Acta Naturae最新文献_第2页

A Targeted Liposomal Delivery System for Hydrophobic Antitumor Compounds Using the Example of a 1,2,3,4-Tetrahydroisoquinoline Derivative. 以1,2,3,4-四氢异喹啉衍生物为例的疏水抗肿瘤化合物的靶向脂质体递送系统。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-07-01 DOI: 10.32607/actanaturae.28027

G M Proshkina, O N Ashirov, M N Zharkov, A Al-Khadj Aioub, V I Vinogradova, D Zhu, R Yan, Sh S Azimova, E I Shramova, N A Pyataev, S M Deyev

{"title":"A Targeted Liposomal Delivery System for Hydrophobic Antitumor Compounds Using the Example of a 1,2,3,4-Tetrahydroisoquinoline Derivative.","authors":"G M Proshkina, O N Ashirov, M N Zharkov, A Al-Khadj Aioub, V I Vinogradova, D Zhu, R Yan, Sh S Azimova, E I Shramova, N A Pyataev, S M Deyev","doi":"10.32607/actanaturae.28027","DOIUrl":"https://doi.org/10.32607/actanaturae.28027","url":null,"abstract":"Cancer is the second leading cause of death worldwide, after cardiovascular disorders. One of the main constraints of anticancer agents is their limited water solubility, which complicates preclinical in vivo studies. In this work, we propose a versatile approach for stabilizing hydrophobic antitumor agents using liposomes based on natural phospholipids. The efficacy of the method is demonstrated for hydrophobic 1-tridecyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline, a promising tetrahydroisoquinoline derivative with proven antitumor activity against lymphoid and epithelial cancer cells. We show that the liposomal formulation ensures drug stability in aqueous media. Modification of the liposome surface with a HER2-specific non-immunoglobulin module strongly improved targeting efficiency. The targeting index differed markedly between cells with low versus high HER2 expression levels (3.02 and 18.98, respectively), demonstrating that the targeted liposomal formulation exerts a substantially enhanced cytotoxicity against HER2-overexpressing cells. The proposed liposomal platform can also be utilized to deliver other hydrophobic anticancer drugs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 3","pages":"102-108"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13719161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulation of Expression of Nicotinic Acetylcholine Receptors and Ly6/uPAR Proteins in the Cingulate Gyrus of the Cerebral Cortex of Patients with Alzheimer's Disease. 阿尔茨海默病患者大脑皮层扣带回中烟碱乙酰胆碱受体和Ly6/uPAR蛋白表达失调

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-07-01 DOI: 10.32607/actanaturae.27904

M L Bychkov, A G Ochneva, V M Ushakova, A Yu Morozova, Ya A Zorkina, O V Abramova, G P Kostyuk, M P Kirpichnikov, A S Paramonov, E N Lyukmanova

{"title":"Dysregulation of Expression of Nicotinic Acetylcholine Receptors and Ly6/uPAR Proteins in the Cingulate Gyrus of the Cerebral Cortex of Patients with Alzheimer's Disease.","authors":"M L Bychkov, A G Ochneva, V M Ushakova, A Yu Morozova, Ya A Zorkina, O V Abramova, G P Kostyuk, M P Kirpichnikov, A S Paramonov, E N Lyukmanova","doi":"10.32607/actanaturae.27904","DOIUrl":"https://doi.org/10.32607/actanaturae.27904","url":null,"abstract":"Alzheimer's disease (AD) is a common neurodegenerative disorder associated with the dysfunction of nicotinic acetylcholine receptors (nAChRs). The function of nAChRs is normally regulated by endogenous ligands, such as acetylcholine and three-finger proteins belonging to the Ly6/uPAR family. However, information on the involvement of these proteins in the pathogenesis of AD is lacking. In this study, by using a bioinformatic analysis, we identified increased mRNA levels of Ly6/uPAR proteins (Lynx1, Lynx2, SLURP-1, Ly6G6E, etc.) in the entorhinal, temporal, and frontal cortex in AD patients with neurodegenerative symptoms, as well as an increase in the mRNA level of Ly6E in the hippocampus in patients with asymptomatic AD. Furthermore, in the cortex and cerebellum, we identified multidirectional changes in the mRNA expression of different nAChR subunits and regulators of inflammation (CD59, CD177, Lypd2, Lypd4, and Lypd8) and metabolism (GPIHBP and Ly6D), also belonging to the Ly6/uPAR protein family. By using immunostaining and Western blotting, we confirmed increased protein levels of Lynx1, Lynx2, and the β2 nAChR subunit in the cingulate gyrus adjacent to the entorhinal cortex in patients with AD, as well as decreased protein levels of the α4 and α7 nAChR subunits. These findings point to complex dysregulation of the cholinergic system in the AD brain at the expression levels both of the individual nAChR subtypes and its natural modulators belonging to the Ly6/uPAR family.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 3","pages":"48-55"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13719160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Microbial Transglutaminase for the Production of Homogeneous Immunotherapeutic Antibody-Drug Conjugates. 微生物转谷氨酰胺酶的固定化生产均质免疫治疗抗体-药物偶联物。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-07-01 DOI: 10.32607/actanaturae.27926

M N Baranova, K A Sapozhnikova, E L Gulyak, Y A Prokopenko, O A Komarova, Yu A Mokrushina, I V Smirnov, V A Korshun, S S Terekhov

{"title":"Immobilization of Microbial Transglutaminase for the Production of Homogeneous Immunotherapeutic Antibody-Drug Conjugates.","authors":"M N Baranova, K A Sapozhnikova, E L Gulyak, Y A Prokopenko, O A Komarova, Yu A Mokrushina, I V Smirnov, V A Korshun, S S Terekhov","doi":"10.32607/actanaturae.27926","DOIUrl":"https://doi.org/10.32607/actanaturae.27926","url":null,"abstract":"Antibody-drug conjugates (ADCs) have revolutionized chemotherapy, allowing it to shift from systemic cytotoxic chemotherapy to specific targeting of cells expressing tumor antigens. ADCs thus offer increased therapeutic efficacy and lower toxicity compared to conventional chemotherapy. Bioconjugation of a highly active therapeutic agent to an antibody is one of the key steps in producing ADCs. The most advanced ADC technologies are based on the production of stoichiometrically homogeneous bioconjugates. Enzymatic modification of antibodies using microbial transglutaminase (mTGase) enables the production of biopharmaceuticals with standardized pharmacokinetic, physicochemical, and therapeutic properties. However, side reactions catalyzed by free mTGase in solution reduce the yield and purity of ADCs. In this study, we evaluated the efficiency of various approaches to mTGase immobilization: streptavidin-biotin interaction, the use of an epoxy-activated polymer, and self-immobilization of mTGase. The highest bioconjugation activity was observed when using streptavidin-modified magnetic particles and biotinylated mTGase. However, mTGase self-immobilization on AH-Sepharose 4B or immobilization using epoxy-activated agarose ensures comparable efficiency in enzymatic antibody modification and is the most rational route from the perspective of process scalability. We believe that the proposed approaches are of high practical significance, opening up avenues for optimizing enzymatic bioconjugation and scaling up the production of stoichiometric ADCs for targeted cancer immunotherapy.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 3","pages":"42-47"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13719159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long Non-Coding RNAs: Allies and Enemies of HIV Infection. 长链非编码rna: HIV感染的盟友和敌人。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27881

T F Kikhai, A V Mashkovskaia, T S Oretskaya, Y Y Agapkina, M B Gottikh

引用次数: 0

Isolation of Monoclonal Neutralizing Single-Domain Antibodies Against Clostridioides difficile Toxin B. 艰难梭菌毒素B单克隆中和性单域抗体的分离。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27874

I A Alekseeva, A S Ungur, I A Favorskaya, A I Tukhvatulin, D V Shcheblyakov, A I Korobkova, M E Komyakova, O L Voronina, N N Ryzhova, E I Ermolova, M S Kunda, D Yu Logunov, M M Bobrova, V V Makarov, S M Yudin, A L Gintsburg

引用次数: 0

A Comparative Study of Genital Lichen Sclerosus Transcriptomes. 生殖硬化地衣转录组的比较研究。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27889

S D Margasyuk, A L Kuznetsova, D A Skvortsov, E M Alekberov, M M Iritsyan, S A Pulbere, S V Kotov, A A Sokolova, D D Pervouchine

{"title":"A Comparative Study of Genital Lichen Sclerosus Transcriptomes.","authors":"S D Margasyuk, A L Kuznetsova, D A Skvortsov, E M Alekberov, M M Iritsyan, S A Pulbere, S V Kotov, A A Sokolova, D D Pervouchine","doi":"10.32607/actanaturae.27889","DOIUrl":"10.32607/actanaturae.27889","url":null,"abstract":"Genital lichen sclerosus (GLS) is a chronic inflammatory dermatosis that affects the genital skin. Despite different clinical manifestations, the pathogenesis of GLS in men and women is believed to be common and is attributed to a combination of autoimmune and genetic factors. In this study, we compared the transcriptomic profiles of penile (mGLS) and vulvar lichen sclerosus (VLS), aiming to identify commonly deregulated genes. We observed a substantial heterogeneity in the transcriptomic signatures in mGLS samples, which is driven by different compositions of immune infiltrates. In mGLS, gene expression signatures strongly indicate epidermis dysfunction and overexpression of the epithelial inflammation marker Keratin 6 (KRT6) and chitinase CHIT1. No significant changes in the expression levels of known GLS markers, such as VIM, CTNNB1, LGALS7 and ECM1, were detected. However, significant changes in the expression levels of the genes associated with autoimmune diseases and the genes upregulated in squamous cell carcinoma, including TNF, CCNB1 and RUNX3, were observed. There was no enrichment in the polyU/UC insertions that were reported previously. Instead, we have identified a long non-coding RNA DRAIC with a high coding potential that is commonly upregulated in mGLS and VLS. Taken together, our results provide a comprehensive picture of the shared transcriptomic signatures, including novel biomarkers and potential therapeutic targets.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 2","pages":"85-96"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Transcriptional Kinases CDK8/19 in the Regulation of the Macrophage Inflammatory Response. 转录激酶CDK8/19在巨噬细胞炎症反应中的调控作用。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27815

A N Neznamov, Yu A Oparina, D M Dolmatova, L A Ilchuk, M V Kubekina

{"title":"The Transcriptional Kinases CDK8/19 in the Regulation of the Macrophage Inflammatory Response.","authors":"A N Neznamov, Yu A Oparina, D M Dolmatova, L A Ilchuk, M V Kubekina","doi":"10.32607/actanaturae.27815","DOIUrl":"10.32607/actanaturae.27815","url":null,"abstract":"Macrophage dysfunction is a key pathogenetic mechanism in the progression of a wide range of human chronic inflammation conditions, including atherosclerosis, rheumatoid arthritis, and metabolic disorders. The CDK8 and CDK19 paralogous kinases, the subunits of the Mediator complex acting as transcription regulators, are essential modulators of the inflammatory response. This study addresses the role of CDK8/19 kinases in the macrophage inflammatory response via multiple mechanisms: activation of pro- and anti-inflammatory genes and surface markers, STAT1 pathway modulation, activation of glycolytic cascade genes, regulation of the dynamics of lipid inclusions, and phagocytic activity. Experiments have shown that CDK8 and CDK19 may exhibit functional divergence. Cdk19 knockout reveals that CDK19 plays a role in the suppression of the M1 response, as well as the regulation of lipid homeostasis and phagocytosis. Double Cdk8/19 knockout macrophages are characterized by an exacerbated anti-inflammatory response while preserving normal lipid accumulation and phagocytosis levels. Inhibition of CDK8/19 kinase activity reproduces these effects only partially, suggesting that both kinase-dependent and kinase-independent mechanisms of action exist. The identified effects reveal previously unknown regulation mechanisms of macrophage immunometabolism. Modulation of CDK8 and CDK19 activity can become a novel therapeutic target for a wide range of chronic inflammatory conditions and metabolic disorders associated with macrophage dysfunction.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 2","pages":"97-107"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COGcollator 2.0: A Tool for Analyzing Distant Evolutionary Relationships Among Families of Homologous Proteins. COGcollator 2.0：一个分析同源蛋白家族间遥远进化关系的工具。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27789

D V Dibrova, S Y Rykov

{"title":"COGcollator 2.0: A Tool for Analyzing Distant Evolutionary Relationships Among Families of Homologous Proteins.","authors":"D V Dibrova, S Y Rykov","doi":"10.32607/actanaturae.27789","DOIUrl":"10.32607/actanaturae.27789","url":null,"abstract":"The analysis of protein evolutionary relationships is of both theoretical and practical significance. Identifying and characterizing evolutionary relationships between the components of protein complexes and metabolic enzymes fosters a deeper understanding of their evolutionary history and molecular evolution. The existence of related enzymes makes the annotation of new sequences complicated, since this process relies heavily on accurately determining the protein family classification of the sequence being analyzed. Clusters of Orthologous Groups (COGs) are widely used for the classification of prokaryotic proteins. COGs are constructed based on the occurrence of the respective protein-coding genes within complete genomes. Previously, we introduced COGcollator, a tool designed to visualize the relatedness between COGs by analyzing the hits of their profile HMMs (Hidden Markov Models). This paper presents an update of the COGcollator web service. It is based on the latest version of the COG database and features a completely new interface and additional functionalities. To demonstrate the capabilities of our tool and the validity of the data, we present the COGcollator results for the subunits of NADH:quinone oxidoreductase type 1 (NDH-1), a homologue of the mitochondrial complex I, as the evolutionary relationships of NDH-1 with other protein complexes have been extensively documented in the literature. The web service is available free of charge without registration at https://boabio.belozersky.msu.ru/en/COGcollator. Through the web service interface, users can access pre-calculated COGcollator results for 4,972 COGs and download their respective profile HMMs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 2","pages":"45-53"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Determinants of Cellular Homeostasis Contributing to the Risk of Type 2 Diabetes. 细胞内稳态与2型糖尿病风险相关的遗传决定因素

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27855

Y R Timasheva, O V Kochetova, T R Nasibullin, T M Kochetova, Z Balkhiyarova, G F Korytina, A Nouwen

{"title":"Genetic Determinants of Cellular Homeostasis Contributing to the Risk of Type 2 Diabetes.","authors":"Y R Timasheva, O V Kochetova, T R Nasibullin, T M Kochetova, Z Balkhiyarova, G F Korytina, A Nouwen","doi":"10.32607/actanaturae.27855","DOIUrl":"10.32607/actanaturae.27855","url":null,"abstract":"Genetic variation in the genes involved in the maintenance of cellular homeostasis may influence susceptibility to type 2 diabetes (T2D). However, the nature of the interplay among these loci and their prognostic value remains unclear. In this study, we analyzed variants in the FOXO3A, FOXO3, FOXO1, HMOX1, and SIRT1 genes. The HMOX1 rs2071746*T/T genotype was associated with an increased risk of T2D (OR = 1.36, PFDR = 0.030), whereas the FOXO1 rs9549240 (OR = 0.52, PFDR = 0.002) and SIRT1 rs3758391 (OR = 0.80, PFDR = 0.015) genotypes exhibited a protective effect. We detected nonlinear interactions, including FOXO1 rs9549240*G + SIRT1 rs3758391*T + SIRT1 rs7895833*A and HMOX1 rs2071746*A + SIRT1 rs3758391*T + SIRT1 rs7895833*A, with significant synergistic effects (SF = 3.19 and 2.56, P < 0.03). Models incorporating these interactions achieved an AUC of 69.8%, which increased to 86.2% when combined with age, sex, and the body mass index. These findings suggest that interactions between the pathways regulating oxidative stress and metabolism may contribute to the genetic predisposition to T2D.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 2","pages":"108-115"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Endocytosis Inhibitors on the Cytotoxicity and Antitumor Activity of Anti-GD2 ADCs. 内吞抑制剂对抗gd2 adc细胞毒性和抗肿瘤活性的影响。

IF 1.5 4区生物学

Acta Naturae Pub Date : 2026-04-01 DOI: 10.32607/actanaturae.27900

M M Titov, I V Kholodenko, D V Kalinovsky, A O Makarova, D Y Ryazantsev, E V Svirshchevskaya, S M Deyev, R V Kholodenko

{"title":"Effect of Endocytosis Inhibitors on the Cytotoxicity and Antitumor Activity of Anti-GD2 ADCs.","authors":"M M Titov, I V Kholodenko, D V Kalinovsky, A O Makarova, D Y Ryazantsev, E V Svirshchevskaya, S M Deyev, R V Kholodenko","doi":"10.32607/actanaturae.27900","DOIUrl":"10.32607/actanaturae.27900","url":null,"abstract":"Cancer remains a critical public health challenge, and developing novel approaches to cancer therapy is highly relevant. Targeted tumor therapy using antibody-drug conjugates (ADCs) has already demonstrated its efficacy in multiple tumors. Ganglioside GD2 is a promising target for ADC development. However, its functional properties, which are important for the cytotoxicity of ADCs, remain poorly understood. This study focuses on the mechanisms of receptor-mediated endocytosis for the conjugate formed between the anti-GD2 antibody ch14.18 and monomethyl auristatin E (MMAE), as well as the approaches for modulating this process using endocytosis inhibitors. Our findings demonstrate that anti-GD2 ADCs are efficiently internalized into tumor cells through various endocytic pathways, including the clathrin- and caveolin-mediated pathways, as well as macropinocytosis. The efficiency of ADC accumulation directly correlates with the level of GD2 expression on the tumor cell surface and is determined by the properties of the parental antibody, independent of the cytotoxic payload. Endocytosis inhibitors can modulate the functional properties of anti-GD2 ADCs, either decreasing or increasing the cytotoxic effects of the conjugates in GD2-positive cells. Nystatin, a specific inhibitor of caveolin-mediated endocytosis, increased ADC accumulation in cells and reduced the IC50 1.5- to 2.5-fold depending on the cell line. Although administration of nystatin together with anti-GD2 ADCs did not enhance tumor growth inhibition compared to ADC monotherapy in the GD2- positive mouse tumor model, further optimization of combination therapy conditions aimed at enhancing receptor-mediated endocytosis is a promising strategy for potentiating the antitumor effects of anti-GD2 ADCs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"18 2","pages":"116-126"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0