Acta biochimica Polonica最新文献_第10页

Co-existence of Extended Spectrum β-Lactamase and carbapenemase-producing genes from Diarrheagenic Enteric pathogens isolated in a tertiary care hospital. 三级医院致泻性肠道病原菌中β-内酰胺酶和碳青霉烯酶产谱基因的共存

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-01-25 DOI: 10.18388/abp.2020_6188

Archana A Kharat, Nilesh Makwana, Dlip G Kadam, Anusaya S Chavan, Jeetendra A Kulkarni, Arun S Kharat

{"title":"Co-existence of Extended Spectrum β-Lactamase and carbapenemase-producing genes from Diarrheagenic Enteric pathogens isolated in a tertiary care hospital.","authors":"Archana A Kharat, Nilesh Makwana, Dlip G Kadam, Anusaya S Chavan, Jeetendra A Kulkarni, Arun S Kharat","doi":"10.18388/abp.2020_6188","DOIUrl":"https://doi.org/10.18388/abp.2020_6188","url":null,"abstract":"Multiple drug resistance (MDR) among bacterial pathogens is a growing concern that clinicians are facing worldwide. Diarrhea among infants is frequent and is caused by various bacterial and viral infectious agents. Two hundred and twelve stool specimens were collected from pediatric patients from a rural quaternary hospital in Barshi, Sholapur, India, between March and December 2017. Total 180 specimens were positive for various bacterial pathogens, while the remaining 32 diarrhea cases may have been caused by a viral or uncultured bacterial pathogen. Identification of the bacterium and its antibiotic susceptibility were primarily carried out with VITEK-2. Distribution of diarrhea-causing bacteria among the 180 samples was as follows: 61.11% (110) Escherichia coli, 30.55% (55) Klebsiella pneumoniae, 4.44% (8) Proteus mirabilis, 2.22% (4) Shigella spp. 1.11% (2) Morganella morganii and 0.55% (1) each for Enterobacter cloacae and Citrobacter koseri. There was a co-existence of multiple genetic traits conferring extreme drug resistance (XDR) status to 19 isolates, 17 of which were determined to be E. coli and one each of E. cloacae and C. koseri. Antibiotype determination using VITEK-2 and polymerase chain reaction (PCR) amplification of the genetic traits indicated the co-existence of blaTEM and blaCTX-M15 isolates in all 19 isolates, with the exception of E. cloacae. Results showed that 10 out of 19 strains expressed the AmpC cephalosporinase blaCMY-2 gene, whereas metallo-carbapenemase was expressed in four isolates. Distribution of blaNDM-11 and acquired penicillinase blaSHV-1 resistance among 180 clinical isolates is discussed in the light of ESBL traits. This is the first report from the rural part of Maharashtra India showing that as many as 10.55% of the pathogenic strains were XDR, a step ahead of MDR.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 1","pages":"69-76"},"PeriodicalIF":1.7,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9196859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promoting action of long non-coding RNA small nucleolar RNA host gene 4 in ovarian cancer. 长链非编码RNA小核仁RNA宿主基因4在卵巢癌中的促进作用。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-01-19 DOI: 10.18388/abp.2020_6141

Chao Liu, Shu Zhao, Zhi Xiang Lv, Xiao Juan Zhao

{"title":"Promoting action of long non-coding RNA small nucleolar RNA host gene 4 in ovarian cancer.","authors":"Chao Liu, Shu Zhao, Zhi Xiang Lv, Xiao Juan Zhao","doi":"10.18388/abp.2020_6141","DOIUrl":"https://doi.org/10.18388/abp.2020_6141","url":null,"abstract":"Objective: Long non-coding RNA (LncRNA) small nucleolar RNA host gene 4 (SNHG4) has been shown to be aberrantly expressed in a variety of cancers and involved in cancer development, but its role in ovarian cancer (OC) is unclear. The purpose of this study was to explore the biological function of SNHG4 in OC and reveal its potential downstream molecular targets.Methods: OC tumor tissue and normal tissue were collected; normal human ovarian epithelial cell line (IOSE80) and human ovarian cancer cell line (A2780, SKOV-3, OV-90 and CAOV3) were selected. RT-qPCR was used to detect SNHG4, miR-98-5p, and TMED5, while western blot was used to detect the protein expression levels of TMED5, Ki67, MMP-9, Bcl-2, Bax, Gsk3β, Wnt3a, and β-catenin. The subcellular localization of SNHG4 was assessed by nucleocytoplasmic separation assay. CCK-8, colony formation assay, flow cytometry, and Transwell were used to assess the biological behavior of OC cells. The targeting relationship between SNHG4, miR-98-5p and TMED5 was verified by dual luciferase reporter assay and RIP assay.Results: In OC, SNHG4 and TMED5 were highly expressed, and miR-98-5p was underexpressed. Knockdown of SNHG4 inhibited OC cell proliferation, migration and invasion, promoted apoptosis, and prevented Wnt/β-catenin pathway activation. The effect of knockdown of SNHG4 was reversed by knockdown of miR-98-5p or overexpression of TMED5. Mechanistically, SNHG4 competitively adsorbed miR-98-5p to mediate TMED5 expression, thereby activating the Wnt/β-catenin pathway.Conclusion: SNHG4 accelerates OC development via mediating the miR-98-5p/TMED5 axis and activating the Wnt/β-Catenin pathway. SNHG4 gene silencing might be a novel option for OC treatment.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 1","pages":"59-68"},"PeriodicalIF":1.7,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9136471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Placental expression of Fatty Acid Desaturases 1, 2 and 3 in selected pregnancy pathologies. 脂肪酸去饱和酶1、2和3在妊娠病理中的胎盘表达。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-01-18 DOI: 10.18388/abp.2020_6345

Rafał Bobiński, Urszula Mazurek, Nikola Zmarzly, Izabela Ulman-Włodarz, Mieczysław Dutka, Monika Pizon, Wioletta Pollok-Waksmańska, Anna Pielesz, Maciej Hajduga, Karolina Zimmer, Jan Bujok, Celina Pająk, Tomasz Ilczak

{"title":"Placental expression of Fatty Acid Desaturases 1, 2 and 3 in selected pregnancy pathologies.","authors":"Rafał Bobiński, Urszula Mazurek, Nikola Zmarzly, Izabela Ulman-Włodarz, Mieczysław Dutka, Monika Pizon, Wioletta Pollok-Waksmańska, Anna Pielesz, Maciej Hajduga, Karolina Zimmer, Jan Bujok, Celina Pająk, Tomasz Ilczak","doi":"10.18388/abp.2020_6345","DOIUrl":"https://doi.org/10.18388/abp.2020_6345","url":null,"abstract":"Intrauterine development is a key period in human life. The foetal progress largely depends on the function of the placenta, whose responsibility is transportation and biosynthesis of fatty acids. Desaturation enzymes play a key role in placental fatty acid metabolism. Expression of genes coding for desaturases may be associated with pregnancy abnormalities. The objective of this study was to determine the transcriptional activity of the placental genes Fatty Acid Desaturases 1, 2 and 3 (FADS 1, 2 and 3) in women who gave birth to the infants appropriate for gestational age, large for gestational age, small for gestational age, with intrauterine growth restriction and born preterm. 34 pregnant women aged 21-37 years old participated in the study. The placental samples were taken from a site located 2-3 cm away from the umbilical cord attachment. The collected tissue sections were stored in RNAlater according to the manufacturer's protocol, until required for molecular analysis. The expression profiles of FADS1, FADS2 and FADS3 were determined with RT-qPCR. There was no difference in FADS1 and FADS2 expression between the groups. However, the differences in the expression of the FADS3 were found. Analysis of the FADS1, FADS2 and FADS3 transcription showed significant differences between most of the examined groups. Our findings suggest that the transcriptional activity of FADS genes changes with the severity of intrauterine disorders and is associated with foetal lipid disorders linked to a greater accumulation of fat in the foetal tissues.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 1","pages":"137-144"},"PeriodicalIF":1.7,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9143288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular cloning of glutathione reductase from Oryza sativa, demonstrating its peroxisomal localization and upregulation by abiotic stresses. 水稻谷胱甘肽还原酶的分子克隆及其在非生物胁迫下的过氧化物酶体定位和上调。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-01-08 DOI: 10.18388/abp.2020_6441

Pamela Chanda Roy, Gopal Chowdhary

引用次数: 0

Lipid disorders - the comparison between general population and haemodialyzed patients. Will the Oral Fat Tolerance Test improve the diagnostic? 血脂紊乱——普通人群与血液透析患者的比较。口服脂肪耐受试验能提高诊断吗?

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2022-12-15 DOI: 10.18388/abp.2020_6462

Aleksandra Cader, Joanna Stępniewska, Jacek Różański

{"title":"Lipid disorders - the comparison between general population and haemodialyzed patients. Will the Oral Fat Tolerance Test improve the diagnostic?","authors":"Aleksandra Cader, Joanna Stępniewska, Jacek Różański","doi":"10.18388/abp.2020_6462","DOIUrl":"https://doi.org/10.18388/abp.2020_6462","url":null,"abstract":"The cardiovascular events are frequent complications in chronic kidney disease (CKD). In the general population the risk factors of CV disease are well established and divided into two groups: non-modifiable, and modifiable. The best-known modifiable risk factors leading to the atherosclerotic plaque formation are lipid disorders. In comparison, an association between serum lipid profile in haemodialyzed patients and cardiovascular mortality is more complex and still unclear. Furthermore, it is important to note that recent studies suggest an inverse relationship between lipid disorders and CV mortality in a haemodialyzed population called 'reverse epidemiology'. The disparity between the general and haemodialyzed populations may be supported by the fact that the haemodialysis process itself contributes to the development of dyslipidaemia. Moreover, the chronic kidney disease is associated with metabolic abnormalities which can increase the risk of CVD occurrence. It is estimated that one-third of the patients on haemodialysis have lipid profile abnormalities, the most common one is hypertriglyceridemia. The assessment of the lipid profile has so far been performed in a fasting and non-fasting (postprandial) state, but both of these methods have some limitations. This review evaluates the current knowledge about lipid profile abnormalities in haemodialyzed patients and discusses a potential role of the Oral Fat Tolerance Test (OFTT) as a new tool in clinical practice that may improve the diagnosis of postprandial hypertriglyceridemia.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"69 4","pages":"691-696"},"PeriodicalIF":1.7,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10751894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DUSP28 promotes cell proliferation, migration, and invasion by Akt/β-catenin/Slug axis in breast cancer. DUSP28通过Akt/β-catenin/Slug轴促进乳腺癌细胞增殖、迁移和侵袭。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2022-12-15 DOI: 10.18388/abp.2020_5843

Jiabo Zhang, Yu Guo

{"title":"DUSP28 promotes cell proliferation, migration, and invasion by Akt/β-catenin/Slug axis in breast cancer.","authors":"Jiabo Zhang, Yu Guo","doi":"10.18388/abp.2020_5843","DOIUrl":"https://doi.org/10.18388/abp.2020_5843","url":null,"abstract":"Background: Breast cancer (BCa) has been the most commonly diagnosed cancer worldwide and the leading cause of cancer-related death. Dual-specificity phosphatase 28 (DUSP28) is associated with various cancer progression, but its function and mechanism in breast cancer remain unclear.Methods: DUSP28 level was identified by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assays. The proliferation, migration, and invasion of DUSP28 in MCF-7 and MDA-MB-231 cells were assessed by Cell Counting kit-8 (CCK-8), colony formation, and transwell assays. The xenograft tumor model was established to explore the effects of DUSP28 on tumor growth of nude mice. Immunohistochemistry (IHC) and western blot assays were performed to evaluate the expression of related signal molecules.Results: The expression of DUSP28 was up-regulated in BCa tissues and closely correlated with tumor size and distant lymphatic metastasis in The Cancer Genome Atlas (TCGA) dataset. Quantitative real-time PCR and western blot assays indicated that the expression of DUSP28 was up-regulated in BCa cells. DUSP28 was demonstrated to promote the proliferation, migration, and invasion of MCF-7 and MDA-MB-231 cells in vitro. Knockdown of DUSP28 inhibited tumor growth of xenograft tumor mice in vivo and reduced the levels of DUSP28 and Ki-67. Notably, further mechanism analysis indicated that DUSP28 promoted the activation of Akt/β-catenin/Slug signaling.Conclusion: DUSP28 exerts its oncogene function via regulating Akt/β-catenin/Slug signaling in BCa, indicating that DUSP28 may provide a promising therapeutic target for the treatment of BCa.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"69 4","pages":"711-717"},"PeriodicalIF":1.7,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10458590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Up-regulation of HSPA1A and HSPA1B in the blood of tophi patients and its clinical significance. 痛风患者血中HSPA1A和HSPA1B的上调及其临床意义。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2022-12-14 DOI: 10.18388/abp.2020_6066

Yang Li, Chen Shan, Bo Yang, Hu Wang

{"title":"Up-regulation of HSPA1A and HSPA1B in the blood of tophi patients and its clinical significance.","authors":"Yang Li, Chen Shan, Bo Yang, Hu Wang","doi":"10.18388/abp.2020_6066","DOIUrl":"https://doi.org/10.18388/abp.2020_6066","url":null,"abstract":"Objective: Poorly treated gout can cause tophi, which can lead to serious and potentially fatal complications. This study aimed to find the potential diagnostic value of blood levels of HSPA1A and HSPA1B for tophi patients.Methods: 58 tophi patients and 61 healthy controls were enrolled in this study, and the whole venous blood samples of all subjects were collected for microarray analysis to identify differentially expressed genes associated with tophi. Meanwhile, KEGG and GO analysis were used to filtrate the enriched different expression genes. The mRNA expression levels of HSPA1A, as well as HSPA1B, were measured by the RT-qPCR method, the correlation between which and the severity of the disease were analyzed. Finally, the receiver operating characteristics curve (ROC) analysis has been performed to the diagnostic value of HSPA1A as well as HSPA1B.Results: Bioinformatic analysis results suggested that both HSPA1A and HSPA1B are abnormally expressed in tophi. Then, it was observed that HSPA1A and HSPA1B were dramatically increased in the blood samples of tophi patients compared with healthy controls and were further linked with the severity of tophi. Moreover, the area under the curve (AUC) of HSPA1A for the diagnosis of ACI was 0.8999 (95% confidence interval (CI), 0.8338 to 0.9661) while of HSPA1B was 0.9093 (95% confidence interval (CI), 0.8550 to 0.9635), suggesting that blood level of HSPA1A, as well as HSPA1B, are sensitive markers to distinguish tophi patients from the healthy people.Conclusion: HSPA1A and HSPA1B were over-expressed in the blood of tophi patients and may be potential diagnostic markers for tophi.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"69 4","pages":"781-785"},"PeriodicalIF":1.7,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10399180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Club cell secretory protein 16 is a potential biomarker for silica-induced pulmonary fibrosis. 俱乐部细胞分泌蛋白16是二氧化硅诱导肺纤维化的潜在生物标志物。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2022-12-14 DOI: 10.18388/abp.2020_5669

Lei Yang, Hua Wang, Ming Liu, Chenguang Wang, Yuehua Zuo, Ziqin Zhai

{"title":"Club cell secretory protein 16 is a potential biomarker for silica-induced pulmonary fibrosis.","authors":"Lei Yang, Hua Wang, Ming Liu, Chenguang Wang, Yuehua Zuo, Ziqin Zhai","doi":"10.18388/abp.2020_5669","DOIUrl":"https://doi.org/10.18388/abp.2020_5669","url":null,"abstract":"This study was conducted to investigate the changes of Club cell protein 16 (CC16) and surfactant protein D (SP-D) levels in serum and bronchoalveolar lavage fluid (BALF) in silicotic rats and to explore their potential as early biomarkers for silicosis. Pulmonary fibrosis models of rats were constructed by exposing them to silica particles. BALF and serum were collected to determine CC16 and SP-D levels using enzyme-linked immunosorbent assay (ELISA) at different times after the exposure. Hydroxyproline (HYP) level in BALF and CC16 level in the lung tissues were also measured immunohistochemistrially. The BALF levels of CC16 decreased from 49.65 to 38.02 ng/mg after the rats were exposed to silica for 3 and 28 days, which were all significantly lower as compared with the controls (P<0.05), where the levels remained barely changed during the same period (61.27 to 56.76 ng/mg). The serum CC16 also showed a similar decrease from 9.8 ng/ml to 8.78 ng/ml during the period, while in the controls, the serum CC16 levels remained constantly between 11.04 and 10.96 ng/ml. The levels of SP-D in the serum of silica-exposed rats did not decrease as compared with the controls and BALF SP-D presented a parabolic curve change with silica exposure. Immunohistochemical examinations showed that the lung Club cells were severely damaged and CC16 expression was obviously decreased after silica exposure. BALF HYP level was higher in silica-exposed rats than in control only when the exposure was at 50 mg/ml. Our work demonstrates that expressions of CC16 and SP-D are pulmonary tissue-specific and CC16 expression is down-regulated as a result of silica-exposure. The significant relationship between CC16 and silica dose indicates that CC16 may be exploited as an early biomarker to assess silica-induced pulmonary fibrosis.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"69 4","pages":"697-702"},"PeriodicalIF":1.7,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10445407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal miR-655-3p inhibits growth, and invasion and macrophage M2 polarization through targeting CXCR4 in papillary thyroid carcinoma. 外泌体miR-655-3p通过靶向CXCR4抑制甲状腺乳头状癌的生长、侵袭和巨噬细胞M2极化。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2022-12-13 DOI: 10.18388/abp.2020_6027

Lei Qiao, Chao Dong, Wenlei Jia, Binlin Ma

{"title":"Exosomal miR-655-3p inhibits growth, and invasion and macrophage M2 polarization through targeting CXCR4 in papillary thyroid carcinoma.","authors":"Lei Qiao, Chao Dong, Wenlei Jia, Binlin Ma","doi":"10.18388/abp.2020_6027","DOIUrl":"https://doi.org/10.18388/abp.2020_6027","url":null,"abstract":"Papillary thyroid cancer (PTC) is an endocrine malignancy whose incidence has increased rapidly worldwide. Exosome-miR-655-3p was down-regulated in patients with PTC. However, the effect and molecular mechanism of exosome-miR-655-3p in PTC was indistinct until now. Our study found that exosome-miR-655-3p was decreased in serum of PTC patients. Overexpression of miR-655-3p with mimics significantly shrunk the cell viability, reduced the number of chemotactic and invasive PTC cells. Besides, the proportion of CD163 positive cells and the expression of markers of M2 subtype macrophages was markedly decreased when mononuclear macrophage THP-1 was cultured with exosomes of miR-655-3p mimics. Oppositely, the inhibitor of miR-655-3p exacerbated growth, chemotaxis and invasion of PTC cells, and enhanced the M2 subtype macrophages. Structurally, miR-655-3p could target the 3' untranslated region (3'UTR) of CXCR4 and restrict the expression of CXCR4. In Xenograft tumor experiment, upregulated exosome-miR-655-3p effectively inhibited the growth of tumor and reduced the expression of CXCR4, Ki67 and CD163 in vivo. In summary, exosomal miR-655-3p inhibited growth, invasion and macrophage M2 polarization through targeting CXCR4 in papillary thyroid carcinoma. Regulating exosome-miR-655-3p/CXCR4 may be a potential treatment strategy for PTC.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"69 4","pages":"773-779"},"PeriodicalIF":1.7,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10734171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Chlorogenic acid regulates the proliferation and migration of high-grade serous ovarian cancer cells through modulating the miR199a5p/DDR1 axis. 绿原酸通过调节miR199a5p/DDR1轴调控高级别浆液性卵巢癌细胞的增殖和迁移。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2022-12-12 DOI: 10.18388/abp.2020_6381

Wang Li, Zhou Ping, Gao Xuemei, Meng Hongjuan, He Yi, Liu Xiaoli, Zhu Zhongxiang

{"title":"Chlorogenic acid regulates the proliferation and migration of high-grade serous ovarian cancer cells through modulating the miR199a5p/DDR1 axis.","authors":"Wang Li, Zhou Ping, Gao Xuemei, Meng Hongjuan, He Yi, Liu Xiaoli, Zhu Zhongxiang","doi":"10.18388/abp.2020_6381","DOIUrl":"https://doi.org/10.18388/abp.2020_6381","url":null,"abstract":"This study aimed to demonstrate that chlorogenic acid (CGA) has anticancer effects against ovarian cancer. The MTT assay was used to assess the optimum concentrations of CGA on the ovarian cancer cell lines OVCA433 and SKOV3, followed by the rate of apoptosis using Annexin V-FITC/PI. The mitochondrial membrane potential of ovarian tumour cells treated with CGA was evaluated using mitochondrial staining kits followed by Western blot analysis, immunofluorescence, and RT-PCR assays. The Trans-well migration assay conducted the percentage of cell migration, followed by wound healing and colony formation assays. CGA induces activation of mitochondria-mediated intrinsic apoptotic pathways in ovarian cancer cells. The discovery that miR-199a-5p is inversely correlated to DDR1, a receptor tyrosine kinase involved in collagen synthesis, was the major consequence of examining the various mechanisms involved in the development of ovarian cancer. After treatment with CGA, cells derived from ovarian cancer cells were deregulated partially via the miR199a5p/DDR1 axis, significantly affecting tumour suppression. DDR1 has been identified as a direct target of miR199a5p in these ovarian cancer cells. We found that CGA-induced loss of DDR1 caused the inactivation of NF-κB signalling downstream in the MMP, migration, and EMT pathways. The study results showed that CGA is a promising drug candidate for treating ovarian cancer, particularly because it exhibits anti-invasive and migrastatic properties.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"69 4","pages":"855-864"},"PeriodicalIF":1.7,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10385692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1