Acta Naturae最新文献_第4页

The Parameters of Long-Term Humoral Immunity Induced by a Single Injection of the Sputnik Light Vaccine Among Noninfected Volunteers and Those Infected with SARS-CoV-2. 非感染志愿者和SARS-CoV-2感染者单次注射Sputnik轻型疫苗长期体液免疫参数的研究

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27529

F M Izhaeva, A I Tukhvatulin, A S Dzharullaeva, I V Dolzhikova, O V Zubkova, D V Shcheblyakov, N L Lubenets, E A Smolyarchuk, T G Zubkova, A S Erokhova, I M Cherenkova, K M Bukhtin, O Popova, N M Tukhvatulina, B S Naroditsky, D Y Logunov, A L Gintsburg

{"title":"The Parameters of Long-Term Humoral Immunity Induced by a Single Injection of the Sputnik Light Vaccine Among Noninfected Volunteers and Those Infected with SARS-CoV-2.","authors":"F M Izhaeva, A I Tukhvatulin, A S Dzharullaeva, I V Dolzhikova, O V Zubkova, D V Shcheblyakov, N L Lubenets, E A Smolyarchuk, T G Zubkova, A S Erokhova, I M Cherenkova, K M Bukhtin, O Popova, N M Tukhvatulina, B S Naroditsky, D Y Logunov, A L Gintsburg","doi":"10.32607/actanaturae.27529","DOIUrl":"https://doi.org/10.32607/actanaturae.27529","url":null,"abstract":"Although the immunogenicity of clinically approved COVID-19 vaccines remains under intensive investigation, little is still known about the parameters of long-term immune responses. In this paper, we present for the first time the parameters of humoral immunity studied in the phase 1-2 open-label clinical trial of the Sputnik Light vaccine, with a special focus on late follow-up time points (90 and 180 days). For the most accurate assessment of the parameters of humoral post-vaccination immunity (titer and avidity index of antigen-specific antibodies against the RBD domain of SARS-CoV-2), we conducted an additional analysis that allowed us to triage volunteers with immunity formed only in response to vaccination, as well as those with hybrid immunity (infected with SARS-CoV-2 before and after vaccination). The findings indicate that single-shot vaccination with the Sputnik Light vaccine induces a durable (seroconversion 73% on day 180) and mature humoral immunity. Natural immunization as a result of the SARS-CoV-2 infection leads to significant changes in the studied parameters of post-vaccination immunity.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"52-63"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoregulatory Enzymes. 免疫调节酶。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27549

S V Kupriyanov, K M Kirilenko, D N Starkov

引用次数: 0

Inhibition of HBV Replication by a Fully Humanized Neutralizing Antibody In Vivo and In Vitro. 一种完全人源化中和抗体在体内和体外抑制HBV复制。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27457

Zhipeng Zhang, Yanqin Ma, Yan He, Dong Wang, Huaien Song, Kun Yue, Xiaomei Zhang

{"title":"Inhibition of HBV Replication by a Fully Humanized Neutralizing Antibody In Vivo and In Vitro.","authors":"Zhipeng Zhang, Yanqin Ma, Yan He, Dong Wang, Huaien Song, Kun Yue, Xiaomei Zhang","doi":"10.32607/actanaturae.27457","DOIUrl":"https://doi.org/10.32607/actanaturae.27457","url":null,"abstract":"Neutralizing antibodies are capable of specifically binding to the HBsAg virus, thereby preventing HBV infection and subsequently reducing viral antigen load in both the liver and systemic circulation. This has significant implications for restoring the postnatal immune function. By utilizing the phage antibody library technology, we successfully screened a fully humanized neutralizing antibody targeting the hepatitis B surface antigen. The antiviral activity was assessed in primary human hepatocytes (PHHs) by determining the EC50 values for HBeAg and HBsAg biomarkers in HBV types B, C, and D; no cytotoxicity was observed within the tested concentration range. Furthermore, HT-102 exhibited no ADCC effect but displayed a weak CDC effect along with a dose-dependent response. We established an AAV/HBV mouse model and observed significant dose-dependent reduction in HBsAg and HBV DNA levels for both the medium-dose and highdose groups. The immunohistochemical staining data showed dose-dependent reduction in HBsAg expression in the liver, with high-dose group exhibiting minimal positive expression. Finally, a mild immune response was induced, while reducing the burden of antigen-antibody complexes circulating within the system. Consequently, strain on the patient's immune system was alleviated by effectively slowing down CD8+T lymphocyte depletion, and functional cure was ultimately achieved as intended.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"97-105"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodobacter capsulatus PG Lipopolysaccharide As a Potential Blocker of Toll-like Receptor 2 and 4 Activation. 荚膜红杆菌PG脂多糖作为toll样受体2和4激活的潜在阻断剂。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27555

S V Zubova, Ya V Radzyukevich, N I Kosyakova, I R Prokhorenko

引用次数: 0

Intergeneric Introgression Enhances the Adaptive Potential of Nine-Spined Stickleback (Pungitius pungitius). 属间渗透增强九棘棘鱼（Pungitius Pungitius）的适应性。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27528

A V Nedoluzhko, F S Sharko, S M Rastorguev

引用次数: 0

Activation of the ERK1/2 Molecular Pathways and Its Relation to the Pathogenicity of Human Malignant Tumors. ERK1/2分子通路的激活及其与人类恶性肿瘤致病性的关系

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27497

A G Emelyanova, M A Zolotovskaia, E V Poddubskaya, A A Modestov, V S Prassolov, D V Kuzmin, A A Buzdin

{"title":"Activation of the ERK1/2 Molecular Pathways and Its Relation to the Pathogenicity of Human Malignant Tumors.","authors":"A G Emelyanova, M A Zolotovskaia, E V Poddubskaya, A A Modestov, V S Prassolov, D V Kuzmin, A A Buzdin","doi":"10.32607/actanaturae.27497","DOIUrl":"https://doi.org/10.32607/actanaturae.27497","url":null,"abstract":"Mitogen-activated protein kinases, ERK1/2 (MAPK3/1), play a key role in the regulation of cell growth, differentiation, and apoptosis. We have previously presented evidence proving that activation of the ERK1/2 axis in cancer cells following the administration of therapeutics leads to the overexpression of growth factor receptors and drug resistance. Recently, we have proposed a new bioinformatic technique that enables direct construction of interactome network-based molecular pathways for gene products of interest, as well as quantitation of their activation levels using high-throughput gene expression data. In this study, we, for the first time, algorithmically constructed ERK1/2 molecular pathways and investigated how their activation levels (PALs) affect survival and responsiveness to targeted drugs at the pan-cancer level based on transcriptomic data. We examined a total of 11 287 human tumor profiles from 31 types of cancer, drawn from 53 of our previously published and other literature datasets, looking at patient survival and clinical response to 29 chemo- and targeted therapy regimens. We found that activation of the ERK1/2 pathways has different prognostic significance depending on cancer type. In glioblastoma, sarcoma, lung, kidney, bladder, gastric, colon, and several other cancer types, ERK pathway activation was associated with worse survival. In contrast, the same phenomenon was associated with a better chance of survival in HER2+, luminal A and luminal B breast cancer, and uterine corpus cancer. These trends were consistent with treatment response analysis. At the same time, we found significantly worse associations with the expression levels of individual MAPK1 and MAPK3 genes: hence, ERK1/2 pathway activation levels can be considered putative biomarkers for predicting clinical outcomes and selecting new personalized treatment strategies, such as the use of MAPK inhibitors.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"36-51"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous Production of Antimicrobial Peptides in Yeast Allows for Massive Assessment of the Activity of DNA-Encoded Antimicrobials In Situ. 在酵母中异种生产抗菌肽允许大量评估dna编码抗菌素的活性。

IF 2 4区生物学

Acta Naturae Pub Date : 2025-01-01 DOI: 10.32607/actanaturae.27355

S O Pipiya, A O Ivanova, Yu A Mokrushina, I E Eliseev, A G Gabibov, I V Smirnov, S S Terekhov

{"title":"Heterologous Production of Antimicrobial Peptides in Yeast Allows for Massive Assessment of the Activity of DNA-Encoded Antimicrobials In Situ.","authors":"S O Pipiya, A O Ivanova, Yu A Mokrushina, I E Eliseev, A G Gabibov, I V Smirnov, S S Terekhov","doi":"10.32607/actanaturae.27355","DOIUrl":"https://doi.org/10.32607/actanaturae.27355","url":null,"abstract":"Antibiotic resistance threatens global healthcare. In clinical practice, conventional antibiotics are becoming gradually less effective. Moreover, the introduction of new antimicrobial agents into clinical practice leads to the emergence of resistant pathogenic strains within just a few years. Hence, the development of platforms for massive creation and screening of new antimicrobial agents is of particular importance. Massive parallel screening will greatly reduce the time required to identify the most promising drug candidates. Meanwhile, DNA-encoded antimicrobial agents offer unique opportunities for the high-throughput development of new antibiotics. Here, the yeast Pichia pastoris was engineered to produce a panel of antimicrobial peptides (AMPs), followed by high-throughput screening of AMP producers that inhibit bacterial growth in situ. Yeast clones producing thanatin and protegrin-1 exhibited the highest level of antimicrobial activity among the panel of AMPs under investigation. The production level of recombinant thanatin was significantly higher than that of protegrin-1, which correlates with its low toxicity. The designed technique of massive assessment of the activity of DNA-encoded antimicrobial agents enables the identification of drug candidates with an increased therapeutic index. Further development of methods for a rational design of artificial diversity in AMPs, followed by deep functional profiling of antimicrobial activity, will yield new AMPs with improved therapeutic characteristics.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"17 1","pages":"71-77"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platforms for the Search for New Antimicrobial Agents Using In Vivo C. elegans Models. 利用线虫体内模型寻找新的抗菌药物的平台。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27348

A I Kalganova, I E Eliseev, I V Smirnov, S S Terekhov

引用次数: 0

Recombinant VSVs: A Promising Tool for Virotherapy. 重组vsv：一种有前途的病毒治疗工具。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27501

K A Vorona, V D Moroz, N B Gasanov, A V Karabelsky

引用次数: 0

Embryonic Stem Cell Differentiation to Definitive Endoderm As a Model of Heterogeneity Onset During Germ Layer Specification. 胚胎干细胞向最终内胚层分化的异质性模型。

IF 2 4区生物学

Acta Naturae Pub Date : 2024-10-01 DOI: 10.32607/actanaturae.27510

M N Gordeev, A S Zinovyeva, E E Petrenko, E V Lomert, N D Aksenov, A N Tomilin, E I Bakhmet

{"title":"Embryonic Stem Cell Differentiation to Definitive Endoderm As a Model of Heterogeneity Onset During Germ Layer Specification.","authors":"M N Gordeev, A S Zinovyeva, E E Petrenko, E V Lomert, N D Aksenov, A N Tomilin, E I Bakhmet","doi":"10.32607/actanaturae.27510","DOIUrl":"10.32607/actanaturae.27510","url":null,"abstract":"Embryonic stem cells (ESCs) hold great promise for regenerative medicine thanks to their ability to self-renew and differentiate into somatic cells and the germline. ESCs correspond to pluripotent epiblast - the tissue from which the following three germ layers originate during embryonic gastrulation: the ectoderm, mesoderm, and endoderm. Importantly, ESCs can be induced to differentiate toward various cell types by varying culture conditions, which can be exploited for in vitro modeling of developmental processes such as gastrulation. The classical model of gastrulation postulates that mesoderm and endoderm specification is made possible through the FGF-, BMP-, Wnt-, and Nodal-signaling gradients. Hence, it can be expected that one of these signals should direct ESC differentiation towards specific germ layers. However, ESC specification appears to be more complicated, and the same signal can be interpreted differently depending on the readout. In this research, using chemically defined culture conditions, homogeneous naïve ESCs as a starting cell population, and the Foxa2 gene-driven EGFP reporter tool, we established a robust model of definitive endoderm (DE) specification. This in vitro model features formative pluripotency as an intermediate state acquired by the epiblast in vivo shortly after implantation. Despite the initially homogeneous state of the cells in the model and high Activin concentration during endodermal specification, there remains a cell subpopulation that does not reach the endodermal state. This simple model developed by us can be used to study the origins of cellular heterogeneity during germ layer specification.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"16 4","pages":"62-72"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0