The ABC Transport Protein PotC Plays a Crucial Role in Antibiotic Resistance in Escherichia coli

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2025-01-20 DOI:10.1134/S0003683824603706

Q. Zhao, Y. Wu, J. Sun, J. Zhang, X. Li, X. Pang, S. Gu

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引用次数: 0

Abstract

The phenomenon of microbial resistance is becoming increasingly serious. The efflux pump system plays a key role in bacterial resistance to antibiotics. The transcriptional profile of Escherichia coli treated with antibiotics revealed the pathways and response mechanisms of up- or down-regulation of efflux pump-related genes. In this study, 104 of all 2548 genes, efflux pump-related differentially significantly expressed genes were screened. The most significant spermidine transport in the Gene Ontology functional enrichment analysis was the most likely important pathway for the E. coli species to respond to antibiotics. The functional enrichment results of Kyoto Encyclopedia of Genes and Genomes showed that most of the antibiotic-induced transport proteins belong to the “ABC family efflux pumps”. The results of the protein-protein interaction network indicated that potC might act as a key gene for E. coli to respond to antibiotics. Finally, overexpressing strains of potC were constructed, and the survival rates of overexpressing strains of potC were improved by 18.9, 14.0, 43.3, and 31.7% after treatment with four antibiotics: gentamicin, norfloxacin, chloramphenicol, and cefotaxime sodium, respectively. This further suggested the role of PotC in the resistance of E. coli to antibiotics, providing scientific support for the study of bacterial resistance mechanisms.

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ABC转运蛋白poc在大肠杆菌抗生素耐药性中起关键作用

微生物耐药现象日益严重。外排泵系统在细菌对抗生素的耐药性中起着关键作用。抗生素处理大肠杆菌的转录谱揭示了外排泵相关基因上调或下调的途径和应答机制。本研究从2548个外排泵相关基因中筛选出104个差异显著表达基因。基因本体功能富集分析中最显著的亚精胺转运是大肠杆菌对抗生素反应最可能的重要途径。京都基因与基因组百科全书的功能富集结果表明，抗生素诱导的转运蛋白大部分属于“ABC家族外排泵”。蛋白-蛋白相互作用网络的结果表明，poc可能是大肠杆菌对抗生素产生应答的关键基因。最后构建了过表达菌株，经庆大霉素、诺氟沙星、氯霉素和头孢噻肟钠4种抗生素治疗后，过表达菌株的存活率分别提高了18.9%、14.0%、43.3%和31.7%。这进一步提示了PotC在大肠杆菌对抗生素耐药中的作用，为研究细菌耐药机制提供了科学依据。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.