Acta biochimica Polonica最新文献_第5页

Competitive binding of circCCDC6 to microRNA-128-3p activates TXNIP/NLRP3 pathway and promotes cerebral ischemia-reperfusion defects. circCCDC6与microRNA-128-3p的竞争性结合激活TXNIP/NLRP3通路并促进脑缺血再灌注缺陷。

IF 1.4 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-07 DOI: 10.18388/abp.2020_6552

ChongShu Wang, MingMing Dong, XiaoYi Zhang, XiaoYu Wang, Yan Zhao, Yunpeng Cao

{"title":"Competitive binding of circCCDC6 to microRNA-128-3p activates TXNIP/NLRP3 pathway and promotes cerebral ischemia-reperfusion defects.","authors":"ChongShu Wang, MingMing Dong, XiaoYi Zhang, XiaoYu Wang, Yan Zhao, Yunpeng Cao","doi":"10.18388/abp.2020_6552","DOIUrl":"10.18388/abp.2020_6552","url":null,"abstract":"Objective: Circular RNAs (circRNAs) are enriched in the brain and involved in various central nervous system diseases. The potential role of circCCDC6 in cerebral ischemia-reperfusion defects was partly elucidated in the work.Methods: A middle cerebral artery occlusion/reperfusion (MCAO/R) rat model and an oxygen-glucose deprivation and re-oxygenation (OGD/R)-treated SH-SY5Y cell model were constructed. CircCCDC6 expression in the two models was examined, and circCCDC6-involved mechanisms in neuronal pyroptosis and inflammation were analyzed through loss- and gain-of-function assays.Results: MCAO/R rat brain tissues and OGD/R-treated SH-SY5Y cells exhibited upregulated circCCDC6. Silencing circCCDC6 attenuated neuronal pyroptosis and inflammation in the brain tissue of MCAO/R rats. Overexpressing circCCDC6 or inhibiting miR-128-3p stimulated OGD/R-induced pyroptosis and inflammation in SH-SY5Y cells, while upregulating miR-128-3p attenuated OGD/R injury. CircCCDC6 silencing-induced effects on SH-SY5Y cells were antagonized by TXNIP overexpression.Conclusion: Mechanistically, circCCDC6 mediates miR-128-3p and activates TXNIP/NLRP3, thereby promoting OGD/R-induced neuronal pyroptosis and inflammation. CircCCDC6 may provide a new strategy for the treatment of MCAO/R.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"807-815"},"PeriodicalIF":1.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71476845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatic Mcpip1 regulates adaptation to food restriction in mice. 肝Mcpip1调节小鼠对食物限制的适应。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-06 DOI: 10.18388/abp.2020_6819

Olga Mucha, Bozena Skupien-Rabian, Alicja Slota, Katarzyna Trzos, Natalia Pydyn, Bartosz Podlewski, Jolanta Jura, Jerzy Kotlinowski

{"title":"Hepatic Mcpip1 regulates adaptation to food restriction in mice.","authors":"Olga Mucha, Bozena Skupien-Rabian, Alicja Slota, Katarzyna Trzos, Natalia Pydyn, Bartosz Podlewski, Jolanta Jura, Jerzy Kotlinowski","doi":"10.18388/abp.2020_6819","DOIUrl":"10.18388/abp.2020_6819","url":null,"abstract":"Monocyte-chemoattractant protein-induced protein 1 (MCPIP1, or Regnase-1) is an endoribonuclease that degrades translationally active mRNA molecules. MCPIP1 is mostly known for its anti-inflammatory actions, but it is also an important regulator of adipogenesis and lipid metabolism. Its overexpression impairs adipogenesis by reducing mRNA levels of C/EBPβ and PPARγ, key transcription factors regulating this process. Although adipocytes overexpressing MCPIP1 are characterised by impaired glucose uptake, the function of MCPIP1 in hepatocyte metabolism remains unknown. In this study, conditional deletion of Zc3h12a in murine liver epithelial cells was used to characterise the role of Mcpip1 in adaptation to 24-hour food restriction. We found that Mcpip1 deficiency in liver epithelial cells (Mcpip1fl/flAlbCre mice) resulted in higher blood glucose levels in response to fasting in comparison to Mcpip1fl/fl counterparts. Hepatic proteome analysis showed 26 down-regulated and 117 up-regulated proteins in Mcpip1fl/flAlbCre animals that were involved in cellular adhesion, extracellular matrix and metabolic processes. In conclusion, our studies provide new insight into the hepatic function of Mcpip1 and its involvement in metabolic control.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"919-925"},"PeriodicalIF":1.7,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71476846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel ferroptosis-related gene signature associated with cuproptosis for predicting overall survival in breast cancer patients. 一种与铜中毒相关的新型铁中毒相关基因标记用于预测癌症患者的总生存率。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-06 DOI: 10.18388/abp.2020_6722

Xiaoyu Zhang, Qunchen Zhang

{"title":"A novel ferroptosis-related gene signature associated with cuproptosis for predicting overall survival in breast cancer patients.","authors":"Xiaoyu Zhang, Qunchen Zhang","doi":"10.18388/abp.2020_6722","DOIUrl":"10.18388/abp.2020_6722","url":null,"abstract":"Purpose Ferroptosis and cuproptosis are both metal-dependent regulated cell death that play an important role in cancer. However, the expression patterns and the prognostic values of ferroptosis-related genes (FRGs) associated with cuproptosis in breast cancer (BC) are largely unknown. This study aims to explore the prognostic value of cuproptosis-related FRGs and their relationship with tumor microenvironments in BC. Methods The clinical and RNA sequencing data of BC patients from TCGA, METABRIC and GEO databases were analyzed. The least absolute shrinkage and selection operator regression analysis was used to establish prognostic signatures based on cuproptosis-related FRGs. The overall survival between risk subgroups was assessed by Kaplan-Meier analysis. The changes in risk score during neoadjuvant chemotherapy, and differences in immune cells, immune checkpoints, and drug sensitivity between risk subgroups were also analyzed in this study. Results A successful development of a prognostic signature based on cuproptosis-related FRGs in the TCGA cohort was achieved and it was validated in the METABRIC cohort. Gene set enrichment analysis results revealed the enrichment of steroid biosynthesis and ABC transporters in the high-risk group. Moreover, the signature was also found to be associated with immune cells and immune checkpoints. Lower risk score in patients after neoadjuvant chemotherapy and higher sensitivity of the high-risk group to AKT inhibitor VIII and cisplatin was also observed. Conclusion Cuproptosis-related FRGs can be used as a novel prognostic signature for predicting the overall survival of BC patients. This can provide meaningful insights into the selection of immunotherapy and antitumor drugs for BC.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"875-884"},"PeriodicalIF":1.7,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71476844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simvastatin attenuates diabetes mellitus erectile dysfunction in rats by miR-9-5p-regulated PDCD4. 辛伐他汀通过miR-9-5p调节的PDCD4减轻大鼠糖尿病勃起功能障碍。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-11-06 DOI: 10.18388/abp.2020_6315

YiMing Weng, YuanShen Mao, YanQiu Wang, YuFan Jiao, Jun Xiang, Wei Le

{"title":"Simvastatin attenuates diabetes mellitus erectile dysfunction in rats by miR-9-5p-regulated PDCD4.","authors":"YiMing Weng, YuanShen Mao, YanQiu Wang, YuFan Jiao, Jun Xiang, Wei Le","doi":"10.18388/abp.2020_6315","DOIUrl":"10.18388/abp.2020_6315","url":null,"abstract":"DMED is a common complication of diabetes, for which new treatment methods are urgently required. Focused on DMED, the pharmacological mechanism of simvastatin (Sim) was probed. A model of DMED was made in rats with streptozotocin and orally medicated with Sim. Lentiviral vectors that interfere with miR-9-5p or PDCD4 were injected, and the erectile function, histopathology of cavernous tissue, and α-SMA expression were evaluated. Cavernous smooth muscle cells (CMSCs) obtained from DMED rats were treated with Sim and transfected with the plasmid vector that interferes with miR-9-5p or PDCD4 to observe cell viability and apoptosis. The binding relationship between miR-9-5p and PDCD4 was checked. After 8-week treatment with Sim, erectile function was improved and the corpus cavernosum injury was alleviated. Upregulating miR-9-5p or downregulating PDCD4 further improved erectile function and cavernous injury in rats. miR-9-5p targeted regulation of PDCD4. In vitro cell experiment results showed that Sim induced proliferation and reduced apoptosis of CSMCs by enhancing miR-9-5p-targeted regulating PDCD4 in vitro. Sim attenuates DMED in rats via miR-9-5p/PDCD4.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"791-797"},"PeriodicalIF":1.7,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71476847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele. 儿童骨髓增生症FOLR1和FOLR2基因的突变分析。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-10-26 DOI: 10.18388/abp.2020_6729

Nageen Hussain, Saira Malik, Tayyaba Faiz, Fiza Shafqat, Ayaz Ali Khan, Taqweem Ul Haq, Waqar Ali, Tariq Aziz, Metab Alharbi, Abdulrahman Alshammari, Abdullah F Alasmari

{"title":"Mutational analysis of FOLR1 and FOLR2 genes in children with Myelomeningocele.","authors":"Nageen Hussain, Saira Malik, Tayyaba Faiz, Fiza Shafqat, Ayaz Ali Khan, Taqweem Ul Haq, Waqar Ali, Tariq Aziz, Metab Alharbi, Abdulrahman Alshammari, Abdullah F Alasmari","doi":"10.18388/abp.2020_6729","DOIUrl":"10.18388/abp.2020_6729","url":null,"abstract":"Myelomeningocele (MMC) is a congenital disease. For a long time, molecular mechanism of MMC, the role of folate receptor and transporter proteins remain unclear. Folate from maternal lumen to developing embryo is carried out with the help of folate transporters (SLC46A1, SLC19A1, FOLH1 and SLC25A32) and folate receptor (FOLR1, FOLR2 and FOLR3). Due to the loss of function of these important genes, complications can facilitate the risk of MMC. This study focused on the mutational analysis of FOLR1 and FOLR2 genes in children suffering from MMC. Myelomeningocele is a rare disorder so twenty blood samples from the children were collected. Primers of selected exons for FOLR1 and FOLR2 genes were designed with the help of PrimerFox software. Extracted DNA was amplified, and PCR based mutational analysis was done to check any type of mutation/SNPs in these genes. Sanger sequencing method was performed to confirm mutation in FOLR1 and FOLR2 genes. The results showed that certain environmental factors (smoking, low socio-economic status of mother bearing MMC fetus) were found to be significantly (P<0.05) associated with MMC but no mutation in the selected exons of FOLR1 and FOLR2 genes was detected. Thus, genetic variations in the folate transporter gene may have no role in the progression of MMC in the studied population.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"885-889"},"PeriodicalIF":1.7,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54227281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats. 麻醉和手术诱导老年大鼠内源性脑保护蛋白（RNF146）和谵妄样行为的变化。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-10-26 DOI: 10.18388/abp.2020_6720

Yubo Gao, Xu Han, Xiuhua Li, Shaling Tang, Chun Zhang, Xiaoxia Yang, Majid Alhomrani, Abdulhakeem S Alamri, Ghulam Nabi, Xinli Ni

{"title":"Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats.","authors":"Yubo Gao, Xu Han, Xiuhua Li, Shaling Tang, Chun Zhang, Xiaoxia Yang, Majid Alhomrani, Abdulhakeem S Alamri, Ghulam Nabi, Xinli Ni","doi":"10.18388/abp.2020_6720","DOIUrl":"10.18388/abp.2020_6720","url":null,"abstract":"Background: Postoperative delirium (POD) is a common complication after anesthesia and surgery, especially in the elderly. RNF146 has neuroprotective effects in cerebral ischemia, hypoxia, and chronic neurological diseases. However, whether RNF146 expression is related to the occurrence and development of POD remains unclear. Therefore, in this study, we aimed to determine whether RNF146 is involved in the occurrence of POD.Methods: (Sprague-Dawley) male rats (18 months old) were splenectomized under sevoflurane anesthesia. The cognitive function of rats at 1, 3, and 7 d after anesthesia and surgery was evaluated. Changes in the expression of neuroinflammatory cytokines, IL-6 and IL-10, and RNF146 were measured in the hippocampus in both control group (con) and anesthesia (AS) group. We examined cognitive outcomes and expression of inflammatory factors and RNF146 in con and AS mice using cluster analysis.Results: The cognitive ability and mobility of rats after anesthesia and surgery at day 1, 3, and 7 decreased, especially at day 3. Similarly, the expression of neuroinflammatory factors and RNF146 increased after anesthesia and surgery at day 1, 3, and 7, and the increase was highest at day 3. The clustering and correlation analysis of RNF146 expression in the hippocampi of elderly rats revealed a correlation between POD and neuroinflammation resulting from anesthesia and surgery.Conclusion: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"865-873"},"PeriodicalIF":1.7,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54227280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between EMG high frequency and low frequency band amplitude changes correlates with tissue inorganic phosphate levels. EMG高频和低频带振幅变化之间的关系与组织无机磷酸盐水平相关。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-10-18 DOI: 10.18388/abp.2020_6893

Małgorzata Habich, Bartosz Pawlinski, Kamil Lorenc, Maria Sady, Katarzyna Siewruk, Piotr Zielenkiewicz, Zdzislaw Gajewski, Jaroslaw Poznanski, Leszek Paczek, Pawel Szczesny

{"title":"The relationship between EMG high frequency and low frequency band amplitude changes correlates with tissue inorganic phosphate levels.","authors":"Małgorzata Habich, Bartosz Pawlinski, Kamil Lorenc, Maria Sady, Katarzyna Siewruk, Piotr Zielenkiewicz, Zdzislaw Gajewski, Jaroslaw Poznanski, Leszek Paczek, Pawel Szczesny","doi":"10.18388/abp.2020_6893","DOIUrl":"10.18388/abp.2020_6893","url":null,"abstract":"Assessing inorganic phosphate levels seems crucial in deciphering the biochemical state of organisms or tissues. The concentration of inorganic phosphate in blood is an order of magnitude smaller than in tissues and, on top of that, it is dynamically used to fill temporary gaps in tissues. This is the reason blood inorganic phosphate level is considered a poor proxy for tissue levels. Therefore, tissue biopsy seems to be the dominant method when assessing inorganic phosphate levels for instance in muscles. In this study, we attempted to derive a non-invasive biomarker for phosphate tissue levels. We analyzed surface electromyography signals taken during 31P spectroscopy of leg muscles in five adult pigs. We induced hypophosphatemia via 20 minutes-long hyperventilation. It turned out that the proportion of the amplitude of the low frequency band and the high frequency band is significantly (p=0.002) correlated with the relative phosphate levels. The electromyographic signal did not correlate significantly with pCO2 levels in the blood, suggesting that the changes in the signal are a result of inorganic phosphate levels, not hyperventilation. The results might lead to the development of a real-time phosphate fluctuations measurement procedure.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"951-954"},"PeriodicalIF":1.7,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNA sirtuin-1 restrains the malignant phenotype of non-small cell lung cancer cells via the microRNA-510-5p/SMAD family member 7 axis. 环状RNA sirtuin-1通过微小RNA-510-5p/SMAD家族成员7轴抑制非小细胞肺癌癌症细胞的恶性表型。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-10-18 DOI: 10.18388/abp.2020_6675

ZiRan Zhao, HongYan Zhang, Fan Zhang, Ying Ji, Yue Peng, Fei Wang, Liang Zhao

引用次数: 0

Circular RNA AGFG1 motivates breast cancer cell proliferation, invasion, migration, and glycolysis by controlling microRNA-653-5p/14-3-3 protein epsilon. 环状RNA AGFG1通过控制微小RNA-653-5p/14-3-3蛋白ε来促进癌症细胞增殖、侵袭、迁移和糖酵解。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-10-18 DOI: 10.18388/abp.2020_6254

Liang Chen, JinXian Qian, Ying Shen, Xiang Yu

{"title":"Circular RNA AGFG1 motivates breast cancer cell proliferation, invasion, migration, and glycolysis by controlling microRNA-653-5p/14-3-3 protein epsilon.","authors":"Liang Chen, JinXian Qian, Ying Shen, Xiang Yu","doi":"10.18388/abp.2020_6254","DOIUrl":"10.18388/abp.2020_6254","url":null,"abstract":"A recent Pairwise meta-analysis confirmed that circular RNA AGFG1 (circAGFG1) is abnormally highly expressed in breast cancer (BC) and may be associated with death risk. The purpose of this study was to elucidate the biological role of circAGFG1 in BC and to explore its potential downstream molecular mechanisms. CircAGFG1, miR-653-5p and YWHAE expression in BC tissues and cells were analyzed by RT-qPCR or western blot. Gene expression was regulated by transfection of plasmids or oligonucleotides and the biological behaviors of BC cells were analyzed by a series of assays. The ring structure of circAGFG1 was analyzed by RNase R and actinomycin D treatment. Dual luciferase reporter assay and RNA-pull down were used to verify the targeting relationship of circAGFG1 and downstream factors. A nude mouse xenograft experiment was performed to verify the effect of circAGFG1 on cancer cells in vivo. The results showed that circAGFG1 and YWHAE were highly expressed in BC while miR-653-5p was lowly expressed. Both circAGFG1 and YWHAE had a targeting relationship with miR-653-5p. Knockdown of circAGFG1 inhibited BC cell proliferation, invasion, migration, and glycolysis. The inhibitory effect of circAGFG1 knockdown on BC was reversed by silencing miR-653-5p. The inhibitory effect of overexpression of miR-653-5p on malignant behaviors of BC cells was reversed by overexpression of YWHAE. Knockdown of circAGFG1 inhibited tumor growth in vivo. Taken together, these data suggest that circAGFG1 acts as a sponge for miR-653-5p to mediate YWHAE expression to promote the malignant behaviors of BC.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"777-784"},"PeriodicalIF":1.7,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LINC00707 promotes multidrug resistance of ovarian cancer cells by targeting the miR-382-5p/LRRK2 axis. LINC00707通过靶向miR-382-5p/LRRK2轴来促进卵巢癌症细胞的多药耐药性。

IF 1.7 4区生物学

Acta biochimica Polonica Pub Date : 2023-10-03 DOI: 10.18388/abp.2020_6503

Min-Wen Zhao, Chang-Jie Lin, Jian-Ping Qiu

{"title":"LINC00707 promotes multidrug resistance of ovarian cancer cells by targeting the miR-382-5p/LRRK2 axis.","authors":"Min-Wen Zhao, Chang-Jie Lin, Jian-Ping Qiu","doi":"10.18388/abp.2020_6503","DOIUrl":"10.18388/abp.2020_6503","url":null,"abstract":"Multidrug resistance severely limits the efficacy of ovarian cancer (OC) treatment. Recent studies have revealed the carcinogenic role of LINC00707 RNA. However, the role of LINC00707 in the development of multidrug resistance in OC has not been clarified. Therefore, the aim of this study was to investigate the relationship between LINC00707 and multidrug resistance in OC, which can facilitate the development of new therapeutic agents for effectively addressing this issue. The RNA expression of LINC00707, miR-382-5p and leucine-rich repeat kinase 2 (LRRK2) in SKOV3 (a human OC cell line) cells was detected by qRT-PCR. The effects of LINC00707 on the proliferation and viability of SKOV3 cells were determined by MTT assay and colony formation assay. The interaction of LINC00707, miR-382-5p, and LRRK2 was bioinformatically predicted and verified with dual-luciferase reporter assay. In addition, the effect of LINC00707 on drug resistance in SKOV3 cells through targeting the miR-382-5p/LRRK2 axis was explored. The expression of LINC00707 and LRRK2 was significantly increased in SKOV3 cells, while miR-382-5p expression was significantly decreased. The results of bioinformatic prediction and colony formation assay demonstrated that LINC00707 could regulate LRRK2 expression in SKOV3 cells by targeting miR-382-5p. Additionally, knockdown of LINC00707 markedly increased expression of miR-382-5p and decreased that of LRRK2, increased cell proliferation and viability, as well as sensitivity to chemotherapeutic agents in SKOV3 cells. Notably, these manifestations were more obvious with simultaneous knockdown of LINC00707 and miR-382-5p compared with knockdown of LINC00707 alone. LINC00707 is overexpressed in SKOV3 cells and promotes SKOV3 cell proliferation and resistance to chemotherapeutic drugs via targeting the miR-382-5p/LRRK2 axis.","PeriodicalId":6984,"journal":{"name":"Acta biochimica Polonica","volume":" ","pages":"799-806"},"PeriodicalIF":1.7,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41106968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0