粮食中增殖镰刀菌产毒能力的研究。

IF 2 4区 生物学 Q4 CELL BIOLOGY
O P Gavrilova, A S Orina, T Yu Gagkaeva, N N Gogina
{"title":"粮食中增殖镰刀菌产毒能力的研究。","authors":"O P Gavrilova, A S Orina, T Yu Gagkaeva, N N Gogina","doi":"10.32607/actanaturae.27546","DOIUrl":null,"url":null,"abstract":"<p><p>The widespread fungus <i>Fusarium proliferatum</i> can infect numerous plant species and produce a range of mycotoxins, the amount of which can vary significantly. Twelve <i>F. proliferatum</i> sensu lato strains isolated from six wheat, four oat, and two maize grain samples were analyzed. The strains were identified through a phylogenetic analysis of nucleotide sequences derived from gene fragments of the translation elongation factor EF-1α, β-tubulin, and RNA polymerase II second subunit. The mating types of the strain were determined by allele-specific PCR. Secondary toxic metabolite production by the strains was quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). All twelve <i>Fusarium</i> strains formed a distinct clade alongside the <i>F. proliferatum</i> reference strains, thereby confirming the taxonomic identification. Only one idiomorph at the MAT locus in each <i>F. proliferatum</i> strain was found, indicative of heterothallic mating. The frequency of the MAT1-1 idiomorph was double that of the MAT1-2 idiomorph. The active biosynthesis of fumonisins B1 (71-6175 mg/kg), B2 (12-2661 mg/kg), and B3 (6-588 mg/kg), significant beauvericin (64-455 mg/kg), and trace amounts of moniliformin (12-6565 μg/kg) were identified across all examined <i>F. proliferatum</i> strains.</p>","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"20-28"},"PeriodicalIF":2.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011193/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Toxin-Producing Ability of Fusarium Proliferatum Strains Isolated from Grain.\",\"authors\":\"O P Gavrilova, A S Orina, T Yu Gagkaeva, N N Gogina\",\"doi\":\"10.32607/actanaturae.27546\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The widespread fungus <i>Fusarium proliferatum</i> can infect numerous plant species and produce a range of mycotoxins, the amount of which can vary significantly. Twelve <i>F. proliferatum</i> sensu lato strains isolated from six wheat, four oat, and two maize grain samples were analyzed. The strains were identified through a phylogenetic analysis of nucleotide sequences derived from gene fragments of the translation elongation factor EF-1α, β-tubulin, and RNA polymerase II second subunit. The mating types of the strain were determined by allele-specific PCR. Secondary toxic metabolite production by the strains was quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). All twelve <i>Fusarium</i> strains formed a distinct clade alongside the <i>F. proliferatum</i> reference strains, thereby confirming the taxonomic identification. Only one idiomorph at the MAT locus in each <i>F. proliferatum</i> strain was found, indicative of heterothallic mating. The frequency of the MAT1-1 idiomorph was double that of the MAT1-2 idiomorph. The active biosynthesis of fumonisins B1 (71-6175 mg/kg), B2 (12-2661 mg/kg), and B3 (6-588 mg/kg), significant beauvericin (64-455 mg/kg), and trace amounts of moniliformin (12-6565 μg/kg) were identified across all examined <i>F. proliferatum</i> strains.</p>\",\"PeriodicalId\":6989,\"journal\":{\"name\":\"Acta Naturae\",\"volume\":\"17 1\",\"pages\":\"20-28\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011193/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Naturae\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.32607/actanaturae.27546\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Naturae","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.32607/actanaturae.27546","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

广泛分布的真菌增殖镰刀菌可以感染许多植物物种并产生一系列真菌毒素,其数量差异很大。对从6种小麦、4种燕麦和2种玉米中分离得到的12株增芽枯草菌进行了分析。通过对翻译延伸因子EF-1α、β-微管蛋白和RNA聚合酶II第二亚基基因片段的核苷酸序列进行系统发育分析,鉴定了这些菌株。采用等位基因特异性PCR法测定菌株的交配类型。采用高效液相色谱-串联质谱(HPLC-MS/MS)对菌株产生的次生毒性代谢物进行定量分析。所有12株镰刀菌均与增殖镰刀菌参考菌株形成了一个不同的分支,从而证实了分类鉴定。在每个增殖力菌株的MAT位点上只发现一个特异形态,表明异源交配。MAT1-1特型的出现频率是MAT1-2特型的两倍。在所有被检测的增殖F. atum菌株中均检测到富马毒素B1 (71 ~ 6175 mg/kg)、B2 (12 ~ 2661 mg/kg)和B3 (6 ~ 588 mg/kg)的活性生物合成,以及显著的beauvericin (64 ~ 455 mg/kg)和微量的moniliformin (12 ~ 6565 μ kg)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Toxin-Producing Ability of Fusarium Proliferatum Strains Isolated from Grain.

The widespread fungus Fusarium proliferatum can infect numerous plant species and produce a range of mycotoxins, the amount of which can vary significantly. Twelve F. proliferatum sensu lato strains isolated from six wheat, four oat, and two maize grain samples were analyzed. The strains were identified through a phylogenetic analysis of nucleotide sequences derived from gene fragments of the translation elongation factor EF-1α, β-tubulin, and RNA polymerase II second subunit. The mating types of the strain were determined by allele-specific PCR. Secondary toxic metabolite production by the strains was quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). All twelve Fusarium strains formed a distinct clade alongside the F. proliferatum reference strains, thereby confirming the taxonomic identification. Only one idiomorph at the MAT locus in each F. proliferatum strain was found, indicative of heterothallic mating. The frequency of the MAT1-1 idiomorph was double that of the MAT1-2 idiomorph. The active biosynthesis of fumonisins B1 (71-6175 mg/kg), B2 (12-2661 mg/kg), and B3 (6-588 mg/kg), significant beauvericin (64-455 mg/kg), and trace amounts of moniliformin (12-6565 μg/kg) were identified across all examined F. proliferatum strains.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta Naturae
Acta Naturae 农林科学-林学
CiteScore
3.50
自引率
5.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Acta Naturae is an international journal on life sciences based in Moscow, Russia. Our goal is to present scientific work and discovery in molecular biology, biochemistry, biomedical disciplines and biotechnology. These fields represent the most important priorities for the research and engineering development both in Russia and worldwide. Acta Naturae is also a periodical for those who are curious in various aspects of biotechnological business, innovations in pharmaceutical areas, intellectual property protection and social consequences of scientific progress. The journal publishes analytical industrial surveys focused on the development of different spheres of modern life science and technology. Being a radically new and totally unique journal in Russia, Acta Naturae is useful to both representatives of fundamental research and experts in applied sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信