Analytical biochemistry最新文献_第8页

Real-time electrochemical detection of Ascorbic acid in mouse brain homogenate using SCN-wrapped Co@NiO Nanosensor scn包覆Co@NiO纳米传感器实时电化学检测小鼠脑匀浆中抗坏血酸。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-09-17 DOI: 10.1016/j.ab.2025.115980

Shahid Habib Ansari , Sana Amjad , Tehreem Ul Wara , Sehrish Hanif , Ali Raza , Hafiza Khushbakht Hussain , Naeem Akhtar , Mohibullah Shah , Imran Imran , Sonam Javaid Khan , Bushra Yaqub , Samra Rasheed

{"title":"Real-time electrochemical detection of Ascorbic acid in mouse brain homogenate using SCN-wrapped Co@NiO Nanosensor","authors":"Shahid Habib Ansari , Sana Amjad , Tehreem Ul Wara , Sehrish Hanif , Ali Raza , Hafiza Khushbakht Hussain , Naeem Akhtar , Mohibullah Shah , Imran Imran , Sonam Javaid Khan , Bushra Yaqub , Samra Rasheed","doi":"10.1016/j.ab.2025.115980","DOIUrl":"10.1016/j.ab.2025.115980","url":null,"abstract":"<div><div>Oxidative stress, a major key factor to neurological disorders such as Parkinson's, Alzheimer's, and Huntington's disease. Ascorbic acid (AA), a vital brain antioxidant, protects neurons by scavenging reactive oxygen species, and its fluctuations can damage neuronal function. Therefore, precise monitoring of AA in humans is critical for early diagnosis and disease management. However, despite significant advancements in noble metal-free nanocomposite based electrochemical sensors, precise detection remains challenging due to low physiological concentrations and interference from coexisting biomolecules, which limit sensor sensitivity, selectivity, and real-time applicability. To address these limitations herein, we synthesized cobalt-doped nickel oxide (Co@NiO), wrapped with thiourea, and dopamine (SCN- wrapped Co@NiO) for selective and sensitive detection of AA. The SCN groups provide abundant binding sites through hydrogen-bonding interactions with AA, thereby enhancing selectivity, while the Co@NiO nanostructure catalytically facilitates AA oxidation, significantly improving sensitivity and enabling efficient sensing efficacy against interfering species. The findings demonstrated that the fabricated material exhibits excellent sensitivity (0.1837 μA/nM/cm<sup>2</sup>), a wide linear range (5nM–20μM), and low detection limit (12.72 nM). Furthermore, the designed electrode has shown excellent selectivity towards AA even in presence of various interfering species, highlighting its potential in real-time analysis for practical applications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115980"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterodimeric aptamer improves the limit of detection of sandwich ELASA and dot blot assay 异二聚体提高了夹心ELASA和斑点印迹法的检出限。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-09-25 DOI: 10.1016/j.ab.2025.115984

Tzi Shien Yeoh, Marimuthu Citartan

引用次数: 0

High-throughput protein A chromatography platform for accurate antibody quantification in IV admixtures 高通量蛋白A层析平台用于IV外加剂中抗体的准确定量。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-09-18 DOI: 10.1016/j.ab.2025.115982

Tse-Hong Chen, Xiaoyang Liu, Shao-Chun Wang, Mohammed Shameem, Kenneth S. Graham

{"title":"High-throughput protein A chromatography platform for accurate antibody quantification in IV admixtures","authors":"Tse-Hong Chen, Xiaoyang Liu, Shao-Chun Wang, Mohammed Shameem, Kenneth S. Graham","doi":"10.1016/j.ab.2025.115982","DOIUrl":"10.1016/j.ab.2025.115982","url":null,"abstract":"<div><div>Antibodies hold significant therapeutic potential, but accurate quantification of them in complex intravenous (IV) admixtures is critical for ensuring therapeutic efficacy and patient safety. Traditional reversed-phase liquid chromatography (RPLC) often faces challenges in resolving antibodies from non-product related impurities within IV matrices. This study first evaluates the specificity of Protein A (ProA) affinity chromatography for quantifying bispecific antibodies (BsAbs) in IV matrices. The method leverages the selective binding of ProA to the antibody Fc region to achieve clear separation of antibodies from potential interference, including human serum albumin (HSA), and extractables and leachables (E&L) from contact materials, enabling accurate quantification down to 0.1 μg/mL. The fit-for-purpose method qualification secondly demonstrates linearity, accuracy, and precision across the tested concentration ranges. The ProA method achieves rapid and complete separation in 5 min without sample preparation, significantly enhancing throughput. This platform capability is further demonstrated by its successful application to five different BsAbs in this study. These findings highlight the ProA method as a reliable, efficient, and specific approach for accurately quantifying antibodies in the presence of challenging IV admixture matrices, supporting the development and administration of low-dose antibody therapies.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115982"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H-ferritin nanocages: refined strategy for optimized protein production and purification h -铁蛋白纳米笼：优化蛋白质生产和纯化的精制策略。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-09-25 DOI: 10.1016/j.ab.2025.115983

Arianna Bonizzi, Marta Sevieri, Leopoldo Sitia, Raffaele Allevi, Francesca Gorgoglione, Ilaria Tagliolini, Beatrice Bignami, Serena Mazzucchelli

引用次数: 0

Biosynthesised reduced graphene oxide/CuO based nanocomposite using ‘Cordia dichotoma’ leaf extract for ‘nitrobenzene’ determination 生物合成还原氧化石墨烯/CuO基纳米复合材料，使用“Cordia dichotoma”叶提取物测定“硝基苯”

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-09-10 DOI: 10.1016/j.ab.2025.115972

Devkumari Patel , Robind Kumar , Sanju Yadav , Ankita Rai , Vijai K. Rai , Manorama Singh

{"title":"Biosynthesised reduced graphene oxide/CuO based nanocomposite using ‘Cordia dichotoma’ leaf extract for ‘nitrobenzene’ determination","authors":"Devkumari Patel , Robind Kumar , Sanju Yadav , Ankita Rai , Vijai K. Rai , Manorama Singh","doi":"10.1016/j.ab.2025.115972","DOIUrl":"10.1016/j.ab.2025.115972","url":null,"abstract":"<div><div>Herein, plant-assisted synthesis of copper oxide nanoparticles (CuO NPs)<sub>CD</sub> and reduced graphene oxide (CDrGO) was performed individually using the leaf extract of ‘<em>Cordia dichotoma’</em> plant and they were associated with electrochemically active conducting clay ‘Sodium-Montmorillonite (Na-MT)’ leading to the synthesis of a new nanocomposite named ‘(CuO NPs)<sub>CD</sub>@CDrGO-Na-MT’. The collaboration of CDrGO paired (CuO NPs)<sub>CD</sub> and Na-MT enhanced the electrochemical reduction of noxious ‘Nitrobenzene (NB)’ at a lower potential. The prepared ternary nanocomposite was confirmed with characterisation techniques, Transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Electrochemical properties and electrochemical reduction of ‘Nitrobenzene’ were inspected with cyclic voltammetry and differential pulse voltammetry techniques, respectively. The electro-reduction of NB was recorded in two wide linear ranges of 0.016–360 μM and 360–6980 μM with a detection limit of 15 nM and negligible effect of interferences.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115972"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of concern: “Specific detection of Mycobacterium sp. genomic DNA using dual labeled gold nanoparticle based electrochemical biosensor” [Anal. Biochem. 417 (1) (2011) 73-79] 关注表达：“使用基于双标记金纳米粒子的电化学生物传感器特异性检测分枝杆菌基因组DNA”[肛。生物化学，417 (1)(2011)73-79]

IF 3.1 4区生物学

Analytical biochemistry Pub Date : 2026-01-01

引用次数: 0

High-performance Sn/AuNP-based spectroscopic ellipsometric sensor for patulin 基于Sn/ aunp的展青霉素光谱椭偏传感器。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-03 DOI: 10.1016/j.ab.2025.115987

Zafer Üstündağ , Sinem Tunçer Çağlayan , Mustafa Oguzhan Caglayan

{"title":"High-performance Sn/AuNP-based spectroscopic ellipsometric sensor for patulin","authors":"Zafer Üstündağ , Sinem Tunçer Çağlayan , Mustafa Oguzhan Caglayan","doi":"10.1016/j.ab.2025.115987","DOIUrl":"10.1016/j.ab.2025.115987","url":null,"abstract":"<div><div>A label-free spectroscopic ellipsometric (SE) aptasensor was developed for ultra-sensitive patulin (PAT) detection on a tin (Sn) substrate modified via electrochemical grafting of a 4-mercaptobenzene diazonium-derived self-assembled monolayer. Cyclic voltammetry and high-resolution X-ray photoelectron spectroscopy confirmed uniform nanofilm formation, followed by citrate-reduced gold nanoparticle decoration and thiolated anti-PAT aptamer immobilization. Monitoring the ellipsometric phase shift (Δ) at 507 nm and 508 nm yielded linear responses over 0.01–1000 ng/mL PAT (R<sup>2</sup> > 0.98) with limits of detection of 10.7 pg/mL and 9.5 pg/mL (3.3 σ), respectively. Isotherm fitting indicated pseudo-second-order binding (R<sup>2</sup> = 1.00), consistent with surface-constrained, mass-transfer-limited interactions. Precision (%RSD <1.1 %) and accuracy (%RE within ±5 %) were validated in both buffer and spiked apple-juice samples (recoveries 98.9–103.2 %). This label-free SE approach combines a wide dynamic range and sub-10 pg/mL sensitivity with minimal sample preparation and no external labels.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115987"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultrasensitive electrochemical immunosensor constructed via Ag-GO-Nf nanocomposites for the detection of pefloxacin Ag-GO-Nf纳米复合材料构建的超灵敏培氟沙星电化学免疫传感器。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-03 DOI: 10.1016/j.ab.2025.115985

Jiaojiao Zhang , Yankai Liu , Enping Liu , Yajiao Li , Jingming Zhou , Yumei Chen , Hongliang Liu , Xifang Zhu , Aiping Wang

{"title":"An ultrasensitive electrochemical immunosensor constructed via Ag-GO-Nf nanocomposites for the detection of pefloxacin","authors":"Jiaojiao Zhang , Yankai Liu , Enping Liu , Yajiao Li , Jingming Zhou , Yumei Chen , Hongliang Liu , Xifang Zhu , Aiping Wang","doi":"10.1016/j.ab.2025.115985","DOIUrl":"10.1016/j.ab.2025.115985","url":null,"abstract":"<div><div>Pefloxacin (PEF), a third-generation quinolone antimicrobial drug, is widely used in fisheries and animal husbandry. However, overuse poses food safety hazards and serious threats to human health. In this study, a label-free electrochemical immunosensor based on Ag-GO-Nf nanocomposites was developed for the rapid detection of PEF. Although electrochemical immunoassays are well-established for quinolone detection, no specific method has been reported for PEF. We synthesized silver nanoparticle/graphene oxide/Nafion nanocomposites (Ag-GO-Nf) via in situ reduction, where the synergistic effect of GO (high conductivity) and AgNPs (redox-active) significantly enhanced electron transfer efficiency. The nanocomposites were characterised by UV–Vis, XRD, SEM, and TEM, while electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were employed to monitor the stepwise immunosensor assembly. Key experimental parameters were optimized systematically. The developed sensor exhibited a wide linear range (0.50–100 ng/mL), an ultra-low limit of detection (0.27 ng/mL, S/N = 3), and high specificity toward structural analogs. Practical assays in spiked pork and milk samples yielded recoveries of 97.0–100.4 %, while the current signal retained 90.77 % of its initial value after 28 days, demonstrating excellent long-term stability. This work provides a robust technical strategy for rapid, sensitive, and efficient PEF detection.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115985"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3DCOOR-Kace: A 3-d spatial coordinates representation method for lysine acetylation site identification 一种用于赖氨酸乙酰化位点识别的三维空间坐标表示方法。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-16 DOI: 10.1016/j.ab.2025.115994

Lichao Zhang , Xue Wang , Liang Kong

{"title":"3DCOOR-Kace: A 3-d spatial coordinates representation method for lysine acetylation site identification","authors":"Lichao Zhang , Xue Wang , Liang Kong","doi":"10.1016/j.ab.2025.115994","DOIUrl":"10.1016/j.ab.2025.115994","url":null,"abstract":"<div><div>Lysine acetylation (Kace) is an important post-translational modification. Although structure information has been proven to be a key for improving model effectiveness, it is difficult to obtain in bulk due to experiments limitations. In this study, we propose a spatial coordinates representation using a 2-order tensor according to defined property sequence to address the existing limitations and depicting amino acid position in 3-d space. Based on the proposed coordinates, we construct optimal complex networks to extract network-derived features. Compared to existing network construction methods, protein contact networks (PCN), the features achieve superior performance, demonstrating the proposed spatial coordinates could effectively capture biological global information. Meanwhile, we proposed a computational model, named 3DCOOR-Kace, by fusing sequence and structure information based on DenseNet and Squeeze-and-Excitation layer. The 3DCOOR-Kace achieved satisfactory MCC with 0.7358. Compared with MusiteDeep and TransPTM by the independent testing set, the MCC is 0.4261 higher than MusiteDeep and 0.1660 higher than TransPTM, which demonstrates 3DCOOR-Kace are effective for integrating structure and sequence information for improving Kace site identification. Instead of doing biological experiments, the 3-d spatial coordinates representation could give sites positions directly, which could address the experiments limitation and be convenient for computational methods and biological function research.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"708 ","pages":"Article 115994"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the mode of action of arsenic(III) on the fungus Neofusicoccum parvum: Target protein identification 了解砷（III）对新褐菌的作用模式：靶蛋白鉴定。

IF 2.5 4区生物学

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-08 DOI: 10.1016/j.ab.2025.115988

Andréa Engel , Florence Ferrari , Maude Meyer , Jean-Marc Strub , Martin Spichty , Christophe Bertsch , Christine Schaeffer-Reiss , Céline Tarnus , Sébastien Albrecht , Mary-Lorène Goddard

引用次数: 0