Evolutionary Applications最新文献

{"title":"Geographic Range Size Predicts Butterfly Species' Tolerance to Heavy Metals More Than Evolutionary History With Toxic Larval Diets","authors":"Ashley L. Darst,&nbsp;Lindsey R. Kemmerling,&nbsp;Molly Tilsen,&nbsp;J. Alexander Eilts,&nbsp;Emilie C. Snell-Rood","doi":"10.1111/eva.70114","DOIUrl":"https://doi.org/10.1111/eva.70114","url":null,"abstract":"<p>Some organisms appear to thrive in contaminated environments, while others are more sensitive, though the causes of this variation are unclear. The toxin coevolution hypothesis posits that an evolutionary history with natural toxins preadapts species to deal with novel toxins, while the range-size-tolerance hypothesis posits that a larger geographic range selects for broader tolerance to stressors. Butterflies are a prime system to investigate these hypotheses because they are diverse, feed on a range of larval host plants that vary in defensive compounds, and many are found in polluted environments. We ask how these hypotheses explain varying tolerance to heavy metal pollution, measured here as loads of four heavy metals along an urban gradient of metal exposure. We compared 26 butterfly species that vary in their evolutionary history with mutagenic plant defensive chemicals as well as their geographic range size. We built a dataset of plant mutagenicity synthesizing 40 years of standardized mutagenicity screening in plants, including 502 plant species of 103 families within 37 orders. We used this dataset, coupled with butterfly host records, to estimate evolutionary history with mutagens. We found that butterfly species with larger ranges tolerated significantly greater concentrations of lead, arsenic, and cadmium in their tissues. Additionally, species with a history of feeding on relatively more mutagenic host plant families tolerated greater maximum lead concentrations in their thoracic tissue. This research provides additional support for the growing observation that small-ranged species are more vulnerable to environmental change, in this case, metal pollution. In addition, an evolutionary history with mutagenic host plants may provide some additional resilience, although less than geographic range size. In addition, our dataset on comparative plant mutagenicity will facilitate future research on plant-herbivore coevolution, in fields such as chemical, community, and urban ecology.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical Cancer Transmission via Asexual Fragmentation and Associated Cancer Prevalence","authors":"Jibeom Choi","doi":"10.1111/eva.70111","DOIUrl":"https://doi.org/10.1111/eva.70111","url":null,"abstract":"<p>While sexual reproduction is a general feature of animals, fissiparity and budding are relatively uncommon modes of asexual reproduction by which a fragment from a parent becomes an independent organism. Unlike unitary development, tumor cells can be included in the detached fragment destined to become offspring. Although fragmentation facilitates the vertical transmission of parental tumor cells to nascent progeny, this process requires significantly fewer cell replications than development from a zygote. The former is a risk factor for cancer, while the latter reduces oncogenic mutations during replication, indicating that two opposite effects of carcinogenesis are involved in fragmentation. If fragmentation can significantly reduce the number of cell replications for the development and a small portion of parental cancer is transmitted to the offspring during fragmentation, consecutive fragmentation across generations can gradually diminish the cancer risk of offspring, which I term fragmentational purging. On the other hand, consecutive fragmentation may aggravate the cancer risk of the progeny, a process of fragmentational accumulation. The model results imply that fragmentational purging does not necessarily guarantee the evolution of fragmentation, nor does fragmentational accumulation ensure its exclusion. Other relevant factors including juvenile susceptibility of sexual reproduction and loss of genetic diversity stemming from asexual reproduction can influence the selective advantage of fragmentation. Furthermore, owing to the common features of stemness and self-renewal, the existence of pluripotent adult stem cells required for fragmentation could be coupled with elevated cancer risk. The model results across diverse parameters and the associated mathematical analyses highlight multifaceted evolutionary trajectories toward fragmentation. Further investigation of cancer-suppression strategies that fragmentational animals employ could provide insights into regenerative medicine and cancer therapy.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color","authors":"Adam F. Parlin,&nbsp;Bradley J. Cosentino,&nbsp;Richard M. Lehtinen,&nbsp;John E. McDonald,&nbsp;Emma C. C. Sinclair,&nbsp;James P. Gibbs","doi":"10.1111/eva.70109","DOIUrl":"https://doi.org/10.1111/eva.70109","url":null,"abstract":"<p>Cities impose unique selection pressures on wildlife and generate clines in phenotypic traits along urban–rural gradients. Roads are a widespread feature of human-dominated landscapes and are known to cause direct wildlife mortality; however, whether they act as a selective force influencing phenotypic trait variation along urban–rural gradients remains unclear. This study tested the hypothesis that roads influence natural selection of coat color in the eastern gray squirrel (<i>Sciurus carolinensis</i>), a species with two distinct coat colors: a gray morph that is common in all areas and a melanic morph more prevalent in urban areas than in rural ones. Vehicular collisions are a significant cause of mortality in eastern gray squirrels, with the melanic morph more visually conspicuous on roads and more easily detected and avoided by drivers than the gray morph. Standardized road cruise surveys along an urbanization gradient in Syracuse, New York, USA, revealed that the prevalence of melanism among living squirrels in Syracuse was negatively related to distance from the city center, whereas there was no urban–rural cline in melanism among road-killed individuals, with the melanic morph underrepresented among road-killed squirrels by up to 30% along the urbanization gradient. An examination of the prevalence of each color morph on and off road surfaces in a range-wide compilation of &gt; 100,000 photographs of <i>S. carolinensis</i> also indicated that the melanic morph was underrepresented among road-killed squirrels imaged. Our study highlights vehicular collisions as an important source of natural selection on phenotypic traits, suggesting a potential role in shaping patterns of urban evolution and contributing to the maintenance of urban–rural clines.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Stressors in the Anthropocene: Urban Evolutionary History Modifies Sensitivity to the Toxic Effects of Crude Oil Exposure in Killifish","authors":"Jane Park,&nbsp;Charles Brown,&nbsp;Chelsea Hess,&nbsp;Madison Armstrong,&nbsp;David M. Rocke,&nbsp;Fernando Galvez,&nbsp;Andrew Whitehead","doi":"10.1111/eva.70112","DOIUrl":"https://doi.org/10.1111/eva.70112","url":null,"abstract":"<p>Persistence of wild species in human-altered environments is difficult, in part because challenges to fitness are complex when multiple environmental changes occur simultaneously, which is common in the Anthropocene. This complexity is difficult to conceptualize because the nature of environmental change is often highly context specific. A mechanism-guided approach may help to shape intuition and predictions about complexity; fitness challenges posed by co-occurring stressors with similar mechanisms of action may be less severe than for those with different mechanisms of action. We approach these considerations within the context of ecotoxicology because this field is built upon a rich mechanistic foundation. We hypothesized that evolved resistance to one class of common toxicants would afford resilience to the fitness impacts of another class of common toxicants that shares mechanisms of toxicity. <i>Fundulus</i> killifish populations in urban estuaries have repeatedly evolved resistance to persistent organic pollutants including PCBs. Since PCBs and some of the toxicants that constitute crude oil (e.g., high molecular weight PAHs) exert toxicity through perturbation of AHR signaling, we predicted that PCB-resistant populations would also be resilient to crude oil toxicity. Common garden comparative oil exposure experiments, including killifish populations with different exposure histories, showed that most killifish populations were sensitive to fitness impacts (reproduction and development) caused by oil exposure, but that fish from the PCB-resistant population were insensitive. Population differences in toxic outcomes were not compatible with random-neutral expectations. Transcriptomics revealed that the molecular mechanisms that contributed to population variation in PAH resilience were shared with those that contribute to evolved variation in PCB resilience. We conclude that the fitness challenge posed by environmental pollutants is effectively reduced when those chemicals share mechanisms that affect fitness. Mechanistic considerations may help to scale predictions regarding the fitness challenges posed by stressors that may co-occur in human-altered environments.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local Adaptation and Climate Change Vulnerability of the Relict Tree Species Taiwania cryptomerioides Provide Insights Into Its Conservation and Restoration","authors":"Yang Lu,&nbsp;Hao Dong,&nbsp;Saibin Fan,&nbsp;Lu Yuan,&nbsp;Yuhui Wang,&nbsp;Zhuang Zhao,&nbsp;Yong Lai,&nbsp;Shixin Zhu,&nbsp;Jinyong Huang,&nbsp;Caipeng Yue,&nbsp;Yongpeng Ma,&nbsp;Ningning Zhang","doi":"10.1111/eva.70113","DOIUrl":"https://doi.org/10.1111/eva.70113","url":null,"abstract":"<p>Rapid climate change is affecting biodiversity and threatening locally adapted species. Relict species are often confined to relatively narrow, discontinuous geographic ranges and provide excellent opportunities to study local adaptation and extinction. Understanding the adaptive genetic variation and genetic vulnerability of relict species under climate change is essential for their conservation and management efforts. Here, we applied a landscape genomics approach to investigate the population genetic structure and predict adaptive capacity to climatic change for <i>Taiwania cryptomerioides</i> Hayata, a vulnerable Tertiary relict tree species in China. We used restriction site-associated DNA sequencing on 122 individuals across 10 sampling sites. We found three genetic groups across the Chinese range of <i>T. cryptomerioides</i>: the southwest, central-eastern, and Taiwanese groups. We detected significant signals of isolation by environment and isolation by distance, with environment playing a more important role than geography in shaping spatial genetic variation in <i>T. cryptomerioides</i>. Moreover, some outliers were related to defense and stress responses, which could reflect the genomic basis of adaptation. Gradient forest (GF) analysis revealed that precipitation-related variables were important in driving adaptive variation in <i>T. cryptomerioides</i>. Ecological niche modeling and GF analysis revealed that the central-eastern populations were more vulnerable to future climate change than other populations, with range contractions and high genetic offsets, suggesting these populations may be at higher risk of decline or local extinction. These findings deepen our understanding of local adaptation and vulnerability to climate change in relict tree species and will guide conservation and restoration programs for <i>T. cryptomerioides</i> in the future.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Guide for Developing Demo-Genetic Models to Simulate Genetic Rescue","authors":"Julian E. Beaman,&nbsp;Katie Gates,&nbsp;Frédérik Saltré,&nbsp;Carolyn J. Hogg,&nbsp;Katherine Belov,&nbsp;Kita Ashman,&nbsp;Karen Burke da Silva,&nbsp;Luciano B. Beheregaray,&nbsp;Corey J. A. Bradshaw","doi":"10.1111/eva.70092","DOIUrl":"https://doi.org/10.1111/eva.70092","url":null,"abstract":"<p>Genetic rescue is a conservation management strategy that reduces the negative effects of genetic drift and inbreeding in small and isolated populations. However, such populations might already be vulnerable to random fluctuations in growth rates (demographic stochasticity). Therefore, the success of genetic rescue depends not only on the genetic composition of the source and target populations but also on the emergent outcome of interacting demographic processes and other stochastic events. Developing predictive models that account for feedback between demographic and genetic processes (‘demo-genetic feedback’) is therefore necessary to guide the implementation of genetic rescue to minimize the risk of extinction of threatened populations. Here, we explain how the mutual reinforcement of genetic drift, inbreeding, and demographic stochasticity increases extinction risk in small populations. We then describe how these processes can be modelled by parameterizing underlying mechanisms, including deleterious mutations with partial dominance and demographic rates with variances that increase as abundance declines. We combine our suggestions of model parameterization with a comparison of the relevant capability and flexibility of five open-source programs designed for building genetically explicit, individual-based simulations. Using one of the programs, we provide a heuristic model to demonstrate that simulated genetic rescue can delay extinction of small virtual populations that would otherwise be exposed to greater extinction risk due to demo-genetic feedback. We then use a case study of threatened Australian marsupials to demonstrate that published genetic data can be used in one or all stages of model development and application, including parameterization, calibration, and validation. We highlight that genetic rescue can be simulated with either virtual or empirical sequence variation (or a hybrid approach) and suggest that model-based decision-making should be informed by ranking the sensitivity of predicted probability/time to extinction to variation in model parameters (e.g., translocation size, frequency, source populations) among different genetic-rescue scenarios.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multipurpose Microhaplotype Panel for Genetic Analysis of California Chinook Salmon","authors":"Eric C. Anderson,&nbsp;Anthony J. Clemento,&nbsp;Matthew A. Campbell,&nbsp;Devon E. Pearse,&nbsp;Anne K. Beulke,&nbsp;Cassie Columbus,&nbsp;Ellen Campbell,&nbsp;Neil F. Thompson,&nbsp;John Carlos Garza","doi":"10.1111/eva.70110","DOIUrl":"https://doi.org/10.1111/eva.70110","url":null,"abstract":"<p>Genetic methods have become an essential component of ecological investigation and conservation planning for fish and wildlife. Among these methods is the use of genetic marker data to identify individuals to populations, or stocks, of origin. More recently, methods that involve genetic pedigree reconstruction to identify relationships between individuals within populations have also become common. We present here a novel set of multiallelic microhaplotype genetic markers for Chinook salmon, which provide excellent resolution for population discrimination and relationship identification from a rapidly and economically assayed panel of markers. We show how this set of genetic markers assayed by sequencing 204 amplicons, in tandem with a reference dataset of 1636 individual samples from 17 populations, provides definitive power to identify all known lineages of Chinook salmon in California. The inclusion of genetic loci that have known associations with phenotype and that were identified as outliers in examination of whole-genome sequence data allows resolution of stocks that are not highly genetically differentiated but are phenotypically distinct and managed as such. This same set of multiallelic genetic markers has ample variation to accurately identify parent-offspring and full-sibling pairs in all California populations, including the genetically depauperate winter-run lineage. Validation of this marker panel in coastal salmon populations not previously studied with modern genetic methods also reveals novel biological insights, including the presence of a single copy of a haplotype for a phenotype that has not been documented in that part of the species range, and a clear signal of mixed ancestry for a salmon population that is on the geographic margins of the primary evolutionary lineages present in California.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Signatures of Selection for Resistance/Tolerance to Perkinsus olseni in Grooved Carpet Shell Clam (Ruditapes decussatus) Using a Population Genomics Approach","authors":"Inés M. Sambade,&nbsp;João Estêvão,&nbsp;Marina Pampín,&nbsp;Andreia Cruz,&nbsp;Eric Guévélou,&nbsp;Andrés Blanco,&nbsp;Francisco Câmara,&nbsp;Jessica Gómez-Garrido,&nbsp;Fernando Cruz,&nbsp;Luca Bargelloni,&nbsp;Stefano Carboni,&nbsp;Tyler Alioto,&nbsp;Benjamin Costas,&nbsp;Sergio Fernández-Boo,&nbsp;Paulino Martínez","doi":"10.1111/eva.70106","DOIUrl":"https://doi.org/10.1111/eva.70106","url":null,"abstract":"<p>The grooved carpet shell clam (<i>Ruditapes decussatus</i>) is a bivalve of high commercial value distributed throughout the European coast. Its production has suffered a decline caused by different factors, especially by the parasite <i>Perkinsus olsenii</i>. Improving production of <i>R</i>. <i>decussatus</i> requires genomic resources to ascertain the genetic factors underlying resistance/tolerance to <i>P. olsenii</i>. In this study, the first reference genome of <i>R</i>. <i>decussatus</i> was assembled through long- and short-read sequencing (1677 contigs; 1.386 Mb) and further scaffolded at chromosome level with Hi-C (19 superscaffolds; 95.4% of assembly). Repetitive elements were identified (32%) and masked for annotation of 38,276 coding- and 13,056 non-coding genes. This genome was used as a reference to develop a 2bRAD-Seq 13,438 SNP panel for a genomic screening on six shellfish beds distributed across the Atlantic Ocean and Mediterranean Sea. Beds were selected by perkinsosis prevalence and the infection level was individually evaluated in all the samples. Genetic diversity was significantly higher in the Mediterranean than in the Atlantic region. The main genetic breakage was detected between those regions (F_ST = 0.224), being the Mediterranean more heterogeneous than the Atlantic. Several loci under divergent selection (394 outliers; 261 genomic windows) were detected across shellfish beds. Samples were also inspected to detect signals of selection for resistance/tolerance to <i>P. olsenii</i> by using infection-level and population-genomics approaches, and 90 common divergent outliers for resistance/tolerance to perkinsosis were identified and used for gene mining. Candidate genes and markers identified provide invaluable information for controlling perkinsosis and for improving production of the grooved carpet shell clam.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Beyond Karstification Have Shaped the Population Structure of a Surface-Dwelling Minnow (Phoxinus lumaireul) Able to Disperse Underground","authors":"Susanne Reier,&nbsp;Peter Trontelj,&nbsp;Luise Kruckenhauser,&nbsp;Martin Kapun,&nbsp;Alexandra Wanka,&nbsp;Anja Palandačić","doi":"10.1111/eva.70104","DOIUrl":"https://doi.org/10.1111/eva.70104","url":null,"abstract":"<p>The Dinaric Karst, a biodiversity hotspot, features complex surface and subterranean hydrological networks that influence aquatic species distribution. This study investigates how karst hydrology shapes the genetic structure of the surface-dwelling minnow <i>Phoxinus lumaireul</i>, examining both large-scale and small-scale population patterns. Using mitochondrial DNA and genome-wide single nucleotide polymorphism (SNP) data of 827 specimens of <i>P. lumaireul</i>, three hypotheses were tested: (1) karst underground water connections facilitate genetic connectivity within and across river systems, whereas non-karst rivers exhibit genetic connectivity mostly within the same system; (2) historical and occasional hydrological connections have shaped present-day population structure, leaving genetic signatures of relatedness where no contemporary hydrological links exist; and (3) genomic approaches provide additional insights into biologically relevant connections that may not be captured by classical tracing tests. The large-scale analyses confirmed three main genetic groups (1a–c), whose structure was likely shaped by Pleistocene glaciations and associated microrefugia rather than by karst hydrology. Small-scale structure analyses revealed that while karst hydrology facilitated gene flow within specific areas, connectivity was uneven and influenced by local hydrological dynamics and historical admixture events. Furthermore, some underground pathways identified by classical tracing tests lacked evidence of genetic connectivity, underscoring the limitations of traditional methods and the added value of genomic data in indirectly detecting biologically relevant hydrological connections. These findings highlight the influence of both historical processes and contemporary karst hydrology on <i>P. lumaireul</i> populations, emphasizing their vulnerability in karst ecosystems and the need for targeted conservation efforts.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Patterns of Raccoon (Procyon lotor) Spatial Population Genomics Throughout a Rabies Management Area in Eastern North America","authors":"Matthew W. Hopken,&nbsp;Clara P. Mankowski,&nbsp;Christine Thurber,&nbsp;Antoinette J. Piaggio,&nbsp;Kathleen M. Nelson,&nbsp;Richard B. Chipman,&nbsp;Zaid Abdo,&nbsp;Tore Buchanan,&nbsp;Ariane Massé,&nbsp;Amy T. Gilbert","doi":"10.1111/eva.70105","DOIUrl":"https://doi.org/10.1111/eva.70105","url":null,"abstract":"<p>Wide-ranging, generalist species provide both interesting and challenging opportunities for research questions focused on population structure. Their continuous distributions and ability to occupy diverse habitat types can obscure genetic signals of ancestry and geographic clustering. However, spatially informed population genetic approaches are notable for high-resolution identification of geographic clusters that often elude more classical clustering models. The northern raccoon (<i>Procyon lotor</i>) is a broadly distributed species in North America, with populations in diverse habitats ranging from dense urban to rural landscapes. Wildlife management agencies have an interest in understanding raccoon ecology, given their propensity for human-wildlife conflicts and zoonotic diseases. We combined samples from an extensive raccoon tissue repository with a RADcapture panel of 1000 microhaplotype loci to conduct spatial genetic analyses of raccoon populations in eastern North America. Our objective was to estimate patterns of genetic diversity on the landscape that may inform raccoon rabies management. Bayesian clustering analyses delineated multiple ancestry clusters that encompassed large areas across 22 US states and 2 Canadian provinces. We discovered a potential phylogeographic split between central and southern samples from those in the northeast region, which correlates with post-Pleistocene recolonization detected in a multitude of species from the region. A finer scale structure was identified using spatially explicit analyses and demonstrated variable dispersal/gene flow patterns within specific regions. The Appalachian Mountain region restricted local connectivity among raccoons, while raccoon populations in central New York, the Ohio River Valley, southern Québec, and southern Alabama demonstrated high genetic connectivity. The results from this study highlight how raccoon ecology and historical biogeography can help contextualize contrasting hypotheses about the influence of landscape on raccoon movement patterns, which can inform management of zoonotic disease risks at regional scales.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}