Acta Naturae最新文献

Insights into the Functioning of the D-amino Acid Transaminase from Haliscomenobacter Hydrossis via a Structural and Spectral Analysis of its Complex with 3-Aminooxypropionic Acid. 通过对 Haliscomenobacter Hydrossis D-amino Acid Transaminase 与 3-Aminooxypropionic Acid 复合物的结构和光谱分析了解其功能。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27496

A K Bakunova, I O Matyuta, A Yu Nikolaeva, K M Boyko, A R Khomutov, E Yu Bezsudnova, V O Popov

{"title":"Insights into the Functioning of the D-amino Acid Transaminase from Haliscomenobacter Hydrossis via a Structural and Spectral Analysis of its Complex with 3-Aminooxypropionic Acid.","authors":"A K Bakunova, I O Matyuta, A Yu Nikolaeva, K M Boyko, A R Khomutov, E Yu Bezsudnova, V O Popov","doi":"10.32607/actanaturae.27496","DOIUrl":"10.32607/actanaturae.27496","url":null,"abstract":"Pyridoxal 5'-phosphate-dependent enzymes play a crucial role in nitrogen metabolism. Carbonyl compounds, such as O-substituted hydroxylamines, stand out among numerous specific inhibitors of these enzymes, including those of practical importance, because they react with pyridoxal 5'-phosphate in the active site of the enzymes to form stable oximes. O-substituted hydroxylamines mimic the side group of amino acid substrates, thus providing highly potent and specific inhibition of the corresponding enzymes. The interaction between D-amino acid transaminase from bacterium Haliscomenobacter hydrossis and 3-aminooxypropionic acid was studied in the present work. The structural and spectral analyses of the complex of this transaminase with 3-aminooxypropionic acid allowed us to clarify some features of the organization and functioning of its active site and illustrate one of the mechanisms of inhibition by the specific substrate, D-glutamic acid.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"18-24"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Correlation Patterns of miRNA Expression with Targeted mRNA Transcripts in Glioma Patients with Wild-Type and Mutated Isocitrate Dehydrogenase (IDH) Genotypes. 野生型和变异型异柠檬酸脱氢酶（IDH）基因型胶质瘤患者体内 miRNA 表达与靶向 mRNA 转录本的相关模式

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27363

A A Bondarev, A S Evpak, A L Novoselov, A A Kudriaeva, A A Jr Belogurov

{"title":"The Correlation Patterns of miRNA Expression with Targeted mRNA Transcripts in Glioma Patients with Wild-Type and Mutated Isocitrate Dehydrogenase (IDH) Genotypes.","authors":"A A Bondarev, A S Evpak, A L Novoselov, A A Kudriaeva, A A Jr Belogurov","doi":"10.32607/actanaturae.27363","DOIUrl":"10.32607/actanaturae.27363","url":null,"abstract":"Low-grade gliomas are divided into two main genetic phenotypes based on the presence or absence of mutations in the isocitrate dehydrogenase (IDH) genes. The mutated IDH phenotype (IDHmut), in contrast to the wild-type phenotype (IDHwt), is characterized by a more positive response to pharmacological intervention and a significantly longer survival time. In this study, we analyzed the differential co-expression of 225,000 microRNA-mRNA pairs at the level of correlations between microRNA levels and their potential mRNA targets. Analysis of the associative relationships of individual representatives of the selected pairs revealed that the level of mRNAs encoded by the ELN, ARL4C, C9orf64, PLAT, and FKBP9 genes associated with aggressive progression of glioma was increased in the IDHwt group. Meanwhile, the levels of miRNA-182, miRNA-455, and miRNA-891a associated with the negative prognosis in glioma were generally increased in the IDHmut group. Most (16/21) of the detected 21 microRNA-mRNA pairs with significant difference in regulation between IDHwt and IDHmut glioma samples had a weak or moderate positive correlation in IDHmut samples and a negative correlation in IDHwt samples. Therefore, our findings indicate that glioma samples from the IDHmut group with a positive prognosis potentially have a significantly less pronounced ability to microRNA-mediated regulation. We further suggest that such physiological disorders can lead to reduced tumor viability, resulting in an increased ability of the host to resist the spread of a malignant transformation of this genetic phenotype.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"38-45"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Characterization of Two Novel Lactococcus lactis Phages vL_296 and vL_20A. 两种新型乳球菌噬菌体 vL_296 和 vL_20A 的基因组特征。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27468

T A Chuksina, A A Fatkulin, N P Sorokina, I T Smykov, E V Kuraeva, E S Masagnaya, K A Smagina, M Yu Shkurnikov

{"title":"Genome Characterization of Two Novel Lactococcus lactis Phages vL_296 and vL_20A.","authors":"T A Chuksina, A A Fatkulin, N P Sorokina, I T Smykov, E V Kuraeva, E S Masagnaya, K A Smagina, M Yu Shkurnikov","doi":"10.32607/actanaturae.27468","DOIUrl":"10.32607/actanaturae.27468","url":null,"abstract":"Fermented dairy products are produced using starter cultures. They ferment milk to create products with a certain texture, aroma, and taste. However, the lactic acid bacteria used in this production are prone to bacteriophage infection. We examined the genomes of two newly discovered bacteriophage species that were isolated from cheese whey during the cheesemaking process. We have determined the species and the lytic spectrum of these bacteriophages. Phages vL_20A and vL_296 were isolated using lactococcal indicator cultures. They have unique lytic spectra: of the 21 possible identified host bacteria, only four are shared amongst them. The vL_20A and vL_296 genomes comprise linear double-stranded DNA lengths with 21,909 and 22,667 nucleotide pairs, respectively. Lactococcus phage bIL67 (ANI 93.3 and 92.6, respectively) is the closest to the phages vL_20A and vL_296. The analysis of the CRISPR spacers in the genomes of starter cultures did not reveal any phage-specific vL_20A or vL_296 among them. This study highlights the biodiversity of L. lactis phages, their widespread presence in dairy products, and their virulence. However, the virulence of phages is balanced by the presence of a significant number of bacterial strains with different sensitivities to phages in the starter cultures due to the bacterial immune system.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"102-109"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Features of Beta-Amyloid Phosphorylation in Alzheimer's Disease. 阿尔茨海默病的β-淀粉样蛋白磷酸化特征

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27456

P A Strelnikova, A E Bugrova, N V Zakharova, K V Danichkina, M I Indeykina, M S Gavrish, V G Krut', A A Babaev, A Yu Morozova, A S Kononikhin, V A Mitkevich, A A Makarov, E N Nikolaev

{"title":"The Features of Beta-Amyloid Phosphorylation in Alzheimer's Disease.","authors":"P A Strelnikova, A E Bugrova, N V Zakharova, K V Danichkina, M I Indeykina, M S Gavrish, V G Krut', A A Babaev, A Yu Morozova, A S Kononikhin, V A Mitkevich, A A Makarov, E N Nikolaev","doi":"10.32607/actanaturae.27456","DOIUrl":"10.32607/actanaturae.27456","url":null,"abstract":"Accumulation of neurotoxic aggregates of beta-amyloid peptides (Aβ) is a hallmark of Alzheimer's disease (AD) progression. Post-translational modifications (PTMs) increase Aβ aggregation and cytotoxicity, and the content of specific Aβ proteoforms is elevated in senile plaques of AD patients. The pathophysiological mechanisms of aggregate formation and the role of Aβ proteoforms need thorough study both to understand the role played by specific processes in the initiation of neuronal degradation and to find effective preventive means of therapeutic action. The present work investigates the dynamics of accumulation of phosphorylated serine-8 proteoform Aβ (pSer8-Aβ) using the 5xFAD mouse amyloid model. Aβ samples from human cerebrospinal fluid (CSF) and brain were also investigated. Western blot studies using 1E4E11 and 4G8 antibodies showed that accumulation of pSer8-Aβ in mouse brain starts as early as at the age of 3 months and reaches a maximum by the age of 14-17 months, which is generally similar to the dynamics of accumulation of the total pool of Aβ peptides. The pSer8-Aβ level in human CSF in AD patients can reach ~ 1-10% of the total amount of Aβ. Mass spectrometric analysis showed that Aβ phosphorylation by the Ser8, Tyr10, and Ser26 residues in brain tissues, as well as phosphorylation of the APP by Thr719 residue, is possible. These findings support the assumption that pSer8-Aβ proteoforms are involved in amyloidosis in AD. KEYWORDS Beta-amyloid, mass spectrometry, Alzheimer's disease, phosphorylation.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"93-101"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannula Implantation Reduces the Severity of the Beta Amyloid Effect on Peroxidized Lipids and Glutathione Levels in the Brain of BALB/c Mice. 植入插管可减轻 Beta 淀粉样蛋白对 BALB/c 小鼠脑内过氧化脂质和谷胱甘肽水平的影响。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27439

K A Mukhina, V A Mitkevich, I Yu Popova

{"title":"Cannula Implantation Reduces the Severity of the Beta Amyloid Effect on Peroxidized Lipids and Glutathione Levels in the Brain of BALB/c Mice.","authors":"K A Mukhina, V A Mitkevich, I Yu Popova","doi":"10.32607/actanaturae.27439","DOIUrl":"10.32607/actanaturae.27439","url":null,"abstract":"Sporadic Alzheimer's disease (sAD) is the most common of neurodegenerative disorders. The lack of effective therapy indicates that the mechanisms of sAD development remain poorly understood. To investigate this pathology in animals, intracerebroventricular injection of β-amyloid peptide (Aβ) using a Hamilton syringe, either during stereotactic surgery or through a pre-implanted cannula, is used. In this study, we analyzed the effect of chronic cannula implantation on the severity of Aβ effects at the behavioral, histological, and biochemical levels. The results showed that the local damage to neural tissue caused by cannulation has no bearing on the effect of Aβ on animal behavior and the microglial parameters of the unilateral hippocampus two weeks after the Aβ administration. However, cannula implantation fundamentally modifies some biochemical markers of the oxidative stress that occurs in the brain tissue in response to Aβ administration. Thus, the presence of a cannula reduces the severity of the Aβ impact on the levels of peroxidized lipids and glutathione two- and 10-fold, respectively. It is important to note that the detected changes are chronic and systemic. This is known because the homogenate of the entire contralateral (in relation to the cannula implantation site) hemisphere was analyzed, and the analysis was performed two weeks after implantation. At the same time, cannulation does not affect the rate of reactive oxygen species production. The obtained data indicate that chronic implantation of a cannula into the brain of experimental animals fundamentally distorts some parameters of oxidative stress in the neural tissue, which are widely used to assess the severity of experimental Alzheimer's-type diseases.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"51-59"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5'-Noraristeromycin Repurposing: Well-known S-Adenosyl-L-homocysteine Hydrolase Inhibitor As a Potential Drug Against Leukemia. 5'-Noraristeromycin Repurposing：知名的 S-腺苷-L-高半胱氨酸水解酶抑制剂作为抗白血病的潜在药物。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27443

O N Novikova, E S Matyugina, A V Gorshenin, Yu I Velikorodnaya, M D Krengauz, V O Vedernikova, P V Spirin, V S Prassolov, S N Kochetkov, A L Khandazhinskaya

引用次数: 0

Evolutionary Perspectives on Human-Artificial Intelligence Convergence. 人类与人工智能融合的进化视角。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27406

B L Zybailov, G Yu Kosovsky, G V Glazko, V I Glazko, O I Skobel

{"title":"Evolutionary Perspectives on Human-Artificial Intelligence Convergence.","authors":"B L Zybailov, G Yu Kosovsky, G V Glazko, V I Glazko, O I Skobel","doi":"10.32607/actanaturae.27406","DOIUrl":"10.32607/actanaturae.27406","url":null,"abstract":"In this analytical review, we explore the potential impact of the rapid proliferation of artificial intelligence (AI) tools on the biosphere and noosphere, suggesting that the trend may lead to a transformative event that could be termed \"Human-AI integration.\" We argue that this integration could give rise to novel lifeforms, associations, and hierarchies, resulting in competitive advantages and increased complexity of structural organizations within both the biosphere and noosphere. Our central premise emphasizes the importance of human-AI integration as a global adaptive response crucial for our civilization's survival amidst a rapidly changing environment. The convergence may initially manifest itself through symbiotic, endosymbiotic, or other mutualistic relationships, such as domestication, contingent on the rate at which AI systems achieve autonomy and develop survival instincts akin to those of biological organisms. We investigate potential drivers of these scenarios, addressing the ethical and existential challenges arising from the AI-driven transformation of the biosphere and noosphere, and considering potential trade-offs. Additionally, we discuss the application of complexity and the balance between competition and cooperation to better comprehend and navigate these transformative scenarios.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"4-17"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abundance of Tumor-Infiltrating B Cells in Human Epithelial Malignancies. 人类上皮恶性肿瘤中肿瘤浸润 B 细胞的丰富性

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27353

E A Petrov, D M Malabuiok, H Zheng, Yu A Mokrushina, V A Abrikosova, Yu B Kuzmin, P V Tzarapaev, S O Kochkina, I V Eltsov, V D Knorre, I V Smirnov, S S Terekhov, Z Mamedli, N E Kushlinskii, D V Rogozhin, V B Matveev, P V Kononets, I S Stilidi, H Zhang, A G Gabibov

{"title":"Abundance of Tumor-Infiltrating B Cells in Human Epithelial Malignancies.","authors":"E A Petrov, D M Malabuiok, H Zheng, Yu A Mokrushina, V A Abrikosova, Yu B Kuzmin, P V Tzarapaev, S O Kochkina, I V Eltsov, V D Knorre, I V Smirnov, S S Terekhov, Z Mamedli, N E Kushlinskii, D V Rogozhin, V B Matveev, P V Kononets, I S Stilidi, H Zhang, A G Gabibov","doi":"10.32607/actanaturae.27353","DOIUrl":"10.32607/actanaturae.27353","url":null,"abstract":"Cancer is a major global health problem. The type of malignant neoplasm and the potency of the immune response against tumors are two of the key factors influencing the outcome of the disease. The degree of tumor infiltration by lymphocytes plays an important role in antitumor response development, generally correlating with a favorable prognosis of treatment for certain cancers. We analyzed the abundance of tumor-infiltrating B cells (TIBs) in solid tumors of different cancers. TIBs were shown to be more abundant in colon and sigmoid colon cancer samples compared with cecal, rectal, and kidney cancer samples. The median and interquartile range of the TIB fraction were 11.5% and 4-20% in colon cancer, 6% and 3-11% in sigmoid colon cancer, 2.7% and 0.7-3.7% in cecal cancer, 2.5% and 0.9-3.6% in rectal cancer, 1.4% and 1.0-2.3% in kidney cancer, and 3.0% and 1.8-12% in lung cancer, respectively. However, there were no significant differences in the abundance of TIBs among samples at different stages of the cancer. Hence, investigation of the B cell response in colon cancer is of particular interest, since increased quantities of TIBs may indicate the existence of immunogenic tumor markers or the cell-cell interactions involved in disease progression. We believe that studying the diversity of TIBs in colon cancer will increaseour understanding of the mechanisms of the disease, contributing to the identification of new molecular targets for targeted oncotherapy.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"67-73"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Structure of the Components of the PolyADP-Ribosylation System in Fusarium Fungi and Evaluating the Expression Dynamics of Its Key Genes. 研究镰刀菌中 PolyADP-Ribosylation 系统的组成结构并评估其关键基因的表达动态。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27450

A A Stakheev, R R Kutukov, M E Taliansky, S K Zavriev

{"title":"Investigating the Structure of the Components of the PolyADP-Ribosylation System in Fusarium Fungi and Evaluating the Expression Dynamics of Its Key Genes.","authors":"A A Stakheev, R R Kutukov, M E Taliansky, S K Zavriev","doi":"10.32607/actanaturae.27450","DOIUrl":"10.32607/actanaturae.27450","url":null,"abstract":"Poly(ADP-ribose) polymerase (PARP) is the key enzyme in polyADP-ribosylation, one of the main post-translational modifications. This enzyme is abundant in eukaryotic organisms. However, information on the PARP structure and its functions in members of the Fungi kingdom is very limited. In this study, we performed a bioinformatic search for homologs of PARP and its antagonist, PARG, in the genomes of four Fusarium strains using their whole-genome sequences annotated and deposited in databases. The F. graminearum PH-1, F. proliferatum ET-1, and F. oxysporum Fo47 strains were shown to possess a single homolog of both PARP and PARG. In addition, the F. oxysporum f. sp. lycopersici strain 4287 contained four additional proteins comprising PARP catalytic domains whose structure was different from that of the remaining identified homologs. Partial nucleotide sequences encoding the catalytic domains of the PARP and PARG homologs were determined in 11 strains of 9 Fusarium species deposited in all-Russian collections, and the phylogenetic properties of the analyzed genes were evaluated. In the toxigenic F. graminearum strain, we demonstrated up-regulation of the gene encoding the PARP homolog upon culturing under conditions stimulating the production of the DON mycotoxin, as well as up-regulation of the gene encoding PARG at later stages of growth. These findings indirectly indicate involvement of the polyADP-ribosylation system in the regulation of the genes responsible for DON biosynthesis.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"83-92"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142650545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Activation of the Expression of Growth Factor Genes in Expi293F Human Cells Using CRISPR/Cas9-SAM Technology Increases Their Proliferation. 利用 CRISPR/Cas9-SAM 技术特异性激活 Expi293F 人体细胞中生长因子基因的表达可增加其增殖。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27415

P A Bobrovsky, E N Grafskaia, D D Kharlampieva, V A Manuvera, V N Lazarev

{"title":"Specific Activation of the Expression of Growth Factor Genes in Expi293F Human Cells Using CRISPR/Cas9-SAM Technology Increases Their Proliferation.","authors":"P A Bobrovsky, E N Grafskaia, D D Kharlampieva, V A Manuvera, V N Lazarev","doi":"10.32607/actanaturae.27415","DOIUrl":"10.32607/actanaturae.27415","url":null,"abstract":"Human cell lines play an important role in biotechnology and pharmacology. For them to grow, they need complex nutrient media containing signaling proteins - growth factors. We have tested a new approach that reduces the need of cultured human cell lines for exogenous growth factors. This approach is based on the generation of a modified cell with a selectively activated gene expression of one of the endogenous growth factors: IGF-1, FGF-2, or EIF3I. We modified the Expi293F cell line, a HEK293 cell line variant widely used in the production of recombinant proteins. Gene expression of the selected growth factors in these cells was activated using CRISPR/Cas9 technology with the synergistic activation mediators CRISPR/Cas9-SAM, which increased the expression of the selected genes at both mRNA and protein levels. Upon culturing under standard conditions, the modified lines exhibited increased proliferation. A synergistic effect was observed in co-culture of the three modified lines. In our opinion, these results indicate that this approach is promising for efficient modification of cell lines used in biotechnology.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"25-37"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0