Acta Naturae最新文献_第3页

Evaluation of HER2/neu Expression in Metastatic Axillary Lymph Node Tissue of Breast Cancer Patients Using [99mTc]Tc-(HE)3-G3. 使用[99m锝]锝-(HE)3-G3评估乳腺癌患者转移性腋窝淋巴结组织中HER2/neu的表达。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-04-01 DOI: 10.32607/actanaturae.27448

O D Bragina, L A Tashireva, D M Loos, S V Vtorushin, A A Shulga, E N Konovalova, M E Borodina, V I Chernov, V M Tolmachev, S M Deyev

{"title":"Evaluation of HER2/neu Expression in Metastatic Axillary Lymph Node Tissue of Breast Cancer Patients Using [99mTc]Tc-(HE)3-G3.","authors":"O D Bragina, L A Tashireva, D M Loos, S V Vtorushin, A A Shulga, E N Konovalova, M E Borodina, V I Chernov, V M Tolmachev, S M Deyev","doi":"10.32607/actanaturae.27448","DOIUrl":"10.32607/actanaturae.27448","url":null,"abstract":"Anatomic visualization and molecular typing of metastatic regional lymph nodes in breast cancer patients are a serious clinical challenge in modern oncology. According to the results of previous studies, [99mTc]Tc-(HE)3-G3 has proven to be a promising diagnostic agent in differentiating the HER2/neu receptor status in primary breast tumors (p < 0.05, Mann-Whitney test). In this regard, the purpose of this study is to explore the possibilities of using [99mTc]Tc-(HE)3-G3 to determine the HER2/neu receptor status in the metastatic axillary lymph nodes (mALNs) of breast cancer patients. The study was conducted using clinical material from 20 breast cancer patients (T2-4N1-3M0-1) before systemic therapy (10 patients with positive and 10 patients with negative HER2/neu expression in mALNs) who underwent SPECT/CT scan 4 h after the administration of [99mTc]Tc-(HE)3-G3. Morphological and immunohistochemical studies of mALNs with assessment of the HER2/neu status were performed on all patients. We found that mALN-to-background and mALN-to-latissimus dorsi muscle ratios for [99mTc]Tc-(HE)3-G3 uptake 4 h after its administration may be used for typing of the HER2/neu status in mALNs of breast cancer patients (p < 0.05, Mann-Whitney test). In that case, sensitivity and specificity for the mALN-to-background ratio were identical at 80%, with the threshold value being > 12.25.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 2","pages":"22-29"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11345088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Calcium Ions on the Electrophysiological Properties of Single ANO6 Channels. 钙离子对单个 ANO6 通道电生理特性的影响

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27338

D O Kolesnikov, E R Grigorieva, M A Nomerovskaya, D S Reshetin, A V Shalygin, E V Kaznacheyeva

{"title":"The Effect of Calcium Ions on the Electrophysiological Properties of Single ANO6 Channels.","authors":"D O Kolesnikov, E R Grigorieva, M A Nomerovskaya, D S Reshetin, A V Shalygin, E V Kaznacheyeva","doi":"10.32607/actanaturae.27338","DOIUrl":"10.32607/actanaturae.27338","url":null,"abstract":"Proteins belonging to the anoctamin (ANO) family form calcium-activated chloride channels (CaCCs). The most unusual member of this family, ANO6 (TMEM16F), simultaneously exhibits the functions of calcium-dependent scramblase and the ion channel. ANO6 affects the plasma membrane dynamics and phosphatidylserine transport; it is also involved in programmed cell death. The properties of ANO6 channels remain the subject of debate. In this study, we investigated the effect of variations in the intracellular and extracellular concentrations of calcium ions on the electrophysiological properties of endogenous ANO6 channels by recording single ANO6 channels. It has been demonstrated that (1) a high calcium concentration in an extracellular solution increases the activity of endogenous ANO6 channels, (2) the permeability of endogenous ANO6 channels for chloride ions is independent of the extracellular concentration of calcium ions, (3) that an increase in the intracellular calcium concentration leads to the activation of endogenous ANO6 channels with double amplitude, and (4) that the kinetics of the channel depend on the plasma membrane potential rather than the intracellular concentration of calcium ions. Our findings give grounds for proposing new mechanisms for the regulation of the ANO6 channel activity by calcium ions both at the inner and outer sides of the membrane.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 1","pages":"40-47"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140846448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Liposomes of Various Compositions on the Skin and Its Derivatives After II-IIIA Degree Thermal Burns. 不同成分的脂质体对 II-IIIA 度热烧伤后皮肤及其衍生物的影响

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27329

N I Pashkevich, D V Vilyanen, A F Marcinkevich, M M Borisova-Mubarakshina, S S Osochuk

引用次数: 0

ELABELA-APJ Axis Enhances Mesenchymal Stem Cell Proliferation and Migration via the METTL3/PI3K/AKT Pathway. ELABELA-APJ轴通过METTL3/PI3K/AKT途径增强间充质干细胞的增殖和迁移

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.17863

D Xu, J Fu, X Liu, Y Hong, X Chen, S Li, J Hou, K Zhang, C Zhou, C Zeng, G Zheng, H Wu, T Wang

{"title":"ELABELA-APJ Axis Enhances Mesenchymal Stem Cell Proliferation and Migration via the METTL3/PI3K/AKT Pathway.","authors":"D Xu, J Fu, X Liu, Y Hong, X Chen, S Li, J Hou, K Zhang, C Zhou, C Zeng, G Zheng, H Wu, T Wang","doi":"10.32607/actanaturae.17863","DOIUrl":"10.32607/actanaturae.17863","url":null,"abstract":"Mesenchymal stem cells (MSCs) possess a strong therapeutic potential in regenerative medicine. ELABELA (ELA) is a 32 amino acid peptide that binds to the apelin peptide jejunum receptor (APJ) to regulate cell proliferation and migration. The aim of this study was to investigate the function of ELA vis-a-vis the MSC proliferation and migration, and further explore the underlying mechanism. We demonstrated that the exogenous supplement of ELA boosts the proliferation and migration ability of MSCs, alongside improved in vitro cell viability. These capabilities were rendered moot upon APJ knockdown. In addition, ELA (5-20 μM) was shown to upregulate the expression of METTL3 in a concentrationdependent pattern, a capacity which was suppressed by APJ reduction, whereas the downregulation of METTL3 expression blocked the beneficial effects induced by ELA. ELA was also observed to upregulate the phosphorylation level of AKT. This ELA-induced activation of the PI3K/AKT pathway, however, is inhibited with knockdown of METTL3. Our data indicate that ELA could act as a promoter of MSC proliferation and migration in vitro through the APJ receptor, something which might be attributed to the activation of the METTL3/PI3K/AKT signaling pathway. Therefore, ELA is a candidate for optimizing MSC-based cell therapy, while METTL3 is a potential target for its promoting action on MSCs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 1","pages":"111-118"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140846606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptomyces phaeochromogenes BV-204, K-1115A Anthraquinone-Producing Strain: A New Protein Biosynthesis Inhibitor. Streptomyces phaeochromogenes BV-204, K-1115A Anthraquinone-Producing Strain：一种新的蛋白质合成抑制剂。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27315

A R Belik, Yu V Zakalyukina, V A Alferova, Y A Buyuklyan, I A Osterman, M V Biryukov

{"title":"Streptomyces phaeochromogenes BV-204, K-1115A Anthraquinone-Producing Strain: A New Protein Biosynthesis Inhibitor.","authors":"A R Belik, Yu V Zakalyukina, V A Alferova, Y A Buyuklyan, I A Osterman, M V Biryukov","doi":"10.32607/actanaturae.27315","DOIUrl":"10.32607/actanaturae.27315","url":null,"abstract":"In the search for new antibiotics, it is a common occurrence that already known molecules are \"rediscovered\" while new promising ones remain unnoticed. A possible solution to this problem may be the so-called \"target-oriented\" search, using special reporter microorganisms that combine increased antibiotic sensitivity with the ability to identify a molecule's damaging effect. The use of such test organisms makes it possible to discover new promising properties even in known metabolites. In this study, we used a high-throughput screening method based on the pDualrep2 dual reporter system, which combines high sensitivity through the use of modified strains of test organisms and makes it possible to easily and accurately identify the interaction mechanisms of a substance and a bacterial cell at the initial stages of screening. This reporter system is unknown in Russia and is significantly superior to its global analogues. In the system, translation inhibition induces the expression of the fluorescent protein Katushka2s, while DNA damage is induced by TurboRFP. Using pDualrep2, we have isolated and described BV-204, an S. phaeochromogenes strain producing K-1115A, the biologically active substance that we have previously described. In our study, K-1115A for the first time has demonstrated antibiotic activity and an ability to inhibit bacterial translation, which was confirmed in vitro in a cell-free translation system for FLuc mRNA. K-1115A's antibacterial activity was tested and confirmed for S. aureus (MRSA) and B. subtilis, its cytotoxicity measured against that for the HEK293 cell line. Its therapeutic index amounted to 2 and 8, respectively. The obtained results open up prospects for further study of K-1115A; so, this can be regarded as the basis for the production of semi-synthetic derivatives with improved therapeutic properties to be manufactured in dosage forms.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 1","pages":"30-39"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modern Approaches to de novo Synthesis of Extended DNA Fragments: Assembly of a Wide Repertoire of Sequences. 扩展 DNA 片段从头合成的现代方法：组装多种序列。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27362

T A Semashko, G Y Fisunov, E A Tsoy, D R Kharrasov, I K Chudinov, D V Evsyutina, G Y Shevelev, V M Govorun

引用次数: 0

A Method for the Production of Recombinant VSVs with Confirmation of Biological Activity. 一种生产重组 VSV 并确认其生物活性的方法。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27314

V D Moroz, N B Gasanov, A D Egorov, A S Malogolovkin, M O Nagornykh, E N Subcheva, E S Kolosova, A Yu Fizikova, R A Ivanov, A V Karabelsky

{"title":"A Method for the Production of Recombinant VSVs with Confirmation of Biological Activity.","authors":"V D Moroz, N B Gasanov, A D Egorov, A S Malogolovkin, M O Nagornykh, E N Subcheva, E S Kolosova, A Yu Fizikova, R A Ivanov, A V Karabelsky","doi":"10.32607/actanaturae.27314","DOIUrl":"10.32607/actanaturae.27314","url":null,"abstract":"The design of new effective cancer treatment methods is a promising and important research field in translational medicine. Oncolytic viruses can induce immunogenic cell death by activating the body's immune system to recognize tumor cells. This work presents the results for optimizing the production of recombinant vesicular stomatitis viruses (rVSVs). To ensure the assembly of viral particles, we developed the HEK293TN-T7 cell line, which stably expresses DNA-dependent RNA polymerase 7 for viral genome transcription, and obtained helper plasmids encoding viral genes under the control of the CAG promoter. The oncolytic activity of the purified virus preparation was assessed in a murine model of B16F10Red melanoma cells expressing a red fluorescent protein. The presented method makes it possible to obtain purified viral preparations with a high titer and oncolytic activity. The amplification of viral particles in a HEK293 suspension culture allows for rapid scalability. Therefore, the developed approach can be used to obtain other recombinant VSV-based oncolytic viruses for tumor immunotherapy.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 1","pages":"59-66"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-transcriptional Regulation of Gene Expression via Unproductive Splicing. 通过非生产性剪接对基因表达进行转录后调控

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27337

L G Zavileyskiy, D D Pervouchine

引用次数: 0

Ultrastructural 3D Microscopy for Biomedicine: Principles, Applications, and Perspectives. 生物医学超微结构三维显微镜：原理、应用和前景》。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27323

K E Mochalov, D S Korzhov, A V Altunina, O I Agapova, V A Oleinikov

{"title":"Ultrastructural 3D Microscopy for Biomedicine: Principles, Applications, and Perspectives.","authors":"K E Mochalov, D S Korzhov, A V Altunina, O I Agapova, V A Oleinikov","doi":"10.32607/actanaturae.27323","DOIUrl":"10.32607/actanaturae.27323","url":null,"abstract":"Modern biomedical research often requires a three-dimensional microscopic analysis of the ultrastructure of biological objects and materials. Conceptual technical and methodological solutions for three-dimensional structure reconstruction are needed to improve the conventional optical, electron, and probe microscopy methods, which to begin with allow one to obtain two-dimensional images and data. This review discusses the principles and potential applications of such techniques as serial section transmission electron microscopy; techniques based on scanning electron microscopy (SEM) (array tomography, focused ion beam SEM, and serial block-face SEM). 3D analysis techniques based on modern super-resolution optical microscopy methods are described (stochastic optical reconstruction microscopy and stimulated emission depletion microscopy), as well as ultrastructural 3D microscopy methods based on scanning probe microscopy and the feasibility of combining them with optical techniques. A comparative analysis of the advantages and shortcomings of the discussed approaches is performed.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 1","pages":"14-29"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studying Signaling Pathway Activation in TRAIL-Resistant Macrophage-Like Acute Myeloid Leukemia Cells. 研究 TRAIL 抗性巨噬细胞样急性髓性白血病细胞的信号通路激活。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-01-01 DOI: 10.32607/actanaturae.27317

Y V Lomovskaya, K S Krasnov, M I Kobyakova, A A Kolotova, A M Ermakov, A S Senotov, I S Fadeeva, E I Fetisova, A I Lomovsky, A I Zvyagina, V S Akatov, R S Fadeev

{"title":"Studying Signaling Pathway Activation in TRAIL-Resistant Macrophage-Like Acute Myeloid Leukemia Cells.","authors":"Y V Lomovskaya, K S Krasnov, M I Kobyakova, A A Kolotova, A M Ermakov, A S Senotov, I S Fadeeva, E I Fetisova, A I Lomovsky, A I Zvyagina, V S Akatov, R S Fadeev","doi":"10.32607/actanaturae.27317","DOIUrl":"10.32607/actanaturae.27317","url":null,"abstract":"Acute myeloid leukemia (AML) is a malignant neoplasm characterized by extremely low curability and survival. The inflammatory microenvironment and maturation (differentiation) of AML cells induced by it contribute to the evasion of these cells from effectors of antitumor immunity. One of the key molecular effectors of immune surveillance, the cytokine TRAIL, is considered a promising platform for developing selective anticancer drugs. Previously, under in vitro conditions of the inflammatory microenvironment (a three-dimensional high-density culture of THP-1 AML cells), we demonstrated the emergence of differentiated macrophage-like THP-1ad clones resistant to TRAIL-induced death. In the present study, constitutive activation of proinflammatory signaling pathways, associated transcription factors, and increased expression of the anti-apoptotic BIRC3 gene were observed in TRAIL-resistant macrophage-like THP-1ad AML cells. For the first time, a bioinformatic analysis of the transcriptome revealed the main regulator, the IL1B gene, which triggers proinflammatory activation and induces resistance to TRAIL in THP-1ad macrophage-like cells.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 1","pages":"48-58"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140846363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0