Acta Naturae最新文献

Combination with a Low Dose of Doxorubicin Further Boosts the Antitumor Effect of SLURP-1 In Vivo and Associates with EGFR Down-Regulation. 低剂量阿霉素联用进一步增强SLURP-1体内抗肿瘤作用并与EGFR下调相关。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27526

O V Shlepova, M L Bychkov, V O Shipunova, E I Shramova, M A Shulepko, T Y Gornostaeva, E A Kiseleva, I D Kukushkin, V A Kazakov, E A Tukhovskaya, I A Dyachenko, A N Murashev, Z O Shenkarev, S M Deyev, M P Kirpichnikov, E N Lyukmanova

{"title":"Combination with a Low Dose of Doxorubicin Further Boosts the Antitumor Effect of SLURP-1 In Vivo and Associates with EGFR Down-Regulation.","authors":"O V Shlepova, M L Bychkov, V O Shipunova, E I Shramova, M A Shulepko, T Y Gornostaeva, E A Kiseleva, I D Kukushkin, V A Kazakov, E A Tukhovskaya, I A Dyachenko, A N Murashev, Z O Shenkarev, S M Deyev, M P Kirpichnikov, E N Lyukmanova","doi":"10.32607/actanaturae.27526","DOIUrl":"https://doi.org/10.32607/actanaturae.27526","url":null,"abstract":"Skin cancers such as squamous cell carcinoma (SCC) are among the most aggressive types of tumors. They come with a high rate of growth, metastasis, and frequently occurring chemoresistance. Smoking is one of the risk factors for SCC progression, and the α7 nicotinic acetylcholine receptor (α7-nAChR) is a promising target for SCC therapy. Human secreted protein SLURP-1 is an auto/paracrine regulator of epithelial homeostasis and a selective negative allosteric modulator of α7-nAChR. Recently, we demonstrated the high efficiency of the therapy based on the recombinant SLURP-1 in controlling SCC cell growth and metastasis in vivo. The anti-tumor effect of SLURP-1 was mediated through interaction with both α7-nAChR and the epidermal growth factor receptor (EGFR). Cytotoxic antibiotic doxorubicin has been proposed for the SCC therapy; however, its use is limited due to the high toxicity. In this study we investigated the use of an enhanced SLURP-1 dose and of a combination of SLURP-1 with low-dozen doxorubicin for SCC treatment of mice xenografted with squamous cell carcinoma A431 cells. An increased SLURP-1 dose didn't significantly enhance the efficiency of the therapy. However, the combination with doxorubicin further enhanced the anti- tumor activity of SLURP-1 and dramatically suppressed metastasis. The effect from the combined therapy was accompanied by down-regulation of EGFR expression in tumors. Direct inhibition of EGFR activation by SLURP-1 was shown. No toxicity of the combined therapy was encountered. Our data indicate that the combination of SLURP-1 with chemotherapy in lower doses is a promising approach in SCC treatment and should be further studied.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"87-96"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations in the Expression of Proprotein Convertase Genes in Human Esophagus Squamous Cell Carcinomas. 人食管鳞状细胞癌中蛋白转化酶基因表达的改变。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27437

A A Komissarov, M V Zinovyeva, A V Sass, T V Vinogradova, S I Koshechkin, V V Demkin, I B Zborovskaya, S V Kostrov, I V Demidyuk

{"title":"Alterations in the Expression of Proprotein Convertase Genes in Human Esophagus Squamous Cell Carcinomas.","authors":"A A Komissarov, M V Zinovyeva, A V Sass, T V Vinogradova, S I Koshechkin, V V Demkin, I B Zborovskaya, S V Kostrov, I V Demidyuk","doi":"10.32607/actanaturae.27437","DOIUrl":"https://doi.org/10.32607/actanaturae.27437","url":null,"abstract":"Proprotein convertases (PCs) constitute an enzyme family that includes nine highly specific human subtilisin-like serine proteases. It is known that the PCs mRNA levels vary in tumors, and that these proteases are involved in carcinogenesis. Thus, PCs may be considered as potential markers for typing and predicting the course of the disease, as well as potential targets for therapy. We used quantitative real-time PCR to evaluate the expression levels of PC genes in the paired samples of tumor and adjacent normal tissues derived from 19 patients with esophageal squamous cell carcinomas. We observed a significant enrichment of PCSK6, PCSK9, MBTPS1, and FURIN mRNAs in the tumor tissue, which may be indication of the involvement of these PCs in the development and progression of esophageal cancers. Additionally, cluster analysis of PC expression alteration patterns in tumor compared to normal adjacent tissues (esophageal and previously analyzed lung tissue samples) revealed a limited set of scenarios for the changes in PC expression. These scenarios are implemented during malignant transformation of lung and esophagus cells, as well as, probably, the cells of other organs. These findings indicate that PC genes may be important markers of human cancers.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"64-70"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of Chaperone Synthesis in Human Neuronal Cells Blocks Oxidative Stress-Induced Aging. 诱导神经细胞伴侣蛋白合成阻断氧化应激诱导的衰老。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27531

E A Dutysheva, L S Kuznetcova, I A Utepova, B A Margulis, I V Guzhova, V F Lazarev

引用次数: 0

The Toxin-Producing Ability of Fusarium Proliferatum Strains Isolated from Grain. 粮食中增殖镰刀菌产毒能力的研究。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27546

O P Gavrilova, A S Orina, T Yu Gagkaeva, N N Gogina

{"title":"The Toxin-Producing Ability of Fusarium Proliferatum Strains Isolated from Grain.","authors":"O P Gavrilova, A S Orina, T Yu Gagkaeva, N N Gogina","doi":"10.32607/actanaturae.27546","DOIUrl":"https://doi.org/10.32607/actanaturae.27546","url":null,"abstract":"The widespread fungus Fusarium proliferatum can infect numerous plant species and produce a range of mycotoxins, the amount of which can vary significantly. Twelve F. proliferatum sensu lato strains isolated from six wheat, four oat, and two maize grain samples were analyzed. The strains were identified through a phylogenetic analysis of nucleotide sequences derived from gene fragments of the translation elongation factor EF-1α, β-tubulin, and RNA polymerase II second subunit. The mating types of the strain were determined by allele-specific PCR. Secondary toxic metabolite production by the strains was quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). All twelve Fusarium strains formed a distinct clade alongside the F. proliferatum reference strains, thereby confirming the taxonomic identification. Only one idiomorph at the MAT locus in each F. proliferatum strain was found, indicative of heterothallic mating. The frequency of the MAT1-1 idiomorph was double that of the MAT1-2 idiomorph. The active biosynthesis of fumonisins B1 (71-6175 mg/kg), B2 (12-2661 mg/kg), and B3 (6-588 mg/kg), significant beauvericin (64-455 mg/kg), and trace amounts of moniliformin (12-6565 μg/kg) were identified across all examined F. proliferatum strains.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"20-28"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galanin Reduces Myocardial Ischemia/Reperfusion Injury in Rats with Streptozotocin Diabetes. 甘丙肽减轻链脲佐菌素糖尿病大鼠心肌缺血/再灌注损伤。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27506

I M Studneva, L I Serebryakova, O M Veselova, I V Dobrokhotov, M E Palkeeva, D V Avdeev, A S Molokoedov, M V Sidorova, O I Pisarenko

{"title":"Galanin Reduces Myocardial Ischemia/Reperfusion Injury in Rats with Streptozotocin Diabetes.","authors":"I M Studneva, L I Serebryakova, O M Veselova, I V Dobrokhotov, M E Palkeeva, D V Avdeev, A S Molokoedov, M V Sidorova, O I Pisarenko","doi":"10.32607/actanaturae.27506","DOIUrl":"https://doi.org/10.32607/actanaturae.27506","url":null,"abstract":"Most clinical studies confirm the negative impact diabetes mellitus (DM) has on the course and outcome of cardiovascular complications caused by a myocardial ischemia-reperfusion injury (IRI). In this regard, the search for new approaches to IRI treatment in diabetic myocardium is of undeniable value. The aim of this work was to study the effect of galanin (G) on the size of myocardial infarct (MI), on mitochondrial functions, and on the energy state in the area at risk (AAR) in rats with type 1 diabetes mellitus (DM1) subjected to regional myocardial ischemia and reperfusion. Rat G was obtained by solid-phase synthesis using the Fmoc strategy and purified by HPLC. DM1 was induced by streptozotocin administration. Myocardial IRI was modeled by occlusion of the left anterior descending coronary artery and subsequent reperfusion. G at a dose of 1 mg/kg was administered intravenously before reperfusion. G decreased MI size and plasma creatine kinase MB (CK-MB) activity in DM rats by 40 and 28%, respectively. G injection improved mitochondrial respiration in saponin-skinned fibers in the AAR: namely, the maximal ADP-stimulated state 3, respiratory control, and the functional relationship between the mitochondrial CK-MB and oxidative phosphorylation. G provided significantly higher ATP levels, total adenine nucleotide pool, and adenylate energy charge of cardiomyocytes. It also reduced total creatine loss in myocardial AAR in DM rats. The results suggest there is a possibility of therapeutic use of G in myocardial IRI complicated by DM1.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"78-86"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pro-inflammatory Cytokines, Ferroptosis, and Cancer. 促炎细胞因子，铁下垂和癌症。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27547

A A Vartanian, V S Kosorukov

引用次数: 0

The Parameters of Long-Term Humoral Immunity Induced by a Single Injection of the Sputnik Light Vaccine Among Noninfected Volunteers and Those Infected with SARS-CoV-2. 非感染志愿者和SARS-CoV-2感染者单次注射Sputnik轻型疫苗长期体液免疫参数的研究

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27529

F M Izhaeva, A I Tukhvatulin, A S Dzharullaeva, I V Dolzhikova, O V Zubkova, D V Shcheblyakov, N L Lubenets, E A Smolyarchuk, T G Zubkova, A S Erokhova, I M Cherenkova, K M Bukhtin, O Popova, N M Tukhvatulina, B S Naroditsky, D Y Logunov, A L Gintsburg

{"title":"The Parameters of Long-Term Humoral Immunity Induced by a Single Injection of the Sputnik Light Vaccine Among Noninfected Volunteers and Those Infected with SARS-CoV-2.","authors":"F M Izhaeva, A I Tukhvatulin, A S Dzharullaeva, I V Dolzhikova, O V Zubkova, D V Shcheblyakov, N L Lubenets, E A Smolyarchuk, T G Zubkova, A S Erokhova, I M Cherenkova, K M Bukhtin, O Popova, N M Tukhvatulina, B S Naroditsky, D Y Logunov, A L Gintsburg","doi":"10.32607/actanaturae.27529","DOIUrl":"https://doi.org/10.32607/actanaturae.27529","url":null,"abstract":"Although the immunogenicity of clinically approved COVID-19 vaccines remains under intensive investigation, little is still known about the parameters of long-term immune responses. In this paper, we present for the first time the parameters of humoral immunity studied in the phase 1-2 open-label clinical trial of the Sputnik Light vaccine, with a special focus on late follow-up time points (90 and 180 days). For the most accurate assessment of the parameters of humoral post-vaccination immunity (titer and avidity index of antigen-specific antibodies against the RBD domain of SARS-CoV-2), we conducted an additional analysis that allowed us to triage volunteers with immunity formed only in response to vaccination, as well as those with hybrid immunity (infected with SARS-CoV-2 before and after vaccination). The findings indicate that single-shot vaccination with the Sputnik Light vaccine induces a durable (seroconversion 73% on day 180) and mature humoral immunity. Natural immunization as a result of the SARS-CoV-2 infection leads to significant changes in the studied parameters of post-vaccination immunity.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"52-63"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoregulatory Enzymes. 免疫调节酶。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27549

S V Kupriyanov, K M Kirilenko, D N Starkov

引用次数: 0

Inhibition of HBV Replication by a Fully Humanized Neutralizing Antibody In Vivo and In Vitro. 一种完全人源化中和抗体在体内和体外抑制HBV复制。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27457

Zhipeng Zhang, Yanqin Ma, Yan He, Dong Wang, Huaien Song, Kun Yue, Xiaomei Zhang

{"title":"Inhibition of HBV Replication by a Fully Humanized Neutralizing Antibody In Vivo and In Vitro.","authors":"Zhipeng Zhang, Yanqin Ma, Yan He, Dong Wang, Huaien Song, Kun Yue, Xiaomei Zhang","doi":"10.32607/actanaturae.27457","DOIUrl":"https://doi.org/10.32607/actanaturae.27457","url":null,"abstract":"Neutralizing antibodies are capable of specifically binding to the HBsAg virus, thereby preventing HBV infection and subsequently reducing viral antigen load in both the liver and systemic circulation. This has significant implications for restoring the postnatal immune function. By utilizing the phage antibody library technology, we successfully screened a fully humanized neutralizing antibody targeting the hepatitis B surface antigen. The antiviral activity was assessed in primary human hepatocytes (PHHs) by determining the EC50 values for HBeAg and HBsAg biomarkers in HBV types B, C, and D; no cytotoxicity was observed within the tested concentration range. Furthermore, HT-102 exhibited no ADCC effect but displayed a weak CDC effect along with a dose-dependent response. We established an AAV/HBV mouse model and observed significant dose-dependent reduction in HBsAg and HBV DNA levels for both the medium-dose and highdose groups. The immunohistochemical staining data showed dose-dependent reduction in HBsAg expression in the liver, with high-dose group exhibiting minimal positive expression. Finally, a mild immune response was induced, while reducing the burden of antigen-antibody complexes circulating within the system. Consequently, strain on the patient's immune system was alleviated by effectively slowing down CD8+T lymphocyte depletion, and functional cure was ultimately achieved as intended.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"97-105"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intergeneric Introgression Enhances the Adaptive Potential of Nine-Spined Stickleback (Pungitius pungitius). 属间渗透增强九棘棘鱼（Pungitius Pungitius）的适应性。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27528

A V Nedoluzhko, F S Sharko, S M Rastorguev

引用次数: 0