low temperatures, and soil salinization. By focusing on new strategies for the combined use of chitosan and plant-associated soil bacteria, the use of chitosan nanoparticles demonstrates the expanding scope of biopolymer applications in the agricultural sector. The presented discussion of the mechanism of chitosan action on the immune system of plants provides a deeper understanding of how to achieve the targeted effects of bioprotection and biostimulation.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 2","pages":"191 - 197"},"PeriodicalIF":1.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147682687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}