Analytical biochemistry最新文献_第2页

Biosynthesised reduced graphene oxide/CuO based nanocomposite using ‘Cordia dichotoma’ leaf extract for ‘nitrobenzene’ determination 生物合成还原氧化石墨烯/CuO基纳米复合材料，使用“Cordia dichotoma”叶提取物测定“硝基苯”

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-10 DOI: 10.1016/j.ab.2025.115972

Devkumari Patel , Robind Kumar , Sanju Yadav , Ankita Rai , Vijai K. Rai , Manorama Singh

{"title":"Biosynthesised reduced graphene oxide/CuO based nanocomposite using ‘Cordia dichotoma’ leaf extract for ‘nitrobenzene’ determination","authors":"Devkumari Patel , Robind Kumar , Sanju Yadav , Ankita Rai , Vijai K. Rai , Manorama Singh","doi":"10.1016/j.ab.2025.115972","DOIUrl":"10.1016/j.ab.2025.115972","url":null,"abstract":"<div><div>Herein, plant-assisted synthesis of copper oxide nanoparticles (CuO NPs)<sub>CD</sub> and reduced graphene oxide (CDrGO) was performed individually using the leaf extract of ‘<em>Cordia dichotoma’</em> plant and they were associated with electrochemically active conducting clay ‘Sodium-Montmorillonite (Na-MT)’ leading to the synthesis of a new nanocomposite named ‘(CuO NPs)<sub>CD</sub>@CDrGO-Na-MT’. The collaboration of CDrGO paired (CuO NPs)<sub>CD</sub> and Na-MT enhanced the electrochemical reduction of noxious ‘Nitrobenzene (NB)’ at a lower potential. The prepared ternary nanocomposite was confirmed with characterisation techniques, Transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Electrochemical properties and electrochemical reduction of ‘Nitrobenzene’ were inspected with cyclic voltammetry and differential pulse voltammetry techniques, respectively. The electro-reduction of NB was recorded in two wide linear ranges of 0.016–360 μM and 360–6980 μM with a detection limit of 15 nM and negligible effect of interferences.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115972"},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping transposon insertion sites within bacterial genomes by direct Sanger sequencing 通过直接Sanger测序在细菌基因组中定位转座子插入位点。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-10 DOI: 10.1016/j.ab.2025.115971

Scott W. Herke , Linda M. Heffernan , William N. Beavers , Basel H. Abuaita

{"title":"Mapping transposon insertion sites within bacterial genomes by direct Sanger sequencing","authors":"Scott W. Herke , Linda M. Heffernan , William N. Beavers , Basel H. Abuaita","doi":"10.1016/j.ab.2025.115971","DOIUrl":"10.1016/j.ab.2025.115971","url":null,"abstract":"<div><div>Microbial biomedical research frequently involves mutagenesis by insertion of transposons into the genome. Currently, transposon insertion locations are typically elucidated by Next Generation Sequencing or by Sanger sequencing of PCR products. Here, transposons were located by direct Sanger sequencing of bacterial genomic DNA from both <em>Salmonella enterica</em> (Gram-negative) and <em>Staphylococcus aureus</em> (Gram-positive) cultures. DNA was prepared by shearing to a modal size of ∼2 kb followed by purification by paramagnetic beads. Sequencing reactions involved relatively minor modifications of standard protocols (e.g., extra sequencing polymerase, 75–100 PCR cycles); completed reactions were cleaned by ethanol-EDTA precipitation. Reads were generated on the ABI 3130xl Genetic Analyzer using 50-cm capillary arrays and a run protocol modified for extra sample injection time. Good quality reads of ∼500–800 nt were routinely generated; BLAST results returned nearly 100% matches to genomes in the NCBI database. As implemented, the optimized protocol (post-DNA extraction) could be performed within an 8-h workday (with sequencing results the following day) for ∼$10 (USD) per sequencing reaction. Although this method was developed to locate transposons inserted into bacterial genomes, it seems likely that it can be extended to generate sequence data from even native single-copy genes from small genomes (e.g., <5 Mb).</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115971"},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Easy reference-guided assembly of nanopore whole plasmid sequencing datasets 易于参考引导组装纳米孔全质粒测序数据集。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-05 DOI: 10.1016/j.ab.2025.115970

Vinita Sharma , Naiyu Jiang , Yukihiro Nagashima , Hisashi Koiwa

引用次数: 0

PreRBP: Interpretable deep learning for RNA-protein binding site prediction with attention mechanism PreRBP：基于注意机制的rna -蛋白结合位点预测的可解释深度学习。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-04 DOI: 10.1016/j.ab.2025.115968

Huixian Chen , Yun Zuo , Xiangrong Liu , Xiangxiang Zeng , Zhaohong Deng , Jiasong Wu

{"title":"PreRBP: Interpretable deep learning for RNA-protein binding site prediction with attention mechanism","authors":"Huixian Chen , Yun Zuo , Xiangrong Liu , Xiangxiang Zeng , Zhaohong Deng , Jiasong Wu","doi":"10.1016/j.ab.2025.115968","DOIUrl":"10.1016/j.ab.2025.115968","url":null,"abstract":"<div><div>In the complex process of gene expression and regulation, RNA-binding proteins occupy a pivotal position for RNA. Accurate prediction of RNA-protein binding sites can help researchers better understand RNA-binding proteins and their related mechanisms. And prediction techniques based on machine learning algorithms are both cost-effective and efficient in identifying these binding sites. However, there are some shortcomings in the currently available machine learning methods, such as the input features of the model only consider RNA sequence features, and most of the datasets suffer from class imbalance. To address these issues, this study first uses the publicly available 27 RNA-protein binding site datasets to construct a benchmark dataset. Then, we use RNAshapes and EDeN to obtain the secondary structure of RNA. Higher-order encoding method is used to extract the key information hidden in the RNA sequences and structures. In order to solve the class imbalance problem existing in the dataset, this study utilizes four undersampling algorithms, namely, random undersampling, NearMiss, ENN, and one-sided selection, to remove redundant samples in the negative samples, and lastly, based on Convolutional Neural Network, Bidirectional Long and Short Term Memory Network, this study constructs model PreRBP to predict RNA-protein binding sites.</div><div>The experimental results show that the model used in this study has an average AUC of 0.88, which is higher than other existing RNA-protein binding site prediction methods. Also, for the convenience of prediction, an online predictor is developed in this study. The predictor and experimental codes are available at <span><span>https://github.com/B12-Comet/RBPPrediction</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"707 ","pages":"Article 115968"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of endoplasmic reticulum stress-related gene markers in amyotrophic lateral sclerosis: a comprehensive analysis of bioinformatics and machine learning 肌萎缩侧索硬化症内质网应激相关基因标记的探索：生物信息学和机器学习的综合分析

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-04 DOI: 10.1016/j.ab.2025.115969

Jing Wang , Xinmin Li , Fangjie Yang , Pengxue Guo , Chunlin Ren , Zhengfei Duan , Mengyao Bi , Yuting Kong , Yasu Zhang , Jianwei Lu

{"title":"Exploration of endoplasmic reticulum stress-related gene markers in amyotrophic lateral sclerosis: a comprehensive analysis of bioinformatics and machine learning","authors":"Jing Wang , Xinmin Li , Fangjie Yang , Pengxue Guo , Chunlin Ren , Zhengfei Duan , Mengyao Bi , Yuting Kong , Yasu Zhang , Jianwei Lu","doi":"10.1016/j.ab.2025.115969","DOIUrl":"10.1016/j.ab.2025.115969","url":null,"abstract":"<div><div>This study aimed to investigate potential biomarkers related to Endoplasmic reticulum (ER) stress in Amyotrophic lateral sclerosis (ALS) through a comprehensive bioinformatic approach. The gene expression profiles of ALS patients and healthy controls were downloaded from the Gene Expression Omnibus (GEO) database. ER stress-related genes were collected from the MSigDB databases and document literature. The “limma” R package was employed to detect the differentially expressed ER stress-related genes (DE-ERSGs). Three methods of machine learning were applied to select the hub DE-ERSGs. ROC curves were conducted to evaluate model performance. An external dataset was chosen to evaluate the diagnostic capability of hub genes. The CIBERSORT algorithm was used to evaluate the immune cell infiltration characteristics. Additionally, we constructed a systematic ceRNA regulatory network using Cytoscape software and predicted the possible drug candidates using the Enrichr platform. Molecular docking analysis was used to further validate the binding ability of the candidate drug molecules to the hub genes. Six hub DE-ERSGs (ABCA1, CKAP4, TOR1AIP1, MMP9, EDC4, and ALPP) were identified, and the related models performed well. These hub genes were concentrated in multiple pathways and related to various immune cells. Drugs such as nitroglycerin, diazepam, FENRETINIDE, and edaravone exhibited good binding affinity to the hub genes, indicating that they may be promising drugs for the management of ALS. This study revealed the essential role of ER stress in the pathogenesis of ALS from an integrative perspective, providing guidance for the development of new therapeutic targets and diagnostic strategies.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"707 ","pages":"Article 115969"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mismatch-sensitive DNA hybridization controlled by inchworm-type peptide nucleic acid–PEG conjugates 尺蠖型肽核酸- peg偶联物控制错配敏感DNA杂交

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-04 DOI: 10.1016/j.ab.2025.115967

Toshihiko Sakurai , Yusuke Hamashita , Takahiro Shibata

{"title":"Mismatch-sensitive DNA hybridization controlled by inchworm-type peptide nucleic acid–PEG conjugates","authors":"Toshihiko Sakurai , Yusuke Hamashita , Takahiro Shibata","doi":"10.1016/j.ab.2025.115967","DOIUrl":"10.1016/j.ab.2025.115967","url":null,"abstract":"<div><div>The duplex-forming behavior of an inchworm-type PNA–PEG conjugate (i-PPc), engineered for the selective recognition of point mutations in DNA, was assessed through thermodynamic analysis employing UV melting curves and circular dichroism spectroscopy. The i-PPc demonstrated the ability to form stable duplexes exclusively with fully complementary DNA sequences, while no hybridization with single-base mismatched sequences. This binary on/off hybridization behavior was maintained even under physiologically relevant conditions (37 °C), thereby illustrating the exceptional point mutation discrimination capability of i-PPc. The behavior observed can be ascribed to the distinctive structure of i-PPc, wherein two PNA segments, possessing intrinsically different duplex-forming stabilities—high and low—are covalently linked via a flexible PEG linker. The high-stability PNA segment functions as the primary recognition domain for point mutations, thereby defining the sequence specificity of duplex formation. Conversely, the low-stability segment contributes cooperatively to the overall duplex stabilization only when the high-stability segment successfully hybridizes with the target DNA. This cooperative mechanism underlies the sequence-selective duplex formation of i-PPc, highlighting its potential as a highly specific probe for DNA mutation diagnostics. These findings indicate that i-PPc represents a promising platform for point mutation detection and nucleic acid-based molecular diagnostics grounded in DNA hybridization under physiological conditions.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"707 ","pages":"Article 115967"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous assay for the dNTP triphosphohydrolase of activated SAMHD1 活化SAMHD1的dNTP三磷酸水解酶连续测定。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-09-01 DOI: 10.1016/j.ab.2025.115966

Roozbeh Eskandari, Daniel P. Groom, Ryo Tamura, Vern L. Schramm

{"title":"Continuous assay for the dNTP triphosphohydrolase of activated SAMHD1","authors":"Roozbeh Eskandari, Daniel P. Groom, Ryo Tamura, Vern L. Schramm","doi":"10.1016/j.ab.2025.115966","DOIUrl":"10.1016/j.ab.2025.115966","url":null,"abstract":"<div><div>Sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) is the only member of the triphosphoric monoester hydrolase family in humans (dNTP + H<sub>2</sub>O → dN + PPPi). The dNTPase activity of SAMHD1 inhibits DNA synthesis, resulting in cell-cycle arrest and restricting viral replication. The complex allosteric regulation mechanism of SAMHD1 and a reaction that lacks a direct spectroscopic signal make its kinetic analysis and inhibitor discovery challenging. We describe a continuous assay for monitoring SAMHD1 phosphatase activity in its activated physiological state. The assay uses a sequential assembly to generate the active tetrameric form of the enzyme. Two phosphatases convert inorganic triphosphate (PPPi) to inorganic phosphate (Pi). The released Pi reacts with the 7-methyl-6-thioguanosine and purine nucleoside phosphorylase to provide a sensitive continuous spectrophotometric assay. The assay is suitable for 96-microwell plate formats to provide a continuous measurement of SAMHD1 activity. The assay is benchmarked with inhibitors of SAMHD1. With a Z-prime value > 0.90, the assay can be used for high-throughput screening of inhibitors for SAMHD1 and characterizing the allosteric or catalytic activity of the new inhibitors.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115966"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron nitride quantum dot-based fluorescent sensor for carbamazepine determination in exhaled breath condensate 氮化硼量子点荧光传感器测定呼出液中卡马西平

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-08-26 DOI: 10.1016/j.ab.2025.115964

Saba Ershadi , Maryam Khoubnasabjafari , Vahid Jouyban-Gharamaleki , Elaheh Rahimpour , Abolghasem Jouyban

{"title":"Boron nitride quantum dot-based fluorescent sensor for carbamazepine determination in exhaled breath condensate","authors":"Saba Ershadi , Maryam Khoubnasabjafari , Vahid Jouyban-Gharamaleki , Elaheh Rahimpour , Abolghasem Jouyban","doi":"10.1016/j.ab.2025.115964","DOIUrl":"10.1016/j.ab.2025.115964","url":null,"abstract":"<div><div>Carbamazepine is a widely prescribed antiepileptic drug with a narrow therapeutic index, necessitating precise monitoring to avoid toxicity and ensure therapeutic efficacy. This study presents a fluorescence-based nanosensor using boron nitride quantum dots (BNQDs) for the rapid and sensitive detection of carbamazepine in exhaled breath condensate (EBC). BNQDs were prepared via a simple hydrothermal technique and characterized using transmission electron microscopy, dynamic light scattering, energy-dispersive X-ray, and attenuated total reflectance-Fourier transform infrared techniques. The sensor exhibited a concentration-dependent quenching of BNQD fluorescence upon carbamazepine addition, with a linear response range of 0.2–2.4 μg mL<sup>−1</sup> and a low detection limit of 0.05 μg mL<sup>−1</sup>. Stern–Volmer analysis confirmed a dynamic quenching mechanism. The nanosensor also demonstrated high selectivity against common co-administered drugs and was successfully applied to real EBC samples collected from patients receiving carbamazepine therapy. This BNQD-based sensing platform offered a rapid, cost-effective, and user-friendly approach for the non-invasive therapeutic drug monitoring of carbamazepine.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"707 ","pages":"Article 115964"},"PeriodicalIF":2.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ITGB3 as a promising non-invasive biomarker for type 2 diabetes and diabetic nephropathy ITGB3有望成为2型糖尿病和糖尿病肾病的无创生物标志物

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-08-25 DOI: 10.1016/j.ab.2025.115965

Seyed Amirhossein Hosseini , Parisa Ajorlou , Hasti Haddadian , Shahla Sohrabipour

{"title":"ITGB3 as a promising non-invasive biomarker for type 2 diabetes and diabetic nephropathy","authors":"Seyed Amirhossein Hosseini , Parisa Ajorlou , Hasti Haddadian , Shahla Sohrabipour","doi":"10.1016/j.ab.2025.115965","DOIUrl":"10.1016/j.ab.2025.115965","url":null,"abstract":"<div><div>The prevalence of Type 2 diabetes mellitus (T2DM) is increasing worldwide and represents a major risk factor for the development of diabetic nephropathy (DN), a severe microvascular complication. Chronic hyperglycemia activates inflammatory and fibrotic signaling pathways, which contribute to kidney damage. Integrins, as transmembrane adhesion receptors, play pivotal roles in regulating inflammation, immune cell trafficking, and insulin resistance. This research focused on identifying non-invasive biomarkers for T2DM and DN using PBMCs. Differentially expressed genes related to diabetes were identified through the analysis of multiple datasets retrieved from the Gene Expression Omnibus, including <span><span>GSE95849</span><svg><path></path></svg></span>, <span><span>GSE9006</span><svg><path></path></svg></span>, <span><span>GSE25724</span><svg><path></path></svg></span>, and <span><span>GSE159984</span><svg><path></path></svg></span>. ITGB3 was identified as a common gene across these datasets, and its expression in DN was further examined using the <span><span>GSE142025</span><svg><path></path></svg></span> dataset. Real-time PCR analysis of PBMC samples revealed a significant upregulation of ITGB3 expression in individuals with DN and T2DM compared to healthy controls. The TF2DNA and miRNASNPv3 databases identified 10 transcription factors and 10 variants of ITGB3 involved in 60 miRNA interactions. Additionally, the DGIdb database revealed 15 drugs potentially regulating ITGB3 expression. These findings underscore the importance of integrin-related pathways in diabetes and suggest ITGB3 as a promising target for future research and therapeutic development.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"707 ","pages":"Article 115965"},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel dual-function sustainable method for H2O2 determination and dye biomineralization utilizing peroxidase-like MnFe2O4@CrFe2O4 nanocomposite 利用过氧化物酶样MnFe2O4@CrFe2O4纳米复合材料的新型双功能可持续的H2O2测定和染料生物矿化方法

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2025-08-22 DOI: 10.1016/j.ab.2025.115962

Saeed Reza Hormozi Jangi , Masood Ranjoori , Anahita Barghi , Pardis Ahmadi

{"title":"A novel dual-function sustainable method for H2O2 determination and dye biomineralization utilizing peroxidase-like MnFe2O4@CrFe2O4 nanocomposite","authors":"Saeed Reza Hormozi Jangi , Masood Ranjoori , Anahita Barghi , Pardis Ahmadi","doi":"10.1016/j.ab.2025.115962","DOIUrl":"10.1016/j.ab.2025.115962","url":null,"abstract":"<div><div>In this contribution, a novel dual-function sustainable green nanozyme-mediated method for highly sensitive and selective H<sub>2</sub>O<sub>2</sub> quantification and reusable rhodamine B biomineralization utilizing highly active MnFe<sub>2</sub>O<sub>4</sub>@CrFe<sub>2</sub>O<sub>4</sub> nanocomposite with synergistic peroxidase-like activity was designed and developed. This method also introduced a sustainable approach for probing the analyte instead of exploiting prevalent carcinogenic nanozyme-based analytical probes, making it absolutely more sustainable than the conventional methods. The hydrogen peroxide biosensor acquired a linear range of 1–100 μM and a very low detection limit of 0.6 μM, along with an inter-day %RSD of 2.74 % and a highly selective response against coexisting materials. Ultimately, the sensor was employed for H<sub>2</sub>O<sub>2</sub> quantification in milk, revealing a recovery of 96.1–102.8 %, %RSD = 1.4–3.6 %. Besides, the effective factors on decolorization yield were optimized, providing a high biomineralization yield of 99.4 % at optimal experimental conditions within a short time of 35.0 min. The breakthrough volume, storage stability, and reusability of the nanozymes were assessed, revealing a breakthrough volume of 5.0–1000 mL, a shelf-life of 20 days, and 70 % yield saving after 10 cycles. The method was applied for dye degradation in real water media, including river water, pool water, and tap water, revealing a high yield of over 95.4–99.5 %, %RSD = 1.8–4.2 %.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"707 ","pages":"Article 115962"},"PeriodicalIF":2.5,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0