Analytical biochemistry最新文献_第4页

Separation of recombinant erythropoietin and human serum albumin without the use of sophisticated equipment 无需复杂设备即可分离重组促红细胞生成素和人血清白蛋白

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-09-13 DOI: 10.1016/j.ab.2024.115673

Dmitry I. Zybin , Anatoly A. Klishin , Natalia V. Orlova , Dmitry А. Zyryanov , Anna I. Prostyakova , Dmitry V. Kapustin

引用次数: 0

Electrocatalytic efficiency of carbon nitride supported gold nanoparticle based sensor for iodide and cysteine detection 基于氮化碳支撑金纳米粒子的碘化物和半胱氨酸检测传感器的电催化效率

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-09-10 DOI: 10.1016/j.ab.2024.115660

Chandan Saha , Sarit K. Ghosh , Pooja Kumari , Venkata K. Perla , Harishchandra Singh , Kaushik Mallick

{"title":"Electrocatalytic efficiency of carbon nitride supported gold nanoparticle based sensor for iodide and cysteine detection","authors":"Chandan Saha , Sarit K. Ghosh , Pooja Kumari , Venkata K. Perla , Harishchandra Singh , Kaushik Mallick","doi":"10.1016/j.ab.2024.115660","DOIUrl":"10.1016/j.ab.2024.115660","url":null,"abstract":"<div><p>Extensive investigations are being conducted on gold nanoparticles focusing on their applications in biosensors, laser phototherapy, targeted drug delivery and bioimaging utilizing advanced detection techniques. In this work, an electrochemical sensor was developed based on graphite carbon nitride supported gold nanoparticles. Carbon nitride supported gold nanoparticles (Au–CN) was synthesized by applying a deposition-precipitation route followed by a chemical reduction technique. The composite system was characterized by X-ray diffraction and X-ray photo electron spectroscopy methods. Electron microscopy analysis confirmed the formation of gold nanoparticles within the size range of 5–15 nm on the carbon nitride support. Carbon nitride supported gold based sensor was employed for the electrochemical detection of iodide ion and <span>l</span>-cysteine. The limit of detection and sensitivity of the sensor was attained 8.9 μM and 0.96 μAμM⁻<sup>1</sup>cm⁻<sup>2</sup>, respectively, for iodide ion, while 0.48 μM and 5.8 μAμM⁻<sup>1</sup>cm⁻<sup>2</sup>, respectively, was achieved for the recognition of cysteine. Furthermore, a paper-based electrochemical device was developed using the Au–CN hybrid system that exhibited promising results in detecting iodide ions, highlighting its potential for economic and portable device applications.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice 开发双抗体夹心酶联免疫吸附测定法，用于定量检测水稻中突变的 EPSPS 基因表达量

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-09-10 DOI: 10.1016/j.ab.2024.115669

Biao Luo , Xianwen Zhang , Fang Wang , Yan Wang , Wei Wu , Chaoyang Lin , Liqun Rao , Qiming Wang

{"title":"Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice","authors":"Biao Luo , Xianwen Zhang , Fang Wang , Yan Wang , Wei Wu , Chaoyang Lin , Liqun Rao , Qiming Wang","doi":"10.1016/j.ab.2024.115669","DOIUrl":"10.1016/j.ab.2024.115669","url":null,"abstract":"<div><p>Glyphosate resistance is a critically important trait for genetically modified (GM) crops. Mutation of the rice EPSPS gene results in a high level of glyphosate resistance, presenting significant potential for the development of glyphosate-tolerant crops. The resistance of rice to glyphosate is correlated with the expression levels of resistance genes. Therefore, developing a convenient, stable, and sensitive method for quantifying the OsmEPSPS protein is crucial for the development of glyphosate-resistant crops. We developed a double-antibody sandwich quantitative ELISA (DAS-ELISA) using a specific monoclonal antibody (mAb) for OsmEPSPS capture and an HRP-conjugated <em>anti</em>-OsmEPSPS rabbit polyclonal antibody (pAb). The method could be used to detect OsmEPSPS within a linear range of 16–256 ng/mL with robust intra- and inter-batch duplicability (%CV values: 0.17 %–7.24 %). OsmEPSPS expression in the transgenic rice lines (54.44–445.80 μg/g) was quantified using the DAS-ELISA. Furthermore, the expression of the <em>OsmEPSPS</em> gene was validated through Western blotting. This study demonstrated the reliability and stability of the DAS-ELISA for OsmEPSPS detection in GM rice.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative approaches in invertase immobilization: Utilizing green synthesized zinc oxide nanoparticles to improve biochemical properties 转化酶固定化的创新方法：利用绿色合成的氧化锌纳米粒子改善生化特性

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-09-07 DOI: 10.1016/j.ab.2024.115661

Somayeh Sadi , Marzieh Ghollasi , Khadijeh Eskandari , Elahe Darvishi

{"title":"Innovative approaches in invertase immobilization: Utilizing green synthesized zinc oxide nanoparticles to improve biochemical properties","authors":"Somayeh Sadi , Marzieh Ghollasi , Khadijeh Eskandari , Elahe Darvishi","doi":"10.1016/j.ab.2024.115661","DOIUrl":"10.1016/j.ab.2024.115661","url":null,"abstract":"<div><p>Invertase enzyme can effectively improve the taste, color, and durability of these products. Various methods have been proposed to increase the stability and efficiency of enzymes. One of the most important is enzyme immobilization, which can be implemented on different materials. The purpose of this study was to immobilize the invertase enzyme on the surface of green synthesized zinc oxide nanoparticles and to investigate its biochemical properties. The enzyme immobilization was confirmed by SEM and Raman spectroscopy. Then, the biochemical characteristics, such as optimal pH and temperature, thermal stability, and storage stability of free and immobilized enzymes, were determined. The results of SEM showed that the diameter of synthesized nanoparticles was about 60 ± 5 nm. FTIR of immobilized invertase confirmed the immobilization process. The immobilization efficiency was determined to be 72 %. Immobilized enzyme showed higher thermal stability at 40 and 50 °C. Immobilized enzyme could be used 8 times in optimum condition. Also, an Examination of the kinetic parameters of the immobilized enzyme compared with those of the free enzyme showed a decrease in the maximum velocity of the enzyme. It seems that the immobilized invertase has improved characteristics for application in different industries.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142163320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basic strategies for monitoring lipase activity: A review 监测脂肪酶活性的基本策略：综述。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-09-06 DOI: 10.1016/j.ab.2024.115659

Ayda Torchi, Hanen Ghamgui, Slim Cherif

引用次数: 0

An RNA aptamer photoelectrochemical biosensor based on the exciton energy transfer constructed for theophylline detection 一种基于激子能量转移的 RNA 合子光电化学生物传感器，用于检测茶碱。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-09-05 DOI: 10.1016/j.ab.2024.115658

Jiexia Chen , Yilin Lu , Gang Zhu , Chuanqi Zhang , Zhuoer Liu , Dexiang Feng , Yan Wei , Lihua Li

{"title":"An RNA aptamer photoelectrochemical biosensor based on the exciton energy transfer constructed for theophylline detection","authors":"Jiexia Chen , Yilin Lu , Gang Zhu , Chuanqi Zhang , Zhuoer Liu , Dexiang Feng , Yan Wei , Lihua Li","doi":"10.1016/j.ab.2024.115658","DOIUrl":"10.1016/j.ab.2024.115658","url":null,"abstract":"<div><p>A novel photoelectrochemical (PEC) biosensor was developed incorporating a specifically designed RNA aptamer for the detection of theophylline (TP). This involved utilizing two nucleotide base aptamers with tailored sequences designed to target TP. The 3′ end of a single-stranded RNA sequence (5′-GGAUACCA–(CH<sub>2</sub>)<sub>6</sub>–SH-3′) and the 5′ end of a complementary stranded RNA sequence (5′–HS–(CH<sub>2</sub>)<sub>6</sub>-CCUUGGAAGCC-3′) were linked to gold nanoparticles (AuNPs) and CdS quantum dots (QDs), respectively. These two single-stranded RNAs (ssRNA) formed a double-stranded RNA (dsRNA) capable of recognizing TP. This major structural change altered the spacing between QDs and NPs, which signaled the presence and concentration of TP. TP was photoelectrochemical catalytic oxidation by the hole of CdS QDs under illumination, then anode photocurrent was generated. Due to the increase in surface impedance and the effect of exciton energy transfer (EET) between QDs and AuNPs, the photocurrent would undergo varying degrees of change. TP was detected by changes in photocurrent. PEC detection of TP was achieved in the range of 0.1 μM–200 μM. The detection limit was 0.033 μM. The method exhibited commendable reproducibility and remarkable selectivity. The biosensor was used to measure TP content in tea, beverages and blood samples, resulting in satisfactory recovery rates.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potentiometric determination of the local anesthetic procaine in pharmaceutical samples 药物样品中局部麻醉剂普鲁卡因的电位测定。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-08-30 DOI: 10.1016/j.ab.2024.115657

Oguz Özbek , Onur Cem Altunoluk

{"title":"Potentiometric determination of the local anesthetic procaine in pharmaceutical samples","authors":"Oguz Özbek , Onur Cem Altunoluk","doi":"10.1016/j.ab.2024.115657","DOIUrl":"10.1016/j.ab.2024.115657","url":null,"abstract":"<div><p>In this study, a new potentiometric sensor was developed for the determination of the local anesthetic drug procaine in pharmaceutical samples. Procaine (Pr)–Tetraphenlyborate (TPB) ion–pair was synthesized and used as a sensor material. Potentiometric sensors using the synthesized ion pair (Pr–TPB), poly(vinyl chloride) (PVC) and <em>o</em>–nitrophenyloctyl ether (<em>o</em>–NPOE) in different proportions were prepared and their performance properties were tested. Among the prepared sensors, the best potentiometric response characteristics were obtained with the sensor composition Pr–TPB:PVC:<em>o</em>–NPOE in the ratio of 6.0:32.0:62.0 (w/w %). The new procaine sensor developed in the present study had a near–Nernstian behavior of 54.1 ± 3.3 mV/per decade and a low detection limit of 3.18 × 10<sup>−5</sup> mol L<sup>−1</sup> in the concentration range of 1.0 × 10<sup>−1</sup>–1.0 × 10<sup>−4</sup> mol L<sup>−1</sup>. Additionally, the sensor had a response time of less than 10 s and could work in a wide pH range for two different concentration values without being affected by pH changes. Finally, the new procaine potentiometric sensor was used to detect procaine in injection samples and successfully determined procaine concentrations with high recoveries.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sandwich enzyme-linked aptamer-based assay for the detection of Trichomonas vaginalis 基于酶联适配体的夹心法检测阴道毛滴虫。

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-08-30 DOI: 10.1016/j.ab.2024.115656

Christine Aubrey C. Justo , Miriam Jauset-Rubio , Marketa Svobodova , Vasso Skouridou , Piet Cools , Guy Mulinganya , Alexandra Ibáñez-Escribano , Windell L. Rivera , Ciara K. O'Sullivan

{"title":"Sandwich enzyme-linked aptamer-based assay for the detection of Trichomonas vaginalis","authors":"Christine Aubrey C. Justo , Miriam Jauset-Rubio , Marketa Svobodova , Vasso Skouridou , Piet Cools , Guy Mulinganya , Alexandra Ibáñez-Escribano , Windell L. Rivera , Ciara K. O'Sullivan","doi":"10.1016/j.ab.2024.115656","DOIUrl":"10.1016/j.ab.2024.115656","url":null,"abstract":"<div><p>Trichomoniasis is the most prevalent curable, non-viral sexually transmitted infection (STI), with an estimated 156 million new infections in 2020. It can potentially result in adverse birth outcomes as well as infertility in men, whilst it also increases the risk of acquiring HIV and contracting other vaginal infections. It is mostly prevalent among women in low-income countries and especially in Africa and the Americas. This STI is caused by <em>Trichomonas vaginalis</em> (TV) and a robust, cost-effective, sensitive, specific and rapid diagnostic test is urgently required. We report the screening of 6 full-length and 4 truncated aptamers previously selected in our group for use in a microplate-based sandwich assay. The combination of dual aptamers comprising a short 14-mer truncated capture aptamer (termed A1_14mer) and a full-length non-truncated reporter aptamer (A6) was elucidated to be the optimum pair for a sensitive sandwich enzyme-linked aptamer assay (ELAA) for the detection of TV achieving a detection limit of 3.02 × 10<sup>4</sup> TV cells/mL. The results obtained with the A1_14mer-A6 ELAA correlate excellently with wet-mount microscopy for the detection of TV in clinical specimens, cervicovaginal lavages and vaginal swabs, highlighting the potential clinical application of this assay for cost-effective population screening and subsequent prevention of the onset of complications associated with undiagnosed and untreated TV.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003269724002008/pdfft?md5=822f4d856d2fbf00baa6ae64cb17eb48&pid=1-s2.0-S0003269724002008-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of ssDNA aptamers against BipD antigen of Burkholderia pseudomallei 针对假丝酵母伯克霍尔德氏菌 BipD 抗原的 ssDNA 短肽的分离与表征

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-08-28 DOI: 10.1016/j.ab.2024.115655

Kasturi Selvam , Mohamad Ahmad Najib , Muhammad Fazli Khalid , Muhammad Hafiznur Yunus , Habibah A. Wahab , Azian Harun , Ummu Afeera Zainulabid , Khairul Mohd Fadzli Mustaffa , Ismail Aziah

{"title":"Isolation and characterization of ssDNA aptamers against BipD antigen of Burkholderia pseudomallei","authors":"Kasturi Selvam , Mohamad Ahmad Najib , Muhammad Fazli Khalid , Muhammad Hafiznur Yunus , Habibah A. Wahab , Azian Harun , Ummu Afeera Zainulabid , Khairul Mohd Fadzli Mustaffa , Ismail Aziah","doi":"10.1016/j.ab.2024.115655","DOIUrl":"10.1016/j.ab.2024.115655","url":null,"abstract":"<div><h3>Background</h3><p>Melioidosis is difficult to diagnose due to its wide range of clinical symptoms. The culture method is time-consuming and less sensitive, emphasizing the importance of rapid and accurate diagnostic tests for melioidosis. <em>Burkholderia</em> invasion protein D (BipD) of <em>Burkholderia pseudomallei</em> is a potential diagnostic biomarker. This study aimed to isolate and characterize single-stranded DNA aptamers that specifically target BipD.</p></div><div><h3>Methods</h3><p>The recombinant BipD protein was produced, followed by isolation of BipD-specific aptamers using Systematic Evolution of Ligands by EXponential enrichment. The binding affinity and specificity of the selected aptamers were evaluated using Enzyme-Linked Oligonucleotide Assay.</p></div><div><h3>Results</h3><p>The fifth SELEX cycle showed a notable enrichment of recombinant BipD protein-specific aptamers. Sequencing analysis identified two clusters with a total of seventeen distinct aptamers. AptBipD1, AptBipD13, and AptBipD50 were chosen based on their frequency. Among them, AptBipD1 exhibited the highest binding affinity with a <em>K</em><sub>d</sub> value of 1.0 μM for the recombinant BipD protein. Furthermore, AptBipD1 showed significant specificity for <em>B. pseudomallei</em> compared to other tested bacteria.</p></div><div><h3>Conclusion</h3><p>AptBipD1 is a promising candidate for further development of reliable, affordable, and efficient point-of-care diagnostic tests for melioidosis.</p></div>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to “Prediction of lysine HMGylation sites using multiple feature extraction and fuzzy support vector machine” [Anal. Biochem. 663 (2023), 115032] 对 "利用多重特征提取和模糊支持向量机预测赖氨酸 HMGylation 位点 "的勘误 [Anal. Biochem.

IF 2.6 4区生物学

Analytical biochemistry Pub Date : 2024-08-26 DOI: 10.1016/j.ab.2024.115652

Zhe Ju, Shi-Yun Wang

引用次数: 0