{"title":"Use of Methyl Green in Flow Cytometry to Control the Cell Viability of C. glutamicum","authors":"A. S. Derunets, D. D. Derbikov, A. S. Yanenko","doi":"10.1134/S0003683824700169","DOIUrl":null,"url":null,"abstract":"<p>The use of methyl green dye in flow cytometry to monitor the viability of <i>Corynebacterium glutamicum</i> cells was studied. The use of methyl green has an important advantage—its spectra do not overlap with the fluorescence spectra of other fluorophores. Our study demonstrated that the fluorescence spectra of live cells of <i>Corynebacterium</i> do not overlap with the spectra of dead cells stained with methyl green, which is a significant benefit of such use in flow cytometry to monitor the viability of <i>Corynebacterium glutamicum</i> cells. It has been established that the optimal concentration of methyl green for staining <i>C. glutamicum</i> cells is in the range of 5–15 μg/mL. Under these conditions, methyl green is nontoxic to corynebacterial cells, which is confirmed by the coincidence of the titer (number) of living cells determined using flow cytometry or a standard microbiological method based on growth on the nutrient medium.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 8","pages":"1558 - 1564"},"PeriodicalIF":1.0000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824700169","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The use of methyl green dye in flow cytometry to monitor the viability of Corynebacterium glutamicum cells was studied. The use of methyl green has an important advantage—its spectra do not overlap with the fluorescence spectra of other fluorophores. Our study demonstrated that the fluorescence spectra of live cells of Corynebacterium do not overlap with the spectra of dead cells stained with methyl green, which is a significant benefit of such use in flow cytometry to monitor the viability of Corynebacterium glutamicum cells. It has been established that the optimal concentration of methyl green for staining C. glutamicum cells is in the range of 5–15 μg/mL. Under these conditions, methyl green is nontoxic to corynebacterial cells, which is confirmed by the coincidence of the titer (number) of living cells determined using flow cytometry or a standard microbiological method based on growth on the nutrient medium.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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