Circ-POLA2介导的miR-138-5p/SEMA4C轴影响结肠癌细胞的活性。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Acta biochimica Polonica Pub Date : 2023-08-17 DOI:10.18388/abp.2020_6457

YanDong Huang, QingYang Bai, HongBo Yu, YanRu Li, Hao Lu, HuiMin Kang, XueWei Shi, Kai Feng

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引用次数: 0

摘要

本研究旨在探讨circ-POLA2在癌症（CC）中的作用机制。记录CC组织和细胞中的Circ-POLA2、miR-138-5p和SEMA4C水平。测定了circ-POLA2、miR-138-5p或SEMA4C介导的对细胞增殖、迁移、侵袭和凋亡的影响。对circ-POLA2/miR-138-5p/SEMA4C的反馈回路进行了调查。据测量，circ-POLA2和SEMA4C高表达，而miR-138-5p低表达。同时，circ-POLA2可以通过miR-138-5p靶向介导SEMA4C。Circ-POLA2敲低导致细胞活性阻断，但miR-138-5p抑制或SEMA4C过表达减轻了这种作用。总之，circ-POLA2通过miR-138-5p/SEMA4C轴对CC具有致瘤性，这可能为CC治疗提供一个有前景的分子靶点。

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Circ-POLA2-mediated miR-138-5p/SEMA4C axis affects colon cancer cell activities.

This study aimed to investigate the mechanism of circ-POLA2 in colon cancer (CC). Circ-POLA2, miR-138-5p, and SEMA4C levels in CC tissues and cells were recorded. The influences mediated by circ-POLA2, miR-138-5p or SEMA4C on cell proliferation, migration, invasion, and apoptosis were determined. The feedback loop of circ-POLA2/miR-138-5p/SEMA4C was surveyed. As measured, circ-POLA2 and SEMA4C were highly expressed, while miR-138-5p was poorly expressed. Meanwhile, circ-POLA2 could mediate SEMA4C through miR-138-5p targeting. Circ-POLA2 knockdown caused the blockade for cell activities, but this effect was alleviated by miR-138-5p inhibition or SEMA4C overexpression. Overall, circ-POLA2 is tumorigenic for CC through miR-138-5p/SEMA4C axis, which may provide a promising molecular target for CC therapy.

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来源期刊

Acta biochimica Polonica 生物-生化与分子生物学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Acta Biochimica Polonica is a journal covering enzymology and metabolism, membranes and bioenergetics, gene structure and expression, protein, nucleic acid and carbohydrate structure and metabolism.