Identification of AHNAK expression associated with the pathogenesis of chronic obstructive pulmonary disease by bioinformatic analysis.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Acta biochimica Polonica Pub Date : 2023-12-05 DOI:10.18388/abp.2020_6041

Chunhui Zhang, Yu Liu

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引用次数: 0

Abstract

Background: Chronic obstructive pulmonary disease (COPD) was a risk factor for lung cancer tumorigenesis. This study aimed to discover novel diagnostic biomarkers for COPD patients and determine their underlying pathogenetic mechanisms.

Materials and methods: Differentially expressed genes (DEGs) in COPD samples and normal controls were analyzed and utilized to construct a network associated with a high risk for COPD occurrence. Enrichment analysis was applied on the strength of Gene Ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The RT-qPCR analysis was performed to determine 10 hub genes in COPD. ELISA assay was utilized to measure IL-1β, IL-6, and IL-10 levels. Spearman's correlation analysis was conducted to detect the correlation between inflammatory cytokines and AHNAK expression. Cell proliferation and apoptosis were evaluated by CCK-8 and flow cytometry assays.

Results: AHNAK was significantly increased in COPD serum samples compared with non-COPD smokers and strongly correlated with inflammation. AHNAK level could also discriminate COPD from non-COPD with high accuracy.

Conclusion: AHNAK may be a feasible biomarker playing crucial functions in the diagnosis and progression of COPD.

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通过生物信息学分析确定与慢性阻塞性肺病发病机制相关的 AHNAK 表达。

背景：慢性阻塞性肺疾病（COPD）是肺癌发生的一个危险因素。本研究旨在发现慢性阻塞性肺病患者的新型诊断生物标志物，并确定其潜在的发病机制：对慢性阻塞性肺病样本和正常对照中的差异表达基因（DEGs）进行了分析，并利用这些差异表达基因构建了一个与慢性阻塞性肺病高风险相关的网络。根据基因本体（GO）注释和《京都基因组百科全书》（KEGG）通路分析进行了富集分析。通过 RT-qPCR 分析确定了慢性阻塞性肺病的 10 个关键基因。利用 ELISA 法检测 IL-1β、IL-6 和 IL-10 的水平。斯皮尔曼相关分析检测了炎性细胞因子与 AHNAK 表达之间的相关性。细胞增殖和凋亡通过 CCK-8 和流式细胞术检测进行评估：结果：与非慢性阻塞性肺病吸烟者相比，慢性阻塞性肺病血清样本中的 AHNAK 明显升高，且与炎症密切相关。AHNAK水平还能准确区分慢性阻塞性肺病和非慢性阻塞性肺病：结论：AHNAK可能是一种可行的生物标志物，在慢性阻塞性肺病的诊断和进展中发挥着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta biochimica Polonica 生物-生化与分子生物学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Acta Biochimica Polonica is a journal covering enzymology and metabolism, membranes and bioenergetics, gene structure and expression, protein, nucleic acid and carbohydrate structure and metabolism.