Evolutionary Applications最新文献

{"title":"Inbreeding and Demographic History of Caribou (Rangifer tarandus) in Western Canada Inferred From Genome-Wide SNP Data","authors":"Charlotte Bourbon,&nbsp;Samuel Deakin,&nbsp;Anita Michalak,&nbsp;Margaret M. Hughes,&nbsp;Maria Cavedon,&nbsp;Lalenia Neufeld,&nbsp;Agnès Pelletier,&nbsp;Jean Polfus,&nbsp;Helen Schwantje,&nbsp;Caeley Thacker,&nbsp;Marco Musiani,&nbsp;Jocelyn Poissant","doi":"10.1111/eva.70311","DOIUrl":"https://doi.org/10.1111/eva.70311","url":null,"abstract":"<p>Assessing genetic diversity is essential for conserving endangered populations, yet comprehensive genomic evaluations remain limited for many declining species. Here, we investigated inbreeding levels and effective population sizes (N_e) of caribou (<i>Rangifer tarandus</i>) in western Canada, where populations have experienced pronounced declines over the past centuries due to anthropogenic pressures and climate change. We analyzed 33,346 Single Nucleotide Polymorphisms (SNPs) from 759 individuals representing 45 subpopulations within six metapopulations to: (1) assess inbreeding using runs of homozygosity (ROHs), (2) estimate contemporary and historical N_e at the subpopulation, genetic unit, and metapopulation levels, and (3) evaluate relationships between census size (N_c), inbreeding, and N_e. Small and endangered subpopulations, predominantly in southern regions, generally exhibited high inbreeding (F_ROH &gt; 0.1), although some larger populations also showed elevated levels. Most subpopulations displayed a mixture of short and long ROHs, indicating both ancient shared ancestry and recent inbreeding. Twelve subpopulations had N_e &lt; 50, and 28 subpopulations had N_e &lt; 500, and three of six metapopulations fell below the N_e &lt; 500 threshold once population structure was accounted for, suggesting compromised short-term viability and long-term adaptive potential in the most affected units. N_c significantly predicted inbreeding (<i>R</i>² = 0.16), whereas contemporary N_e did not. Historical N_e reconstructions revealed a north-to-south gradient in bottleneck timing: northern populations declined in ~1700–1780, central populations in ~1780–1860, and southern populations in ~1860–1940, broadly coinciding with the documented timing of climate shifts and anthropogenic disturbances in the region. Our findings identify at-risk populations requiring urgent genetic intervention and demonstrate that integrating inbreeding and N_e estimates provides a robust framework for caribou recovery and the management of fragmented wildlife populations.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Landscape Context and Environmental Stochasticity Shape Genetic Management Outcomes in an Amphibian","authors":"Hanne Haugen,&nbsp;Børre K. Dervo,&nbsp;Arne Linløkken,&nbsp;Jan Heggenes,&nbsp;Kjartan Østbye","doi":"10.1111/eva.70310","DOIUrl":"10.1111/eva.70310","url":null,"abstract":"<p>Landscape context can influence demographic rates and, consequently, the population genetic processes determining levels of genetic diversity. However, the role of landscape context is not always sufficiently considered in conservation management. For species with complex life cycles, such as amphibians, landscape context is particularly relevant because they rely on both aquatic and terrestrial habitats, the quality of which can vary across landscapes. Understanding how landscape context may influence these genetic processes is therefore important for effective management. We used population viability analysis (PVA) to simulate how landscape context may influence genetic and demographic outcomes for the great crested newt (<i>Triturus cristatus</i>) using two landscape-specific models representing boreal forest and agricultural landscapes. We assessed the sensitivity of model outcomes to parameter perturbations and evaluated management scenarios and potential threats at the metapopulation level. A key result was that environmental stochasticity strongly influenced genetic dynamics in both landscapes. The low-stochasticity forest model retained genetic diversity, responded strongly to management actions, and relied less on gene flow. In contrast, the high-stochasticity agricultural model experienced stronger genetic drift, responded weakly to management actions, and relied more on continuous gene flow. Our findings suggest that enhancing gene flow alone may be insufficient for maintaining genetic diversity, and that improving resilience to environmental stochasticity should be considered an important part of genetic management.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Within-Island Diversification Generates Lineage-Specific Climate Vulnerability: Insights From the Taiwan-Endemic Formosan Duke","authors":"Zong-Yu Shen,&nbsp;Yu-Feng Hsu,&nbsp;Masaya Yago,&nbsp;Chih-Chien Lu,&nbsp;Ming-Yu Chen,&nbsp;Wei-Yun Chen,&nbsp;Tzong-Han Lin,&nbsp;Jen-Pan Huang,&nbsp;Li-Wei Wu","doi":"10.1111/eva.70309","DOIUrl":"10.1111/eva.70309","url":null,"abstract":"<p>Global climate change disproportionately threatens island endemics because their restricted ranges and limited dispersal opportunities constrain their ability to shift their distributions. Therefore, when developing conservation strategies for species, it is crucial to incorporate population genomics data with ecological information to evaluate resilience and forecast vulnerability. However, genomic case studies that explicitly link these factors in island endemics remain scarce. Here, we examine population genetic differentiation of the Formosan Duke (<i>Euthalia formosana</i>), an endemic butterfly in Taiwan, to evaluate its potential vulnerabilities under future climate conditions. Our results reveal that the combined effects of topographic barriers, historical connectivity, and climatic heterogeneity can drive rapid diversification, even in species with strong flight ability. The Central Mountain Range has shaped pronounced genetic structure, separating eastern and western lineages, with diversification stronger in the western lineage. This within-island diversification appears to be shaped by environmental gradients, enhancing persistence in local habitats while reducing a lineage's niche breadth and resulting in different lineage-specific climate vulnerability. Thus, we suggest that genomic approaches should be included to assess environmental constraints and to guide conservation planning on the persistence of lowland endemics such as the Formosan Duke. This framework also integrates the concepts of habitat restoration with lineage-specific connectivity to enhance the species' genetic resilience while preserving its capacity for local adaptation. Moreover, this study provides an evidence-based framework for insular biodiversity conservation under accelerating climate change.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13430063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungicide Drives De Novo Evolution of Multidrug Resistance in the Plant Growth Promoting Rhizobacterium, Pseudomonas fluorescens","authors":"Matthew Kelbrick,&nbsp;James P. J. Hall,&nbsp;Siobhán O'Brien","doi":"10.1111/eva.70307","DOIUrl":"10.1111/eva.70307","url":null,"abstract":"<p>Soil microbial communities underpin key ecosystem processes, including plant health, nutrient cycling and primary productivity. In agricultural soils, beneficial soil bacteria are often exposed to multiple stressors simultaneously, including fungicides, antibiotics, and warming temperatures. Despite their ecological importance, little is known about how beneficial soil bacteria respond to such combined stressors over evolutionary timescales. Here, we investigated how the model plant growth promoting rhizobacterium (PGPR) <i>Pseudomonas fluorescens</i> evolves resistance to the fungicide formulation Fubol Gold (metalaxyl-M + mancozeb), under ambient or warming soil conditions. Using a 16-week evolution experiment in soil microcosms with a fully factorial design (fungicide ± warming), we assessed the evolution of fungicide resistance via phenotypic assays and whole-genome sequencing. Fungicide exposure rapidly selected for increased fungicide resistance, detectable as early as week 4, and co-selected for resistance to chloramphenicol, sulphatriad and nalidixic acid antibiotics, likely through mutations in a <i>mexS</i> ortholog that may be associated with efflux pump overexpression. Warming did not alter the evolution of fungicide resistance; however, populations subjected to both fungicide and warming stress went extinct more rapidly, so that population evolutionary rescue was less effective under dual stress. Our findings show that fungicide alone can drive multidrug resistance in beneficial soil bacteria, suggesting that non-antibiotic drivers of resistance in the environment should be incorporated into One Health frameworks for tackling AMR.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13430274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions Between Inbreeding, Fitness and the Bacterial Microbiome in Aedes aegypti Mosquitoes","authors":"Perran A. Ross,&nbsp;Nadieh de Jonge,&nbsp;Qiong Yang,&nbsp;Véronique Paris,&nbsp;Torsten N. Kristensen,&nbsp;Ary A. Hoffmann","doi":"10.1111/eva.70308","DOIUrl":"https://doi.org/10.1111/eva.70308","url":null,"abstract":"<p>Laboratory and field populations of insects can experience a decline in fitness and loss of genetic diversity due to inbreeding depression and genetic drift, respectively. Matings among related individuals and small population size may also influence insect host microbiomes with consequences for fitness. In the dengue vector mosquito, <i>Aedes aegypti</i>, the bacterial microbiome is largely environmentally determined, but recent studies have also revealed host genetic components. We generated a panel of 55 inbred lines from either of two founding outbred populations of <i>Ae. aegypti</i> to test for associations between life history traits, inbreeding, allelic diversity, and microbiome composition using ddRADseq and bacterial 16S rRNA gene sequencing on pools of mosquitoes. Effects of inbreeding were diverse, with severe composite fitness costs in many lines but minimal costs in others despite similar low levels of genetic diversity. We found no strong relationship between major life history traits across inbred lines, suggesting that any costs due to inbreeding were trait specific. Bacterial microbiome analysis of pooled samples from a subset of lines revealed common microbes across lines, with <i>Elizabethkingia</i>, <i>Aeromonas</i>, and <i>Ralstonia</i> being the most abundant. Despite bacterial composition varying widely, there was no clear relationship between microbiome composition and fitness or population origin. However, there were several significant positive correlations between the relative abundance of different microbial taxa across lines. Our results demonstrate diverse impacts of inbreeding on the fitness of mosquito populations but with limited impacts on the microbiome.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70308","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraspecific Hybridization Improves Performance of the Biological Control Agent Hypena opulenta","authors":"Brianna Foster,&nbsp;Ruth. A. Hufbauer,&nbsp;Marianna Szűcs","doi":"10.1111/eva.70306","DOIUrl":"https://doi.org/10.1111/eva.70306","url":null,"abstract":"<p>Introduced species often undergo genetic drift and natural selection, which can reduce genetic diversity and drive differentiation among populations. Intraspecific hybridization, outcrossing among individuals from genetically distinct groups, may counteract these effects by increasing heterozygosity, potentially improving performance. However, in classical biological control agents, genetic diversity is typically low due to small founding sizes, long-term quarantine rearing, and single-source collections from the native range. As a result, opportunities for beneficial hybridization may be limited. We evaluated whether intraspecific hybridization could enhance genetic diversity and performance of <i>Hypena opulenta</i> (Lepidoptera: Erebidae), a biological control agent of invasive swallow-wort vines (<i>Vincetoxicum</i> spp.) in North America. We used two populations: one reared under laboratory conditions for 28 generations, and another established in the field in Canada, separated from the lab population for 14 generations. We created a third population by crossing individuals from the two source populations. We conducted Whole Genome Sequencing (WGS) on 15–20 individuals per population to assess genetic divergence and heterozygosity and compared performance of the three populations under both laboratory and field conditions. Despite relatively recent separation, clear genetic structure had developed between the lab and field populations. Both exhibited low genome-wide heterozygosity, while the outbred population showed significantly higher heterozygosity. Hybridization also led to improved performance: outbred females produced more pupae in the laboratory, caused greater defoliation of host plants, and yielded the most second-generation adults in the field. These results demonstrate that even short-term isolation can lead to meaningful divergence and that intentional intraspecific hybridization can boost both genetic diversity and performance. Our findings suggest that outcrossing among recently separated populations could be a practical approach to improve performance of biological control agents prior to field release, with broader implications for managing genetic diversity in applied ecology and conservation contexts.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Genomic Information for Identifying the Geographic Origin of Five North American Tree Species in Trade","authors":"Pauline Hessenauer,&nbsp;Melanie Zacharias,&nbsp;Julien Prunier,&nbsp;Anna Fijarczyk,&nbsp;Roos Goessen,&nbsp;Julie Godbout,&nbsp;Anthony Piot,&nbsp;Isabelle Duchesne,&nbsp;Sam Yeaman,&nbsp;Ilga Porth,&nbsp;Nathalie Isabel","doi":"10.1111/eva.70295","DOIUrl":"https://doi.org/10.1111/eva.70295","url":null,"abstract":"<p>Genomic tools for traceability of wood products offer a powerful tool to support sustainable forestry, fight illegal logging, and improve conservation efforts. Traditional identification methods (e.g., wood anatomy, spectroscopy) are limited in resolution or scope, but genomic approaches can infer both species identity and geographic origin. Using lodgepole pine (<i>Pinus contorta</i>) as a case study, we first compared three types of SNP datasets—random, adaptation-linked, and machine learning-selected—for their effectiveness to assign origin. While group-based assignment methods (e.g., geographic or genetic) performed well with a small number of groups, their accuracy declined sharply as the number of groups increased, though it remained above random expectations even with 281 groups. To address this limitation, we developed a coordinate-based methodological framework that directly predicts geographic origin from SNP marker sets of varying sizes. We applied this framework to four additional North American tree species (<i>Populus trichocarpa</i>, black cottonwood; <i>P. tremuloides</i>, quaking aspen; <i>Picea mariana</i>, black spruce; <i>Pinus strobus</i>, eastern white pine), which represent a range of evolutionary histories and population structures. Using both classical and machine learning algorithms, including Linear Model (LM), K-Nearest Neighbor (KNN), Random Forest (RF), and Gradient Boosting (GB), we predicted latitude and longitude with mean distance errors ranging from 15 to 383 km, depending on the species. Accuracy varied depending on species-specific attributes such as population structure and sampling density. KNN performed best in datasets with high sampling density, such as <i>P. trichocarpa</i>, while GB achieved superior performance in more genetically homogenous taxa like <i>P. strobus</i>. This flexible, data-driven approach enables precise traceability across tree species and supports forensic, regulatory, and certification uses. We outline practical guidelines emphasizing accurate taxonomic identification, broad sampling, and the use of ~2000–5000 random genomic markers. Although KNN and Gradient Boosting generally performed well, species-specific differences warrant testing multiple algorithms.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Implications of Plant Sourcing Strategies for Salt Marsh Restoration","authors":"A. Randall Hughes,&nbsp;Serena Caplins,&nbsp;Neelima Cherukuri,&nbsp;Madeleine DuChaussee,&nbsp;Gabriela Garcia,&nbsp;Peter Garneau,&nbsp;Tessa McClain,&nbsp;Andrew McQueen,&nbsp;Katherine Sperry,&nbsp;Sarit Truskey,&nbsp;Erik E. Sotka","doi":"10.1111/eva.70292","DOIUrl":"https://doi.org/10.1111/eva.70292","url":null,"abstract":"<p>Ecosystem restoration aims to recreate the ecological structure, processes, and function of a reference native ecosystem. The structure and composition of restored ecosystems often differ from natural ecosystems, at least in part due to human decisions about which ecosystem characteristics and functions to prioritize. We generally lack an understanding of the precise steps in the restoration process that contribute to those differences, but decisions regarding how and where to source biological material are likely to play a role. Here, we use salt marshes as a system to test the potential for sourcing decisions to influence the genetic diversity and composition of two dominant plant species, <i>Spartina alterniflora</i> and <i>S. patens</i>, in restored salt marshes. We show that there is weak but detectable genetic differentiation across local populations that could serve as sources for restoration efforts, though the magnitude of this differentiation varied by analysis and across the two plant species. In addition, the degree of differentiation is generally greater among plant stocks produced from seed from these potential source populations than among the original populations themselves. Most clearly, plants from commercial nursery sources generally have higher genetic diversity and were highly differentiated from both the local populations and seedlings produced from seed from those local populations. These results are consistent with recent studies showing that restored marshes have distinct genetic characteristics from adjacent natural marshes. Given the demonstrated links between plant genetic variation and ecosystem function and resilience, differences in genetic variation resulting from sourcing decisions have the potential to impact the success of restoration efforts and should be an explicit component of restoration practice.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70292","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Insights Into Population Structure and Demographic History of White Anglerfish (Lophius piscatorius) Throughout Its Range","authors":"Atal Saha,&nbsp;María Quintela,&nbsp;Kjell Nedreaas,&nbsp;Naiara Rodríguez-Ezpeleta,&nbsp;François Besnier,&nbsp;Hans Gerritsen,&nbsp;Halvor Knutsen,&nbsp;Rita Castilho,&nbsp;Karen Agatha Martínez-Swatson,&nbsp;Kevin Glover,&nbsp;Per Erik Jorde","doi":"10.1111/eva.70288","DOIUrl":"10.1111/eva.70288","url":null,"abstract":"<p>Understanding population structure and clarifying species boundaries are essential for the sustainable management of exploited marine species. Although genomic resources for shallow-water taxa have expanded rapidly, open-water species remain comparatively understudied. Here, we present a comprehensive genomic analysis of the white anglerfish (<i>Lophius piscatorius</i>), a commercially important species that occupies a wide depth range. We examined genetic structuring and verified species identity across its distribution range in the North Atlantic and Mediterranean. We combined whole-genome sequencing of selected individuals with targeted SNP genotyping of 897 specimens from 35 locations. Our results support a panmictic or nearly panmictic population of white anglerfish throughout the Northeast Atlantic, consistent with earlier studies, here extended northward to about 68° N. We found no evidence of local adaptation or selection. Like earlier studies, we detected apparently misclassified specimens of the closely related black anglerfish (<i>L. budegassa</i>) and extensive hybridization with up to 20% hybrids in local samples from around the Celtic Sea and nearby waters. Most or all hybrids appear to be first generation (F1), with a few potential backcrossed individuals of less certain identification. Demographic analyses indicate a recent colonization of the North Atlantic, with black anglerfish diverging more recently and maintaining a smaller effective population size. These results underscore the value of integrating genomic tools into monitoring frameworks to improve species identification, delineate population units, and inform sustainable fisheries management.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148589765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gametophyte Thermal Priming Modifies the Transcriptomic Heat Stress Response of Sporophyte Progeny in Saccharina latissima","authors":"Anne M. L. Nilsen,&nbsp;Niko Steiner,&nbsp;Inka Bartsch,&nbsp;J. Mark Cock,&nbsp;Alexander O. Jueterbock","doi":"10.1111/eva.70301","DOIUrl":"10.1111/eva.70301","url":null,"abstract":"<p><i>Saccharina latissima</i> (sugar kelp) is a brown macroalga that forms kelp forests along North Atlantic coasts, playing a key role in coastal ecosystems. It is also of growing economic importance as the dominant species used in European kelp aquaculture. Both wild and cultivated kelps are increasingly threatened by marine heatwaves and elevated sea surface temperatures. Enhancing thermal tolerance could therefore benefit both conservation and production. Thermal priming, a method originating in agriculture where early life stages are exposed to moderate heat stress, can enhance resilience by inducing molecular stress memory. We applied a thermal priming treatment (20°C, 3 weeks) to <i>S. latissima</i> gametophytes before gametogenesis and sporophyte formation to test whether priming alters thermal responses in the derived sporophytes. Young sporophytes, produced by crossing primed or naïve (controls kept at 10°C) gametophytes, were reared at 10°C and then exposed to a heat stress (21.5°C for 48 h) before being allowed to recover at 10°C. Transcriptomic profiles and photophysiological characteristics were assessed before, during and after stress exposure to investigate priming-induced changes in thermal stress resilience. Sporophytes derived from primed gametophytes showed stronger transcriptomic responses under heat stress; for example, upregulation of HSP90B was unique to primed samples. Naïve sporophytes exhibited a delayed response with extensive downregulation during recovery (2027 DEGs). Gene ontology enrichment analysis indicated higher expression of protein phosphorylation, chloroplast-related functions, and post-transcriptional regulation by ncRNA in primed sporophytes, suggesting a priming effect on their transcriptomic thermal response. Primed sporophytes exhibited a delayed reduction in photosynthetic performance compared with naïve throughout the heat stress. These findings suggest that thermal priming induces a transgenerational stress memory. Our results support the view that temperature priming alters the transcriptomic thermal response in kelp through regulatory changes in gene expression, in line with existing literature on priming.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13395404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}