Analytical biochemistry最新文献

{"title":"Evaluation of active substances in gamboge and their mechanisms for the treatment of colorectal cancer by UPLC-MS/MS integrated with network pharmacology","authors":"Guodong Shan ,&nbsp;Jiajun Jiang ,&nbsp;Liting Ji ,&nbsp;Shiyan Li ,&nbsp;Zejun Wang ,&nbsp;Shaohui Yang ,&nbsp;Qing Shen","doi":"10.1016/j.ab.2024.115747","DOIUrl":"10.1016/j.ab.2024.115747","url":null,"abstract":"<div><div>Gamboge exhibits anti-colorectal cancer (CRC) activity, however, its active compounds and the underlying mechanisms remain unclear. Herein, a liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for determining gambogellic acid, β-morellic acid, isogambogenic acid, gambogenic acid, R-gambogic acid, S-gambogic acid, and hydroxygambogic acid in gamboge was established. The key parameters including ion transitions, voltages, LOD, and LOQ were determined, with LOD ranging from 0.8 to 2.0 ng mL⁻¹ and LOQ from 2.7 to 6.7 ng mL⁻¹. The recovery rates were found to be between 95.6 % and 103.5 %. Furthermore, the active compounds were successfully determined, and molecular mechanisms of gamboge in treating CRC were explored. Network pharmacology revealed a “compound-target-pathway” network where the seven compounds could target key proteins, modulate PI3K-Akt and JAK-STAT pathways, and inhibit CRC development. Molecular docking validated SRC, SATA3, PIK3CA, among others, as potential targets for the active compounds in CRC intervention. In conclusion, this method significantly reduces analysis time and improves efficiency relative to existing approaches, making it highly suitable for the effective determination of multiple compounds in the quality control of gamboge materials.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115747"},"PeriodicalIF":2.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of different biotechnologies in detecting the changes in MAM and their classic discoveries","authors":"Shuangshuang Zhang ,&nbsp;Yingchun Shao ,&nbsp;Mengzhu Su ,&nbsp;Yuerong Hao ,&nbsp;Yang Yuan ,&nbsp;Dongming Xing","doi":"10.1016/j.ab.2024.115744","DOIUrl":"10.1016/j.ab.2024.115744","url":null,"abstract":"<div><div>Mitochondria-associated membrane (MAM) has been studied as a novel target for explaining the mechanisms underlying the changes in cellular function and the process of multiple diseases. This structure is a complex of proteins, it tethers mitochondria to the endoplasmic/sarcoplasmic reticulum (ER/SR) and mediates the crosstalk of ions, lipids and metabolites between the two organelles. Different component proteins play distinctive ways in influencing the structure of MAM or the cellular signal transduction. Mitochondria and ER are the hubs of cellular bioenergetics and protein homeostasis respectively, MAM was supposed to play both physiological and pathological roles in regulating the function of either the two organelles and cells. The mitochondria-associated membrane is a highly dynamic structure and could be disrupted or remodelled within several minutes. Up to now, not all component proteins of the MAM complex have been revealed. Several biochemical and imaging approaches have been used to measure the changes in the structure or the number of MAMs, but they come with their issues. For distinct research aims, particular techniques were used based on their applicabilities, the research platforms and technical defects. This review briefly summarized the current biotechnologies for detecting MAM, and analyzed their limitations, aiming to assist further research in selecting appropriate methods based on actual situations.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115744"},"PeriodicalIF":2.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreserved PBMCs can be used for the analysis of mitochondrial respiration and serve as a diagnostic tool for mitochondrial diseases","authors":"Zuzana Korandová ,&nbsp;Petr Pecina ,&nbsp;Alena Pecinová ,&nbsp;Eliška Koňaříková ,&nbsp;Markéta Tesařová ,&nbsp;Josef Houštěk ,&nbsp;Hana Hansíková ,&nbsp;Hana Ptáčková ,&nbsp;Jiří Zeman ,&nbsp;Tomáš Honzík ,&nbsp;Tomáš Mráček","doi":"10.1016/j.ab.2024.115745","DOIUrl":"10.1016/j.ab.2024.115745","url":null,"abstract":"<div><div>Mitochondrial diseases are severe, inherited metabolic disorders that affect the paediatric population. They affect the functioning of mitochondrial oxidative phosphorylation (OXPHOS) apparatus either directly or indirectly. Since mutations in mtDNA are responsible for only 25 % of paediatric cases and next-generation sequencing does not always provide a conclusive diagnosis, the biochemical approach still represents a valuable tool in diagnostics. Mitochondrial defects can be identified in tissue biopsies (muscle or skin). However, they also often manifest in peripheral blood cells. We developed a protocol for isolation and cryopreservation of peripheral blood mononuclear cells (PBMCs) from 5 ml of children's blood using Ficoll centrifugation which can be utilised for subsequent functional measurements on thawed samples. Furthermore, we evaluated the diagnostic utility of the optimised high-resolution oxygraphy protocol using digitonin-permeabilized cryopreserved PBMCs on 47 samples from patients with confirmed or suspected mitochondrial disease. Overall, the diagnosis was confirmed in 72 % of cases, while the analysis of cryopreserved PBMCs provided a false negative outcome in 13 % of cases. Our study demonstrates a sensitive, fast, and non-invasive approach for the diagnostics of various types of mitochondrial disorders, especially those of nuclear genetic origin manifesting in paediatric patients.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115745"},"PeriodicalIF":2.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical determination of ascorbic acid using sensitive and disposable methylene blue modified pencil graphite electrode","authors":"K.G. Aishwarya,&nbsp;Y. Arthoba Nayaka,&nbsp;E. Pradeepa,&nbsp;H.R. Sahana","doi":"10.1016/j.ab.2024.115733","DOIUrl":"10.1016/j.ab.2024.115733","url":null,"abstract":"<div><div>In the present work, a convenient, efficient and disposable electrochemical sensor has been developed by electropolymerizing methylene blue (PMB) on the surface of a pencil graphite electrode (PGE), which facilitates the electrochemical analysis of an antioxidant <span>l</span>-Ascorbic Acid (AA). The structural characteristics of both the methylene blue modified pencil graphite electrode (PMB/PGE) and the bare pencil graphite electrode (BPGE) have been examined using scanning electron microscopy (SEM) in conjunction with energy-dispersive X-ray analysis (EDX). Additionally, the charge transfer behavior has been evaluated using the electron impedance spectroscopy (EIS). The voltammetric response of AA has been examined using different methods, such as differential pulse voltammetry (DPV) and linear sweep voltammetry (LSV). This exploration has been carried out in 0.1 M phosphate buffer solution (PBS) of physiological pH 7.0. The electrochemical sensor PMB/PGE proposed in this study exhibited an improved peak current and a slight negative shift in peak potential for AA compared to bare electrode. The enhancement in peak current at the modified electrode has been attributed to the electrocatalytic characteristics of the modifiers. The limit of detection (LOD) for AA has been determined using the differential pulse voltammetry (DPV), with concentrations ranging from 1.0 μM to 12.0 μM. The calculated LOD value has been found to be 0.15 μM. The selectivity and practicality of the modified electrode has been assessed through the real sample analysis and demonstrating its capability to detect AA in the presence of paracetamol (PA) resulting in satisfactory recovery results. Hence the proposed sensor could be successfully validated for the determination of AA in pharmaceutical sample.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115733"},"PeriodicalIF":2.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a sensitive and fast bioanalytical LC-MS/MS assay for the quantitation of venetoclax and azacitidine in Rat Plasma: Application to pharmacokinetic study","authors":"Manal El-Gendy ,&nbsp;Mohamed Hefnawy ,&nbsp;Sarah Alrubia ,&nbsp;Abdulaziz Alnasser ,&nbsp;Amsha Alsegiani ,&nbsp;Yousef Bin Jardan ,&nbsp;Adel El-Azab ,&nbsp;Alaa Abdel-Aziz ,&nbsp;Mohamed Attwa ,&nbsp;Emad Alsarhani","doi":"10.1016/j.ab.2024.115741","DOIUrl":"10.1016/j.ab.2024.115741","url":null,"abstract":"<div><div>The combination of venetoclax plus azacitidine (VTX-AZA) is FDA-approved to treat patients with acute myeloid leukemia (AML) aged ≥75 years and has become the standard of care for AML patients. However, the literature has not reported an analytical method for determining VTX-AZA in plasma samples. Therefore, developing an accurate and sensitive bioanalytical assay to quantify VTX-AZA in plasma is important. For the first time, this study describes the development of a new liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of VTX and AZC in plasma samples with its application to pharmacokinetic study in rats. The assay employs repaglinide (RPG) as an internal standard. The chromatographic separations of VTX, AZC, and RPG are achieved within 2.5 min at 25 °C on an Eclipse plus C18 column (100 mm × 2.1 mm, 1.8 μm) and an isocratic mobile phase consisted of water with 0.1 % formic acid and acetonitrile (50:50, v/v, pH 3.2) at a flow rate of 0.30 mL/min. VTX and AZC have been extracted from rat plasma using the solid-phase extraction (SPE) procedure without interference from plasma endogenous. The FDA guidelines were followed in the validation of the developed assay, and linearity in rat plasma was observed for AZC and VTX, respectively, ranging from 5 to 3000 and 5–1000 ng/mL, with r ≥ 0.998. The lower limits of detection (LLOD) were 2 ng/mL for both drugs. In addition, the inter-day and intra-day accuracy were 0.8–6.6 % and 2.2–5.7 %; the inter-day and intra-day precision were 3–6.6 % and 1.5–7.1 %, respectively. The validated assay was effectively used in a pharmacokinetic investigation including the simultaneous oral administration of 40 mg/kg of AZA and 100 mg/kg of VTX to rats. The maximum plasma concentration (C_max) for AZC and VTX was 794 ± 99.6 ng/mL and 641 ± 96.9 ng/mL achieved at 0.5 ± 0.03 h and 6 ± 0.05 h, respectively. The AUC_0-∞ for AZC and VTX was 1253 ± 252.6 and 4881 ± 745.4 ng/mL.h; respectively.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115741"},"PeriodicalIF":2.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-resolution targeted mass spectrometry for comprehensive quantification of sphingolipids: clinical applications and characterization of extracellular vesicles","authors":"Thiago V.D. Felippe ,&nbsp;Diana M. Toro ,&nbsp;Jonatan C.S. de Carvalho ,&nbsp;Pedro Nobre-Azevedo ,&nbsp;Luiz F.M. Rodrigues ,&nbsp;Bianca T.M. Oliveira ,&nbsp;Pedro V. da Silva-Neto ,&nbsp;Adriana F.L. Vilela ,&nbsp;Fausto Almeida ,&nbsp;Lúcia H. Faccioli ,&nbsp;Carlos A. Sorgi","doi":"10.1016/j.ab.2024.115732","DOIUrl":"10.1016/j.ab.2024.115732","url":null,"abstract":"<div><div>Sphingolipids (SL), a class of membrane lipids, play important roles in numerous biological processes. Their significant structural diversity poses challenges for accurate quantification. To address this, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for sphingolipidomics, capable of profiling these lipids comprehensively. In this study, we utilized LC-MS/MS with high-resolution mass spectrometry (MRM^HR) to develop a targeted method for the identification and quantification of various SL species. This method, based on validated parameters such as precursor/fragment ions (<em>m/z</em>) and retention time, demonstrated high sensitivity and accuracy, successfully identifying SL species across 12 distinct classes. Its open-panel design also facilitates the analysis of new SL-species targets. Notably, using this approach, we identified 40 SL species in plasma samples from COVID-19 patients, and we determined the influence of matrix metalloproteinase-3 (MMP-3) expression on the positive downstream of SL metabolism. Beyond plasma analysis, this method has potential applications in other biomedical contexts, such as extracellular vesicles (EVs), describing the cargo of sphingosine-1-phosphate (S1P) on macrophage-derived EVs. The establishment of this targeted workflow enabling precise quantification of a wide range of SL species, holds promise for identifying novel biomarkers and therapeutic targets.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115732"},"PeriodicalIF":2.6,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive performance of a centrosome-associated prognostic model in prognosis and immunotherapy of lung adenocarcinoma","authors":"Feng Yan ,&nbsp;Qian Guo ,&nbsp;Rongbing Zheng ,&nbsp;Jiongming Ying","doi":"10.1016/j.ab.2024.115731","DOIUrl":"10.1016/j.ab.2024.115731","url":null,"abstract":"<div><div>In recent years, mounting investigations have highlighted the pivotal role of centrosomes in cancer progression. In this study, we employed bioinformatics and statistics to establish a 13-centrosome-associated gene prognostic model for lung adenocarcinoma (LUAD) utilizing transcriptomic data from TCGA. Based on the Riskscore, patients were stratified into high- and low-risk groups. Through survival analysis and receiver operating characteristic curve analysis, our model demonstrated a consistent and robust prognostic capacity, which was further validated using the GEO database. Univariate/multivariate Cox regression analyses identified our model as an independent prognostic factor for LUAD patients. Subsequently, immunoinfiltration analysis showed that immune cell infiltration levels of aDCs, iDCs, Mast cells, and Neutrophils, as well as immune functionalities such as HLA, Type I IFN Response and Type II IFN Response, were markedly reduced in the high-risk group compared to the low-risk group. Finally, we conducted a drug screening to identify potential treatments for patients with different prognoses. We utilized the GDSC database and molecular docking techniques to identify small molecule compounds targeting the prognostic genes. In conclusion, our prognostic model exhibits robust and reliable predictive capability, and it may have important clinical implications in guiding treatment decisions for LUAD patients.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115731"},"PeriodicalIF":2.6,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A molecularly imprinted electrochemical sensor based on in-situ polymerization for rapid and selective detection of tonalide in aqueous environment","authors":"Chengxin Su ,&nbsp;Xiaoling Liu ,&nbsp;Ke Zhang ,&nbsp;Bing Jiang ,&nbsp;Jiashuai Hu ,&nbsp;Mei Li ,&nbsp;Lin Cheng ,&nbsp;Hongbing Luo ,&nbsp;Wanchen Xie ,&nbsp;Cheng Liu ,&nbsp;Liangqian Fan ,&nbsp;Wei Chen ,&nbsp;Xiaohong Zhang","doi":"10.1016/j.ab.2024.115730","DOIUrl":"10.1016/j.ab.2024.115730","url":null,"abstract":"<div><div>Given the adverse effects of tonalide (AHTN) on aquatic organisms and humans, coupled with the limitations of current detection methods, which are time-consuming, require expensive equipment and complicated sample preparation procedures, there is a clear need to develop a new technique for detecting AHTN that is highly sensitive, rapid, cost-effective and efficient. In this study, a new simple electrochemical sensor for the determination of AHTN in aqueous environments was developed for the first time through the in-situ polymerization of an AHTN-imprinted polymer on the surface of a graphene (G)-modified carbon electrode (GCE). Following a series of comparative tests, including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), the novel AHTN molecularly imprinted sensor (AHTN-MIP/G/GCE) has been demonstrated to be an effective tool for monitoring AHTN. The results demonstrate that the linear detection range of the current response of the AHTN-MIP/G/GCE ¹electrode to AHTN was 0.01 μM–4 μM (i.e., 2.584 μg/L-1033.6 μg/L), with a detection limit of 2.3 × 10⁻⁹ M (i.e., 594.32 ng/L), following the optimization of the experimental conditions. Furthermore, the new sensor was successfully employed for the detection of AHTN in water samples, with recoveries of 97.1%–108.2 % with the added standards. Consequently, the new electrochemical sensor demonstrated good stability and acceptable reproducibility. This study provides a new method for the future detection of AHTN in the aqueous environment.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115730"},"PeriodicalIF":2.6,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of chemical unfolding complexity as a unique stability assessment assay for monoclonal antibodies","authors":"J. Alaina Floyd ,&nbsp;Jeremy M. Shaver","doi":"10.1016/j.ab.2024.115729","DOIUrl":"10.1016/j.ab.2024.115729","url":null,"abstract":"<div><div>Seventy-two intentionally sequence-diverse antibody variable regions were selected, expressed as IgG1 antibodies, and evaluated by chemical unfolding to survey the complexities of denaturant induced unfolding behavior. A two-transition fit well described the curves and uncovered a wide range of sensitivities to denaturant. Four general types of unfolding curves were observed: balanced traces (each transition responsible for half of the total unfolding curve), low-unfolding traces (first transition is a majority of the unfolding curve), high-unfolding traces (the second transition is the majority of the unfolding curve), and coincident traces (the two transitions are found close to each other, approximating a single transition). The complexity of the data from this survey indicates that focusing on the first inflection point or fitting a single transition model is likely an over-simplistic method for measuring stability by the chemical unfolding assay. Additionally, other conformational assays, such as thermal and low pH unfolding, showed no correlation with the chemical unfolding results, indicating that each of these assays provide alternate information on the different pathways of antibody conformational stability. These results provide a basis for beginning to better connect unfolding behavior to other physical phenotypic behaviors and production process behaviors.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115729"},"PeriodicalIF":2.6,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical sensing of dopamine using nanostructured silver chromate: Development of an IoT-integrated sensor","authors":"Lungelo Mgenge ,&nbsp;Chandan Saha ,&nbsp;Pooja Kumari ,&nbsp;Sarit K. Ghosh ,&nbsp;Harishchandra Singh ,&nbsp;Kaushik Mallick","doi":"10.1016/j.ab.2024.115726","DOIUrl":"10.1016/j.ab.2024.115726","url":null,"abstract":"<div><div>Dopamine, one of the most important neurotransmitters, plays a crucial role in the functions of human metabolism, as well as the cardiovascular, central nervous and hormonal systems. This study focuses on the synthesis of nanostructured silver chromate and their application in dopamine sensing. The nanoparticles were synthesized using a complexation-mediated route using aminosalicylic acid as a stabilizer, resulting in uniform particles ranging from 3 to 15 nm in size. The synthesized silver chromate was characterized using X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy techniques. Electrochemical studies revealed that the silver chromate exhibit excellent catalytic activity for the detection of dopamine. The electroanalysis provided the selective recognition of dopamine with the limit of detection of 1.05 μM and sensitivity of 2.68 μA μM⁻¹ cm⁻² in a linear range of 5–45 μM. Additionally, a portable, IoT (internet of things)-integrated sensor based on the synthesized silver chromate was developed using Arduino Uno R4 Wi-Fi module, enabling real-time monitoring of dopamine with data transmission to a cloud platform.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115726"},"PeriodicalIF":2.6,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}