Acta biochimica Polonica最新文献_第10页

Involvement of Rictor/mTORC2/Akt/GLUT4 pathway in the regulation of energy metabolism in the gastric smooth muscle of diabetic rats. Rictor/mTORC2/Akt/GLUT4通路参与糖尿病大鼠胃平滑肌能量代谢的调控

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-12 DOI: 10.18388/abp.2020_5652

Sun Yan, Yu-Rong Zheng, Zheng Jin, Mo-Han Zhang, Xiang-Shu Cui

{"title":"Involvement of Rictor/mTORC2/Akt/GLUT4 pathway in the regulation of energy metabolism in the gastric smooth muscle of diabetic rats.","authors":"Sun Yan, Yu-Rong Zheng, Zheng Jin, Mo-Han Zhang, Xiang-Shu Cui","doi":"10.18388/abp.2020_5652","DOIUrl":"https://doi.org/10.18388/abp.2020_5652","url":null,"abstract":"Diabetes mellitus can be accompanied by a variety of complications. The purpose of the present study was to characterize the Rictor/mTOR complex 2 (mTORC2)/Akt/glucose transporter 4 (GLUT4) pathway and its effects on energy metabolism in the gastric smooth muscle of diabetic rats. Diabetes was induced in rats using streptozotocin and their phenotype was compared with untreated rats. The relationship between gastric motility and energy metabolism was analyzed by comparing the contraction and ATP metabolism of muscle strips. Western blotting was used to detect the expression of key proteins in the pathway. The diabetic rats demonstrated less frequent and less powerful gastric smooth muscle contractions. The concentrations of ADP, AMP, and ATP, and the energy charge in gastric smooth muscle changed in different periods of diabetes, and these changes were consistent with changes in mechanistic target of rapamycin (mTOR) protein content. The expression of the key intermediates in signal transduction in the Rictor/mTORC2/Akt/GLUT4 pathway also underwent significant changes. Rictor protein expression increased during the development of diabetes, but the activation of mTORC2 did not increase with the increase in Rictor expression. GLUT4 translocation is regulated by Akt and its expression change during the development of diabetes. These findings suggest that altered energy metabolism is present in gastric smooth muscle that is associated with changes in the Rictor/mTORC2/Akt/GLUT4 pathway. Rictor/mTORC2/Akt/GLUT4 pathway may be involved in the regulation of energy metabolism in the gastric smooth muscle of diabetic rats and the development of diabetic gastroparesis.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"233-238"},"PeriodicalIF":1.7,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9658903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of USP1 deubiquitinase in the pathogenesis and therapy of cancer. USP1去泛素酶在癌症发病和治疗中的作用。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-12 DOI: 10.18388/abp.2020_6636

Svitlana Antonenko, Michael Zavelevich, Gennady Telegeev

引用次数: 0

WIF1 was downregulated in cervical cancer due to promoter methylation. 在宫颈癌中，由于启动子甲基化，WIF1下调。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-12 DOI: 10.18388/abp.2020_6700

Ying Wang, Shifa Yuan, Jing Ma, Hong Liu, Lizhen Huang, Fengzhen Zhang, Xiaomei Wang

{"title":"WIF1 was downregulated in cervical cancer due to promoter methylation.","authors":"Ying Wang, Shifa Yuan, Jing Ma, Hong Liu, Lizhen Huang, Fengzhen Zhang, Xiaomei Wang","doi":"10.18388/abp.2020_6700","DOIUrl":"https://doi.org/10.18388/abp.2020_6700","url":null,"abstract":"Wnt inhibitory factor 1 (WIF1) is frequently downregulated in a variety of cancer due to promoter methylation. However, the methylation status of the WIF1 promoter in cervical cancer remains unclear. This study aimed to elucidate the mechanism by which WIF1 promoter methylation contributes to cervical cancer development. The expression of WIF1 in cervical cancer tissues was examined by immunohistochemistry. The methylation status of the WIF1 promoter in cervical cancer cells was detected by methylation specific PCR. WIF1 mRNA levels and protein levels were detected by PCR and Western blot analysis. We found that WIF1 expression was low in cervical cancer tissues compared to adjacent normal cervical tissues. The WIF1 promoter was methylated in the cervical cancer SiHa cell line but not in the normal cervical epithelial cell line Ect1. Correspondingly, WIF1 mRNA levels and protein levels were significantly lower in SiHa cells than in Ect1 cells. Treatment with 5-aza-2-deoxycytidine (AZA) led to the upregulation of WIF1 mRNA and protein levels in SiHa cells, but the effects were abrogated by treatment with WIF1 siRNA. In addition, AZA treatment induced apoptosis and inhibited the invasion of SiHa cells, and the effects were abrogated by WIF1 siRNA. The protein levels of survivin, c-myc and cyclinD1 were significantly lower in SiHa cells treated with AZA, but their levels were upregulated after treatment with WIF1 siRNA. In conclusion, the methylation of the WIF1 promoter leads to the downregulation of WIF1 and the activation of Wnt/β-catenin signaling in cervical cancer cells. WIF1 is a tumor suppressor that is inactivated in cervical cancer.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"419-423"},"PeriodicalIF":1.7,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoplasmic reticulum stress involves the high glucose-induced nucleus pulposus cell pyroptosis. 内质网应激涉及高糖诱导的髓核细胞焦亡。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-12 DOI: 10.18388/abp.2020_6602

Xiaochun Xiong, Ying Wu, Hengguo Long, Tianzi Liang, Wenqing Liang, Xiaogang Huang, Guijin Li

{"title":"Endoplasmic reticulum stress involves the high glucose-induced nucleus pulposus cell pyroptosis.","authors":"Xiaochun Xiong, Ying Wu, Hengguo Long, Tianzi Liang, Wenqing Liang, Xiaogang Huang, Guijin Li","doi":"10.18388/abp.2020_6602","DOIUrl":"https://doi.org/10.18388/abp.2020_6602","url":null,"abstract":"Objective: Diabetes has been identified as a risk factor for intervertebral disc degeneration (IDD). The aim of this study is to investigate the potential mechanism underlying diabetes-related pyroptosis in nucleus pulposus (NP) cells.Methods: We used a high-glucose environment to mimic diabetes in vitro and examined the endoplasmic reticulum stress (ERS) and pyroptotic response. Furthermore, we utilized activators and inducers of ERS to explore the role of ERS in high-glucose-induced pyroptosis in NP cells. We evaluated the ERS and pyroptosis levels using immunofluorescence (IF) or RT-PCR and measured the expression of collagen II, aggrecan, and MMPs. Additionally, we used ELISA to determine the levels of IL-1β and IL-18 in the culture medium, and CCK8 assay to test cell viability.Results: High-glucose conditions promoted the degeneration of NP cells and triggered ERS and pyroptosis. A high level of ERS aggravated pyroptosis, and partially suppressing ERS resisted high-glucose-induced pyroptosis and alleviated the degeneration of NP cells. Inhibiting caspase-1-based pyroptosis under high-glucose conditions helped relieve the degeneration of NP cells but did not affect ERS levels.Conclusions: High-glucose induces pyroptosis in NP cells via the mediation of ERS, and suppressing ERS or pyroptosis protects NP cells under high-glucose conditions.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"371-377"},"PeriodicalIF":1.7,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9652138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Overexpression of long non-coding RNA GASL1 induces apoptosis and G0/G1 cell cycle arrest in human oral cancer cells. 过表达长链非编码RNA GASL1可诱导人口腔癌细胞凋亡和G0/G1细胞周期阻滞。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-12 DOI: 10.18388/abp.2020_6473

Rui Zhang, Wanjun Tao, Lei Yu

{"title":"Overexpression of long non-coding RNA GASL1 induces apoptosis and G0/G1 cell cycle arrest in human oral cancer cells.","authors":"Rui Zhang, Wanjun Tao, Lei Yu","doi":"10.18388/abp.2020_6473","DOIUrl":"https://doi.org/10.18388/abp.2020_6473","url":null,"abstract":"Oral cancer is one of the commonly reported malignancies of the human oral cavity and pharynx. It accounts for a significant level of cancer-based mortality across the globe. Long non-coding RNAs (lncRNAs) are emerging as important study targets in cancer therapy. The present study aimed to characterize the role of lncRNA GASL1 in regulating the growth, migration, and invasion of human oral cancer cells. The qRT-PCR showed significant (P<0.05) upregulation of GASL1 in oral cancer cells. Overexpression of GASL1 led to the loss of viability of HN6 oral cancer cells by inducing apoptosis which was associated with upregulation of Bax and downregulation of Bcl-2. The apoptotic cell percentage increased from 2. 81% in control to 25.89% upon GASL1 overexpression. Cell cycle analysis showed that overexpression of GASL1 increased the G1 cells from 35.19% in control to 84.52% upon GASL1 overexpression indicative of G0/G1 cell cycle arrest. Cell cycle arrest was also accompanied by inhibition of cyclin D1 and CDK4 protein expression. Wound healing and transwell assays showed that overexpression of GASL1 significantly (P<0.05) inhibited the migration and invasion of HN6 oral cancer cells. The invasion of the HN6 oral cancer cells was found to be decreased by more than 70%. Finally, the results of in vivo study revealed that GASL1 overexpression inhibits the xenografted tumor growth in vivo. Thus, the results are thus suggestive of the tumor-suppressive molecular role of GASL1 in oral cancer cells.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"271-276"},"PeriodicalIF":1.7,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9658907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Were academic promotions in biochemistry and other research disciplines improperly controlled in Poland between 2011 and 2020? A response to the recently published "Who controls the national academic promotion system" article. 2011年至2020年期间，波兰生物化学和其他研究学科的学术晋升是否受到不当控制?对最近发表的“谁控制了国家学术晋升体系”文章的回应。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-07 DOI: 10.18388/abp.2020_6873

Grzegorz Węgrzyn

{"title":"Were academic promotions in biochemistry and other research disciplines improperly controlled in Poland between 2011 and 2020? A response to the recently published \"Who controls the national academic promotion system\" article.","authors":"Grzegorz Węgrzyn","doi":"10.18388/abp.2020_6873","DOIUrl":"https://doi.org/10.18388/abp.2020_6873","url":null,"abstract":"In the recently published article by Koza et al. (SAGE Open, 2023, 13, doi: 10.1177/21582440231177974), the authors analyzed the academic promotion system in Poland between 2011 and 2020. They concluded that \"the Polish system of academic promotions in the past decade can hardly be regarded as based on pure merit\", suggesting the impropriety, based on the participance of the members of the Central Board for Degrees and Titles in panels of experts evaluating the applications. Biochemistry was provided as a research discipline in which such an \"impropriety\" was the most pronounced, though other disciplines were only slightly less \"improperly affected\". Although the calculations presented by Koza and others (Koza et al., 2023) were proper, their conclusions were affected by fundamental errors in assessing the roles of the panelists and misinterpretation of the data. The drawbacks of the interpretations of the facts and in drawing conclusions are presented and discussed in this paper, underlining the necessity of being very careful when assessing any phenomenon and concluding about any mechanism. Indeed, only very well substantiated conclusions, strongly supported by objective data, should be published. This rule is very well known in biochemistry and other exact and natural sciences, and should be mandatory in all other research disciplines.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"465-467"},"PeriodicalIF":1.7,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of 6-hydroxydopamine increase the glutathione level in SH-SY5Y human neuroblastoma cells. 6-羟多巴胺增加SH-SY5Y人神经母细胞瘤细胞谷胱甘肽水平的作用。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-06-01 DOI: 10.18388/abp.2020_6847

Natalia Pieńkowska, Grzegorz Bartosz, Izabela Sadowska-Bartosz

{"title":"Effect of 6-hydroxydopamine increase the glutathione level in SH-SY5Y human neuroblastoma cells.","authors":"Natalia Pieńkowska, Grzegorz Bartosz, Izabela Sadowska-Bartosz","doi":"10.18388/abp.2020_6847","DOIUrl":"https://doi.org/10.18388/abp.2020_6847","url":null,"abstract":"Treatment of human neuroblastoma SH-SY5Y cells with a catecholaminergic neurotoxin, 6-hydroxydopamine (6-OHDA) is an acknowledged in vitro experimental model of Parkinson disease (PD). A decrease in the glutathione content occurs in PD. Higher concentrations of 6-OHDA lowered the glutathione level in SH-SY5Y cells, nonetheless, we and other authors found a considerable increase in these cells' glutathione content after 24 h treatment with 60 μM 6-OHDA. A synthetic antioxidant, 4-aminotetramethylpiperidine-1-oxyl (4-AT) exerted a similar effect. The aim of the present study was to explain this surprising effect by monitoring the time course of changes in the levels of reduced (GSH) and oxidized glutathione (GSSG), total antioxidant activity (TAC) of human neuroblastoma cell SH-SY5Y extracts as well as the level of reactive oxygen species and activities of enzymes of glutathione metabolism after treatment of the cells with 60 µM 6-OHDA and/or 4-AT for 30 min - 24 h. A transient decrease in the level of GSH and TAC of cell extracts, increase in the level of GSSG, and decrease in the activities of glutathione peroxidase, glutathione reductase, glutathione S-transferase and γ-glutamyl-cysteine ligase activities were found followed by normalization or overshoot of the GSH level, TAC and enzyme activities. Increased activity of γ-glutamyl-cysteine ligase activity starting after 4-6 h was responsible for the elevation of the level of GSH and TAC in cells treated with 6-OHDA, 4-AT, and both compounds. The 6-OHDA-induced increase in the GSH content is a result of an overcompensatory response. The antioxidant 4-AT may be useful for the induction of an increase in the level of GSH in neural cells, without the negative effect of 6-OHDA.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"457-464"},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of poor nutrition status in multiple sclerosis patients assessed by different diagnostic tools. 不同诊断工具评估多发性硬化症患者营养不良状况的患病率。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-05-31 DOI: 10.18388/abp.2020_6578

Natalia Mogiłko, Sylwia Malgorzewicz

{"title":"Prevalence of poor nutrition status in multiple sclerosis patients assessed by different diagnostic tools.","authors":"Natalia Mogiłko, Sylwia Malgorzewicz","doi":"10.18388/abp.2020_6578","DOIUrl":"https://doi.org/10.18388/abp.2020_6578","url":null,"abstract":"Patients with multiple sclerosis (MS) present a spectrum of nutritional disorders from obesity to malnutrition. The purpose of this study was an assessment of the nutritional status of MS patients by NRS-2002 and GLIM criteria.Methods: 147 patients were included in the study. The nutritional status was assessed by NRS 2002, GLIM, and body composition analysis. The routine biochemical parameters were measured.Results: Deterioration of the nutritional status was observed in 87.8% of patients. GLIM criteria indicated that 20% of patients were malnourished and 80% were at risk. The percentage of patients with excess body mass was 46.8%, and of underweight patients was 6.6%. The risk of malnutrition was positively associated with low content of adipose tissue (R=-0.24; p=0.00), low BMI (R=-0.22; p=0.00), and higher weight loss in the last 6 months (R=0.47; p=0.00). Additionally, a significant (p<0.05) correlation between malnutrition state and s-albumin (R=-0.2) and CRP (R=0.23) was observed.Conclusion: Overweight and obesity concerned a large proportion of the studied group of MS patients, but this does not exclude the risk of malnutrition. Dietary care and regular outpatient nutritional status assessment should be provided throughout the disease.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"343-345"},"PeriodicalIF":1.7,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of indigenous bacterial assemblage for biodegradation of persistent herbicides in the soil. 土壤中生物降解持久性除草剂的本地细菌组合的分离和特性。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-05-31 DOI: 10.18388/abp.2020_6563

Mariam Zameer, Usaal Tahir, Sana Khalid, Nureen Zahra, Abid Sarwar, Tariq Aziz, Ahsan Saidal, Majid Alhomrani, Abdulhakeem S Alamri, Anas S Dablool, Manal Y Sameeh, Amal A Mohamed, Amnah Alharbi

{"title":"Isolation and characterization of indigenous bacterial assemblage for biodegradation of persistent herbicides in the soil.","authors":"Mariam Zameer, Usaal Tahir, Sana Khalid, Nureen Zahra, Abid Sarwar, Tariq Aziz, Ahsan Saidal, Majid Alhomrani, Abdulhakeem S Alamri, Anas S Dablool, Manal Y Sameeh, Amal A Mohamed, Amnah Alharbi","doi":"10.18388/abp.2020_6563","DOIUrl":"https://doi.org/10.18388/abp.2020_6563","url":null,"abstract":"Extensive pesticides (herbicides) use is negatively disturbing the environment and humans. Pesticide bioremediation with eco-friendly techniques bears prime importance. This study aimed to isolate and characterize three different herbicides (metribuzin, clodinafop- propargyl, MCPA (2-methyl, 4 chlorophenoxyacetic acids) and Bromoxynil) degrading bacterial strains from agricultural fields of Punjab University, Pakistan. Among the 12 bacterial isolates, 5 were metribuzin degrading, 3 were clodinafop propargyl degrading and, 4 were MCPA and Bromoxynil degrading bacteria. Morphological, microscopic, and molecular characterization revealed that the majority of these bacterial strains were gram-negative and belonged to Bacillus and Pseudomonas genera. The isolates A6, B3, and C1 were subjected to respective herbicide degradation and the data was confirmed through GC-MS analysis. The effect of herbicide concentrations, pH, and temperature on bacterial growth was determined at OD600. The strain A6 degraded 14.8% metribuzin out of the provided concentration of 50 ppm by following the deamination pathway. While the isolates B3 and C1 degraded 23.2% and 33.9% clodinafop, MCPA and bromo-xynil, respectively, at a spiking concentration of 50ppm. The clodinafop, MCPA and Bromoxynil were metabolized into less toxic products i.e., dicarboxylic acids and 2-methyl phenol respectively, and metabolized via decarboxylation and dehalogenation mechanism. The present study evaluates the herbicides degrading bacterial strains that could potentially be used for bioremediation of agricultural contaminated sites.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"325-334"},"PeriodicalIF":1.7,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MiRNA-19b-3p downregulates the endothelin B receptor in gastric cancer cells to prevent angiogenesis and proliferation. MiRNA-19b-3p下调胃癌细胞内皮素B受体抑制血管生成和增殖。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-05-25 DOI: 10.18388/abp.2020_6595

Xiaodong Hu, Hongbing Liu, Chaoyu Li

{"title":"MiRNA-19b-3p downregulates the endothelin B receptor in gastric cancer cells to prevent angiogenesis and proliferation.","authors":"Xiaodong Hu, Hongbing Liu, Chaoyu Li","doi":"10.18388/abp.2020_6595","DOIUrl":"https://doi.org/10.18388/abp.2020_6595","url":null,"abstract":"MiR19b-3p acts as a tumor suppressor gene in various cancers, but its function in gastric cancer is unknown. This study investigated the role of miR19b-3p in angiogenesis and the proliferation of human gastric cancer cells targeting ETBR expression. Cell proliferation in SGC-7901 cells, cell transfection, luciferase reporter assay, detection of endothelin B receptor mRNAs by RT-qPCR, and a Western blot assay were carried out. RT-qPCR expression analysis showed a prominent (p<0.01) downregulation of miR19b-3p in SGC-7901 cells, which was inversely correlated with a substantial increase (p<0.01) in the endothelin B receptor (ETBR). However, overexpression of miR19b-3p in SGC-7901 cells with its mimic (p<0.01) resulted in the loss of cell viability in the MTT assay. This effect was reversed (p<0.01) by the inhibitor. Western blot analysis revealed that ETBR was significantly (p<0.01) decreased by miR19b-3p overexpression compared with that of the negative control or its inhibitor. Based on bioinformatics tools and luciferase reporter assays, we found that miR19b-3p interacts with the 3'untranslated region (3'UTR) of ETBR. Restoring miR19b-3p overexpression with its mimic led to downregulation of ETBR in gastric cancer cells (SGC-7901), which significantly (p<0.01) decreased the expression of vascular endothelial growth factor A (VEGF-A). These findings were considerably reversed by miR19b-3p inhibitors (p<0.01). The results indicated that miR19b-3p exerts its molecular action by targeting ETBR at the post-transcriptional level by regulating angiogenesis and proliferation by overexpressing miR19b-3p as a potential treatment target for gastric cancer.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 2","pages":"363-370"},"PeriodicalIF":1.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0