Analytical biochemistry最新文献_第9页

Small molecule substrates for the rapid quantification of acyl transfer activity of nylon hydrolase NylCA 用于快速定量尼龙水解酶 NylCA 的酰基转移活性的小分子底物。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-02 DOI: 10.1016/j.ab.2024.115598

Alana M.M. Rangaswamy , Francis M. Roy , Jeffrey W. Keillor

{"title":"Small molecule substrates for the rapid quantification of acyl transfer activity of nylon hydrolase NylCA","authors":"Alana M.M. Rangaswamy , Francis M. Roy , Jeffrey W. Keillor","doi":"10.1016/j.ab.2024.115598","DOIUrl":"10.1016/j.ab.2024.115598","url":null,"abstract":"<div><p>The widespread use of polyamides such as nylons has led to the accumulation of nylon waste, which is particularly resistant to decomposition due to the intrinsic stability of the amide bond. New methods are required for the true recycling of these waste materials by depolymerization. Enzymes that are capable of hydrolyzing polyamides have been proposed as biocatalysts that may be suitable for this application. NylC is an enzyme that can mediate the hydrolysis of aminohexanoic acid oligomers, and to some extent, bulk polymers. However, current assays to characterize the activity of this enzyme require long reaction times and/or rely on secondary reactions to quantify hydrolysis. Herein, we have designed structurally-optimized small molecule chromogenic esters that serve as substrate analogues for monitoring NylC acyltransferase activity in a continuous manner. This assay can be performed in minutes at room temperature, and the substrate <em>N</em>-acetyl-GABA-pNP ester (<em>k</em><sub>cat</sub> = 0.37 s<sup>−1</sup>, <em>K</em><sub>M</sub> = 256 μM) shows selectivity for NylC in complex biological media. We also demonstrate that activity towards this substrate analogue correlates with amide hydrolysis, which is the primary activity of this enzyme. Furthermore, our screening of substrate analogues provides insight into the substrate specificity of NylC, which is relevant to biocatalytic applications.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Customizable BAC-based DNA markers for pulsed-field gel electrophoresis 用于脉冲场凝胶电泳的基于 BAC 的可定制 DNA 标记。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-06-25 DOI: 10.1016/j.ab.2024.115596

Yin Cheng Wong , Allan Wee Ren Ng , Andrew Osahor , Kumaran Narayanan

引用次数: 0

Electrochemical detection of nalbuphine drug using oval-like ZnO nanostructure-based sensor 利用基于椭圆形氧化锌纳米结构的传感器对纳布啡药物进行电化学检测

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-06-21 DOI: 10.1016/j.ab.2024.115595

Kanwal Hussain , Rafiq Ahmad , Sohail Hassan , Muhammad Y. Khan , Akil Ahmad , Mohammed B. Alshammari , Muhammad S. Ali , Saeed A. Lakho , Byeong-Il Lee

{"title":"Electrochemical detection of nalbuphine drug using oval-like ZnO nanostructure-based sensor","authors":"Kanwal Hussain , Rafiq Ahmad , Sohail Hassan , Muhammad Y. Khan , Akil Ahmad , Mohammed B. Alshammari , Muhammad S. Ali , Saeed A. Lakho , Byeong-Il Lee","doi":"10.1016/j.ab.2024.115595","DOIUrl":"10.1016/j.ab.2024.115595","url":null,"abstract":"<div><p>Monitoring pharmaceutical drugs in various mediums is crucial to mitigate adverse effects. This study presents a chemical sensor using an oval-like zinc oxide (ZnO) nanostructure for electrochemical detection of nalbuphine. The ZnO nanostructure, produced via an efficient sol-gel technique, was extensively characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), UV–visible spectrophotometry, and fourier transform infrared spectroscopy (FTIR). A slurry of the ZnO nanostructure in a binder was applied to a glassy carbon electrode (GCE). The sensor's responsiveness to nalbuphine was assessed using linear sweep voltammetry (LSV), achieving optimal performance by fine-tuning the pH. The sensor demonstrated a proportional response to nalbuphine concentrations up to 150.0 nM with a good regression coefficient (R<sup>2</sup>) and a detection limit of 6.20 nM (S/N ratio of 3). Selectivity was validated against various interfering substances, and efficacy was confirmed through real sample analysis, highlighting the sensor's successful application for nalbuphine detection.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Palindrome sequence mediated target recycling integrating with self-priming assisted signal reaction for sensitive miRNA detection 由 Palindrome 序列介导的目标再循环与自吸辅助信号反应相结合，用于灵敏的 miRNA 检测。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-06-17 DOI: 10.1016/j.ab.2024.115594

Linling Xu , Fengrong Yuan , Ling Wang , Ting Peng

{"title":"Palindrome sequence mediated target recycling integrating with self-priming assisted signal reaction for sensitive miRNA detection","authors":"Linling Xu , Fengrong Yuan , Ling Wang , Ting Peng","doi":"10.1016/j.ab.2024.115594","DOIUrl":"10.1016/j.ab.2024.115594","url":null,"abstract":"<div><p>The development of a sensitive and isothermal technique with a greatly enhanced miRNA detection signal is still technically problematic due to the low abundance of miRNA and high sequence similarities with homologous miRNAs. Herein, we propose a novel fluorescence approach for sensitive and reliable miRNA detection by integrating the palindrome sequence mediated target recycling with self-priming assisted signal reaction. In this method, a dual toehold DNA nano-probe (HT) with two functional arms is designed to mediate specific target recognition and signal amplification. In the presence of target miRNA, it binds to the recognition module of HT probe, releasing the “2” sequence to initiate strand displacement amplification (SDA) and a self-priming-induced signal reaction. Based on the elegant design, the proposed method exhibits a wide linear response range exceeding five orders of magnitude and a low limit of detection of 0.96 fM according to the 3δ rule. The non-specific signal is below 5 % for non-target miRNA detection. Taking the merits of excellent sensitivity, desirable specificity, and superior anti-interference ability, the proposed approach shows a promising prospect for detecting miRNAs in complicated biological environments and early diagnosis of diseases.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target recycle initiated entropy driven assembly strategy for sensitive, enzyme-free, and portable miRNA detection 用于灵敏、无酶和便携式 miRNA 检测的目标循环启动熵驱动组装策略

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-06-15 DOI: 10.1016/j.ab.2024.115593

Zhijun Jiang, Zhiyuan Liu

{"title":"Target recycle initiated entropy driven assembly strategy for sensitive, enzyme-free, and portable miRNA detection","authors":"Zhijun Jiang, Zhiyuan Liu","doi":"10.1016/j.ab.2024.115593","DOIUrl":"https://doi.org/10.1016/j.ab.2024.115593","url":null,"abstract":"<div><p>MicroRNA (miRNA) is a pivotal biomarker in the diagnosis of various cancers, including bladder cancer (BCa). Despite their significance, the low abundance of miRNA presents a substantial challenge for sensitive and reliable detection. We introduce an innovative, highly sensitive assay for miRNA expression quantification that is both enzyme-free and portable. This method leverages the synergy of target recycling and entropy-driven assembly (EDA) for enhanced sensitivity and specificity. The proposed method possesses several advantages, including i) dual signal amplification through target recycling and EDA, which significantly boosts sensitivity with a lower limit of detection of 2.54 fM; ii) elimination of enzyme requirements, resulting in a cost-effective and stable signal amplification process; and iii) utilization of a personal glucose meter (PGM) for signal recording, rendering the method portable and adaptable to diverse settings. In summary, this PGM-based approach holds promising potential for clinical molecular diagnostics, offering a practical and efficient solution for miRNA analysis in cancer detection.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of MicroRNA-155 based on lambda exonuclease selective digestion and CRISPR/cas12a-assisted amplification 基于 Lambda 外切酶选择性消化和 CRISPR/Cas12a 辅助扩增的 MicroRNA-155 检测。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-06-11 DOI: 10.1016/j.ab.2024.115592

Haotian Zhang , Jun Xu , Shiwen Liu , Hongbo Li , Lianlian Xu , Suqin Wang

{"title":"Detection of MicroRNA-155 based on lambda exonuclease selective digestion and CRISPR/cas12a-assisted amplification","authors":"Haotian Zhang , Jun Xu , Shiwen Liu , Hongbo Li , Lianlian Xu , Suqin Wang","doi":"10.1016/j.ab.2024.115592","DOIUrl":"10.1016/j.ab.2024.115592","url":null,"abstract":"<div><p>In numerous malignancies, miRNA-155 is overexpressed and has oncogenic activity because it is one of the most efficient microRNAs for inhibiting apoptosis in human cancer cells. As a result, the highest sensitive detection of the miRNA-155 gene is a technological instrument that can enable early cancer screening. In this study, a miRNA-155 biosensor was created to create a hairpin probe that can bind to the miRNA-155 gene using lambda nucleic acid exonuclease, which can cut the 5′ phosphorylated double strand, and by the DNA probe is recognized by the Cas12a enzyme, which then activates Cas12a to catalyze <em>trans</em>-cutting produces strong fluorescence. Research finding, the target concentration's logarithm and corresponding fluorescence intensity have a strong linear connection, and the limit of detection (LOD) of the sensing system was determined to be 8.3 pM. In addition, the biosensor displayed exceptional specificity, low false-positive signal, and high sensitivity in detecting the miRNA-155 gene in serum samples. This study's creation of a biosensor that has high sensitivity, good selectivity, and is simple to operate provides promising opportunities for research into biosensor design and early cancer detection.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of a LC-MS based multi-attribute method (MAM) and intact multi-attribute method (iMAM) workflow for the characterisation of a GLP-Fc fusion protein 实施基于 LC-MS 的多属性方法 (MAM) 和完整多属性方法 (iMAM) 工作流程，以表征 GLP-Fc 融合蛋白。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-06-06 DOI: 10.1016/j.ab.2024.115585

Ciarán Buckley , Silvia Millán-Martín , Sara Carillo , Florian Füssl , Ciara MacHale , Jonathan Bones

{"title":"Implementation of a LC-MS based multi-attribute method (MAM) and intact multi-attribute method (iMAM) workflow for the characterisation of a GLP-Fc fusion protein","authors":"Ciarán Buckley , Silvia Millán-Martín , Sara Carillo , Florian Füssl , Ciara MacHale , Jonathan Bones","doi":"10.1016/j.ab.2024.115585","DOIUrl":"10.1016/j.ab.2024.115585","url":null,"abstract":"<div><p>Over the past few years, the implementation of mass spectrometry (MS) in QC laboratories has become a more common occurrence. The multi-attribute method (MAM), and emerging intact multi-attribute method (iMAM), are powerful analytical tools utilising liquid chromatography−mass spectrometry (LC-MS) methods that enable the monitoring of critical quality attributes (CQAs) in biotherapeutic proteins in compliant settings. Both MAM and iMAM are intended to replace or supplement several conventional assays with a single LC-MS method utilising MS data in combination with robust, semi-automated data processing workflows. MAM and iMAM workflows can also be implemented into current Good Manufacturing Practices environments due to the availability of CFR 11 compliant chromatography data system software. In this study, MAM and iMAM are employed for the analysis of 4 batches of a glucagon-like peptide-Fc fusion protein. MAM approach involved a first the discovery phase for the identification of CQAs and second, the target monitoring phase of the selected CQAs in other samples<em>.</em> New peak detection was performed on the data set to determine the appearance, absence or change of any peak. For native iMAM workflow both size exclusion and strong cation exchange chromatography were optimized for the identification and monitoring of CQAs at the intact level.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003269724001295/pdfft?md5=e0ca18d3439a327f787b3a911c30949e&pid=1-s2.0-S0003269724001295-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational design and application of broad-spectrum antibodies for Bt Cry toxins determination 用于测定 Bt Cry 毒素的广谱抗体的合理设计和应用

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-06-04 DOI: 10.1016/j.ab.2024.115584

Jiafeng Jin , Wei Chen , Chongxin Xu , Ofentse Jacob Pooe , Yajing Xie , Cheng Shen , Meng Meng , Qin Zhu , Xiao Zhang , Xianjin Liu , Yuan Liu

{"title":"Rational design and application of broad-spectrum antibodies for Bt Cry toxins determination","authors":"Jiafeng Jin , Wei Chen , Chongxin Xu , Ofentse Jacob Pooe , Yajing Xie , Cheng Shen , Meng Meng , Qin Zhu , Xiao Zhang , Xianjin Liu , Yuan Liu","doi":"10.1016/j.ab.2024.115584","DOIUrl":"10.1016/j.ab.2024.115584","url":null,"abstract":"<div><p>Using the amino acid sequences and analysis of selected known structures of Bt Cry toxins, Cry1Ab, Cry1Ac, Cry1Ah, Cry1B, Cry1C and Cry1F we specifically designed immunogens. After antibodies selection, broad-spectrum polyclonal antibodies (pAbs) and monoclonal antibody (namely 1A0-mAb) were obtained from rabbit and mouse, respectively. The produced pAbs displayed broad spectrum activity by recognizing Cry1 toxin, Cry2Aa, Cry2Ab and Cry3Aa with half maximal inhibitory concentration (IC<sub>50</sub>) values of 0.12–9.86 μg/mL. Similarly, 1A0-mAb showed broad spectrum activity, recognizing all of the above Cry protein (IC<sub>50</sub> values of 4.66–20.46 μg/mL) with the exception of Cry2Aa. Using optimizations studies, 1A10-mAb was used as a capture antibody and pAbs as detection antibody. Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) were established for Cry1 toxin, Cry2Ab and Cry3Aa with the limit of detection (LOD) values of 2.36–36.37 ng/mL, respectively. The present DAS-ELISAs had good accuracy and precisions for the determination of Cry toxin spiked tap water, corn, rice, soybeans and soil samples. In conclusion, the present study has successfully obtained broad-spectrum pAbs and mAb. Furthermore, the generated pAbs- and mAb-based DAS-ELISAs protocol can potentially be used for the broad-spectrum monitoring of eight common subtypes of Bt Cry toxins residues in food and environmental samples.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141278867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic paper-based chemiluminescence sensing platform based on functionalized CaCO3 for time-resolved multiplex detection of avian influenza virus biomarkers 基于功能化 CaCO3 的微流纸基化学发光传感平台，用于禽流感病毒生物标记物的时间分辨多重检测。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-06-04 DOI: 10.1016/j.ab.2024.115583

Yafei Tian, Yujiao Zhang, Xueyun Lu, Dan Xiao, Cuisong Zhou

{"title":"Microfluidic paper-based chemiluminescence sensing platform based on functionalized CaCO3 for time-resolved multiplex detection of avian influenza virus biomarkers","authors":"Yafei Tian, Yujiao Zhang, Xueyun Lu, Dan Xiao, Cuisong Zhou","doi":"10.1016/j.ab.2024.115583","DOIUrl":"10.1016/j.ab.2024.115583","url":null,"abstract":"<div><p>Multiplex detection can enhance diagnostic precision and improve diagnostic efficiency, providing important assistance for epidemiological investigation and epidemic prevention. There is a great need for multi-detection sensing platforms to accurately diagnose diseases. Herein, we reported a μPAD-based chemiluminescence (CL) assay for ultrasensitive multiplex detection of AIV biomarkers, based on three DNAzyme/Lum/PEI/CaCO<sub>3</sub>. Three time-resolved CL signals were sequentially generated with detection limits of 0.32, 0.34, and 0.29 pM for H1N1, H7N9, and H5N1, respectively, and with excellent selectivity against interfering DNA. The recovery test in human serum displayed satisfactory analysis capabilities for complex biological samples. The μPAD-based CL assay achieved multiplex detection within 70 s, with a high time resolution of 20 s. The proposed strategy has the advantages of low cost, high sensitivity, good selectivity, and wide time resolution, the μPAD-based CL assay has shown great potential in the early and accurate diagnosis of diseases.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of sterile platform for quantification of extracellular analytes via single walled carbon nanotubes 开发通过单壁碳纳米管定量检测细胞外分析物的无菌平台

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-31 DOI: 10.1016/j.ab.2024.115582

Ivon Acosta-Ramirez , Carley Conover , Jacob Larsen , Portia N.A. Plange , Ufuk Kilic , Becca Muller , Nicole M. Iverson

{"title":"Development of sterile platform for quantification of extracellular analytes via single walled carbon nanotubes","authors":"Ivon Acosta-Ramirez , Carley Conover , Jacob Larsen , Portia N.A. Plange , Ufuk Kilic , Becca Muller , Nicole M. Iverson","doi":"10.1016/j.ab.2024.115582","DOIUrl":"10.1016/j.ab.2024.115582","url":null,"abstract":"<div><p>Progress has been made studying cell-cell signaling communication processes. However, due to limitations of current sensors on time and spatial resolution, the role of many extracellular analytes is still unknown. A single walled carbon nanotube (SWNT) platform was previously developed based on the avidin-biotin immobilization of SWNT to a glass substrate. The SWNT platform provides real time feedback about analyte concentration and has a high concentration of evenly distributed sensors, both of which are essential for the study of extracellular analytes. Unfortunately, this initial SWNT platform is synthesized through unsterile conditions and cannot be sterilized post-production due to the delicate nature of the sensors, making it unsuitable for in vitro work. Herein the multiple-step process for SWNT immobilization is modified and the platform's biocompatibility is assessed in terms of sterility, cytotoxicity, cell proliferation, and cell morphology through comparison with non-sensors controls. The results demonstrate the SWNT platform's sterility and lack of toxicity over 72 h. The proliferation rate and morphology profiles for cells growing on the SWNT platform are similar to those grown on tissue culture substrates. This novel nano-sensor platform preserves cell health and cell functionality over time, offering opportunities to study extracellular analytes gradients in cellular communication.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0