Acta Naturae最新文献_第3页

The Characteristics of the Metabolomic Profile in Patients with Parkinson's Disease and Vascular Parkinsonism. 帕金森病和血管性帕金森病患者的代谢组学特征

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27511

E V Predtechenskaya, A D Rogachev, P M Melnikova

{"title":"The Characteristics of the Metabolomic Profile in Patients with Parkinson's Disease and Vascular Parkinsonism.","authors":"E V Predtechenskaya, A D Rogachev, P M Melnikova","doi":"10.32607/actanaturae.27511","DOIUrl":"10.32607/actanaturae.27511","url":null,"abstract":"The gradually increasing age of the world population implies that the prevalence of neurodegenerative diseases also continues to rise. These diseases are characterized by a progressive loss of cognitive and motor functions. Parkinson's disease, which involves the gradual death of specialized neural tissue, is a striking example of a neurodegenerative process. The pathomorphological analysis shows that chronic cerebral ischemia is accompanied by extensive complex neurodegeneration; parkinsonism is its clinical manifestation in 20-30% of cases. Although Parkinson's disease and vascular parkinsonism are similar, these two pathologies have fundamentally different etiopathogeneses. But their set of differential diagnosis traits is confined to some features of the neurological status. There currently exist no diagnostic markers for individual neurodegenerative pathologies or the neurodegeneration phenomenon in general. Metabolomic profiling can be a promising means for finding a unique \"fingerprint\" of the disease. Identifying the biomarkers of various neurodegenerative diseases will help shorten the time to the diagnosis, forecast the course of the disease, and personalize the therapeutic approach. This review summarizes and compares the current concepts of metabolomics research into Parkinson's disease and vascular parkinsonism, as well as the respective animal models.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 4","pages":"27-37"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide Mimicking Loop II of the Human Epithelial Protein SLURP-2 Enhances the Viability and Migration of Skin Keratinocytes. 人上皮蛋白SLURP-2的肽模拟环II增强皮肤角质形成细胞的活力和迁移。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27494

O V Shlepova, T Ya Gornostaeva, I D Kukushkin, V N Azev, M L Bychkov, Z O Shenkarev, M P Kirpichnikov, E N Lyukmanova

{"title":"Peptide Mimicking Loop II of the Human Epithelial Protein SLURP-2 Enhances the Viability and Migration of Skin Keratinocytes.","authors":"O V Shlepova, T Ya Gornostaeva, I D Kukushkin, V N Azev, M L Bychkov, Z O Shenkarev, M P Kirpichnikov, E N Lyukmanova","doi":"10.32607/actanaturae.27494","DOIUrl":"10.32607/actanaturae.27494","url":null,"abstract":"The secreted human protein SLURP-2 is a regulator of epithelial homeostasis, which enhances the viability and migration of keratinocytes. The targets of SLURP-2 in keratinocytes are nicotinic and muscarinic acetylcholine receptors. This work is devoted to the search for the SLURP-2 functional regions responsible for enhancing keratinocyte viability and migration. We produced synthetic peptides corresponding to the SLURP-2 loop regions and studied their effect on the viability and migration of HaCaT skin keratinocytes using the WST-8 test and scratch-test, respectively. The highest activity was exhibited by a loop II-mimicking peptide that enhanced the viability of keratinocytes and stimulated their migration. The peptide activity was mediated by interactions with α7- and α3β2-nAChRs and suppression of the p38 MAPK intracellular signaling pathway. Thus, we obtained new data that explain the mechanisms underlying SLURP-2 regulatory activity and indicate the promise of further research into loop II-mimicking peptides as prototypes of wound healing drugs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 4","pages":"86-94"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive Byproducts of Plant Redox Metabolism and Protein Functions. 植物氧化还原代谢反应副产物与蛋白质功能。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27477

E I Sharova, S S Medvedev

{"title":"Reactive Byproducts of Plant Redox Metabolism and Protein Functions.","authors":"E I Sharova, S S Medvedev","doi":"10.32607/actanaturae.27477","DOIUrl":"10.32607/actanaturae.27477","url":null,"abstract":"Living organisms exhibit an impressive ability to expand the basic information encoded in their genome, specifically regarding the structure and function of protein. Two basic strategies are employed to increase protein diversity and functionality: alternative mRNA splicing and post-translational protein modifications (PTMs). Enzymatic regulation is responsible for the majority of the chemical reactions occurring within living cells. However, plants redox metabolism perpetually generates reactive byproducts that spontaneously interact with and modify biomolecules, including proteins. Reactive carbonyls resulted from the oxidative metabolism of carbohydrates and lipids carbonylate proteins, leading to the latter inactivation and deposition in the form of glycation and lipoxidation end products. The protein nitrosylation caused by reactive nitrogen species plays a crucial role in plant morphogenesis and stress reactions. The redox state of protein thiol groups modified by reactive oxygen species is regulated through the interplay of thioredoxins and glutaredoxins, thereby influencing processes such as protein folding, enzyme activity, and calcium and hormone signaling. This review provides a summary of the PTMs caused by chemically active metabolites and explores their functional consequences in plant proteins.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 4","pages":"48-61"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Spacer Targets in CRISPR-Cas Systems of Starter Cultures. 发酵剂CRISPR-Cas系统中间隔靶点的比较分析。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27533

A A Fatkulin, T A Chuksina, N P Sorokina, I T Smykov, E V Kuraeva, E S Masezhnaya, K A Smagina, M Yu Shkurnikov

引用次数: 0

Insights into the Functioning of the D-amino Acid Transaminase from Haliscomenobacter Hydrossis via a Structural and Spectral Analysis of its Complex with 3-Aminooxypropionic Acid. 通过对 Haliscomenobacter Hydrossis D-amino Acid Transaminase 与 3-Aminooxypropionic Acid 复合物的结构和光谱分析了解其功能。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27496

A K Bakunova, I O Matyuta, A Yu Nikolaeva, K M Boyko, A R Khomutov, E Yu Bezsudnova, V O Popov

{"title":"Insights into the Functioning of the D-amino Acid Transaminase from Haliscomenobacter Hydrossis via a Structural and Spectral Analysis of its Complex with 3-Aminooxypropionic Acid.","authors":"A K Bakunova, I O Matyuta, A Yu Nikolaeva, K M Boyko, A R Khomutov, E Yu Bezsudnova, V O Popov","doi":"10.32607/actanaturae.27496","DOIUrl":"10.32607/actanaturae.27496","url":null,"abstract":"Pyridoxal 5'-phosphate-dependent enzymes play a crucial role in nitrogen metabolism. Carbonyl compounds, such as O-substituted hydroxylamines, stand out among numerous specific inhibitors of these enzymes, including those of practical importance, because they react with pyridoxal 5'-phosphate in the active site of the enzymes to form stable oximes. O-substituted hydroxylamines mimic the side group of amino acid substrates, thus providing highly potent and specific inhibition of the corresponding enzymes. The interaction between D-amino acid transaminase from bacterium Haliscomenobacter hydrossis and 3-aminooxypropionic acid was studied in the present work. The structural and spectral analyses of the complex of this transaminase with 3-aminooxypropionic acid allowed us to clarify some features of the organization and functioning of its active site and illustrate one of the mechanisms of inhibition by the specific substrate, D-glutamic acid.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"18-24"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Correlation Patterns of miRNA Expression with Targeted mRNA Transcripts in Glioma Patients with Wild-Type and Mutated Isocitrate Dehydrogenase (IDH) Genotypes. 野生型和变异型异柠檬酸脱氢酶（IDH）基因型胶质瘤患者体内 miRNA 表达与靶向 mRNA 转录本的相关模式

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27363

A A Bondarev, A S Evpak, A L Novoselov, A A Kudriaeva, A A Jr Belogurov

{"title":"The Correlation Patterns of miRNA Expression with Targeted mRNA Transcripts in Glioma Patients with Wild-Type and Mutated Isocitrate Dehydrogenase (IDH) Genotypes.","authors":"A A Bondarev, A S Evpak, A L Novoselov, A A Kudriaeva, A A Jr Belogurov","doi":"10.32607/actanaturae.27363","DOIUrl":"10.32607/actanaturae.27363","url":null,"abstract":"Low-grade gliomas are divided into two main genetic phenotypes based on the presence or absence of mutations in the isocitrate dehydrogenase (IDH) genes. The mutated IDH phenotype (IDHmut), in contrast to the wild-type phenotype (IDHwt), is characterized by a more positive response to pharmacological intervention and a significantly longer survival time. In this study, we analyzed the differential co-expression of 225,000 microRNA-mRNA pairs at the level of correlations between microRNA levels and their potential mRNA targets. Analysis of the associative relationships of individual representatives of the selected pairs revealed that the level of mRNAs encoded by the ELN, ARL4C, C9orf64, PLAT, and FKBP9 genes associated with aggressive progression of glioma was increased in the IDHwt group. Meanwhile, the levels of miRNA-182, miRNA-455, and miRNA-891a associated with the negative prognosis in glioma were generally increased in the IDHmut group. Most (16/21) of the detected 21 microRNA-mRNA pairs with significant difference in regulation between IDHwt and IDHmut glioma samples had a weak or moderate positive correlation in IDHmut samples and a negative correlation in IDHwt samples. Therefore, our findings indicate that glioma samples from the IDHmut group with a positive prognosis potentially have a significantly less pronounced ability to microRNA-mediated regulation. We further suggest that such physiological disorders can lead to reduced tumor viability, resulting in an increased ability of the host to resist the spread of a malignant transformation of this genetic phenotype.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"38-45"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Characterization of Two Novel Lactococcus lactis Phages vL_296 and vL_20A. 两种新型乳球菌噬菌体 vL_296 和 vL_20A 的基因组特征。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27468

T A Chuksina, A A Fatkulin, N P Sorokina, I T Smykov, E V Kuraeva, E S Masagnaya, K A Smagina, M Yu Shkurnikov

{"title":"Genome Characterization of Two Novel Lactococcus lactis Phages vL_296 and vL_20A.","authors":"T A Chuksina, A A Fatkulin, N P Sorokina, I T Smykov, E V Kuraeva, E S Masagnaya, K A Smagina, M Yu Shkurnikov","doi":"10.32607/actanaturae.27468","DOIUrl":"10.32607/actanaturae.27468","url":null,"abstract":"Fermented dairy products are produced using starter cultures. They ferment milk to create products with a certain texture, aroma, and taste. However, the lactic acid bacteria used in this production are prone to bacteriophage infection. We examined the genomes of two newly discovered bacteriophage species that were isolated from cheese whey during the cheesemaking process. We have determined the species and the lytic spectrum of these bacteriophages. Phages vL_20A and vL_296 were isolated using lactococcal indicator cultures. They have unique lytic spectra: of the 21 possible identified host bacteria, only four are shared amongst them. The vL_20A and vL_296 genomes comprise linear double-stranded DNA lengths with 21,909 and 22,667 nucleotide pairs, respectively. Lactococcus phage bIL67 (ANI 93.3 and 92.6, respectively) is the closest to the phages vL_20A and vL_296. The analysis of the CRISPR spacers in the genomes of starter cultures did not reveal any phage-specific vL_20A or vL_296 among them. This study highlights the biodiversity of L. lactis phages, their widespread presence in dairy products, and their virulence. However, the virulence of phages is balanced by the presence of a significant number of bacterial strains with different sensitivities to phages in the starter cultures due to the bacterial immune system.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"102-109"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Features of Beta-Amyloid Phosphorylation in Alzheimer's Disease. 阿尔茨海默病的β-淀粉样蛋白磷酸化特征

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27456

P A Strelnikova, A E Bugrova, N V Zakharova, K V Danichkina, M I Indeykina, M S Gavrish, V G Krut', A A Babaev, A Yu Morozova, A S Kononikhin, V A Mitkevich, A A Makarov, E N Nikolaev

{"title":"The Features of Beta-Amyloid Phosphorylation in Alzheimer's Disease.","authors":"P A Strelnikova, A E Bugrova, N V Zakharova, K V Danichkina, M I Indeykina, M S Gavrish, V G Krut', A A Babaev, A Yu Morozova, A S Kononikhin, V A Mitkevich, A A Makarov, E N Nikolaev","doi":"10.32607/actanaturae.27456","DOIUrl":"10.32607/actanaturae.27456","url":null,"abstract":"Accumulation of neurotoxic aggregates of beta-amyloid peptides (Aβ) is a hallmark of Alzheimer's disease (AD) progression. Post-translational modifications (PTMs) increase Aβ aggregation and cytotoxicity, and the content of specific Aβ proteoforms is elevated in senile plaques of AD patients. The pathophysiological mechanisms of aggregate formation and the role of Aβ proteoforms need thorough study both to understand the role played by specific processes in the initiation of neuronal degradation and to find effective preventive means of therapeutic action. The present work investigates the dynamics of accumulation of phosphorylated serine-8 proteoform Aβ (pSer8-Aβ) using the 5xFAD mouse amyloid model. Aβ samples from human cerebrospinal fluid (CSF) and brain were also investigated. Western blot studies using 1E4E11 and 4G8 antibodies showed that accumulation of pSer8-Aβ in mouse brain starts as early as at the age of 3 months and reaches a maximum by the age of 14-17 months, which is generally similar to the dynamics of accumulation of the total pool of Aβ peptides. The pSer8-Aβ level in human CSF in AD patients can reach ~ 1-10% of the total amount of Aβ. Mass spectrometric analysis showed that Aβ phosphorylation by the Ser8, Tyr10, and Ser26 residues in brain tissues, as well as phosphorylation of the APP by Thr719 residue, is possible. These findings support the assumption that pSer8-Aβ proteoforms are involved in amyloidosis in AD. KEYWORDS Beta-amyloid, mass spectrometry, Alzheimer's disease, phosphorylation.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"93-101"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannula Implantation Reduces the Severity of the Beta Amyloid Effect on Peroxidized Lipids and Glutathione Levels in the Brain of BALB/c Mice. 植入插管可减轻 Beta 淀粉样蛋白对 BALB/c 小鼠脑内过氧化脂质和谷胱甘肽水平的影响。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27439

K A Mukhina, V A Mitkevich, I Yu Popova

{"title":"Cannula Implantation Reduces the Severity of the Beta Amyloid Effect on Peroxidized Lipids and Glutathione Levels in the Brain of BALB/c Mice.","authors":"K A Mukhina, V A Mitkevich, I Yu Popova","doi":"10.32607/actanaturae.27439","DOIUrl":"10.32607/actanaturae.27439","url":null,"abstract":"Sporadic Alzheimer's disease (sAD) is the most common of neurodegenerative disorders. The lack of effective therapy indicates that the mechanisms of sAD development remain poorly understood. To investigate this pathology in animals, intracerebroventricular injection of β-amyloid peptide (Aβ) using a Hamilton syringe, either during stereotactic surgery or through a pre-implanted cannula, is used. In this study, we analyzed the effect of chronic cannula implantation on the severity of Aβ effects at the behavioral, histological, and biochemical levels. The results showed that the local damage to neural tissue caused by cannulation has no bearing on the effect of Aβ on animal behavior and the microglial parameters of the unilateral hippocampus two weeks after the Aβ administration. However, cannula implantation fundamentally modifies some biochemical markers of the oxidative stress that occurs in the brain tissue in response to Aβ administration. Thus, the presence of a cannula reduces the severity of the Aβ impact on the levels of peroxidized lipids and glutathione two- and 10-fold, respectively. It is important to note that the detected changes are chronic and systemic. This is known because the homogenate of the entire contralateral (in relation to the cannula implantation site) hemisphere was analyzed, and the analysis was performed two weeks after implantation. At the same time, cannulation does not affect the rate of reactive oxygen species production. The obtained data indicate that chronic implantation of a cannula into the brain of experimental animals fundamentally distorts some parameters of oxidative stress in the neural tissue, which are widely used to assess the severity of experimental Alzheimer's-type diseases.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"51-59"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5'-Noraristeromycin Repurposing: Well-known S-Adenosyl-L-homocysteine Hydrolase Inhibitor As a Potential Drug Against Leukemia. 5'-Noraristeromycin Repurposing：知名的 S-腺苷-L-高半胱氨酸水解酶抑制剂作为抗白血病的潜在药物。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27443

O N Novikova, E S Matyugina, A V Gorshenin, Yu I Velikorodnaya, M D Krengauz, V O Vedernikova, P V Spirin, V S Prassolov, S N Kochetkov, A L Khandazhinskaya

引用次数: 0