Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
A. S. Karyagina, V. G. Bogush, P. A. Orlova, L. I. Davydova, A. V. Zhulina, T. M. Grunina, N. V. Strukova, M. S. Generalova, M. S. Krivozubov, S. E. Cheperegin, A. A. Ramonova, A. V. Gromov
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Abstract—Calcium–magnesium silicate ceramic diopside is an effective carrier of BMP-2 and, in combination with a bovine demineralized bone matrix as a scaffold, can induce effective reparative osteogenesis on the model of craniotomy in mice. It was of interest to investigate the efficacy of combined usage of BMP-2-loaded diopside with recombinant spidroins for the induction of osteogenesis. For this purpose, porous implants were prepared based on recombinant spidroin rS1/9, into which BMP-2 was introduced either as BMP-2 loaded diopside particles suspended in hyaluronic acid or by adsorption. Neo-osteogenesis with bone marrow formation occurred in both groups with BMP-2, most pronounced in the group with BMP-2 and diopside on the model of critical size defects in the cranium in mice. Thus, the use of 3D scaffolds based on recombinant spidroin with BMP2-loaded diopside particles in hyaluronic acid can lead to effective induction of osteogenesis. The spidroin scaffolds themselves represent a sufficiently effective carrier for BMP-2.

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来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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