Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model

{"title":"Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model","authors":"A. S. Karyagina,&nbsp;V. G. Bogush,&nbsp;P. A. Orlova,&nbsp;L. I. Davydova,&nbsp;A. V. Zhulina,&nbsp;T. M. Grunina,&nbsp;N. V. Strukova,&nbsp;M. S. Generalova,&nbsp;M. S. Krivozubov,&nbsp;S. E. Cheperegin,&nbsp;A. A. Ramonova,&nbsp;A. V. Gromov","doi":"10.1134/S000368382470008X","DOIUrl":null,"url":null,"abstract":"<p><b>Abstract</b>—Calcium–magnesium silicate ceramic diopside is an effective carrier of BMP-2 and, in combination with a bovine demineralized bone matrix as a scaffold, can induce effective reparative osteogenesis on the model of craniotomy in mice. It was of interest to investigate the efficacy of combined usage of BMP-2-loaded diopside with recombinant spidroins for the induction of osteogenesis. For this purpose, porous implants were prepared based on recombinant spidroin rS1/9, into which BMP-2 was introduced either as BMP-2 loaded diopside particles suspended in hyaluronic acid or by adsorption. Neo-osteogenesis with bone marrow formation occurred in both groups with BMP-2, most pronounced in the group with BMP-2 and diopside on the model of critical size defects in the cranium in mice. Thus, the use of 3D scaffolds based on recombinant spidroin with BMP2-loaded diopside particles in hyaluronic acid can lead to effective induction of osteogenesis. The spidroin scaffolds themselves represent a sufficiently effective carrier for BMP-2.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 7","pages":"1493 - 1503"},"PeriodicalIF":1.0000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S000368382470008X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}