Acta biochimica Polonica最新文献

{"title":"3-O-ethyl-L-ascorbic acid modulates pro-inflammatory cytokine and extracellular matrix-related biomarker levels in human lung fibroblasts: an <i>in vitro</i> study.","authors":"Ali Türkan, Murat Türkoğlu, Gülistan Öncü, Hakan Sevinç","doi":"10.3389/abp.2026.16774","DOIUrl":"10.3389/abp.2026.16774","url":null,"abstract":"<p><p>L-Ascorbic acid (also known as vitamin C) is widely used in dietary supplements and cosmetic formulations because of its antioxidant properties and critical role in numerous physiological processes. In this study, we investigated the effects of 3-O-ethyl-L-ascorbic acid (EAA), a stable vitamin C derivative, on inflammatory and extracellular matrix (ECM)-related biomarkers in MRC-5 human lung fibroblasts. We quantified protein levels of pro-inflammatory cytokines, ECM-associated proteins, and matrix-degrading enzymes using enzyme-linked immunosorbent assay (ELISA)-based assays. EAA treatment significantly reduced the basal levels of interleukin-1 Beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), indicating modulation of inflammatory signaling under unstimulated conditions. EAA also increased the levels of collagen type I (COL-I), collagen type III (COL-III), and hyaluronan synthase 2 (HAS2), suggesting enhanced production of ECM-associated components. In addition, EAA altered the levels of matrix-degrading enzymes in a time-dependent manner. Matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) exhibited a biphasic response characterized by higher levels at 24 h and lower levels at 48 h, whereas elastase levels were consistently reduced. Taken together, these findings suggest that EAA modulates inflammatory, ECM-associated, and ECM-degrading biomarker levels in MRC-5 fibroblasts under the tested <i>in vitro</i> conditions. Further studies are needed to determine whether these changes have functional implications for ECM homeostasis.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16774"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclotorsion as a structural and functional biomarker of ocular and neuroophthalmic diseases.","authors":"Agata Joanna Ordon, Piotr Jerzy Loba","doi":"10.3389/abp.2026.17270","DOIUrl":"https://doi.org/10.3389/abp.2026.17270","url":null,"abstract":"<p><p>Cyclotorsion, defined as rotation of the eye around its anteroposterior axis, is an important but often underrecognized component of ocular motility. This review summarizes the physiological basis of cyclotorsion, methods of subjective and objective assessment, and the clinical relevance of torsional deviations in ocular and neuro-ophthalmic disorders. Particular emphasis is placed on objective measurement using the disc-foveal angle and its role in diagnosing and monitoring conditions such as trochlear nerve palsy, skew deviation, pulley disorders, inferior oblique overaction, thyroid eye disease, heavy eye syndrome, and sagging eye syndrome. Quantitative assessment of cyclotorsion may improve diagnostic accuracy, support surgical planning, and provide an objective marker of treatment outcome.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"17270"},"PeriodicalIF":2.2,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of endothelial-specific molecule-1 levels in patients with bone tumors.","authors":"Eyup Senocak, Nurinnisa Ozturk, Adem Keskin, Umit Aygun, Ibrahim Dag, Muhammet Celik","doi":"10.3389/abp.2026.17417","DOIUrl":"10.3389/abp.2026.17417","url":null,"abstract":"<p><strong>Background: </strong>In suspected bone tumors where imaging and histopathological evaluation are fundamental for diagnosis, minimally invasive blood biomarkers are needed for earlier diagnosis, initial assessment, and risk stratification. The aim of this research was to evaluate the diagnostic value of Endothelial-Specific Molecule-1 (ESM-1) levels in determining the presence of bone tumors and distinguishing between benign and malignant tumors.</p><p><strong>Methods: </strong>This cross-sectional research, conducted at a research hospital, involved 60 participants diagnosed with bone tumors and 30 healthy participants. ESM-1 values were analyzed using Enzyme-Linked Immunosorbent Assay (ELISA). The data obtained were evaluated using binary logistic regression analysis and Receiver Operating Characteristic (ROC) curve analysis.</p><p><strong>Results: </strong>ESM-1 values were determined to be higher in the bone tumors group than in the control group. ROC analysis showed an Area Under the Curve (AUC) of 0.895. Using a cut-off value of 138.94 ng/L for detecting bone tumors, the sensitivity and specificity were 78.3% and 80.0%, respectively. In addition, ESM-1, erythrocyte sedimentation rate, and C-reactive protein levels were found to be higher in the malignant subgroup than in the benign subgroup. In subgroup ROC analysis, ESM-1 perfectly discriminated against malignant tumors from both benign tumors (AUC = 1.000) and healthy controls (AUC = 1.000), with sensitivities and specificities of 100% at cut-off values of 204.54 ng/L and 195.00 ng/L, respectively. For differentiating benign tumors from healthy controls, the AUC was 0.789, with 70.0% sensitivity and 70.0% specificity at a cut-off value of 125.54 ng/L.</p><p><strong>Conclusion: </strong>ESM-1 levels may have potential diagnostic value in distinguishing between bone tumors and malignant tumors; however, these results need to be confirmed in larger samples.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"17417"},"PeriodicalIF":2.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity screening of plant ethanol extracts against <i>Plutella xylostella</i> and field evaluation of an optimized botanical combination formulation.","authors":"Jie Zhang, Xiaoe Wang, Xiaoli Xu, Williamson Gustave, Xiaokai Zhang","doi":"10.3389/abp.2026.16918","DOIUrl":"10.3389/abp.2026.16918","url":null,"abstract":"<p><p><i>Plutella xylostella</i> is a major pest of cruciferous vegetables. The prolonged overuse of chemical insecticides has resulted in increased resistance in <i>P. xylostella</i> populations, highlighting the urgent need for environmentally friendly and effective alternative control strategies. In this study, ethanol extracts from 15 plant species collected from the Qinling Mountains of China were systematically evaluated for their insecticidal, antifeedant, and oviposition-repellent activities against <i>P. xylostella.</i> Laboratory bioassays were conducted using leaf-dipping and leaf-disc methods. Among the tested plants, ethanol extracts of <i>Atropa belladonna</i> and <i>Blumea riparia var. megacephala</i> exhibited the strongest biological activities. Specifically, <i>A. belladonna</i> extract achieved corrected mortality, antifeedant, and oviposition-repellent rates of 83.11%, 89.46%, and 78.57%, respectively, whereas <i>B. riparia var. megacephala</i> extract produced corrected mortality, antifeedant, and oviposition-repellent rates of 73.53%, 87.45%, and 82.14%, respectively. When combined at a volume ratio of 8:2, the two extracts showed enhanced insecticidal activity, with a median lethal concentration (LC50) of 65.762 mg/mL and a co-toxicity coefficient consistent with an additive effect without antagonism. A stable emulsifiable concentrate (EC) formulation was subsequently developed through optimization of the solvent and surfactant system. Field trials demonstrated that the 10% botanical EC effectively suppressed <i>P. xylostella</i> populations, achieving a control efficacy of 58.35% at 7 days post-application, which was comparable to that of the commonly used insecticide emamectin benzoate. Collectively, these findings suggest that <i>A. belladonna</i> and <i>B. riparia var. megacephala</i> have potential as botanical insecticide resources for the integrated management of <i>P. xylostella</i> and may contribute to more sustainable agricultural practices.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16918"},"PeriodicalIF":2.2,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13558121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148928596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological and biochemical alterations in patients with suspected COVID-19 infection with negative and positive RT-PCR swabs.","authors":"Lotfi S Bin Dahman, Adnan A Bakarman, Naela A Al-Nakhbi, Ahmed M Al-Haddad, Ammar H Bin-Talib, Hamzah A Al-Saadi, Suad T Bashatah, Noora S Al-Yazidi, Mohammed S Al-Houti, Mohammed O Bin-Dheyab","doi":"10.3389/abp.2026.16764","DOIUrl":"10.3389/abp.2026.16764","url":null,"abstract":"<p><strong>Background: </strong>Hematological and biochemical abnormalities have been reported in patients with confirmed cases of coronavirus disease 2019 (COVID-19). This study compared selected hematological and biochemical parameters in patients with confirmed COVID-19 (RT-PCR positive) and suspected COVID-19 (RT-PCR negative).</p><p><strong>Patients and methods: </strong>A retrospective cross-sectional study was conducted among 106 patients admitted to the COVID-19 isolation centers at Ibn-Sina Hospital, Mukalla, Yemen, from 1 December 2021 to 30 October 2022. The study included 63 men and 43 women, with a mean age of 58.3 ± 17.5 years. Demographic, clinical, hematological, and biochemical data were extracted from medical records. The following were analyzed: complete blood count (CBC), random blood glucose (RBG), alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), C-reactive protein (CRP), and ferritin.</p><p><strong>Results: </strong>Of the 106 patients, 58 (54.72%) had confirmed (RT-PCR positive) COVID-19, and 48 (45.3%) had suspected (RT-PCR negative) COVID-19. Compared with the RT-PCR-negative group, the RT-PCR-positive patients had significantly higher hemoglobin, hematocrit, red blood cell (RBC), and white blood cell (WBC) counts and were more likely to exhibit neutrophilia, lymphopenia, and eosinopenia. Serum LDH, GGT, CRP, and ferritin levels were also significantly higher in the RT-PCR-positive group. Using binary logistic regression analysis, LDH (OR: 1.008, 95% CI: 1.002-1.013, P = 0.004) and GGT (OR: 0.949, 95% CI: 0.903-0.997, P = 0.039) were independently associated with RT-PCR positivity.</p><p><strong>Conclusion: </strong>Among the investigated laboratory markers, LDH and GGT showed independent associations with RT-PCR positivity. Given the retrospective cross-sectional design, these findings should be interpreted as associations rather than evidence of predictive or causal relationships.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16764"},"PeriodicalIF":2.2,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13529605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary tract infections in diabetes: clinical significance and management approaches.","authors":"Beata Zalewska-Piątek, Michalina Nagórka, Rafał Piątek","doi":"10.3389/abp.2026.17198","DOIUrl":"10.3389/abp.2026.17198","url":null,"abstract":"<p><p>Diabetes mellitus is a significant risk factor for urinary tract infections (UTIs), which occur more frequently, have a more severe course, and show a greater tendency to recur in affected individuals. Metabolic disturbances, particularly hyperglycemia and glucosuria, promote bacterial growth while simultaneously impairing innate and adaptive immune responses. In addition, diabetes-related complications, such as autonomic neuropathy, contribute to bladder dysfunction and urinary stasis, further facilitating bacterial colonization. Epidemiological evidence indicates a two- to four-fold increased risk of UTIs in patients with diabetes, along with a high prevalence of asymptomatic bacteriuria and recurrent infections. A key feature of this relationship is its bidirectional nature. UTIs can exacerbate glycemic dysregulation by triggering stress responses and increasing insulin resistance, leading to transient or sustained hyperglycemia. Uropathogenic <i>Escherichia coli</i> plays a critical role in the pathogenesis of UTIs, with hyperglycemic conditions further enhancing bacterial adhesion, biofilm formation, and immune evasion. Effective management requires simultaneous control of infection and optimization of glycemic control, particularly in the context of rising antimicrobial resistance. Emerging therapeutic strategies include targeting bacterial adhesion, disrupting biofilms, and modulating host immune responses. Improved understanding of host-pathogen interactions may enable the development of more effective preventive and therapeutic approaches in this high-risk population.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"17198"},"PeriodicalIF":2.2,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13526685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gap junction protein connexin 36 proteostatic mechanisms in photoreceptor cells subjected to excitotoxic stress.","authors":"Monika Katan, Klaudia Mróz, Anna Pacwa, Joanna Lewin-Kowalik, Adrian Smędowski","doi":"10.3389/abp.2026.16091","DOIUrl":"10.3389/abp.2026.16091","url":null,"abstract":"<p><p>Connexin 36 (Cx36), a neuronal gap junction protein, is essential for retinal signal transmission but may also contribute to neurodegeneration by facilitating the spread of excitotoxic stress signals. This study aimed to examine the degradation pathway of Cx36 under stress conditions relevant to retinal disease. Using immortalized 661W murine photoreceptor cells transfected with GFP-tagged Cx36, we evaluated the effects of lysosomal inhibition (Bafilomycin A1) and excitotoxic stimulation (NMDA). Immunofluorescence revealed intracellular Cx36 accumulation following Bafilomycin and NMDA exposure, accompanied by increased expression of p62/SQSTM1 and PSMA1, suggesting impaired autophagic flux and a potential compensatory involvement of the proteasome. Elevated RAB11A levels suggested recruitment of endosomal recycling pathways. NMDA treatment was associated with increased ubiquitin accumulation, particularly in Cx36-overexpressing cells, indicating enhanced proteostatic stress under excitotoxic conditions. These findings highlight the stress-responsive behavior of Cx36 and suggest that connexin proteostasis may represent a potential target for future investigation in retinal neurodegeneration, including glaucoma and age-related macular degeneration. Immunofluorescence revealed intracellular accumulation of p62 and PSMA1 after Bafilomycin exposure with signal intensities increasing approximately 1.5-2-fold versus controls (n = 3, p < 0.05). These data are consistent with disrupted autophagic flux and suggest proteasome involvement, but do not constitute functional proof.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16091"},"PeriodicalIF":2.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13523667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of a TEEDA-based initiation system for SDS-PAGE.","authors":"Chaoyang Chen, Mengting Sun, Xiaoxia Ji, Lin Ge, Jia Xu, Mengyuan Cai","doi":"10.3389/abp.2026.17215","DOIUrl":"10.3389/abp.2026.17215","url":null,"abstract":"<p><p>Polyacrylamide gel preparation for SDS-PAGE is conventionally initiated by the ammonium persulfate (APS)-TEMED system. Although effective, the relatively high volatility of TEMED may increase operator exposure during routine laboratory use. In this study, N,N,N',N-tetraethylethylenediamine (TEEDA) was systematically evaluated as an alternative accelerator for SDS-PAGE gel preparation. TEEDA alone produced polymerization and protein separation performance comparable to those of the conventional TEMED-APS system, although higher concentrations (8.3 mM in 10% separating gels and 17.4 mM in 5% stacking gels) were required than those of TEMED (4.0 mM and 6.7 mM, respectively). When TEEDA concentrations comparable to those of TEMED were used, supplementation with 0.1% SBS in separating gels and 0.01% SBS in stacking gels restored polymerization and protein separation performance to levels comparable with those of the conventional TEMED-APS system. These findings provide an optimized TEEDA-based formulation for routine SDS-PAGE.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"17215"},"PeriodicalIF":2.2,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13509136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The yields of nucleic acids and proteins extracted from various murine tissue types.","authors":"Yifan Chen, Xixuan Dong, Lixiang Xue, Zhongnan Yin","doi":"10.3389/abp.2026.16122","DOIUrl":"10.3389/abp.2026.16122","url":null,"abstract":"<p><strong>Objective: </strong>To characterize deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein yields extracted from tissue of fourteen mouse organ types to establish baseline yield estimates for these biomolecules and provide guidance for tissue sample usage and storage in biomedical studies.</p><p><strong>Methods: </strong>Six wild-type C57BL/6J and six BALB/c nude mice were euthanized and fresh tissues were collected for extracting total DNA, RNA, and protein. The concentration, purity, and quality of nucleic acids were measured with NanoDrop™ One spectrophotometer, gel electrophoresis or capillary electrophoresis. Protein concentration was determined using the bicinchoninic acid (BCA) method.</p><p><strong>Results: </strong>DNA yields were highest in the colorectum and lowest in bone. RNA yields were highest in the colorectum and spleen of C57BL/6J and BALB/c nude mice, respectively, and lowest in bone. Protein yields were highest in the kidney and liver of C57BL/6J and BALB/c nude mice, respectively, and lowest in bone. The tissue quantities required to obtain 10 μg of DNA, 10 μg of RNA, and 1 mg of protein were calculated and presented along with corresponding tissue sizes.</p><p><strong>Conclusion: </strong>DNA, RNA, and protein yields varied significantly across different mouse tissue types. These findings provide a reference for estimating tissue sample sizes needed for downstream assays and optimizing the preservation of mouse tissues.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16122"},"PeriodicalIF":2.2,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13461566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148721504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Value evaluation of serum (sdLDLc*HCYc)/HDLc ratio in the stability of intracranial arterial plaques in patients with acute cerebral infarction.","authors":"Hongyu Hao, Xing Xing, Yajing Li, Hongshan Chu, Lei Zhao, Siqi Cheng, Yang Liu, Tiankui Wang, Nan Meng, Ruisheng Duan","doi":"10.3389/abp.2026.17388","DOIUrl":"10.3389/abp.2026.17388","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.18388/abp.2020_6817.].</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"17388"},"PeriodicalIF":2.2,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13461560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}