Metabolomics approach using HR-MAS NMR spectroscopy for the assessment of metabolic profiles of uterine fibroids

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-04-29 DOI:10.1016/j.ab.2025.115885

Senem Arda Düz , Akın Mumcu , Berat Doğan , Erdinç Sarıdoğan , Görkem Tuncay , Taylan Onat , Abdullah Karaer

{"title":"Metabolomics approach using HR-MAS NMR spectroscopy for the assessment of metabolic profiles of uterine fibroids","authors":"Senem Arda Düz , Akın Mumcu , Berat Doğan , Erdinç Sarıdoğan , Görkem Tuncay , Taylan Onat , Abdullah Karaer","doi":"10.1016/j.ab.2025.115885","DOIUrl":null,"url":null,"abstract":"<div><div>The aim of this study is to determine dysregulated metabolites and metabolic pathways in uterine fibroids and in the myometrial tissue from which uterine fibroids are derived. Fifteen (15) patients underwent hysterectomy because of uterine fibroids and 14 controls were included in this study. <sup>1</sup>H HR-MAS NMR spectroscopy data were obtained from uterine fibroid tissue, the adjacent healthy myometrial tissue from cases, and myometrial tissue from controls. PCA and PLS-DA score plots from multivariate statistical analysis of pre-processed spectral data demonstrated a distinction between cases and control groups. The levels of lactate, alanine, glutamate, glutamine, methionine, acetone, isocitrate, choline, glycerophosphocholine, phosphocholine, <em>o</em>-phosphoethanolamine, taurine, myo-inositol, <em>p</em>-methylhistidine, phenylacetate, ascorbate, glucose, and methylhistidine were significantly higher in uterine fibroid tissue compared to the neighboring healthy myometrial tissue. Additionally, when adjacent healthy myometrial tissue was compared to control myometrial tissue, significantly lower levels of valine, leucine, isoleucine, ethanol, arginine, N-acetyl tyrosine, acetone, <em>p</em>-methylhistidine, glucose, phenylacetate, myo-inositol, and alpha-glucose were observed. The study provides a foundational framework by revealing the metabolomic heterogeneity of uterine fibroids. Strategies should be developed to target the metabolic alterations that contribute to the growth of these common tumors.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"704 ","pages":"Article 115885"},"PeriodicalIF":2.6000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S000326972500123X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of this study is to determine dysregulated metabolites and metabolic pathways in uterine fibroids and in the myometrial tissue from which uterine fibroids are derived. Fifteen (15) patients underwent hysterectomy because of uterine fibroids and 14 controls were included in this study. ¹H HR-MAS NMR spectroscopy data were obtained from uterine fibroid tissue, the adjacent healthy myometrial tissue from cases, and myometrial tissue from controls. PCA and PLS-DA score plots from multivariate statistical analysis of pre-processed spectral data demonstrated a distinction between cases and control groups. The levels of lactate, alanine, glutamate, glutamine, methionine, acetone, isocitrate, choline, glycerophosphocholine, phosphocholine, o-phosphoethanolamine, taurine, myo-inositol, p-methylhistidine, phenylacetate, ascorbate, glucose, and methylhistidine were significantly higher in uterine fibroid tissue compared to the neighboring healthy myometrial tissue. Additionally, when adjacent healthy myometrial tissue was compared to control myometrial tissue, significantly lower levels of valine, leucine, isoleucine, ethanol, arginine, N-acetyl tyrosine, acetone, p-methylhistidine, glucose, phenylacetate, myo-inositol, and alpha-glucose were observed. The study provides a foundational framework by revealing the metabolomic heterogeneity of uterine fibroids. Strategies should be developed to target the metabolic alterations that contribute to the growth of these common tumors.

Abstract Image

查看原文本刊更多论文

代谢组学方法使用HR-MAS核磁共振光谱评估子宫肌瘤的代谢谱

本研究的目的是确定子宫肌瘤和子宫肌瘤来源的肌层组织中代谢异常的代谢物和代谢途径。本研究包括15例因子宫肌瘤而行子宫切除术的患者和14例对照组。1H HR-MAS NMR数据分别取自子宫肌瘤组织、病例的相邻健康子宫肌组织和对照组的子宫肌组织。预处理光谱数据的PCA和PLS-DA评分图显示了病例与对照组之间的差异。子宫肌瘤组织中乳酸、丙氨酸、谷氨酸、谷氨酰胺、蛋氨酸、丙酮、异柠檬酸、胆碱、甘油磷胆碱、磷胆碱、邻磷乙醇胺、牛磺酸、肌肌醇、对甲基组氨酸、苯乙酸、抗坏血酸、葡萄糖和甲基组氨酸的水平明显高于邻近的健康子宫肌瘤组织。此外，当邻近的健康肌层组织与对照肌层组织进行比较时，观察到缬氨酸、亮氨酸、异亮氨酸、乙醇、精氨酸、n -乙酰酪氨酸、丙酮、对甲基组氨酸、葡萄糖、苯乙酸酯、肌醇和α -葡萄糖的水平显著降低。该研究通过揭示子宫肌瘤的代谢组异质性提供了一个基础框架。应该制定策略来针对导致这些常见肿瘤生长的代谢改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.