Analytical biochemistry最新文献_第9页

Disulfide-adducts with cysteine residues in human serum albumin prove exposure to malodorous mercaptans in vitro 人血清白蛋白中半胱氨酸残基的二硫化物加合物证明体外接触了恶臭硫醇。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-13 DOI: 10.1016/j.ab.2024.115568

Paula Helena Sieber , Dirk Steinritz , Franz Worek , Harald John

{"title":"Disulfide-adducts with cysteine residues in human serum albumin prove exposure to malodorous mercaptans in vitro","authors":"Paula Helena Sieber , Dirk Steinritz , Franz Worek , Harald John","doi":"10.1016/j.ab.2024.115568","DOIUrl":"10.1016/j.ab.2024.115568","url":null,"abstract":"<div>Malodorants are mixtures containing mercaptans, which trigger the flight instinct upon exposure and might thus be deployed in military and civilian defense scenarios. Exposure to mercaptans might lead to unconsciousness, thus representing a possible threat for health. Therefore, we developed and validated a bioanalytical procedure for the simultaneous detection and identification of corresponding biomarkers for the verification of exposure to mercaptans. Disulfide-adducts of ethyl mercaptan (SEt), n-butyl mercaptan (SnBu), tert-butyl mercaptan (StBu) and iso-amyl mercaptan (SiAm) with cysteine (Cys) residues in human serum albumin (HSA) were formed by in vitro incubation of human plasma. After pronase-catalyzed proteolysis, reaction products were identified as adducts of the single amino acid Cys and the dipeptide cysteine-proline (Cys34Pro) detected by a sensitive μLC-ESI MS/MS method working in the scheduled multiple reaction monitoring (sMRM) mode. Dose-response studies showed linearity for the yield of Cys34Pro-adducts in the range from 6 nM to 300 μM of mercaptans in plasma and limits of identification (LOI) were in the range from 60 nM to 6 μM. Cys34-adducts showed stability for at least 6 days in plasma (37 °C). The presented disulfide-biomarkers expand the spectrum for bioanalytical verification procedures and might be helpful to prove exposure to malodorants.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S000326972400112X/pdfft?md5=65df707b16fe72505363b665d413aee9&pid=1-s2.0-S000326972400112X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140943813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A reproducible and affordable method of conducting luciferase assay 一种可重复且经济实惠的荧光素酶检测方法。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-10 DOI: 10.1016/j.ab.2024.115558

Kirtana Reddy, Balaji Ramachandran

引用次数: 0

ICP-MS based detection method combined with Au NP and Ag NP labeling for bacteremia diagnosis 基于 ICP-MS 的检测方法与 Au NP 和 Ag NP 标记相结合用于菌血症诊断。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-08 DOI: 10.1016/j.ab.2024.115559

Xiaoting Li , Xin Li , Jiayi Chen , Zuomin Tan , Chuan Wang

{"title":"ICP-MS based detection method combined with Au NP and Ag NP labeling for bacteremia diagnosis","authors":"Xiaoting Li , Xin Li , Jiayi Chen , Zuomin Tan , Chuan Wang","doi":"10.1016/j.ab.2024.115559","DOIUrl":"10.1016/j.ab.2024.115559","url":null,"abstract":"<div>Bacteremia, as a serious infectious disease, has an increasing incidence and a high mortality rate. Early diagnosis and early treatment are crucial for improving the cure rate. In this work, we proposed an inductively coupled plasma mass spectrometry (ICP-MS)-based detection method combined with gold nanoparticle (Au NP) and silver nanoparticle (Ag NP) labeling for the simultaneous detection of Salmonella and Escherichia coli (E. coli O157:H7) in human blood samples. Salmonella and E. coli O157:H7 were captured by magnetic beads coupled with anti-8G3 and anti-7C2, and then specifically labeled by Au NP-anti-5H12 and Ag NP-anti-8B1 respectively, which were used as signal probes for ICP-MS detection. Under the optimal experimental conditions, the limits of detection of 164 CFU mL−1 for Salmonella, 220 CFU mL−1for E. coli O157:H7 and the linear ranges of 400–80,000 CFU mL−1 Salmonella, 400–60,000 CFU mL−1 E. coli O157:H7 were obtained. The proposed method can realize the simultaneous detection of two types of pathogenic bacteria in human whole blood in 3.5 h, showing great potential for the rapid diagnosis of bacteremia in clinic.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome c electrochemical detection utilizing molecularly imprinted poly(3, 4-ethylenedioxythiophene) on a disposable screen printed carbon electrode 利用一次性丝网印刷碳电极上的分子印迹聚（3, 4-亚乙二氧基噻吩）进行细胞色素 c 电化学检测。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-07 DOI: 10.1016/j.ab.2024.115557

Ganesan Kaniraja , Murugesan Karthikeyan , Marimuthu Dhinesh Kumar , Periyasamy Ananthappan , Karuppiah Arunsunai Kumar , Vellasamy Shanmugaiah , Vairathevar Sivasamy Vasantha , Chandran Karunakaran

{"title":"Cytochrome c electrochemical detection utilizing molecularly imprinted poly(3, 4-ethylenedioxythiophene) on a disposable screen printed carbon electrode","authors":"Ganesan Kaniraja , Murugesan Karthikeyan , Marimuthu Dhinesh Kumar , Periyasamy Ananthappan , Karuppiah Arunsunai Kumar , Vellasamy Shanmugaiah , Vairathevar Sivasamy Vasantha , Chandran Karunakaran","doi":"10.1016/j.ab.2024.115557","DOIUrl":"10.1016/j.ab.2024.115557","url":null,"abstract":"<div>Cytochrome c (cyt c) has been found to play a function in apoptosis in cell-free models. This work presents the creation of molecularly imprinted conducting poly(3, 4-ethylenedioxythiopene) (MIPEDOT) on the surface of a screen printed carbon electrode (SPCE) for cyt c. Cyt c was imprinted by electropolymerization due to the presence of an EDOT monomer hydrophobic functional group on SPCE, using CV to obtain highly selective materials with excellent molecular recognition ability. MIPEDOT was characterized by CV, EIS, and DPV using ferricyanide/ferrocyanide as a redox probe. Further, the characterization of the sensor was accomplished using SEM for surface morphological confirmation. Using CV, the peak current measured at the potential of +1 to −1 V (vs. Ag/AgCl) is linear in the cyt c concentration range from 1 to 1200 pM, showing a remarkably low detection limit of 0.5 pM (sensitivity:0.080 μA pM). Moreover, the applicability of the approach was successfully confirmed with the detection of cyt c in biological samples (human plasma). Similarly, our research has proven a low-cost, simple, and efficient sensing platform for cyt c detection, rendering it a viable tool for the future improvement of reliable and exact non-encroaching cell death detection.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rhodamine-based fluorescent probe used to determine nitroxyl (HNO) in lysosomes 一种基于罗丹明的荧光探针，用于测定溶酶体中的硝基（HNO）。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-06 DOI: 10.1016/j.ab.2024.115552

Shuangyu Liu , Junhong Xu , Qiujuan Ma , Linke Li , Guojiang Mao , Gege Wang , Xiaowei Wu

{"title":"A rhodamine-based fluorescent probe used to determine nitroxyl (HNO) in lysosomes","authors":"Shuangyu Liu , Junhong Xu , Qiujuan Ma , Linke Li , Guojiang Mao , Gege Wang , Xiaowei Wu","doi":"10.1016/j.ab.2024.115552","DOIUrl":"10.1016/j.ab.2024.115552","url":null,"abstract":"<div>The reactive nitrogen species (RNS) in lysosomes play a major role during the regulation of lysosomal microenvironment. Nitroxyl (HNO) belongs to active nitrogen species (RNS) and is becoming a potential diagnostic and therapeutic biomarker. However, the complex synthesis routes of HNO in biosystem always hinder the exact determination of HNO in living cells. Here, a rhodamine-based fluorescent probe used to determine nitroxyl (HNO) in lysosomes was constructed and synthesized. 2-(Diphenylphosphino)benzoate was utilized as the sensing unit for HNO and morpholine was chose as the targeting group for lysosome. Before the addition of HNO, the probe displayed a spirolactone structure and almost no fluorescence was found. After the addition of HNO, the probe existed as a conjugated xanthene form and an intense green fluorescence was observed. The fluorescent probe possessed fast response (3 min) and high selectivity for HNO. Furthermore, fluorescence intensity of the probe linearly related with the HNO concentration in the range of 6.0 × 10−8 to 6.0 × 10−5 mol L−1. The detection limit was found to be 1.87 × 10−8 mol L−1 for HNO. Moreover, the probe could selectively targeted lysosome with excellent biocompatibility and had been effectually utilized to recognize exogenous HNO in A549 cells.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting associations between CircRNA and diseases through structure-aware graph transformer and path-integral convolution 通过结构感知图变换器和路径积分卷积预测 CircRNA 与疾病的关联性

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-04 DOI: 10.1016/j.ab.2024.115554

Jinkai Wu, PengLi Lu, Wenqi Zhang

{"title":"Predicting associations between CircRNA and diseases through structure-aware graph transformer and path-integral convolution","authors":"Jinkai Wu, PengLi Lu, Wenqi Zhang","doi":"10.1016/j.ab.2024.115554","DOIUrl":"10.1016/j.ab.2024.115554","url":null,"abstract":"<div>A series of biological experiments has demonstrated that circular RNAs play a crucial regulatory role in cellular processes and may be potentially associated with diseases. Uncovering these connections helps in understanding potential disease mechanisms and advancing the development of treatment strategies. However, in biology, traditional experiments face limitations in terms of efficiency and cost, especially when enumerating possible associations. To address these limitations, several computational methods have been proposed, but existing methods only measure from a nodal perspective and cannot capture structural similarities between edges. In this study, we introduce an advanced computational method called SATPIC2CD for analyzing potential associations between circular RNAs and diseases. Specifically, we first employ an Structure-Aware Graph Transformer (SAT), which extracts five predefined metapath representations before calculating attention. This adaptive network integrates structural information into the original self-attention by aggregating information within and between paths. Subsequently, we use Path Integral Convolutional Networks (PACN) to integrate feature information for all path weights between two nodes. Afterward, we complement the network node features with feature loss and feature smoothing using Gated Recurrent Units (GRU) and node centrality. Finally, a Multi-Layer Perceptron (MLP) is employed to obtain the ultimate prediction scores for each circular RNA-disease pair. SATPIC2CD performs remarkably well, with an accuracy of up to 0.9715 measured by the Area Under the Curve (AUC) in a 5-fold cross-validation, surpassing other comparative models. Case studies further emphasize the high precision of our method in identifying circular RNA-disease associations, laying a solid foundation for guiding future biological research efforts.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive and effective method with 96-well plate for determination of levamlodipine in human plasma using LC–MS/MS 利用液相色谱-串联质谱/质谱（LC-MS/MS）技术，采用 96 孔板灵敏有效地测定人血浆中的左旋氨氯地平

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-03 DOI: 10.1016/j.ab.2024.115556

Lingfang Dong , Kun Li , Shan Gao , Wenyi Wang , Yinghua Feng , Jingying Zhang , Jie Yang , Weifeng Dong , Wei Zhang

{"title":"Sensitive and effective method with 96-well plate for determination of levamlodipine in human plasma using LC–MS/MS","authors":"Lingfang Dong , Kun Li , Shan Gao , Wenyi Wang , Yinghua Feng , Jingying Zhang , Jie Yang , Weifeng Dong , Wei Zhang","doi":"10.1016/j.ab.2024.115556","DOIUrl":"https://doi.org/10.1016/j.ab.2024.115556","url":null,"abstract":"<div>we developed an effective protein precipitation method for determination of levamlodipine in human plasma using LC–MS/MS. Sample extraction was carried out by using liquid–liquid extraction in 96-well plate format. (S)-Amlodipine-d4 was used as internal standard (IS). The chromatographic separation was achieved using Philomen Chiral MX (2) column (3 μm, 2.1 × 100 mm). Mobile phase A was comprised of Acetonitrile (ACN), Mono ethanol amine (MEA) and Iso-Propyl alcohol (IPA) (1000:1:10, v/v/v), Mobile phase B was IPA-ACN (2:1, v/v). The flow rate was 0.4 mL/min. The total run time of each sample was 4.0 min with gradient elution. LC-MS/MS spectra were generated in positive ion mode, and multiple reaction monitoring (MRM) was used to detect the following transitions: m/z 409.20 → 238.15 for levamlodipine and 415.25 → 240.20 for (S)-Amlodipine-d4 (the IS). The method was linear from 50 to 10000 pg/mL（R2＝0.9988489）,and the lower limit of quantification (LLOQ) was 50 pg/mL. This method was applied to a bioequivalence study of levamlodipine.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140844346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing nitric acid treatment and microwave digestion for efficiency of metal extraction from bioprocess samples 比较硝酸处理和微波消解从生物处理样品中提取金属的效率。

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-03 DOI: 10.1016/j.ab.2024.115555

Adil Mohammad, Chikkathur N. Madhavarao

{"title":"Comparing nitric acid treatment and microwave digestion for efficiency of metal extraction from bioprocess samples","authors":"Adil Mohammad, Chikkathur N. Madhavarao","doi":"10.1016/j.ab.2024.115555","DOIUrl":"10.1016/j.ab.2024.115555","url":null,"abstract":"<div>Metal ions may act as enzyme cofactors and influence the kinetics of biochemical reactions that may also influence the biological production of therapeutic proteins and quality attributes such as glycosylation. Because sample preparation is a significant step in the reliable analysis of metals, we compared two sample preparation procedures for metal analysis of bioreactor culture media samples by ICP-MS: (i) samples were diluted in 2 % nitric acid (treatment with nitric acid, TNA); and (ii) samples were mixed with equal volume of 5 % nitric acid and closed vessel digestion was performed in a microwave (closed vessel digestion, CVD). In the comparison of extraction efficiencies between TNA and CVD procedures, CVD showed better extraction for Ca and Cu among bulk metals (∼30 %) and for Ni among the trace metals (∼65 %) for the bioreactor broth supernatant samples. For the cell pellet samples, the CVD procedure was found to be better for extraction of Fe (∼65 % more) among bulk metals, Zn (∼20 % more) among minor metals and Co (∼60 % more) and Ni (∼45 % more) among trace metals. Differences between the two procedures were less than 10 % and TNA was better for all other metals quantified from both supernatant samples and cell pellet samples. The current study helps bring more clarity to the methodology on comprehensive metal analysis to monitor and maintain trace metal content for biologics production.</div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical sensor based on molecularly imprinted poly(o-phenylenediamine) for the detection of thymol 基于分子印迹聚（邻苯二胺）的电化学传感器用于检测百里酚

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-01 DOI: 10.1016/j.ab.2024.115551

Jieming Dong , Hongyu Zhang , Zhixiang Ding , Junyao Li , Laidi Xu , Yong Kong , Guojun Zheng

引用次数: 0

Microwave-assisted and methanol/acetic acid-free method for rapid staining of proteins in SDS-PAGE gels 微波辅助且不含甲醇/醋酸的 SDS-PAGE 凝胶蛋白质快速染色法

IF 2.9 4区生物学

Analytical biochemistry Pub Date : 2024-05-01 DOI: 10.1016/j.ab.2024.115553

Jinzhong Wang , Zhaoyang Wang , Wei Zhao , Ying Wang

引用次数: 0