Yigeng Feng, Hongwen Cao, Dan Wang, Lei Chen, Renjie Gao, Peng Sun
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引用次数: 0
摘要
背景：前列腺癌是全球最常见的男性癌症之一：前列腺癌是全球男性最常见的癌症之一。本研究旨在阐明 c-Jun N 端激酶（JNK）在去势抵抗性前列腺癌（CRPC）进展过程中的作用：方法：在PC-3前列腺癌细胞系上建立JNK过表达和敲除细胞系。MTT 和 Matrigel 侵袭试验分别用于评估细胞活力和侵袭能力。采用 Kaplan-Meier 估计器估算总生存率和第二次无进展生存率。皮尔逊相关系数用于评估JNK与前列腺特异性抗原（PSA）之间的关系：结果：JNK的相对表达与CRPC患者的Gleason评分和PSA值相关。Kaplan-Meier分析显示，JNK表达量低的患者总生存率高，无进展生存率次之。体外实验表明，JNK的过度表达促进了PC-3细胞系的细胞活力和侵袭能力，以及细胞外信号调节激酶（ERK）和基质金属蛋白酶1（MMP1）的蛋白表达：结论：JNK过表达通过调控ERK和MMP1促进CRPC的发展。
JNK promotes the progression of castration-resistant prostate cancer.
Background: Prostate cancer is one of the most common cancers in men worldwide. This study aims to elucidate the roles of c-Jun N-terminal kinase (JNK) in the progression of castration-resistant prostate cancer (CRPC).
Methods: JNK overexpressing and knockdown cell lines were established on the PC-3 prostate cell line. qPCR and Western blotting were performed to determine the mRNA and protein levels of target genes in prostate tissues and cell lines. MTT and Matrigel invasion assays were conducted to evaluate the cell viability and invasive ability, respectively. The Kaplan-Meier estimator was performed to estimate the overall survival rate and second progression-free survival rate. Pearson's correlation coefficient was used to evaluate the relationship between JNK and prostate-specific antigen (PSA).
Results: Relative JNK expression was correlated with Gleason score and PSA value in patients with CRPC. Kaplan-Meier analysis revealed that patients with low JNK expression exhibited high overall survival and second progression-free survival rate. In vitro assays demonstrated that JNK overexpression promoted cell viability and invasion as well as the protein expressions of extracellular signal-regulated kinase (ERK) and matrix metalloproteinase 1 (MMP1) in PC-3 cell lines.
Conclusions: JNK overexpression promotes the development of CRPC via the regulation of ERK and MMP1.
期刊介绍：
Acta Biochimica Polonica is a journal covering enzymology and metabolism, membranes and bioenergetics, gene structure and expression, protein, nucleic acid and carbohydrate structure and metabolism.
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