Identifying transglutaminase substrate glutaminyls using dansylcadaverine

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-05-07 DOI:10.1016/j.ab.2025.115888

Thomas M. Jeitner , Pradeep K. Singh , Juan Azcona , Chad W. Euler , James M. Kelly

引用次数: 0

Abstract

Dansylcadaverine is often used at a final concentration of 1 mM to identify transglutaminase substrate glutaminyl residues. At this concentration, dansylcadaverine only labels a fraction of the possible substrates. Therefore, we developed a 500 mM stock dansylcadaverine solution in dimethyl sulfoxide:acetic acid (19:1) that allows the identification of all transglutaminase substrate glutaminyl residues. This solution was used to identify these substrate residues: Gln 283, Gln 325, and Gln 677 in a fragment of arginine-specific gingipain A.

查看原文本刊更多论文

用丹西尸碱鉴定转谷氨酰胺酶底物谷氨酰胺

丹西尸碱通常在终浓度为1mm时用于鉴定转谷氨酰胺酶底物谷氨酰基残基。在这个浓度下，丹西尸碱只能标记一小部分可能的底物。因此，我们在二甲基亚砜：乙酸（19:1）中开发了500 mM的丹西尔尸胺原液，可以鉴定所有转谷氨酰胺酶底物谷氨酰基残基。该溶液用于鉴定精氨酸特异性gingipain a片段中的Gln 283、Gln 325和Gln 677底物残基。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.