Acta biochimica Polonica最新文献_第4页

Genetic association between vitamin D receptor gene and Saudi patients confirmed with Familial Hypercholesterolemia. 维生素D受体基因与沙特确诊家族性高胆固醇血症患者的遗传关系

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-28 DOI: 10.18388/abp.2020_6638

May Salem Al-Nbaheen

{"title":"Genetic association between vitamin D receptor gene and Saudi patients confirmed with Familial Hypercholesterolemia.","authors":"May Salem Al-Nbaheen","doi":"10.18388/abp.2020_6638","DOIUrl":"10.18388/abp.2020_6638","url":null,"abstract":"Introduction: Familial Hypercholesterolemia (FH) is a common condition caused by inherited genetic abnormalities. Inadequate clearance of the circulating low-density lipoproteins (LDL) is the primary cause of the excessive concentrations of LDL seen in FH patients. The relation with vitamin D deficiency and vitamin D receptor (VDR) gene is well documented in the Saudi Arabia.Aim: The aim of this study was to investigate the role of molecular analysis studied between FH patients and fours polymorphisms associated with VDR gene in Saudi Population.Methods: In this case-control study, 120 patients were selected, and 50 patients were confirmed as FH and 70 subjects were confirmed as healthy controls. Genotyping was performed with polymerase chain reaction followed by restriction fragment length polymorphism analysis using ApaI, BsmI, TaqI and FokI polymorphisms in the VDR gene.Results: The current study results confirmed no association between clinical characteristics studied between FH cases and controls (p>0.05). Hardy Weinberg Equilibrium analysis was present in ApaI and FokI polymorphisms (p<0.05). Only ApaI (C vs A: OR-15.1 (95% CI:5.78-39.41); p<0.001; AC+CC vs AA: OR-6.59 (95% CI:2.42-17.95); p=0.0006) and BsmI (G vs A: OR-2.88 (95% CI:1.54-5.38); p=0.0006 and AG+GG vs AA: OR-3.79 (95% CI:1.72-8.35); p=0.0007) polymorphisms showed both allele and genotype association between FH patients and controls. ANOVA analysis confirmed that TG levels were associated (p=0.02) with combination of heterozygous and homozygous genotypes present in all four polymorphisms studied in this population.Conclusion: ApaI and BsmI polymorphisms in the VDR gene showed association with FH patients in the Saudi Population.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"829-834"},"PeriodicalIF":1.7,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNA-301 As a molecule promoting necrotizing enterocolitis by inducing inflammation. miRNA-301是通过诱导炎症促进坏死性小肠结肠炎的分子。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-28 DOI: 10.18388/abp.2020_6806

Dajun Zou, Fude Hu, Qili Zhou, Xiaoqing Xu

{"title":"miRNA-301 As a molecule promoting necrotizing enterocolitis by inducing inflammation.","authors":"Dajun Zou, Fude Hu, Qili Zhou, Xiaoqing Xu","doi":"10.18388/abp.2020_6806","DOIUrl":"10.18388/abp.2020_6806","url":null,"abstract":"Objective: Necrotizing enterocolitis (NEC) is a devastating inflammatory disease with high morbidity and mortality, mainly affecting premature infants. This study aimed to explore the role of miRNA-301a in the pathogenesis of NEC.Methods: The differentially expressed miRNAs and mRNAs were screened by collating RNA-Seq data from the GEO database of intestinal tissue samples. The differential miRNA-mRNAs regulatory network was constructed based on functional enrichment analysis. Newborn BALB/c mice were used to establish the NEC model. Haematoxylin and eosin staining was used to assess intestinal damage. The levels of IL-8 and TNF-α in mouse serum were evaluated by ELISA. qRT-PCR was used to detect the expression of miRNA-301a in intestinal tissues.Results: Bioinformatics analysis showed that miRNA-301a was involved in intestinal lesions. Intestinal tissue damage was reduced and serum levels of the inflammatory cytokines IL-8 and TNF-α were lower in NEC model mice treated with miRNA-301a antagonists. The level of miRNA-301a in intestinal tissues of NEC model mice was significantly higher than in the control group and miRNA-301a antagonists treated group.Conclusion: miRNA-301a plays an important role in the pathogenesis of NEC by promoting inflammation, and is a potential therapeutic target of NEC.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"905-910"},"PeriodicalIF":1.7,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decrease of prothrombin level during thrombolysis in acute myocardium infarction. 急性心肌梗死溶栓过程中凝血酶原水平的降低。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-27 DOI: 10.18388/abp.2020_6962

Daria S Korolova, Alexander M Parkhomenko, Volodymyr Chernyshenko, Tamara M Chernyshenko, Nadiya M Druzhyna, Olha V Hornytska, Tetyana M Platonova

{"title":"Decrease of prothrombin level during thrombolysis in acute myocardium infarction.","authors":"Daria S Korolova, Alexander M Parkhomenko, Volodymyr Chernyshenko, Tamara M Chernyshenko, Nadiya M Druzhyna, Olha V Hornytska, Tetyana M Platonova","doi":"10.18388/abp.2020_6962","DOIUrl":"10.18388/abp.2020_6962","url":null,"abstract":"Previously, the direct interactions of Bβ26-42 fibrin residues with prothrombin were demonstrated. It was also shown that forming prothrombin complexes with E- or DDE-fragments causes non-enzymatic prothrombin activation. The direct measuring of the prothrombin level in the blood plasma of patients with acute myocardial infarction (AMI) allowed us to find a situation where such an activation can occur in vivo. Blood coagulation parameters in the blood plasma of patients with AMI were measured at 2 hours, three days, and seven days after the thrombolysis by streptokinase accompanied with intravenous administration of anticoagulants: unfractionated high molecular weight heparin (HMWH) and low-molecular-weight heparin (LMWH). The prothrombin level in the blood plasma of patients with AMI was normal before thrombolytic therapy and substantially decreased after streptokinase administration. This effect was prominent in the case of concomitant anticoagulant therapy with LMWH and was not observed when HMWH was applied. It can be explained by the fact that LMWH preferentially inhibits factor Xa, while the HMWH is an effective inhibitor of both factor Xa and thrombin. This observation suggested that the prothrombin level decrease was caused by the thrombin-like activity and possible autolysis of prothrombin by thrombin. Also, thrombolytic therapy with streptokinase caused the accumulation of fibrin degradation products (FDPs), some of which were able to bind prothrombin. The dramatic decrease of prothrombin level in the blood plasma of patients with AMI during thrombolysis allowed us to conclude the non-enzymatic prothrombin activation with the following autolysis of prothrombin that contributes to the pathology.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"991-995"},"PeriodicalIF":1.7,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-221-3p promotes post-burn HUVEC proliferation, migration, and angiogenesis by regulating CDKN1B. MicroRNA-221-3p通过调节CDKN1B促进烧伤后HUVEC的增殖、迁移和血管生成。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-22 DOI: 10.18388/abp.2020_6295

Kun Miao, Fei Xie, JinGui Lin

{"title":"MicroRNA-221-3p promotes post-burn HUVEC proliferation, migration, and angiogenesis by regulating CDKN1B.","authors":"Kun Miao, Fei Xie, JinGui Lin","doi":"10.18388/abp.2020_6295","DOIUrl":"10.18388/abp.2020_6295","url":null,"abstract":"Background and objective: Previous studies have shown that miR-221-3p plays an important role in vascular remodeling, but it is unclear whether it contributes to angiogenesis after burn injury. The purpose of this study was to investigate the effect of miR-221-3p on angiogenesis in HUVECs after burn injury and to reveal its underlying molecular mechanism.Methods: The burn HUVECs model was established by heat treatment. Plasmid or oligonucleotide transfection altered the expression of miR-221-3p and CDKN1B in HUVECs. MTT, colony formation, Transwell, flow cytometry, and tube formation experiments were applied to assess the proliferation, migration, apoptosis, cell cycle, and tube formation capacity of HUVECs. miR-221-3p, CDKN1B, Ki-67, and PCNA expression was assessed by RT-qPCR or Western blot. The dual-luciferase reporter assay verified the targeting relationship between miR-221-3p and CDKN1B.Results: miR-221-3p was lowly expressed and CDKN1B was highly expressed in burn HUVECs. Overexpression of miR-221-3p promoted the proliferation, migration, and tube formation ability of burn HUVECs and inhibited apoptosis and the proportion of cells in the G0/G1 phase, whereas overexpression of CDKN1B had the opposite effect. Knockdown of miR-221-3p further inhibited the angiogenic capacity of burn HUVECs, but this effect was reversed by knockdown of CDKN1B. Mechanistically, miR-221-3p targeted CDKN1B.Conclusion: miR-221-3p improves the angiogenesis of burn HUVECs by targeting CDKN1B expression, and the miR-221-3p/CDKN1B axis may serve as a potential molecular target for future burn therapy.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"785-790"},"PeriodicalIF":1.7,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138290031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and biochemical mechanisms of diabetic encephalopathy. 糖尿病性脑病的分子生化机制。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-22 DOI: 10.18388/abp.2020_6953

Igor Belenichev, Olena Aliyeva, Olena Popazova, Nina Bukhtiyarova

引用次数: 0

Imiquimod-induced psoriasis model: induction protocols, model characterization and factors adversely affecting the model. 吡喹莫德诱导的银屑病模型:诱导方案、模型特性和影响模型的不利因素。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-22 DOI: 10.18388/abp.2020_6426

Manahel Mahmood Alsabbagh

引用次数: 0

MicroRNA-508-3p regulates the proliferation of human lung cancer cells by targeting G1 to S phase transition 1 (GSPT1) protein. MicroRNA-508-3p 通过靶向 G1 到 S 期转变 1 (GSPT1) 蛋白调节人类肺癌细胞的增殖。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-22 DOI: 10.18388/abp.2020_6660

Xingyou Chen, Chen Feng, Jiliang Zha, Zihao Shen, Wei Ji

{"title":"MicroRNA-508-3p regulates the proliferation of human lung cancer cells by targeting G1 to S phase transition 1 (GSPT1) protein.","authors":"Xingyou Chen, Chen Feng, Jiliang Zha, Zihao Shen, Wei Ji","doi":"10.18388/abp.2020_6660","DOIUrl":"10.18388/abp.2020_6660","url":null,"abstract":"Purpose: Due to its crucial cancer regulatory role, microRNA-508-3p has been reported as a potential therapeutic anticancer molecular target. The present work encompassed the molecular characterization of microRNA-508-3p in lung cancer emphasizing on understanding the possible mechanism of its regulatory action.Methods: qRT-PCR was performed to estimate the relative gene expression of microRNA-508-p in the tissue samples. The proliferation of cancer cells was determined by cell counting kit-8. The colony formation from cancer cells was analyzed by clonogenic assay. Mitotic phase distribution was understood by employing the flow cytometric technique. Edu-Hoechst staining was used for the assessment of cell viability. In silico analysis and dual-luciferase assay were used for target identification of microRNA-508-3p in lung cancer. Immunofluorescence and western blotting studies were carried out for relative protein expression. The rat models were used for performing the in vivo experimental procedures.Results: The study showed the significant down-regulation of microRNA-508-3p in lung cancer. The lower expression levels of microRNA-508-3p were shown to be associated with poor survival of lung cancer patients. The over-expression of microRNA-508-3p was found to decline the proliferation and viability of cancer cells together with the induction of mitotic cell cycle arrest at G1 by targeting G1 to S phase transition 1 (GSPT1) protein. MicroRNA-508-3p up-regulation inhibited the in vivo tumor growth in rat models.Conclusion: Our study identifies miR-508-3p as a pivotal regulator of lung cancer cell proliferation by targeting the GSPT1 protein. This highlights its potential as a tumor suppressor and a therapeutic target for lung cancer. Our findings offer mechanistic insights into miRNA-mediated cancer progression, prompting further research in this intricate regulatory network.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":"70 4","pages":"835-841"},"PeriodicalIF":1.7,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of microbiological safety of dry cat foods marketed in Poland. 波兰市售干猫食品微生物安全性调查。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-15 DOI: 10.18388/abp.2020_6921

Joanna Ziętara-Wysocka, Olga Sierawska, Cansel Taskin, Agata Poniewierska-Baran, Dominika Bębnowska, Rafał Hrynkiewicz, Filip Lewandowski, Paulina Niedźwiedzka-Rystwej

{"title":"Investigation of microbiological safety of dry cat foods marketed in Poland.","authors":"Joanna Ziętara-Wysocka, Olga Sierawska, Cansel Taskin, Agata Poniewierska-Baran, Dominika Bębnowska, Rafał Hrynkiewicz, Filip Lewandowski, Paulina Niedźwiedzka-Rystwej","doi":"10.18388/abp.2020_6921","DOIUrl":"10.18388/abp.2020_6921","url":null,"abstract":"Pets are inhabiting more and more human homes every year. In 2020, the cat population in Europe was 110 million, including 6.8 million in Poland. Dry food is the most popular dietary model for cats because of its easy storage and efficient satisfaction of pet needs. The high processing temperature of dry food reduces the chance of microbial contamination, but this can occur later, during post-production or storage in the pet's caregiver's home or, in the case of weighed foods, in the store. The purpose of this study was to investigate the microbiological safety of dry feed sold in the original manufacturer's packaging and the same feed from the same manufacturers sold in a retail store by weight. Six discriminants, presence of Salmonella spp., number of coliforms, number of coagulase-positive staphylococci, determination of yeast and mould counts, Enterobacteriaceae count, Listeria monocytogenes and determination of total aerobic microbial count were used for the analysis. Then, cat food was then stored for 45 days according to the manufacturer's recommendations. Based on the samples tested both after opening and after storage, it was concluded that the dry cat food analyzed posed a law microbiological risk to animals and humans.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"971-977"},"PeriodicalIF":1.7,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relative expression levels of growth hormone gene and growth rate in Indian major carp species. 印度主要鲤鱼种生长激素基因的相对表达量与生长速率。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-14 DOI: 10.18388/abp.2020_6864

Shahid Sherzada, Muhammad Nauman Sharif, Qurban Ali, Saeed Akram Khan, Tawaf Ali Shah, Mohamed A M El-Tabakh, Tariq Aziz, Ghulam Nabi, Metab Alharbi, Thamer H Albekairi, Abdullah F Alasmari

{"title":"Relative expression levels of growth hormone gene and growth rate in Indian major carp species.","authors":"Shahid Sherzada, Muhammad Nauman Sharif, Qurban Ali, Saeed Akram Khan, Tawaf Ali Shah, Mohamed A M El-Tabakh, Tariq Aziz, Ghulam Nabi, Metab Alharbi, Thamer H Albekairi, Abdullah F Alasmari","doi":"10.18388/abp.2020_6864","DOIUrl":"10.18388/abp.2020_6864","url":null,"abstract":"The phenomenon of growth is a leading factor for aquaculture success. The uneven growth of major Indian carps (Labeo rohita, Catla catla, and Cirrhinus mrigala) is a serious issue in fish culture from an economic point of view. The growth hormone (GH) gene is crucial for selection in commercially cultivated fish species for better growth and production. Indian major carp (L. rohita, C. catla, and C. mrigala) are commonly cultured in Pakistan. The GH expression was examined using qPCR to understand growth in fish species better. Muscle tissue samples (n=480) from 160 individuals of the same age were collected from three species (L. rohita, C. catla, and C. mrigala). Individuals were divided into two groups (high-weight and low-weight groups), cultured under normal conditions. The housekeeping gene β-actin validated GH expression in fast and slow-growing fishes from the same species. Results showed that GH expression varies across species and fish specimens that overweight their counterpart feature have higher GH expression. A selection for overweight fish in the aquaculture breeding systems is preferable as those fish could inherit their genomics to the future cohort, enhancing production, and commercial profit for farmers. Comprehensive research about different growth genes and the environmental aspects that influence fish growth is mandatory. No work has been reported regarding the growth gene analysis of fish from Pakistan. This report was Pakistan's first and baseline study regarding growth analysis of main culturable fish species at the molecular level.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"943-949"},"PeriodicalIF":1.7,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107589943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, preparation and investigation of leaf extracts of Aloe barbadensis for its remedial effects on tumor necrosis factor alpha (TNF-α) and interleukin (IL-6) by in vivo and in silico approaches in experimental rats. 芦荟叶提取物的分离、制备及其对实验大鼠肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的治疗作用。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-08 DOI: 10.18388/abp.2020_6827

Iram Khurshaid, Sobia Ilyas, Nureen Zahra, Suhail Ahmad, Tariq Aziz, Fahad Al-Asmari, Sanaa Almowallad, Rehab F Al-Massabi, Yasmene F Alanazi, Aminah A Barqawi, Roaa Mohammed Tahir Kassim, Abdulhakeem S Alamri, Majid Alhomrani, Manal Y Sameeh

{"title":"Isolation, preparation and investigation of leaf extracts of Aloe barbadensis for its remedial effects on tumor necrosis factor alpha (TNF-α) and interleukin (IL-6) by in vivo and in silico approaches in experimental rats.","authors":"Iram Khurshaid, Sobia Ilyas, Nureen Zahra, Suhail Ahmad, Tariq Aziz, Fahad Al-Asmari, Sanaa Almowallad, Rehab F Al-Massabi, Yasmene F Alanazi, Aminah A Barqawi, Roaa Mohammed Tahir Kassim, Abdulhakeem S Alamri, Majid Alhomrani, Manal Y Sameeh","doi":"10.18388/abp.2020_6827","DOIUrl":"10.18388/abp.2020_6827","url":null,"abstract":"Aloe barbadensis is a stemless plant with a length of 60-100 cm with juicy leaves which is used for its remedial and healing properties in different suburbs of various countries. The present study was conducted to investigate the effect of A. barbadensis leaf extract (aqueous and ethanolic) in yeast induced pyrexia and acetic acid induced writhing in rat model to evaluate the antipyretic biomarkers and its phytochemical screening with computational analysis. For analgesic activity model 60 Albino rats (160-200 kg) were divided into four groups. Of the 4 groups, control consisted of 6 rats (Group I) treated with normal saline, standard comprised of 6 rats treated with drug diclofenac (Group I). Experimental groups consisted of 48 rats, treated with A. barbadensis ethanolic and aqueous leaf extracts at doses of 50 mg/kg, 100 mg/kg, 200 mg/kg, and 400 mg/kg (Group III. IV). For antipyretic activity group division was same as in analgesic activity. All groups were treated the same as in the analgesic activity except for the second group which was treated with paracetamol. In both antipyretic and analgesic activity at the dose of 400 mg/kg, group III showed significant inhibition. TNF-α and IL-6 showed significant antipyretic activity at a dose of 400 mg/kg. For molecular docking aloe emodin and cholestanol were used as ligand molecules to target proteins Tnf-α and IL-6. Acute oral toxicity study was performed. There was no mortality even at the dose of 2000 mg/kg. Quantitative and qualitative phytochemical screening was performed for the detection of various phytochemicals. Hence, A. barbadensis leaf extracts can be used in the form of medicine for the treatment of pain and fever.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"927-933"},"PeriodicalIF":1.7,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71520159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0