Alterations in the Expression of Proprotein Convertase Genes in Human Esophagus Squamous Cell Carcinomas.

IF 2 4区 生物学 Q4 CELL BIOLOGY
A A Komissarov, M V Zinovyeva, A V Sass, T V Vinogradova, S I Koshechkin, V V Demkin, I B Zborovskaya, S V Kostrov, I V Demidyuk
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引用次数: 0

Abstract

Proprotein convertases (PCs) constitute an enzyme family that includes nine highly specific human subtilisin-like serine proteases. It is known that the PCs mRNA levels vary in tumors, and that these proteases are involved in carcinogenesis. Thus, PCs may be considered as potential markers for typing and predicting the course of the disease, as well as potential targets for therapy. We used quantitative real-time PCR to evaluate the expression levels of PC genes in the paired samples of tumor and adjacent normal tissues derived from 19 patients with esophageal squamous cell carcinomas. We observed a significant enrichment of PCSK6, PCSK9, MBTPS1, and FURIN mRNAs in the tumor tissue, which may be indication of the involvement of these PCs in the development and progression of esophageal cancers. Additionally, cluster analysis of PC expression alteration patterns in tumor compared to normal adjacent tissues (esophageal and previously analyzed lung tissue samples) revealed a limited set of scenarios for the changes in PC expression. These scenarios are implemented during malignant transformation of lung and esophagus cells, as well as, probably, the cells of other organs. These findings indicate that PC genes may be important markers of human cancers.

人食管鳞状细胞癌中蛋白转化酶基因表达的改变。
蛋白转化酶(PCs)构成了一个酶家族,包括9种高度特异性的人类枯草杆菌样丝氨酸蛋白酶。已知PCs mRNA水平在肿瘤中变化,并且这些蛋白酶参与了癌变。因此,PCs可能被认为是分型和预测疾病进程的潜在标记物,以及潜在的治疗靶点。我们采用实时荧光定量PCR技术对19例食管鳞状细胞癌患者肿瘤及邻近正常组织配对样本中PC基因的表达水平进行了检测。我们观察到肿瘤组织中PCSK6、PCSK9、MBTPS1和FURIN mrna的显著富集,这可能表明这些pc参与了食管癌的发生和进展。此外,与正常邻近组织(食管和先前分析的肺组织样本)相比,肿瘤中PC表达改变模式的聚类分析揭示了PC表达变化的有限场景。这些情况发生在肺和食道细胞的恶性转化过程中,也可能发生在其他器官的细胞中。这些发现表明PC基因可能是人类癌症的重要标志。
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来源期刊
Acta Naturae
Acta Naturae 农林科学-林学
CiteScore
3.50
自引率
5.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Acta Naturae is an international journal on life sciences based in Moscow, Russia. Our goal is to present scientific work and discovery in molecular biology, biochemistry, biomedical disciplines and biotechnology. These fields represent the most important priorities for the research and engineering development both in Russia and worldwide. Acta Naturae is also a periodical for those who are curious in various aspects of biotechnological business, innovations in pharmaceutical areas, intellectual property protection and social consequences of scientific progress. The journal publishes analytical industrial surveys focused on the development of different spheres of modern life science and technology. Being a radically new and totally unique journal in Russia, Acta Naturae is useful to both representatives of fundamental research and experts in applied sciences.
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