Analytical biochemistry最新文献

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An overview of biosensor advancements for detecting botulinum neurotoxins: Addressing food safety and biowarfare risks 用于检测肉毒杆菌神经毒素的生物传感器进展综述:解决食品安全和生物战风险。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-05 DOI: 10.1016/j.ab.2025.115801
Arzoo Saini , Neelam Yadav , Bijender Singh , Jogender Singh Rana
{"title":"An overview of biosensor advancements for detecting botulinum neurotoxins: Addressing food safety and biowarfare risks","authors":"Arzoo Saini ,&nbsp;Neelam Yadav ,&nbsp;Bijender Singh ,&nbsp;Jogender Singh Rana","doi":"10.1016/j.ab.2025.115801","DOIUrl":"10.1016/j.ab.2025.115801","url":null,"abstract":"<div><div>Botulinum neurotoxins (BoNTs) are lethal toxins produced by bacteria <em>Clostridium botulinum</em>. Ingestion of BoNTs contaminated foods causes botulism which affects individual's nervous system by blocking the release of neurotransmitters causing flaccid paralysis. This review article deciphers the comprehensive account on mechanism of action of BoNTs, pathogenicity, and various innovative analytical detection techniques of BoNTs in foods. Potential misuse of BoNT as a biowarfare agent is also a major concern. Hence, for the detection of deadly BoNTs various conventional techniques like mouse lethality bioassay (MLB), SNAP-25 assay, mouse phrenic nerve hemidiaphragm (MPN) test, non-lethal mouse flaccid paralysis assay (NFPA) and modern techniques (immunoassays, cell-based assay, nucleic acid-based methods, endopeptidase mass spectrometry assays) have been discussed. This article also provides a detailed account on biosensing technology for detecting BoNTs in foods. Moreover, future research efforts should be focused on the development of advanced new-age biosensors for automated detection and real time monitoring of botulinum neurotoxin toxicity in food. Integration of biosensors with quantum technology and lab-on-chip platforms is required for increasing their versatility and robust detection. The insights presented in the review aim towards providing future research directions and increase the vigilance against potential future threats.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115801"},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aptasensor based on rGO-AuNPs Field-effect Transistor for selective detection of Escherichia coli in river water 基于rGO-AuNPs场效应晶体管的感应传感器选择性检测河水中大肠杆菌
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-05 DOI: 10.1016/j.ab.2025.115796
Hale Alvandi , Ali Hossein Rezayan , Hassan Hajghassem , Fereshteh Rahimi , Reza Askari Moghadam , Ali Firoozbakhtian
{"title":"Aptasensor based on rGO-AuNPs Field-effect Transistor for selective detection of Escherichia coli in river water","authors":"Hale Alvandi ,&nbsp;Ali Hossein Rezayan ,&nbsp;Hassan Hajghassem ,&nbsp;Fereshteh Rahimi ,&nbsp;Reza Askari Moghadam ,&nbsp;Ali Firoozbakhtian","doi":"10.1016/j.ab.2025.115796","DOIUrl":"10.1016/j.ab.2025.115796","url":null,"abstract":"<div><div>Water and food-borne diseases are public health problems. It is estimated that only water-borne diseases cause 2.2 million deaths annually. <em>E. coli</em> is one of the most important bacteria in water monitoring and is on the WHO's list of priority pathogens for diagnosis and treatment. Conventional methods for detecting <em>E. coli</em> are not effective due to their time-consuming nature, the need for expensive equipment, and low sensitivity. Therefore, a rapid diagnostic method is essential for accurate detection of <em>E. coli</em>. Here, a Field-effect Transistor (FET) was used to detect <em>E. coli</em> based on rGO, AuNPs, and ssDNA-aptamer. After characterizing the rGO-AuNPs-Apt FET, the current of the nanobiosensor was measured with each modification. The nanobiosensor's linear range was (3–3 × 10<sup>6</sup> CFU/ml), and LOD reached 3 CFU/ml in the PBS buffer. The nanobiosensor's response was completely selective and stable for up to 4 weeks. The rGO-AuNPs-Apt FET specifically detected <em>E. coli</em> in the river water down to 10 CFU/ml, even in a mixture of other bacteria at higher concentrations. The small sample size, ease of use, and accuracy of detection are the advantages of rGO-AuNPs-Apt FET, which can be used as a sensor for water monitoring in 15 min.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115796"},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-depth characterization and semi-quantification of anti-drug antibodies in clinical samples using specific hybrid IC-LC-MS/MS methods 使用特异性混合IC-LC-MS/MS方法对临床样品中抗药物抗体进行深入表征和半定量。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-05 DOI: 10.1016/j.ab.2025.115797
Stéphane Muccio , Christophe Hirtz , Daniel Kramer , Johanna Paris , Sandrine Descloux , Olivier Fedeli , Annemie Deiteren , Anastasia Tribula , Sylvain Lehmann , Jérôme Vialaret
{"title":"In-depth characterization and semi-quantification of anti-drug antibodies in clinical samples using specific hybrid IC-LC-MS/MS methods","authors":"Stéphane Muccio ,&nbsp;Christophe Hirtz ,&nbsp;Daniel Kramer ,&nbsp;Johanna Paris ,&nbsp;Sandrine Descloux ,&nbsp;Olivier Fedeli ,&nbsp;Annemie Deiteren ,&nbsp;Anastasia Tribula ,&nbsp;Sylvain Lehmann ,&nbsp;Jérôme Vialaret","doi":"10.1016/j.ab.2025.115797","DOIUrl":"10.1016/j.ab.2025.115797","url":null,"abstract":"<div><div>Anti-drug antibodies (ADAs) generated by biotherapeutics can impair the drug clearance, prevent the binding to its target or lead to hypersensitivity reactions, thereby affecting efficacy and safety. It is therefore essential to assess the immunogenicity of potential biotherapeutics, particularly in clinical development. Ligand binding assays (LBA) are the gold standard for ADA detection because of their high sensitivity and throughput. However, LBA assays don't provide details on the isotypes produced and their relative abundance. As certain isotypes are known to be associated with ADA mediated adverse events, this information could be helpful to anticipate or better characterize the immunogenicity risk of biotherapeutics. A hybrid IC-LC-MS/MS strategy was developed for the detection of specific isotypes/subclasses of ADAs in a phase I clinical study. A first approach using the biotinylated drug to capture ADAs in human serum allowed the simultaneous semi-quantification of all IgG subclasses and the detection of ADAs of the IgM isotype in clinical samples. These results enabled a detailed characterization of the immune response against the biotherapeutic. A second assay was developed using a sequential immunocapture to measure drug specific IgEs known to be potentially associated with hypersensitivity reactions. The overall results were consistent with the clinical adverse events observed in some healthy volunteers.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115797"},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Green synthesized Ag-nanoparticles using Scutellaria multicaulis stem extract and their selective cytotoxicity against breast cancer” [Anal. Biochem. (653) (2022) 114786] “使用黄芩茎提取物的绿色合成银纳米颗粒及其对乳腺癌的选择性细胞毒性”的勘误表[肛门]。物化学。[653][2022][114786]。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-04 DOI: 10.1016/j.ab.2025.115790
Zahra Gharari , Parichehr Hanachi , Tony R. Walker
{"title":"Corrigendum to “Green synthesized Ag-nanoparticles using Scutellaria multicaulis stem extract and their selective cytotoxicity against breast cancer” [Anal. Biochem. (653) (2022) 114786]","authors":"Zahra Gharari ,&nbsp;Parichehr Hanachi ,&nbsp;Tony R. Walker","doi":"10.1016/j.ab.2025.115790","DOIUrl":"10.1016/j.ab.2025.115790","url":null,"abstract":"","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115790"},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantification of amyloid-β aggregation inhibitors gallic acid and rosmarinic acid in biological samples by LC-MS/MS LC-MS/MS定量测定生物样品中淀粉样蛋白-β聚集抑制剂没食子酸和迷迭香酸的含量
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-04 DOI: 10.1016/j.ab.2025.115799
Raluca Ştefănescu
{"title":"Quantification of amyloid-β aggregation inhibitors gallic acid and rosmarinic acid in biological samples by LC-MS/MS","authors":"Raluca Ştefănescu","doi":"10.1016/j.ab.2025.115799","DOIUrl":"10.1016/j.ab.2025.115799","url":null,"abstract":"<div><div>The decline of the cognitive functions encountered at patients diagnosed with Alzheimer's disease (AD) together with the findings of extracellular amyloid deposits, intracellular neurofibrillary tangles and microvascular angiopathy in brain were described at beginning of the 20th century. According to the amyloid cascade hypothesis, the overproduction of amyloid-β peptide and its aggregation into neurotoxic oligomers, fibrils, and amyloid plaques is considered the cause of AD. Amyloid-β fibril formation was experimentally proven <em>in vitro</em> using thioflavin T assay in the absence of interfering chemical compounds and the assay became an analytical tool for assessing the effects of different molecules against amyloid-β aggregation. Recent research studies provided experimental results that indicated the reduction of fibril formation by gallic acid and rosmarinic acid. Mass spectrometry was often employed in studies aiming at identifying, characterizing, and quantitating chemical compounds able to modify the progress of AD. The purpose of this review is to present current research studies regarding the identification and quantitation of the water-soluble gallic acid and rosmarinic acid in biological samples using liquid chromatographs coupled to triple quadrupole mass spectrometers as bioanalytical tools. The present study highlights the presence and amount of these chemical compounds in commonly used medicinal plants and culinary herbs and provides a list of extraction and liquid chromatography coupled to electrospray-triple quadrupole mass spectrometry methods examples described in previous pharmacokinetic studies. The article underlines the bioavailability and safety of gallic acid and rosmarinic acid for further research studies aiming at preventing and slowing the progress of AD.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115799"},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143221550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spectrofluorimetric and theoretical investigation of a glucocorticoid drug with HSA: Quantitative studies in real sample 一种糖皮质激素药物的HSA荧光光谱法和理论研究:实际样品的定量研究
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-04 DOI: 10.1016/j.ab.2025.115800
Nabila Khalil, Adila Khalil, Mohammad Kashif
{"title":"Spectrofluorimetric and theoretical investigation of a glucocorticoid drug with HSA: Quantitative studies in real sample","authors":"Nabila Khalil,&nbsp;Adila Khalil,&nbsp;Mohammad Kashif","doi":"10.1016/j.ab.2025.115800","DOIUrl":"10.1016/j.ab.2025.115800","url":null,"abstract":"<div><div>For the assay of methylprednisolone (MP) in pharmaceutical formulation, a fluorescence-based spectroscopic approach is developed using the protein, human serum albumin (HSA). The structural alterations in human serum albumin (HSA) following its interaction with methylprednisolone (MP) (10–32 μg mL<sup>−1</sup>) were determined by spectroscopy, which included the use of fluorescence and synchronous fluorescence. The calibration curve was modeled using the fluorescence method. The devised approach has a limit of detection of 1.16 μg mL<sup>−1</sup> and a limit of quantification of 3.535 μg mL<sup>−1</sup> for MP estimation that is straightforward, sensitive, accurate, and selective. The reference approach and the suggested method were contrasted to show which was more appropriate for MP quality control in its dosage forms. The recovery data obtained from 99.63% to 100.0% for intra-day and 99.60–100.1%, for inter-day precision. The developed method showed remarkable sensitivity to dosage forms and real sample (urine), suggesting possible application, when applied to dose forms with a relative standard deviation of less than 2%.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115800"},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-resolution repeat structure analysis in myotonic dystrophy type 2 diagnostics using short-read whole genome sequencing 短读全基因组测序在2型肌强直性营养不良诊断中的高分辨率重复结构分析。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.ab.2025.115793
Ingrid Lojova , Marcel Kucharik , Andrea Zatkova , Andrej Balaz , Zuzana Pös , Eva Tothova Tarova , Ludevit Kadasi , Jaroslav Budis , Tomas Szemes , Jan Radvanszky
{"title":"High-resolution repeat structure analysis in myotonic dystrophy type 2 diagnostics using short-read whole genome sequencing","authors":"Ingrid Lojova ,&nbsp;Marcel Kucharik ,&nbsp;Andrea Zatkova ,&nbsp;Andrej Balaz ,&nbsp;Zuzana Pös ,&nbsp;Eva Tothova Tarova ,&nbsp;Ludevit Kadasi ,&nbsp;Jaroslav Budis ,&nbsp;Tomas Szemes ,&nbsp;Jan Radvanszky","doi":"10.1016/j.ab.2025.115793","DOIUrl":"10.1016/j.ab.2025.115793","url":null,"abstract":"<div><h3>Background/Objectives</h3><div>Diagnostic possibilities for myotonic dystrophy type 2 (DM2) are constantly evolving in order to achieve more accurate and faster diagnosis. Whole genome sequencing (WGS), together with specialized tandem repeat (TR) genotyping bioinformatic tools, represent a breakthrough technology in molecular diagnostics. We decided to characterize new opportunities and challenges in WGS-based DM2 molecular diagnostics.</div></div><div><h3>Methods</h3><div>WGS data were obtained from 50 individuals, including five DM2 patients, and one individual carrying a premutation range allele. TR characterization was performed using a modified version of the Dante tool, with results validated by conventional PCR and repeat-primed PCR.</div></div><div><h3>Results</h3><div>We used WGS to identify all of the expansion-range DM2 alleles, together with the premutation-range allele. Compared to conventional methods, WGS was more efficient for a detailed sequence structure characterization of the normal-range alleles, and phasing of the entire CNBP-complex motif. A 97 % genotyping concordance rate was achieved between the conventional methods and the WGS-derived results, with discrepancies mainly based on single-repeat differences in the genotypes. The stutter effect introduced some uncertainty in both methods.</div></div><div><h3>Conclusion</h3><div>Short-read WGS offers significant potential for DM2 diagnostics by enabling precise repeat motif characterization and may also apply to other tandem repeat disorders (TRDs).</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115793"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Next-generation electrochemical biosensors for acrylamide: Progress, challenges, and opportunities 下一代丙烯酰胺电化学生物传感器:进展、挑战和机遇。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115798
Shatrughan Aafria, Minakshi Sharma
{"title":"Next-generation electrochemical biosensors for acrylamide: Progress, challenges, and opportunities","authors":"Shatrughan Aafria,&nbsp;Minakshi Sharma","doi":"10.1016/j.ab.2025.115798","DOIUrl":"10.1016/j.ab.2025.115798","url":null,"abstract":"<div><div>Acrylamide is a hazardous substance present in heat-processed food products and industrial wastewater. It is carcinogenic and neurotoxic and therefore emphasises the importance of monitoring its levels and the need for sensitive and accurate detection techniques. Electrochemical biosensing has emerged as a potential analytical method for detecting acrylamide. This article provides a comprehensive overview of the most recent developments in electrochemical biosensing methods, including amperometric, potentiometric, and impedimetric biosensors for acrylamide detection. The creation and use of novel biorecognition components, such as enzymes, antibodies, and molecularly imprinted polymers that enhance the sensitivity and specificity of acrylamide monitoring, are given special attention. Incorporating nanomaterials such as carbon-based nanomaterials and metallic nanoparticles was investigated for its potential to improve the sensors' electrochemical characteristics and overall efficacy. The potential of electrochemical biosensors for acrylamide detection is further illustrated, showcasing their effectiveness in a range of matrices in different food products. This review aims to inform researchers about the latest technological developments, trends, and future directions in electrochemical biosensing for acrylamide detection. The study highlights the significance of ongoing research and cooperation in creating efficient biosensing systems to protect public health and the environment by thoroughly examining current technology and pointing out areas for improvement.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115798"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemical flow injection analysis platforms for biomedical applications – Progress and prospects 生物医学应用的电化学流动注射分析平台。进展与展望。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115795
Subramanian Nellaiappan , Nandimalla Vishnu , Devaraj Manoj , Thamaraiselvi Kanagaraj , Manickam Selvaraj , Mohammed A. Assiri
{"title":"Electrochemical flow injection analysis platforms for biomedical applications – Progress and prospects","authors":"Subramanian Nellaiappan ,&nbsp;Nandimalla Vishnu ,&nbsp;Devaraj Manoj ,&nbsp;Thamaraiselvi Kanagaraj ,&nbsp;Manickam Selvaraj ,&nbsp;Mohammed A. Assiri","doi":"10.1016/j.ab.2025.115795","DOIUrl":"10.1016/j.ab.2025.115795","url":null,"abstract":"<div><div>Flow injection analysis coupled with electrochemical detection (FIA-ECD) techniques has to be a key technological development for wide variety of applications in various domains such as synthetic analytical chemistry, materials science, biomedical and point-of-care devices. FIA-ECD techniques have to be unique due to decreasing the time of analysis, sample pre-treatment, reactions and separation-free detection for multi-analytes. In biomedical domain, various electrochemical sensors were decorated with bioactive molecules, i.e., DNA, antibodies, enzymes along with biocompatible polymers for the detection of biologically important chemicals and analytes. The flexibility and practical applicability of these sensors may serve as a new pattern for clinical and biomedical analytical instrumentations. This review covers the highlights and overview of the most successful and promising electrochemical sensors, biosensors and immunosensors integrated with the flow injection analysis platforms, fabrication and analytical parameters, pros and cons of these sensor technologies, challenges and future opportunities in point-of-care clinical analysis.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115795"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Total flavonoid content revised: An overview of past, present, and future determinations in phytochemical analysis 总黄酮含量修订:植物化学分析中过去、现在和未来测定的概述。
IF 2.6 4区 生物学
Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115794
Alexandru Nicolescu , Claudiu Ioan Bunea , Andrei Mocan
{"title":"Total flavonoid content revised: An overview of past, present, and future determinations in phytochemical analysis","authors":"Alexandru Nicolescu ,&nbsp;Claudiu Ioan Bunea ,&nbsp;Andrei Mocan","doi":"10.1016/j.ab.2025.115794","DOIUrl":"10.1016/j.ab.2025.115794","url":null,"abstract":"<div><div>Flavonoids represent an important research topic in the analytical chemistry of secondary plant metabolites. During habitual laboratory determinations, preliminary quantitative analysis is often associated with <em>in vitro</em> colorimetric assessment. Total flavonoid content (TFC) is used as screening method with high relevance in the chemical analysis of plants and derived products, being typically applied before HPLC-MS phytochemical profiling. Its importance stems from affordability, simplicity, rapidity, and low cost. The AlCl<sub>3</sub> assay, with or without NaNO<sub>2</sub> addition, is the most used method in the present, although less frequently used methods (using 2,4-dinitrophenylhydrazine, dimethylamino-cinnamaldehyde, or diethylene glycol) show potential for complementary and specific determinations. Given the prevalence of research papers focusing on a single method for “total flavonoid” determination, we identified the need for an objective and critical comparison of existing methodologies. Moreover, a special notice is dedicated to the past and the future of <em>in vitro</em> TFC determinations, in the context of recent advances in flavonoid research. The focal point of this review is to serve as a basis for laboratory protocol reorganization regarding TFC determination, as a powerful tool before mass spectrometry, as well as to present a potential complementary analysis protocol applicable to biological samples. Among the methods found in the literature, SBC was the only assay providing accurate determinations of TFC.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115794"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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