E. V. Patrusheva, L. N. Borshchevskaya, A. A. Kanikovskaya, S. P. Sineoky
{"title":"Molecular Serotyping of Bacillus turingiensis","authors":"E. V. Patrusheva, L. N. Borshchevskaya, A. A. Kanikovskaya, S. P. Sineoky","doi":"10.1134/S0003683824700157","DOIUrl":null,"url":null,"abstract":"<p> A method for molecular serotyping of <i>Bacillus turingiensis</i> strains has been developed. It is based on comparison of nucleotide sequences of conserved regions of the <i>flaA</i> gene in five <i>B.</i> <i>turingiensis</i> serovars: <i>sotto</i>, <i>kurstaki</i>, <i>israilensis</i>, <i>berliner</i>, and <i>morrisoni.</i> For amplification of the target DNA fragment, a set of primers universal for all studied serovars of <i>B.</i> <i>turingiensis</i> was designed. To assign a strain to a particular serovar, a new algorithm based on the degree of homology of the conserved <i>flaA</i> region was developed. Validation of the method was carried out on 11 strains of <i>B.</i> <i>turingiensis</i> from the collection of the National Bioresource Center All-Russia National Collection of Industrial Microorganisms (VKPM). The results obtained by molecular serotyping fully coincided with the data of serological methods.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1551 - 1557"},"PeriodicalIF":1.0000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824700157","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A method for molecular serotyping of Bacillus turingiensis strains has been developed. It is based on comparison of nucleotide sequences of conserved regions of the flaA gene in five B.turingiensis serovars: sotto, kurstaki, israilensis, berliner, and morrisoni. For amplification of the target DNA fragment, a set of primers universal for all studied serovars of B.turingiensis was designed. To assign a strain to a particular serovar, a new algorithm based on the degree of homology of the conserved flaA region was developed. Validation of the method was carried out on 11 strains of B.turingiensis from the collection of the National Bioresource Center All-Russia National Collection of Industrial Microorganisms (VKPM). The results obtained by molecular serotyping fully coincided with the data of serological methods.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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