Analytical biochemistry最新文献_第5页

A site-specific fluorogenic probe for protein disulfide isomerase A1 蛋白二硫异构酶A1的位点特异性荧光探针

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-25 DOI: 10.1016/j.ab.2025.115851

Yuli Zhuang , Danqi Hong , Wenjie Lang , Yinyan Xuan , Liquan Zhu , Jingyan Ge

{"title":"A site-specific fluorogenic probe for protein disulfide isomerase A1","authors":"Yuli Zhuang , Danqi Hong , Wenjie Lang , Yinyan Xuan , Liquan Zhu , Jingyan Ge","doi":"10.1016/j.ab.2025.115851","DOIUrl":"10.1016/j.ab.2025.115851","url":null,"abstract":"<div><h3>Background</h3><div>Protein disulfide isomerase A1 (PDIA1) is essential for catalyzing disulfide bond isomerization, ensuring proper protein folding, and maintaining cellular homeostasis. Dysregulation of PDIA1 function is implicated in various diseases, emphasizing the need for tools to study its activity dynamically and specifically. Despite this need, current methods lack the sensitivity and robustness required for reliable detection of PDIA1 activity.(57)</div></div><div><h3>Results</h3><div>We synthesized a series of vinyl sulfone-based fluorescent probes capable of covalently binding to thiol groups, triggering fluorescence activation. Among these, the probe <strong>LS</strong> exhibited outstanding performance, achieving a ∼18-fold fluorescence intensity increase upon binding to PDIA1. <strong>LS</strong> showed high specificity for PDIA1 by selectively targeting the cysteine residue at position 397 in its active site. The probe demonstrated rapid fluorescence activation with significant intensity enhancement within a short time. Furthermore, <strong>LS</strong> featured consistent excitation and emission wavelengths, making it ideal for fluorescence-based detection. (84)</div></div><div><h3>Significance</h3><div>The strong targeting ability, rapid response, and stability of <strong>LS</strong> provide a powerful platform for real-time, dynamic monitoring of PDIA1 activity. This probe holds significant promise for exploring PDIA1's roles in physiological and pathological processes and advancing research in PDIA1-associated diseases using vinyl sulfone-based fluorescent probes.(46)</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115851"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of the ion-exchange functional groups on protein anion-exchange chromatography 离子交换官能团对蛋白质阴离子交换色谱的影响。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-24 DOI: 10.1016/j.ab.2025.115849

Tingyu Li , David Vanderah

引用次数: 0

Technical approaches for breath aldehyde biomarker detection and disease diagnosis: A review 呼吸醛生物标志物检测与疾病诊断技术途径综述

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-19 DOI: 10.1016/j.ab.2025.115841

Taha Kafili-Hajlari , Abdolhossein Naseri , Atefeh Ansarin , Farzaneh Rasoulzadeh

{"title":"Technical approaches for breath aldehyde biomarker detection and disease diagnosis: A review","authors":"Taha Kafili-Hajlari , Abdolhossein Naseri , Atefeh Ansarin , Farzaneh Rasoulzadeh","doi":"10.1016/j.ab.2025.115841","DOIUrl":"10.1016/j.ab.2025.115841","url":null,"abstract":"<div><div>Exhaled breath analysis holds promise as a non-invasive approach for disease diagnosis. Aldehydes represent a class of volatile organic compounds with diagnostic potential as breath biomarkers for cancers and other conditions. However, aldehydes exist at low concentrations in breath and have stability challenges. This review summarizes recent studies on breath aldehyde analysis, focusing on sample collection methodology, analytical techniques implemented, and key findings regarding aldehyde alterations in disease. Breath collection methods examined include commercial bags, end-tidal sampling devices, condensates, and direct analysis. Analytical techniques evaluated gas chromatography, mass spectrometry, and microextraction approaches. Emerging microextraction and sensing technologies are advancing real-time, non-invasive aldehyde detection. Overall, breath aldehyde biomarkers offer immense potential for diagnosis and screening, but continued research is needed to address current limitations. This review provides insights to guide future efforts focused on exhaled aldehyde analysis and disease detection.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115841"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of GDF15 time-resolved fluorescence immunoassay and its clinical application in colorectal cancer GDF15时间分辨荧光免疫分析法的建立及其在结直肠癌中的临床应用。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-19 DOI: 10.1016/j.ab.2025.115848

Meichun Chen , Hongming Fang , Shang Gao , Tianyu Zheng , Shangbin Kao , Yuan Qin , Xueqin Zhao , Xiumei Zhou , Bao Zhu , Biao Huang

{"title":"Establishment of GDF15 time-resolved fluorescence immunoassay and its clinical application in colorectal cancer","authors":"Meichun Chen , Hongming Fang , Shang Gao , Tianyu Zheng , Shangbin Kao , Yuan Qin , Xueqin Zhao , Xiumei Zhou , Bao Zhu , Biao Huang","doi":"10.1016/j.ab.2025.115848","DOIUrl":"10.1016/j.ab.2025.115848","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to develop a highly sensitive time-resolved fluoroimmunoassay for growth differentiation factor 15 (GDF15-TRFIA) and investigate its clinical applicability in colorectal cancer (CRC).</div></div><div><h3>Methods</h3><div>Using the principle of double-antibody sandwich immunity, the GDF15-TRFIA was established by solid-phase capture antibody and labeled detection antibody with europium as a tracer, the levels of serum GDF15 were quantified in healthy controls (HCs) and patients, and the value of GDF15 in the diagnosis of CRC was analyzed.</div></div><div><h3>Results</h3><div>The established method has a wide measurement range and good linearity. The HOOK effect was not observed when GDF15 was less than 2000 ng/mL. The intra-analytical coefficients of variation (CVs) were 3.27 %–4.54 %, and the inter-analytical CVs were 5.84 %–10.41 %, and recoveries were 88.15 %–112.36 %. The correlation between GDF15-TRFIA and ELISA was good (<em>ρ</em> = 0.9284). Serum GDF15 levels were significantly higher in CRC patients than in benign colorectal tumor (BCT) patients and HCs (<em>P</em> < 0.0001). ROC analysis showed that simultaneous detection of CEA, CA19-9, and GDF15 significantly improved the diagnostic efficiency of CEA and CA19-9.</div></div><div><h3>Conclusion</h3><div>A highly sensitive GDF15-TRFIA method for serum GDF15 was successfully established. It can be used for preliminary diagnosis of CRC, and expected to be a good auxiliary tool for the future clinical diagnosis of CRC.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115848"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative determination of human milk oligosaccharides in faecal matter 粪便中人乳寡糖的定量测定。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-15 DOI: 10.1016/j.ab.2025.115845

Thierry Bénet , Adrien Dardinier , Hanne L.P. Tytgat , Sean Austin

{"title":"Quantitative determination of human milk oligosaccharides in faecal matter","authors":"Thierry Bénet , Adrien Dardinier , Hanne L.P. Tytgat , Sean Austin","doi":"10.1016/j.ab.2025.115845","DOIUrl":"10.1016/j.ab.2025.115845","url":null,"abstract":"<div><div>Human milk oligosaccharides (HMOs) are a major component of human milk and colostrum, yet they are non-digestible and thus not utilized directly by the infant. Nevertheless, they are important for infant health and development and have been implicated in immune development, pathogen deflection, cognitive development and the development of healthy microbiome. To understand how HMOs may be utilized it is important to be able to measure them both in milk and faeces. Many methods for the determination of HMOs in milk have been published. However, there are fewer reports of methods describing the quantitative determination of oligosaccharides in faeces. Here we report a validated method for the determination of 30 oligosaccharides in faeces. Oligosaccharides are labelled with 2-aminobenzamide and determined by liquid chromatography with fluorescence detection. The method precision determined as relative standard deviation under intermediate reproducibility conditions is below 12 % for all of the oligosaccharides. Recoveries were in the range 86.6–115 % for the 8 oligosaccharides for which quantitative standards were available, and are estimated to be in the range 81–117 % when using 2′-fucosyllactose as a universal calibrant assuming equimolar response factors of the 2-aminobenzamide labelled oligosaccharides.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115845"},"PeriodicalIF":2.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in aptamers as promising nanotool for therapeutic and diagnostic applications 适体作为治疗和诊断应用的纳米工具的最新进展。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-14 DOI: 10.1016/j.ab.2025.115844

Omar Awad Alsaidan

引用次数: 0

Molecularly imprinted polymers-ZnS quantum dots based composite sensor for optical detection of chlorogenic acid 基于分子印迹聚合物- zns量子点的绿原酸光学检测复合传感器

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-14 DOI: 10.1016/j.ab.2025.115846

Himshweta , Neelam Verma , Nitu Trehan , Minni Singh

{"title":"Molecularly imprinted polymers-ZnS quantum dots based composite sensor for optical detection of chlorogenic acid","authors":"Himshweta , Neelam Verma , Nitu Trehan , Minni Singh","doi":"10.1016/j.ab.2025.115846","DOIUrl":"10.1016/j.ab.2025.115846","url":null,"abstract":"<div><div>Chlorogenic acid (CGA), a key phenolic acid found in coffee, fruits, vegetables, and herbs, has significant pharmacological activities, necessitating its accurate detection in complex matrices. In this study, an organic acrylate molecularly imprinted polymers-chitosan modified zinc sulphide quantum dots/polydopamine (MIPs-CS:ZnS QDs/PDA) based composite sensor for the detection of CGA has been designed. In MIPs shell, CGA served as template and 4-vinylpyridine and methacrylic acid as functional monomers, azobisisobutyronitrile acting as the initiator and ethylene glycol dimethacrylate as the cross-linker. Chitosan was incorporated to enhance the stability of ZnS QDs, while polydopamine was introduced during polymerization to improve adhesion and the selectivity of MIPs for CGA. Under ideal conditions, the composite sensor had shown a linear range of 0.02–11 μg/mL with detection limit of 8.9 × 10<sup>−3</sup> μg/mL. The composite sensor showed imprinting factor of 6.3, and response time of 12 min. The sensor demonstrated good selectivity towards CGA, in the presence of interfering agents. Composite sensor was successfully applied to detect CGA in plant extracts, coffee and fruit juices, with recovery ranges from 88.93 to 98.49 %. The MIPs-CS:ZnS QDs/PDA composite sensor offers a simple and robust approach for CGA detection in real samples without requiring pre-treatment.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115846"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive electrochemiluminescent detection of hydroquinone using silver/luminol-functionalized carbon microspheres 银/鲁米诺功能化碳微球电化学发光对对苯二酚的灵敏检测。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-12 DOI: 10.1016/j.ab.2025.115842

Hui Zhang, Ziqi Wang, Yahui Ji, Junfeng Li, Feifei Chen, Fangxin Du, Gen Liu

引用次数: 0

A simple, rapid, cost-effective and reliable molecular technique for early sex determination in Phoenix dactylifera 一种简单、快速、经济、可靠的凤尾草早期性别测定分子技术。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-12 DOI: 10.1016/j.ab.2025.115843

Asmita Detroja, Jaykumar Koradiya, Munir Ibrahim, Avani Bhimani, Tirth Chetankumar Bhatt, Gaurav Sanghvi, Ashok Kumar Bishoyi

{"title":"A simple, rapid, cost-effective and reliable molecular technique for early sex determination in Phoenix dactylifera","authors":"Asmita Detroja, Jaykumar Koradiya, Munir Ibrahim, Avani Bhimani, Tirth Chetankumar Bhatt, Gaurav Sanghvi, Ashok Kumar Bishoyi","doi":"10.1016/j.ab.2025.115843","DOIUrl":"10.1016/j.ab.2025.115843","url":null,"abstract":"<div><div>The <em>Phoenix dactylifera</em> is an important horticultural cash crop in arid and semi-arid regions, known for its sweet, edible, nutritious fruit. Date palm is a diecious plant; females are commercially important to produce fruits, and males are required for pollination. Dates are easily segregated through seeds, then seed-raised plants revealed 50 % of each male and female plant. But a farmer needs a 95:5 ratio of female to male in the farming land. However, the gender of the plant can be identified four to five years after planting when it begins to flower, and there is no other method available for the identification of the gender. Hence, the present investigation focused on the development of techniques for the early sex determination of dates. Seventy-nine ISSR primers were used to analyze DNA from bulked female and male date palms. Among 79 markers, one marker, ISSR-49, has been selected and successfully validated in 105 female and 103 male date palm samples. The novel reliable biomarker (TG)8RC alone generates both female (400 bp) and male (600 bp) specific, unique amplicons. In conclusion, this investigation developed a simple, efficient, cost-effective technique that can discriminate the gender of date palms in a single PCR reaction within 5 h.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115843"},"PeriodicalIF":2.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric and fluorescent dual-modality assay for cell-free mitochondrial DNA copy number in saliva 唾液中无细胞线粒体DNA拷贝数的比色和荧光双模分析

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2025-03-11 DOI: 10.1016/j.ab.2025.115840

Jiaxu Wang, Zhengrong Lu, Zhanmin Liu, Qiming Chen

{"title":"Colorimetric and fluorescent dual-modality assay for cell-free mitochondrial DNA copy number in saliva","authors":"Jiaxu Wang, Zhengrong Lu, Zhanmin Liu, Qiming Chen","doi":"10.1016/j.ab.2025.115840","DOIUrl":"10.1016/j.ab.2025.115840","url":null,"abstract":"<div><div>Copy number of cell-free mitochondrial DNA (cf-mtDNA) has garnered significant attention as a biomarker for studying and diagnosing various diseases. However, Quantitative Real-time PCR (qPCR) and Droplet Digital PCR (ddPCR) assays for cf-mtDNA copy number detection require expensive equipment and high experiment conditions. In this study, a colorimetric and fluorescent dual-modality assay was developed for quantitative detection of cf-mtDNA copy number. With G-quadruplex (G4) sequence modified primers, the assay could quantitatively detect cf-mtDNA with spectrophotometry, RGB (Red, Green, Blue) visual method and fluorescence method, which made the application scenarios more diverse. The specificity of dual-mode method was better, and the detection limits of spectrophotometry, RGB visual method and fluorescence method were as low as 1.45 × 10<sup>−1</sup> copies/μL, 1.65 copies/μL and 1.58 × 10<sup>−1</sup> copies/μL, respectively. Compared with qPCR and ddPCR assays developed in previous studies, the dual-modality assay in this study had a lower detection limit. It was also independent of expensive qPCR and ddPCR equipment and the detection cost was low. Therefore, the colorimetric and fluorescent dual-modality assay represent a label-free and sensitive approach for assessing cf-mtDNA levels, offering promising implications for biomedical research and clinical diagnostics.</div></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":"702 ","pages":"Article 115840"},"PeriodicalIF":2.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0