Analytical biochemistry最新文献_第6页

Next-generation electrochemical biosensors for acrylamide: Progress, challenges, and opportunities

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115798

Shatrughan Aafria, Minakshi Sharma

{"title":"Next-generation electrochemical biosensors for acrylamide: Progress, challenges, and opportunities","authors":"Shatrughan Aafria, Minakshi Sharma","doi":"10.1016/j.ab.2025.115798","DOIUrl":"10.1016/j.ab.2025.115798","url":null,"abstract":"<div><div>Acrylamide is a hazardous substance present in heat-processed food products and industrial wastewater. It is carcinogenic and neurotoxic and therefore emphasises the importance of monitoring its levels and the need for sensitive and accurate detection techniques. Electrochemical biosensing has emerged as a potential analytical method for detecting acrylamide. This article provides a comprehensive overview of the most recent developments in electrochemical biosensing methods, including amperometric, potentiometric, and impedimetric biosensors for acrylamide detection. The creation and use of novel biorecognition components, such as enzymes, antibodies, and molecularly imprinted polymers that enhance the sensitivity and specificity of acrylamide monitoring, are given special attention. Incorporating nanomaterials such as carbon-based nanomaterials and metallic nanoparticles was investigated for its potential to improve the sensors' electrochemical characteristics and overall efficacy. The potential of electrochemical biosensors for acrylamide detection is further illustrated, showcasing their effectiveness in a range of matrices in different food products. This review aims to inform researchers about the latest technological developments, trends, and future directions in electrochemical biosensing for acrylamide detection. The study highlights the significance of ongoing research and cooperation in creating efficient biosensing systems to protect public health and the environment by thoroughly examining current technology and pointing out areas for improvement.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115798"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total flavonoid content revised: An overview of past, present, and future determinations in phytochemical analysis

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115794

Alexandru Nicolescu , Claudiu Ioan Bunea , Andrei Mocan

{"title":"Total flavonoid content revised: An overview of past, present, and future determinations in phytochemical analysis","authors":"Alexandru Nicolescu , Claudiu Ioan Bunea , Andrei Mocan","doi":"10.1016/j.ab.2025.115794","DOIUrl":"10.1016/j.ab.2025.115794","url":null,"abstract":"<div><div>Flavonoids represent an important research topic in the analytical chemistry of secondary plant metabolites. During habitual laboratory determinations, preliminary quantitative analysis is often associated with <em>in vitro</em> colorimetric assessment. Total flavonoid content (TFC) is used as screening method with high relevance in the chemical analysis of plants and derived products, being typically applied before HPLC-MS phytochemical profiling. Its importance stems from affordability, simplicity, rapidity, and low cost. The AlCl<sub>3</sub> assay, with or without NaNO<sub>2</sub> addition, is the most used method in the present, although less frequently used methods (using 2,4-dinitrophenylhydrazine, dimethylamino-cinnamaldehyde, or diethylene glycol) show potential for complementary and specific determinations. Given the prevalence of research papers focusing on a single method for “total flavonoid” determination, we identified the need for an objective and critical comparison of existing methodologies. Moreover, a special notice is dedicated to the past and the future of <em>in vitro</em> TFC determinations, in the context of recent advances in flavonoid research. The focal point of this review is to serve as a basis for laboratory protocol reorganization regarding TFC determination, as a powerful tool before mass spectrometry, as well as to present a potential complementary analysis protocol applicable to biological samples. Among the methods found in the literature, SBC was the only assay providing accurate determinations of TFC.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115794"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopores for DNA and biomolecule analysis: Diagnostic, genomic insights, applications in energy conversion and catalysis

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115791

Pradakshina Sharma , Tasmiya Sana , Shaheen Khatoon , Ubiad Mushtaq Naikoo , Mosina , Nitesh Malhotra , Md Saquib Hasnain , Amit Kumar Nayak , Jagriti Narang

{"title":"Nanopores for DNA and biomolecule analysis: Diagnostic, genomic insights, applications in energy conversion and catalysis","authors":"Pradakshina Sharma , Tasmiya Sana , Shaheen Khatoon , Ubiad Mushtaq Naikoo , Mosina , Nitesh Malhotra , Md Saquib Hasnain , Amit Kumar Nayak , Jagriti Narang","doi":"10.1016/j.ab.2025.115791","DOIUrl":"10.1016/j.ab.2025.115791","url":null,"abstract":"<div><div>Recently, nanopores have emerged as highly significant structures with broad applications in diverse scientific and technological fields. They can naturally occur in biological membranes or be artificially fabricated using advanced techniques. Recent advances in nanopore technology have revolutionized genomics by offering previously unheard-of capacities for deoxyribo nucleic acid (DNA) sequencing and analysis. These tiny pores allow individual molecules to be found more easily, allowing for real-time DNA analysis and providing currently unheard-of insights into genetics and diagnostics. By tracking alterations in electrical or ionic currents as biomolecules traverse the pore, nanopores make possible the real-time recognition of other biomolecules, like proteins, nucleic acids, and small molecules, eliminating the need for labeling. This label-free detection potential holds a huge promise in medical diagnostics, genotyping, environmental monitoring, <em>etc</em>. Nanopores have significantly improved DNA sequencing technology such as increment in read length, enabling researchers to sequence entire genomic regions, accuracy can be improved and recent updates have led to a reported increase in total DNA reads, demonstrating the technology's capacity for high-throughput applications <em>via</em> trapping individual DNA strands and monitoring the variations of ionic current as each nucleotide passes across the pore. Finally, nanopore sequencing is well-known as a novel and highly flexible technique for DNA analyses, which has a huge deal of promise in clinical diagnosis and genomics research. Hence, this review article comprehensively explains nanopores for DNA analysis and other biomolecules, their synthesis, and diverse applications.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115791"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation and clinical performance of a non-commercial ELISA for SARS-CoV-2 anti-RBD IgA antibodies

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.ab.2025.115787

Sheila Chávez-Valdés , Ana K. Marichal-Rodríguez , Yahima Chacón-Quintero , Ricardo Martínez-Rosales , Nivaldo Gómez-Hernández , Lismary Ávila-Díaz , Amalia Vázquez-Arteaga , Hany González-Formental , Giselle Freyre-Corrales , Edelgis Coizeau-Rodríguez , Gerardo Guillen , Gilda Lemos-Pérez

{"title":"Validation and clinical performance of a non-commercial ELISA for SARS-CoV-2 anti-RBD IgA antibodies","authors":"Sheila Chávez-Valdés , Ana K. Marichal-Rodríguez , Yahima Chacón-Quintero , Ricardo Martínez-Rosales , Nivaldo Gómez-Hernández , Lismary Ávila-Díaz , Amalia Vázquez-Arteaga , Hany González-Formental , Giselle Freyre-Corrales , Edelgis Coizeau-Rodríguez , Gerardo Guillen , Gilda Lemos-Pérez","doi":"10.1016/j.ab.2025.115787","DOIUrl":"10.1016/j.ab.2025.115787","url":null,"abstract":"<div><div>COVID-19 is caused by SARS-CoV-2, first identified in 2019. The Cuban vaccines, Abdala and Mambisa, have demonstrated efficacy in preventing SARS-CoV-2 infection. Immunoglobulin A (IgA) are the main line of defense against pathogens invading the respiratory or digestive tract and its presence in serum can be measured to assess vaccine efficacy. ELISAs are a valuable tool for assessing vaccine immunogenicity. These tests should be validated to ensure their reliability and suitability. The objective of this study was to validate a non-commercial ELISA for the quantification of total anti-RBD IgA in serum samples to support clinical studies. This assay demonstrated high clinical specificity (97.3 %). The accuracy and precision of the assay showed an overall error of less than 20 % at all levels in QCs. Re-evaluation of samples showed a mean difference of less than 30 % in 90.2 % of cases. Anti-RBD IgA titers correlated with viral neutralization titers and percentage inhibition of RBD-ACE2 binding. This assay was found to be highly accurate and reproducible for the quantification of anti-RBD IgA, met the most stringent acceptance criteria and is fit for purpose. It is currently being used to evaluate the immunogenicity of the Abdala and Mambisa vaccines.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115787"},"PeriodicalIF":2.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the physico-chemical properties of amino acids and chemically functionalized surfaces on DIOS-MS analysis

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-30 DOI: 10.1016/j.ab.2025.115792

Antonin Lavigne , Thomas Géhin , Benoît Gilquin , Laetitia-Eiko Xerri , Marc Veillerot , Vincent Jousseaume , Yann Chevolot , Magali Phaner-Goutorbe , Christelle Yeromonahos

{"title":"Effects of the physico-chemical properties of amino acids and chemically functionalized surfaces on DIOS-MS analysis","authors":"Antonin Lavigne , Thomas Géhin , Benoît Gilquin , Laetitia-Eiko Xerri , Marc Veillerot , Vincent Jousseaume , Yann Chevolot , Magali Phaner-Goutorbe , Christelle Yeromonahos","doi":"10.1016/j.ab.2025.115792","DOIUrl":"10.1016/j.ab.2025.115792","url":null,"abstract":"<div><div>Desorption ionization on silicon mass spectrometry (DIOS-MS) allows for the detection of low molecular weight species from fluid samples. However, this method remains scarcely used for clinical diagnosis likely because of a lack of knowledge about the desorption/ionization mechanism as well as about the interplay between the surface and analyte properties which are effective in desorption/ionization, impeding the optimization of the DIOS-MS analysis.</div><div>Herein, the normalized intensity of the DIOS-MS peaks at [M+H]<sup>+</sup> of seven amino acids on four different porous silicon modified surfaces are investigated. These amino acids (arginine, phenylalanine, methionine, glutamine, leucine, cysteine and valine) have different isoelectric points, proton affinities, and octanol-water partition coefficients. The four selected surfaces were oxidized porous silicon (SiO<sub>2</sub>), the same porous silicon modified with a propyl dimethyl ethoxy silane, octadecyl dimethyl ethoxy silane or 3 amino propyl dimethyl ethoxy silane (CH<sub>3</sub>-short, CH<sub>3</sub>-long and NH<sub>3</sub><sup>+</sup>, respectively). These surfaces present different electrical charges, alkyl chain lengths, and hydrophilic/hydrophobic properties. For each surface, the intensities of the protonated molecules ([M+H]<sup>+</sup>) are discussed with respect to the electrical charge and proton affinity of the amino acids, their z-distributions inside the pores (determined by time of flight secondary ion mass spectrometry profiling), their surface interaction energies (calculated by molecular dynamics simulations), the interfacial water content and the proton availability for each surface.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115792"},"PeriodicalIF":2.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-technique approach to enlighten the role of metal coordination in calcitermin antiviral properties

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-30 DOI: 10.1016/j.ab.2025.115784

Anna Caproni , Silvia Leveraro , Klaudia Szarszoń , Chiara Nordi , Riccardo Fontana , Mattia Buratto , Peggy Marconi , Maurizio Remelli , Mariaconcetta Sicurella , Denise Bellotti

{"title":"A multi-technique approach to enlighten the role of metal coordination in calcitermin antiviral properties","authors":"Anna Caproni , Silvia Leveraro , Klaudia Szarszoń , Chiara Nordi , Riccardo Fontana , Mattia Buratto , Peggy Marconi , Maurizio Remelli , Mariaconcetta Sicurella , Denise Bellotti","doi":"10.1016/j.ab.2025.115784","DOIUrl":"10.1016/j.ab.2025.115784","url":null,"abstract":"<div><div>In this work we presented how the use of suitable electroanalytical, thermodynamic and spectroscopic methods combined with proper experimental conditions can provide comprehensive information on the interaction between metal ions and peptides in solution, as a successful strategy for studying biological systems. Our candidate peptide is calcitermin, an effective metal chelator with significant anti-<em>Candida</em> and antibacterial activity in the presence of divalent metals. While the bioinorganic chemistry of calcitermin with zinc and copper is quite well described in the literature, no data about nickel complexes are available; we therefore deepened calcitermin ability to form nickel complexes by different analytical techniques, including potentiometry, ultraviolet–visible absorption spectrophotometry, circular dichroism and high-resolution mass spectrometry. Moreover, for the first time we have investigated the antiviral activity of calcitermin and its metal complexes towards <em>Herpes simplex</em> type 1. Despite the nickel-associated slow kinetics, which requires specific experimental precautions, calcitermin forms stable complexes with this cation at different pH conditions. Both the apopeptide and its metal complexes show a random coil secondary structure, which is often characteristic of viral cellular adhesion inhibition. This research highlights that calcitermin and its metal complexes can interfere with viral infections, particularly HSV-1, most likely by altering cell membrane permeability.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115784"},"PeriodicalIF":2.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vivo microwave-aided spectrofluorimetric characterization of teneligliptin-loaded solid dispersion adsorbate using quality by design approach

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-30 DOI: 10.1016/j.ab.2025.115785

Pintu Prajapati , Princy Ardeshana , Yashwini Kansara , Pranav Shah , Veera Shakar Pulusu , Anzarul Haque , Mohd Abul Kalam , Shailesh Shah

{"title":"In-vivo microwave-aided spectrofluorimetric characterization of teneligliptin-loaded solid dispersion adsorbate using quality by design approach","authors":"Pintu Prajapati , Princy Ardeshana , Yashwini Kansara , Pranav Shah , Veera Shakar Pulusu , Anzarul Haque , Mohd Abul Kalam , Shailesh Shah","doi":"10.1016/j.ab.2025.115785","DOIUrl":"10.1016/j.ab.2025.115785","url":null,"abstract":"<div><div>This study developed a microwave-aided spectrofluorimetric method for in-vivo characterizing teneligliptin-loaded solid dispersion adsorbate (TNG-SDA) using a quality by design approach. The microwave-aided chemical reaction was applied for derivatization of non-fluorescent teneligliptin with NBD-Cl (7-chloro-4-nitrobenzoxadiazole). TNG-SDA was prepared to enhance the solubility and bioavailability of teneligliptin, a BCS (Biopharmaceutical Classification System) class II drug. The method utilized environmentally friendly solvents and employed analytical quality by design principles for optimization. Critical method parameters were identified through failure mode impact analysis and optimized using response surface modelling. The fluorescence spectra of teneligliptin exhibited linearity across the concentration range of 50–250 ng/mL, with a correlation coefficient of 0.9978. The developed method was found to be accurate, precise, robust, specific and sensitive for estimation of teneligliptin. The method was applied to evaluate in vitro dissolution, pharmacokinetics, and pharmacodynamic of TNG-SDA compared to commercial formulations. Results showed significant improvements in teneligliptin solubility and bioavailability with the developed SDA. The microwave-aided derivatized fluorescent product was characterized by mass spectrometry. The method demonstrated environmental sustainability, robustness, sensitivity, cost-effectiveness, and user-friendliness when evaluated using white analytical chemistry approaches. It provided a sensitive and green alternative to existing hyphenated techniques for nanogram-level teneligliptin detection in pharmacokinetic studies.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115785"},"PeriodicalIF":2.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disinfection by hydrogen peroxide at low concentration in air: The key role of condensation

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-29 DOI: 10.1016/j.ab.2025.115786

P. Destrez , D. Beysens

{"title":"Disinfection by hydrogen peroxide at low concentration in air: The key role of condensation","authors":"P. Destrez , D. Beysens","doi":"10.1016/j.ab.2025.115786","DOIUrl":"10.1016/j.ab.2025.115786","url":null,"abstract":"<div><div>Gazeous hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is commonly used for disinfection of room surfaces or sterilization of medical devices. Disinfection and sterilization processes are controlled by mean values measured at sterilizer chamber or room level. However, the surface phenomena (adsorption/condensation) taking place on inoculum are essential and still not well-known. In the present study, a solution of water and H<sub>2</sub>O<sub>2</sub> is sprayed in a room disinfection system (Glosair 400, ASP) for 12 min. Condensation mass, H<sub>2</sub>O<sub>2</sub> concentration, relative humidity (RH), macro zoom observations and inactivation kinetics of various microorganisms (<em>Staphyloccocus aureus</em>, <em>Pseudomonas aeruginosa Candida albicans</em>, <em>Aspergillus niger)</em> are reported. Macro-zoom observations reveal condensation and bubbling activities. Microbial inactivation is found optimal at low initial RH, corresponding to high H<sub>2</sub>O<sub>2</sub> vapor concentration and low condensed mass. H<sub>2</sub>O<sub>2</sub> concentration in the condensate is high and probably boosted by fractional condensation. In surface disinfection processes, inactivation of microorganisms occurs in presence of condensation although excessive condensation, due to high initial RH conditions or the presence of salt, decreases the microbial inactivation efficiency by dilution. While the present experimental conditions differ from those prevailing in H<sub>2</sub>O<sub>2</sub> sterilization (59 % H<sub>2</sub>O<sub>2</sub> under vacuum) or industrial disinfection processes (30 % H<sub>2</sub>O<sub>2</sub> at atmospheric pressure), they are partially transposable.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"701 ","pages":"Article 115786"},"PeriodicalIF":2.6,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in biosensors: A breakthrough in rapid and precise brucellosis detection

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-28 DOI: 10.1016/j.ab.2025.115782

Roya Rasooly Heshteli , Giti Paimard , Mahdi Adabi , Saber Esmaeili

{"title":"Advances in biosensors: A breakthrough in rapid and precise brucellosis detection","authors":"Roya Rasooly Heshteli , Giti Paimard , Mahdi Adabi , Saber Esmaeili","doi":"10.1016/j.ab.2025.115782","DOIUrl":"10.1016/j.ab.2025.115782","url":null,"abstract":"<div><div>Brucellosis, a significant zoonotic disease, poses a threat to both livestock and human health. Infections in livestock lead to abortion, infertility, and substantial economic losses in the industry. In humans, acute brucellosis can progress to a chronic condition, resulting in multisystemic infections with high morbidity and mortality rates. Additionally, the bioterrorism potential of certain <em>Brucella</em> species through aerosol transmission poses risks to laboratory workers and livestock handlers. Therefore, there is an urgent need for rapid and precise diagnosis of brucellosis in both animals and humans. Even with the availability of routine diagnostic techniques that are effective they frequently have some limitations. Biosensors, as innovative techniques, have demonstrated significant potential in detecting various pathogens with high efficiency. These biosensors can identify specific analytes, biomolecules of pathogenic bacteria, secreted antibodies against bacteria, and even the bacterial body in real time. Their high sensitivity, selectivity, and user-friendly configurations make them valuable tools for diagnostics. In this comprehensive review, beside the reviewing routine diagnostic tests for detecting brucellosis and discussing the positive and negative aspects of these methods, we explore different types of biosensors and their applications in diagnosing brucellosis. We hope to show how these advancements can result in quicker and more precise disease detection by offering a thorough evaluation of these technologies performance and contrasting it with more conventional diagnostic techniques. This improves patient outcomes by lowering the complications linked to delayed diagnosis in addition to advancing scientific knowledge of brucellosis.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"700 ","pages":"Article 115782"},"PeriodicalIF":2.6,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the diagnostic landscape: Portable aptasensors in point-of-care testing

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-01-28 DOI: 10.1016/j.ab.2025.115788

Anu Mohan, Ipsita Roy

引用次数: 0