Streptomycin-modified magnetic beads enable robust DNA extraction across diverse lysis conditions

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-03-05 DOI:10.1016/j.ab.2025.115836

Yanwen Qi , Jiayu Zheng , Yingqiu Xie , Amr Amin , Bing Qiao , Chao Shi , Yong Li , Cuiping Ma

引用次数: 0

Abstract

Here, we describe the applicability of a variety of functionalized magnetic beads (MBs) for DNA extraction under different lysis conditions. Various MBs, including hydroxyl, carboxylic, amino, chitosan-modified, and streptomycin-modified MBs (MBs-STR), were compared based on their extracted DNA quantity and quality. The results presented that the MBs exhibited different DNA adsorption capacities under specific conditions. Notably, MBs-STR maintained stable DNA adsorption capacity upon different conditions through a non-intercalative binding mechanism between STR and DNA minor groove. This discovery advances antibiotic-based DNA extraction strategies, offering a flexible, efficient alternative for biomedical applications while enriching the toolkit for nucleic acid isolation.

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链霉素修饰磁珠能够在不同的裂解条件下进行稳健的DNA提取

在这里，我们描述了各种功能化磁珠（mb）在不同裂解条件下提取DNA的适用性。对羟基、羧基、氨基、壳聚糖修饰和链霉素修饰的MBs （MBs- str）进行了提取DNA数量和质量的比较。结果表明，在特定条件下，MBs表现出不同的DNA吸附能力。值得注意的是，MBs-STR通过与DNA小槽的非插层结合机制，在不同条件下均保持稳定的DNA吸附能力。这一发现推进了基于抗生素的DNA提取策略，为生物医学应用提供了一种灵活、高效的替代方案，同时丰富了核酸分离的工具包。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.