Acta biochimica Polonica最新文献

{"title":"Mitochondrial respiration and nucleotide profiling in human left internal mammary artery from CABG: a novel real-time <i>ex vivo</i> approach.","authors":"Alicja Braczko, Andrzej Łoś, Marta Piotrowska, Ada Kawecka, Iga Walczak, Mikołaj Krysiak, Marcin Hellmann, Maciej Brzeziński, Ryszard T Smoleński, Barbara Kutryb-Zając","doi":"10.3389/abp.2026.16877","DOIUrl":"10.3389/abp.2026.16877","url":null,"abstract":"<p><p>Mitochondrial dysfunction plays a critical role in the pathogenesis of cardiovascular and metabolic diseases. However, direct assessment of mitochondrial respiration in human vascular tissue remains technically challenging. In this study, we present an <i>ex vivo</i> approach for real-time analysis of mitochondrial respiration in human left internal mammary artery (LIMA) grafts obtained during coronary artery bypass grafting (CABG). LIMA segment was collected intraoperatively and processed for bioenergetic assessment using the Seahorse XF Flex 3D Analyzer. Mitochondrial respiration was evaluated using the Mito Stress Test, enabling real-time measurement of oxygen consumption rate (OCR). In parallel, tissue nucleotide levels were quantified using high-performance liquid chromatography (HPLC), allowing complementary assessment of cellular energy status and redox balance. We demonstrate the feasibility of measuring mitochondrial respiration in intact human arterial tissue <i>ex vivo</i>. To our knowledge, this is the first application of the Seahorse XF Flex 3D platform for real-time bioenergetic analysis in intact human vascular tissue. Combined analysis of OCR and nucleotide levels enabled integrated assessment of vascular bioenergetic status. The applied protocol enabled reliable assessment of key bioenergetic parameters, including basal and maximal respiration. This study establishes a novel proof-of-concept workflow for <i>ex vivo</i> bioenergetic profiling of human vascular grafts, providing a platform for future investigations of vascular metabolism in cardiovascular and metabolic disorders.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16877"},"PeriodicalIF":2.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13453951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148704802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of gut microbiome disruption in the development of metabolic dysfunction-associated kidney disease.","authors":"Michał Witkowski, Jarosław Przybyciński, Bartosz Wojciuk, Wiktor Czaja, Natalia Gołembiewska, Edyta Gołembiewska","doi":"10.3389/abp.2026.16436","DOIUrl":"10.3389/abp.2026.16436","url":null,"abstract":"<p><p>Metabolic dysfunction-associated kidney disease (MDAKD) is increasingly recognised as a major clinical consequence of the global rise in obesity, type 2 diabetes, hypertension, and cardiovascular disease. Accumulating evidence suggests that the gut microbiota may contribute to the development and progression of metabolic and renal disorders through complex mechanisms involving microbial metabolites, immune activation, and disruption of the intestinal barrier. This review provides an overview of current knowledge regarding the role of the gut microbiota and gut-derived metabolites in the pathogenesis of chronic kidney disease (CKD) associated with metabolic disorders. Special attention is given to short-chain fatty acids, bile acids, N-trimethylamine oxide, branched-chain amino acids, indoxyl sulfate, p-cresol sulfate, and lipopolysaccharides. Accumulating experimental and clinical evidence suggests that dysbiosis may contribute to chronic low-grade inflammation, insulin resistance, endothelial dysfunction, lipotoxicity, and profibrotic signaling pathways associated with kidney injury and cardiovascular complications. The review also identifies significant limitations in current microbiome research, such as the predominance of animal studies, methodological challenges in metabolite quantification, and difficulties in establishing causality in humans. Emerging therapeutic strategies targeting the gut microbiota, including dietary interventions, prebiotics, probiotics, sodium-glucose cotransporter 2 inhibitors, glucagon-like peptide-1 receptor agonists, and faecal microbiota transplantation, may offer novel approaches to slowing CKD progression and improving metabolic health. However, further mechanistic and clinical studies are required to determine the efficacy of microbiota-targeted therapies in MDAKD.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16436"},"PeriodicalIF":2.2,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148688345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening performance of the MUS-3600 urine analyzer for urinary tract infection: comparison with urine culture.","authors":"Zeynep Mine Yalçınkaya Kara, Leyla Genç, Merve Sena Odabaşı, Elif Aktaş","doi":"10.3389/abp.2026.16555","DOIUrl":"10.3389/abp.2026.16555","url":null,"abstract":"<p><strong>Background: </strong>Urine culture is the gold standard for diagnosing urinary tract infections (UTIs), but most samples yield negative results. A rapid and accurate screening method could accelerate reporting, reduce workload, and lower costs. This study aimed to evaluate the diagnostic performance of the MUS-3600 urine analyzer as a screening tool for UTIs.</p><p><strong>Methods: </strong>Urine samples submitted to the microbiology laboratory were analyzed using the MUS-3600 within 10 min of collection. The analyzer uses flow-type micro-imaging and artificial intelligence-based particle classification. Culture results with ≥10⁴ CFU/mL were defined as positive; lower counts were negative.</p><p><strong>Results: </strong>For white blood cells (WBC, >39/μL), sensitivity, specificity, PPV, NPV, and elimination rate were 63.4%, 84.5%, 50.8%, 90.1%, and 67.5%, respectively. Bacilli (>103/μL) showed 62.1%, 88.1%, 56.9%, 90.2%, and 70.4%. Suspected cocci (>859/μL) demonstrated lower accuracy (44.4%, 78.7%, 34.5%, 84.9%, and 62.8%). Combining WBC and bacilli yielded the highest elimination rate (77.2%) and NPV (87.9%). The analyzer performed better in males (NPV 93.9%) than in females (NPV 82.1%).</p><p><strong>Conclusion: </strong>The MUS-3600 provides moderate accuracy for UTI screening and may help reduce unnecessary urine cultures, particularly in male patients. However, its limited sensitivity, especially for cocci, prevents its use as a stand-alone screening tool. Further studies are needed to confirm its role as a complementary method for culture elimination.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16555"},"PeriodicalIF":2.2,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13422189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond biochemistry: multiparametric ultrasound parameters and their molecular correlates in cardio-renal-metabolic syndrome.","authors":"Marcin L Kutek, Jacek M Witkowski","doi":"10.3389/abp.2026.16873","DOIUrl":"10.3389/abp.2026.16873","url":null,"abstract":"<p><p>Cardio-renal-metabolic syndrome (CRMS)-characterized by the pathological interplay of visceral adiposity, insulin resistance, chronic kidney disease, and cardiovascular disease-affects over 90% of US adults across its staging spectrum, yet its multi-organ burden remains difficult to assess non-invasively at the point of care. This narrative mini review examines whether multiparametric ultrasound within a single examination can serve as an integrated imaging biomarker set complementary to established molecular markers of CRMS. A narrative search of PubMed (2015-2026) was conducted using PICO-structured queries. The hepatic controlled attenuation parameter and liver stiffness measurement correlate directly with Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), CRP, and adipokine dysregulation, with pooled CAP cutoffs of 268-313 dB/m and elastography thresholds of 8.2-13.6 kPa for fibrosis staging in MASLD. Epicardial adipose tissue thickness correlates with circulating IL-17A (r = 0.308), hs-CRP (r = 0.666), and HOMA-IR (r = 0.567-0.580), independently predicting left ventricular diastolic dysfunction beyond BMI. Carotid intima-media thickness tracks eGFR decline longitudinally and predicts cardiovascular mortality in CKD populations. Renal resistive index, with a validated threshold of ≥0.70, independently predicts GFR decline and cardiovascular mortality across diabetic and hypertensive populations. Renal shear wave elastography distinguishes fibrotic from non-fibrotic parenchyma, with 93.1% sensitivity against biopsy. Cross-compartmental correlations and composite imaging-biochemistry models consistently outperform single-parameter approaches. Two critical gaps remain: specific ultrasound values have not been associated with AHA/ACC CKM stages, and no outcome study has validated a multiorgan protocol. Multiparametric ultrasound provides a clinically feasible, evidence-based CRMS assessment that remains to be validated in prospective trials.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16873"},"PeriodicalIF":2.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13407380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148597508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergence in surface protein exposure between reference and clinical-derived <i>Candida glabrata (Nakaseomyces glabratus)</i> strains (CBS138 vs. BG2) - a preliminary proteomic perspective.","authors":"Aneta Bednarek, Olga Barczyk-Woznicka, Justyna Karkowska-Kuleta, Elzbieta Pyza, Maria Rapala-Kozik, Dorota Satala","doi":"10.3389/abp.2026.16376","DOIUrl":"10.3389/abp.2026.16376","url":null,"abstract":"<p><p>Candida <i>glabrata</i> (currently classified as <i>Nakaseomyces glabratus</i>) is an opportunistic fungal pathogen notable for its intrinsic antifungal tolerance and ability to persist in host environments. Although strain CBS138 has served as the principal model for genetic and functional studies, accumulating evidence indicates substantial intraspecies diversity that may shape virulence, immune interactions and stress adaptation. In particular, the widely used clinical isolate BG2 differs from CBS138 in genome structure, adhesin regulation and macrophage survival, yet the extent to which these differences are reflected at the fungal cell surface remains unknown. Here, we present a comparative characterization of the surface-exposed proteomes (surfaceomes) of CBS138 and BG2 across three biologically relevant growth conditions: YPD-grown yeast-like cells, RPMI-cultured planktonic aggregates and RPMI-formed biofilms. Using trypsin shaving combined with LC-MS/MS, we identified pronounced strain- and condition-dependent differences in surface protein composition, encompassing adhesins, yapsin proteases and selected moonlighting proteins. Whereas CBS138 showed greater representation of adhesion- and interaction-related surface proteins, BG2 preferentially displayed proteins associated with cell-wall architecture and remodelling, consistent with distinct surface-mediated adaptive strategies. Transmission electron microscopy revealed condition-dependent differences in cell-wall thickness in both strains, with BG2 displaying a broader range of values and the highest thickness under biofilm conditions, providing structural context for variation in protease accessibility and surface-protein detectability. Collectively, our findings highlight substantial surfaceome plasticity in <i>C. glabrata</i> and underscore the importance of considering intraspecies diversity when interpreting host-pathogen interactions and fungal virulence pathways.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16376"},"PeriodicalIF":2.2,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13388231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial morphology and mtDNA content in fibroblasts from patients with different types of mucopolysaccharidosis.","authors":"Klaudia Walczak, Lidia Gaffke, Karolina Pierzynowska, Natalia Sowa-Rogozińska, Ewa Piotrowska","doi":"10.3389/abp.2026.16345","DOIUrl":"10.3389/abp.2026.16345","url":null,"abstract":"<p><p>Mucopolysaccharidosis (MPS) is a group of inherited metabolic diseases, characterized by defects in the degradation of glycosaminoglycans and their accumulation in lysosomes. However, various secondary cellular changes also contribute to the pathomechanism of MPS. Previous studies have reached contradictory conclusions about the changes in mitochondria in MPS, from increased numbers of mitochondria to impaired activities of some mitochondrial respiratory chain enzymes to no changes in mitochondrial respiration. In this preliminary, hypothesis-generating study, mitochondrial network morphology and mitochondrial DNA (mtDNA) abundance were investigated in fibroblasts derived from patients suffering from diverse MPS types. Fluorescence microscopy and real-time PCR were used to estimate these parameters, respectively. No significant changes in the mitochondrial network morphology were detected in MPS fibroblasts relative to control cells. Decreased levels of mtDNA relative to nuclear DNA levels were evident in some (I, II, IIIA, IIID, and VI) but not all MPS types compared to control fibroblasts. The results of this study suggest that there are some, although perhaps not dramatic, impairments of mitochondrial functions in some MPS types; however, they do not provide direct evidence of mitochondrial dysfunction. Therefore, these findings should be interpreted as descriptive and exploratory, highlighting the need for further functional and mechanistic studies.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16345"},"PeriodicalIF":2.2,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13333542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elemental changes in tooth hard tissues during demineralization and fluoride-induced remineralization: an SEM-EDX study.","authors":"Svanishvili Nino, Mamaladze Marina, Mauro Labanca, Vadachkoria Dea","doi":"10.3389/abp.2026.16706","DOIUrl":"10.3389/abp.2026.16706","url":null,"abstract":"<p><strong>Background: </strong>Tooth enamel and dentin are the most highly mineralized tissues in the human body, and their structural stability depends on the balanced content of calcium (Ca), phosphorus (P), and fluoride (F). Demineralization leads to the loss of these ions, weakening the tooth structure and increasing susceptibility to caries.</p><p><strong>Objective: </strong>This study aimed to evaluate elemental changes in Ca, P, and F in intact, demineralized, and remineralized teeth across the enamel, dentin-enamel junction and peripulpal dentin.</p><p><strong>Materials and methods: </strong>45 extracted human teeth were longitudinally sectioned to yield 90 paired specimens. One half of each tooth served as a baseline (intact) control, while the corresponding half was subjected to acid-induced demineralization. Following demineralization, the experimental halves were randomly assigned to one of three remineralization protocols (n = 15 per group): fluoride varnish, fluoride toothpaste with rinsing, and fluoride toothpaste without rinsing. Elemental composition was analyzed using scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX). Statistical analysis included paired t-tests for within-tooth comparisons and one-way ANOVA for treatment effects (p < 0.05).</p><p><strong>Results: </strong>At baseline, enamel exhibited significantly higher calcium and phosphorus levels compared to dentin regions. Demineralization resulted in a significant reduction in mineral content, particularly in dentin, along with complete loss of detectable fluoride (p < 0.0001). Remineralization increased calcium and fluoride levels across all groups. Fluoride varnish showed the highest fluoride retention trend, particularly in enamel; however, no statistically significant differences were observed among treatment groups for calcium and phosphorus.</p><p><strong>Conclusion: </strong>Fluoride-based treatments contributed to partial remineralization of tooth hard tissues. Fluoride varnish showed a tendency toward greater fluoride incorporation, particularly in enamel; however, statistically significant superiority over other treatment approaches was not demonstrated. These findings support the role of fluoride in remineralization while highlighting the need for further clinically relevant studies.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16706"},"PeriodicalIF":2.2,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The promise and peril of mirror bacteria.","authors":"Kyna Rigouts, Gregor Becker","doi":"10.3389/abp.2026.16673","DOIUrl":"10.3389/abp.2026.16673","url":null,"abstract":"<p><p>Mirror bacteria are organisms constructed from mirror-image biomolecules unlike those used by all known life and are moving from theoretical concept to practical feasibility. Their unusual chemistry that makes them resistant to natural degradation and isolated from ecosystems, fuels optimism for applications such as more stable medicines, long-term information storage, and novel platforms for bioengineering. At the same time, these very characteristics raise reservations: they challenge existing definitions of \"life,\" expose gaps in regulation, and risk undermining public trust if deployed without careful oversight. This perspective assesses not only those risks but also the opportunities of mirror bacteria, moving beyond narrow biosafety debates to explore their broader ethical, regulatory, and cultural implications. We argue that mirror bacteria represent a rare testcase for how science and society navigate the responsibilities of creating fundamentally new forms of life. Their development offers not only technical possibilities but also a chance to build more resilient frameworks for governance and ethics in synthetic biology.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16673"},"PeriodicalIF":2.2,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13351816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise genome modification of agrobacterium based on one-step homologous recombination via endogenous transfer DNA.","authors":"Yu Liu, Geng Sun, Chunyan Gao, Rui Gao, Jian Tang, Menghu Wang, Jian Guo, Rui Guo","doi":"10.3389/abp.2026.16599","DOIUrl":"10.3389/abp.2026.16599","url":null,"abstract":"<p><p>Here, a simple and precise genome modification method for agrobacterium was described and tested in strain GV3101. Taking the induced endogenous Transfer DNA (T-DNA) as the single-strand homologous repair template, this makes it possible to achieve valid and accurate genome modification for agrobacterium even without the strengthening of gene editing tools such as the CRISPR/Cas9 system. In addition, the introduction of tool plasmids into recipient agrobacteria can be achieved by the freeze-thaw method rather than complex triparental mating or expensive electroporation, which makes it more convenient. Here, the modification rate of agrobacterium strain GV3101 at both the upstream and downstream of VirD2 is 4.5% with 100% sequence accuracy. This research provides a valuable potential tool for genetic engineering in agrobacterium, with potential applications in biotechnology and agricultural sciences.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16599"},"PeriodicalIF":2.2,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13251367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148222407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recommendations of the Polish Zebrafish Society on the use of the zebrafish (<i>Danio rerio</i>) model in biomedical research.","authors":"Piotr Podlasz, Marta Migocka-Patrzałek, Tomasz K Prajsnar, Anna Sarosiak, Przemko Tylzanowski","doi":"10.3389/abp.2026.16545","DOIUrl":"10.3389/abp.2026.16545","url":null,"abstract":"<p><p>The zebrafish (<i>Danio rerio</i>) has become a widely adopted vertebrate model in biomedical research, offering high translational value while supporting the principles of Replacement, Reduction, and Refinement (3Rs). Here, the Polish Zebrafish Society presents comprehensive, field-specific recommendations for the responsible and effective use of zebrafish across major areas of biomedical research. The document summarizes the scientific rationale and experimental advantages of zebrafish-based approaches in oncology, toxicology, neurology and neuropsychiatry, metabolic diseases, immunology, cardiology, and genetic disease modelling. The model's key strengths include rapid development, optical transparency, genetic tractability, and strong conservation of molecular and physiological pathways relevant to human disease. These features enable real-time <i>in vivo</i> analysis of pathological processes, high-throughput pharmacological and toxicological screening, and functional validation of disease-associated genes. By integrating ethical considerations with robust experimental evidence, these recommendations aim to promote standardized implementation of zebrafish models in academic research, pharmaceutical development, and regulatory science. Broad adoption of zebrafish-based approaches can accelerate preclinical discovery, enhance translational relevance, and substantially reduce reliance on higher vertebrate models.</p>","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"73 ","pages":"16545"},"PeriodicalIF":2.2,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13248040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148215831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}