Acta biochimica Polonica最新文献_第3页

The role of TGF-β in the electrotactic reaction of mouse 3T3 fibroblasts in vitro. TGF-β 在小鼠 3T3 成纤维细胞体外电接触反应中的作用

IF 1.4 4区生物学

Acta biochimica Polonica Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.3389/abp.2024.12993

Patrycja Ciesielska, Slawomir Lasota, Sylwia Bobis-Wozowicz, Zbigniew Madeja

{"title":"The role of TGF-β in the electrotactic reaction of mouse 3T3 fibroblasts in vitro.","authors":"Patrycja Ciesielska, Slawomir Lasota, Sylwia Bobis-Wozowicz, Zbigniew Madeja","doi":"10.3389/abp.2024.12993","DOIUrl":"10.3389/abp.2024.12993","url":null,"abstract":"Endogenous electric fields (EFs) serve as a crucial signal to guide cell movement in processes such as wound healing, embryonic development, and cancer metastasis. However, the mechanism underlying cell electrotaxis remains poorly understood. A plausible hypothesis suggests that electrophoretic or electroosmotic forces may rearrange charged components of the cell membrane, including receptors for chemoattractants which induce asymmetric signaling and directional motility. This study aimed to explore the role of Transforming Growth Factor Beta (TGFβ) signaling in the electrotactic reaction of 3T3 fibroblasts. Our findings indicate that inhibiting canonical and several non-canonical signaling pathways originating from the activated TGF-β receptor does not hinder the directed migration of 3T3 cells to the cathode. Furthermore, suppression of TGF-β receptor expression does not eliminate the directional migration effect of 3T3 cells in the electric field. Additionally, there is no observed redistribution of the TGF-β receptor in the electric field. However, our studies affirm the significant involvement of Phosphoinositide 3-Kinase (PI3K) in electrotaxis, suggesting that in our model, its activation is likely associated with factors independent of TGFβ action.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"71 ","pages":"12993"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding mitochondrial potassium channels: 33 years after discovery. 了解线粒体钾通道：发现 33 年后

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2024-05-28 eCollection Date: 2024-01-01 DOI: 10.3389/abp.2024.13126

Adam Szewczyk

{"title":"Understanding mitochondrial potassium channels: 33 years after discovery.","authors":"Adam Szewczyk","doi":"10.3389/abp.2024.13126","DOIUrl":"10.3389/abp.2024.13126","url":null,"abstract":"Mitochondrial investigations have extended beyond their traditional functions, covering areas such as ATP synthesis and metabolism. Mitochondria are now implicated in new functional areas such as cytoprotection, cellular senescence, tumor function and inflammation. The basis of these new areas still relies on fundamental biochemical/biophysical mitochondrial functions such as synthesis of reactive oxygen species, mitochondrial membrane potential, and the integrity of the inner mitochondrial membrane i.e., the passage of various molecules through the mitochondrial membranes. In this view transport of potassium cations, known as the potassium cycle, plays an important role. It is believed that K+ influx is mediated by various potassium channels present in the inner mitochondrial membrane. In this article, we present an overview of the key findings and characteristics of mitochondrial potassium channels derived from research of many groups conducted over the past 33 years. We propose a list of six fundamental observations and most important ideas dealing with mitochondrial potassium channels. We also discuss the contemporary challenges and future prospects associated with research on mitochondrial potassium channels.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"71 ","pages":"13126"},"PeriodicalIF":1.7,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine natural products: potential agents for depression treatment. 海洋天然产品：治疗抑郁症的潜在药物。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2024-04-30 eCollection Date: 2024-01-01 DOI: 10.3389/abp.2024.12569

Xunqiang Wang, Cece Yang, Xing Zhang, Caiping Ye, Wenping Liu, Chengmin Wang

引用次数: 0

Sensing antibody functions with a novel CCR8-responsive engineered cell. 利用新型 CCR8 响应工程细胞感知抗体功能。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2024-04-29 eCollection Date: 2024-01-01 DOI: 10.3389/abp.2024.12185

Jianyu Hao, Yitong Lv, Xufeng Xiao, Lidan Li, Changyuan Yu

{"title":"Sensing antibody functions with a novel CCR8-responsive engineered cell.","authors":"Jianyu Hao, Yitong Lv, Xufeng Xiao, Lidan Li, Changyuan Yu","doi":"10.3389/abp.2024.12185","DOIUrl":"10.3389/abp.2024.12185","url":null,"abstract":"Human chemokine receptor 8 (CCR8) is a promising drug target for immunotherapy of cancer and autoimmune diseases. Monoclonal antibody-based CCR8 targeted treatment shows significant inhibition in tumor growth. The inhibition of CCR8 results in the improvement of antitumor immunity and patient survival rates by regulating tumor-resident regulatory T cells. Recently monoclonal antibody drug development targeting CCR8 has become a research hotspot, which also promotes the advancement of antibody evaluation methods. Therefore, we constructed a novel engineered customized cell line HEK293-cAMP-biosensor-CCR8 combined with CCR8 and a cAMP-biosensor reporter. It can be used for the detection of anti-CCR8 antibody functions like specificity and biological activity, in addition to the detection of antibody-dependent cell-mediated cytotoxicity and antibody-dependent-cellular-phagocytosis. We obtained a new CCR8 mAb 22H9 and successfully verified its biological activities with HEK293-cAMP-biosensor-CCR8. Our reporter cell line has high sensitivity and specificity, and also offers a rapid kinetic detection platform for evaluating anti-CCR8 antibody functions.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"71 ","pages":"12185"},"PeriodicalIF":1.7,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin promotes the normalization of abnormal blood vessels after radiofrequency ablation deficiency in hepatocellular carcinoma by microRNA-302b-3p targeting thioredoxin-interacting protein. 二甲双胍通过microRNA-302b-3p靶向硫氧还蛋白相互作用蛋白促进肝细胞癌射频消融缺陷后异常血管的正常化。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-12-22 DOI: 10.18388/abp.2023_6296

Haigang Niu, Shuying Dong, GuoMing Li, Shilun Wu, WenBing Sun

{"title":"Metformin promotes the normalization of abnormal blood vessels after radiofrequency ablation deficiency in hepatocellular carcinoma by microRNA-302b-3p targeting thioredoxin-interacting protein.","authors":"Haigang Niu, Shuying Dong, GuoMing Li, Shilun Wu, WenBing Sun","doi":"10.18388/abp.2023_6296","DOIUrl":"https://doi.org/10.18388/abp.2023_6296","url":null,"abstract":"Metformin has shown great promise in the treatment of HCC. Radiofrequency ablation (RFA) deficiency results in recurrence and metastasis of remaining HCC tumors. Here, we aimed to investigate the role and mechanism of metformin in HCC after RFA deficiency. HCC cell line Hep-G2 was selected to simulate RFA deficiency and named HepG2-H cells. After treating cells with different concentrations of metformin (2.5, 5, 10 μM) or transfecting related plasmids, cell proliferation, migration, invasion, apoptosis and angiogenesis were detected, in vitro permeability test was performed, and an angiogenesis-related protein VEGFA was analyzed. The residual HCC model after RFA deficiency was established in mice. Metformin was administered by gavage to detect changes in tumor volume and weight, and CD31 staining was used to observe microvessels. The targeting relationship between miR-302b-3p and TXNIP was demonstrated by the bioinformatics website, dual-luciferase reporter assay, and RNA pull-down assay. The results found that metformin inhibited RFA deficiency-induced growth and angiogenesis of HCC cells in vitro. miR-302b-3p counteracted the therapeutic effect of metformin on RFA deficiency. miR-302b-3p targeted regulation of TXNIP. The up-regulation of TXNIP reversed the effects of overexpression of miR-302b-3p on RFA-deficient HCC cells. Metformin inhibited RFA-deficiency-induced HCC growth and tumor vascular abnormalities in vivo. Overall, metformin promotes the normalization of abnormal blood vessels after RFA deficiency in HCC by miR-302b-3p targeting TXNIP, which can be used to prevent the progression of HCC after RFA.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"83 13","pages":"1005-1014"},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138945333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin promotes the normalization of abnormal blood vessels after radiofrequency ablation deficiency in hepatocellular carcinoma by microRNA-302b-3p targeting thioredoxin-interacting protein. 二甲双胍通过microRNA-302b-3p靶向硫氧还蛋白相互作用蛋白促进肝细胞癌射频消融缺陷后异常血管的正常化。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-12-22 DOI: 10.18388/abp.2018_6296

HaiGang Niu, ShuYing Dong, GuoMing Li, ShiLun Wu, WenBing Sun

{"title":"Metformin promotes the normalization of abnormal blood vessels after radiofrequency ablation deficiency in hepatocellular carcinoma by microRNA-302b-3p targeting thioredoxin-interacting protein.","authors":"HaiGang Niu, ShuYing Dong, GuoMing Li, ShiLun Wu, WenBing Sun","doi":"10.18388/abp.2018_6296","DOIUrl":"10.18388/abp.2018_6296","url":null,"abstract":"Metformin has shown great promise in the treatment of HCC. Radiofrequency ablation (RFA) deficiency results in recurrence and metastasis of remaining HCC tumors. Here, we aimed to investigate the role and mechanism of metformin in HCC after RFA deficiency. HCC cell line Hep-G2 was selected to simulate RFA deficiency and named HepG2-H cells. After treating cells with different concentrations of metformin (2.5, 5, 10 μM) or transfecting related plasmids, cell proliferation, migration, invasion, apoptosis and angiogenesis were detected, in vitro permeability test was performed, and an angiogenesis-related protein VEGFA was analyzed. The residual HCC model after RFA deficiency was established in mice. Metformin was administered by gavage to detect changes in tumor volume and weight, and CD31 staining was used to observe microvessels. The targeting relationship between miR-302b-3p and TXNIP was demonstrated by the bioinformatics website, dual-luciferase reporter assay, and RNA pull-down assay. The results found that metformin inhibited RFA deficiency-induced growth and angiogenesis of HCC cells in vitro. miR-302b-3p counteracted the therapeutic effect of metformin on RFA deficiency. miR-302b-3p targeted regulation of TXNIP. The up-regulation of TXNIP reversed the effects of overexpression of miR-302b-3p on RFA-deficient HCC cells. Metformin inhibited RFA-deficiency-induced HCC growth and tumor vascular abnormalities in vivo. Overall, metformin promotes the normalization of abnormal blood vessels after RFA deficiency in HCC by miR-302b-3p targeting TXNIP, which can be used to prevent the progression of HCC after RFA.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 4","pages":"1005-1014"},"PeriodicalIF":1.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138827632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic changes of serum miR-105-3p expression and prognostic value evaluation of postoperative thyroid cancer. 甲状腺癌术后血清 miR-105-3p 表达的动态变化及预后价值评估

IF 1.4 4区生物学

Acta biochimica Polonica Pub Date : 2023-12-19 DOI: 10.18388/abp.2018_6398

JianPing Zhou, XiaoLong Song, YuFang Li, Yu Song, Long Wei, Ru Yang

{"title":"Dynamic changes of serum miR-105-3p expression and prognostic value evaluation of postoperative thyroid cancer.","authors":"JianPing Zhou, XiaoLong Song, YuFang Li, Yu Song, Long Wei, Ru Yang","doi":"10.18388/abp.2018_6398","DOIUrl":"10.18388/abp.2018_6398","url":null,"abstract":"Objective: To explore the dynamic changes of serum miR-105-3p expression after thyroid cancer surgery and its correlation with clinicopathological manifestations and to evaluate its clinical value as a potential biomarker after surgery.Methods: A total of 100 thyroid cancer patients admitted to Shaanxi Provincial People's Hospital from November 2020 to August 2021 were selected as the research objects. The aim was to detect the expression of serum miR-105-3p in patients and its correlation with tumor pathological characteristics (pathological type, tumor differentiation, TNM stage, lymph node metastasis), and to detect the dynamic changes of postoperative serum miR-105-3p in patients to evaluate its prognostic value as a potential biomarker.Results: The level of serum miR-105-3p increases in patients with well-differentiated thyroid cancer and lymph node metastasis; the level of serum miR-105-3p gradually decreases with the passage of time after surgery, and there is a significant difference between 4 d after surgery and before surgery; serum miR-105-3p level can significantly distinguish between patients with poor prognosis and good prognosis within 2 years after the operation, and it can predict the improvement of the prognosis of thyroid cancer after surgery.Conclusions: The level of serum miR-105-3p is closely related to the degree of differentiation and lymph node metastasis in patients with thyroid cancer. Its level gradually decreases with the passage of time after surgery. It has a good diagnostic value for the prognosis of thyroid cancer after surgery and when it is expected to become a thyroid cancer surgery. Potential biomarkers for post-diagnosis.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 4","pages":"997-1003"},"PeriodicalIF":1.4,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic changes of serum miR-105-3p expression and prognostic value evaluation of postoperative thyroid cancer. 甲状腺癌术后血清 miR-105-3p 表达的动态变化及预后价值评估

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-12-19 DOI: 10.18388/abp.2023_6398

JianPing Zhou, Xiaolong Song, Yufang Li, Yu Song, Long Wei, Ru Yang

{"title":"Dynamic changes of serum miR-105-3p expression and prognostic value evaluation of postoperative thyroid cancer.","authors":"JianPing Zhou, Xiaolong Song, Yufang Li, Yu Song, Long Wei, Ru Yang","doi":"10.18388/abp.2023_6398","DOIUrl":"https://doi.org/10.18388/abp.2023_6398","url":null,"abstract":"OBJECTIVE\u0000To explore the dynamic changes of serum miR-105-3p expression after thyroid cancer surgery and its correlation with clinicopathological manifestations and to evaluate its clinical value as a potential biomarker after surgery.\u0000\u0000\u0000METHODS\u0000A total of 100 thyroid cancer patients admitted to Shaanxi Provincial People's Hospital from November 2020 to August 2021 were selected as the research objects. The aim was to detect the expression of serum miR-105-3p in patients and its correlation with tumor pathological characteristics (pathological type, tumor differentiation, TNM stage, lymph node metastasis), and to detect the dynamic changes of postoperative serum miR-105-3p in patients to evaluate its prognostic value as a potential biomarker.\u0000\u0000\u0000RESULTS\u0000The level of serum miR-105-3p increases in patients with well-differentiated thyroid cancer and lymph node metastasis; the level of serum miR-105-3p gradually decreases with the passage of time after surgery, and there is a significant difference between 4 d after surgery and before surgery; serum miR-105-3p level can significantly distinguish between patients with poor prognosis and good prognosis within 2 years after the operation, and it can predict the improvement of the prognosis of thyroid cancer after surgery.\u0000\u0000\u0000CONCLUSIONS\u0000The level of serum miR-105-3p is closely related to the degree of differentiation and lymph node metastasis in patients with thyroid cancer. Its level gradually decreases with the passage of time after surgery. It has a good diagnostic value for the prognosis of thyroid cancer after surgery and when it is expected to become a thyroid cancer surgery. Potential biomarkers for post-diagnosis.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" 12","pages":"997-1003"},"PeriodicalIF":1.7,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138960686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JNK promotes the progression of castration-resistant prostate cancer. JNK 促进了耐受性前列腺癌的进展。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-12-15 DOI: 10.18388/abp.2020_6610

Yigeng Feng, Hongwen Cao, Dan Wang, Lei Chen, Renjie Gao, Peng Sun

{"title":"JNK promotes the progression of castration-resistant prostate cancer.","authors":"Yigeng Feng, Hongwen Cao, Dan Wang, Lei Chen, Renjie Gao, Peng Sun","doi":"10.18388/abp.2020_6610","DOIUrl":"10.18388/abp.2020_6610","url":null,"abstract":"Background: Prostate cancer is one of the most common cancers in men worldwide. This study aims to elucidate the roles of c-Jun N-terminal kinase (JNK) in the progression of castration-resistant prostate cancer (CRPC).Methods: JNK overexpressing and knockdown cell lines were established on the PC-3 prostate cell line. qPCR and Western blotting were performed to determine the mRNA and protein levels of target genes in prostate tissues and cell lines. MTT and Matrigel invasion assays were conducted to evaluate the cell viability and invasive ability, respectively. The Kaplan-Meier estimator was performed to estimate the overall survival rate and second progression-free survival rate. Pearson's correlation coefficient was used to evaluate the relationship between JNK and prostate-specific antigen (PSA).Results: Relative JNK expression was correlated with Gleason score and PSA value in patients with CRPC. Kaplan-Meier analysis revealed that patients with low JNK expression exhibited high overall survival and second progression-free survival rate. In vitro assays demonstrated that JNK overexpression promoted cell viability and invasion as well as the protein expressions of extracellular signal-regulated kinase (ERK) and matrix metalloproteinase 1 (MMP1) in PC-3 cell lines.Conclusions: JNK overexpression promotes the development of CRPC via the regulation of ERK and MMP1.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 4","pages":"817-822"},"PeriodicalIF":1.7,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping the substrate-binding subsite specificity of a Porphyromonas gingivalis Tpr peptidase 绘制牙龈卟啉单胞菌 Tpr 肽酶底物结合位点特异性图谱

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-12-08 DOI: 10.18388/abp.2020_6904

Dominika Staniec, Wioletta Rut, Marcin Drag, Michał Burmistrz, Michael Kitching, Jan Potempa

引用次数: 0