Acta Naturae最新文献_第4页

Evolutionary Perspectives on Human-Artificial Intelligence Convergence. 人类与人工智能融合的进化视角。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27406

B L Zybailov, G Yu Kosovsky, G V Glazko, V I Glazko, O I Skobel

{"title":"Evolutionary Perspectives on Human-Artificial Intelligence Convergence.","authors":"B L Zybailov, G Yu Kosovsky, G V Glazko, V I Glazko, O I Skobel","doi":"10.32607/actanaturae.27406","DOIUrl":"10.32607/actanaturae.27406","url":null,"abstract":"In this analytical review, we explore the potential impact of the rapid proliferation of artificial intelligence (AI) tools on the biosphere and noosphere, suggesting that the trend may lead to a transformative event that could be termed \"Human-AI integration.\" We argue that this integration could give rise to novel lifeforms, associations, and hierarchies, resulting in competitive advantages and increased complexity of structural organizations within both the biosphere and noosphere. Our central premise emphasizes the importance of human-AI integration as a global adaptive response crucial for our civilization's survival amidst a rapidly changing environment. The convergence may initially manifest itself through symbiotic, endosymbiotic, or other mutualistic relationships, such as domestication, contingent on the rate at which AI systems achieve autonomy and develop survival instincts akin to those of biological organisms. We investigate potential drivers of these scenarios, addressing the ethical and existential challenges arising from the AI-driven transformation of the biosphere and noosphere, and considering potential trade-offs. Additionally, we discuss the application of complexity and the balance between competition and cooperation to better comprehend and navigate these transformative scenarios.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"4-17"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Structure of the Components of the PolyADP-Ribosylation System in Fusarium Fungi and Evaluating the Expression Dynamics of Its Key Genes. 研究镰刀菌中 PolyADP-Ribosylation 系统的组成结构并评估其关键基因的表达动态。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27450

A A Stakheev, R R Kutukov, M E Taliansky, S K Zavriev

{"title":"Investigating the Structure of the Components of the PolyADP-Ribosylation System in Fusarium Fungi and Evaluating the Expression Dynamics of Its Key Genes.","authors":"A A Stakheev, R R Kutukov, M E Taliansky, S K Zavriev","doi":"10.32607/actanaturae.27450","DOIUrl":"10.32607/actanaturae.27450","url":null,"abstract":"Poly(ADP-ribose) polymerase (PARP) is the key enzyme in polyADP-ribosylation, one of the main post-translational modifications. This enzyme is abundant in eukaryotic organisms. However, information on the PARP structure and its functions in members of the Fungi kingdom is very limited. In this study, we performed a bioinformatic search for homologs of PARP and its antagonist, PARG, in the genomes of four Fusarium strains using their whole-genome sequences annotated and deposited in databases. The F. graminearum PH-1, F. proliferatum ET-1, and F. oxysporum Fo47 strains were shown to possess a single homolog of both PARP and PARG. In addition, the F. oxysporum f. sp. lycopersici strain 4287 contained four additional proteins comprising PARP catalytic domains whose structure was different from that of the remaining identified homologs. Partial nucleotide sequences encoding the catalytic domains of the PARP and PARG homologs were determined in 11 strains of 9 Fusarium species deposited in all-Russian collections, and the phylogenetic properties of the analyzed genes were evaluated. In the toxigenic F. graminearum strain, we demonstrated up-regulation of the gene encoding the PARP homolog upon culturing under conditions stimulating the production of the DON mycotoxin, as well as up-regulation of the gene encoding PARG at later stages of growth. These findings indirectly indicate involvement of the polyADP-ribosylation system in the regulation of the genes responsible for DON biosynthesis.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"83-92"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142650545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abundance of Tumor-Infiltrating B Cells in Human Epithelial Malignancies. 人类上皮恶性肿瘤中肿瘤浸润 B 细胞的丰富性

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27353

E A Petrov, D M Malabuiok, H Zheng, Yu A Mokrushina, V A Abrikosova, Yu B Kuzmin, P V Tzarapaev, S O Kochkina, I V Eltsov, V D Knorre, I V Smirnov, S S Terekhov, Z Mamedli, N E Kushlinskii, D V Rogozhin, V B Matveev, P V Kononets, I S Stilidi, H Zhang, A G Gabibov

{"title":"Abundance of Tumor-Infiltrating B Cells in Human Epithelial Malignancies.","authors":"E A Petrov, D M Malabuiok, H Zheng, Yu A Mokrushina, V A Abrikosova, Yu B Kuzmin, P V Tzarapaev, S O Kochkina, I V Eltsov, V D Knorre, I V Smirnov, S S Terekhov, Z Mamedli, N E Kushlinskii, D V Rogozhin, V B Matveev, P V Kononets, I S Stilidi, H Zhang, A G Gabibov","doi":"10.32607/actanaturae.27353","DOIUrl":"10.32607/actanaturae.27353","url":null,"abstract":"Cancer is a major global health problem. The type of malignant neoplasm and the potency of the immune response against tumors are two of the key factors influencing the outcome of the disease. The degree of tumor infiltration by lymphocytes plays an important role in antitumor response development, generally correlating with a favorable prognosis of treatment for certain cancers. We analyzed the abundance of tumor-infiltrating B cells (TIBs) in solid tumors of different cancers. TIBs were shown to be more abundant in colon and sigmoid colon cancer samples compared with cecal, rectal, and kidney cancer samples. The median and interquartile range of the TIB fraction were 11.5% and 4-20% in colon cancer, 6% and 3-11% in sigmoid colon cancer, 2.7% and 0.7-3.7% in cecal cancer, 2.5% and 0.9-3.6% in rectal cancer, 1.4% and 1.0-2.3% in kidney cancer, and 3.0% and 1.8-12% in lung cancer, respectively. However, there were no significant differences in the abundance of TIBs among samples at different stages of the cancer. Hence, investigation of the B cell response in colon cancer is of particular interest, since increased quantities of TIBs may indicate the existence of immunogenic tumor markers or the cell-cell interactions involved in disease progression. We believe that studying the diversity of TIBs in colon cancer will increaseour understanding of the mechanisms of the disease, contributing to the identification of new molecular targets for targeted oncotherapy.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"67-73"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Activation of the Expression of Growth Factor Genes in Expi293F Human Cells Using CRISPR/Cas9-SAM Technology Increases Their Proliferation. 利用 CRISPR/Cas9-SAM 技术特异性激活 Expi293F 人体细胞中生长因子基因的表达可增加其增殖。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27415

P A Bobrovsky, E N Grafskaia, D D Kharlampieva, V A Manuvera, V N Lazarev

{"title":"Specific Activation of the Expression of Growth Factor Genes in Expi293F Human Cells Using CRISPR/Cas9-SAM Technology Increases Their Proliferation.","authors":"P A Bobrovsky, E N Grafskaia, D D Kharlampieva, V A Manuvera, V N Lazarev","doi":"10.32607/actanaturae.27415","DOIUrl":"10.32607/actanaturae.27415","url":null,"abstract":"Human cell lines play an important role in biotechnology and pharmacology. For them to grow, they need complex nutrient media containing signaling proteins - growth factors. We have tested a new approach that reduces the need of cultured human cell lines for exogenous growth factors. This approach is based on the generation of a modified cell with a selectively activated gene expression of one of the endogenous growth factors: IGF-1, FGF-2, or EIF3I. We modified the Expi293F cell line, a HEK293 cell line variant widely used in the production of recombinant proteins. Gene expression of the selected growth factors in these cells was activated using CRISPR/Cas9 technology with the synergistic activation mediators CRISPR/Cas9-SAM, which increased the expression of the selected genes at both mRNA and protein levels. Upon culturing under standard conditions, the modified lines exhibited increased proliferation. A synergistic effect was observed in co-culture of the three modified lines. In our opinion, these results indicate that this approach is promising for efficient modification of cell lines used in biotechnology.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"25-37"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Lesions with a Fluorescein Carbamoyl Group As Analogs of Bulky Lesions Removable by Nucleotide Excision Repair: A Comparative Study on Properties. 用荧光素氨基甲酰基合成可通过核苷酸切除修复去除的大块病变的类似物：性质比较研究。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27419

A A Popov, V M Golyshev, L S Koroleva, K D Nazarov, R O Anarbaev, I O Petruseva

{"title":"Synthetic Lesions with a Fluorescein Carbamoyl Group As Analogs of Bulky Lesions Removable by Nucleotide Excision Repair: A Comparative Study on Properties.","authors":"A A Popov, V M Golyshev, L S Koroleva, K D Nazarov, R O Anarbaev, I O Petruseva","doi":"10.32607/actanaturae.27419","DOIUrl":"10.32607/actanaturae.27419","url":null,"abstract":"Mammalian nucleotide excision repair (NER), known for its broad substrate specificity, is responsible for removal of bulky lesions from DNA. Over 30 proteins are involved in NER, which includes two distinct pathways: global genome NER and transcription-coupled repair. The complexity of these processes, the use of extended DNA substrates, and the presence of bulky DNA lesions induced by chemotherapy have driven researchers to seek more effective methods by which to assess NER activity, as well as to develop model DNAs that serve as efficient substrates for studying lesion removal. In this work, we conducted a comparative analysis of model DNAs containing bulky lesions. One of these lesions, N-[6-{5(6)-fluoresceinylcarbamoyl}hexanoyl]-3-amino-1,2-propanediol (nFluL), is known to be efficiently recognized and excised by NER. The second lesion, N-[6-{5(6)-fluoresceinylcarbamoyl}]-3-amino-1,2-propanediol (nFluS), has not previously been tested as a substrate for NER. To evaluate the efficiency of lesion excision, a 3'-terminal labeling method was employed to analyze the excision products. The results showed that nFluS is removed approximately twice as efficiently as nFluL. Comparative analyses of the effects of nFluL and nFluS on the geometry and thermal stability of DNA duplexes - combined with spectrophotometric and spectrofluorimetric titrations of these DNAs with complementary strands - were performed next. They revealed that the absence of an extended flexible linker in nFluS alters the interaction of the bulky fluorescein moiety with neighboring nitrogenous bases in double-stranded DNA. This absence is associated with the enhanced efficiency of excision of nFluS, making it a more effective synthetic analog for studying bulky-lesion removal in model DNA substrates.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 3","pages":"74-82"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of the Reaction of Andalusicin Lantibiotic Modification by Lanthionine Synthetase AncKC in a Heterologous Escherichia coli System. 在异源大肠杆菌系统中重建鹅掌楸碱合成酶 AncKC 的安达鲁西辛抗生素修饰反应

IF 2 4区生物学

Acta Naturae Pub Date : 2024-07-01 DOI: 10.32607/actanaturae.27347

N Z Mirzoeva, S O Pipiya, Yu A Mokrushina, M V Serebryakova, A A Grigoreva, S A Dubiley, S S Terekhov, I V Smirnov

引用次数: 0

A Vector Nanoplatform for the Bioimaging of Deep-Seated Tumors. 用于深部肿瘤生物成像的载体纳米平台

IF 2 4区生物学

Acta Naturae Pub Date : 2024-04-01 DOI: 10.32607/actanaturae.27425

E I Shramova, S M Deyev, G M Proshkina

{"title":"A Vector Nanoplatform for the Bioimaging of Deep-Seated Tumors.","authors":"E I Shramova, S M Deyev, G M Proshkina","doi":"10.32607/actanaturae.27425","DOIUrl":"10.32607/actanaturae.27425","url":null,"abstract":"Today, in preclinical studies, optical bioimaging based on luminescence and fluorescence is indispensable in studying the development of neoplastic transformations, the proliferative activity of the tumor, its metastatic potential, as well as the therapeutic effect of antitumor agents. In order to expand the capabilities of optical imaging, sensors based on the bioluminescence resonance energy transfer (BRET) mechanism and, therefore, independent of an external light source are being developed. A targeted nanoplatform based on HER2-specific liposomes whose internal environment contains a genetically encoded BRET sensor was developed in this study to visualize deep-seated tumors characterized by overexpression of human epidermal growth factor receptor type 2 (HER2). The BRET sensor is a hybrid protein consisting of the highly catalytic luciferase NanoLuc (an energy donor) and a LSSmKate1 red fluorescent protein with a large Stokes shift (an energy acceptor). During the bioimaging of disseminated intraperitoneal tumors formed by HER2-positive SKOV3.ip1cells of serous ovarian cystadenocarcinoma, it was shown that the developed system is applicable in detecting deep-seated tumors of a certain molecular profile. The developed system can become an efficient platform for optimizing preclinical studies of novel targeted drugs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 2","pages":"72-81"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11345090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Use of Common Marmosets (Callithrix jacchus) in Preclinical Trials of Antiviral Vaccines. 在抗病毒疫苗临床前试验中试验性使用普通狨猴（Callithrix jacchus）。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-04-01 DOI: 10.32607/actanaturae.27372

I V Gordeychuk, O S Gancharova, S A Gulyaev, T V Gulyaeva, A S Zhitkevich, D V Avdoshina, A V Moroz, A S Lunin, S E Sotskova, E A Korduban, A I Tukhvatulin, E O Bayurova, A A Ishmukhametov

引用次数: 0

Molecular Mechanisms of Drosophila Hematopoiesis. 果蝇造血的分子机制。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-04-01 DOI: 10.32607/actanaturae.27410

S A Sinenko

引用次数: 0

Dihydroquercetin-Loaded Liposomes Change Fibrous Tissue Distribution in the Bleomycin-Induced Fibrosis Model. 二氢槲皮素脂质体改变博莱霉素诱导纤维化模型中的纤维组织分布

IF 2 4区生物学

Acta Naturae Pub Date : 2024-04-01 DOI: 10.32607/actanaturae.27440

E V Ivanov, M R Akhmetshina, A R Gizatulina, M V Gulyaev, O S Pavlova, Y A Pirogov, S A Gavrilova

{"title":"Dihydroquercetin-Loaded Liposomes Change Fibrous Tissue Distribution in the Bleomycin-Induced Fibrosis Model.","authors":"E V Ivanov, M R Akhmetshina, A R Gizatulina, M V Gulyaev, O S Pavlova, Y A Pirogov, S A Gavrilova","doi":"10.32607/actanaturae.27440","DOIUrl":"10.32607/actanaturae.27440","url":null,"abstract":"The effects of the antioxidant dihydroquercetin (DHQ) were studied in a model of pulmonary fibrosis. DHQ penetration into the lesion was facilitated by encapsulation into liposomes. Pulmonary fibrosis was modeled in rats by intratracheal injection of bleomycin. For the first 7 days, the rats in the treatment group received a liposomal emulsion with DHQ, while in the comparator group rats received saline. In the control group, intact rats did not receive any exposure. Thirty days after the initiation, lung function and the pathological lesion volume were assessed by 7T 1H MRI and the lungs were taken for histologic examination. The proportion of fibrous tissue was counted by Masson's trichrome staining. Both experimental groups were characterized by a significant functional pulmonary deficiency, with low mortality and a small lesion area. In the rats treated with DHQ, the distribution of fibrous tissue was significantly altered. Significantly more fibrous tissue was found in the center of the lesion, while significantly less was in the interstitial space of alveoli. Lung density at the same time was lower in the treated lungs. Dihydroquercetin encapsulated in liposomes affects the mechanisms of bleomycin-induced pulmonary fibrosis progression in rats. While accelerated fibrosis of the lesion can restrict inflammatory processes, delayed fibrosis of the interstitium can further improve the functional state of the lungs.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 2","pages":"40-49"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11345094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0