Analytical biochemistry最新文献

Glycosyltransferase enzymatic assays: Overview and comparative analysis

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-04 DOI: 10.1016/j.ab.2025.115826

Ghazal Khaled , Thierry Benvegnu , Khadija Amin , Sylvain Tranchimand , Hala Chamieh

{"title":"Glycosyltransferase enzymatic assays: Overview and comparative analysis","authors":"Ghazal Khaled , Thierry Benvegnu , Khadija Amin , Sylvain Tranchimand , Hala Chamieh","doi":"10.1016/j.ab.2025.115826","DOIUrl":"10.1016/j.ab.2025.115826","url":null,"abstract":"<div><div>Glycosyltransferases (GTs) are enzymes that catalyze the transfer of an activated sugar donor to a variety of acceptors including proteins, lipids, carbohydrates, and other small molecules. GTs participate in numerous cellular and physiological processes in both prokaryotic and eukaryotic cells. Those include prokaryotic cell wall biogenesis, eukaryotic post-translational protein modifications, extracellular matrix synthesis, cell signaling, biofilm formation and many others. As such, GTs are exploited as molecular therapeutical targets but also as synthetic tools for the development of polysaccharides and glycoconjugates. <em>In vitro</em> study of GTs activities is now essential to characterize the growing number of predicted GTs, available from sequenced genomes, in order to determine their specificities, their modes of action and their roles <em>in vivo</em>. However, characterization of glycosyltransferases <em>in vitro,</em> both on cellular extracts and on purified enzymes, faces significant challenges. Many methods are currently employed <em>i. e.</em> radiochemical techniques, spectrometric measurements, generally after coupling with∗ other reactions, and even more sophisticated strategies involving product separations by chromatography or/and electrophoresis, followed by detailed structural analysis by NMR or mass spectrometry. Here we overview the common methods deployed for the characterization of GTs. We highlight the challenges arising from these enzymes. The advantages and limitations of each of the presented techniques are also discussed.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115826"},"PeriodicalIF":2.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical aptasensor for determination of testosterone using an aptamer-nanogold-metal-organic framework-ionic liquid modified carbon paste electrode.

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-03 DOI: 10.1016/j.ab.2025.115827

Samira Nekooie, Mehrorang Ghaedi, Mohammad Reza Baezat, Javad Tashkhourian, Mika Sillanpää

{"title":"Electrochemical aptasensor for determination of testosterone using an aptamer-nanogold-metal-organic framework-ionic liquid modified carbon paste electrode.","authors":"Samira Nekooie, Mehrorang Ghaedi, Mohammad Reza Baezat, Javad Tashkhourian, Mika Sillanpää","doi":"10.1016/j.ab.2025.115827","DOIUrl":"https://doi.org/10.1016/j.ab.2025.115827","url":null,"abstract":"<p><p>The present study is based on design and construction of a selective label-free electrochemical aptasensor for the testosterone (TST) detection in real samples. A carbon paste electrode modified with gold nanoparticles, metal-organic framework-ionic liquid (AuNPs/Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Cu-ILCPE) was used to covalently immobilize the TST aptamer (TST-apt). The AuNPs/Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Cu-ILCPE represents distinguished ability for enhancement of electrochemical peak current, which may emerge from the aptamer on the electrode surface. Initially, the dependency of signals to variables like the aptamer concentration, aptamer immobilization time, AuNPs electrodeposion time, and aptamer reaction time was investigated and optimized. Under the optimized conditions, the proposed aptasensor has two linear ranges over 1.0-50.0 nM and 50.1-1000.0 nM with limit of detection of 0.31 nM, and the limit of quantification of 0.99 nM. The aptasensor was also applicable to detecting TST in urine samples with satisfactory recoveries of 97.2-103.8%.</p>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":" ","pages":"115827"},"PeriodicalIF":2.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing protein heterogeneity analysis: Nano-flow pressure mobilization for precise icIEF fractionation and online MS detection

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-02 DOI: 10.1016/j.ab.2025.115825

Teresa Kwok, She Lin Chan, Niusheng Xu, Tiemin Huang, Tao Bo

{"title":"Advancing protein heterogeneity analysis: Nano-flow pressure mobilization for precise icIEF fractionation and online MS detection","authors":"Teresa Kwok, She Lin Chan, Niusheng Xu, Tiemin Huang, Tao Bo","doi":"10.1016/j.ab.2025.115825","DOIUrl":"10.1016/j.ab.2025.115825","url":null,"abstract":"<div><div>This study addresses the challenges of high-resolution protein charge variant fractionation and efficient online mass spectrometry (MS) detection in imaged capillary isoelectric focusing (icIEF)-based workflows. icIEF often faces limitations in efficiency, peak integrity, and detection sensitivity due to diffusion and uncontrolled mobilization. To overcome these, we developed a novel icIEF fractionation framework that integrates nano-flow pressure mobilization with the capillary diameter transformation technique (CDTT). Using a model system with a 320 μm ID separation channel and a 50 μm ID transfer capillary, we investigated the electrophoretic and nano-flow transport mechanisms influencing fractionation efficiency. The impact of these innovations on peak area, height, and width for charge proteoforms was assessed, showing improvements in precision. These insights were applied to a 200 μm ID separation channel system, resulting in enhanced separation efficiency and icIEF-MS sensitivity. This study offers a scalable, high-precision solution for charge heterogeneity analysis in biopharmaceutical development and regulatory applications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115825"},"PeriodicalIF":2.6,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smartphone-based colorimetric correction analysis system for long-term urine monitoring of chronic kidney disease.

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-28 DOI: 10.1016/j.ab.2025.115824

Hong Xu, Yiran Hua, Haiqin Li, Jianhua Wang, Gaohong Liu, Xiaochun Li

{"title":"Smartphone-based colorimetric correction analysis system for long-term urine monitoring of chronic kidney disease.","authors":"Hong Xu, Yiran Hua, Haiqin Li, Jianhua Wang, Gaohong Liu, Xiaochun Li","doi":"10.1016/j.ab.2025.115824","DOIUrl":"https://doi.org/10.1016/j.ab.2025.115824","url":null,"abstract":"<p><p>The chronic kidney disease (CKD) poses a serious threat to human health. Long-term monitoring of urine is important in the management of CKD. Currently, the accuracy and stability of smartphone-based colorimetric analysis of urine indicators are limited due to the impact of different image-taking conditions on captured digital images of urine test strips. Herein, an attachment-free colorimetric correction analysis system (CCAS for short), consisting of a self-designed urine test strip array and an Android application integrated with an image calibration algorithm, were proposed for quantitative analysis of nine urine indicators. With this system the impact of image-taking conditions on captured digital image were largely corrected, and thus the accuracy and stability for digital image colorimetric analysis of urine test strip were improved. The limits of detection of creatinine, nitrite, urinary calcium, microalbumin, bilirubin, protein, pH, haemoglobin, and glucose were 1.607 mmol/L, 1.232 μmol/L, 0.297 mmol/L, 11.116 mg/L, 1.155 μmol/L, 0.042 g/L, 0.044, 0.058 mg/L, and 0.122 mmol/L, respectively. The accuracy of CCAS was validated by analyzing artificial urine samples and 143 clinical urine samples. As an accurate, low-cost and reliable system, CCAS addresses specific needs for patients to monitor their urine whenever and wherever possible with only their own smartphone.</p>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":" ","pages":"115824"},"PeriodicalIF":2.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic platforms for snakebite: current approaches and challenges in medically important species. 蛇咬伤诊断平台：重要医学物种的现有方法和挑战。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-26 DOI: 10.1016/j.ab.2025.115823

Nairo Brilhante-da-Silva, Sibele Andrade Roberto, Nidiane Dantas Reis Prado, Anna Carolina Machado Marinho, Carla Freire Celedonio Fernandes, Soraya Dos Santos Pereira

{"title":"Diagnostic platforms for snakebite: current approaches and challenges in medically important species.","authors":"Nairo Brilhante-da-Silva, Sibele Andrade Roberto, Nidiane Dantas Reis Prado, Anna Carolina Machado Marinho, Carla Freire Celedonio Fernandes, Soraya Dos Santos Pereira","doi":"10.1016/j.ab.2025.115823","DOIUrl":"https://doi.org/10.1016/j.ab.2025.115823","url":null,"abstract":"<p><p>Diagnosing snakebite envenoming is a critical aspect of managing this life-threatening condition. Achieving the impacts and clinical applications of the diagnosis of snakebites is important to help clarify the challenges faced in identifying venomous snakebites and implementing effective treatment strategies. The complexity of the envenomation requires a comprehensive diagnostic approach, considering both clinical symptoms and laboratory findings. Despite advances in diagnostic technologies, the lack of standardized tools represents a significant obstacle to obtaining accurate and timely assessments. This paper analyzes the state-of-the-art snakebite diagnosis, emphasizing the need for methodologies for differential diagnosis of snakebites. Furthermore, it investigates the far-reaching consequences of late or incorrect diagnoses, emphasizing their role in the potential development of serious morbidity and even mortality. The discussion extends to the global health burden caused by snakebite envenoming, emphasizing the importance of personalized diagnostic methods in regions with diverse snake species. Furthermore, the review explores recent advances in the global scale diagnosis of snakebite venom. The clinical applications of these innovations are explored, highlighting their potential to revolutionize snakebite management in resource-limited settings. By addressing the multifaceted challenges in snakebite diagnosis, this summary contributes to ongoing efforts to mitigate the global impact of snakebite envenoming by highlighting the critical need for collaborative research, standardization, and accessibility of diagnostic tools.</p>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":" ","pages":"115823"},"PeriodicalIF":2.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous determination of 25(OH)D2, 25(OH)D3 and 1α,25(OH)2D3 in human serum by derivatization-liquid chromatography-tandem mass spectrometry

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-24 DOI: 10.1016/j.ab.2025.115821

Zi-qing Wang , Li-ping Hao , Zi-xuan Meng , Hao-ran Zhang , Wei-jun Kang , Lian-feng Ai

{"title":"Simultaneous determination of 25(OH)D2, 25(OH)D3 and 1α,25(OH)2D3 in human serum by derivatization-liquid chromatography-tandem mass spectrometry","authors":"Zi-qing Wang , Li-ping Hao , Zi-xuan Meng , Hao-ran Zhang , Wei-jun Kang , Lian-feng Ai","doi":"10.1016/j.ab.2025.115821","DOIUrl":"10.1016/j.ab.2025.115821","url":null,"abstract":"<div><div>An LC-MS/MS method was developed and validated for simultaneously quantifying 25-hydroxyvitamin D2, D3, and 1α,25-dihydroxyvitamin D3 in human serum. Protein in 200 μL serum was precipitated with acetonitrile. After centrifugation, the metabolites were derivatized using (diacetoxyiodo)benzene (PIA) and 4-(4-dimethylaminophenyl)-1,2,4-triazolidine-3,5-dione (DMAT) and then quantified by the LC-MS/MS system. The limits of detection (LODs) for the three substances were 10, 10, and 5 pg/mL, and the limits of quantification (LOQs) were 20, 20, and 10 pg/mL. The standard curves for these compounds showed linear regression coefficients (R2)>0.998 over specific concentration ranges. Recoveries were 94.36 %–102.34 % for 25(OH)D2, 92.43 %–103.41 % for 25(OH)D3, and 88.98 %–94.36 % for 1α,25(OH)2D3. The mean serum levels in 109 subjects (consisting of 61 healthy adult males and 59 healthy adult females) were 2.0 ± 1.5 ng/ml (25(OH)D2), 16.4 ± 6.1 ng/ml (25(OH)D3) and 36.6 ± 15.1 pg/ml (1α, 25(OH)2D3).Derivatization of vitamin D metabolites using PIA and DMAT is useful for rapidly determining the serum 25(OH) D2, 25(OH) D3 and 1α,25(OH)2D3 concentrations simultaneously in human serum.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115821"},"PeriodicalIF":2.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total histamine degrading capacity: Correlation to stool histamine and its usefulness in supporting the diagnosis of histamine intolerance

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-22 DOI: 10.1016/j.ab.2025.115819

Gabriele Frost , Christian Lutz , Andreas Schwiertz , Hermann Huber , Albert Missbichler

{"title":"Total histamine degrading capacity: Correlation to stool histamine and its usefulness in supporting the diagnosis of histamine intolerance","authors":"Gabriele Frost , Christian Lutz , Andreas Schwiertz , Hermann Huber , Albert Missbichler","doi":"10.1016/j.ab.2025.115819","DOIUrl":"10.1016/j.ab.2025.115819","url":null,"abstract":"<div><div>The relatively new test (European Patent granted 2022) for determining the total histamine degradation capacity [Totale Histamin Abbau Kapazität/Total Histamine Degrading Capacity] THAK®/THDC of a serum sample has been compared in various settings with the individual clinical history and the levels of histamine in stool. The measurement of THAK can be carried out regardless of the current status of the patient (independent of any acute symptoms), therefore THAK test is a useful and very reliable instrument for supporting the diagnosing histamine intolerance. As soon as the THAK falls below a threshold value the patient is most likely to suffer from HIT, even if the intensity of the symptoms is low. Low THAK values correspond with a significant increase of histamine in stool. Results were evaluated according to both age and gender, no difference between the sexes were found. However, there is a noticeable dependency on age, although this is not diagnostically relevant.</div><div>THAK® is a registered trademark of Frost Diagnostika.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115819"},"PeriodicalIF":2.6,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of a multiplexed assay for six urinary proteins based on liquid microarray analysis technology

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-21 DOI: 10.1016/j.ab.2025.115820

Peng Zhang , Yi Liu , Xinyun Zhou , Yinyu Chen , Liudi Pan , Feiyi Xu , Xiaolong Zhang , Lisong Shen , Hui Chen

{"title":"Evaluation of a multiplexed assay for six urinary proteins based on liquid microarray analysis technology","authors":"Peng Zhang , Yi Liu , Xinyun Zhou , Yinyu Chen , Liudi Pan , Feiyi Xu , Xiaolong Zhang , Lisong Shen , Hui Chen","doi":"10.1016/j.ab.2025.115820","DOIUrl":"10.1016/j.ab.2025.115820","url":null,"abstract":"<div><div>Proteinuria serves as a critical indicator in the progression of chronic kidney disease. The quantification of urinary protein is instrumental in diagnosing kidney disease and monitoring therapeutic efficacy. Nephelometry and turbidimetric immunoassay are predominantly employed for the quantitative detection of various urinary proteins. We developed a quantitative detection method for six proteins in urine utilizing the flow fluorescence luminescence method on the Luminex®200™ detection platform. This study aims to evaluate the clinical performance and accuracy of the method. The sensitivity, precision, recovery, linearity, and interference metrics for the method meet the needs of clinical applications. The correlation coefficient (r) between the results of the method and nephelometry immunoassay on SIEMENS BNII platform ranged from 0.9375 to 0.9847, indicating a strong linear relationship. The results of the Bland-Altman analysis indicated that the systematic bias between the two methods was minimal. Additionally, Passing-Bablok regression analysis, both including and excluding outliers, was conducted on the clinical trial data. The findings demonstrated consistency between the two methods, with the expected confidence interval of bias remaining within the allowable error limits, indicating no significant discrepancy. This urine six protein quantification method demonstrates satisfactory performance, meeting the standards necessary for medical diagnosis and clinical application.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115820"},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A drop-like optical fiber salivary pH sensor: A rapid and real-time detection of periodontal disease

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-20 DOI: 10.1016/j.ab.2025.115818

Huda Abdulsahib Sabbar, Sarah Kadhim Al-Hayali, Abdulhadi Al-Janabi

{"title":"A drop-like optical fiber salivary pH sensor: A rapid and real-time detection of periodontal disease","authors":"Huda Abdulsahib Sabbar, Sarah Kadhim Al-Hayali, Abdulhadi Al-Janabi","doi":"10.1016/j.ab.2025.115818","DOIUrl":"10.1016/j.ab.2025.115818","url":null,"abstract":"<div><div>A real-time and rapid detection of <strong>s</strong>alivary pH for periodontal diagnosis is an emerging point-of-care (POC) technology trend. The precise and non-invasive sensitive POC devices, which could be used as chair-side tools to improve clinical dental inspections, are essential for an excellent periodontal diagnosis. In this paper, we report on developing a POC technology based on optical fiber sensors as a promising marker, to the best of our knowledge, to detect the role of salivary pH in periodontitis diagnosis and support dentists’ inspection. The optical fiber sensor based on a drop-like structure was fabricated by bending single-mode fiber (SMF) into a section of a thin capillary tube. In this work, firstly, the performance of the proposed sensor towards different pH levels ranging from 1 to 7 was experimentally investigated. Experimentations showed that the pH sensor responded to various pH levels with a calibration curve sensitivity of −2.075 nm/pH and a high linearity of 0.985. Then, 66 patients were enrolled in this study and divided into two groups according to clinical outcomes: Group A with clinically healthy periodontium and Group B with chronic periodontitis. The real saliva from each patient was collected, and the salivary pH was evaluated immediately using the aforementioned proposed sensor. The experimental results show that Group A and Group B salivary pH mapping ranged from 6.5 to 7.3 and 5.5 to 6.4, respectively. The proposed sensor offers substantial practical benefits, providing a rapid response time of less than 2 s, instantaneous, noninvasive, and easily monitored tool for the early detection and diagnosis of periodontal health status.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115818"},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a graphene quantum dot-based sensor for abacavir quantification via fluorescence quenching

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-02-14 DOI: 10.1016/j.ab.2025.115817

Ali Alqahtani , Taha Alqahtani , Adel Al Fatease , Ahmed A. Almrasy

{"title":"Development and validation of a graphene quantum dot-based sensor for abacavir quantification via fluorescence quenching","authors":"Ali Alqahtani , Taha Alqahtani , Adel Al Fatease , Ahmed A. Almrasy","doi":"10.1016/j.ab.2025.115817","DOIUrl":"10.1016/j.ab.2025.115817","url":null,"abstract":"<div><div>Human immunodeficiency virus (HIV) is a global health concern, and the development of effective methods for the detection and quantification of antiretroviral drugs is crucial for monitoring therapeutic efficacy and patient compliance. Herein, we present a novel approach utilizing graphene quantum dots (GQDs) for the determination of abacavir, a widely used nucleoside reverse transcriptase inhibitor in the treatment of HIV. The sensing mechanism was investigated through Stern-Volmer analysis, thermodynamics studies, and density functional theory calculations, which revealed the strong binding interactions between GQDs and abacavir <em>via</em> a static quenching process. Factors affecting the analytical performance, such as pH, GQDs concentration, and incubation time, were systematically optimized to achieve a linear detection range of 100–1000 ng/mL with a low detection limit of 17.49 ng/mL. The method was validated in accordance with ICH guidelines demonstrating excellent linearity, accuracy, precision, robustness and selectivity making it suitable for the quantification of abacavir in pharmaceutical formulations and biological samples. The method also demonstrated higher sensitivity and more environmentally friendly characteristics when compared to previously reported chromatographic techniques, showcasing the potential of GQDs as a superior alternative for the traditional detection approaches of pharmaceutical compounds.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115817"},"PeriodicalIF":2.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0