Evolutionary Applications最新文献

{"title":"Chemical mutagenesis and thermal selection of coral photosymbionts induce adaptation to heat stress with trait trade-offs","authors":"Hugo J. Scharfenstein,&nbsp;Carlos Alvarez-Roa,&nbsp;Lesa M. Peplow,&nbsp;Patrick Buerger,&nbsp;Wing Yan Chan,&nbsp;Madeleine J. H. van Oppen","doi":"10.1111/eva.13586","DOIUrl":"https://doi.org/10.1111/eva.13586","url":null,"abstract":"<p>Despite the relevance of heat-evolved microalgal endosymbionts to coral reef restoration, to date, few Symbiodiniaceae strains have been thermally enhanced via experimental evolution. Here, we investigated whether the thermal tolerance of Symbiodiniaceae can be increased through chemical mutagenesis followed by thermal selection. Strains of <i>Durusdinium trenchii</i>, <i>Fugacium kawagutii</i> and <i>Symbiodinium pilosum</i> were exposed to ethyl methanesulfonate to induce random mutagenesis, and then underwent thermal selection at high temperature (31/33°C). After 4.6–5 years of experimental evolution, the in vitro thermal tolerance of these strains was assessed via reciprocal transplant experiments to ambient (27°C) and elevated (31/35°C) temperatures. Growth, photosynthetic efficiency, oxidative stress and nutrient use were measured to compare thermal tolerance between strains. Heat-evolved <i>D. trenchii</i>, <i>F. kawagutii</i> and <i>S. pilosum</i> strains all exhibited increased photosynthetic efficiency under thermal stress. However, trade-offs in growth rates were observed for the heat-evolved <i>D. trenchii</i> lineage at both ambient and elevated temperatures. Reduced phosphate and nitrate uptake rates in <i>F. kawagutii</i> and <i>S. pilosum</i> heat-evolved lineages, respectively, suggest alterations in nutrition resource usage and allocation processes may have occurred. Increased phosphate uptake rates of the heat-evolved <i>D. trenchii</i> strain indicate that experimental evolution resulted in further trade-offs in this species. These findings deepen our understanding of the physiological responses of Symbiodiniaceae cultures to thermal selection and their capacity to adapt to elevated temperatures. The new heat-evolved Symbiodiniaceae developed here may be beneficial for coral reef restoration efforts if their enhanced thermal tolerance can be conferred <i>in hospite</i>.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 9","pages":"1549-1567"},"PeriodicalIF":4.1,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41081630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide diversity evaluation and core germplasm extraction in ex situ conservation: A case of golden Camellia tunghinensis","authors":"Xianliang Zhu,&nbsp;Rong Zou,&nbsp;Huizhen Qin,&nbsp;Shengfeng Chai,&nbsp;Jianmin Tang,&nbsp;Yingying Li,&nbsp;Xiao Wei","doi":"10.1111/eva.13584","DOIUrl":"https://doi.org/10.1111/eva.13584","url":null,"abstract":"<p>Whether ex situ populations constructed in the limited nursery resources of botanical gardens can preserve enough genetic diversity of endangered plants in the wild remains uncertain. Here, a case study was conducted with <i>Camellia tunghinensis</i>, which is one of the species with the lowest natural distribution area in the sect. <i>Chrysantha</i> (golden camellia) of the family Theaceae. We investigated the genetic diversity and population structure of 229 samples from wild and ex situ populations using genotyping by sequencing (GBS). Core germplasm was constructed from these samples. The results showed that wild <i>C. tunghinensis</i> exhibited high genetic diversity, with observed heterozygosity of 0.257–0.293 and expected heterozygosity of 0.247–0.262. Compared with wild populations, the genetic diversity of ex situ populations established by transplanting wild seedlings was close to or even higher. However, the genetic diversity of those established by seed or cuttings of a few superior trees was lower. The Admixture analysis revealed that the structure of the ex situ populations derived from seeds and cuttings was relatively simple compared with the ex situ populations derived from transplanted wild seedlings and wild populations. These results suggested that direct transplanting of wild seedlings was more conducive to preserving the genetic diversity of endangered plants in the wild. In addition, wild populations demonstrated a small differentiation (mean <i>F</i>_ST = 0.044) among themselves, possibly due to long-term and frequent gene flow between the wild populations. In contrast, moderate differentiation (mean <i>F</i>_ST &gt; 0.05) was detected among ex situ populations and between ex situ and wild populations. This may be the combined result of the absence of gene flow pathways and strong selection pressure in various ex situ environments. Finally, 77 core germplasms were extracted from 229, likely representing the genetic diversity of <i>C. tunghinensis</i>. This study provides future strategies for the ex situ conservation and management of the golden camellia species and other rare and endangered plants.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 9","pages":"1519-1530"},"PeriodicalIF":4.1,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13584","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41081631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different mechanisms underpin the decline in growth of anchovies and sardines of the Bay of Biscay","authors":"Andy Bo?ns,&nbsp;Bruno Ernande,&nbsp;Pierre Petitgas,&nbsp;Christophe Lebigre","doi":"10.1111/eva.13564","DOIUrl":"https://doi.org/10.1111/eva.13564","url":null,"abstract":"<p>Declines in individuals' growth in exploited fish species are generally attributed to evolutionary consequences of size-selective fishing or to plastic responses due to constraints set by changing environmental conditions dampening individuals' growth. However, other processes such as growth compensation and non-directional selection can occur and their importance on the overall phenotypic response of exploited populations has largely been ignored. Using otolith growth data collected in European anchovy and sardine of the Bay of Biscay (18 cohorts from 2000 to 2018), we parameterized the breeder's equation to determine whether declines in size-at-age in these species were due to an adaptive response (i.e. related to directional or non-directional selection differentials within parental cohorts) or a plastic response (i.e. related to changes in environmental). We found that growth at age-0 in anchovy declined between parents and their offspring when biomass increased and the selective disappearance of large individuals was high in parents. Therefore, an adaptive response probably occurred in years with high fishing effort and the large increase in biomass after the collapse of this stock maintained this adaptive response subsequently. In sardine offspring, higher growth at age-0 was associated with increasing biomass between parents and offspring, suggesting a plastic response to a bottom-up process (i.e. a change in food quantity or quality). Parental cohorts in which selection favoured individuals with high growth compensation produced offspring high catch up growth rates, which may explain the smaller decline in growth in sardine relative to anchovy. Finally, on non-directional selection differentials were not significantly related to the changes in growth at age-0 and growth compensation at age-1 in both species. Although anchovy and sardine have similar ecologies, the mechanisms underlying the declines in their growth are clearly different. The consequences of the exploitation of natural populations could be long lasting if density-dependent processes follow adaptive changes.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1393-1411"},"PeriodicalIF":4.1,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13564","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6163878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population demographic history and evolutionary rescue: Influence of a bottleneck event","authors":"Laure Olazcuaga,&nbsp;Beatrice Lincke,&nbsp;Sarah DeLacey,&nbsp;Lily F. Durkee,&nbsp;Brett A. Melbourne,&nbsp;Ruth A. Hufbauer","doi":"10.1111/eva.13581","DOIUrl":"https://doi.org/10.1111/eva.13581","url":null,"abstract":"<p>Rapid environmental change presents a significant challenge to the persistence of natural populations. Rapid adaptation that increases population growth, enabling populations that declined following severe environmental change to grow and avoid extinction, is called evolutionary rescue. Numerous studies have shown that evolutionary rescue can indeed prevent extinction. Here, we extend those results by considering the demographic history of populations. To evaluate how demographic history influences evolutionary rescue, we created 80 populations of red flour beetle, <i>Tribolium castaneum</i>, with three classes of demographic history: diverse populations that did not experience a bottleneck, and populations that experienced either an intermediate or a strong bottleneck. We subjected these populations to a new and challenging environment for six discrete generations and tracked extinction and population size. Populations that did not experience a bottleneck in their demographic history avoided extinction entirely, while more than 20% of populations that experienced an intermediate or strong bottleneck went extinct. Similarly, among the extant populations at the end of the experiment, adaptation increased the growth rate in the novel environment the most for populations that had not experienced a bottleneck in their history. Taken together, these results highlight the importance of considering the demographic history of populations to make useful and effective conservation decisions and management strategies for populations experiencing environmental change that pushes them toward extinction.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1483-1495"},"PeriodicalIF":4.1,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13581","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6098685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latitude-specific urbanization effects on life history traits in the damselfly Ischnura elegans","authors":"Gemma Palomar,&nbsp;Guillaume Wos,&nbsp;Robby Stoks,&nbsp;Szymon Sniegula","doi":"10.1111/eva.13583","DOIUrl":"https://doi.org/10.1111/eva.13583","url":null,"abstract":"<p>Many species are currently adapting to cities at different latitudes. Adaptation to urbanization may require eco-evolutionary changes in response to temperature and invasive species that may differ between latitudes. Here, we studied single and combined effects of increased temperatures and an invasive alien predator on the phenotypic response of replicated urban and rural populations of the damselfly <i>Ischnura elegans</i> and contrasted these between central and high latitudes. Adult females were collected in rural and urban ponds at central and high latitudes. Their larvae were exposed to temperature treatments (current [20°C], mild warming [24°C], and heat wave [28°C; for high latitude only]) crossed with the presence or absence of chemical cues released by the spiny-cheek crayfish (<i>Faxonius limosus</i>), only present at the central latitude. We measured treatment effects on larval development time, mass, and growth rate. Urbanization type affected all life history traits, yet these responses were often dependent on latitude, temperature, and sex. Mild warming decreased mass in rural and increased growth rate in urban populations. The effects of urbanization type on mass were latitude-dependent, with central-latitude populations having a greater phenotypic difference. Urbanization type effects were sex-specific with urban males being lighter and having a lower growth rate than rural males. At the current temperature and mild warming, the predator cue reduced the growth rate, and this independently of urbanization type and latitude of origin. This pattern was reversed during a heat wave in high-latitude damselflies. Our results highlight the context-dependency of evolutionary and plastic responses to urbanization, and caution for generalizing how populations respond to cities based on populations at a single latitude.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1503-1515"},"PeriodicalIF":4.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6067491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calibrating epigenetic clocks with training data error","authors":"Benjamin Mayne,&nbsp;Oliver Berry,&nbsp;Simon Jarman","doi":"10.1111/eva.13582","DOIUrl":"https://doi.org/10.1111/eva.13582","url":null,"abstract":"<p>Animal age data are valuable for management of wildlife populations. Yet, for most species, there is no practical method for determining the age of unknown individuals. However, epigenetic clocks, a molecular-based method, are capable of age prediction by sampling specific tissue types and measuring DNA methylation levels at specific loci. Developing an epigenetic clock requires a large number of samples from animals of known ages. For most species, there are no individuals whose exact ages are known, making epigenetic clock calibration inaccurate or impossible. For many epigenetic clocks, calibration samples with inaccurate age estimates introduce a degree of error to epigenetic clock calibration. In this study, we investigated how much error in the training data set of an epigenetic clock can be tolerated before it resulted in an unacceptable increase in error for age prediction. Using four publicly available data sets, we artificially increased the training data age error by iterations of 1% and then tested the model against an independent set of known ages. A small effect size increase (Cohen's d &gt;0.2) was detected when the error in age was higher than 22%. The effect size increased linearly with age error. This threshold was independent of sample size. Downstream applications for age data may have a more important role in deciding how much error can be tolerated for age prediction. If highly precise age estimates are required, then it may be futile to embark on the development of an epigenetic clock when there is no accurately aged calibration population to work with. However, for other problems, such as determining the relative age order of pairs of individuals, a lower-quality calibration data set may be adequate.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1496-1502"},"PeriodicalIF":4.1,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13582","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5834528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative life-history strategy contributions to effective population size in a naturally spawning salmon population","authors":"Erika King,&nbsp;Megan V. McPhee,&nbsp;Scott C. Vulstek,&nbsp;Curry J. Cunningham,&nbsp;Joshua R. Russell,&nbsp;David A. Tallmon","doi":"10.1111/eva.13580","DOIUrl":"https://doi.org/10.1111/eva.13580","url":null,"abstract":"<p>Alternative life-history tactics are predicted to affect within-population genetic processes but have received little attention. For example, the impact of precocious males on effective population size (<i>N</i>_e) has not been quantified directly in Pacific salmon <i>Oncorhynchus</i> spp., even though they can make up a large percentage of the total male spawners. We investigated the contribution of precocial males (“jacks”) to <i>N</i>_e in a naturally spawning population of Coho Salmon <i>O. kisutch</i> from the Auke Creek watershed in Juneau, Alaska. Mature adults that returned from 2009 to 2019 (~8000 individuals) were genotyped at 259 single-nucleotide polymorphism (SNP) loci for parentage analysis. We used demographic and genetic methods to estimate the effective number of breeders per year (<i>N</i>_b). Jack contribution to <i>N</i>_b was assessed by comparing values of <i>N</i>_b calculated with and without jacks and their offspring. Over a range of <i>N</i>_b values (108–406), the average jack contribution to <i>N</i>_b from 2009 to 2015 was 12.9% (SE = 3.8%). Jacks consistently made up over 20% of the total male spawners. The presence of jacks did not seem to influence <i>N</i>_b/<i>N</i>. The linkage disequilibrium <i>N</i>_e estimate was lower than the demographic estimate, possibly due to immigration effects on population genetic processes: based on external marks and parentage data, we estimated that immigrant spawners produced 4.5% of all returning offspring. Our results demonstrate that jacks can influence <i>N</i>_b and <i>N</i>_e and can make a substantial contribution to population dynamics and conservation of threatened stocks.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1472-1482"},"PeriodicalIF":4.1,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13580","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5652681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can cryptic female choice prevent invasive hybridization in external fertilizing fish?","authors":"Tyler H. Lantiegne,&nbsp;Craig F. Purchase","doi":"10.1111/eva.13573","DOIUrl":"https://doi.org/10.1111/eva.13573","url":null,"abstract":"<p>Polyandrous mating systems result in females mating with multiple males, generating opportunities for strong pre-mating and post-mating sexual selection. Polyandry also creates the potential for unintended matings and subsequent sperm competition with hybridizing species. Cryptic female choice allows females to bias paternity towards preferred males under sperm competition and may include conspecific sperm preference when under hybridization risk. The potential for hybridization becomes particularly important in context of invasive species that can novelly hybridize with natives, and by definition, have evolved allopatrically. We provide the first examination of conspecific sperm preference in a system of three species with the potential to hybridize: North American native Atlantic salmon (<i>Salmo salar</i>) and brook char (<i>Salvelinus fontinalis</i>), and invasive brown trout (<i>Salmo trutta</i>) from Europe. Using naturalized populations on the island of Newfoundland, we measured changes in sperm swimming performance, a known predictor of paternity, to determine the degree of modification in sperm swimming to female cues related to conspecific sperm preference. Compared to water alone, female ovarian fluid in general had a pronounced effect and changed sperm motility (by a mean of 53%) and swimming velocity (mean 30%), but not linearity (mean 6%). However, patterns in the degree of modification suggest there is no conspecific sperm preference in the North American populations. Furthermore, female cues from both native species tended to boost the sperm of invasive males more than their own. We conclude that cryptic female choice via ovarian fluid mediated sperm swimming modification is too weak in this system to prevent invasive hybridization and is likely insufficient to promote or maintain reproductive isolation between the native North American species.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1412-1421"},"PeriodicalIF":4.1,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13573","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5672827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-specific heritabilities for length at maturity among Pacific salmonids and their consequences for evolution in response to artificial selection","authors":"Madilyn M. Gamble,&nbsp;Ryan G. Calsbeek","doi":"10.1111/eva.13579","DOIUrl":"https://doi.org/10.1111/eva.13579","url":null,"abstract":"<p>Artificial selection, whether intentional or coincidental, is a common result of conservation policies and natural resource management. To reduce unintended consequences of artificial selection, conservation practitioners must understand both artificial selection gradients on traits of interest and how those traits are correlated with others that may affect population growth and resilience. We investigate how artificial selection on male body size in Pacific salmon (<i>Oncorhynchus</i> spp.) may influence the evolution of female body size and female fitness. While salmon hatchery managers often assume that selection for large males will also produce large females, this may not be the case—in fact, because the fastest-growing males mature earliest and at the smallest size, and because female age at maturity varies little, small males may produce larger females if the genetic architecture of growth rate is the same in both sexes. We explored this possibility by estimating sex-specific heritability values of and natural and artificial selection gradients on length at maturity in four populations representing three species of Pacific salmon. We then used the multivariate breeder's equation to project how artificial selection against small males may affect the evolution of female length and fecundity. Our results indicate that the heritability of length at maturity is greater within than between the sexes and that sire–daughter heritability values are especially small. Salmon hatchery policies should consider these sex-specific quantitative genetic parameters to avoid potential unintended consequences of artificial selection.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1458-1471"},"PeriodicalIF":4.1,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6204143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-scale population structure of the northern hard clam (Mercenaria mercenaria) revealed by genome-wide SNP markers","authors":"Ann J. Ropp,&nbsp;Kimberly S. Reece,&nbsp;Richard A. Snyder,&nbsp;Jingwei Song,&nbsp;Ellen E. Biesack,&nbsp;Jan R. McDowell","doi":"10.1111/eva.13577","DOIUrl":"https://doi.org/10.1111/eva.13577","url":null,"abstract":"<p>Aquaculture is growing rapidly worldwide, and sustainability is dependent on an understanding of current genetic variation and levels of connectivity among populations. Genetic data are essential to mitigate the genetic and ecological impacts of aquaculture on wild populations and guard against unintended human-induced loss of intraspecific diversity in aquacultured lines. Impacts of disregarding genetics can include loss of diversity within and between populations and disruption of local adaptation patterns, which can lead to a decrease in fitness. The northern hard clam, <i>Mercenaria mercenaria</i> (Linnaeus, 1758), is an economically valuable aquaculture species along the North American Atlantic and Gulf coasts. Hard clams have a pelagic larval phase that allows for dispersal, but the level of genetic connectivity among geographic areas is not well understood. To better inform the establishment of site-appropriate aquaculture brood stocks, this study used DArTseq™ genotyping by sequencing to characterize the genetic stock structure of wild clams sampled along the east coast of North America and document genetic diversity within populations. Samples were collected from 15 locations from Prince Edward Island, Canada, to South Carolina, USA. Stringent data filtering resulted in 4960 single nucleotide polymorphisms from 448 individuals. Five genetic breaks separating six genetically distinct populations were identified: Canada, Maine, Massachusetts, Mid-Atlantic, Chesapeake Bay, and the Carolinas (<i>F</i>_ST 0.003–0.046; <i>p</i> &lt; 0.0001). This is the first study to assess population genetic structure of this economically important hard clam along a large portion of its native range with high-resolution genomic markers, enabling identification of previously unrecognized population structure. Results of this study not only broaden insight into the factors shaping the current distribution of <i>M. mercenaria</i> but also reveal the genetic population dynamics of a species with a long pelagic larval dispersal period along the North American Atlantic and Gulf coasts.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"16 8","pages":"1422-1437"},"PeriodicalIF":4.1,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.13577","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5794165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}