Analytical biochemistry最新文献_第7页

Integrative biophysical characterization of molecular interactions: A case study with the sfGFP–nanobody complex 分子相互作用的综合生物物理表征：sfgfp -纳米体复合物的案例研究

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-04-07 DOI: 10.1016/j.ab.2025.115859

Aysha K. Demeler , James Bosco , Matthew J. Sydor , Michelle Nemetchek , Saeed Mortezazadeh , Liam Kerr , Sophia Bird , Roza Gabdullina , Cindee Yates-Hansen , Levi J. McClelland , Ekaterina Voronina , Trushar R. Patel , Borries Demeler

{"title":"Integrative biophysical characterization of molecular interactions: A case study with the sfGFP–nanobody complex","authors":"Aysha K. Demeler , James Bosco , Matthew J. Sydor , Michelle Nemetchek , Saeed Mortezazadeh , Liam Kerr , Sophia Bird , Roza Gabdullina , Cindee Yates-Hansen , Levi J. McClelland , Ekaterina Voronina , Trushar R. Patel , Borries Demeler","doi":"10.1016/j.ab.2025.115859","DOIUrl":"10.1016/j.ab.2025.115859","url":null,"abstract":"<div><div>This study compares several analytical biophysical methods for investigating protein-protein interactions (PPIs) in solution, using the interaction between superfolder green fluorescent protein (sfGFP) and its anti-sfGFP nanobody enhancer as a model system. Techniques evaluated include microscale thermophoresis, fluorescence correlation spectroscopy, analytical ultracentrifugation with multi-wavelength and fluorescence detection, isothermal titration calorimetry, and analytical size exclusion chromatography coupled to multi-angle static light scattering and dynamic light scattering. Each method was assessed for information content, dynamic range, precision, and complementarity. The results consistently indicate a single-digit nanomolar dissociation constant and 1:1 stoichiometry for the interaction. While each technique offers unique insights into binding affinity, thermodynamics, and stoichiometry of the interaction, the multi-method approach provides a more complete and reliable characterization of PPIs. The study demonstrates how combining multiple complementary techniques enhances the robustness of PPI analysis in solution-phase conditions.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"703 ","pages":"Article 115859"},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE 凝胶内再折叠允许在SDS-PAGE后对完全变性的gfp进行荧光检测

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-04-05 DOI: 10.1016/j.ab.2025.115861

Misa Shiratori , Rio Tsuyuki , Miwako Asanuma , Saki Kawabata , Hiromasa Yoshioka , Kenji Ohgane

{"title":"In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE","authors":"Misa Shiratori , Rio Tsuyuki , Miwako Asanuma , Saki Kawabata , Hiromasa Yoshioka , Kenji Ohgane","doi":"10.1016/j.ab.2025.115861","DOIUrl":"10.1016/j.ab.2025.115861","url":null,"abstract":"<div><div>Green fluorescent proteins (GFPs) have been widely used as fusion tags, especially to visualize subcellular localization and dynamics of the fused partner proteins. Also, GFPs serve as fluorescent tags in size-exclusion chromatography and native-PAGE, facilitating the evaluation of expression levels and quality of the expressed fusion proteins. However, the fluorescent detection of GFPs is generally incompatible with denaturing SDS-polyacrylamide gel electrophoresis (PAGE), where the samples are heat-denatured before loading. Accordingly, detecting GFP-fused proteins after SDS-PAGE usually relies on western blotting with anti-GFP antibodies. To enable in-gel fluorescence detection of SDS-PAGE-separated GFPs, some protocols employ mild denaturing conditions to keep the GFPs intact. However, such mild denaturation sometimes results in partial denaturation of the proteins and irregular electrophoretic mobility that is not proportional to their molecular weights. Here, we demonstrate that the fully denatured GFPs can be refolded within the gel by cyclodextrin-mediated removal of SDS in the presence of 20 % methanol, enabling the in-gel fluorescence detection of the GFP-fused proteins. The protocol is compatible with subsequent total protein staining and western blotting. Although future studies are needed to clarify the scope and generality, the technique developed here would provide a simple, time- and cost-effective alternative to the immunodetection of GFPs.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115861"},"PeriodicalIF":2.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of radioligand binding assays for REV-ERBα and REV-ERBβ rev - erba和rev - erbb β放射配体结合试验的建立。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-04-04 DOI: 10.1016/j.ab.2025.115858

Thomas Koelblen, Elise P. Burris, Thomas P. Burris

{"title":"Development of radioligand binding assays for REV-ERBα and REV-ERBβ","authors":"Thomas Koelblen, Elise P. Burris, Thomas P. Burris","doi":"10.1016/j.ab.2025.115858","DOIUrl":"10.1016/j.ab.2025.115858","url":null,"abstract":"<div><div>REV-ERBα and REV-ERBβ are atypical nuclear receptors that function as ligand-dependent repressors of transcription. They play critical roles in the regulation of the circadian rhythm, inflammation, and metabolism. The natural ligand for the REV-ERBs is heme, and synthetic ligands (both agonists and antagonists) have been designed and utilized to probe the potential clinical utility of targeting REV-ERBs for drug development. Although biochemical assays that can detect REV-ERB ligands have been developed based on protein-protein interactions, no classical fluorescent- or radioligand-binding assay has yet been developed that directly detects ligand binding. Here, we describe the development of the first radioligand binding assay (RLBA) using scintillation proximity assay (SPA) technology for both REV-ERBα and REV-ERBβ using labeled STL1267, a potent REV-ERBα/β agonist we recently described. <sup>3</sup>H-STL1267 displayed saturable binding to the ligand binding domains of both REV-ERBα and REV-ERBβ with equilibrium dissociation constants (K<sub>d</sub>s) of 392 nM and 202 nM, respectively. In competition radioligand binding assays, we used unlabeled STL1267 or the well-characterized first-generation REV-ERB agonist SR9009 as competitors to <sup>3</sup>H-STL1267 binding. STL1267 displayed K<sub>i</sub>s for REV-ERBα and REV-ERBβ of 253 ± 30 nM and 98 ± 14 nM, respectively. As expected, SR9009 displayed considerably lower potency than STL1267, with a K<sub>i</sub> of 692 ± 209 nM for REV-ERBα and 2546 ± 127 nM for REV-ERBβ. Although developing an RLBA has been challenging due to the lack of high-affinity ligands that can be used as probes, our results demonstrate the feasibility of such an assay for both receptors, providing a robust assay with utility for ligand/drug discovery.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115858"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative assessment of four virus neutralization assays for detection of SARS-CoV-2 neutralizing antibodies 四种病毒中和法检测SARS-CoV-2中和抗体的比较评价。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-04-03 DOI: 10.1016/j.ab.2025.115860

Faezeh Maghsood , Navid Dashti , Tannaz Bahadori , Forough Golsaz-Shirazi , Christiane Moog , Mohammad Mehdi Amiri , Fazel Shokri

{"title":"Comparative assessment of four virus neutralization assays for detection of SARS-CoV-2 neutralizing antibodies","authors":"Faezeh Maghsood , Navid Dashti , Tannaz Bahadori , Forough Golsaz-Shirazi , Christiane Moog , Mohammad Mehdi Amiri , Fazel Shokri","doi":"10.1016/j.ab.2025.115860","DOIUrl":"10.1016/j.ab.2025.115860","url":null,"abstract":"<div><div>Neutralizing antibodies (NAbs) targeting receptor-binding domain (RBD) or spike of SARS-CoV-2 play an important role in blocking virus entry to the host cells and detecting their levels is critical for the assessment of humoral protective immune response following vaccination or recovery from SARS-CoV-2 infection. Here, we compared the performance of four virus neutralization tests to measure neutralizing antibodies in various sample types. We analyzed 25 serum samples obtained from mice or rabbits immunized with different vaccine platforms, and also 11 mouse anti-RBD monoclonal antibodies (MAbs) using surrogate virus neutralization test (SVNT), pseudovirus neutralization test (PVNT), conventional virus neutralization test (CVNT), and one-step or two-step inhibition flowcytometry virus neutralization test (IFVNT). All four VNTs showed significant correlations with each other, though PVNT and CVNT displayed significantly lower limit of detection (LoD) compared to the other two assays. In conclusion, our findings indicate that all four VNT assays give valid and accurate results and could be employed to determine the level of SARS-CoV-2 neutralizing monoclonal and polyclonal antibodies.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115860"},"PeriodicalIF":2.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A predictive model for MGMT promoter methylation status in glioblastoma based on terahertz spectral data 基于太赫兹光谱数据的胶质母细胞瘤MGMT启动子甲基化状态的预测模型

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-29 DOI: 10.1016/j.ab.2025.115850

Minghui Du , Xianhao Wu , Zhiyan Sun , Rui Tao , Peiyuan Sun , Shaowen Zheng , Zhaohui Zhang , Tianyao Zhang , Xiaoyan Zhao , Pei Yang

引用次数: 0

Development of Pt/Au/PPy-COOH multisegmental nanowires modified label-free impedimetric immunosensor to determine mucin 1 (MUC1) Pt/Au/ py - cooh多段纳米线修饰无标记阻抗免疫传感器mucin 1的研制

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-28 DOI: 10.1016/j.ab.2025.115857

Baha Öndeş, Deniz Aktaş Uygun

{"title":"Development of Pt/Au/PPy-COOH multisegmental nanowires modified label-free impedimetric immunosensor to determine mucin 1 (MUC1)","authors":"Baha Öndeş, Deniz Aktaş Uygun","doi":"10.1016/j.ab.2025.115857","DOIUrl":"10.1016/j.ab.2025.115857","url":null,"abstract":"<div><div>In this study, a label-free nanowire-based impedimetric immunosensor was developed for the purpose of determining cancer biomarkers mucin 1 (MUC1). Nanowires were selected for sensor modification due to their high catalytic properties and high enzyme loading capacity. The synthesis, characterization, and application of Pt/Au/PPy-COOH nanowires to modify SPE electrodes were conducted. The nanowire-based immunosensors developed as a result of this research demonstrated a broad linear working range for MUC1 (20–3000 fg/mL), a low LOD value (0.244 fg/mL), and a low LOQ value (0.815 fg/mL). The nanowire-based immunosensor exhibited several notable characteristics. Firstly, it demonstrated excellent reproducibility, selectivity, and long-term stability. Furthermore, it demonstrated notable regenerative capabilities. It is noteworthy that the sensor exhibited the capability to detect MUC1 in commercial human serum samples, even in the presence of interfering agents. The affordability, simplicity, and expeditious analysis of nanowire-based immunosensors render them more appealing than alternative commercial kits. Consequently, these sensors hold considerable promise for clinical applications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115857"},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step cascade method via glucose oxidase-copper ion complex for detecting glucose using a portable device 通过葡萄糖氧化酶-铜离子络合物的一步级联方法，用于使用便携式装置检测葡萄糖

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-28 DOI: 10.1016/j.ab.2025.115856

Qingxi Wu, Hongxuan Zhang, Li Fu, Li Jia

{"title":"One-step cascade method via glucose oxidase-copper ion complex for detecting glucose using a portable device","authors":"Qingxi Wu, Hongxuan Zhang, Li Fu, Li Jia","doi":"10.1016/j.ab.2025.115856","DOIUrl":"10.1016/j.ab.2025.115856","url":null,"abstract":"<div><div>In this study, a one-step cascade fluorescence method was developed for the detection of glucose in honey, based on the glucose oxidase-copper ion complexes (GOx@Cu<sup>2+</sup>). These complexes exhibit dual enzymatic activities—glucose oxidase and peroxidase-like activities—which enable them to catalyze a cascade reaction. This reaction involves the oxidation of glucose and <em>o</em>-phenylenediamine (OPD), leading to the formation of 2,3-diaminophenazine (oxOPD), a compound with fluorescent properties. The proposed method overcomes the challenges of pH mismatch between enzymes and streamlines the testing process, eliminating the need for nanomaterial preparation and reducing the detection time to just 20 min. The feasibility of the method was validated by analyzing three honey samples, achieving recoveries between 96.4 % and 106 %, with relative standard deviations of less than 1.9 %. The selectivity and accuracy of the method were verified by capillary electrophoresis in three honey samples. Moreover, a self-designed portable device was introduced to enable on-site glucose detection.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115856"},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

scCorrect: Cross-modality label transfer from scRNA-seq to scATAC-seq using domain adaptation scCorrect：使用域适应从scRNA-seq到scacc -seq的跨模态标签转移。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-27 DOI: 10.1016/j.ab.2025.115847

Yan Liu , Wenyi Pei , Li Chen , Yu Xia , He Yan , Xiaohua Hu

{"title":"scCorrect: Cross-modality label transfer from scRNA-seq to scATAC-seq using domain adaptation","authors":"Yan Liu , Wenyi Pei , Li Chen , Yu Xia , He Yan , Xiaohua Hu","doi":"10.1016/j.ab.2025.115847","DOIUrl":"10.1016/j.ab.2025.115847","url":null,"abstract":"<div><div>Cell type annotation in single-cell chromatin accessibility sequencing (scATAC-seq) is crucial for enabling researchers to identify subpopulations of cells associated with specific diseases, elucidate gene regulatory networks, and discover markers indicative of disease states. The prevailing approach for cell type annotation in single-cell research involves transferring well-delineated cell types from single-cell RNA sequencing (scRNA-seq) data to scATAC-seq data using a label propagation algorithm. However, the inherent modal discrepancies (i.e.biological interpretation) between scRNA-seq and scATAC-seq data, coupled with the intrinsic sparsity and high dimensionality of scATAC-seq data, pose significant challenges to the efficacy of this strategy. To address these challenges, we introduce a novel neural network framework, scCorrect, which operates in two distinct phases. In the first phase, scCorrect aligns the scRNA-seq and scATAC-seq datasets, generating initial annotation results. The second phase involves training a corrective network specifically designed to amend any erroneous annotations produced during the first phase. Empirical tests across multiple datasets have demonstrated that scCorrect consistently achieves superior recognition accuracy, underscoring its significant potential to enhance disease-related research in humans.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115847"},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-stabilized copper oxide nanoparticles: A novel colorimetric approach for ascorbic acid sensing 壳聚糖稳定的氧化铜纳米颗粒：抗坏血酸传感的新型比色法

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-27 DOI: 10.1016/j.ab.2025.115855

Nazish Kanwal , Mansoor Khan , Saeed Ahmad Khan , Ahmed Bari , Essam A. Ali , Wei Sun , Tanzila Rehman , Umar Nishan , Amir Badshah

{"title":"Chitosan-stabilized copper oxide nanoparticles: A novel colorimetric approach for ascorbic acid sensing","authors":"Nazish Kanwal , Mansoor Khan , Saeed Ahmad Khan , Ahmed Bari , Essam A. Ali , Wei Sun , Tanzila Rehman , Umar Nishan , Amir Badshah","doi":"10.1016/j.ab.2025.115855","DOIUrl":"10.1016/j.ab.2025.115855","url":null,"abstract":"<div><div>Ascorbic acid is implicated in various diseases such as scurvy, oxidative stress, cardiovascular diseases, etc. Herein, for the first time, a simple and efficient strategy was used to synthesize cross-linked chitosan-stabilized copper oxide nanoparticles (CuO@C-CS) as a non-toxic and biodegradable-based approach. Various spectroscopic techniques, including FTIR, XRD, SEM, EDX, TGA, and elemental mapping, confirmed the synthesis of the material. The synthesized nanozyme (CuO@C-CS) was used as a peroxidase mimic for the detection of ascorbic acid, through the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with the assistance of hydrogen peroxide. The synthesized mimic enzyme transforms colorless TMB into oxTMB. The sensing of ascorbic acid was achieved through the peroxidase-like inhibitory activity of the mimic enzyme along with the reduction of oxTMB. The sensor system was fine-tuned, and it showed a limit of detection, a limit of quantification, a linear range, and regression coefficient values of 0.24 μM, 0.80 μM, 1–96 μM, and 0.999, respectively. The fabricated sensor was very selective in the presence of various potential interferents. The proposed sensor was successfully applied to commercially available orange juices for the qualitative and quantitative determination of ascorbic acid. The sensor can be used for the determination of ascorbic acid in biomedical and food samples.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115855"},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel network construction algorithm for the study of similarity and differential mechanisms between different clinical treatments: From key metabolites to the related genes for personalized therapy of breast cancer 新型网络构建算法研究不同临床治疗之间的相似性和差异性机制：从关键代谢物到乳腺癌个体化治疗的相关基因

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-26 DOI: 10.1016/j.ab.2025.115852

Xin Huang , Hanjun Cai , Xinyu He , Yong Wang , Yang Zhou

{"title":"Novel network construction algorithm for the study of similarity and differential mechanisms between different clinical treatments: From key metabolites to the related genes for personalized therapy of breast cancer","authors":"Xin Huang , Hanjun Cai , Xinyu He , Yong Wang , Yang Zhou","doi":"10.1016/j.ab.2025.115852","DOIUrl":"10.1016/j.ab.2025.115852","url":null,"abstract":"<div><div>Breast cancer (BC) is the most common diagnosed cancer in the female population. Different near-infrared (NIR)-based technologies have been generally applied for BC clinical treatment. In this study, a novel network construction algorithm based on molecular vertical relationship (NCVR) was proposed to identify key network signals for clinical personalized treatment. In NCVR, the molecular vertical relationship that can be characterized in simple terms was proposed for network construction, thereby facilitating to better advance clinical decision making. To effectively measure the discriminative ability of molecular vertical relationship between different physiological and pathological stages, the joint probability mass function was constructed using sample frequency which can reduce the influence of sample variability caused by individual differences and the probability of over fitting caused by the high complexity of molecular expression data. NCVR was successfully employed to analyze the similarities and differences of living organisms treated by different treatment patterns (i.e., NIR and apoferritin-conjugated cypate (Cy@AFT) + NIR) on BC. The results of similarity analysis indicated that the reprogramming of cellular lipid and energy metabolism may be responsible for the BC cell death induced by treatments. Experimental results of difference analysis suggested that the disruptions in cholesterol metabolism, ferroptosis and severe lipid metabolism imbalances etc. contribute to the enhanced effectiveness of BC treatment with Cy@AFT + NIR. Then, analysis results of genes related to the selected key metabolites further provided deep insights into pathological alterations associated with BC development and illustrated why the performance of Cy@AFT + NIR therapy is better than that of NIR therapy.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115852"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0