Analytical biochemistry最新文献_第7页

Univariate versus multivariate approaches for resolving the overlapped spectra of azelastine hydrochloride and mometasone furoate 单变量与多变量方法分析盐酸氮唑elastine和糠酸莫米松的重叠光谱。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-08 DOI: 10.1016/j.ab.2025.115902

Alaa Reda , Hawa Khalil , Eman A. Bahgat , Michael Gamal Fawzy

{"title":"Univariate versus multivariate approaches for resolving the overlapped spectra of azelastine hydrochloride and mometasone furoate","authors":"Alaa Reda , Hawa Khalil , Eman A. Bahgat , Michael Gamal Fawzy","doi":"10.1016/j.ab.2025.115902","DOIUrl":"10.1016/j.ab.2025.115902","url":null,"abstract":"<div><div>Azelastine hydrochloride (AZE) and Mometasone furoate (MOM) combination is used to treat allergic rhinitis' symptoms. The aim of this work is to qualitatively and quantitatively analyze both medications using univariate and multivariate spectrophotometric techniques in a comparative study. Regarding univariate approaches; AZE was quantified by direct measurement at 291 nm within (5–60 μg/mL) concentration range. While, MOM was assayed by absorption correction (AC) approach at 250 nm within the range of (2–18 μg/mL). The LOD values for AZE and MOM were (0.79 μg/mL) and (0.21 μg/mL), respectively. Classical least squares (CLS), partial least squares (PLS), principal component regression (PCR), multivariate curve resolution-alternating least squares (MCR-ALS) and artificial neural networks (ANN) were the applied multivariate chemometric models. The proposed methods were utilized for analyzing the binary mixture in laboratory-synthetic mixtures and pharmaceutical preparation with correlation coefficients values ≥ 0.9996. No statistically significant variation was found between the applied methods and the reported HPLC one. The methods’ sustainability was assessed using blue applicability grade index (BAGI) and Red-Green-Blue 12 (RGB12) metrics. The obtained findings revealed that the suggested methodologies are safer option than the published HPLC technique for the conventional pharmaceutical analysis of the studied medications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"704 ","pages":"Article 115902"},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Absolute quantitation of neopterin as an endogenous pharmacodynamic Biomarker: The successful method development, validation, and use of a surrogate matrix for clinical sample analysis 新蝶呤作为内源性药效学生物标志物的绝对定量：成功的方法开发、验证和临床样品分析替代基质的使用

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-07 DOI: 10.1016/j.ab.2025.115893

Ryan De Palma, Murali Matta, Jeffry Florian, Vikram Patel, Rodney Rouse

{"title":"Absolute quantitation of neopterin as an endogenous pharmacodynamic Biomarker: The successful method development, validation, and use of a surrogate matrix for clinical sample analysis","authors":"Ryan De Palma, Murali Matta, Jeffry Florian, Vikram Patel, Rodney Rouse","doi":"10.1016/j.ab.2025.115893","DOIUrl":"10.1016/j.ab.2025.115893","url":null,"abstract":"<div><div>Biomarkers are playing an increasing role in the drug discovery and drug development process. Molecular biomarkers pose a bioanalytical challenge due to their low concentrations and endogenous presence. Inaccurate quantitation could lead to biased study results. Surrogate analyte and surrogate matrix approaches can be used to overcome the lack of a blank matrix and provide accurate quantitation. However, the suitability of the surrogate analyte or matrix must be established during method validation. Here we describe the development and validation of a surrogate matrix approach for the absolute quantitation of neopterin as a PD biomarker for use in an FDA-sponsored clinical study (NCT04183491). Matrix suitability was established through parallelism, precision and accuracy, and internal standard response. Parallelism experiments showed FBS, and serum had identical slopes 0.0145. Additionally, the difference in the X-intercepts was able to accurately predict the amount of endogenous neopterin (1.0 ng/mL). Inter-day accuracy across four surrogate QC levels ranged from 92.08 to 109.06 % while precision ranged from 3.36 to 16.00 %. Inter-day accuracy for the QCs in study matrix ranged from 96.81 to 108.86 % and precision ranged from 4.13 to 6.01 %. The internal standard response in FBS was only 6.9 % different from serum. Additionally, there was no matrix effect, injection carryover, or cross-analyte interference observed. The method was then qualified for automated sample processing.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"704 ","pages":"Article 115893"},"PeriodicalIF":2.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Identifying transglutaminase substrate glutaminyls using dansylcadaverine

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-07 DOI: 10.1016/j.ab.2025.115888

Thomas M. Jeitner , Pradeep K. Singh , Juan Azcona , Chad W. Euler , James M. Kelly

引用次数: 0

Double alkylation with maleimide-PEG-biotin: An enrichment method for cysteine redox states 马来酰亚胺-聚乙二醇-生物素双烷基化：半胱氨酸氧化还原态的富集方法

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-06 DOI: 10.1016/j.ab.2025.115892

Jung Mi Lim , Rodney L. Levine

引用次数: 0

Polyaniline-graphene oxide (PANI/GO)-grafted paper-based nanosensor for the detection of Helicobacter pylori 聚苯胺-氧化石墨烯接枝纸基幽门螺杆菌检测传感器

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-05 DOI: 10.1016/j.ab.2025.115891

Rachna Poria , Desmond Lutomia , Ankur Kaushal , Selva Kumar Ramasamy , Shagun Gupta

{"title":"Polyaniline-graphene oxide (PANI/GO)-grafted paper-based nanosensor for the detection of Helicobacter pylori","authors":"Rachna Poria , Desmond Lutomia , Ankur Kaushal , Selva Kumar Ramasamy , Shagun Gupta","doi":"10.1016/j.ab.2025.115891","DOIUrl":"10.1016/j.ab.2025.115891","url":null,"abstract":"<div><div>A polyaniline-graphene oxide (PANI-GO) nanocomposite-grafted DNA biosensor for detecting <em>Helicobacter pylori</em>-specific toxins, oncoprotein cytotoxin-associated gene A (<em>CagA</em>), has been reported. The nanocomposite was fabricated on Screen printed paper electrode (SPPE) and modified with a 5′NH<sub>2</sub>-labelled single-stranded DNA (ssDNA) probe specific to the <em>CagA</em> gene via an EDC/NHS cross-linker. Under optimized electrochemical experimental conditions, CV and DPV were used to analyse the performance of the developed biosensor. A linear dynamic range for <em>H. pylori</em> ssDNA was established between 0.00001 ng/μl and 0.1 ng/μl, with correlation coefficients of R<sup>2</sup> = 0.9813 for the CV and R<sup>2</sup> = 0.9343 for the DPV. The sensitivities of the developed sensor in the CV studies were 50.261 μA μL/ng∙mm<sup>2</sup> and 66.5 μA μL/ng∙mm<sup>2</sup> in the DPV studies. CV demonstrated an LOD of 0.0026 ng/μL, whereas the LOD of the DPV studies was 0.001 ng/μL. The developed sensor was validated using different concentrations of <em>H. pylori</em> ssDNA spiked in human stool samples. The results highlight the potential of the developed biosensor to detect and quantify <em>H. pylori</em> genomic DNA in a sensitive and reliable manner to aid in clinical diagnostics and pathogen detection applications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"704 ","pages":"Article 115891"},"PeriodicalIF":2.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A beginners guide to SELEX and DNA aptamers SELEX和DNA适体的初学者指南

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-02 DOI: 10.1016/j.ab.2025.115890

Cameron Stephens, Nina M. Goodey, Ueli Gubler

{"title":"A beginners guide to SELEX and DNA aptamers","authors":"Cameron Stephens, Nina M. Goodey, Ueli Gubler","doi":"10.1016/j.ab.2025.115890","DOIUrl":"10.1016/j.ab.2025.115890","url":null,"abstract":"<div><div>SELEX stands for \"Systematic Evolution of Ligands by Exponential Enrichment.” It is an <em>in vitro,</em> iterative, PCR-based, target-specific selection strategy used to generate single-stranded DNA (ssDNA) aptamers that bind a target of interest. Properly selected aptamers bind their targets with high affinity and specificity and have utility in a multitude of detection assays. They are thus similar to antibodies but have the advantage of being more stable and cheaper to produce. The SELEX process encompasses several steps, some of which are critical to the successful isolation of an aptamer. Careful analysis and optimization of the SELEX process are thus important. This review summarizes our own experience when we, as complete novices, were setting up the SELEX system in our lab. It is thus meant to give some general and practical but concise pointers for anyone interested in initiating their own SELEX experiments. As such, the review covers key elements of the SELEX process, including library design, target selection and immobilization strategies, aptamer binding conditions, partitioning techniques, and PCR optimization. We also discuss common pitfalls such as by-product formation and single-stranded DNA recovery challenges, along with practical strategies to overcome them. Emerging trends and post-SELEX considerations, such as sequencing, structure prediction, and chemical modifications, are included to guide beginners through every stage of aptamer development.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"703 ","pages":"Article 115890"},"PeriodicalIF":2.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of DNA glycoxidation by mannitol and Pyridoxamine: Implications for DNA-antibody development in diabetes and diabetic retinopathy 甘露醇和吡哆胺对DNA糖氧化的抑制作用：对糖尿病和糖尿病视网膜病变中DNA抗体发展的影响

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-02 DOI: 10.1016/j.ab.2025.115886

Rihab Akasha , Uzma Shahab , Ramendra Pati Pandey , Saif Khan , Paridhi Puri , Zeeshan Rafi , Sultan Alouffi , Saheem Ahmad

{"title":"Inhibition of DNA glycoxidation by mannitol and Pyridoxamine: Implications for DNA-antibody development in diabetes and diabetic retinopathy","authors":"Rihab Akasha , Uzma Shahab , Ramendra Pati Pandey , Saif Khan , Paridhi Puri , Zeeshan Rafi , Sultan Alouffi , Saheem Ahmad","doi":"10.1016/j.ab.2025.115886","DOIUrl":"10.1016/j.ab.2025.115886","url":null,"abstract":"<div><div>Chronic exposure to reactive carbonyl species such as glyoxal and methylglyoxal, along with hydroxyl radicals (<sup>•</sup>OH), leads to glycative and oxidative damage, contributing to insulin resistance and diabetic complications. Pyridoxamine (PM) is known to counteract these effects, but its potential synergy with mannitol (MN), a hydroxyl radical scavenger, remains unexplored. This study investigates the combined efficacy of MN and PM in preventing glycation and oxidative damage in vitro.</div><div>Calf thymus DNA was subjected to glycation using 10 mM glyoxal, oxidation via the Fenton reaction, and sequential glycoxidation (glycation followed by oxidation). The inhibitory effects of MN, PM, and their combination were assessed using NBT reduction for early glycation, GK-ribose for AGEs, TBARS for hydroxyl radicals, and spectroscopic analyses for AGEs formation. A clinical study also examined autoantibody prevalence in diabetes and diabetic retinopathy (DR).</div><div>Results showed that glycoxidated DNA exhibited structural alterations, with MN and PM individually reducing ketoamine content. Their combination further enhanced glycation and glycoxidation inhibition. Additionally, MN-PM co-administration synergistically reduced AGEs and hydroxyl radicals. Autoantibody levels were elevated in diabetes and DR. These findings suggest PM-MN co-administration as a promising strategy to mitigate diabetic complications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"703 ","pages":"Article 115886"},"PeriodicalIF":2.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Development of a chemiluminescence enzyme immunoassay (CLEIA) for quantitating L1 protein in HPV vaccines

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-02 DOI: 10.1016/j.ab.2025.115889

ChengRui Fei , Huan Yang , SiJie Wang , WenZhi He , Xue Shen , Ying Zhang , YiHeng Jiang , Li Yang , XiaoJuan Li , Fan Wu , YaNan Wu , Qin Liu

{"title":"Development of a chemiluminescence enzyme immunoassay (CLEIA) for quantitating L1 protein in HPV vaccines","authors":"ChengRui Fei , Huan Yang , SiJie Wang , WenZhi He , Xue Shen , Ying Zhang , YiHeng Jiang , Li Yang , XiaoJuan Li , Fan Wu , YaNan Wu , Qin Liu","doi":"10.1016/j.ab.2025.115889","DOIUrl":"10.1016/j.ab.2025.115889","url":null,"abstract":"<div><div>The L1 protein serves as the principal capsid component of human papillomavirus (HPV). All globally commercialized HPV vaccines utilize virus-like particles (VLPs) formed through L1 protein self-assembly. Quantitative analysis of L1 protein concentration constitutes a critical parameter for evaluating the antigenic potency of HPV vaccines. In this study, we developed a chemiluminescent enzyme immunoassay (CLEIA) for precise quantification of L1 protein in bivalent HPV vaccines. Employing a sandwich immunoassay format, antigen-antibody complexes were immobilized on 96-well microplates using capture antibodies, followed by detection with HRP-conjugated secondary antibodies. Chemiluminescent signal amplification was achieved through enzymatic catalysis of luminol/hydrogen peroxide in the presence of enhancer molecules. Systematic optimization of experimental parameters yielded a validated methodology demonstrating excellent reproducibility (inter-assay CV < 4 %), accuracy (recovery rate 100.5 ± 2.8 % for 16L1 and 95.5 ± 6.4 % for 18L1), precision (inter-assay CV < 7 %) and sensitivity (limit of quantitation (LOQ) 0.0996 μg/mL for HPV16L1 and 0.1459 μg/mL for HPV18L1). This optimized assay provides a reliable analytical platform for quantitating L1 Protein in bivalent HPV vaccine.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"704 ","pages":"Article 115889"},"PeriodicalIF":2.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple DNAzyme cleavage mediated signal cascade for sensitive and reliable Kawasaki disease related microRNA analysis 三重DNAzyme切割介导的信号级联用于敏感可靠的川崎病相关microRNA分析

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-05-01 DOI: 10.1016/j.ab.2025.115887

Qunyan Ruan, Bina Zhao

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TransCNN: A novel architecture combining transformer and TextCNN for detecting N4-acetylcytidine sites in human mRNA transnn：一种结合transformer和TextCNN的新型结构，用于检测人类mRNA中的n4 -乙酰胞苷位点

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-04-29 DOI: 10.1016/j.ab.2025.115882

Shengli Zhang, Kai Liu, Yujie Xu

{"title":"TransCNN: A novel architecture combining transformer and TextCNN for detecting N4-acetylcytidine sites in human mRNA","authors":"Shengli Zhang, Kai Liu, Yujie Xu","doi":"10.1016/j.ab.2025.115882","DOIUrl":"10.1016/j.ab.2025.115882","url":null,"abstract":"<div><div>N4-acetylcytidine (ac4C), a pivotal post-transcriptional RNA modification, is central to understanding transcriptional regulation and diverse biological processes. As a key determinant of RNA structural stability and functional regulation, ac4C has been strongly associated with multiple human diseases. We can obtain a better understanding of regulation mechanism of gene expression by identifying ac4C sites rapidly and precisely. However, existing predictive approaches are constrained by limitations in feature representation and sequence context modeling, necessitating the development of advanced methodologies. In this study, we introduce a novel architecture named TransCNN that integrates transformer and Text convolutional neural network (TextCNN) to predict ac4C sites. TransCNN demonstrates superior performance compared to existing models on both 10-fold cross-validation and independent dataset with the accuracy of 83.27 % and 82.89 %, respectively. The enhanced performance of TransCNN is attributed to the transformer's ability to extract adaptive features and TextCNN's capability to form both narrow and broad connections within the sequence. This study aims to contribute significantly to the field by advancing the understanding and prediction of RNA modifications. The datasets and code used in this study are available at <span><span>https://github.com/liukai23157/</span><svg><path></path></svg></span>TransCNN.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"703 ","pages":"Article 115882"},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0