Analytical biochemistry最新文献_第6页

Investigating bottom phase extraction from aqueous two-phase systems for detecting bacteria using the lateral-flow immunoassay 研究利用侧流免疫测定从两相水溶液系统中进行底相萃取以检测细菌。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-31 DOI: 10.1016/j.ab.2024.115634

Audrey P. Luu , Shreedevi S. Rao , Humza Y. Malik, Robin B. Shi, Adam A. Toubian, Daniel T. Kamei

{"title":"Investigating bottom phase extraction from aqueous two-phase systems for detecting bacteria using the lateral-flow immunoassay","authors":"Audrey P. Luu , Shreedevi S. Rao , Humza Y. Malik, Robin B. Shi, Adam A. Toubian, Daniel T. Kamei","doi":"10.1016/j.ab.2024.115634","DOIUrl":"10.1016/j.ab.2024.115634","url":null,"abstract":"<div><p>Lateral-flow immunoassays (LFAs) can be used to diagnose urinary tract infections caused by <em>Escherichia coli</em> (<em>E. coli</em>) at the point of care. Unfortunately, urine samples containing dilute concentrations of <em>E. coli</em> can yield false negative results on LFAs. Our laboratory was first to implement aqueous two-phase systems (ATPSs) to preconcentrate samples into smaller volumes prior to their application on LFAs. This is achieved by manipulating the ratio of the volume of the top phase to that of the bottom phase (volume ratio; VR) and concentrating biomarkers in the bottom phase which, when applied to LFAs in fixed volumes, leads to corresponding improvements in sensitivity. This work is the first demonstration that the same LOD can be achieved irrespective of the VR when the entire bottom phase is added to LFAs. A custom 3D-printed device was also developed to decrease liquid handling steps. Across different VRs expected from patient urine variability, this diagnostic workflow successfully detected <em>E. coli</em> concentrations down to 2 × 10<sup>5</sup> colony-forming units (cfu) mL<sup>−1</sup> in synthetic urine, demonstrating consistent 10-fold improvements in sensitivity compared to trials conducted without ATPS preconcentration. This method successfully addresses the variability of patient samples while remaining easy to use at the point of care.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical considerations for characterization of generic peptide product: A regulatory insight 仿制肽产品特征描述的分析考虑因素：监管视角。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-30 DOI: 10.1016/j.ab.2024.115633

Akhilesh Kumar Kuril , K. Saravanan , Praveen Kumar Subbappa

{"title":"Analytical considerations for characterization of generic peptide product: A regulatory insight","authors":"Akhilesh Kumar Kuril , K. Saravanan , Praveen Kumar Subbappa","doi":"10.1016/j.ab.2024.115633","DOIUrl":"10.1016/j.ab.2024.115633","url":null,"abstract":"<div><p>The Peptide therapeutics market was evaluated to be around USD 45.67 BN in 2023 and is projected to witness massive growth at a CAGR of around 5.63 % from 2024 to 2032 (USD 80.4 BN). Generic peptides are expected to reach USD 27.1 billion by 2032 after the patent monopoly of the pioneer peptides expires, and generic peptides become accessible. The generic manufacturers are venturing into peptide-based therapeutics for the aforementioned reasons. There is an abundance of material accessible regarding the characterization of peptides, which can be quite confusing for researchers. The FDA believes that an ANDA applicant may now demonstrate that the active component in a proposed generic synthetic peptide drug product is the “same” as the active ingredient in a peptide of rDNA origin that has previously been approved. To ensure the efficacy, safety, and quality of peptide therapies during development, regulatory bodies demand comprehensive characterization utilizing several orthogonal methodologies. This article elaborates the peptide characterization by segmenting into different segments as per the critical quality attribute from identification of the peptide to the physicochemical property of the peptide therapeutics which will be required to demonstrate the sameness with reference product based on the size of the peptide chain and molecular weight of the peptides. Article insights briefly on each individual technique and the orthogonal techniques for each test were explained. The impurities requirements in the generic peptides as per the regulatory requirement were also discussed.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breaking barriers in pharmaceutical analysis: Streamlined UV spectrometric quantification and stability profiling of haloperidol and methylparaben in liquid formulations 打破药物分析中的障碍：简化液体制剂中氟哌啶醇和苯甲酸甲酯的紫外光谱定量和稳定性分析。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-30 DOI: 10.1016/j.ab.2024.115632

Khadidja Djilali , Rachida Maachi , Zohra Ait Mesbah , Nourddine Nasrallah , Nabil Touzout , Hichem Tahraoui , Jie Zhang , Abdeltif Amrane

{"title":"Breaking barriers in pharmaceutical analysis: Streamlined UV spectrometric quantification and stability profiling of haloperidol and methylparaben in liquid formulations","authors":"Khadidja Djilali , Rachida Maachi , Zohra Ait Mesbah , Nourddine Nasrallah , Nabil Touzout , Hichem Tahraoui , Jie Zhang , Abdeltif Amrane","doi":"10.1016/j.ab.2024.115632","DOIUrl":"10.1016/j.ab.2024.115632","url":null,"abstract":"<div><p>This study aims to quantify haloperidol and methylparaben in a liquid pharmaceutical formulation (2 mg/ml) using UV spectrometry and the simultaneous equations method. Additionally, we explored the stability of haloperidol under various stress conditions. The UV analysis revealed maximum absorption peaks at 248 nm for haloperidol and 256 nm for methylparaben, using a 1 % (v/v) lactic acid solution as the solvent. Method validation, conducted according to ICH guidelines, affirmed the method's reliability, showing excellent results in terms of linearity, precision, accuracy, and sensitivity. The method allows direct application to finished products, enabling simultaneous quantification without extractions. Its simplicity, speed, and cost-effectiveness make it ideal for routine controls in pharmaceutical industry haloperidol solution analyses. The method extends to monitoring forced degradation, indicating photolytic and hydrolytic degradation under acidic and basic conditions, while affirming thermal and oxidative stability. This proposed UV spectrometric method serves as a compelling alternative to pharmacopeia-recommended techniques, simplifying simultaneous determination of the active ingredient and preservative. This streamlines analysis, reducing time and costs. Additionally, it proves valuable in small industries lacking sophisticated instrumentation, offering insights into active ingredient behavior during forced degradation.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cPGA hydrolase assay of DJ-1 in crude cell lysates: Implications for sensing of oxidative stress 粗细胞裂解物中 DJ-1 的 cPGA水解酶测定：感知氧化应激的意义

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-30 DOI: 10.1016/j.ab.2024.115631

Adilet Bekmagambetov , Evelina Shkraba , Adilkhan Yeskendir , Aizhan Akhmadi , Darkhan Utepbergenov

{"title":"cPGA hydrolase assay of DJ-1 in crude cell lysates: Implications for sensing of oxidative stress","authors":"Adilet Bekmagambetov , Evelina Shkraba , Adilkhan Yeskendir , Aizhan Akhmadi , Darkhan Utepbergenov","doi":"10.1016/j.ab.2024.115631","DOIUrl":"10.1016/j.ab.2024.115631","url":null,"abstract":"<div><p>Cyclic 3-phosphosphoglyceric anhydride (cPGA), a side product of glycolysis, acylates cellular amines and thiols to form amides and thioesters, respectively. Since these acylation reactions are harmful, organisms rely on a protein, known as DJ-1 in humans, to inactivate cPGA. Inactivation of cPGA likely plays a significant role in cytoprotection by DJ-1, but further progress in this direction is hampered by the lack of quantitative assays to measure the cPGA hydrolase activity of DJ-1 in biological samples. Here we report an optimized procedure for preparation of cPGA which is then used as a substrate to quantify enzymatic activity of DJ-1. The end-point assay for cPGA hydrolase uses dilute cell lysates to hydrolyze cPGA for 0.5–3.5 min followed by conversion of the remaining cPGA into thioester for spectrophotometric quantitation. We illustrate the utility of this assay by showing that higher levels of cPGA hydrolase activity result in better protection from acylation by cPGA. Moreover, the decrease of cPGA hydrolase activity due to oxidation of the catalytic cysteine of DJ-1 under oxidative stress and its subsequent recovery can be monitored using the assay. This relatively simple assay allows functional characterization of DJ-1 in biological samples through quantitative assessment of its cPGA hydrolase activity.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying circRNA-disease association based on relational graph attention network and hypergraph attention network 基于关系图注意力网络和超图注意力网络识别 circRNA 与疾病的关联性

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-26 DOI: 10.1016/j.ab.2024.115628

PengLi Lu, Jinkai Wu, Wenqi Zhang

{"title":"Identifying circRNA-disease association based on relational graph attention network and hypergraph attention network","authors":"PengLi Lu, Jinkai Wu, Wenqi Zhang","doi":"10.1016/j.ab.2024.115628","DOIUrl":"10.1016/j.ab.2024.115628","url":null,"abstract":"<div><p>In recent years, with the in-depth study of circRNA, scholars have begun to discover a synergistic relationship between circRNA and microorganisms. Traditional wet lab experiments in biology require expensive financial, material, and human resources to investigate the relationship between circRNA and diseases. Therefore, we propose a new predictive model for inferring the association between circRNA and diseases, called HAGACDA. Specifically, we first aggregate the unique features of circRNA and diseases themselves through singular value decomposition, Pearson similarity, and the biological information characteristics of circRNA and diseases. Utilizing the competitive relationships between miRNA and other microorganisms, we construct a circRNA-miRNA-disease multi-source heterogeneous network. Subsequently, we use a relational graph attention network to aggregate features based on the structural connections between different nodes. To address the inherent limitations in capturing high-order patterns in edge sets, we integrate a hypergraph attention network to extract features of circRNA and diseases. Finally, association prediction scores for node pairs are obtained through a multilayer perceptron. We conducted a comprehensive analysis of the model, including comparative experiments and case studies. Experimental results demonstrate that our model accurately predicts the association between circRNA and diseases.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Xiaochaihu Decoction's fever-relieving mechanism via network pharmacology, molecular docking, dynamics simulation, and experiments 通过网络药理学、分子对接、动力学模拟和实验研究小柴胡汤的解热机制

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-26 DOI: 10.1016/j.ab.2024.115629

Hong Lei, Hongbing Su, Ling Cao, Xiaoying Zhou, Yumeng Liu, Ying Li, Xiaoxue Song, Yanhong Wang, Qingxia Guan

{"title":"Investigating Xiaochaihu Decoction's fever-relieving mechanism via network pharmacology, molecular docking, dynamics simulation, and experiments","authors":"Hong Lei, Hongbing Su, Ling Cao, Xiaoying Zhou, Yumeng Liu, Ying Li, Xiaoxue Song, Yanhong Wang, Qingxia Guan","doi":"10.1016/j.ab.2024.115629","DOIUrl":"10.1016/j.ab.2024.115629","url":null,"abstract":"<div><p>Xiaochaihu Decoction（XCHD）is a classic prescription for the treatment of fever, but the mechanism is not clear. In this study, We elucidated the mechanism of action through network pharmacology and molecular docking. A rat fever model was established to verify the prediction results of network pharmacology. The analysis revealed that 120 intersection targets existed between XCHD and fever. The TP53, STAT3, RELA, MAPK1, AKT1, TNF and MAPK14 as potential core targets of XCHD in fever treatment. GO and KEGG pathway enrichment analyses indicated that XCHD may act through pathways such as the AGE-RAGE signaling pathway in diabetic complications, TNF signaling pathway, IL-17 signaling pathway. Molecular docking results demonstrated that quercetin, kaempferol, β-sitosterol, stigmasterol and baicalein exhibited strong binding activity to key targets. Animal experiments showed that XCHD significantly reduced body temperature and levels of IL-1β, IL-6, TNF-α, NO, PGE2, and cAMP in rats with fever. Importantly, no significant difference was observed between the XCHD self-emulsifying nano phase plus suspension phase and XCHD group. XCHD exerts its therapeutic effects on fever through a multi-ingredient, multi-target, and multi-pathway approach.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of an enzyme-linked immunosorbent assay for the quantification of a recombinant humanized anti-IL-4Rα monoclonal antibody CM310 in serum and its application to pharmacokinetic study in Sprague-Dawley Rats 开发和验证用于血清中重组人源化抗IL-4Rα单克隆抗体CM310定量的酶联免疫吸附测定及其在Sprague-Dawley大鼠药代动力学研究中的应用。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-25 DOI: 10.1016/j.ab.2024.115623

Yimeng Hao , Libo Zhang , Qinghe Meng , Qian Jia , Jing Ma , Xuemei Zhang

{"title":"Development and validation of an enzyme-linked immunosorbent assay for the quantification of a recombinant humanized anti-IL-4Rα monoclonal antibody CM310 in serum and its application to pharmacokinetic study in Sprague-Dawley Rats","authors":"Yimeng Hao , Libo Zhang , Qinghe Meng , Qian Jia , Jing Ma , Xuemei Zhang","doi":"10.1016/j.ab.2024.115623","DOIUrl":"10.1016/j.ab.2024.115623","url":null,"abstract":"<div><p>CM310 is a recombinant humanized monoclonal antibody targeting Interleukin (IL)-4 receptor alpha (IL-4Rα). IL-4Rα blockade prevents IL-4 and IL-13 from binding to their receptor, thereby inhibiting downstream signaling pathways that drive Type 2 helper T-cell (Th2) inflammation. CM310 holds potential for treating Th2-related inflammatory diseases, such as asthma, atopic dermatitis and chronic sinusitis with nasal polyposis. In this study, a direct enzyme-linked immunosorbent assay (ELISA) was developed to measure the concentrations of CM310 in rat serum. Seven calibration standards (ranging from 25 to 1600 ng/mL) and three quality controls (70, 500 and 1250 ng/mL) were defined. The limit of detection (LOD), lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ) were 13, 25 and 1600 ng/mL, respectively. The method exhibited excellent precision and accuracy and successfully applied to <em>in vitro</em> serum stability and pharmacokinetic (PK) studies. In conclusion, we have developed and validated a highly sensitive and selective method for measuring CM310 in Sprague-Dawley rats. The development and validation ELISA method met the acceptable criteria, which suggested that these can be applied to quantify CM310, as well as in PK studies.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry 利用毛细管电泳和电感耦合等离子体质谱法测定蓝藻 Nostoc sp.

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-24 DOI: 10.1016/j.ab.2024.115630

Natália Melicherová , Tomáš Vaculovič , Radka Kočí , Martin Trtílek , Jana Lavická , František Foret

引用次数: 0

LC-MS characterization of N/O-glycans of α- and β-subunits of chicken ovomucin separated by SDS-PAGE 用 SDS-PAGE 分离鸡卵母细胞蛋白 α 和 β 亚基的 N/O-Glycans 的 LC-MS 特征。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-20 DOI: 10.1016/j.ab.2024.115625

Cheng Li, Qinghui Chen, Jinqiao Rong, Houde He, Yu Lu, Yuxia Liu, Zhongfu Wang

{"title":"LC-MS characterization of N/O-glycans of α- and β-subunits of chicken ovomucin separated by SDS-PAGE","authors":"Cheng Li, Qinghui Chen, Jinqiao Rong, Houde He, Yu Lu, Yuxia Liu, Zhongfu Wang","doi":"10.1016/j.ab.2024.115625","DOIUrl":"10.1016/j.ab.2024.115625","url":null,"abstract":"<div><p>As the main active glycoprotein of egg white, the biological functions of chicken ovomucin α- and β-subunit are closely related to the structure of glycans. However, the exact composition and structure of the subunit glycans are still unknown. We obtained highly pure chicken ovomucin α-subunit and β-subunit protein bands by the strategy combined with two-step isoelectric precipitation and SDS-PAGE gel electrophoresis. The ammonia-catalyzed one-pot procedure was then used to release and capture α-and β-subunit protein glycans with 1-phenyl- 3-Methyl-5-pyrazolone (PMP). The <em>N</em>/<em>O</em>-glycans of bis-PMP derivatives were purified and analyzed by LC-MS. More importantly, an effective dual modification was performed to accurately quantify neutral and sialylated <em>O</em>-glycans through methylamidation of sialic acid residues and simultaneously through carbonyl condensation reactions of reducing ends with PMP. We first showed that the α-subunit protein has only <em>N</em>-glycosylation modification, and the β-subunit only <em>O</em>-glycosylation, a total of 22 <em>N</em>-glycans and 20 <em>O</em>-glycans were identified in the α- and β-subunit, respectively. In addition, the complex <em>N</em>-glycan (47 %) and the sialylated <em>O</em>-glycan (77 %) are each major types of the above subunits. Such findings in this study provide a basis for studying the functional and biological activities of chicken ovomucin glycans.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application and performance enhancement of FAIMS spectral data for deep learning analysis using generative adversarial network reinforcement 利用生成式对抗网络强化 FAIMS 光谱数据在深度学习分析中的应用和性能提升。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-07-20 DOI: 10.1016/j.ab.2024.115627

Ruilong Zhang, Xiaoxia Du, Hua Li

{"title":"Application and performance enhancement of FAIMS spectral data for deep learning analysis using generative adversarial network reinforcement","authors":"Ruilong Zhang, Xiaoxia Du, Hua Li","doi":"10.1016/j.ab.2024.115627","DOIUrl":"10.1016/j.ab.2024.115627","url":null,"abstract":"<div><p>When using High-field asymmetric ion mobility spectrometry (FAIMS) to process complex mixtures for deep learning analysis, there is a problem of poor recognition performance due to the lack of high-quality data and low sample diversity. In this paper, a Generative Adversarial Network (GAN) method is introduced to simulate and generate highly realistic and diverse spectral for expanding the dataset using real mixture spectral data of 15 classes collected by FAIMS. The mixed datasets were put into VGG and ResNeXt for testing respectively, and the experimental results proved that the best recognition effect was achieved when the ratio of real data to generated data was 1:4: where accuracy improved by 24.19 % and 6.43 %; precision improved by 23.71 % and 6.97 %; recall improved by 21.08 % and 7.09 %; and F1-score improved by 24.50 % and 8.23 %. The above results strongly demonstrate that GAN can effectively expand the data volume and increase the sample diversity without increasing the additional experimental cost, which significantly enhances the experimental effect of FAIMS spectral for the analysis of complex mixtures.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0