Analytical biochemistry最新文献_第8页

Differentiation of glioblastoma G4 and two types of meningiomas using FTIR spectra and machine learning 利用FTIR光谱和机器学习鉴别胶质母细胞瘤G4和两种脑膜瘤。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-27 DOI: 10.1016/j.ab.2024.115754

Bartłomiej Tołpa , Wiesław Paja , Paweł Jakubczyk , Kornelia Łach , Elżbieta Trojnar , Agnieszka Gala-Błądzińska , Aneta Kowal , Bartosz Klębowski , Jozef Cebulski , Joanna Depciuch

{"title":"Differentiation of glioblastoma G4 and two types of meningiomas using FTIR spectra and machine learning","authors":"Bartłomiej Tołpa , Wiesław Paja , Paweł Jakubczyk , Kornelia Łach , Elżbieta Trojnar , Agnieszka Gala-Błądzińska , Aneta Kowal , Bartosz Klębowski , Jozef Cebulski , Joanna Depciuch","doi":"10.1016/j.ab.2024.115754","DOIUrl":"10.1016/j.ab.2024.115754","url":null,"abstract":"<div><div>Brain tumors are among the most dangerous, due to their location in the organ that governs all life processes. Moreover, the high differentiation of these poses a challenge in diagnostics. Therefore, this study focused on the chemical differentiation of glioblastoma G4 (GBM) and two types of meningiomas (atypical - MAtyp and angiomatous - MAng) were done using Fourier Transform InfraRed (FTIR) spectroscopy, combined with statistical, multivariate, machine learning and rate of spectrum changes methods. The positions of all analyzed peaks differed between GBM and meningiomas. However, for two types of meningiomas, only shift of peaks corresponding to CH<sub>2</sub> bending vibrations, symmetric stretching vibrations of CH<sub>2,</sub> amide A, amide I, C<img>O lipids vibrations, asymmetric stretching vibrations of CH<sub>3</sub> were observed. Principal Component Analysis showed clear differentiation between GBM and the meningiomas. Decision tree clearly showed that wavenumbers corresponding to C<img>O lipids vibrations provided the highest differentiation between GBM and meningiomas tissues, while amide I for two types of meningiomas. The accuracy and specificity of the results for GBM and meningiomas were more than 90 %, while for MAtyp and MAng, these parameters were around 80 %.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"699 ","pages":"Article 115754"},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent focus in non-SELEX-computational approach for de novo aptamer design: A mini review 最近的焦点在非选择性计算方法的从头适体设计：一个小回顾。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-26 DOI: 10.1016/j.ab.2024.115756

Ilemobayo Victor Fasogbon , Erick Nyakundi Ondari , Deusdedit Tusubira , Loganathan Rangasamy , Janarthanan Venkatesan , Angela Mumbua Musyoka , Patrick Maduabuchi Aja

{"title":"Recent focus in non-SELEX-computational approach for de novo aptamer design: A mini review","authors":"Ilemobayo Victor Fasogbon , Erick Nyakundi Ondari , Deusdedit Tusubira , Loganathan Rangasamy , Janarthanan Venkatesan , Angela Mumbua Musyoka , Patrick Maduabuchi Aja","doi":"10.1016/j.ab.2024.115756","DOIUrl":"10.1016/j.ab.2024.115756","url":null,"abstract":"<div><div>Aptamers, single-stranded nucleic acids that bind to specific targets with high affinity and specificity, hold significant promise in various biomedical and biotechnological applications.</div><div>The traditional method of aptamer selection, SELEX (Systematic Evolution of Ligands by EXponential Enrichment) takes a lot of work and time. Recent advancements in computational methods have revolutionized aptamer design, offering efficient and effective alternatives. This review examines recent advances in non-SELEX and de novo aptamer design methods, such as Making Aptamers without SELEX (MAWS), AptaLoop, AptaDiff, RNAGEN, RaptGen, Apta-MCTS, UltraSelex, and Torkamanian-Afshar model. These computer methods utilize bioinformatics, machine learning, and molecular modeling to generate high-affinity aptamers, eliminating the need for multiple selection steps <em>in vitro</em> or <em>in vivo</em>. We provide a comprehensive analysis of each method's performance, including binding affinity, specificity, and stability, and discuss their practical applications in diagnostics, therapeutics, and environmental monitoring. Furthermore, we highlight the strengths and limitations of computational methods against the traditional one. The potential challenges, future directions, and emerging.technologies were also presented. This review underscores the transformative impact of computational aptamer design on research and industry, paving the way for rapid and cost-effective development of aptamer-based technologies.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"699 ","pages":"Article 115756"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the association between serum magnesium level and autism spectrum disorder using validated spectrofluorimetric method 用有效的荧光光谱法探讨血清镁水平与自闭症谱系障碍的关系。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-26 DOI: 10.1016/j.ab.2024.115755

Atiah H. Almalki , Ahmed K. Bamaga , Adnan Alharbi , Maram H. Abduljabbar , Reem M. Alnemari , Fahad H. Baali , Majed A. Algarni , Marwa F. Ahmed , Sherif Ramzy

{"title":"Exploring the association between serum magnesium level and autism spectrum disorder using validated spectrofluorimetric method","authors":"Atiah H. Almalki , Ahmed K. Bamaga , Adnan Alharbi , Maram H. Abduljabbar , Reem M. Alnemari , Fahad H. Baali , Majed A. Algarni , Marwa F. Ahmed , Sherif Ramzy","doi":"10.1016/j.ab.2024.115755","DOIUrl":"10.1016/j.ab.2024.115755","url":null,"abstract":"<div><div>Magnesium is an essential mineral in biological systems and has a significant impact on brain health. Its deficiency has been found to correlate with irregular metabolic processes and neurodevelopmental disorders. The objective of this research was to establish and validate an analytical approach based on the standard addition methodology for determining endogenous magnesium levels in the serum of autistic and healthy children. Analytically, the approach involved functionalizing fluorescent graphene quantum dots with a magnesium-phosphomolybdic acid ion pair complex, followed by measuring magnesium-induced fluorescence quenching on the functionalized graphene quantum dots, which is concentration-dependent. The approach was validated in accordance with the ICH M10 requirements for bioanalytical technique validation, and it reliably quantified magnesium concentrations in the serum of both autistic and healthy children. The study found that autistic children have considerably lower serum magnesium concentrations than healthy children (<em>P</em> < 0.01), indicating a correlation between magnesium deficiency and autism spectrum disorder. The average serum magnesium levels (mg/dl) recorded for the autistic and healthy groups were 2.03 ± 0.33 and 2.28 ± 0.26, respectively.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"699 ","pages":"Article 115755"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment and MALDI-TOF-MS/MS analysis of phosphatidylinositol bisphosphates in brain tissue 脑组织中二磷酸磷脂酰肌醇的富集及MALDI-TOF-MS/MS分析。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-22 DOI: 10.1016/j.ab.2024.115749

Dinh Phi Long Nguyen , Hoa Thi Le , Dae Ho Kim , Chang-Wook Lee , Jiao Li , Choon Woo Lim , Kwang Pyo Kim , Tae Woo Kim

{"title":"Enrichment and MALDI-TOF-MS/MS analysis of phosphatidylinositol bisphosphates in brain tissue","authors":"Dinh Phi Long Nguyen , Hoa Thi Le , Dae Ho Kim , Chang-Wook Lee , Jiao Li , Choon Woo Lim , Kwang Pyo Kim , Tae Woo Kim","doi":"10.1016/j.ab.2024.115749","DOIUrl":"10.1016/j.ab.2024.115749","url":null,"abstract":"<div><div>Triazolium α-cyclodextrin click cluster-magnetic agarose bead conjugate (<sup><strong>+</strong></sup><strong>CCC-MAB</strong>) was used to enrich phosphatidylinositol bisphosphates in brain tissue. The enriched sample was phosphate-methylated and analyzed by MALDI-TOF-MS/MS in positive ion mode. <sup><strong>+</strong></sup><strong>CCC-MAB</strong> effectively removed weak-binding interferences from the phosphoinositide extract and improved the signal-to-noise ratio. The MALDI-TOF-MS/MS fragment ion revealed sodium adducts of polar head groups, exhibiting a converse fragmentation pattern compared to LC-ESI fragmentation. Our <strong><sup>+</sup>CCC-MAB</strong>-based phosphoinositide enrichment method enabled MALDI-TOF-MS/MS to assign 38 peaks in brain tissue and identify two phosphatidylinositol monophosphates, fifteen bisphosphates, and two trisphosphates. To our knowledge, this is the first study to analyze phosphatidylinositol bisphosphates in brain tissue using specific PIP enrichment and phosphate-methylation with MALDI-TOF-MS/MS.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115749"},"PeriodicalIF":2.6,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle-assisted plasmonic sensors: Recent developments in clinical applications 纳米粒子辅助等离子体传感器：临床应用的最新进展。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-22 DOI: 10.1016/j.ab.2024.115753

Duygu Çimen , Serhat Ünal , Adil Denizli

{"title":"Nanoparticle-assisted plasmonic sensors: Recent developments in clinical applications","authors":"Duygu Çimen , Serhat Ünal , Adil Denizli","doi":"10.1016/j.ab.2024.115753","DOIUrl":"10.1016/j.ab.2024.115753","url":null,"abstract":"<div><div>Nanotechnology is an important science that finds a wide range of applications from energy production to industrial production processes and biomedical applications. Nanoparti-cles, which are the most frequently preferred nanomaterials that form the basis of nanotechnolo-gy, are prepared with different composition, size, shape and surface chemistry to provide new techniques in applications in many different fields. The use of nanoparticles in the preparation of plasmonic sensors has increased the interest in plasmonic sensors such as surface plasmon resonance, electrochemical sensors, surface enhanced raman scattering and colorimetric sensors due to their increased sensing capacity on sensor surfaces. Plasmonic sensors are an important option in many different fields, such as medicine, environmental agriculture and food safety, thanks to their ability to solve a multitude of challenges. Because, plasmonic sensors are defined as sensing devices with important features such as sensitive and fast detection, no need for labels, real-time analysis, portability. In this review, the information about nanoparticles and their types and working principles of plasmonic sensors is given. Then, examples in clinical applications using different plasmonic sensors prepared with plasmonic nanoparticles are discussed in detail.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115753"},"PeriodicalIF":2.6,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico selection against progesterone receptor DNA-binding domain 黄体酮受体dna结合域和其他类固醇激素受体成员的硅筛选。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-22 DOI: 10.1016/j.ab.2024.115752

Tholasi Nadhan Navien, Ramesh Thevendran, Marimuthu Citartan

{"title":"In silico selection against progesterone receptor DNA-binding domain","authors":"Tholasi Nadhan Navien, Ramesh Thevendran, Marimuthu Citartan","doi":"10.1016/j.ab.2024.115752","DOIUrl":"10.1016/j.ab.2024.115752","url":null,"abstract":"<div><div>Progesterone receptor is one of the markers used in antibody-based immunohistochemistry for the diagnostics of breast cancer. The shortcomings of antibodies raise the need to focus on alternative molecular recognition. Aptamers are chosen due to their many advantages as compared to antibodies. However, the rigor of conventional SELEX intensifies the efforts to select DNA aptamers using <em>in silico-</em>docking approach. In this study, we performed <em>in silico</em> selection and experimental validation of DNA aptamers against the progesterone receptor DNA binding domain (PR DBD) using the ssDNA sequences derived from human progesterone response elements (PREs). Firstly, a library of sixty-four different ssDNA was subjected to secondary and tertiary structural determination prior to docking using PatchDock. PRDBDapt17 appeared to be the best candidate, with the highest docking scores of 11334. Molecular dynamic simulation also substantiates PRDBDapt17 as the most potent aptamer. This aptamer, PRDBDapt17 was validated by using direct ELASA. Direct ELASA demonstrated a limit of detection of 3.91 nM while the equilibrium dissociation constant was estimated at 366.6 nM. As PRDBDapt17 also interacts with estrogen receptor and androgen receptor, it can also be a potential universal binder of steroid hormone receptors. PRDBDapt17 can be used in the diagnostics of breast cancer.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"699 ","pages":"Article 115752"},"PeriodicalIF":2.6,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Esterase-2 mutant-based nanostructured amperometric biosensors for the selective determination of paraoxon (Neurotoxin) 基于酯酶2突变体的纳米结构安培生物传感器选择性测定对氧磷（神经毒素）。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-15 DOI: 10.1016/j.ab.2024.115751

Hend Samy Magar , Muhammad Fayez , Ferdinando Febbraio , Rabeay Y.A. Hassan

{"title":"Esterase-2 mutant-based nanostructured amperometric biosensors for the selective determination of paraoxon (Neurotoxin)","authors":"Hend Samy Magar , Muhammad Fayez , Ferdinando Febbraio , Rabeay Y.A. Hassan","doi":"10.1016/j.ab.2024.115751","DOIUrl":"10.1016/j.ab.2024.115751","url":null,"abstract":"<div><div>Organophosphate pesticides (OPs) are causing non-selective inhibition in enzymatic bioreceptors, thus the enzymatic-inhibition-based traditional assays are not suitable for their specific detection in food and environmental samples. Accordingly, a selective nanostructured electrochemical biosensing system was designed using six mutants of the esterase-2 (EST2 protein) enzymes from <em>A. acidocaldarius</em> to be exploited as targeting bio-receptors for the specific detection of OPs. Each of the EST2 mutant enzymes was immobilized on disposable screen-printed electrodes modified with Aluminum oxide (Al<sub>2</sub>O<sub>3</sub>)/Copper (Cu) nanocomposite. Consequently, chronoamperometric assay was fully optimized, and cross-reactivity study was carried out using paraoxon, malathion and chlorpyrifos. The comparative cross-reactivity study was performed on the six mutant proteins in terms of inhibitory percentage over a wide range of pesticide concentrations. Eventually, a wide dynamic inhibition range was achieved while the limit of detection for the paraoxon toxicity was 0.01 nM and the limit of quantification was 0.05 nM. Finally, paraoxon was selectively determined using the newly developed EST-based biosensor in different spiked food samples.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115751"},"PeriodicalIF":2.6,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Norovirus detection technologies: From conventional methods to innovative biosensors 诺如病毒检测技术：从传统方法到创新生物传感器。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-12 DOI: 10.1016/j.ab.2024.115750

David Septian Sumanto Marpaung , Ayu Oshin Yap Sinaga , Damayanti Damayanti

{"title":"Norovirus detection technologies: From conventional methods to innovative biosensors","authors":"David Septian Sumanto Marpaung , Ayu Oshin Yap Sinaga , Damayanti Damayanti","doi":"10.1016/j.ab.2024.115750","DOIUrl":"10.1016/j.ab.2024.115750","url":null,"abstract":"<div><div>The norovirus (NoV), known for its high contagion rate, is the leading cause of acute gastroenteritis. The development of a NoV vaccine is hindered by significant antigenic variation, lack of suitable models, unknown vaccine protection duration, limited human challenge studies, complex performance patterns, and the absence of a reliable in vitro cultivation system, making prevention, early detection, and control the only effective measures to mitigate outbreaks. This review aims to discuss about several norovirus biosensor for point-of-care analysis. Several innovative biosensors have been developed, including techniques such as electrochemical NoV biosensors, colorimetric NoV biosensors, fluorescence NoV biosensors, CRISPR-based NoV biosensors, and other NoV biosensors. These approaches have detected norovirus in biological samples with high sensitivity and specificity. This biosensing technique holds significant promise, not only in improving the speed and accuracy of diagnostic processes but also in strengthening the global response to norovirus infections, thereby underscoring its pivotal role in public health and disease prevention.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115750"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative lipidomics profiles of planktonic and biofilms of methicillin-resistant and -susceptible Staphylococcus aureus 耐甲氧西林金黄色葡萄球菌和易感金黄色葡萄球菌浮游生物和生物膜的脂质组学比较图谱。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-11 DOI: 10.1016/j.ab.2024.115746

Shilpa Saseendran Nair , Torsten Kleffmann , Briana Smith , Vanessa Morris , Christoph Göbl , Daniel Pletzer , Matthias Fellner

{"title":"Comparative lipidomics profiles of planktonic and biofilms of methicillin-resistant and -susceptible Staphylococcus aureus","authors":"Shilpa Saseendran Nair , Torsten Kleffmann , Briana Smith , Vanessa Morris , Christoph Göbl , Daniel Pletzer , Matthias Fellner","doi":"10.1016/j.ab.2024.115746","DOIUrl":"10.1016/j.ab.2024.115746","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is a significant human pathogen causing acute life-threatening, and chronic infections often linked to biofilms. This study conducted a comparative lipidomic analysis of a methicillin-resistant (MRSA) and a methicillin-susceptible (MSSA) <em>S. aureus</em> strain in both planktonic and biofilm cultures using liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy. The developed protocol successfully differentiates between the strains in various living states (planktonic and biofilm) and growth media (Tryptic Soy Broth and Brain Heart Infusion) using <em>S. aureus</em> USA300 LAC (MRSA) and <em>S. aureus</em> Newman (MSSA). LC-MS and NMR lipidomics profiles revealed global differences and particular ones among the following classes of bacterial lipids: phosphatidylglycerols, diacylglycerols, monoglycosyldiacylglycerols, diglycosyldiacylglycerols, and cardiolipins. Lipid content was higher in the biofilm states for most of these classes. Growth media differences were significant, while differences between MRSA and MSSA were less pronounced but still detectable. Additionally, we provide data on hundreds of unknown compounds that differ based on living state, strain background, or growth media. This study offer insights into the dynamic nature of <em>S. aureus</em> lipid composition and the used methods are adaptable to other organisms.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115746"},"PeriodicalIF":2.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the techniques for isolating immunoassay-suitable proteins from heterogeneous fecal samples 从异种粪便样品中分离免疫测定适宜蛋白的技术比较。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-12-10 DOI: 10.1016/j.ab.2024.115748

Subramaniam-Betty Sheila Devan , Rosli Ramli , Salah Abdalrazak Alshehade , Sharoen Yu Ming Lim , Noorhidayah Mamat

{"title":"Comparison of the techniques for isolating immunoassay-suitable proteins from heterogeneous fecal samples","authors":"Subramaniam-Betty Sheila Devan , Rosli Ramli , Salah Abdalrazak Alshehade , Sharoen Yu Ming Lim , Noorhidayah Mamat","doi":"10.1016/j.ab.2024.115748","DOIUrl":"10.1016/j.ab.2024.115748","url":null,"abstract":"<div><div>Immunoassays could provide valuable insights into disease biomarkers and gut health by measuring fecal proteins. However, reliably isolating intact proteins from feces is challenging due to its heterogeneous and variable composition. This paper aims to review and compare different methods for extracting proteins from fecal samples to make them suitable for immunoassay analysis. Mechanical homogenization helps release proteins by disrupting solids, while protease inhibitors preserve protein integrity. Detergents like SDS solubilize proteins by disrupting hydrophobic interactions. Organic solvents such as acetone precipitate proteins and remove contaminants. Thermal treatment denatures proteases. Immunocapture uses antibodies to purify target proteins away from interference selectively. Commercial kits contain optimized buffers but may be cost-prohibitive. Combining mechanical, chemical, and immunological techniques synergistically integrates their advantages, improving the recovery of native proteins with reduced matrix effects. While all methods have merits, tailored protocols integrating multiple mechanisms appear most promising for extracting immunoassay-suitable fecal proteins. Further optimization and standardization of such combination approaches matched to proteins and assays of interest helps expand noninvasive fecal proteome analysis.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"698 ","pages":"Article 115748"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0